AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene072588

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072588.t1 MTR_7g111600 93.061 245 16 1 1 245 1 244 2.03e-138 390
MS.gene072588.t1 MTR_7g111600 93.061 245 16 1 1 245 1 244 4.81e-138 391
MS.gene072588.t1 MTR_7g111600 93.061 245 16 1 1 245 1 244 6.44e-138 390
MS.gene072588.t1 MTR_1g084820 56.388 227 64 10 42 240 279 498 2.71e-50 173
MS.gene072588.t1 MTR_1g084820 56.388 227 64 10 42 240 279 498 1.71e-49 172
MS.gene072588.t1 MTR_5g091650 62.727 110 39 1 137 246 86 193 1.89e-38 133
MS.gene072588.t1 MTR_5g091650 62.500 112 40 1 137 248 86 195 3.89e-38 135
MS.gene072588.t1 MTR_3g048280 60.185 108 40 1 137 244 91 195 7.82e-37 129
MS.gene072588.t1 MTR_2g049670 62.069 87 32 1 161 247 158 243 6.08e-32 120
MS.gene072588.t1 MTR_8g089400 48.113 106 50 2 146 246 18 123 2.54e-28 106
MS.gene072588.t1 MTR_8g089400 46.903 113 52 3 139 246 34 143 4.71e-28 106
MS.gene072588.t1 MTR_1g056640 40.667 150 80 4 102 246 347 492 7.38e-27 108
MS.gene072588.t1 MTR_8g085390 51.579 95 44 2 160 253 29 122 7.30e-26 100
MS.gene072588.t1 MTR_5g015720 41.667 144 72 4 105 244 279 414 1.23e-25 104
MS.gene072588.t1 MTR_4g132280 51.613 93 44 1 154 245 375 467 4.76e-25 103
MS.gene072588.t1 MTR_4g079530 53.333 90 41 1 161 249 37 126 1.00e-24 95.9
MS.gene072588.t1 MTR_8g085720 44.444 117 64 1 136 251 339 455 1.24e-24 102
MS.gene072588.t1 MTR_4g079530 54.023 87 39 1 161 246 37 123 2.31e-24 96.3
MS.gene072588.t1 MTR_8g088120 55.556 81 36 0 168 248 55 135 4.03e-24 97.1
MS.gene072588.t1 MTR_5g085580 47.872 94 48 1 154 246 375 468 1.53e-23 99.4
MS.gene072588.t1 MTR_8g085440 50.562 89 38 3 160 245 31 116 3.14e-22 89.0
MS.gene072588.t1 MTR_5g097420 49.412 85 42 1 161 244 30 114 1.35e-21 87.4
MS.gene072588.t1 MTR_5g078200 42.056 107 61 1 154 259 320 426 1.71e-21 94.0
MS.gene072588.t1 MTR_4g133735 41.414 99 57 1 148 245 15 113 1.73e-21 87.0
MS.gene072588.t1 MTR_5g078200 42.056 107 61 1 154 259 353 459 1.86e-21 94.0
MS.gene072588.t1 MTR_3g065460 43.299 97 54 1 154 249 354 450 4.14e-21 92.4
MS.gene072588.t1 MTR_1g022160 47.706 109 46 5 147 252 379 479 8.11e-21 91.7
MS.gene072588.t1 MTR_8g085400 55.696 79 32 2 160 238 32 107 1.71e-20 84.3
MS.gene072588.t1 MTR_3g083580 40.541 111 62 2 148 254 349 459 1.91e-20 90.5
MS.gene072588.t1 MTR_4g069940 43.860 114 54 3 143 248 352 463 2.03e-20 90.5
MS.gene072588.t1 MTR_4g083500 47.778 90 45 2 160 248 401 489 2.79e-20 90.1
MS.gene072588.t1 MTR_5g044530 47.368 95 46 3 156 247 17 110 5.46e-20 86.7
MS.gene072588.t1 MTR_8g085420 51.190 84 35 3 160 240 31 111 1.08e-19 83.2
MS.gene072588.t1 MTR_1g022160 48.515 101 44 4 147 244 379 474 1.15e-19 88.2
MS.gene072588.t1 MTR_1g031900 43.269 104 53 2 160 262 360 458 2.18e-19 87.4
MS.gene072588.t1 MTR_4g133730 41.379 87 50 1 160 245 27 113 4.46e-19 80.9
MS.gene072588.t1 MTR_3g082080 46.250 80 41 2 161 239 157 235 1.79e-18 82.4
MS.gene072588.t1 MTR_6g088885 40.541 111 61 4 136 244 347 454 2.60e-18 84.3
MS.gene072588.t1 MTR_4g083070 47.253 91 45 3 161 249 21 110 2.65e-18 80.9
