Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073061.t1 | AES73844.1 | 94.9 | 118 | 5 | 1 | 1 | 118 | 1 | 117 | 1.40E-58 | 235.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073061.t1 | G7J2X5 | 94.9 | 118 | 5 | 1 | 1 | 118 | 1 | 117 | 1.0e-58 | 235.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05985 | MS.gene073061 | -0.807458 | 5.01E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073061.t1 | MTR_3g109430 | 94.915 | 118 | 5 | 1 | 1 | 118 | 1 | 117 | 1.09e-72 | 212 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATCGACCAACTAAATATTT+AGG | 0.285504 | 3.3:-89992017 | None:intergenic |
| AAACAGTATGGCAGAAGATT+TGG | 0.311355 | 3.3:+89991802 | MS.gene073061:CDS |
| AACGAACGAGGGAGATTATC+TGG | 0.376163 | 3.3:-89991957 | None:intergenic |
| TTACCACCTAAATATTTAGT+TGG | 0.429402 | 3.3:+89992011 | MS.gene073061:CDS |
| CCATCTTCAACTATAGTAGA+AGG | 0.451427 | 3.3:-89991870 | None:intergenic |
| TTGCTACCATCATAGTTGCT+TGG | 0.451500 | 3.3:-89991777 | None:intergenic |
| CTCGGTATGCTGATCCATCT+TGG | 0.452007 | 3.3:+89991985 | MS.gene073061:CDS |
| GATATGAAGAAGTACATGTT+TGG | 0.463772 | 3.3:+89991687 | MS.gene073061:CDS |
| TCAAGAAGAGAAGCAAGGTT+AGG | 0.500253 | 3.3:+89991711 | MS.gene073061:CDS |
| TACTCAATGAACTTTGATCA+TGG | 0.501867 | 3.3:+89991918 | MS.gene073061:CDS |
| ATGAACTTTGATCATGGAGC+AGG | 0.506680 | 3.3:+89991924 | MS.gene073061:CDS |
| TCCCTCGTTCGTTCTCAGCT+CGG | 0.509690 | 3.3:+89991967 | MS.gene073061:CDS |
| CCTTCTACTATAGTTGAAGA+TGG | 0.515448 | 3.3:+89991870 | MS.gene073061:CDS |
| GAAGAGAAGCAAGGTTAGGT+AGG | 0.539622 | 3.3:+89991715 | MS.gene073061:CDS |
| TGATCATGGAGCAGGTTGGA+TGG | 0.546024 | 3.3:+89991932 | MS.gene073061:CDS |
| ATTTCACCAAGCAACTATGA+TGG | 0.581383 | 3.3:+89991771 | MS.gene073061:CDS |
| TTAGGTGGTAAGATCCAAGA+TGG | 0.602511 | 3.3:-89991999 | None:intergenic |
| CGACCAACTAAATATTTAGG+TGG | 0.616235 | 3.3:-89992014 | None:intergenic |
| TCAACTATAGTAGAAGGAGA+AGG | 0.616257 | 3.3:-89991864 | None:intergenic |
| TACCGAGCTGAGAACGAACG+AGG | 0.628756 | 3.3:-89991969 | None:intergenic |
| ACTTTGATCATGGAGCAGGT+TGG | 0.656134 | 3.3:+89991928 | MS.gene073061:CDS |
| AGGTAGGAGCTACACAACAA+AGG | 0.677040 | 3.3:+89991731 | MS.gene073061:CDS |
| TTGGCTCAAGAAGAGAAGCA+AGG | 0.681319 | 3.3:+89991706 | MS.gene073061:CDS |
| ACCGAGCTGAGAACGAACGA+GGG | 0.691206 | 3.3:-89991968 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTACCACCTAAATATTTAGT+TGG | + | chr3.3:89992011-89992030 | MS.gene073061:CDS | 25.0% |
| ATGGTAGCAAAAAAACAGTA+TGG | + | chr3.3:89991790-89991809 | MS.gene073061:CDS | 30.0% | |
