AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07300

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07300.t1 MTR_1g086950 98.311 296 5 0 1 296 1 296 0.0 591
MS.gene07300.t1 MTR_7g109590 51.768 311 122 7 1 295 1 299 4.59e-89 268
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07300.t1 AT2G47360 42.553 235 100 6 63 278 63 281 1.28e-50 169
MS.gene07300.t1 AT1G02570 38.559 236 129 5 64 296 56 278 8.67e-44 150
MS.gene07300.t1 AT1G02575 37.755 196 102 8 75 267 67 245 3.12e-31 118

Find 76 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGAGGAAGTTGAGAATTT+AGG 0.190418 1.2:-65404565 MS.gene07300:CDS
TACATGAAACAACACAAATT+TGG 0.272884 1.2:-65404711 MS.gene07300:CDS
TTTCAAATATGTGTTGGTTT+AGG 0.275769 1.2:-65404853 MS.gene07300:CDS
ATAGTTGAAGAAGAAATCTT+TGG 0.285743 1.2:+65405203 None:intergenic
ATGGATTTGAAGGTAGAATT+TGG 0.286827 1.2:+65405077 None:intergenic
GACGATGGTTCTAGTAGTTT+TGG 0.302357 1.2:-65404778 MS.gene07300:CDS
AGTTGAGAATTTAGGTGTTA+TGG 0.309415 1.2:-65404557 MS.gene07300:CDS
ACAGATTAATGAGGTGGAAT+TGG 0.353558 1.2:-65404383 MS.gene07300:CDS
TACTTTGATCAATGGTTTGA+TGG 0.354298 1.2:-65404965 MS.gene07300:CDS
GCTAAGAGAAGAAAAGATAG+AGG 0.357745 1.2:+65405114 None:intergenic
GTCTTTACATGTCATGGAAA+TGG 0.358547 1.2:-65404891 MS.gene07300:CDS
GAGATTATGGATTGAGAAAG+TGG 0.371004 1.2:-65404629 MS.gene07300:CDS
CTTCTCTTAGCTAGTCTCTC+TGG 0.372938 1.2:-65405102 MS.gene07300:CDS
GTGGTTGTGGCAGGTAGTTT+AGG 0.376947 1.2:-65404610 MS.gene07300:CDS
ATGACATGTAAAGACGAGTT+TGG 0.390360 1.2:+65404899 None:intergenic
GGTATGGTGAGGATTGTTAA+AGG 0.394274 1.2:-65404451 MS.gene07300:CDS
AAAAGAGAAAAGATAAGGTA+AGG 0.399838 1.2:+65405044 None:intergenic
AGGAGGTGGAAGATCCTTGC+AGG 0.405724 1.2:+65405165 None:intergenic
TATTATCTAATTGTCACAAT+TGG 0.405785 1.2:-65404472 MS.gene07300:CDS
AAGGTAAGGGTTGTTGTGAA+TGG 0.409710 1.2:+65405058 None:intergenic
AGTGTAGCTACTTTGATCAA+TGG 0.424175 1.2:-65404973 MS.gene07300:CDS
AGTTTGGAGAAGAATAGAAT+TGG 0.429200 1.2:+65404915 None:intergenic
GAAAAGATAGAGGGAGGAGA+AGG 0.430845 1.2:+65405124 None:intergenic
AAGCAGAATGATATTTCTGT+TGG 0.438907 1.2:-65404737 MS.gene07300:CDS
GTGGGAATAGAAGAATTTGT+TGG 0.445922 1.2:-65404505 MS.gene07300:CDS
TGATTTCTCTATGTAGAAGA+AGG 0.454333 1.2:-65404411 MS.gene07300:CDS
GGATTGTTAAAGGTCTTATG+TGG 0.482472 1.2:-65404441 MS.gene07300:CDS
AATTGGATGAATCATTCATA+AGG 0.483645 1.2:+65404932 None:intergenic
AACAAAGAAAGATGAAATGA+TGG 0.484417 1.2:-65404530 MS.gene07300:CDS
AAAAGATAGAGGGAGGAGAA+GGG 0.494482 1.2:+65405125 None:intergenic
GTTGTTGTGAATGGATTTGA+AGG 0.496977 1.2:+65405067 None:intergenic
GTTTAGGAACATTGTGGTGG+TGG 0.497414 1.2:-65404594 MS.gene07300:CDS
