Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072981.t1 | AES73754.2 | 97.2 | 542 | 15 | 0 | 1 | 542 | 250 | 791 | 1.20E-290 | 1008.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072981.t1 | G7JCA1 | 97.2 | 542 | 15 | 0 | 1 | 542 | 250 | 791 | 8.8e-291 | 1008.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060347 | MS.gene072981 | 0.809 | 2.35E-50 | -1.69E-46 |
| MS.gene060349 | MS.gene072981 | 0.849864 | 2.46E-60 | -1.69E-46 |
| MS.gene060350 | MS.gene072981 | 0.821847 | 3.25E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 104 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGAAGAATCTTCTCTTTA+TGG | 0.065700 | 3.3:+88351526 | MS.gene072981:CDS |
| CACTGTAAATCTTTAGAATT+TGG | 0.112071 | 3.3:-88348228 | None:intergenic |
| TATCATTATGCTCGTGTTTC+TGG | 0.148332 | 3.3:+88351148 | MS.gene072981:CDS |
| TTTGTTGCTTGTGTGAATTC+TGG | 0.205210 | 3.3:+88351725 | MS.gene072981:CDS |
| GAATCAAGTGTTCTTGCTTT+AGG | 0.207970 | 3.3:+88353969 | MS.gene072981:CDS |
| TGCCATGGGTTGGGATTTAT+TGG | 0.208837 | 3.3:+88351435 | MS.gene072981:CDS |
| ATATCAAGAAGTGAGGATTT+TGG | 0.246515 | 3.3:+88348181 | MS.gene072981:CDS |
| TTGCATAGTGATCAATTCTA+TGG | 0.274245 | 3.3:-88352857 | None:intergenic |
| ATCCTCACTTCTTGATATAA+TGG | 0.297071 | 3.3:-88348176 | None:intergenic |
| TTTCTGTCCAAACTCCTATT+CGG | 0.336377 | 3.3:+88351861 | MS.gene072981:CDS |
| TTGTCCCTGGCATAAAATTC+CGG | 0.338778 | 3.3:+88352830 | MS.gene072981:CDS |
| AAATAAAGTATTACCCGAAT+AGG | 0.345684 | 3.3:-88351875 | None:intergenic |
| TTTAGTAGCTGCCATGGGTT+GGG | 0.361566 | 3.3:+88351426 | MS.gene072981:CDS |
| TTAACCAATGCATCTTCTTC+GGG | 0.373794 | 3.3:+88355766 | MS.gene072981:CDS |
| CTCAACAAAATGATCAGAAT+TGG | 0.375867 | 3.3:-88351819 | None:intergenic |
| GTATTACCCGAATAGGAGTT+TGG | 0.377854 | 3.3:-88351868 | None:intergenic |
| GAAGGATTTGCGGAGCCATT+TGG | 0.380313 | 3.3:+88354055 | MS.gene072981:CDS |
| GAAAATTAATGCAACTTCTC+TGG | 0.381683 | 3.3:+88351479 | MS.gene072981:CDS |
| ATCATAAGCATACCTCTTCC+AGG | 0.383930 | 3.3:-88352902 | None:intergenic |
| CTCAAGGGTTGCTTTGTCTT+CGG | 0.388566 | 3.3:-88356399 | None:intergenic |
| CATAATTTCACTGCTGCATA+TGG | 0.388796 | 3.3:+88355690 | MS.gene072981:CDS |
| GTTAACCAATGCATCTTCTT+CGG | 0.391953 | 3.3:+88355765 | MS.gene072981:CDS |
| ATACACTGCTAGCTTCTTCC+AGG | 0.399058 | 3.3:-88351228 | None:intergenic |
| TGATCAATTCTATGGCTTCC+CGG | 0.400945 | 3.3:-88352849 | None:intergenic |
| CCACTCGAGGGCCTGTCTGC+TGG | 0.402590 | 3.3:-88355929 | None:intergenic |
| TGTACGTCTTCAAAACTATC+TGG | 0.405268 | 3.3:+88351045 | MS.gene072981:CDS |
