Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052190 | MS.gene072902 | 0.80839 | 3.17E-50 | -1.69E-46 |
| MS.gene054083 | MS.gene072902 | 0.804853 | 1.77E-49 | -1.69E-46 |
| MS.gene055356 | MS.gene072902 | 0.823097 | 1.66E-53 | -1.69E-46 |
| MS.gene055701 | MS.gene072902 | 0.817006 | 4.13E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 121 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTCTTAATTATGGACTGGC+TGG | 0.371969 | 3.2:+87129788 | MS.gene072902:CDS |
| CGCGTGTGCAGTGCTGTTAA+TGG | 0.405979 | 3.2:+87129607 | MS.gene072902:CDS |
| TGGTTCCGCTGTCGTATCAT+TGG | 0.413172 | 3.2:+87129627 | MS.gene072902:CDS |
| ACAGACATGACAGTGAGGTT+AGG | 0.413385 | 3.2:+87128432 | None:intergenic |
| CCATTTGTATTGTCATGTAA+TGG | 0.427101 | 3.2:-87129236 | None:intergenic |
| GATGAAACGAGCATTCAATA+TGG | 0.429660 | 3.2:+87129173 | MS.gene072902:CDS |
| CAGACATGACAGTGAGGTTA+GGG | 0.436195 | 3.2:+87128433 | None:intergenic |
| TTGTCCTCTTAATTATGGAC+TGG | 0.440524 | 3.2:+87129784 | MS.gene072902:CDS |
| CCATTACATGACAATACAAA+TGG | 0.482786 | 3.2:+87129236 | MS.gene072902:CDS |
| CCAGCCAGTCCATAATTAAG+AGG | 0.489147 | 3.2:-87129788 | None:intergenic |
| CGCGTTCGTTGCCACTCGAA+CGG | 0.515131 | 3.2:-87129588 | None:intergenic |
| TCCGCTGTCGTATCATTGGC+GGG | 0.519856 | 3.2:+87129631 | MS.gene072902:CDS |
| TCTATCTGTGTTGTGCATAC+AGG | 0.524362 | 3.2:-87129210 | None:intergenic |
| TTCCGCTGTCGTATCATTGG+CGG | 0.541165 | 3.2:+87129630 | MS.gene072902:CDS |
| CTCTTAATTATGGACTGGCT+GGG | 0.565967 | 3.2:+87129789 | MS.gene072902:CDS |
| CGAATGCTTGATCTCTTCAA+CGG | 0.578302 | 3.2:-87129146 | None:intergenic |
| TATGTCGCAGACATTGGTCG+TGG | 0.580047 | 3.2:+87129732 | MS.gene072902:intron |
| ACGAGCATTCAATATGGTGG+AGG | 0.602271 | 3.2:+87129179 | MS.gene072902:CDS |
| AAGCTTATCAACCGTTCGAG+TGG | 0.627405 | 3.2:+87129577 | MS.gene072902:CDS |
| GAAGAGATCAAGCATTCGAA+CGG | 0.639249 | 3.2:+87129151 | MS.gene072902:CDS |
| TCTGTGTTGTGCATACAGGA+AGG | 0.640642 | 3.2:-87129206 | None:intergenic |
| GGGCTTACACTTATTAAACA+CGG | 0.640828 | 3.2:+87128453 | MS.gene072902:CDS |
| GAAACGAGCATTCAATATGG+TGG | 0.640888 | 3.2:+87129176 | MS.gene072902:CDS |
| CATTGGTCGTGGCAAAACAG+AGG | 0.682445 | 3.2:+87129743 | MS.gene072902:CDS |
| ATCTAACAGACATGACAGTG+AGG | 0.727664 | 3.2:+87128427 | None:intergenic |
| ACCCGCCAATGATACGACAG+CGG | 0.741993 | 3.2:-87129632 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATATTACTTTAATTGTATT+TGG | + | chr3.2:87130198-87130217 | MS.gene072902:intron | 10.0% |