MS.gene072588.t1 MTR_1g031900 43.478 92 51 1 154 244 354 445 3.05e-18 84.3
MS.gene072588.t1 MTR_1g022160 47.917 96 42 4 147 239 379 469 3.35e-18 84.0
MS.gene072588.t1 MTR_1g116870 44.706 85 45 2 161 244 276 359 4.32e-18 83.2
MS.gene072588.t1 MTR_4g109490 44.944 89 46 3 157 244 20 106 7.00e-18 80.1
MS.gene072588.t1 MTR_2g072500 44.186 86 45 2 160 244 7 90 1.02e-16 73.6
MS.gene072588.t1 MTR_8g102340 34.091 132 76 3 136 261 314 440 1.69e-16 79.0
MS.gene072588.t1 MTR_7g053500 38.983 118 67 3 133 248 271 385 2.00e-16 78.6
MS.gene072588.t1 MTR_3g116510 39.815 108 59 3 144 248 220 324 2.85e-16 77.4
MS.gene072588.t1 MTR_7g053500 38.983 118 67 3 133 248 348 462 3.19e-16 78.2
MS.gene072588.t1 MTR_3g116510 39.815 108 59 3 144 248 354 458 3.35e-16 78.2
MS.gene072588.t1 MTR_8g012400 36.082 97 61 1 152 247 285 381 3.64e-16 77.8
MS.gene072588.t1 MTR_8g012400 36.082 97 61 1 152 247 365 461 3.89e-16 77.8
MS.gene072588.t1 MTR_4g088155 38.043 92 56 1 157 247 369 460 1.15e-14 73.6
MS.gene072588.t1 MTR_1g053320 40.816 98 53 4 160 255 373 467 7.86e-14 71.2
MS.gene072588.t1 MTR_7g026340 41.379 87 45 3 159 242 363 446 1.79e-13 70.1
MS.gene072588.t1 MTR_3g095050 35.714 112 62 5 141 245 348 456 6.31e-13 68.6
MS.gene072588.t1 MTR_4g112025 36.275 102 60 4 160 259 373 471 2.54e-12 66.6
MS.gene072588.t1 MTR_3g031770 40.698 86 47 3 161 245 364 446 3.05e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072588.t1 AT1G09460 59.375 128 48 2 121 248 99 222 8.45e-38 135
MS.gene072588.t1 AT2G30933 61.798 89 34 0 160 248 81 169 3.62e-34 123
MS.gene072588.t1 AT1G29380 58.889 90 37 0 160 249 147 236 4.39e-33 121
MS.gene072588.t1 AT1G29380 58.824 85 35 0 160 244 147 231 1.33e-31 119
MS.gene072588.t1 AT2G30933 62.025 79 30 0 160 238 81 159 1.04e-30 112
MS.gene072588.t1 AT2G30933 62.025 79 30 0 160 238 81 159 1.04e-30 112
MS.gene072588.t1 AT1G29380 58.750 80 33 0 160 239 147 226 2.08e-29 111
MS.gene072588.t1 AT1G29380 58.750 80 33 0 160 239 147 226 2.08e-29 111
MS.gene072588.t1 AT4G05430 45.133 113 57 2 141 248 24 136 2.28e-27 103
MS.gene072588.t1 AT1G11820 50.476 105 51 1 148 251 368 472 3.78e-27 109
MS.gene072588.t1 AT4G05430 45.045 111 57 2 142 248 1 111 4.10e-27 102
MS.gene072588.t1 AT3G58100 50.000 94 46 1 161 253 41 134 1.17e-25 100
MS.gene072588.t1 AT4G05430 47.115 104 50 2 141 239 24 127 1.59e-25 98.2
MS.gene072588.t1 AT2G01630 44.340 106 58 1 154 258 353 458 2.79e-25 104
MS.gene072588.t1 AT3G58100 50.000 94 46 1 161 253 117 210 4.44e-25 100
MS.gene072588.t1 AT4G26830 43.590 117 53 2 131 246 349 453 4.95e-25 103
MS.gene072588.t1 AT4G26830 43.590 117 53 2 131 246 353 457 5.46e-25 103
MS.gene072588.t1 AT5G55180 52.222 90 42 1 158 246 369 458 1.08e-24 102
MS.gene072588.t1 AT2G05790 33.750 240 138 8 23 246 236 470 1.48e-24 102
MS.gene072588.t1 AT5G35740 47.368 95 46 3 154 245 23 116 4.73e-24 94.0
MS.gene072588.t1 AT1G66250 44.554 101 51 2 154 251 361 459 9.55e-23 97.4
MS.gene072588.t1 AT1G11820 50.538 93 45 1 148 239 368 460 1.59e-22 96.7
MS.gene072588.t1 AT1G18650 46.237 93 48 2 157 248 17 108 1.75e-22 91.7
