AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene073242

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073242.t1 MTR_0017s0100 97.612 335 8 0 1 335 35 369 0.0 685
MS.gene073242.t1 MTR_4g038440 86.269 335 46 0 1 335 35 369 0.0 613
MS.gene073242.t1 MTR_0079s0070 86.269 335 46 0 1 335 1 335 0.0 612
MS.gene073242.t1 MTR_4g038440 84.746 236 36 0 1 236 35 270 3.43e-150 423
MS.gene073242.t1 MTR_3g083620 39.521 334 189 6 4 332 34 359 6.04e-82 253
MS.gene073242.t1 MTR_5g074600 41.056 341 189 8 1 334 24 359 5.56e-74 233
MS.gene073242.t1 MTR_5g074680 38.576 337 197 7 3 333 27 359 7.81e-71 224
MS.gene073242.t1 MTR_7g118300 39.244 344 191 9 2 333 37 374 1.23e-70 224
MS.gene073242.t1 MTR_3g092900 37.500 336 188 11 10 333 36 361 1.33e-69 221
MS.gene073242.t1 MTR_8g024160 43.377 302 149 10 30 315 14 309 6.74e-69 218
MS.gene073242.t1 MTR_7g118320 37.383 321 182 11 7 315 30 343 7.85e-68 217
MS.gene073242.t1 MTR_5g074580 37.278 338 202 6 3 334 27 360 1.28e-65 211
MS.gene073242.t1 MTR_5g098170 36.562 320 189 8 10 320 35 349 1.73e-64 208
MS.gene073242.t1 MTR_1g076940 37.209 344 200 9 1 334 24 361 1.11e-62 203
MS.gene073242.t1 MTR_8g068750 35.542 332 189 10 9 324 47 369 6.67e-61 199
MS.gene073242.t1 MTR_7g014510 35.277 343 204 8 1 334 24 357 2.45e-59 194
MS.gene073242.t1 MTR_5g074610 35.693 339 181 8 1 334 20 326 4.28e-58 191
MS.gene073242.t1 MTR_4g079160 34.795 342 202 7 3 334 25 355 6.61e-58 191
MS.gene073242.t1 MTR_5g074540 34.808 339 191 7 3 334 26 341 1.47e-56 187
MS.gene073242.t1 MTR_7g014570 35.312 337 201 8 4 334 27 352 4.29e-56 186
MS.gene073242.t1 MTR_7g024520 35.542 332 202 7 3 331 25 347 5.67e-56 186
MS.gene073242.t1 MTR_6g078850 34.268 321 192 10 8 315 50 364 6.51e-56 189
MS.gene073242.t1 MTR_7g024500 34.743 331 204 7 4 331 26 347 1.33e-55 185
MS.gene073242.t1 MTR_0591s0010 35.220 318 187 11 10 315 48 358 1.45e-55 186
MS.gene073242.t1 MTR_8g068870 34.277 318 192 9 9 315 33 344 3.49e-55 184
MS.gene073242.t1 MTR_3g021440 35.625 320 192 10 10 321 43 356 1.33e-54 183
MS.gene073242.t1 MTR_8g068690 33.956 321 195 9 10 319 47 361 2.91e-54 182
MS.gene073242.t1 MTR_4g088340 33.923 339 213 7 1 334 1 333 5.33e-53 177
MS.gene073242.t1 MTR_4g088345 33.235 340 214 7 1 334 24 356 2.75e-52 176
MS.gene073242.t1 MTR_4g088200 32.353 340 218 8 3 334 26 361 5.14e-51 173
MS.gene073242.t1 MTR_7g012020 34.568 324 192 11 10 321 40 355 7.31e-51 173
MS.gene073242.t1 MTR_7g012040 33.731 335 204 12 10 333 40 367 1.21e-50 172
MS.gene073242.t1 MTR_4g033150 34.604 341 209 8 4 334 27 363 1.81e-50 172
MS.gene073242.t1 MTR_2g055940 35.119 336 198 13 10 333 35 362 1.97e-50 172
MS.gene073242.t1 MTR_1g036510 33.746 323 196 10 10 320 52 368 2.26e-50 172
MS.gene073242.t1 MTR_3g096050 34.139 331 204 9 9 331 31 355 6.78e-50 170
