AlfalfaGEDB | MS.gene050903

M. sativa cultivar XinJiangDaYe / MS.gene050903

KEGG: -
GO: -
PFAM: PF05498.14

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050903.t1	XP_003626248.1	100	108	0	0	1	108	19	126	4.60E-56	226.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050903.t1	Q8L9P8	84.2	76	12	0	33	108	40	115	5.2e-32	138.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050903.t1	A2Q327	100.0	108	0	0	1	108	19	126	3.3e-56	226.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050903	MS.gene07104	0.813946	1.98E-51	-1.69E-46
MS.gene050903	MS.gene07105	0.81681	4.56E-52	-1.69E-46
MS.gene050903	MS.gene07107	0.815681	8.16E-52	-1.69E-46
MS.gene050903	MS.gene072495	0.956777	1.32E-114	-1.69E-46
MS.gene050903	MS.gene07630	0.817023	4.09E-52	-1.69E-46
MS.gene050903	MS.gene23114	0.996088	2.89E-223	-1.69E-46
MS.gene050903	MS.gene30549	0.802617	5.15E-49	-1.69E-46
MS.gene050903	MS.gene68707	0.804148	2.48E-49	-1.69E-46
MS.gene050903	MS.gene71797	0.810772	9.76E-51	-1.69E-46
MS.gene050903	MS.gene78575	0.927284	1.46E-91	-1.69E-46
MS.gene050903	MS.gene90557	0.819478	1.14E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050903.t1	MTR_7g113080	100.000	108	0	0	1	108	19	126	3.21e-79	228
MS.gene050903.t1	MTR_4g119860	60.360	111	37	2	1	108	19	125	3.32e-40	130
MS.gene050903.t1	MTR_7g108600	56.481	108	40	3	4	109	15	117	4.55e-35	116
MS.gene050903.t1	MTR_3g084350	53.247	77	32	3	32	107	53	126	4.46e-20	79.3
MS.gene050903.t1	MTR_4g088685	56.250	64	26	1	46	107	44	107	2.24e-19	76.6
MS.gene050903.t1	MTR_6g060260	48.485	66	29	1	42	107	41	101	1.11e-15	67.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050903.t1	AT4G15800	84.211	76	12	0	33	108	40	115	1.13e-43	138
MS.gene050903.t1	AT1G02900	60.909	110	39	2	1	109	14	120	2.14e-41	132
MS.gene050903.t1	AT3G16570	65.556	90	17	2	33	108	47	136	2.14e-33	113
MS.gene050903.t1	AT3G16570	65.556	90	17	2	33	108	47	136	2.14e-33	113
MS.gene050903.t1	AT3G16570	65.556	90	17	2	33	108	47	136	2.14e-33	113
MS.gene050903.t1	AT3G05490	81.034	58	11	0	51	108	61	118	2.77e-30	104
MS.gene050903.t1	AT2G33775	57.971	69	28	1	40	107	37	105	4.18e-23	86.3
MS.gene050903.t1	AT1G28270	55.072	69	30	1	40	107	37	105	1.73e-19	77.0
MS.gene050903.t1	AT4G13950	57.812	64	25	2	45	107	42	104	4.49e-18	73.6
MS.gene050903.t1	AT3G23805	60.714	56	20	2	53	107	55	109	1.14e-15	67.4
MS.gene050903.t1	AT5G67070	51.282	78	27	5	42	108	51	128	3.10e-12	58.9