MS.gene072588.t1 AT1G18650 46.237 93 48 2 157 248 17 108 1.99e-22 90.1
MS.gene072588.t1 AT4G13600 47.115 104 52 3 161 263 61 162 4.02e-22 92.0
MS.gene072588.t1 AT5G55180 53.012 83 38 1 158 239 369 451 7.16e-22 94.7
MS.gene072588.t1 AT5G67460 38.571 140 70 5 113 244 246 377 1.58e-21 92.8
MS.gene072588.t1 AT4G34480 47.674 86 43 2 161 245 253 337 3.67e-21 92.0
MS.gene072588.t1 AT4G34480 47.674 86 43 2 161 245 364 448 5.11e-21 92.4
MS.gene072588.t1 AT2G01630 46.512 86 45 1 154 238 353 438 1.73e-20 90.5
MS.gene072588.t1 AT5G67460 38.806 134 66 5 113 238 246 371 5.33e-20 88.6
MS.gene072588.t1 AT5G24318 41.121 107 57 3 141 246 217 318 5.82e-20 87.8
MS.gene072588.t1 AT3G55430 42.553 94 52 2 152 244 357 449 8.75e-20 88.6
MS.gene072588.t1 AT5G24318 41.121 107 57 3 141 246 356 457 9.42e-20 88.6
MS.gene072588.t1 AT2G16230 45.055 91 48 2 161 250 366 455 9.43e-20 88.6
MS.gene072588.t1 AT5G63240 38.393 112 62 4 139 246 20 128 5.97e-19 80.9
MS.gene072588.t1 AT5G63250 40.816 98 53 3 153 246 34 130 8.54e-19 80.5
MS.gene072588.t1 AT1G66870 38.298 94 55 2 153 244 18 110 1.92e-18 79.0
MS.gene072588.t1 AT1G66870 37.895 95 56 2 152 244 44 137 2.78e-18 79.3
MS.gene072588.t1 AT2G04910 40.860 93 54 1 154 245 8 100 2.93e-18 78.2
MS.gene072588.t1 AT1G79480 44.706 85 45 2 161 244 311 394 3.53e-18 83.6
MS.gene072588.t1 AT1G79480 44.706 85 45 2 161 244 310 393 3.58e-18 83.6
MS.gene072588.t1 AT5G24318 42.424 99 51 3 141 238 356 449 5.63e-18 83.2
MS.gene072588.t1 AT1G66852 34.783 92 60 0 153 244 18 109 9.15e-18 77.0
MS.gene072588.t1 AT5G63225 41.860 86 48 2 160 244 24 108 9.54e-18 77.0
MS.gene072588.t1 AT2G43660 41.758 91 52 1 159 248 33 123 1.79e-17 76.6
MS.gene072588.t1 AT2G43660 41.758 91 52 1 159 248 32 122 1.91e-17 76.6
MS.gene072588.t1 AT4G16165 38.710 93 56 1 153 244 18 110 2.42e-17 75.9
MS.gene072588.t1 AT2G39640 35.246 122 68 3 136 247 426 546 3.59e-17 81.3
MS.gene072588.t1 AT2G39640 40.909 88 50 2 156 242 354 440 8.49e-15 74.3
MS.gene072588.t1 AT2G43670 40.426 94 53 2 153 245 27 118 4.55e-17 75.5
MS.gene072588.t1 AT5G56590 38.000 100 61 1 161 259 369 468 7.74e-17 80.1
MS.gene072588.t1 AT4G09465 40.217 92 53 2 156 246 26 116 9.00e-17 74.7
MS.gene072588.t1 AT1G69295 45.349 86 44 3 157 241 17 100 1.03e-16 77.0
MS.gene072588.t1 AT4G09467 40.217 92 53 2 156 246 26 116 1.06e-16 74.3
MS.gene072588.t1 AT4G09464 40.217 92 53 2 156 246 26 116 1.06e-16 74.3
MS.gene072588.t1 AT4G09462 39.000 100 59 2 148 246 16 114 1.09e-16 74.3
MS.gene072588.t1 AT1G66855 39.535 86 51 1 160 244 25 110 1.22e-16 74.3
MS.gene072588.t1 AT3G23770 39.048 105 60 2 145 248 374 475 1.43e-16 79.3
MS.gene072588.t1 AT5G08000 48.387 93 46 2 154 245 33 124 1.56e-16 76.6
MS.gene072588.t1 AT2G04910 41.379 87 50 1 154 239 8 94 2.35e-16 73.9
MS.gene072588.t1 AT4G09466 40.217 92 53 2 156 246 26 116 2.51e-16 73.6
MS.gene072588.t1 AT1G69295 45.349 86 44 3 157 241 13 96 3.26e-16 75.9
MS.gene072588.t1 AT4G09090 42.529 87 48 2 161 246 31 116 4.00e-16 72.8
MS.gene072588.t1 AT5G61130 43.333 90 49 2 157 245 17 105 4.46e-16 75.1