| TTCTGTCCAAACTCCTATTC+GGG | 0.405375 | 3.3:+88351862 | MS.gene072981:CDS |
| CAAGTAAGTCACAAGTAATT+CGG | 0.418420 | 3.3:+88351503 | MS.gene072981:CDS |
| CAGAAAGCAAAGTTTGATAT+AGG | 0.423959 | 3.3:+88356344 | MS.gene072981:CDS |
| TCCAAGTTCGTACCAGGCTT+CGG | 0.424401 | 3.3:-88351073 | None:intergenic |
| TCTAATTCAACCACAGATGA+TGG | 0.427920 | 3.3:-88355877 | None:intergenic |
| CTTTAGTAGCTGCCATGGGT+TGG | 0.428809 | 3.3:+88351425 | MS.gene072981:CDS |
| CAATGCGTTTCATTGAAGAA+TGG | 0.429876 | 3.3:+88355965 | MS.gene072981:CDS |
| AATGCGTTTCATTGAAGAAT+GGG | 0.434098 | 3.3:+88355966 | MS.gene072981:CDS |
| AACCCTTGAGTTAGCTGAAT+GGG | 0.436412 | 3.3:+88356412 | MS.gene072981:CDS |
| CACTCACAGGTTGAGTAGCT+TGG | 0.439566 | 3.3:-88356082 | None:intergenic |
| TGTCCCTGGCATAAAATTCC+GGG | 0.447173 | 3.3:+88352831 | MS.gene072981:CDS |
| TGCTGTGAAAAGGGAGCACC+TGG | 0.456845 | 3.3:+88351210 | MS.gene072981:CDS |
| TTGCTTCCACTCTGGAAGCC+TGG | 0.460495 | 3.3:+88352884 | MS.gene072981:CDS |
| CTGCCCTGTCTGCTGTGAAA+AGG | 0.463378 | 3.3:+88351200 | MS.gene072981:CDS |
| GCTTCCAGAGTGGAAGCAAT+TGG | 0.467373 | 3.3:-88352880 | None:intergenic |
| ACCGAAGCCTGGTACGAACT+TGG | 0.467686 | 3.3:+88351072 | MS.gene072981:CDS |
| GCTGGAGTTGTGGAAACAAT+TGG | 0.469881 | 3.3:-88351402 | None:intergenic |
| AATTCTTCAGCTAGAAATGA+AGG | 0.472110 | 3.3:+88355793 | MS.gene072981:CDS |
| ATGCCTCTTTCCATCTCCAT+TGG | 0.473401 | 3.3:-88356035 | None:intergenic |
| GTACTGTTTGATGTTGTCCC+TGG | 0.476556 | 3.3:+88352817 | MS.gene072981:CDS |
| TGTTCTTGCTTTAGGAGCAA+TGG | 0.477251 | 3.3:+88353977 | MS.gene072981:CDS |
| CCAGCCTTTAGTAGCTGCCA+TGG | 0.483191 | 3.3:+88351420 | MS.gene072981:CDS |
| GAATTAGAAAACACACTAGA+TGG | 0.483789 | 3.3:+88355892 | MS.gene072981:CDS |
| AAATTGTCATTAGTGCGCTC+TGG | 0.488283 | 3.3:+88351764 | MS.gene072981:CDS |
| ACATGTTCTTGAATCTATCA+TGG | 0.490813 | 3.3:+88351174 | MS.gene072981:CDS |
| TTCCATTGTCTGAACGCCAA+TGG | 0.491240 | 3.3:+88356019 | MS.gene072981:CDS |
| CAACCCATGGCAGCTACTAA+AGG | 0.491941 | 3.3:-88351424 | None:intergenic |
| TGCCCTGTCTGCTGTGAAAA+GGG | 0.493064 | 3.3:+88351201 | MS.gene072981:CDS |
| TAGTTCATCTGAAGGATTTG+CGG | 0.493072 | 3.3:+88354045 | MS.gene072981:CDS |
| CTCCATTGGCGTTCAGACAA+TGG | 0.498993 | 3.3:-88356021 | None:intergenic |
| TGTCTGAACGCCAATGGAGA+TGG | 0.510310 | 3.3:+88356025 | MS.gene072981:CDS |
| ATGGGTTGGGATTTATTGGC+CGG | 0.512799 | 3.3:+88351439 | MS.gene072981:CDS |
| TTATCATCCAAGTTCGTACC+AGG | 0.515613 | 3.3:-88351079 | None:intergenic |
| CCATGGCAGCTACTAAAGGC+TGG | 0.524707 | 3.3:-88351420 | None:intergenic |
