AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene072828

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072828.t1 MTR_5g010940 88.312 231 25 2 1 231 1 229 2.27e-150 417
MS.gene072828.t1 MTR_5g010910 85.776 232 24 3 1 231 1 224 8.27e-137 383
MS.gene072828.t1 MTR_5g010930 85.784 204 26 3 30 231 24 226 2.52e-124 352
MS.gene072828.t1 MTR_4g102660 57.870 216 75 7 24 231 13 220 1.49e-58 184
MS.gene072828.t1 MTR_1g101600 47.685 216 93 5 18 231 5 202 1.90e-53 171
MS.gene072828.t1 MTR_6g465420 63.359 131 37 2 16 146 19 138 1.07e-52 171
MS.gene072828.t1 MTR_6g465430 64.062 128 35 2 19 146 22 138 2.54e-52 169
MS.gene072828.t1 MTR_6g465450 62.963 135 39 2 12 146 15 138 1.11e-51 168
MS.gene072828.t1 MTR_6g465460 58.647 133 48 1 29 161 28 153 2.36e-51 168
MS.gene072828.t1 MTR_6g466130 65.833 120 34 1 27 146 38 150 9.51e-51 165
MS.gene072828.t1 MTR_6g466000 61.240 129 43 1 18 146 20 141 4.72e-50 163
MS.gene072828.t1 MTR_6g465690 44.292 219 94 5 14 222 15 215 1.39e-48 160
MS.gene072828.t1 MTR_6g465530 46.190 210 92 5 26 229 4 198 2.57e-48 157
MS.gene072828.t1 MTR_6g465510 64.167 120 36 1 27 146 5 117 9.22e-45 149
MS.gene072828.t1 MTR_6g465850 63.333 120 37 1 27 146 35 147 2.24e-39 137
MS.gene072828.t1 MTR_6g466020 57.895 114 41 1 19 132 7 113 2.35e-39 134
MS.gene072828.t1 MTR_2g085015 61.458 96 30 3 49 143 41 130 1.30e-29 110
MS.gene072828.t1 MTR_5g012910 49.194 124 49 2 51 167 24 140 1.81e-28 106
MS.gene072828.t1 MTR_3g110205 55.208 96 36 1 50 145 38 126 4.35e-27 102
MS.gene072828.t1 MTR_8g006410 54.082 98 38 1 39 136 36 126 2.82e-26 101
MS.gene072828.t1 MTR_6g465990 48.598 107 28 2 30 136 18 97 5.69e-26 100
MS.gene072828.t1 MTR_7g117690 41.481 135 63 3 35 166 14 135 4.53e-25 97.4
MS.gene072828.t1 MTR_8g102800 51.304 115 46 3 39 153 23 127 1.16e-24 97.8
MS.gene072828.t1 MTR_7g046260 53.061 98 39 1 39 136 45 135 1.47e-24 97.8
MS.gene072828.t1 MTR_2g101340 52.525 99 34 2 35 127 15 106 2.06e-24 96.7
MS.gene072828.t1 MTR_8g013860 50.000 106 46 1 51 156 43 141 1.10e-23 94.7
MS.gene072828.t1 MTR_6g088405 56.250 96 35 1 52 147 41 129 2.21e-23 94.0
MS.gene072828.t1 MTR_2g078510 67.606 71 22 1 145 214 1 71 1.94e-22 93.2
MS.gene072828.t1 MTR_4g102670 54.651 86 31 2 51 136 11 88 2.31e-22 90.1
MS.gene072828.t1 MTR_8g027465 50.000 94 37 2 33 126 20 103 9.34e-22 89.0