Find 29 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCACTGCATCCACGGCTGTA+AGG	0.445471	7.1:-3290398	None:intergenic
AGTTTGATAATGAAATCAAC+AGG	0.461525	7.1:+3290261	MS.gene050903:CDS
CGCACCATAGCTGATGTACT+TGG	0.465658	7.1:-3290300	None:intergenic
CACTGCATCCACGGCTGTAA+GGG	0.481328	7.1:-3290397	None:intergenic
CAATTGCAGACCCGGTGCTC+AGG	0.488139	7.1:+3290370	MS.gene050903:CDS
CCTCCAGCTGCATCTACTGT+TGG	0.503199	7.1:-3290137	None:intergenic
GCAGCTGGAGGATTCGAACT+TGG	0.512453	7.1:+3290149	MS.gene050903:CDS
GAAGCACCACGGCGAGAACA+AGG	0.526826	7.1:-3290341	None:intergenic
GTAAGGGTTAGCCTGAGCAC+CGG	0.526971	7.1:-3290381	None:intergenic
ATCAGCGATGGAACCTTCGC+AGG	0.533132	7.1:-3290207	None:intergenic
TCTCCAACAGTAGATGCAGC+TGG	0.538924	7.1:+3290134	MS.gene050903:CDS
TCTTACTACAATTGCAGACC+CGG	0.540294	7.1:+3290362	MS.gene050903:CDS
AGGATTCGAACTTGGAGGAA+TGG	0.547500	7.1:+3290157	MS.gene050903:CDS
GCTGGAGGATTCGAACTTGG+AGG	0.550332	7.1:+3290152	MS.gene050903:CDS
TCGAACTTGGAGGAATGGAA+TGG	0.552877	7.1:+3290162	MS.gene050903:CDS
TCAGCTATGGTGCGCTTCAG+AGG	0.563424	7.1:+3290309	MS.gene050903:CDS
GCAGTGCTATTACGAGGTGC+AGG	0.566649	7.1:+3290414	MS.gene050903:CDS
TCGTAATAGCACTGCATCCA+CGG	0.577260	7.1:-3290406	None:intergenic
TAAGGGTTAGCCTGAGCACC+GGG	0.580885	7.1:-3290380	None:intergenic
TTGTAACATGCAATCAGCGA+TGG	0.586357	7.1:-3290219	None:intergenic
CAGGCTAACCCTTACAGCCG+TGG	0.595858	7.1:+3290389	MS.gene050903:CDS
ACGACCAAGTACATCAGCTA+TGG	0.604031	7.1:+3290296	MS.gene050903:CDS
AGCGATGGAACCTTCGCAGG+TGG	0.625016	7.1:-3290204	None:intergenic
AAGCACCACGGCGAGAACAA+GGG	0.626487	7.1:-3290340	None:intergenic
ACTGTCCCTTGTTCTCGCCG+TGG	0.647098	7.1:+3290335	MS.gene050903:CDS
CCAACAGTAGATGCAGCTGG+AGG	0.701563	7.1:+3290137	MS.gene050903:CDS
GCTGATTGCATGTTACAACA+AGG	0.712911	7.1:+3290224	MS.gene050903:CDS
GTGGATGCAGTGCTATTACG+AGG	0.733772	7.1:+3290408	MS.gene050903:CDS
AATTGTAGTAAGAAGCACCA+CGG	0.751980	7.1:-3290352	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AGTTTGATAATGAAATCAAC+AGG	+	chr7.1:3290261-3290280	MS.gene050903:CDS	25.0%
!!	AATTGTAGTAAGAAGCACCA+CGG	-	chr7.1:3290355-3290374	None:intergenic	35.0%
	GCTGATTGCATGTTACAACA+AGG	+	chr7.1:3290224-3290243	MS.gene050903:CDS	40.0%
	TCTTACTACAATTGCAGACC+CGG	+	chr7.1:3290362-3290381	MS.gene050903:CDS	40.0%
	TTGTAACATGCAATCAGCGA+TGG	-	chr7.1:3290222-3290241	None:intergenic	40.0%
	ACGACCAAGTACATCAGCTA+TGG	+	chr7.1:3290296-3290315	MS.gene050903:CDS	45.0%
	AGGATTCGAACTTGGAGGAA+TGG	+	chr7.1:3290157-3290176	MS.gene050903:CDS	45.0%