MS.gene072588.t1 AT3G13560 40.625 96 56 1 161 255 362 457 8.53e-16 77.0
MS.gene072588.t1 AT3G13560 40.625 96 56 1 161 255 362 457 8.53e-16 77.0
MS.gene072588.t1 AT3G13560 40.625 96 56 1 161 255 362 457 8.53e-16 77.0
MS.gene072588.t1 AT3G13560 40.625 96 56 1 161 255 362 457 8.53e-16 77.0
MS.gene072588.t1 AT3G13560 40.625 96 56 1 161 255 362 457 8.53e-16 77.0
MS.gene072588.t1 AT4G29360 38.202 89 54 1 160 247 400 488 9.70e-16 77.0
MS.gene072588.t1 AT5G53600 39.785 93 53 2 153 244 21 111 1.02e-15 71.6
MS.gene072588.t1 AT4G29360 38.202 89 54 1 160 247 390 478 1.03e-15 77.0
MS.gene072588.t1 AT5G08000 48.276 87 43 2 160 245 20 105 1.49e-15 73.6
MS.gene072588.t1 AT1G78520 42.529 87 47 2 160 245 31 115 6.14e-15 69.7
MS.gene072588.t1 AT5G63230 37.209 86 52 2 160 244 25 109 6.62e-15 69.3
MS.gene072588.t1 AT2G42930 39.241 79 47 1 161 239 40 117 6.93e-15 70.1
MS.gene072588.t1 AT1G26450 45.455 77 39 3 157 232 17 91 1.30e-14 70.9
MS.gene072588.t1 AT4G14080 35.897 117 67 3 137 248 364 477 1.45e-14 73.6
MS.gene072588.t1 AT2G03505 36.458 96 57 3 157 249 17 111 1.87e-14 68.6
MS.gene072588.t1 AT2G03505 36.170 94 56 3 157 247 17 109 2.57e-14 69.3
MS.gene072588.t1 AT2G03505 36.170 94 56 3 157 247 17 109 2.57e-14 69.3
MS.gene072588.t1 AT2G03505 36.170 94 56 3 157 247 17 109 2.57e-14 69.3
MS.gene072588.t1 AT5G53610 41.176 85 47 2 161 244 28 110 7.15e-14 66.6
MS.gene072588.t1 AT3G07320 39.785 93 55 1 157 248 367 459 7.16e-14 71.2
MS.gene072588.t1 AT1G13830 43.373 83 40 4 158 238 32 109 7.37e-13 66.2
MS.gene072588.t1 AT1G13830 43.750 80 38 4 161 238 21 95 1.36e-12 65.5
MS.gene072588.t1 AT3G04010 40.860 93 49 4 160 249 373 462 4.81e-12 65.9
MS.gene072588.t1 AT4G17180 38.372 86 49 3 160 244 358 440 4.86e-12 65.9
MS.gene072588.t1 AT5G58090 41.573 89 46 4 160 245 361 446 8.05e-12 65.1
MS.gene072588.t1 AT2G29735 35.870 92 55 3 156 244 26 116 7.50e-11 58.5

Find 75 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTGGATTTGTTGGTGATAA+TGG 0.267994 7.4:+4794158 None:intergenic
AGTCCTCCATCTTCTTCTTC+AGG 0.320635 7.4:-4794002 MS.gene072588:CDS
TGCATCTTCCCATCAGCTTC+AGG 0.344512 7.4:-4793627 MS.gene072588:CDS
TTGTAACAGATCACAAGATT+TGG 0.357056 7.4:-4794313 MS.gene072588:CDS
AGTGTAGATTGTGGTGTTCC+AGG 0.362624 7.4:+4793957 None:intergenic
CCACCACCATCACCATTATC+AGG 0.376324 7.4:-4794071 MS.gene072588:CDS
GTTCCTGTTCCTCCTGATAA+TGG 0.379708 7.4:+4794059 None:intergenic
GGTGAATTTGCTGGATTTGT+TGG 0.382630 7.4:+4794149 None:intergenic
GCTGCAACAAGCTGTGACTT+TGG 0.388632 7.4:-4793795 MS.gene072588:CDS
GGCACAGGCACAGGCACATC+CGG 0.389361 7.4:-4793588 MS.gene072588:CDS
GGAACTGTTGGTGGTGTAAC+AGG 0.393025 7.4:+4794122 None:intergenic
GGAGGAGCTGGCGCAGGCAC+AGG 0.414477 7.4:-4793603 MS.gene072588:CDS
CTTCTTCTTCAGGAGGTAGC+TGG 0.414888 7.4:-4793992 MS.gene072588:CDS
GCTGGCGCAGGCACAGGCAC+AGG 0.415709 7.4:-4793597 MS.gene072588:CDS
GACTATGCTTGTGGAACAAA+AGG 0.417962 7.4:-4793921 MS.gene072588:CDS
TTCACTGGTGTTGTTCCTCC+TGG 0.427069 7.4:+4794101 None:intergenic
GATGCAAGTAGAAGCTTCAT+TGG 0.437577 7.4:+4794546 None:intergenic