| !! | AAACAAATTATAAACTCAAA+AGG | - | chr3.2:87130037-87130056 | None:intergenic | 15.0% |
| !! | TAAAGATCAAATAAATTATG+AGG | - | chr3.2:87129369-87129388 | None:intergenic | 15.0% |
| !!! | AATAAAGGTGTAAATTTATA+AGG | - | chr3.2:87129712-87129731 | None:intergenic | 15.0% |
| !! | GACAAAAAAATCTTCATATA+AGG | - | chr3.2:87129769-87129788 | None:intergenic | 20.0% |
| !!! | TTTTTTTGTCCTCTTAATTA+TGG | + | chr3.2:87129779-87129798 | MS.gene072902:CDS | 20.0% |
| ! | AACCAAAAAATTAAAGCAGA+AGG | - | chr3.2:87128760-87128779 | None:intergenic | 25.0% |
| ! | AAGCAGAATAAGATTGTATT+TGG | + | chr3.2:87130083-87130102 | MS.gene072902:intron | 25.0% |
| ! | AATCCACTGAAATAATGAAA+AGG | - | chr3.2:87128789-87128808 | None:intergenic | 25.0% |
| ! | ACAATTAGCTACAAATGTTT+CGG | + | chr3.2:87128867-87128886 | MS.gene072902:intron | 25.0% |
| ! | ACATGGTAATCAATATTTAC+AGG | + | chr3.2:87128727-87128746 | MS.gene072902:intron | 25.0% |
| ! | AGAGTGAGAAAACATAATTT+GGG | - | chr3.2:87129906-87129925 | None:intergenic | 25.0% |
| ! | ATCCACTGAAATAATGAAAA+GGG | - | chr3.2:87128788-87128807 | None:intergenic | 25.0% |
| ! | ATCTTGAAATTGTATTTACC+AGG | - | chr3.2:87129554-87129573 | None:intergenic | 25.0% |
| ! | ATTATAATTAGCGAGTCAAT+TGG | - | chr3.2:87129952-87129971 | None:intergenic | 25.0% |
| ! | CTAATTATAATACAACCCTT+CGG | + | chr3.2:87129961-87129980 | MS.gene072902:intron | 25.0% |
| ! | GAATAAGATTGTATTTGGTA+TGG | + | chr3.2:87130088-87130107 | MS.gene072902:intron | 25.0% |
| ! | TGGTAAATACAATTTCAAGA+TGG | + | chr3.2:87129553-87129572 | MS.gene072902:intron | 25.0% |
| ! | TTATAATTAGCGAGTCAATT+GGG | - | chr3.2:87129951-87129970 | None:intergenic | 25.0% |
| !! | TTCTGCTTTAAATCAATTTG+AGG | - | chr3.2:87130071-87130090 | None:intergenic | 25.0% |
| !!! | TTCCTTCTGCTTTAATTTTT+TGG | + | chr3.2:87128755-87128774 | MS.gene072902:intron | 25.0% |
| !!! | TTGTCCTTTTAATTATTGAC+TGG | + | chr3.2:87129839-87129858 | MS.gene072902:intron | 25.0% |
| AATGTCTGCGACATAAATAA+AGG | - | chr3.2:87129727-87129746 | None:intergenic | 30.0% | |
| AGTGAGAAAACATAATTTGG+GGG | - | chr3.2:87129904-87129923 | None:intergenic | 30.0% | |
| ATTGTACAGTGGTAGTTATA+AGG | + | chr3.2:87130120-87130139 | MS.gene072902:intron | 30.0% | |
| CAGAGTGAGAAAACATAATT+TGG | - | chr3.2:87129907-87129926 | None:intergenic | 30.0% | |
| CCAACCAGTCAATAATTAAA+AGG | - | chr3.2:87129846-87129865 | None:intergenic | 30.0% | |