MS.gene073242.t1 MTR_7g012090 32.353 340 208 10 10 333 41 374 1.88e-49 169
MS.gene073242.t1 MTR_3g021430 33.125 320 200 9 10 321 30 343 3.18e-49 168
MS.gene073242.t1 MTR_7g012070 32.941 340 206 10 10 333 41 374 4.71e-49 168
MS.gene073242.t1 MTR_1g036460 33.746 323 196 10 10 320 38 354 5.76e-49 168
MS.gene073242.t1 MTR_1g036490 32.544 338 208 11 10 333 38 369 6.61e-49 168
MS.gene073242.t1 MTR_7g011990 33.851 322 194 11 10 321 41 353 9.21e-49 167
MS.gene073242.t1 MTR_4g033015 34.211 342 211 8 3 334 26 363 2.15e-48 166
MS.gene073242.t1 MTR_2g072790 34.242 330 206 9 8 331 27 351 2.36e-48 166
MS.gene073242.t1 MTR_1g096310 34.862 327 187 12 10 320 41 357 3.45e-48 166
MS.gene073242.t1 MTR_4g033200 33.918 342 212 8 3 334 26 363 1.71e-47 164
MS.gene073242.t1 MTR_7g011900 33.230 322 197 10 10 320 41 355 7.67e-47 162
MS.gene073242.t1 MTR_4g088190 34.111 343 211 8 3 334 26 364 9.35e-46 159
MS.gene073242.t1 MTR_8g068570 45.029 171 91 3 133 302 150 318 8.03e-45 156
MS.gene073242.t1 MTR_8g068730 32.087 321 163 9 9 315 47 326 8.53e-43 151
MS.gene073242.t1 MTR_4g033170 32.353 340 214 8 3 334 26 357 3.83e-41 147
MS.gene073242.t1 MTR_3g096070 31.707 328 193 9 9 331 31 332 4.20e-41 146
MS.gene073242.t1 MTR_4g033085 32.047 337 219 7 3 334 26 357 1.92e-38 140
MS.gene073242.t1 MTR_8g068690 32.083 240 148 7 10 239 47 281 4.83e-35 129
MS.gene073242.t1 MTR_8g068740 32.031 256 121 7 8 250 46 261 3.20e-33 124
MS.gene073242.t1 MTR_1g050220 29.217 332 169 12 10 329 21 298 3.96e-33 124
MS.gene073242.t1 MTR_8g068600 33.047 233 141 7 15 237 27 254 8.16e-33 123
MS.gene073242.t1 MTR_3g096050 34.188 234 143 6 9 236 31 259 1.38e-32 122
MS.gene073242.t1 MTR_7g012020 34.177 237 143 7 9 236 39 271 8.38e-32 121
MS.gene073242.t1 MTR_1g076930 32.479 234 149 6 8 236 8 237 5.36e-29 112
MS.gene073242.t1 MTR_1g036510 31.646 237 149 6 10 236 52 285 7.00e-28 110
MS.gene073242.t1 MTR_8g068620 46.018 113 60 1 127 239 2 113 7.87e-27 102
MS.gene073242.t1 MTR_4g025570 43.750 96 54 0 238 333 122 217 8.52e-25 100
MS.gene073242.t1 MTR_7g012020 32.558 215 132 7 10 215 40 250 2.31e-24 100
MS.gene073242.t1 MTR_0794s0020 30.088 226 148 5 4 222 27 249 1.09e-21 92.8
MS.gene073242.t1 MTR_1g036510 29.630 216 139 6 10 215 52 264 2.79e-20 89.4
MS.gene073242.t1 MTR_8g068760 41.304 92 50 3 236 324 135 225 2.81e-14 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073242.t1 AT5G54160 37.188 320 190 6 8 321 34 348 2.49e-73 231
MS.gene073242.t1 AT4G35160 36.994 346 197 6 4 333 40 380 6.94e-66 213
MS.gene073242.t1 AT1G77520 36.982 338 192 9 10 331 46 378 5.13e-63 205
MS.gene073242.t1 AT1G51990 32.941 340 208 10 8 334 30 362 2.65e-56 187
MS.gene073242.t1 AT1G51990 32.647 340 209 10 8 334 30 362 9.60e-54 180
MS.gene073242.t1 AT4G35150 31.765 340 180 6 4 333 26 323 1.18e-53 179