ATGGATTGAGAAAGTGGTTG+TGG 0.498685 1.2:-65404623 MS.gene07300:CDS
TCATTCTACACAAAATGGAT+TGG 0.502402 1.2:-65405236 None:intergenic
TCTAATTGTCACAATTGGTA+TGG 0.503683 1.2:-65404467 MS.gene07300:CDS
GAAAGATGAAATGATGGAGG+TGG 0.504281 1.2:-65404524 MS.gene07300:CDS
GGATAGTGATGGTGAGGAGG+TGG 0.504511 1.2:+65405151 None:intergenic
ACTTTGATCAATGGTTTGAT+GGG 0.504932 1.2:-65404964 MS.gene07300:CDS
GGGTGAGGATAGTGATGGTG+AGG 0.507453 1.2:+65405145 None:intergenic
GTGGTGGATGACACTATATT+TGG 0.509880 1.2:-65404667 MS.gene07300:CDS
AAAGAGAAAAGATAAGGTAA+GGG 0.510747 1.2:+65405045 None:intergenic
AGCAGAATGATATTTCTGTT+GGG 0.516467 1.2:-65404736 MS.gene07300:CDS
ATAGAGGGAGGAGAAGGGTG+AGG 0.523270 1.2:+65405130 None:intergenic
TGAGGATAGTGATGGTGAGG+AGG 0.526694 1.2:+65405148 None:intergenic
ACAAGAACATAGAGAATACA+TGG 0.527333 1.2:+65405006 None:intergenic
TAGAAGAATTTGTTGGATGG+TGG 0.527444 1.2:-65404498 MS.gene07300:CDS
AAACTCGTCTTTACATGTCA+TGG 0.528069 1.2:-65404897 MS.gene07300:CDS
ATAGCAAAAGAGAAAAGATA+AGG 0.540841 1.2:+65405039 None:intergenic
GATGACACTATATTTGGTGT+TGG 0.542896 1.2:-65404661 MS.gene07300:CDS
ATTGAAGCAAGCATTGCAGC+TGG 0.549729 1.2:-65404829 MS.gene07300:CDS
AAAGATGAAATGATGGAGGT+GGG 0.557012 1.2:-65404523 MS.gene07300:CDS
CAAAGTAGCTACACTTACAA+TGG 0.557980 1.2:+65404981 None:intergenic
ATTGAGAAAGTGGTTGTGGC+AGG 0.560585 1.2:-65404619 MS.gene07300:CDS
GAGAAGGGTGAGGATAGTGA+TGG 0.583678 1.2:+65405140 None:intergenic
AGAGAAGAAAAGATAGAGGG+AGG 0.590383 1.2:+65405118 None:intergenic
TTGTCACAATTGGTATGGTG+AGG 0.590458 1.2:-65404462 MS.gene07300:CDS
AAAGAAAGATGAAATGATGG+AGG 0.600117 1.2:-65404527 MS.gene07300:CDS
CTAAGAGAAGAAAAGATAGA+GGG 0.601150 1.2:+65405115 None:intergenic
TTGCTATCTACACCCCTGCA+AGG 0.606162 1.2:-65405179 MS.gene07300:CDS
GGTAACACAGATTAATGAGG+TGG 0.609717 1.2:-65404389 MS.gene07300:CDS
GATTTCTCTATGTAGAAGAA+GGG 0.614319 1.2:-65404410 MS.gene07300:CDS
AATATAGTGTCATCCACCAC+TGG 0.619508 1.2:+65404670 None:intergenic
GAATAGAAGAATTTGTTGGA+TGG 0.624191 1.2:-65404501 MS.gene07300:CDS
GATTGTTAAAGGTCTTATGT+GGG 0.625679 1.2:-65404440 MS.gene07300:CDS
AACATAGAGAATACATGGGG+AGG 0.632641 1.2:+65405011 None:intergenic
AAGGGTAACACAGATTAATG+AGG 0.632667 1.2:-65404392 MS.gene07300:CDS
GGAGGTGGAAGATCCTTGCA+GGG 0.633817 1.2:+65405166 None:intergenic
GTGGTGGTGGAAGTTGAGAG+AGG 0.639795 1.2:-65404581 MS.gene07300:CDS
CAAGAACATAGAGAATACAT+GGG 0.640474 1.2:+65405007 None:intergenic
AGTTGTTGTGAGACCAGTGG+TGG 0.655710 1.2:-65404683 MS.gene07300:CDS
GTAGTTTAGGAACATTGTGG+TGG 0.662467 1.2:-65404597 MS.gene07300:CDS
CAGGTAGTTTAGGAACATTG+TGG 0.681497 1.2:-65404600 MS.gene07300:CDS