GTTACACCACCAACAGTTCC+AGG 0.439114 7.4:-4794119 MS.gene072588:CDS
ACAGGTACTGGTGAATTTGC+TGG 0.439151 7.4:+4794140 None:intergenic
AAGTCACAGCTTGTTGCAGC+AGG 0.460540 7.4:+4793798 None:intergenic
GTTGTTCCTCCTGGAACTGT+TGG 0.460619 7.4:+4794110 None:intergenic
TCAGCTTCAGGAGGAGGAGC+TGG 0.463929 7.4:-4793615 MS.gene072588:CDS
GGTGTTGCACCTGGATTTGT+TGG 0.466876 7.4:+4794215 None:intergenic
ACAGCTGGTGGAGTAGTAGA+AGG 0.473175 7.4:+4794182 None:intergenic
AGAAGAAAATGAGAAAAGAT+AGG 0.477075 7.4:+4794518 None:intergenic
CAGTGATGATTGTTGGTGAT+GGG 0.480237 7.4:+4794270 None:intergenic
AGCTGGTGTGTGGCTAAACC+TGG 0.480971 7.4:-4793975 MS.gene072588:CDS
GTTCCTCCTGGAACTGTTGG+TGG 0.482015 7.4:+4794113 None:intergenic
TCACCATTATCAGGAGGAAC+AGG 0.487525 7.4:-4794062 MS.gene072588:CDS
TTCCCATCAGCTTCAGGAGG+AGG 0.492744 7.4:-4793621 MS.gene072588:CDS
TCAGGAGGAGGAGCTGGCGC+AGG 0.502820 7.4:-4793609 MS.gene072588:CDS
CAACAAGCTGTGACTTTGGA+GGG 0.506706 7.4:-4793791 MS.gene072588:CDS
ATCTACACTTCAAACAGCAT+TGG 0.509235 7.4:-4793943 MS.gene072588:CDS
AGAAATAGTTGAAAATGCAT+TGG 0.517953 7.4:+4794388 None:intergenic
GATGGAGGACTTGTGCTTGA+AGG 0.519243 7.4:+4794014 None:intergenic
GAAGGAACAGTGATGATTGT+TGG 0.527796 7.4:+4794263 None:intergenic
GCAACAAGCTGTGACTTTGG+AGG 0.530607 7.4:-4793792 MS.gene072588:CDS
GAAGGTGGAGGATTTGTGAC+AGG 0.530909 7.4:+4794032 None:intergenic
GTTGGAGAAACTGTTGTGAC+AGG 0.533113 7.4:+4794233 None:intergenic
CCTGATAATGGTGATGGTGG+TGG 0.538366 7.4:+4794071 None:intergenic
CTCCTCCTCCTGAAGCTGAT+GGG 0.539809 7.4:+4793619 None:intergenic
TGAAGCTGATGGGAAGATGC+AGG 0.543799 7.4:+4793629 None:intergenic
CTACCTCCTGAAGAAGAAGA+TGG 0.552545 7.4:+4793996 None:intergenic
ACAGTGATGATTGTTGGTGA+TGG 0.553424 7.4:+4794269 None:intergenic
GTTCCTCCTGATAATGGTGA+TGG 0.553903 7.4:+4794065 None:intergenic
GCTCCTCCTCCTGAAGCTGA+TGG 0.558816 7.4:+4793618 None:intergenic
GGACTTGTGCTTGAAGGTGG+AGG 0.559600 7.4:+4794020 None:intergenic
GTTGGTGATAATGGAACAGC+TGG 0.559840 7.4:+4794167 None:intergenic
GTAGTAGAAGGAACTGTGAC+AGG 0.561662 7.4:+4794194 None:intergenic
CACGGTTCTCACTTACCCGC+CGG 0.563441 7.4:+4793569 None:intergenic
CAGGCACAGGCACATCCGGC+GGG 0.564968 7.4:-4793584 MS.gene072588:CDS
ACAGTTTCTCCAACAAATCC+AGG 0.567466 7.4:-4794224 MS.gene072588:CDS
TTCAGGAGGTAGCTGGTGTG+TGG 0.575773 7.4:-4793985 MS.gene072588:CDS
GTTGGTGGTGTAACAGGTAC+TGG 0.587227 7.4:+4794128 None:intergenic
ACTGTGACAGGTGTTGCACC+TGG 0.594459 7.4:+4794206 None:intergenic
GTTGTGACAGGATTGGTTGA+AGG 0.600451 7.4:+4794245 None:intergenic
ACAGCATTGGACTATGCTTG+TGG 0.604056 7.4:-4793930 MS.gene072588:CDS
CCTCCATCTTCTTCTTCAGG+AGG 0.605110 7.4:-4793999 MS.gene072588:CDS
AACAAGCTGTGACTTTGGAG+GGG 0.609446 7.4:-4793790 MS.gene072588:CDS
AGAAACTGTTGTGACAGGAT+TGG 0.611206 7.4:+4794238 None:intergenic
CCTCCTGATAATGGTGATGG+TGG 0.614026 7.4:+4794068 None:intergenic
TGTTGAATGCAAAAGAAGCA+TGG 0.614920 7.4:+4793838 None:intergenic
GATTGTAACAGCTACCACCT+TGG 0.617114 7.4:+4793877 None:intergenic
ACAGGCACAGGCACATCCGG+CGG 0.622496 7.4:-4793585 MS.gene072588:CDS