| CATCGACCAACTAAATATTT+AGG | - | chr3.3:89992020-89992039 | None:intergenic | 30.0% | |
| TACTCAATGAACTTTGATCA+TGG | + | chr3.3:89991918-89991937 | MS.gene073061:CDS | 30.0% | |
| ! | GATATGAAGAAGTACATGTT+TGG | + | chr3.3:89991687-89991706 | MS.gene073061:CDS | 30.0% |
| AAACAGTATGGCAGAAGATT+TGG | + | chr3.3:89991802-89991821 | MS.gene073061:CDS | 35.0% | |
| ATTTCACCAAGCAACTATGA+TGG | + | chr3.3:89991771-89991790 | MS.gene073061:CDS | 35.0% | |
| CCATCTTCAACTATAGTAGA+AGG | - | chr3.3:89991873-89991892 | None:intergenic | 35.0% | |
| CCTTCTACTATAGTTGAAGA+TGG | + | chr3.3:89991870-89991889 | MS.gene073061:CDS | 35.0% | |
| CGACCAACTAAATATTTAGG+TGG | - | chr3.3:89992017-89992036 | None:intergenic | 35.0% | |
| TCAACTATAGTAGAAGGAGA+AGG | - | chr3.3:89991867-89991886 | None:intergenic | 35.0% | |
| ATGAACTTTGATCATGGAGC+AGG | + | chr3.3:89991924-89991943 | MS.gene073061:CDS | 40.0% | |
| TCAAGAAGAGAAGCAAGGTT+AGG | + | chr3.3:89991711-89991730 | MS.gene073061:CDS | 40.0% | |
| TTAGGTGGTAAGATCCAAGA+TGG | - | chr3.3:89992002-89992021 | None:intergenic | 40.0% | |
| TTGCTACCATCATAGTTGCT+TGG | - | chr3.3:89991780-89991799 | None:intergenic | 40.0% | |
| AACGAACGAGGGAGATTATC+TGG | - | chr3.3:89991960-89991979 | None:intergenic | 45.0% | |
| ACTTTGATCATGGAGCAGGT+TGG | + | chr3.3:89991928-89991947 | MS.gene073061:CDS | 45.0% | |
| AGGTAGGAGCTACACAACAA+AGG | + | chr3.3:89991731-89991750 | MS.gene073061:CDS | 45.0% | |
| GAAGAGAAGCAAGGTTAGGT+AGG | + | chr3.3:89991715-89991734 | MS.gene073061:CDS | 45.0% | |
| TTGGCTCAAGAAGAGAAGCA+AGG | + | chr3.3:89991706-89991725 | MS.gene073061:CDS | 45.0% | |
| CTCGGTATGCTGATCCATCT+TGG | + | chr3.3:89991985-89992004 | MS.gene073061:CDS | 50.0% | |
| ! | TGATCATGGAGCAGGTTGGA+TGG | + | chr3.3:89991932-89991951 | MS.gene073061:CDS | 50.0% |
| ACCGAGCTGAGAACGAACGA+GGG | - | chr3.3:89991971-89991990 | None:intergenic | 55.0% | |
| TACCGAGCTGAGAACGAACG+AGG | - | chr3.3:89991972-89991991 | None:intergenic | 55.0% | |
| TCCCTCGTTCGTTCTCAGCT+CGG | + | chr3.3:89991967-89991986 | MS.gene073061:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 89991684 | 89992040 | 89991684 | ID=MS.gene073061 |
| chr3.3 | mRNA | 89991684 | 89992040 | 89991684 | ID=MS.gene073061.t1;Parent=MS.gene073061 |
| chr3.3 | exon | 89991684 | 89992040 | 89991684 | ID=MS.gene073061.t1.exon1;Parent=MS.gene073061.t1 |
| chr3.3 | CDS | 89991684 | 89992040 | 89991684 | ID=cds.MS.gene073061.t1;Parent=MS.gene073061.t1 |
| Gene Sequence |
| Protein sequence |