GAGGTGGAAGATCCTTGCAG+GGG 0.700426 1.2:+65405167 None:intergenic
AAGAGTTGTTGTGAGACCAG+TGG 0.721375 1.2:-65404686 MS.gene07300:CDS
AAGAACATAGAGAATACATG+GGG 0.725668 1.2:+65405008 None:intergenic
ACTGAGTCAGAATGATGACA+AGG 0.757755 1.2:-65404359 MS.gene07300:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTATCTAATTGTCACAAT+TGG - chr1.2:65405104-65405123 MS.gene07300:CDS 20.0%
! AAAAGAGAAAAGATAAGGTA+AGG + chr1.2:65404535-65404554 None:intergenic 25.0%
! AAAGAGAAAAGATAAGGTAA+GGG + chr1.2:65404534-65404553 None:intergenic 25.0%
! AACAAAGAAAGATGAAATGA+TGG - chr1.2:65405046-65405065 MS.gene07300:CDS 25.0%
! AATTGGATGAATCATTCATA+AGG + chr1.2:65404647-65404666 None:intergenic 25.0%
! ATAGCAAAAGAGAAAAGATA+AGG + chr1.2:65404540-65404559 None:intergenic 25.0%
! ATAGTTGAAGAAGAAATCTT+TGG + chr1.2:65404376-65404395 None:intergenic 25.0%
! TACATGAAACAACACAAATT+TGG - chr1.2:65404865-65404884 MS.gene07300:CDS 25.0%
!!! TTCACTTTTCAAATATGTGT+TGG - chr1.2:65404717-65404736 MS.gene07300:CDS 25.0%
!!! TTTCAAATATGTGTTGGTTT+AGG - chr1.2:65404723-65404742 MS.gene07300:CDS 25.0%
AAAGAAAGATGAAATGATGG+AGG - chr1.2:65405049-65405068 MS.gene07300:CDS 30.0%
AAGAACATAGAGAATACATG+GGG + chr1.2:65404571-65404590 None:intergenic 30.0%
ACAAGAACATAGAGAATACA+TGG + chr1.2:65404573-65404592 None:intergenic 30.0%
ACTTTGATCAATGGTTTGAT+GGG - chr1.2:65404612-65404631 MS.gene07300:CDS 30.0%
AGTTGAGAATTTAGGTGTTA+TGG - chr1.2:65405019-65405038 MS.gene07300:CDS 30.0%
AGTTTGGAGAAGAATAGAAT+TGG + chr1.2:65404664-65404683 None:intergenic 30.0%
ATGGATTTGAAGGTAGAATT+TGG + chr1.2:65404502-65404521 None:intergenic 30.0%
CAAGAACATAGAGAATACAT+GGG + chr1.2:65404572-65404591 None:intergenic 30.0%
CTAAGAGAAGAAAAGATAGA+GGG + chr1.2:65404464-65404483 None:intergenic 30.0%
GATTTCTCTATGTAGAAGAA+GGG - chr1.2:65405166-65405185 MS.gene07300:CDS 30.0%
TACTTTGATCAATGGTTTGA+TGG - chr1.2:65404611-65404630 MS.gene07300:CDS 30.0%
TCTAATTGTCACAATTGGTA+TGG - chr1.2:65405109-65405128 MS.gene07300:CDS 30.0%
TGATTTCTCTATGTAGAAGA+AGG - chr1.2:65405165-65405184 MS.gene07300:CDS 30.0%
TTGGAAAAAGAGAGAGATTA+TGG - chr1.2:65404934-65404953 MS.gene07300:CDS 30.0%
! AAGCAGAATGATATTTCTGT+TGG - chr1.2:65404839-65404858 MS.gene07300:CDS 30.0%
! AGCAGAATGATATTTCTGTT+GGG - chr1.2:65404840-65404859 MS.gene07300:CDS 30.0%
! GATTGTTAAAGGTCTTATGT+GGG - chr1.2:65405136-65405155 MS.gene07300:CDS 30.0%
!! GAATAGAAGAATTTGTTGGA+TGG - chr1.2:65405075-65405094 MS.gene07300:CDS 30.0%
AAACTCGTCTTTACATGTCA+TGG - chr1.2:65404679-65404698 MS.gene07300:CDS 35.0%
AAGGGTAACACAGATTAATG+AGG - chr1.2:65405184-65405203 MS.gene07300:CDS 35.0%
ACAGATTAATGAGGTGGAAT+TGG - chr1.2:65405193-65405212 MS.gene07300:CDS 35.0%