| CCATTACATGACAATACAAA+TGG | + | chr3.2:87129236-87129255 | MS.gene072902:CDS | 30.0% | |
| CCATTTGTATTGTCATGTAA+TGG | - | chr3.2:87129239-87129258 | None:intergenic | 30.0% | |
| GAGTGAGAAAACATAATTTG+GGG | - | chr3.2:87129905-87129924 | None:intergenic | 30.0% | |
| GTCAAGATCATGTGAAAATA+AGG | - | chr3.2:87129443-87129462 | None:intergenic | 30.0% | |
| TCAATAATCATACACAACTC+AGG | - | chr3.2:87128573-87128592 | None:intergenic | 30.0% | |
| TCAGTAGCAAGACAAAATAA+CGG | - | chr3.2:87129290-87129309 | None:intergenic | 30.0% | |
| TGTAGGGTTTATTGTAAACA+AGG | + | chr3.2:87129006-87129025 | MS.gene072902:intron | 30.0% | |
| TTGTACAGTGGTAGTTATAA+GGG | + | chr3.2:87130121-87130140 | MS.gene072902:intron | 30.0% | |
| TTGTTCCCAAACTGAAATAA+TGG | + | chr3.2:87129066-87129085 | MS.gene072902:intron | 30.0% | |
| TTTATTTATGTCGCAGACAT+TGG | + | chr3.2:87129726-87129745 | MS.gene072902:intron | 30.0% | |
| ! | ATCAATTTGAGGCACAAAAA+AGG | - | chr3.2:87130060-87130079 | None:intergenic | 30.0% |
| ! | TGCTATTTATTCCATAGCAT+CGG | + | chr3.2:87129115-87129134 | MS.gene072902:intron | 30.0% |
| ! | TTCCCTTTTCATTATTTCAG+TGG | + | chr3.2:87128783-87128802 | MS.gene072902:intron | 30.0% |
| !! | CCTTTTAATTATTGACTGGT+TGG | + | chr3.2:87129843-87129862 | MS.gene072902:intron | 30.0% |
| AACAAACTGTACAGTTTCGT+TGG | - | chr3.2:87129469-87129488 | None:intergenic | 35.0% | |
| AATCAAATCAGTGGCTTTCA+TGG | + | chr3.2:87129518-87129537 | MS.gene072902:intron | 35.0% | |
| AGAAAACATAATTTGGGGGT+GGG | - | chr3.2:87129900-87129919 | None:intergenic | 35.0% | |
| CATCGCCATTATTTCAGTTT+GGG | - | chr3.2:87129074-87129093 | None:intergenic | 35.0% | |
| GAATTTATTGAACCGCGAAT+TGG | + | chr3.2:87128939-87128958 | MS.gene072902:intron | 35.0% | |
| GATGAAACGAGCATTCAATA+TGG | + | chr3.2:87129173-87129192 | MS.gene072902:CDS | 35.0% | |
| GGGCTTACACTTATTAAACA+CGG | + | chr3.2:87128453-87128472 | MS.gene072902:CDS | 35.0% | |
| GGTAGAATAAGATTGTACAG+TGG | + | chr3.2:87130109-87130128 | MS.gene072902:intron | 35.0% | |
| TACACAACTCAGGAAAGAAA+AGG | - | chr3.2:87128563-87128582 | None:intergenic | 35.0% | |
| TCTAATGACTTCTTGCAAAG+AGG | - | chr3.2:87130011-87130030 | None:intergenic | 35.0% | |
| TTGATTACCATGTGCTAATG+AGG | - | chr3.2:87128720-87128739 | None:intergenic | 35.0% | |
| TTGTCCTCTTAATTATGGAC+TGG | + | chr3.2:87129784-87129803 | MS.gene072902:CDS | 35.0% | |
| ! | ACGAAACTGTACAGTTTGTT+CGG | + | chr3.2:87129469-87129488 | MS.gene072902:intron | 35.0% |