GGAGGACTTGTGCTTGAAGG+TGG 0.626099 7.4:+4794017 None:intergenic
ATCTTCCCATCAGCTTCAGG+AGG 0.632762 7.4:-4793624 MS.gene072588:CDS
GGTGATAATGGAACAGCTGG+TGG 0.636040 7.4:+4794170 None:intergenic
GGTGGTGGATAAGAATTCAC+TGG 0.638656 7.4:+4794086 None:intergenic
ACTAGGATTAGTGCTAGTAA+TGG 0.644779 7.4:+4793761 None:intergenic
GATTGTTCACAAATAAACCA+AGG 0.679441 7.4:-4793894 MS.gene072588:CDS
CCTCCTGAAGAAGAAGATGG+AGG 0.682773 7.4:+4793999 None:intergenic
CCACCATCACCATTATCAGG+AGG 0.700034 7.4:-4794068 MS.gene072588:CDS
TGTTCACAAATAAACCAAGG+TGG 0.741309 7.4:-4793891 MS.gene072588:CDS
GCTGTTTGAAGTGTAGATTG+TGG 0.741949 7.4:+4793948 None:intergenic
ACACCACCAACAGTTCCAGG+AGG 0.766003 7.4:-4794116 MS.gene072588:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATAGATTAGATCTTACT+AGG + chr7.4:4794390-4794409 None:intergenic 20.0%
!! CAAGCATAATATGAAAAATT+AGG + chr7.4:4794423-4794442 None:intergenic 20.0%
!! TGTAAAAATAGCATTAAACA+AGG + chr7.4:4793688-4793707 None:intergenic 20.0%
! AGAAATAGTTGAAAATGCAT+TGG + chr7.4:4793746-4793765 None:intergenic 25.0%
! AGAAGAAAATGAGAAAAGAT+AGG + chr7.4:4793616-4793635 None:intergenic 25.0%
!! AATGCATTTTCAACTATTTC+TGG - chr7.4:4793745-4793764 MS.gene072588:intron 25.0%
GATTGTTCACAAATAAACCA+AGG - chr7.4:4794237-4794256 MS.gene072588:CDS 30.0%
TTGTAACAGATCACAAGATT+TGG - chr7.4:4793818-4793837 MS.gene072588:CDS 30.0%
! TTTCTCATTTTCTTCTGTTC+TGG - chr7.4:4793620-4793639 MS.gene072588:CDS 30.0%
!! GCTACTTTCAAATTCTTTTC+AGG - chr7.4:4793718-4793737 MS.gene072588:intron 30.0%
ATCTACACTTCAAACAGCAT+TGG - chr7.4:4794188-4794207 MS.gene072588:CDS 35.0%
TGTTCACAAATAAACCAAGG+TGG - chr7.4:4794240-4794259 MS.gene072588:CDS 35.0%
TGTTGAATGCAAAAGAAGCA+TGG + chr7.4:4794296-4794315 None:intergenic 35.0%
TTTGAATTGTGACTAGGTTC+TGG - chr7.4:4794477-4794496 MS.gene072588:intron 35.0%
! ACTAGGATTAGTGCTAGTAA+TGG + chr7.4:4794373-4794392 None:intergenic 35.0%
! CTCTCTTTTGAATTGTGACT+AGG - chr7.4:4794471-4794490 MS.gene072588:intron 35.0%
!!! GACTTTGAGATCTTGCTTTT+TGG + chr7.4:4793784-4793803 None:intergenic 35.0%
!!! GCATGGTTTTGAAGTGAATT+TGG + chr7.4:4794279-4794298 None:intergenic 35.0%
!!! TTGAGATCTTGCTTTTTGGA+AGG + chr7.4:4793780-4793799 None:intergenic 35.0%
ACAGTTTCTCCAACAAATCC+AGG - chr7.4:4793907-4793926 MS.gene072588:CDS 40.0%
AGAAACTGTTGTGACAGGAT+TGG + chr7.4:4793896-4793915 None:intergenic 40.0%
GAAGGAACAGTGATGATTGT+TGG + chr7.4:4793871-4793890 None:intergenic 40.0%
GACTATGCTTGTGGAACAAA+AGG - chr7.4:4794210-4794229 MS.gene072588:CDS 40.0%
GATGCAAGTAGAAGCTTCAT+TGG + chr7.4:4793588-4793607 None:intergenic 40.0%
! ACAGTGATGATTGTTGGTGA+TGG + chr7.4:4793865-4793884 None:intergenic 40.0%
! GCTGTTTGAAGTGTAGATTG+TGG + chr7.4:4794186-4794205 None:intergenic 40.0%
!! CAGTGATGATTGTTGGTGAT+GGG + chr7.4:4793864-4793883 None:intergenic 40.0%
!! GCTGGATTTGTTGGTGATAA+TGG + chr7.4:4793976-4793995 None:intergenic 40.0%
!! GGTGAATTTGCTGGATTTGT+TGG + chr7.4:4793985-4794004 None:intergenic 40.0%
AGTCCTCCATCTTCTTCTTC+AGG - chr7.4:4794129-4794148 MS.gene072588:CDS 45.0%