MS.gene072828.t1 MTR_4g100650 62.319 69 19 1 53 121 72 133 4.52e-21 89.0
MS.gene072828.t1 MTR_6g088425 42.553 141 57 3 14 145 7 132 1.61e-17 79.0
MS.gene072828.t1 MTR_4g035430 40.000 110 57 2 36 143 11 113 2.26e-16 75.5
MS.gene072828.t1 MTR_1g006660 67.164 67 15 1 51 117 43 102 2.64e-16 75.9
MS.gene072828.t1 MTR_5g008590 52.809 89 35 1 55 143 11 92 4.56e-15 71.2
MS.gene072828.t1 MTR_3g102100 52.381 63 23 1 55 117 45 100 1.59e-14 69.3
MS.gene072828.t1 MTR_1g014780 49.254 67 27 1 54 120 37 96 1.76e-14 70.5
MS.gene072828.t1 MTR_1g019110 52.308 65 24 1 55 119 44 101 5.92e-14 68.2
MS.gene072828.t1 MTR_5g008470 42.222 90 45 1 57 146 77 159 6.60e-14 68.2
MS.gene072828.t1 MTR_1g090170 47.059 68 29 1 50 117 147 207 8.86e-14 69.7
MS.gene072828.t1 MTR_5g008550 46.739 92 42 1 55 146 21 105 1.14e-13 67.4
MS.gene072828.t1 MTR_5g083340 53.846 65 23 1 55 119 85 142 1.71e-13 69.3
MS.gene072828.t1 MTR_5g016750 52.381 63 23 1 55 117 25 80 1.89e-13 66.6
MS.gene072828.t1 MTR_1g014820 36.792 106 51 2 21 126 2 91 1.90e-13 68.2
MS.gene072828.t1 MTR_1g090170 47.059 68 29 1 50 117 234 294 2.05e-13 68.9
MS.gene072828.t1 MTR_3g074130 46.835 79 33 2 39 117 136 205 2.39e-13 68.6
MS.gene072828.t1 MTR_1g014860 47.761 67 28 1 54 120 21 80 2.44e-13 67.0
MS.gene072828.t1 MTR_1g014800 43.836 73 34 1 54 126 30 95 4.20e-13 66.6
MS.gene072828.t1 MTR_2g090235 48.485 66 27 1 54 119 217 275 4.59e-13 68.2
MS.gene072828.t1 MTR_5g008790 52.239 67 23 2 55 119 18 77 5.19e-13 65.1
MS.gene072828.t1 MTR_8g090350 36.036 111 57 3 54 159 112 213 6.18e-13 67.0
MS.gene072828.t1 MTR_2g435590 36.792 106 57 2 15 120 59 154 7.50e-13 66.2
MS.gene072828.t1 MTR_2g015050 39.216 102 56 1 16 117 95 190 8.49e-13 66.6
MS.gene072828.t1 MTR_2g043050 28.169 213 100 7 54 229 18 214 9.46e-13 65.5
MS.gene072828.t1 MTR_2g105380 36.522 115 60 3 7 121 75 176 1.72e-12 66.2
MS.gene072828.t1 MTR_5g062700 48.485 66 27 1 54 119 172 230 2.38e-12 65.9
MS.gene072828.t1 MTR_7g021010 45.333 75 33 2 46 119 2 69 2.53e-12 62.8
MS.gene072828.t1 MTR_1g087920 36.975 119 61 4 3 117 24 132 2.96e-12 65.1
MS.gene072828.t1 MTR_1g060910 40.964 83 22 1 39 121 3 58 3.25e-12 63.2
MS.gene072828.t1 MTR_2g043030 38.889 90 48 1 54 143 18 100 3.27e-12 63.9
MS.gene072828.t1 MTR_7g020980 46.377 69 30 1 51 119 47 108 3.33e-12 63.2
MS.gene072828.t1 MTR_3g105510 43.056 72 34 1 55 126 37 101 3.36e-12 64.3