MS.gene073242.t1 AT1G63140 36.176 340 196 9 8 331 44 378 1.81e-53 180
MS.gene073242.t1 AT1G63140 36.176 340 196 9 8 331 64 398 2.30e-53 180
MS.gene073242.t1 AT1G77530 35.882 340 197 10 8 331 44 378 7.51e-53 179
MS.gene073242.t1 AT1G33030 35.329 334 195 12 10 331 23 347 2.50e-52 176
MS.gene073242.t1 AT5G53810 33.431 341 205 9 10 333 42 377 7.16e-50 171
MS.gene073242.t1 AT5G37170 36.481 233 141 5 104 331 1 231 4.94e-47 159
MS.gene073242.t1 AT1G62900 44.279 201 108 3 133 331 4 202 7.75e-47 157
MS.gene073242.t1 AT1G21130 36.335 322 180 12 10 315 42 354 1.01e-46 162
MS.gene073242.t1 AT1G21100 36.025 322 181 11 10 315 42 354 2.15e-46 161
MS.gene073242.t1 AT3G53140 33.433 335 207 10 8 331 27 356 3.38e-46 160
MS.gene073242.t1 AT5G37170 32.468 308 177 9 29 331 50 331 3.42e-46 160
MS.gene073242.t1 AT1G21120 35.404 322 183 11 10 315 42 354 9.33e-45 157
MS.gene073242.t1 AT1G21110 34.146 328 191 11 10 321 42 360 3.33e-44 155
MS.gene073242.t1 AT1G21120 35.404 322 183 11 10 315 90 402 3.39e-44 157
MS.gene073242.t1 AT1G76790 34.604 341 192 12 11 331 33 362 6.90e-43 152
MS.gene073242.t1 AT5G37170 40.110 182 104 4 153 331 4 183 6.98e-42 144
MS.gene073242.t1 AT1G33030 34.599 237 138 9 10 236 23 252 8.82e-28 109
MS.gene073242.t1 AT1G33030 34.599 237 138 9 10 236 23 252 1.68e-27 108
MS.gene073242.t1 AT1G63140 33.610 241 142 7 10 236 66 302 6.04e-27 108
MS.gene073242.t1 AT1G21130 34.025 241 137 10 10 236 42 274 9.63e-22 94.0
MS.gene073242.t1 AT1G63140 31.818 220 132 7 10 215 66 281 2.06e-18 84.3
MS.gene073242.t1 AT1G77530 32.883 222 131 8 8 215 44 261 1.18e-17 82.0

Find 70 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAAACAACTCATTGAATTT+TGG 0.113417 4.3:+55071633 None:intergenic
CATCAACCAATGTTTGAATT+TGG 0.182200 4.3:+55071541 None:intergenic
ATTTCACTCATAAAGTTAAA+AGG 0.207818 4.3:+55071735 None:intergenic
AGAGCTTACCCTCACATTAA+AGG 0.236738 4.3:-55071480 MS.gene073242:CDS
GATGAACCATTTACTGATAT+TGG 0.243931 4.3:-55070683 MS.gene073242:CDS
GTTGATGTTGGTGGTGGTAT+TGG 0.262573 4.3:-55071525 MS.gene073242:CDS
TTAAAAGGGTTCATATGAAT+TGG 0.290679 4.3:+55071750 None:intergenic
AAAATGGCACTGCTTTCTTT+AGG 0.298524 4.3:-55071695 MS.gene073242:CDS
GCTATGCCAATATCAGTAAA+TGG 0.309320 4.3:+55070677 None:intergenic
GCTTGACACGTGCATCAAAA+TGG 0.313818 4.3:-55071827 MS.gene073242:CDS
CCACACTAGCCATTTCAAAT+TGG 0.317958 4.3:+55071658 None:intergenic
CTTAAAAGTAAAGTGGAATC+AGG 0.331497 4.3:+55071921 None:intergenic
ATTCTGCACAATTTGAGTTA+AGG 0.365186 4.3:+55071962 None:intergenic
TATGATTTATGAATTTAGAT+TGG 0.372355 4.3:+55070512 None:intergenic
GTAAATGGTTCATCTCCTTC+TGG 0.373473 4.3:+55070692 None:intergenic
TAAAGATATTGATGAATTGT+AGG 0.389686 4.3:-55070769 MS.gene073242:CDS
AACTGGAAAGGTGATAATAT+TGG 0.390122 4.3:-55070729 MS.gene073242:CDS
TAAAAGGGTTCATATGAATT+GGG 0.395062 4.3:+55071751 None:intergenic