	TCGAACTTGGAGGAATGGAA+TGG	+	chr7.1:3290162-3290181	MS.gene050903:CDS	45.0%
	TCGTAATAGCACTGCATCCA+CGG	-	chr7.1:3290409-3290428	None:intergenic	45.0%
	ACAAAAACAGCCACCTGCGA+AGG	+	chr7.1:3290194-3290213	MS.gene050903:CDS	50.0%
	CGCACCATAGCTGATGTACT+TGG	-	chr7.1:3290303-3290322	None:intergenic	50.0%
	TCTCCAACAGTAGATGCAGC+TGG	+	chr7.1:3290134-3290153	MS.gene050903:CDS	50.0%
!	GTGGATGCAGTGCTATTACG+AGG	+	chr7.1:3290408-3290427	MS.gene050903:CDS	50.0%
	AAGCACCACGGCGAGAACAA+GGG	-	chr7.1:3290343-3290362	None:intergenic	55.0%
	ATCAGCGATGGAACCTTCGC+AGG	-	chr7.1:3290210-3290229	None:intergenic	55.0%
	CACTGCATCCACGGCTGTAA+GGG	-	chr7.1:3290400-3290419	None:intergenic	55.0%
	CCAACAGTAGATGCAGCTGG+AGG	+	chr7.1:3290137-3290156	MS.gene050903:CDS	55.0%
	CCTCCAGCTGCATCTACTGT+TGG	-	chr7.1:3290140-3290159	None:intergenic	55.0%
	GCAGCTGGAGGATTCGAACT+TGG	+	chr7.1:3290149-3290168	MS.gene050903:CDS	55.0%
	GCTGGAGGATTCGAACTTGG+AGG	+	chr7.1:3290152-3290171	MS.gene050903:CDS	55.0%
	TCAGCTATGGTGCGCTTCAG+AGG	+	chr7.1:3290309-3290328	MS.gene050903:CDS	55.0%
!	GCAGTGCTATTACGAGGTGC+AGG	+	chr7.1:3290414-3290433	MS.gene050903:CDS	55.0%
!!	GTAAGGGTTAGCCTGAGCAC+CGG	-	chr7.1:3290384-3290403	None:intergenic	55.0%
!!	TAAGGGTTAGCCTGAGCACC+GGG	-	chr7.1:3290383-3290402	None:intergenic	55.0%
	ACTGTCCCTTGTTCTCGCCG+TGG	+	chr7.1:3290335-3290354	MS.gene050903:CDS	60.0%
	AGCGATGGAACCTTCGCAGG+TGG	-	chr7.1:3290207-3290226	None:intergenic	60.0%
	CAATTGCAGACCCGGTGCTC+AGG	+	chr7.1:3290370-3290389	MS.gene050903:CDS	60.0%
	CAGGCTAACCCTTACAGCCG+TGG	+	chr7.1:3290389-3290408	MS.gene050903:CDS	60.0%
	GCACTGCATCCACGGCTGTA+AGG	-	chr7.1:3290401-3290420	None:intergenic	60.0%
!	GAAGCACCACGGCGAGAACA+AGG	-	chr7.1:3290344-3290363	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.1	gene	3290113	3290442	3290113	ID=MS.gene050903
chr7.1	mRNA	3290113	3290442	3290113	ID=MS.gene050903.t1;Parent=MS.gene050903
chr7.1	exon	3290113	3290442	3290113	ID=MS.gene050903.t1.exon1;Parent=MS.gene050903.t1
chr7.1	CDS	3290113	3290442	3290113	ID=cds.MS.gene050903.t1;Parent=MS.gene050903.t1

Gene Sequence	>MS.gene050903 ATGACAACGATGATGAGTTCTTCTCCAACAGTAGATGCAGCTGGAGGATTCGAACTTGGAGGAATGGAATGGATTCATCAAACAAAAACAGCCACCTGCGAAGGTTCCATCGCTGATTGCATGTTACAACAAGGTGAAGAAGAGTTTCAGTTTGATAATGAAATCAACAGGCGTATCTTAGCAACGACCAAGTACATCAGCTATGGTGCGCTTCAGAGGAACACTGTCCCTTGTTCTCGCCGTGGTGCTTCTTACTACAATTGCAGACCCGGTGCTCAGGCTAACCCTTACAGCCGTGGATGCAGTGCTATTACGAGGTGCAGGAGTTAA
Protein sequence	>MS.gene050903.t1 MTTMMSSSPTVDAAGGFELGGMEWIHQTKTATCEGSIADCMLQQGEEEFQFDNEINRRILATTKYISYGALQRNTVPCSRRGASYYNCRPGAQANPYSRGCSAITRCRS