MS.gene072828.t1 MTR_7g028925 46.269 67 26 2 54 120 28 84 3.56e-12 61.2
MS.gene072828.t1 MTR_7g021010 35.780 109 57 3 14 119 10 108 4.29e-12 62.8
MS.gene072828.t1 MTR_1g028560 38.235 102 52 2 17 118 81 171 8.04e-12 64.3
MS.gene072828.t1 MTR_4g088005 41.975 81 41 1 37 117 39 113 8.73e-12 60.8
MS.gene072828.t1 MTR_2g043040 38.889 90 48 1 54 143 18 100 9.96e-12 62.8
MS.gene072828.t1 MTR_8g006840 50.794 63 25 1 55 117 125 181 1.14e-11 62.4
MS.gene072828.t1 MTR_7g081805 49.206 63 26 1 55 117 61 117 2.05e-11 61.6
MS.gene072828.t1 MTR_7g081815 49.206 63 26 1 55 117 61 117 2.24e-11 61.6
MS.gene072828.t1 MTR_2g015040 32.381 105 57 2 13 117 29 119 2.68e-11 60.5
MS.gene072828.t1 MTR_5g083330 46.575 73 32 1 47 119 27 92 3.97e-11 62.0
MS.gene072828.t1 MTR_7g081795 49.206 63 26 1 55 117 60 116 4.17e-11 60.8
MS.gene072828.t1 MTR_3g105480 45.312 64 28 1 57 120 33 89 5.95e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072828.t1 AT1G12610 48.372 215 84 6 30 229 5 207 1.34e-56 179
MS.gene072828.t1 AT1G63030 47.059 204 77 5 30 229 5 181 6.63e-56 176
MS.gene072828.t1 AT1G63030 47.059 204 77 5 30 229 5 181 6.63e-56 176
MS.gene072828.t1 AT4G25470 46.610 236 90 10 4 229 7 216 9.10e-53 170
MS.gene072828.t1 AT5G51990 61.074 149 50 2 1 149 1 141 9.41e-53 170
MS.gene072828.t1 AT4G25480 46.383 235 97 8 5 229 1 216 1.81e-50 164
MS.gene072828.t1 AT4G25490 47.596 208 78 8 33 229 26 213 1.45e-45 151
MS.gene072828.t1 AT5G25810 56.842 95 34 1 51 145 32 119 4.89e-29 108
MS.gene072828.t1 AT5G11590 47.967 123 57 1 23 145 19 134 1.84e-28 107
MS.gene072828.t1 AT2G44940 56.989 93 33 1 51 143 96 181 6.69e-25 99.8
MS.gene072828.t1 AT2G35700 42.484 153 59 4 50 193 40 172 7.54e-24 94.7
MS.gene072828.t1 AT4G32800 51.579 95 39 1 51 145 15 102 1.37e-23 94.7
MS.gene072828.t1 AT4G16750 40.278 144 63 2 50 193 35 155 8.14e-22 89.0
MS.gene072828.t1 AT2G36450 60.000 80 24 2 51 129 11 83 1.61e-21 88.6
MS.gene072828.t1 AT1G01250 50.575 87 36 1 50 136 40 119 2.60e-21 88.2
MS.gene072828.t1 AT3G16280 52.326 86 34 1 51 136 57 135 4.42e-21 88.6
MS.gene072828.t1 AT3G16280 52.326 86 34 1 51 136 75 153 6.45e-21 88.6
MS.gene072828.t1 AT1G71450 33.908 174 91 3 54 227 23 172 8.75e-21 86.3
MS.gene072828.t1 AT3G60490 53.846 91 34 2 40 129 55 138 4.94e-20 86.3
MS.gene072828.t1 AT2G25820 61.765 68 19 1 54 121 25 85 1.29e-19 84.3