AGTGTAGATTGTGGTGTTCC+AGG + chr7.4:4794177-4794196 None:intergenic 45.0%
CAACAAGCTGTGACTTTGGA+GGG - chr7.4:4794340-4794359 MS.gene072588:CDS 45.0%
CTACCTCCTGAAGAAGAAGA+TGG + chr7.4:4794138-4794157 None:intergenic 45.0%
GATTGTAACAGCTACCACCT+TGG + chr7.4:4794257-4794276 None:intergenic 45.0%
GTAGTAGAAGGAACTGTGAC+AGG + chr7.4:4793940-4793959 None:intergenic 45.0%
GTTCCTGTTCCTCCTGATAA+TGG + chr7.4:4794075-4794094 None:intergenic 45.0%
GTTGGAGAAACTGTTGTGAC+AGG + chr7.4:4793901-4793920 None:intergenic 45.0%
TCACCATTATCAGGAGGAAC+AGG - chr7.4:4794069-4794088 MS.gene072588:CDS 45.0%
! AACAAGCTGTGACTTTGGAG+GGG - chr7.4:4794341-4794360 MS.gene072588:CDS 45.0%
! ACAGCATTGGACTATGCTTG+TGG - chr7.4:4794201-4794220 MS.gene072588:CDS 45.0%
!! ACAGGTACTGGTGAATTTGC+TGG + chr7.4:4793994-4794013 None:intergenic 45.0%
!! GGTGGTGGATAAGAATTCAC+TGG + chr7.4:4794048-4794067 None:intergenic 45.0%
!! GTTCCTCCTGATAATGGTGA+TGG + chr7.4:4794069-4794088 None:intergenic 45.0%
!! GTTGGTGATAATGGAACAGC+TGG + chr7.4:4793967-4793986 None:intergenic 45.0%
!! GTTGTGACAGGATTGGTTGA+AGG + chr7.4:4793889-4793908 None:intergenic 45.0%
ACAGCTGGTGGAGTAGTAGA+AGG + chr7.4:4793952-4793971 None:intergenic 50.0%
ATCTTCCCATCAGCTTCAGG+AGG - chr7.4:4794507-4794526 MS.gene072588:intron 50.0%
CCACCACCATCACCATTATC+AGG - chr7.4:4794060-4794079 MS.gene072588:CDS 50.0%
CCACCATCACCATTATCAGG+AGG - chr7.4:4794063-4794082 MS.gene072588:CDS 50.0%
CCTCCATCTTCTTCTTCAGG+AGG - chr7.4:4794132-4794151 MS.gene072588:CDS 50.0%
CCTCCTGAAGAAGAAGATGG+AGG + chr7.4:4794135-4794154 None:intergenic 50.0%
CTTCTTCTTCAGGAGGTAGC+TGG - chr7.4:4794139-4794158 MS.gene072588:CDS 50.0%
GAAGGTGGAGGATTTGTGAC+AGG + chr7.4:4794102-4794121 None:intergenic 50.0%
GATGGAGGACTTGTGCTTGA+AGG + chr7.4:4794120-4794139 None:intergenic 50.0%
GCAACAAGCTGTGACTTTGG+AGG - chr7.4:4794339-4794358 MS.gene072588:CDS 50.0%
GCTGCAACAAGCTGTGACTT+TGG - chr7.4:4794336-4794355 MS.gene072588:CDS 50.0%
GTTACACCACCAACAGTTCC+AGG - chr7.4:4794012-4794031 MS.gene072588:CDS 50.0%
GTTGTTCCTCCTGGAACTGT+TGG + chr7.4:4794024-4794043 None:intergenic 50.0%
TGAAGCTGATGGGAAGATGC+AGG + chr7.4:4794505-4794524 None:intergenic 50.0%
TGCATCTTCCCATCAGCTTC+AGG - chr7.4:4794504-4794523 MS.gene072588:intron 50.0%
! GGTGTTGCACCTGGATTTGT+TGG + chr7.4:4793919-4793938 None:intergenic 50.0%
!! AAGTCACAGCTTGTTGCAGC+AGG + chr7.4:4794336-4794355 None:intergenic 50.0%
!! CCTCCTGATAATGGTGATGG+TGG + chr7.4:4794066-4794085 None:intergenic 50.0%
!! CCTGATAATGGTGATGGTGG+TGG + chr7.4:4794063-4794082 None:intergenic 50.0%
!! GGAACTGTTGGTGGTGTAAC+AGG + chr7.4:4794012-4794031 None:intergenic 50.0%
!! GGTGATAATGGAACAGCTGG+TGG + chr7.4:4793964-4793983 None:intergenic 50.0%
!! GTTGGTGGTGTAACAGGTAC+TGG + chr7.4:4794006-4794025 None:intergenic 50.0%
!! TTCACTGGTGTTGTTCCTCC+TGG + chr7.4:4794033-4794052 None:intergenic 50.0%
ACACCACCAACAGTTCCAGG+AGG - chr7.4:4794015-4794034 MS.gene072588:CDS 55.0%
AGCTGGTGTGTGGCTAAACC+TGG - chr7.4:4794156-4794175 MS.gene072588:CDS 55.0%
CTCCTCCTCCTGAAGCTGAT+GGG + chr7.4:4794515-4794534 None:intergenic 55.0%