AGAGAGGAAGTTGAGAATTT+AGG - chr1.2:65405011-65405030 MS.gene07300:CDS 35.0%
ATCGTCAAAAACATCGTTGT+CGG + chr1.2:65404784-65404803 None:intergenic 35.0%
ATGACATGTAAAGACGAGTT+TGG + chr1.2:65404680-65404699 None:intergenic 35.0%
CAAAGTAGCTACACTTACAA+TGG + chr1.2:65404598-65404617 None:intergenic 35.0%
GCTAAGAGAAGAAAAGATAG+AGG + chr1.2:65404465-65404484 None:intergenic 35.0%
GTCTTTACATGTCATGGAAA+TGG - chr1.2:65404685-65404704 MS.gene07300:CDS 35.0%
GTGGGAATAGAAGAATTTGT+TGG - chr1.2:65405071-65405090 MS.gene07300:CDS 35.0%
! AAAGATGAAATGATGGAGGT+GGG - chr1.2:65405053-65405072 MS.gene07300:CDS 35.0%
! AGTGTAGCTACTTTGATCAA+TGG - chr1.2:65404603-65404622 MS.gene07300:CDS 35.0%
! GAGATTATGGATTGAGAAAG+TGG - chr1.2:65404947-65404966 MS.gene07300:CDS 35.0%
! GGATTGTTAAAGGTCTTATG+TGG - chr1.2:65405135-65405154 MS.gene07300:CDS 35.0%
! GTTGTTGTGAATGGATTTGA+AGG + chr1.2:65404512-65404531 None:intergenic 35.0%
!! GATGACACTATATTTGGTGT+TGG - chr1.2:65404915-65404934 MS.gene07300:CDS 35.0%
!! TAGAAGAATTTGTTGGATGG+TGG - chr1.2:65405078-65405097 MS.gene07300:CDS 35.0%
AAAAGATAGAGGGAGGAGAA+GGG + chr1.2:65404454-65404473 None:intergenic 40.0%
AACATAGAGAATACATGGGG+AGG + chr1.2:65404568-65404587 None:intergenic 40.0%
AAGGTAAGGGTTGTTGTGAA+TGG + chr1.2:65404521-65404540 None:intergenic 40.0%
AATATAGTGTCATCCACCAC+TGG + chr1.2:65404909-65404928 None:intergenic 40.0%
ACTGAGTCAGAATGATGACA+AGG - chr1.2:65405217-65405236 MS.gene07300:CDS 40.0%
AGAGAAGAAAAGATAGAGGG+AGG + chr1.2:65404461-65404480 None:intergenic 40.0%
CAGGTAGTTTAGGAACATTG+TGG - chr1.2:65404976-65404995 MS.gene07300:CDS 40.0%
GGTAACACAGATTAATGAGG+TGG - chr1.2:65405187-65405206 MS.gene07300:CDS 40.0%
GGTATGGTGAGGATTGTTAA+AGG - chr1.2:65405125-65405144 MS.gene07300:CDS 40.0%
GTAGTTTAGGAACATTGTGG+TGG - chr1.2:65404979-65404998 MS.gene07300:CDS 40.0%
TTGTCACAATTGGTATGGTG+AGG - chr1.2:65405114-65405133 MS.gene07300:CDS 40.0%
! ATGGATTGAGAAAGTGGTTG+TGG - chr1.2:65404953-65404972 MS.gene07300:CDS 40.0%
! GAAAGATGAAATGATGGAGG+TGG - chr1.2:65405052-65405071 MS.gene07300:CDS 40.0%
!! GACAACGATGTTTTTGACGA+TGG - chr1.2:65404783-65404802 MS.gene07300:CDS 40.0%
!! GTGGTGGATGACACTATATT+TGG - chr1.2:65404909-65404928 MS.gene07300:CDS 40.0%
!!! GACGATGGTTCTAGTAGTTT+TGG - chr1.2:65404798-65404817 MS.gene07300:CDS 40.0%
AAGAGTTGTTGTGAGACCAG+TGG - chr1.2:65404890-65404909 MS.gene07300:CDS 45.0%
ATTGAGAAAGTGGTTGTGGC+AGG - chr1.2:65404957-65404976 MS.gene07300:CDS 45.0%
GAAAAGATAGAGGGAGGAGA+AGG + chr1.2:65404455-65404474 None:intergenic 45.0%
GTTTAGGAACATTGTGGTGG+TGG - chr1.2:65404982-65405001 MS.gene07300:CDS 45.0%