MS.gene072828.t1 AT5G52020 37.912 182 80 6 51 213 61 228 4.59e-16 74.7
MS.gene072828.t1 AT4G13620 45.882 85 38 2 36 119 212 289 4.72e-16 76.6
MS.gene072828.t1 AT1G74930 41.304 92 47 1 55 146 21 105 7.49e-16 73.6
MS.gene072828.t1 AT1G46768 54.286 70 24 2 50 119 27 88 7.58e-16 72.4
MS.gene072828.t1 AT1G46768 54.286 70 24 2 50 119 27 88 7.92e-16 72.4
MS.gene072828.t1 AT1G46768 54.286 70 24 2 50 119 27 88 7.92e-16 72.4
MS.gene072828.t1 AT1G19210 43.820 89 43 1 55 143 12 93 1.44e-15 72.4
MS.gene072828.t1 AT2G23340 43.564 101 43 3 55 148 28 121 3.21e-15 71.6
MS.gene072828.t1 AT1G21910 53.731 67 24 1 55 121 50 109 5.30e-15 72.0
MS.gene072828.t1 AT4G36900 50.000 72 29 1 46 117 21 85 5.70e-15 71.2
MS.gene072828.t1 AT3G50260 41.176 102 51 3 48 148 14 107 8.78e-15 69.7
MS.gene072828.t1 AT1G22190 39.823 113 46 3 7 119 50 140 8.89e-15 72.0
MS.gene072828.t1 AT4G39780 48.529 68 28 1 50 117 88 148 2.84e-14 70.5
MS.gene072828.t1 AT2G47520 37.963 108 56 2 10 117 9 105 3.96e-14 68.6
MS.gene072828.t1 AT1G77200 53.571 84 32 1 53 136 41 117 4.68e-14 69.7
MS.gene072828.t1 AT5G67190 50.794 63 24 1 55 117 21 76 4.76e-14 68.6
MS.gene072828.t1 AT2G22200 50.000 64 25 1 54 117 70 126 9.63e-14 68.9
MS.gene072828.t1 AT1G36060 51.562 64 24 1 54 117 142 198 1.46e-13 68.9
MS.gene072828.t1 AT4G31060 47.619 63 26 1 55 117 27 82 2.82e-13 66.2
MS.gene072828.t1 AT4G06746 46.377 69 30 1 55 123 36 97 2.97e-13 65.5
MS.gene072828.t1 AT5G65130 37.143 105 43 3 29 119 73 168 7.82e-13 66.6
MS.gene072828.t1 AT2G44840 53.968 63 23 1 55 117 92 148 8.16e-13 65.9
MS.gene072828.t1 AT5G65130 37.143 105 43 3 29 119 75 170 8.19e-13 66.6
MS.gene072828.t1 AT1G33760 46.739 92 42 1 55 146 20 104 9.04e-13 65.1
MS.gene072828.t1 AT1G78080 50.000 64 25 1 54 117 151 207 1.40e-12 66.2
MS.gene072828.t1 AT5G43410 44.156 77 35 2 41 117 3 71 1.48e-12 63.2
MS.gene072828.t1 AT2G20880 46.970 66 28 1 54 119 186 244 1.89e-12 65.9
MS.gene072828.t1 AT4G28140 46.970 66 28 1 54 119 142 200 2.08e-12 65.5
MS.gene072828.t1 AT3G16770 35.965 114 62 2 6 119 34 136 2.58e-12 64.7
MS.gene072828.t1 AT5G21960 46.269 67 29 1 53 119 5 64 3.42e-12 63.9
MS.gene072828.t1 AT5G07580 44.304 79 36 2 49 127 106 176 8.21e-12 62.8
MS.gene072828.t1 AT5G13330 41.237 97 40 4 37 133 28 107 8.89e-12 62.8
MS.gene072828.t1 AT1G12630 53.165 79 29 2 51 129 15 85 1.92e-11 61.6