GGACTTGTGCTTGAAGGTGG+AGG + chr7.4:4794114-4794133 None:intergenic 55.0%
GGAGGACTTGTGCTTGAAGG+TGG + chr7.4:4794117-4794136 None:intergenic 55.0%
GTTCCTCCTGGAACTGTTGG+TGG + chr7.4:4794021-4794040 None:intergenic 55.0%
TTCAGGAGGTAGCTGGTGTG+TGG - chr7.4:4794146-4794165 MS.gene072588:CDS 55.0%
TTCCCATCAGCTTCAGGAGG+AGG - chr7.4:4794510-4794529 MS.gene072588:intron 55.0%
! ACTGTGACAGGTGTTGCACC+TGG + chr7.4:4793928-4793947 None:intergenic 55.0%
GCTCCTCCTCCTGAAGCTGA+TGG + chr7.4:4794516-4794535 None:intergenic 60.0%
TCAGCTTCAGGAGGAGGAGC+TGG - chr7.4:4794516-4794535 MS.gene072588:CDS 60.0%
ACAGGCACAGGCACATCCGG+CGG - chr7.4:4794546-4794565 MS.gene072588:CDS 65.0%
GGCACAGGCACAGGCACATC+CGG - chr7.4:4794543-4794562 MS.gene072588:CDS 65.0%
CAGGCACAGGCACATCCGGC+GGG - chr7.4:4794547-4794566 MS.gene072588:CDS 70.0%
TCAGGAGGAGGAGCTGGCGC+AGG - chr7.4:4794522-4794541 MS.gene072588:CDS 70.0%
GCTGGCGCAGGCACAGGCAC+AGG - chr7.4:4794534-4794553 MS.gene072588:CDS 75.0%
GGAGGAGCTGGCGCAGGCAC+AGG - chr7.4:4794528-4794547 MS.gene072588:CDS 75.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 4793581 4794572 4793581 ID=MS.gene072588
chr7.4 mRNA 4793581 4794572 4793581 ID=MS.gene072588.t1;Parent=MS.gene072588
chr7.4 exon 4794512 4794572 4794512 ID=MS.gene072588.t1.exon1;Parent=MS.gene072588.t1
chr7.4 CDS 4794512 4794572 4794512 ID=cds.MS.gene072588.t1;Parent=MS.gene072588.t1
chr7.4 exon 4793763 4794413 4793763 ID=MS.gene072588.t1.exon2;Parent=MS.gene072588.t1
chr7.4 CDS 4793763 4794413 4793763 ID=cds.MS.gene072588.t1;Parent=MS.gene072588.t1
chr7.4 exon 4793581 4793660 4793581 ID=MS.gene072588.t1.exon3;Parent=MS.gene072588.t1
chr7.4 CDS 4793581 4793660 4793581 ID=cds.MS.gene072588.t1;Parent=MS.gene072588.t1
Gene Sequence

>MS.gene072588

ATGGCCAATGAAGCTTCTACTTGCATCTTGTTCCTATCTTTTCTCATTTTCTTCTGTTCTGGTAAGTAATCTAATCACTAATCAATCATTTTTCTTCTTGTGAACCTTGTTTAATGCTATTTTTACACTCTAATAATGCTACTTTCAAATTCTTTTCAGGAACCAATGCATTTTCAACTATTTCTGGAGCAAGATTCCTTCCAAAAAGCAAGATCTCAAAGTCTCAGATTCAGTTTCTTGTAACAGATCACAAGATTTGGAGAAAACTTCTAGACACAAACCCATCACCAACAATCATCACTGTTCCTTCAACCAATCCTGTCACAACAGTTTCTCCAACAAATCCAGGTGCAACACCTGTCACAGTTCCTTCTACTACTCCACCAGCTGTTCCATTATCACCAACAAATCCAGCAAATTCACCAGTACCTGTTACACCACCAACAGTTCCAGGAGGAACAACACCAGTGAATTCTTATCCACCACCATCACCATTATCAGGAGGAACAGGAACTGTTCCTGTCACAAATCCTCCACCTTCAAGCACAAGTCCTCCATCTTCTTCTTCAGGAGGTAGCTGGTGTGTGGCTAAACCTGGAACACCACAATCTACACTTCAAACAGCATTGGACTATGCTTGTGGAACAAAAGGAACTGATTGTTCACAAATAAACCAAGGTGGTAGCTGTTACAATCCAAATTCACTTCAAAACCATGCTTCTTTTGCATTCAACAGCTACTATGTAAAGAATCCTGCTGCAACAAGCTGTGACTTTGGAGGGGTTGCTACCATTACTAGCACTAATCCTAGTAAGATCTAATCTATAATAGTTTTATATCCTAATTTTTCATATTATGCTTGATGAAATTTTAAAACATGTTTTGATGAACTCTCTTTTGAATTGTGACTAGGTTCTGGTACCTGCATCTTCCCATCAGCTTCAGGAGGAGGAGCTGGCGCAGGCACAGGCACAGGCACATCCGGCGGGTAA
Protein sequence

>MS.gene072588.t1

MANEASTCILFLSFLIFFCSGTNAFSTISGARFLPKSKISKSQIQFLVTDHKIWRKLLDTNPSPTIITVPSTNPVTTVSPTNPGATPVTVPSTTPPAVPLSPTNPANSPVPVTPPTVPGGTTPVNSYPPPSPLSGGTGTVPVTNPPPSSTSPPSSSSGGSWCVAKPGTPQSTLQTALDYACGTKGTDCSQINQGGSCYNPNSLQNHASFAFNSYYVKNPAATSCDFGGVATITSTNPSSGTCIFPSASGGGAGAGTGTGTSGG