GTGTTTAGAGCTGTTATTGC+TGG 0.403865 4.3:-55071582 MS.gene073242:CDS
GATTGGAAATGCTTCAATGA+TGG 0.406919 4.3:+55070529 None:intergenic
ACAATTTGAGTTAAGGATAG+TGG 0.413559 4.3:+55071969 None:intergenic
GATGTTGGTGGTGGTATTGG+AGG 0.413798 4.3:-55071522 MS.gene073242:CDS
AAAGGACAAGCATGCACAAC+TGG 0.416268 4.3:-55071609 MS.gene073242:CDS
TGGTTATGACCAATTTGAAA+TGG 0.426665 4.3:-55071667 MS.gene073242:CDS
GATAATATTGGATCATGTAT+TGG 0.435063 4.3:-55070717 MS.gene073242:CDS
ATGAGTGAAATTGTAAAAGA+AGG 0.441259 4.3:-55071723 MS.gene073242:CDS
AAGGCAATACCAGAGAAAAC+TGG 0.443626 4.3:-55070746 MS.gene073242:CDS
TTTCACTCATAAAGTTAAAA+GGG 0.448814 4.3:+55071736 None:intergenic
ACTGCTTTCTTTAGGTGTCA+TGG 0.450664 4.3:-55071687 MS.gene073242:CDS
AGGAAAGGACTGAAGAGAAC+TGG 0.459870 4.3:-55070616 MS.gene073242:CDS
GTTATTGCTGGATACAAAGA+TGG 0.468883 4.3:-55071570 MS.gene073242:CDS
CATTACTCTTAAAAGTAAAG+TGG 0.485083 4.3:+55071914 None:intergenic
AAAAGTGACATTGGCACCAT+TGG 0.486750 4.3:-55071790 MS.gene073242:CDS
TTAAAAGTAAAGTGGAATCA+GGG 0.488590 4.3:+55071922 None:intergenic
AAACATGTCTCCTCCAACAT+GGG 0.491810 4.3:+55071401 None:intergenic
TGCACACAACTCTGGTGGTA+AGG 0.502617 4.3:-55070636 MS.gene073242:CDS
AAATACTTATTCAAAGAGAC+AGG 0.505650 4.3:-55070593 MS.gene073242:CDS
GGTAGTGGATTCTTCATGAC+TGG 0.515619 4.3:-55070808 MS.gene073242:intron
ATTTAACCAAATTCAAACAT+TGG 0.521052 4.3:-55071547 MS.gene073242:CDS
TTCAATGAGTTGTTTAACAA+AGG 0.521127 4.3:-55071627 MS.gene073242:CDS
CAAACATTGGTTGATGTTGG+TGG 0.526942 4.3:-55071534 MS.gene073242:CDS
AATTAATACCTTTAATGTGA+GGG 0.528086 4.3:+55071472 None:intergenic
ATTCAAACATTGGTTGATGT+TGG 0.530112 4.3:-55071537 MS.gene073242:CDS
CAAACATGTCTCCTCCAACA+TGG 0.530132 4.3:+55071400 None:intergenic
GATATTGATGAATTGTAGGA+AGG 0.544209 4.3:-55070765 MS.gene073242:CDS
TCAACGGCACCTAGGTATGA+TGG 0.551980 4.3:-55071429 MS.gene073242:CDS
TTTAAGAGTAATGAGAGTCA+TGG 0.559249 4.3:-55071904 MS.gene073242:CDS
TTTGCCTCATGTTGTTTCAA+CGG 0.559425 4.3:-55071445 MS.gene073242:CDS
GATGGCATCACCCATGTTGG+AGG 0.561480 4.3:-55071411 MS.gene073242:CDS
TCATCAATATCTTTATGCAA+TGG 0.562383 4.3:+55070777 None:intergenic
AAATTAATACCTTTAATGTG+AGG 0.563251 4.3:+55071471 None:intergenic
ATATTGTTTGCACACAACTC+TGG 0.563913 4.3:-55070644 MS.gene073242:CDS
TATGATGGCATCACCCATGT+TGG 0.578808 4.3:-55071414 MS.gene073242:CDS
AAGTAAAGTGGAATCAGGGG+AGG 0.581812 4.3:+55071926 None:intergenic
ACAAGACACAAAAGTGACAT+TGG 0.586673 4.3:-55071799 MS.gene073242:CDS
AGGATAGTGGTTTACACTGA+CGG 0.591646 4.3:+55071982 None:intergenic
CCAATTTGAAATGGCTAGTG+TGG 0.597115 4.3:-55071658 MS.gene073242:CDS
ACATTGGTTGATGTTGGTGG+TGG 0.602342 4.3:-55071531 MS.gene073242:CDS
ACAACTCTGGTGGTAAGGAA+AGG 0.616165 4.3:-55070631 MS.gene073242:CDS
TTGTTTGCACACAACTCTGG+TGG 0.628395 4.3:-55070641 MS.gene073242:CDS