MS.gene072828.t1 AT3G23240 41.250 80 41 1 55 134 82 155 1.99e-11 62.0
MS.gene072828.t1 AT3G23230 49.206 63 26 1 55 117 20 76 2.51e-11 60.1
MS.gene072828.t1 AT5G61890 49.254 67 25 2 51 117 88 145 4.97e-11 61.2
MS.gene072828.t1 AT5G05410 50.000 66 26 1 55 120 79 137 6.50e-11 61.2
MS.gene072828.t1 AT5G05410 50.794 63 24 1 55 117 79 134 6.65e-11 61.6
MS.gene072828.t1 AT3G11020 47.826 69 29 1 52 120 75 136 7.46e-11 61.2
MS.gene072828.t1 AT5G61600 52.239 67 24 3 51 117 86 144 8.10e-11 60.5

Find 50 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCAGGGAGAAGAGTGTTTA+AGG 0.162468 5.3:+4576303 MS.gene072828:CDS
CTTCTCCCTGCTCTCTTCTT+TGG 0.165077 5.3:-4576292 None:intergenic
CGTTGAAGATGGAGTATTTA+TGG 0.178815 5.3:+4576648 MS.gene072828:CDS
GTTGCTGCACTTGCTCTTAA+AGG 0.198231 5.3:+4576487 MS.gene072828:CDS
AAGTTCCTCCGAAGTGATTT+TGG 0.242855 5.3:+4576258 MS.gene072828:CDS
TGTGCACGTGCAGCCATTTC+AGG 0.278245 5.3:-4576460 None:intergenic
CTGTGCTTCTAGCACATTCT+TGG 0.281396 5.3:-4576683 None:intergenic
AAAACAATAAGTCAAGGATT+TGG 0.365596 5.3:+4576419 MS.gene072828:CDS
ACACTCAGATAGTTTCTTTG+TGG 0.402660 5.3:+4576839 MS.gene072828:CDS
TTACTACCATTGTAATTTGT+AGG 0.404075 5.3:-4576793 None:intergenic
TGCATGGCGGTTGACGTTGC+CGG 0.406208 5.3:+4576540 MS.gene072828:CDS
TGGTAGTAACTACAATGATA+TGG 0.406483 5.3:+4576807 MS.gene072828:CDS
TACTACCATTGTAATTTGTA+GGG 0.419201 5.3:-4576792 None:intergenic
TCGGAGGAACTTGTACGGTT+TGG 0.441704 5.3:-4576247 None:intergenic
AACATATCCAACTCCTGAAA+TGG 0.444364 5.3:+4576447 MS.gene072828:CDS
CGTGCAGCCATTTCAGGAGT+TGG 0.460218 5.3:-4576454 None:intergenic
TGTGCTAGAAGCACAGGTTC+CGG 0.470245 5.3:+4576690 MS.gene072828:CDS
CTAATGATACGGTGGAGATA+AGG 0.490158 5.3:+4576566 MS.gene072828:CDS
TGTTGTGTTACCGTTGAAGA+TGG 0.492239 5.3:+4576637 MS.gene072828:CDS
GGAGATAAGGAGAGCGGCGA+TGG 0.502163 5.3:+4576579 MS.gene072828:CDS
CAAGAATGTGCTAGAAGCAC+AGG 0.506339 5.3:+4576684 MS.gene072828:CDS
AATAAGTCAAGGATTTGGCT+TGG 0.507380 5.3:+4576424 MS.gene072828:CDS
GCACAACCAAAGAAGAGAGC+AGG 0.509106 5.3:+4576286 MS.gene072828:CDS
GGAGGCTGCTCAGTTGTTTG+CGG 0.520166 5.3:+4576600 MS.gene072828:CDS
CCATTGTAATTTGTAGGGGA+AGG 0.540315 5.3:-4576787 None:intergenic
TTGTAATTTGTAGGGGAAGG+AGG 0.542013 5.3:-4576784 None:intergenic
CATAAATACTCCATCTTCAA+CGG 0.545272 5.3:-4576647 None:intergenic