! ATTGAAGCAAGCATTGCAGC+TGG - chr1.2:65404747-65404766 MS.gene07300:CDS 45.0%
! CTTCTCTTAGCTAGTCTCTC+TGG - chr1.2:65404474-65404493 MS.gene07300:CDS 45.0%
AGTTGTTGTGAGACCAGTGG+TGG - chr1.2:65404893-65404912 MS.gene07300:CDS 50.0%
GAGAAGGGTGAGGATAGTGA+TGG + chr1.2:65404439-65404458 None:intergenic 50.0%
GTGGTTGTGGCAGGTAGTTT+AGG - chr1.2:65404966-65404985 MS.gene07300:CDS 50.0%
TTGCTATCTACACCCCTGCA+AGG - chr1.2:65404397-65404416 MS.gene07300:CDS 50.0%
! TGAGGATAGTGATGGTGAGG+AGG + chr1.2:65404431-65404450 None:intergenic 50.0%
AGGAGGTGGAAGATCCTTGC+AGG + chr1.2:65404414-65404433 None:intergenic 55.0%
ATAGAGGGAGGAGAAGGGTG+AGG + chr1.2:65404449-65404468 None:intergenic 55.0%
GAGGTGGAAGATCCTTGCAG+GGG + chr1.2:65404412-65404431 None:intergenic 55.0%
GGAGGTGGAAGATCCTTGCA+GGG + chr1.2:65404413-65404432 None:intergenic 55.0%
! GGATAGTGATGGTGAGGAGG+TGG + chr1.2:65404428-65404447 None:intergenic 55.0%
! GGGTGAGGATAGTGATGGTG+AGG + chr1.2:65404434-65404453 None:intergenic 55.0%
!! GTGGTGGTGGAAGTTGAGAG+AGG - chr1.2:65404995-65405014 MS.gene07300:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 65404354 65405244 65404354 ID=MS.gene07300
chr1.2 mRNA 65404354 65405244 65404354 ID=MS.gene07300.t1;Parent=MS.gene07300
chr1.2 exon 65404354 65405244 65404354 ID=MS.gene07300.t1.exon1;Parent=MS.gene07300.t1
chr1.2 CDS 65404354 65405244 65404354 ID=cds.MS.gene07300.t1;Parent=MS.gene07300.t1
Gene Sequence

>MS.gene07300

ATGGATTGGCAGAAAAAATCCAAAGATTTCTTCTTCAACTATATTGCTATCTACACCCCTGCAAGGATCTTCCACCTCCTCACCATCACTATCCTCACCCTTCTCCTCCCTCTATCTTTTCTTCTCTTAGCTAGTCTCTCTGGTGCCAAATTCTACCTTCAAATCCATTCACAACAACCCTTACCTTATCTTTTCTCTTTTGCTATTCACACCTCCCCATGTATTCTCTATGTTCTTGTTTCCATTGTAAGTGTAGCTACTTTGATCAATGGTTTGATGGGAAAAATCAACCTTATGAATGATTCATCCAATTCTATTCTTCTCCAAACTCGTCTTTACATGTCATGGAAATGGATTCTTCTTTTCACTTTTCAAATATGTGTTGGTTTAGGAATTGAAGCAAGCATTGCAGCTGGAGTTTTCGACTCCGACAACGATGTTTTTGACGATGGTTCTAGTAGTTTTGGTGTTGAGAGAAGCTTCATAAGCAGAATGATATTTCTGTTGGGACTACATGAAACAACACAAATTTGGTCAAGAGTTGTTGTGAGACCAGTGGTGGATGACACTATATTTGGTGTTGGAAAAAGAGAGAGATTATGGATTGAGAAAGTGGTTGTGGCAGGTAGTTTAGGAACATTGTGGTGGTGGAAGTTGAGAGAGGAAGTTGAGAATTTAGGTGTTATGGCTGAAACAAAGAAAGATGAAATGATGGAGGTGGGAATAGAAGAATTTGTTGGATGGTGGTTATATTATCTAATTGTCACAATTGGTATGGTGAGGATTGTTAAAGGTCTTATGTGGGTGTTCATGATTTCTCTATGTAGAAGAAGGGTAACACAGATTAATGAGGTGGAATTGGAACTGAGTCAGAATGATGACAAGGTGTAA
Protein sequence

>MS.gene07300.t1

MDWQKKSKDFFFNYIAIYTPARIFHLLTITILTLLLPLSFLLLASLSGAKFYLQIHSQQPLPYLFSFAIHTSPCILYVLVSIVSVATLINGLMGKINLMNDSSNSILLQTRLYMSWKWILLFTFQICVGLGIEASIAAGVFDSDNDVFDDGSSSFGVERSFISRMIFLLGLHETTQIWSRVVVRPVVDDTIFGVGKRERLWIEKVVVAGSLGTLWWWKLREEVENLGVMAETKKDEMMEVGIEEFVGWWLYYLIVTIGMVRIVKGLMWVFMISLCRRRVTQINEVELELSQNDDKV