TAAAAGTAAAGTGGAATCAG+GGG 0.636147 4.3:+55071923 None:intergenic
GAAACCGGCGAAATTCTCTA+CGG 0.639770 4.3:-55071849 MS.gene073242:CDS
TGAGTCTCTAGCAATACCAA+TGG 0.641421 4.3:+55071774 None:intergenic
TGGGTGATGCCATCATACCT+AGG 0.642333 4.3:+55071420 None:intergenic
CAAGCCGTAGAGAATTTCGC+CGG 0.643000 4.3:+55071845 None:intergenic
ATGTTGTTTCAACGGCACCT+AGG 0.646521 4.3:-55071437 MS.gene073242:CDS
GATCATGTATTGGATCCAGA+AGG 0.647337 4.3:-55070707 MS.gene073242:CDS
AGTAAAGTGGAATCAGGGGA+GGG 0.649036 4.3:+55071927 None:intergenic
AATACCAGAGAAAACTGGAA+AGG 0.659592 4.3:-55070741 MS.gene073242:CDS
GGTGCCGTTGAAACAACATG+AGG 0.729016 4.3:+55071441 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTCTAACTATAAATATAAAA+TGG - chr4.3:55071685-55071704 MS.gene073242:CDS 15.0%
!! TACTAAATATACGTTATAAA+TGG - chr4.3:55071410-55071429 MS.gene073242:CDS 15.0%
!! TAGAATTAGTTGAATATTAA+CGG - chr4.3:55071663-55071682 MS.gene073242:CDS 15.0%
!!! AAATACGTCTTTTAAATTTT+AGG - chr4.3:55071613-55071632 MS.gene073242:CDS 15.0%
!! AAATTAATACCTTTAATGTG+AGG + chr4.3:55071108-55071127 None:intergenic 20.0%
!! AATTAATACCTTTAATGTGA+GGG + chr4.3:55071107-55071126 None:intergenic 20.0%
!! ATTTAACCAAATTCAAACAT+TGG - chr4.3:55071029-55071048 MS.gene073242:intron 20.0%
!! ATTTCACTCATAAAGTTAAA+AGG + chr4.3:55070844-55070863 None:intergenic 20.0%
!! TAAAGATATTGATGAATTGT+AGG - chr4.3:55071807-55071826 MS.gene073242:CDS 20.0%
!! TTTCACTCATAAAGTTAAAA+GGG + chr4.3:55070843-55070862 None:intergenic 20.0%
!!! CGATAAATTTTGTCTATTTT+GGG - chr4.3:55071487-55071506 MS.gene073242:CDS 20.0%
!!! TAAACAACTCATTGAATTTT+GGG + chr4.3:55070945-55070964 None:intergenic 20.0%
!!! TCGATAAATTTTGTCTATTT+TGG - chr4.3:55071486-55071505 MS.gene073242:CDS 20.0%
!!! TTAAACAACTCATTGAATTT+TGG + chr4.3:55070946-55070965 None:intergenic 20.0%
! AAATACTTATTCAAAGAGAC+AGG - chr4.3:55071983-55072002 MS.gene073242:CDS 25.0%
! AATGAATATCTTTGTGTGAA+TGG - chr4.3:55071746-55071765 MS.gene073242:CDS 25.0%
! ATGAATATCTTTGTGTGAAT+GGG - chr4.3:55071747-55071766 MS.gene073242:CDS 25.0%
! ATGAGTGAAATTGTAAAAGA+AGG - chr4.3:55070853-55070872 MS.gene073242:intron 25.0%
! CATTACTCTTAAAAGTAAAG+TGG + chr4.3:55070665-55070684 None:intergenic 25.0%
! GATAATATTGGATCATGTAT+TGG - chr4.3:55071859-55071878 MS.gene073242:CDS 25.0%
! TAAAAGGGTTCATATGAATT+GGG + chr4.3:55070828-55070847 None:intergenic 25.0%
! TCATCAATATCTTTATGCAA+TGG + chr4.3:55071802-55071821 None:intergenic 25.0%
! TTAAAAGGGTTCATATGAAT+TGG + chr4.3:55070829-55070848 None:intergenic 25.0%
! TTAAAAGTAAAGTGGAATCA+GGG + chr4.3:55070657-55070676 None:intergenic 25.0%
! TTCAATGAGTTGTTTAACAA+AGG - chr4.3:55070949-55070968 MS.gene073242:intron 25.0%
!! AAATCGAAAGATTTTACTCA+AGG - chr4.3:55071315-55071334 MS.gene073242:intron 25.0%