| TTTGTCTGAAGAGATAGAAG+TGG | 0.530065 | 3.3:+88350985 | MS.gene072981:CDS |
| ATTTCCCGAAGAAGATGCAT+TGG | 0.530706 | 3.3:-88355770 | None:intergenic |
| ATTCTGGCAGATGTCATGTC+TGG | 0.532606 | 3.3:+88351658 | MS.gene072981:intron |
| GCAACCAATTGCTTCCACTC+TGG | 0.534217 | 3.3:+88352876 | MS.gene072981:CDS |
| GCTACTAAAGGCTGGAGTTG+TGG | 0.536888 | 3.3:-88351412 | None:intergenic |
| ACACACTAGATGGTGATGTA+AGG | 0.538392 | 3.3:+88355902 | MS.gene072981:CDS |
| CAGCCTTTAGTAGCTGCCAT+GGG | 0.540778 | 3.3:+88351421 | MS.gene072981:CDS |
| CTACCCATTCAGCTAACTCA+AGG | 0.547713 | 3.3:-88356415 | None:intergenic |
| CCACTCTGGAAGCCTGGAAG+AGG | 0.552966 | 3.3:+88352890 | MS.gene072981:CDS |
| ATTCACACAAGCAACAAAAG+AGG | 0.553766 | 3.3:-88351720 | None:intergenic |
| GATTACTGATAGCATCCAAA+TGG | 0.555742 | 3.3:-88354070 | None:intergenic |
| TATCTGGCAGAACCGAAGCC+TGG | 0.555754 | 3.3:+88351061 | MS.gene072981:CDS |
| ACAATTTCGAATTCCATGAC+TGG | 0.558319 | 3.3:-88351748 | None:intergenic |
| TCACAGGTTGAGTAGCTTGG+AGG | 0.559223 | 3.3:-88356079 | None:intergenic |
| AGATATTGTTCAAATGATGC+AGG | 0.559621 | 3.3:+88348273 | MS.gene072981:CDS |
| CCAGCAGACAGGCCCTCGAG+TGG | 0.565094 | 3.3:+88355929 | MS.gene072981:CDS |
| CAACCCTTGAGTTAGCTGAA+TGG | 0.566029 | 3.3:+88356411 | MS.gene072981:CDS |
| CACAGGTTGAGTAGCTTGGA+GGG | 0.571763 | 3.3:-88356078 | None:intergenic |
| TAAGACATCATCACTGAATG+AGG | 0.575125 | 3.3:+88351099 | MS.gene072981:CDS |
| GAAAATGAAATAACCTTGCG+TGG | 0.579041 | 3.3:-88354095 | None:intergenic |
| TGTAAGGACAACCAGCAGAC+AGG | 0.579881 | 3.3:+88355918 | MS.gene072981:CDS |
| ATTCTTCAGCTAGAAATGAA+GGG | 0.583561 | 3.3:+88355794 | MS.gene072981:CDS |
| ACGCCAATGGAGATGGAAAG+AGG | 0.583575 | 3.3:+88356032 | MS.gene072981:CDS |
| GTTTCATTGAAGAATGGGAA+TGG | 0.584198 | 3.3:+88355971 | MS.gene072981:CDS |
| GTGTGAATTCTGGCCAGTCA+TGG | 0.585691 | 3.3:+88351735 | MS.gene072981:CDS |
| TAGGAGAAGAAGCAGTTCAT+CGG | 0.585750 | 3.3:+88356363 | MS.gene072981:CDS |
| TTCTTCAGCTAGAAATGAAG+GGG | 0.592105 | 3.3:+88355795 | MS.gene072981:CDS |
| TGTGCCACTAGTTCATCTGA+AGG | 0.600872 | 3.3:+88354037 | MS.gene072981:CDS |
| AGATTGACATTCTCCACTCG+AGG | 0.602549 | 3.3:-88355942 | None:intergenic |
| TCTGCTAACTCGATGCATTG+TGG | 0.613542 | 3.3:+88355721 | MS.gene072981:CDS |
| TATCAGTAATCTTCCACGCA+AGG | 0.614522 | 3.3:+88354082 | MS.gene072981:CDS |
| CCTCTTCCAGGCTTCCAGAG+TGG | 0.622873 | 3.3:-88352890 | None:intergenic |
| TGGCCGGTAAAATAGCAGCC+AGG | 0.624005 | 3.3:+88351455 | MS.gene072981:CDS |
| GGCCAATAAATCCCAACCCA+TGG | 0.634109 | 3.3:-88351437 | None:intergenic |