AGATGGAGTATTTATGGACA+TGG 0.547842 5.3:+4576654 MS.gene072828:CDS
TTGTTAACGTTATTGTTAGG+AGG 0.560970 5.3:-4576397 None:intergenic
CACAACCAAAGAAGAGAGCA+GGG 0.582496 5.3:+4576287 MS.gene072828:CDS
GAAGGAGGATTAGAACGTAA+AGG 0.587355 5.3:-4576769 None:intergenic
TGTATCGAGGAGTACGAAGA+AGG 0.588071 5.3:+4576341 MS.gene072828:CDS
CTTCGTACTCCTCGATACAC+TGG 0.590208 5.3:-4576337 None:intergenic
AGTAGTCGCCAAAAGATGAA+AGG 0.591499 5.3:-4576192 None:intergenic
GATAAGGAGAGCGGCGATGG+AGG 0.595614 5.3:+4576582 MS.gene072828:CDS
GAAGAAGGAACAATAACAAG+TGG 0.596284 5.3:+4576356 MS.gene072828:CDS
TCACTTCGGAGGAACTTGTA+CGG 0.606004 5.3:-4576252 None:intergenic
GAAGAGTGTTTAAGGAGACA+AGG 0.613517 5.3:+4576311 MS.gene072828:CDS
ACTACCATTGTAATTTGTAG+GGG 0.622305 5.3:-4576791 None:intergenic
TCAAATTCTGATGACACCAC+CGG 0.625258 5.3:-4576709 None:intergenic
GTTGCCGGAGTCTAATGATA+CGG 0.626022 5.3:+4576555 MS.gene072828:CDS
TGCAGATGCCAAAATCACTT+CGG 0.635482 5.3:-4576266 None:intergenic
AAGAAGGAACAATAACAAGT+GGG 0.657680 5.3:+4576357 MS.gene072828:CDS
CCTTCCCCTACAAATTACAA+TGG 0.665071 5.3:+4576787 MS.gene072828:CDS
GCTAGAAGCACAGGTTCCGG+TGG 0.665455 5.3:+4576693 MS.gene072828:CDS
ACAAGGCATCCAGTGTATCG+AGG 0.668565 5.3:+4576328 MS.gene072828:CDS
TACGGTGGAGATAAGGAGAG+CGG 0.678233 5.3:+4576573 MS.gene072828:CDS
AGATGCCAAAATCACTTCGG+AGG 0.680784 5.3:-4576263 None:intergenic
GCCGGAGTCTAATGATACGG+TGG 0.705686 5.3:+4576558 MS.gene072828:CDS
TCCACCGTATCATTAGACTC+CGG 0.706319 5.3:-4576559 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAACAAAAACAATAAGTCA+AGG + chr5.3:4576413-4576432 MS.gene072828:CDS 20.0%
!!! AATATTTTCCTTTCATCTTT+TGG + chr5.3:4576184-4576203 MS.gene072828:CDS 20.0%
!!! TTTTTGTTAACGTTATTGTT+AGG - chr5.3:4576403-4576422 None:intergenic 20.0%
! AAAACAATAAGTCAAGGATT+TGG + chr5.3:4576419-4576438 MS.gene072828:CDS 25.0%
!! TACTACCATTGTAATTTGTA+GGG - chr5.3:4576795-4576814 None:intergenic 25.0%
!! TTACTACCATTGTAATTTGT+AGG - chr5.3:4576796-4576815 None:intergenic 25.0%
AAGAAGGAACAATAACAAGT+GGG + chr5.3:4576357-4576376 MS.gene072828:CDS 30.0%
CATAAATACTCCATCTTCAA+CGG - chr5.3:4576650-4576669 None:intergenic 30.0%
TGGTAGTAACTACAATGATA+TGG + chr5.3:4576807-4576826 MS.gene072828:CDS 30.0%
TTGTTAACGTTATTGTTAGG+AGG - chr5.3:4576400-4576419 None:intergenic 30.0%
! ACTACCATTGTAATTTGTAG+GGG - chr5.3:4576794-4576813 None:intergenic 30.0%