!! TTTTAATGATGTTGTAACGA+GGG + chr4.3:55072012-55072031 None:intergenic 25.0%
!!! ATTTTAATGATGTTGTAACG+AGG + chr4.3:55072013-55072032 None:intergenic 25.0%
AAAATCGCAATCAAAATCGA+TGG - chr4.3:55071367-55071386 MS.gene073242:CDS 30.0%
AACTGGAAAGGTGATAATAT+TGG - chr4.3:55071847-55071866 MS.gene073242:CDS 30.0%
ATGATCGTATATACTAACCA+AGG - chr4.3:55071259-55071278 MS.gene073242:intron 30.0%
CATCAACCAATGTTTGAATT+TGG + chr4.3:55071038-55071057 None:intergenic 30.0%
CTAACCAAGGTTATCAAAAT+CGG - chr4.3:55071272-55071291 MS.gene073242:intron 30.0%
CTTAAAAGTAAAGTGGAATC+AGG + chr4.3:55070658-55070677 None:intergenic 30.0%
GATATTGATGAATTGTAGGA+AGG - chr4.3:55071811-55071830 MS.gene073242:CDS 30.0%
GATGAACCATTTACTGATAT+TGG - chr4.3:55071893-55071912 MS.gene073242:CDS 30.0%
TAAAAGTAAAGTGGAATCAG+GGG + chr4.3:55070656-55070675 None:intergenic 30.0%
TAACCAAGGTTATCAAAATC+GGG - chr4.3:55071273-55071292 MS.gene073242:intron 30.0%
TGGTTATGACCAATTTGAAA+TGG - chr4.3:55070909-55070928 MS.gene073242:intron 30.0%
TTTAAGAGTAATGAGAGTCA+TGG - chr4.3:55070672-55070691 MS.gene073242:CDS 30.0%
! ACAATTTGAGTTAAGGATAG+TGG + chr4.3:55070610-55070629 None:intergenic 30.0%
! ATTCAAACATTGGTTGATGT+TGG - chr4.3:55071039-55071058 MS.gene073242:intron 30.0%
! ATTCTGCACAATTTGAGTTA+AGG + chr4.3:55070617-55070636 None:intergenic 30.0%
! GACTTTTGTAGAATCGATAT+AGG - chr4.3:55071337-55071356 MS.gene073242:intron 30.0%
! GTAAAAGAAGGCACAAAAAA+TGG - chr4.3:55070865-55070884 MS.gene073242:intron 30.0%
!!! CTGGATTTTAGGATTTAAGT+CGG - chr4.3:55071558-55071577 MS.gene073242:CDS 30.0%
!!! TCTATTTTGGGATTCTACTA+TGG - chr4.3:55071499-55071518 MS.gene073242:CDS 30.0%
!!! TGGATTTTAGGATTTAAGTC+GGG - chr4.3:55071559-55071578 MS.gene073242:CDS 30.0%
AAAATGGCACTGCTTTCTTT+AGG - chr4.3:55070881-55070900 MS.gene073242:intron 35.0%
AATACCAGAGAAAACTGGAA+AGG - chr4.3:55071835-55071854 MS.gene073242:CDS 35.0%
ACAAGACACAAAAGTGACAT+TGG - chr4.3:55070777-55070796 MS.gene073242:CDS 35.0%
AGTGCAGAAAAATCAGAAAC+CGG - chr4.3:55070712-55070731 MS.gene073242:CDS 35.0%
ATATTGTTTGCACACAACTC+TGG - chr4.3:55071932-55071951 MS.gene073242:CDS 35.0%
CGACTTAAATCCTAAAATCC+AGG + chr4.3:55071560-55071579 None:intergenic 35.0%
CTATGGATTTAAGTCGTTGA+TGG - chr4.3:55071516-55071535 MS.gene073242:CDS 35.0%
GATTGGAAATGCTTCAATGA+TGG + chr4.3:55072050-55072069 None:intergenic 35.0%
GCTATGCCAATATCAGTAAA+TGG + chr4.3:55071902-55071921 None:intergenic 35.0%
GTTATTGCTGGATACAAAGA+TGG - chr4.3:55071006-55071025 MS.gene073242:intron 35.0%
!! TTTGCCTCATGTTGTTTCAA+CGG - chr4.3:55071131-55071150 MS.gene073242:intron 35.0%
!!! TTTTGCTCTAAAAGCAGTTG+TGG - chr4.3:55070546-55070565 MS.gene073242:CDS 35.0%
AAAAGTGACATTGGCACCAT+TGG - chr4.3:55070786-55070805 MS.gene073242:CDS 40.0%
AAACATGTCTCCTCCAACAT+GGG + chr4.3:55071178-55071197 None:intergenic 40.0%