| ! | TCAATTTAGTTTCAACGCAC+TGG | + | chr3.2:87128611-87128630 | MS.gene072902:intron | 35.0% |
| ! | TGGGAAGTACAACAAATAGA+TGG | - | chr3.2:87129932-87129951 | None:intergenic | 35.0% |
| !! | TTCAGTGGATTGATTCATTC+TGG | + | chr3.2:87128798-87128817 | MS.gene072902:intron | 35.0% |
| AACTGTACAGTTTCGTTGGA+CGG | - | chr3.2:87129465-87129484 | None:intergenic | 40.0% | |
| ACTAAAAGGAAGACAGGTGT+AGG | + | chr3.2:87128989-87129008 | MS.gene072902:intron | 40.0% | |
| AGCGAAGATGAAGATGAAGA+AGG | + | chr3.2:87128899-87128918 | MS.gene072902:intron | 40.0% | |
| CCAAACTGAAATAATGGCGA+TGG | + | chr3.2:87129072-87129091 | MS.gene072902:intron | 40.0% | |
| CCATCGCCATTATTTCAGTT+TGG | - | chr3.2:87129075-87129094 | None:intergenic | 40.0% | |
| CGAATGCTTGATCTCTTCAA+CGG | - | chr3.2:87129149-87129168 | None:intergenic | 40.0% | |
| CGTCATAGCAATCAAATCAG+TGG | + | chr3.2:87129509-87129528 | MS.gene072902:intron | 40.0% | |
| CTAAAAGGAAGACAGGTGTA+GGG | + | chr3.2:87128990-87129009 | MS.gene072902:intron | 40.0% | |
| GAAACGAGCATTCAATATGG+TGG | + | chr3.2:87129176-87129195 | MS.gene072902:CDS | 40.0% | |
| GAAGAGATCAAGCATTCGAA+CGG | + | chr3.2:87129151-87129170 | MS.gene072902:CDS | 40.0% | |
| GAGAAAACATAATTTGGGGG+TGG | - | chr3.2:87129901-87129920 | None:intergenic | 40.0% | |
| GGTTTGAATGCAAAGACAGA+AGG | - | chr3.2:87129269-87129288 | None:intergenic | 40.0% | |
| TCTATCTGTGTTGTGCATAC+AGG | - | chr3.2:87129213-87129232 | None:intergenic | 40.0% | |
| TTATTGAACCGCGAATTGGA+TGG | + | chr3.2:87128943-87128962 | MS.gene072902:intron | 40.0% | |
| ! | AACTGTACAGTTTGTTCGGT+TGG | + | chr3.2:87129473-87129492 | MS.gene072902:intron | 40.0% |
| !! | CTCTTAATTATGGACTGGCT+GGG | + | chr3.2:87129789-87129808 | MS.gene072902:CDS | 40.0% |
| !! | GTGTAATGTCTTGCTGTTTG+TGG | + | chr3.2:87129662-87129681 | MS.gene072902:intron | 40.0% |
| AAACACGGTGAGTCTAGACA+CGG | + | chr3.2:87128468-87128487 | MS.gene072902:intron | 45.0% | |
| AAGCTTATCAACCGTTCGAG+TGG | + | chr3.2:87129577-87129596 | MS.gene072902:CDS | 45.0% | |
| ACGAGCATTCAATATGGTGG+AGG | + | chr3.2:87129179-87129198 | MS.gene072902:CDS | 45.0% | |
| ACTGCATCATCATATGCGTG+TGG | - | chr3.2:87129328-87129347 | None:intergenic | 45.0% | |
| CAGGTGACTAAAAGGAAGAC+AGG | + | chr3.2:87128983-87129002 | MS.gene072902:intron | 45.0% | |
| CCAGCCAGTCCATAATTAAG+AGG | - | chr3.2:87129791-87129810 | None:intergenic | 45.0% | |
| TCTGTGTTGTGCATACAGGA+AGG | - | chr3.2:87129209-87129228 | None:intergenic | 45.0% | |
