Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072736.t1 | XP_003611012.1 | 97 | 99 | 3 | 0 | 6 | 104 | 93 | 191 | 3.30E-43 | 184.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072736.t1 | G7KAM4 | 97.0 | 99 | 3 | 0 | 6 | 104 | 93 | 191 | 2.4e-43 | 184.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050759 | MS.gene072736 | 0.805331 | 1.41E-49 | -1.69E-46 |
| MS.gene050962 | MS.gene072736 | 0.801151 | 1.03E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072736.t1 | MTR_5g009490 | 96.154 | 104 | 4 | 0 | 1 | 104 | 88 | 191 | 3.28e-68 | 202 |
| MS.gene072736.t1 | MTR_8g099340 | 46.729 | 107 | 51 | 4 | 2 | 104 | 71 | 175 | 5.31e-18 | 74.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCCAAAGAGTGTTTGTATA+TGG | 0.256621 | 5.3:-3448715 | MS.gene072736:CDS |
| AACCATATACAAACACTCTT+TGG | 0.339260 | 5.3:+3448713 | None:intergenic |
| ATCTCAAATTCAGGAACTTC+AGG | 0.352303 | 5.3:+3448657 | None:intergenic |
| TATATGGTTTATGATAAGTC+TGG | 0.403894 | 5.3:-3448699 | MS.gene072736:CDS |
| AGAACACCAATCTCAAATTC+AGG | 0.405479 | 5.3:+3448648 | None:intergenic |
| CGGTTCACGACCACCACAAT+TGG | 0.418761 | 5.3:-3448582 | MS.gene072736:CDS |
| TTTGAAACTATTGCTGCTGC+TGG | 0.434551 | 5.3:+3448752 | None:intergenic |
| GAAGTTCCTGAATTTGAGAT+TGG | 0.459665 | 5.3:-3448654 | MS.gene072736:CDS |
| TCAGGTATAGTAGAAAGAAC+TGG | 0.526063 | 5.3:+3448675 | None:intergenic |
| TTAACCAAGGAAAATACCTC+AGG | 0.528566 | 5.3:+3448621 | None:intergenic |
| AACGTTTCAAGCAGTATCAC+TGG | 0.562635 | 5.3:-3448777 | MS.gene072736:CDS |
| GACGCACGCAATACCAATTG+TGG | 0.574615 | 5.3:+3448569 | None:intergenic |
| AAAGAACGAGAAATCTTGTA+CGG | 0.579520 | 5.3:+3448846 | None:intergenic |
| TTAAAAGGTCAGCTTCTGAT+CGG | 0.581046 | 5.3:-3448602 | MS.gene072736:CDS |
| GATTGGTGTTCTTTCGCCTG+AGG | 0.584037 | 5.3:-3448637 | MS.gene072736:CDS |
| AGTGATACTGCTTGAAACGT+TGG | 0.596196 | 5.3:+3448779 | None:intergenic |
| GCACGCAATACCAATTGTGG+TGG | 0.661029 | 5.3:+3448572 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TATATGGTTTATGATAAGTC+TGG | - | chr5.3:3448725-3448744 | MS.gene072736:CDS | 25.0% |
| AAAGAACGAGAAATCTTGTA+CGG | + | chr5.3:3448581-3448600 | None:intergenic | 30.0% | |
| AACCATATACAAACACTCTT+TGG | + | chr5.3:3448714-3448733 | None:intergenic | 30.0% | |
| ! | AGGTATTTTCCTTGGTTAAA+AGG | - | chr5.3:3448807-3448826 | MS.gene072736:CDS | 30.0% |
| AGAACACCAATCTCAAATTC+AGG | + | chr5.3:3448779-3448798 | None:intergenic | 35.0% | |
| ATCTCAAATTCAGGAACTTC+AGG | + | chr5.3:3448770-3448789 | None:intergenic | 35.0% | |
| TCAGGTATAGTAGAAAGAAC+TGG | + | chr5.3:3448752-3448771 | None:intergenic | 35.0% | |
| TTAAAAGGTCAGCTTCTGAT+CGG | - | chr5.3:3448822-3448841 | MS.gene072736:CDS | 35.0% | |
| TTAACCAAGGAAAATACCTC+AGG | + | chr5.3:3448806-3448825 | None:intergenic | 35.0% | |
| ! | GAAGTTCCTGAATTTGAGAT+TGG | - | chr5.3:3448770-3448789 | MS.gene072736:CDS | 35.0% |
| !! | CTCCAAAGAGTGTTTGTATA+TGG | - | chr5.3:3448709-3448728 | MS.gene072736:CDS | 35.0% |
| !!! | ATTCGTTCAGTTTTCAAGTC+AGG | - | chr5.3:3448611-3448630 | MS.gene072736:CDS | 35.0% |
| AACGTTTCAAGCAGTATCAC+TGG | - | chr5.3:3448647-3448666 | MS.gene072736:CDS | 40.0% | |
| AGTGATACTGCTTGAAACGT+TGG | + | chr5.3:3448648-3448667 | None:intergenic | 40.0% | |
| ! | AGAAGCTGACCTTTTAACCA+AGG | + | chr5.3:3448819-3448838 | None:intergenic | 40.0% |
| ! | TTTGAAACTATTGCTGCTGC+TGG | + | chr5.3:3448675-3448694 | None:intergenic | 40.0% |
| !! | TTCGCCTGAGGTATTTTCCT+TGG | - | chr5.3:3448799-3448818 | MS.gene072736:CDS | 45.0% |
| GACGCACGCAATACCAATTG+TGG | + | chr5.3:3448858-3448877 | None:intergenic | 50.0% | |
| GCACGCAATACCAATTGTGG+TGG | + | chr5.3:3448855-3448874 | None:intergenic | 50.0% | |
| ! | GATTGGTGTTCTTTCGCCTG+AGG | - | chr5.3:3448787-3448806 | MS.gene072736:CDS | 50.0% |
| CGGTTCACGACCACCACAAT+TGG | - | chr5.3:3448842-3448861 | MS.gene072736:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 3448566 | 3448880 | 3448566 | ID=MS.gene072736 |
| chr5.3 | mRNA | 3448566 | 3448880 | 3448566 | ID=MS.gene072736.t1;Parent=MS.gene072736 |
| chr5.3 | exon | 3448566 | 3448880 | 3448566 | ID=MS.gene072736.t1.exon1;Parent=MS.gene072736.t1 |
| chr5.3 | CDS | 3448566 | 3448880 | 3448566 | ID=cds.MS.gene072736.t1;Parent=MS.gene072736.t1 |
| Gene Sequence |
| Protein sequence |