AAGGCAATACCAGAGAAAAC+TGG - chr4.3:55071830-55071849 MS.gene073242:CDS 40.0%
ACTGCTTTCTTTAGGTGTCA+TGG - chr4.3:55070889-55070908 MS.gene073242:intron 40.0%
AGAGCTTACCCTCACATTAA+AGG - chr4.3:55071096-55071115 MS.gene073242:intron 40.0%
ATCTTTGTGTGAATGGGTAG+TGG - chr4.3:55071753-55071772 MS.gene073242:CDS 40.0%
CCACACTAGCCATTTCAAAT+TGG + chr4.3:55070921-55070940 None:intergenic 40.0%
GATCATGTATTGGATCCAGA+AGG - chr4.3:55071869-55071888 MS.gene073242:CDS 40.0%
GTAAATGGTTCATCTCCTTC+TGG + chr4.3:55071887-55071906 None:intergenic 40.0%
TGAAGAAAGTCGCAAAATCC+TGG - chr4.3:55071539-55071558 MS.gene073242:CDS 40.0%
TGAGTCTCTAGCAATACCAA+TGG + chr4.3:55070805-55070824 None:intergenic 40.0%
! AGGATAGTGGTTTACACTGA+CGG + chr4.3:55070597-55070616 None:intergenic 40.0%
! ATCACCTTTCCAGTTTTCTC+TGG + chr4.3:55071842-55071861 None:intergenic 40.0%
! CAAACATTGGTTGATGTTGG+TGG - chr4.3:55071042-55071061 MS.gene073242:intron 40.0%
! CCAATTTGAAATGGCTAGTG+TGG - chr4.3:55070918-55070937 MS.gene073242:intron 40.0%
! GTCGCAAAATCCTGGATTTT+AGG - chr4.3:55071547-55071566 MS.gene073242:CDS 40.0%
! GTGTTTAGAGCTGTTATTGC+TGG - chr4.3:55070994-55071013 MS.gene073242:intron 40.0%
!! AGTCCCGATTTTGATAACCT+TGG + chr4.3:55071279-55071298 None:intergenic 40.0%
AAAGGACAAGCATGCACAAC+TGG - chr4.3:55070967-55070986 MS.gene073242:intron 45.0%
AAGTAAAGTGGAATCAGGGG+AGG + chr4.3:55070653-55070672 None:intergenic 45.0%
ACAACTCTGGTGGTAAGGAA+AGG - chr4.3:55071945-55071964 MS.gene073242:CDS 45.0%
AGGAAAGGACTGAAGAGAAC+TGG - chr4.3:55071960-55071979 MS.gene073242:CDS 45.0%
AGTAAAGTGGAATCAGGGGA+GGG + chr4.3:55070652-55070671 None:intergenic 45.0%
CAAACATGTCTCCTCCAACA+TGG + chr4.3:55071179-55071198 None:intergenic 45.0%
GAAACCGGCGAAATTCTCTA+CGG - chr4.3:55070727-55070746 MS.gene073242:CDS 45.0%
GCTTGACACGTGCATCAAAA+TGG - chr4.3:55070749-55070768 MS.gene073242:CDS 45.0%
TATGATGGCATCACCCATGT+TGG - chr4.3:55071162-55071181 MS.gene073242:intron 45.0%
TTGTTTGCACACAACTCTGG+TGG - chr4.3:55071935-55071954 MS.gene073242:CDS 45.0%
! ACATTGGTTGATGTTGGTGG+TGG - chr4.3:55071045-55071064 MS.gene073242:intron 45.0%
!! ATGTTGTTTCAACGGCACCT+AGG - chr4.3:55071139-55071158 MS.gene073242:intron 45.0%
!! GGTAGTGGATTCTTCATGAC+TGG - chr4.3:55071768-55071787 MS.gene073242:CDS 45.0%
!! GTTGATGTTGGTGGTGGTAT+TGG - chr4.3:55071051-55071070 MS.gene073242:intron 45.0%
!!! ATATTTAGTAATAATTTTTT+TGG + chr4.3:55071400-55071419 None:intergenic 5.0%
!!! TATTTAGTAATAATTTTTTT+GGG + chr4.3:55071399-55071418 None:intergenic 5.0%
CAAGCCGTAGAGAATTTCGC+CGG + chr4.3:55070734-55070753 None:intergenic 50.0%
TCAACGGCACCTAGGTATGA+TGG - chr4.3:55071147-55071166 MS.gene073242:intron 50.0%
TGCACACAACTCTGGTGGTA+AGG - chr4.3:55071940-55071959 MS.gene073242:CDS 50.0%