AACATATCCAACTCCTGAAA+TGG + chr5.3:4576447-4576466 MS.gene072828:CDS 35.0%
AATAAGTCAAGGATTTGGCT+TGG + chr5.3:4576424-4576443 MS.gene072828:CDS 35.0%
ACACTCAGATAGTTTCTTTG+TGG + chr5.3:4576839-4576858 MS.gene072828:CDS 35.0%
GAAGAAGGAACAATAACAAG+TGG + chr5.3:4576356-4576375 MS.gene072828:CDS 35.0%
! AGATGGAGTATTTATGGACA+TGG + chr5.3:4576654-4576673 MS.gene072828:CDS 35.0%
! CGTTGAAGATGGAGTATTTA+TGG + chr5.3:4576648-4576667 MS.gene072828:CDS 35.0%
!!! TTAATTTTGCTGACTCTGCA+TGG + chr5.3:4576524-4576543 MS.gene072828:CDS 35.0%
AGTAGTCGCCAAAAGATGAA+AGG - chr5.3:4576195-4576214 None:intergenic 40.0%
CCTTCCCCTACAAATTACAA+TGG + chr5.3:4576787-4576806 MS.gene072828:CDS 40.0%
CTAATGATACGGTGGAGATA+AGG + chr5.3:4576566-4576585 MS.gene072828:CDS 40.0%
GAAGGAGGATTAGAACGTAA+AGG - chr5.3:4576772-4576791 None:intergenic 40.0%
TCAAATTCTGATGACACCAC+CGG - chr5.3:4576712-4576731 None:intergenic 40.0%
TGCAGATGCCAAAATCACTT+CGG - chr5.3:4576269-4576288 None:intergenic 40.0%
TGTTGTGTTACCGTTGAAGA+TGG + chr5.3:4576637-4576656 MS.gene072828:CDS 40.0%
! AAGTTCCTCCGAAGTGATTT+TGG + chr5.3:4576258-4576277 MS.gene072828:CDS 40.0%
! CCATTGTAATTTGTAGGGGA+AGG - chr5.3:4576790-4576809 None:intergenic 40.0%
! TTGTAATTTGTAGGGGAAGG+AGG - chr5.3:4576787-4576806 None:intergenic 40.0%
!! GAAGAGTGTTTAAGGAGACA+AGG + chr5.3:4576311-4576330 MS.gene072828:CDS 40.0%
AGATGCCAAAATCACTTCGG+AGG - chr5.3:4576266-4576285 None:intergenic 45.0%
CACAACCAAAGAAGAGAGCA+GGG + chr5.3:4576287-4576306 MS.gene072828:CDS 45.0%
CTGTGCTTCTAGCACATTCT+TGG - chr5.3:4576686-4576705 None:intergenic 45.0%
GTTGCCGGAGTCTAATGATA+CGG + chr5.3:4576555-4576574 MS.gene072828:CDS 45.0%
GTTGCTGCACTTGCTCTTAA+AGG + chr5.3:4576487-4576506 MS.gene072828:CDS 45.0%
TCACTTCGGAGGAACTTGTA+CGG - chr5.3:4576255-4576274 None:intergenic 45.0%
TCCACCGTATCATTAGACTC+CGG - chr5.3:4576562-4576581 None:intergenic 45.0%
! CAAGAATGTGCTAGAAGCAC+AGG + chr5.3:4576684-4576703 MS.gene072828:CDS 45.0%
! TGTATCGAGGAGTACGAAGA+AGG + chr5.3:4576341-4576360 MS.gene072828:CDS 45.0%
!! AGCAGGGAGAAGAGTGTTTA+AGG + chr5.3:4576303-4576322 MS.gene072828:CDS 45.0%
!!! ATTTTGCTGACTCTGCATGG+CGG + chr5.3:4576527-4576546 MS.gene072828:CDS 45.0%
CTTCGTACTCCTCGATACAC+TGG - chr5.3:4576340-4576359 None:intergenic 50.0%
CTTCTCCCTGCTCTCTTCTT+TGG - chr5.3:4576295-4576314 None:intergenic 50.0%