| GATTGACATTCTCCACTCGA+GGG | 0.636278 | 3.3:-88355941 | None:intergenic |
| TACCCATTCAGCTAACTCAA+GGG | 0.639712 | 3.3:-88356414 | None:intergenic |
| GGCCATTATATCAAGAAGTG+AGG | 0.640153 | 3.3:+88348174 | MS.gene072981:CDS |
| GGAGTTGTGGAAACAATTGG+AGG | 0.641709 | 3.3:-88351399 | None:intergenic |
| TCAAAACATTCCATCATCTG+TGG | 0.653352 | 3.3:+88355867 | MS.gene072981:CDS |
| GCGCTCTGGAAAAGAACAAG+TGG | 0.653712 | 3.3:+88351778 | MS.gene072981:CDS |
| TGAAGACGTACAAGAGGACG+CGG | 0.653918 | 3.3:-88351034 | None:intergenic |
| AAATCCTTCAGATGAACTAG+TGG | 0.655455 | 3.3:-88354041 | None:intergenic |
| TTATGGTAATAAATCAGATG+AGG | 0.665709 | 3.3:+88351543 | MS.gene072981:CDS |
| TCTTCAGCTAGAAATGAAGG+GGG | 0.702689 | 3.3:+88355796 | MS.gene072981:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 88348150 | 88356460 | 88348150 | ID=MS.gene072981 |
| chr3.3 | mRNA | 88348150 | 88356460 | 88348150 | ID=MS.gene072981.t1;Parent=MS.gene072981 |
| chr3.3 | exon | 88348150 | 88348294 | 88348150 | ID=MS.gene072981.t1.exon1;Parent=MS.gene072981.t1 |
| chr3.3 | CDS | 88348150 | 88348294 | 88348150 | ID=cds.MS.gene072981.t1;Parent=MS.gene072981.t1 |
| chr3.3 | exon | 88350968 | 88351246 | 88350968 | ID=MS.gene072981.t1.exon2;Parent=MS.gene072981.t1 |
| chr3.3 | CDS | 88350968 | 88351246 | 88350968 | ID=cds.MS.gene072981.t1;Parent=MS.gene072981.t1 |
| chr3.3 | exon | 88351397 | 88351564 | 88351397 | ID=MS.gene072981.t1.exon3;Parent=MS.gene072981.t1 |
| chr3.3 | CDS | 88351397 | 88351564 | 88351397 | ID=cds.MS.gene072981.t1;Parent=MS.gene072981.t1 |
| chr3.3 | exon | 88351668 | 88351883 | 88351668 | ID=MS.gene072981.t1.exon4;Parent=MS.gene072981.t1 |
| chr3.3 | CDS | 88351668 | 88351883 | 88351668 | ID=cds.MS.gene072981.t1;Parent=MS.gene072981.t1 |
| chr3.3 | exon | 88352817 | 88352911 | 88352817 | ID=MS.gene072981.t1.exon5;Parent=MS.gene072981.t1 |
| chr3.3 | CDS | 88352817 | 88352911 | 88352817 | ID=cds.MS.gene072981.t1;Parent=MS.gene072981.t1 |
| chr3.3 | exon | 88353929 | 88354103 | 88353929 | ID=MS.gene072981.t1.exon6;Parent=MS.gene072981.t1 |
| chr3.3 | CDS | 88353929 | 88354103 | 88353929 | ID=cds.MS.gene072981.t1;Parent=MS.gene072981.t1 |
| chr3.3 | exon | 88355673 | 88356097 | 88355673 | ID=MS.gene072981.t1.exon7;Parent=MS.gene072981.t1 |
| chr3.3 | CDS | 88355673 | 88356097 | 88355673 | ID=cds.MS.gene072981.t1;Parent=MS.gene072981.t1 |
| chr3.3 | exon | 88356337 | 88356460 | 88356337 | ID=MS.gene072981.t1.exon8;Parent=MS.gene072981.t1 |
| chr3.3 | CDS | 88356337 | 88356460 | 88356337 | ID=cds.MS.gene072981.t1;Parent=MS.gene072981.t1 |
| Gene Sequence |
| Protein sequence |