TGGGTGATGCCATCATACCT+AGG + chr4.3:55071159-55071178 None:intergenic 50.0%
!! GATGTTGGTGGTGGTATTGG+AGG - chr4.3:55071054-55071073 MS.gene073242:intron 50.0%
!! GGTGCCGTTGAAACAACATG+AGG + chr4.3:55071138-55071157 None:intergenic 50.0%
GATGGCATCACCCATGTTGG+AGG - chr4.3:55071165-55071184 MS.gene073242:intron 55.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 55070526 55072072 55070526 ID=MS.gene073242
chr4.3 mRNA 55070526 55072072 55070526 ID=MS.gene073242.t1;Parent=MS.gene073242
chr4.3 exon 55071365 55072072 55071365 ID=MS.gene073242.t1.exon1;Parent=MS.gene073242.t1
chr4.3 CDS 55071365 55072072 55071365 ID=cds.MS.gene073242.t1;Parent=MS.gene073242.t1
chr4.3 exon 55070526 55070825 55070526 ID=MS.gene073242.t1.exon2;Parent=MS.gene073242.t1
chr4.3 CDS 55070526 55070825 55070526 ID=cds.MS.gene073242.t1;Parent=MS.gene073242.t1
Gene Sequence

>MS.gene073242

ATGACACAATTTACTGACTCTTTTGCTCTAAAAGCAGTTGTGGAGCTACGTATAGCTGATATTATAGACCGTCAGTGTAAACCACTATCCTTAACTCAAATTGTGCAGAATCTTGAAGATGCACCCTCCCCTGATTCCACTTTACTTTTAAGAGTAATGAGAGTCATGGTTCGTAGAAAGATTTTCAGTGCAGAAAAATCAGAAACCGGCGAAATTCTCTACGGCTTGACACGTGCATCAAAATGGATTCTACAAGACACAAAAGTGACATTGGCACCATTGGTATTGCTAGAGACTCACCCAATTCATATGAACCCTTTTAACTTTATGAGTGAAATTGTAAAAGAAGGCACAAAAAATGGCACTGCTTTCTTTAGGTGTCATGGTTATGACCAATTTGAAATGGCTAGTGTGGACCCAAAATTCAATGAGTTGTTTAACAAAGGACAAGCATGCACAACTGGATTTGTGTTTAGAGCTGTTATTGCTGGATACAAAGATGGATTTAACCAAATTCAAACATTGGTTGATGTTGGTGGTGGTATTGGAGGATCTATGTCTGAGATTGTTAGAGCTTACCCTCACATTAAAGGTATTAATTTTGATTTGCCTCATGTTGTTTCAACGGCACCTAGGTATGATGGCATCACCCATGTTGGAGGAGACATGTTTGTTTCTATTTCTAGTGCTGATGCTATTTACATCAAAGTATAACTCTAAACATATGAATTTTATGATCGTATATACTAACCAAGGTTATCAAAATCGGGACTTGTAAAATCGCATTCAAAATCGAAAGATTTTACTCAAGGACTTTTGTAGAATCGATATAGGAAACTGCAAAATCGCAATCAAAATCGATGGATTTTACCCAAAAAAATTATTACTAAATATACGTTATAAATGGTATATAGTCATAAATGACTTAGTTTATAAGAAATCGTAAAATCGCAATCAAAATCGATAAATTTTGTCTATTTTGGGATTCTACTATGGATTTAAGTCGTTGATGGTGAAGAAAGTCGCAAAATCCTGGATTTTAGGATTTAAGTCGGGATTTTGACAACAATGATACTAACACTCTTTCAAATACGTCTTTTAAATTTTAGGTATTGTTATAGATAGTTTGAGATGTGTTAGAATTAGTTGAATATTAACGGTCTAACTATAAATATAAAATGGATAATAACATGTTTATTTGAGAATTGCATATATAATATAATGAATATCTTTGTGTGAATGGGTAGTGGATTCTTCATGACTGGAGTGATGACCATTGCATAAAGATATTGATGAATTGTAGGAAGGCAATACCAGAGAAAACTGGAAAGGTGATAATATTGGATCATGTATTGGATCCAGAAGGAGATGAACCATTTACTGATATTGGCATAGCATTTGACATGATATTGTTTGCACACAACTCTGGTGGTAAGGAAAGGACTGAAGAGAACTGGAAATACTTATTCAAAGAGACAGGATTCCCTCGTTACAACATCATTAAAATCAATGCTTTGCTATCCATCATTGAAGCATTTCCAATCTAA
Protein sequence

>MS.gene073242.t1

MTQFTDSFALKAVVELRIADIIDRQCKPLSLTQIVQNLEDAPSPDSTLLLRVMRVMVRRKIFSAEKSETGEILYGLTRASKWILQDTKVTLAPLVLLETHPIHMNPFNFMSEIVKEGTKNGTAFFRCHGYDQFEMASVDPKFNELFNKGQACTTGFVFRAVIAGYKDGFNQIQTLVDVGGGIGGSMSEIVRAYPHIKGINFDLPHVVSTAPRYDGITHVGGDMFVSISSADAIYIKWILHDWSDDHCIKILMNCRKAIPEKTGKVIILDHVLDPEGDEPFTDIGIAFDMILFAHNSGGKERTEENWKYLFKETGFPRYNIIKINALLSIIEAFPI