GCACAACCAAAGAAGAGAGC+AGG + chr5.3:4576286-4576305 MS.gene072828:CDS 50.0%
TACGGTGGAGATAAGGAGAG+CGG + chr5.3:4576573-4576592 MS.gene072828:CDS 50.0%
TCGGAGGAACTTGTACGGTT+TGG - chr5.3:4576250-4576269 None:intergenic 50.0%
! ACAAGGCATCCAGTGTATCG+AGG + chr5.3:4576328-4576347 MS.gene072828:CDS 50.0%
! TGTGCTAGAAGCACAGGTTC+CGG + chr5.3:4576690-4576709 MS.gene072828:CDS 50.0%
CGTGCAGCCATTTCAGGAGT+TGG - chr5.3:4576457-4576476 None:intergenic 55.0%
GCCGGAGTCTAATGATACGG+TGG + chr5.3:4576558-4576577 MS.gene072828:CDS 55.0%
GGAGGCTGCTCAGTTGTTTG+CGG + chr5.3:4576600-4576619 MS.gene072828:CDS 55.0%
TGTGCACGTGCAGCCATTTC+AGG - chr5.3:4576463-4576482 None:intergenic 55.0%
GATAAGGAGAGCGGCGATGG+AGG + chr5.3:4576582-4576601 MS.gene072828:CDS 60.0%
GGAGATAAGGAGAGCGGCGA+TGG + chr5.3:4576579-4576598 MS.gene072828:CDS 60.0%
TGCATGGCGGTTGACGTTGC+CGG + chr5.3:4576540-4576559 MS.gene072828:CDS 60.0%
! GCTAGAAGCACAGGTTCCGG+TGG + chr5.3:4576693-4576712 MS.gene072828:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 4576181 4576876 4576181 ID=MS.gene072828
chr5.3 mRNA 4576181 4576876 4576181 ID=MS.gene072828.t1;Parent=MS.gene072828
chr5.3 exon 4576181 4576876 4576181 ID=MS.gene072828.t1.exon1;Parent=MS.gene072828.t1
chr5.3 CDS 4576181 4576876 4576181 ID=cds.MS.gene072828.t1;Parent=MS.gene072828.t1
Gene Sequence

>MS.gene072828

ATGAATATTTTCCTTTCATCTTTTGGCGACTACTCAGATTCTTCTTCTTCTTCAGAAACAAGTTCACCAAACCGTACAAGTTCCTCCGAAGTGATTTTGGCATCTGCACAACCAAAGAAGAGAGCAGGGAGAAGAGTGTTTAAGGAGACAAGGCATCCAGTGTATCGAGGAGTACGAAGAAGGAACAATAACAAGTGGGTTTGTGAGATGAGAGTTCCTCCTAACAATAACGTTAACAAAAACAATAAGTCAAGGATTTGGCTTGGAACATATCCAACTCCTGAAATGGCTGCACGTGCACATGATGTTGCTGCACTTGCTCTTAAAGGAAAATCAGCTTGTCTTAATTTTGCTGACTCTGCATGGCGGTTGACGTTGCCGGAGTCTAATGATACGGTGGAGATAAGGAGAGCGGCGATGGAGGCTGCTCAGTTGTTTGCGGTTGAAGACAAGCAGTGTTGTGTTACCGTTGAAGATGGAGTATTTATGGACATGGAAGATTCCAAGAATGTGCTAGAAGCACAGGTTCCGGTGGTGTCATCAGAATTTGAAGACATGCATGATTTGATTTTGAGTATTGCTAATGAGCCTTTACGTTCTAATCCTCCTTCCCCTACAAATTACAATGGTAGTAACTACAATGATATGGAGATATTTGACACTCAGATAGTTTCTTTGTGGAACTTCTCAATTTGA
Protein sequence

>MS.gene072828.t1

MNIFLSSFGDYSDSSSSSETSSPNRTSSSEVILASAQPKKRAGRRVFKETRHPVYRGVRRRNNNKWVCEMRVPPNNNVNKNNKSRIWLGTYPTPEMAARAHDVAALALKGKSACLNFADSAWRLTLPESNDTVEIRRAAMEAAQLFAVEDKQCCVTVEDGVFMDMEDSKNVLEAQVPVVSSEFEDMHDLILSIANEPLRSNPPSPTNYNGSNYNDMEIFDTQIVSLWNFSI