| TCTTCGATCCATCCAATTCG+CGG | - | chr3.2:87128954-87128973 | None:intergenic | 45.0% | |
| TGTAGCAGCAGGTGACTAAA+AGG | + | chr3.2:87128975-87128994 | MS.gene072902:intron | 45.0% | |
| ! | GTTTGAGATGGTGCACTAGA+CGG | + | chr3.2:87129983-87130002 | MS.gene072902:intron | 45.0% |
| ! | TACAACCCTTCGGTTTGAGA+TGG | + | chr3.2:87129971-87129990 | MS.gene072902:intron | 45.0% |
| !! | CCTCTTAATTATGGACTGGC+TGG | + | chr3.2:87129788-87129807 | MS.gene072902:CDS | 45.0% |
| AATCGGGTGGAGTTAACGGA+GGG | + | chr3.2:87128675-87128694 | MS.gene072902:intron | 50.0% | |
| ACAACAGTGAGAGGGGATTG+CGG | + | chr3.2:87128638-87128657 | MS.gene072902:intron | 50.0% | |
| ACTGGCAACACAACAGTGAG+AGG | + | chr3.2:87128629-87128648 | MS.gene072902:intron | 50.0% | |
| AGGGAATCGGGTGGAGTTAA+CGG | + | chr3.2:87128671-87128690 | MS.gene072902:intron | 50.0% | |
| AGTGCACCATCTCAAACCGA+AGG | - | chr3.2:87129980-87129999 | None:intergenic | 50.0% | |
| CATTGGTCGTGGCAAAACAG+AGG | + | chr3.2:87129743-87129762 | MS.gene072902:CDS | 50.0% | |
| CTGGCAACACAACAGTGAGA+GGG | + | chr3.2:87128630-87128649 | MS.gene072902:intron | 50.0% | |
| GATCGCAGTTCTGCTAAACG+CGG | + | chr3.2:87128506-87128525 | MS.gene072902:intron | 50.0% | |
| GTCTCAGCCTCATTAGCACA+TGG | + | chr3.2:87128710-87128729 | MS.gene072902:intron | 50.0% | |
| GTGCACCATCTCAAACCGAA+GGG | - | chr3.2:87129979-87129998 | None:intergenic | 50.0% | |
| TATGTCGCAGACATTGGTCG+TGG | + | chr3.2:87129732-87129751 | MS.gene072902:intron | 50.0% | |
| TGGCAACACAACAGTGAGAG+GGG | + | chr3.2:87128631-87128650 | MS.gene072902:intron | 50.0% | |
| TGGTTCCGCTGTCGTATCAT+TGG | + | chr3.2:87129627-87129646 | MS.gene072902:CDS | 50.0% | |
| TTCCGCTGTCGTATCATTGG+CGG | + | chr3.2:87129630-87129649 | MS.gene072902:CDS | 50.0% | |
| TTTCATGGTCGACTGCTTCC+TGG | + | chr3.2:87129533-87129552 | MS.gene072902:intron | 50.0% | |
| ACCCGCCAATGATACGACAG+CGG | - | chr3.2:87129635-87129654 | None:intergenic | 55.0% | |
| GAATCGGGTGGAGTTAACGG+AGG | + | chr3.2:87128674-87128693 | MS.gene072902:intron | 55.0% | |
| GGATCGAAGACTGTAGCAGC+AGG | + | chr3.2:87128964-87128983 | MS.gene072902:intron | 55.0% | |
| TCCGCTGTCGTATCATTGGC+GGG | + | chr3.2:87129631-87129650 | MS.gene072902:CDS | 55.0% | |
| TGCTAAACGCGGCGGCAATT+TGG | + | chr3.2:87128517-87128536 | MS.gene072902:intron | 55.0% | |
| ! | CGCGTGTGCAGTGCTGTTAA+TGG | + | chr3.2:87129607-87129626 | MS.gene072902:CDS | 55.0% |
| AACGCGGCGGCAATTTGGCA+AGG | + | chr3.2:87128522-87128541 | MS.gene072902:intron | 60.0% | |
| ACAGTGAGAGGGGATTGCGG+TGG | + | chr3.2:87128641-87128660 | MS.gene072902:intron | 60.0% | |
| CACGGTGAGTCTAGACACGG+CGG | + | chr3.2:87128471-87128490 | MS.gene072902:intron | 60.0% | |
| CGCAGTTCTGCTAAACGCGG+CGG | + | chr3.2:87128509-87128528 | MS.gene072902:intron | 60.0% | |
| CGCGTTCGTTGCCACTCGAA+CGG | - | chr3.2:87129591-87129610 | None:intergenic | 60.0% | |
| CGGTCGCTTGTCCGATGCTA+TGG | - | chr3.2:87129129-87129148 | None:intergenic | 60.0% | |
| GGGTGGAGTTAACGGAGGGA+GGG | + | chr3.2:87128679-87128698 | MS.gene072902:intron | 60.0% | |
| ! | CGGTGGTGGTGAGAGGGAAT+CGG | + | chr3.2:87128658-87128677 | MS.gene072902:intron | 60.0% |
| ! | GGATTGCGGTGGTGGTGAGA+GGG | + | chr3.2:87128652-87128671 | MS.gene072902:intron | 60.0% |
| ! | GGTGGTGGTGAGAGGGAATC+GGG | + | chr3.2:87128659-87128678 | MS.gene072902:intron | 60.0% |
| CGGGTGGAGTTAACGGAGGG+AGG | + | chr3.2:87128678-87128697 | MS.gene072902:intron | 65.0% | |
| GGTGGTGAGAGGGAATCGGG+TGG | + | chr3.2:87128662-87128681 | MS.gene072902:intron | 65.0% | |
| GTGAGAGGGGATTGCGGTGG+TGG | + | chr3.2:87128644-87128663 | MS.gene072902:intron | 65.0% | |
| ! | GGGATTGCGGTGGTGGTGAG+AGG | + | chr3.2:87128651-87128670 | MS.gene072902:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 87128438 | 87130233 | 87128438 | ID=MS.gene072902 |
| chr3.2 | mRNA | 87128438 | 87130233 | 87128438 | ID=MS.gene072902.t1;Parent=MS.gene072902 |
| chr3.2 | exon | 87128438 | 87128474 | 87128438 | ID=MS.gene072902.t1.exon1;Parent=MS.gene072902.t1 |
| chr3.2 | CDS | 87128438 | 87128474 | 87128438 | ID=cds.MS.gene072902.t1;Parent=MS.gene072902.t1 |
| chr3.2 | exon | 87129132 | 87129257 | 87129132 | ID=MS.gene072902.t1.exon2;Parent=MS.gene072902.t1 |
| chr3.2 | CDS | 87129132 | 87129257 | 87129132 | ID=cds.MS.gene072902.t1;Parent=MS.gene072902.t1 |
| chr3.2 | exon | 87129572 | 87129652 | 87129572 | ID=MS.gene072902.t1.exon3;Parent=MS.gene072902.t1 |
| chr3.2 | CDS | 87129572 | 87129652 | 87129572 | ID=cds.MS.gene072902.t1;Parent=MS.gene072902.t1 |
| chr3.2 | exon | 87129742 | 87129810 | 87129742 | ID=MS.gene072902.t1.exon4;Parent=MS.gene072902.t1 |
| chr3.2 | CDS | 87129742 | 87129810 | 87129742 | ID=cds.MS.gene072902.t1;Parent=MS.gene072902.t1 |
| chr3.2 | exon | 87130229 | 87130233 | 87130229 | ID=MS.gene072902.t1.exon5;Parent=MS.gene072902.t1 |
| chr3.2 | CDS | 87130229 | 87130233 | 87130229 | ID=cds.MS.gene072902.t1;Parent=MS.gene072902.t1 |
| Gene Sequence |
| Protein sequence |
