AlfalfaGEDB | MS.gene073260

M. sativa cultivar XinJiangDaYe / MS.gene073260

KEGG: K14492
GO: GO:0000160 GO:0007165 GO:0008150 GO:0009987 GO:0035556 GO:0050789 GO:0050794 GO:0065007
PFAM: PF00072.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073260.t1	XP_004511028.2	85.6	160	15	1	1	152	45	204	2.80E-67	264.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073260.t1	O80366	67.7	155	40	1	1	145	1	155	1.3e-52	207.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073260.t1	A0A1S2YWH2	85.6	160	15	1	1	152	45	204	2.0e-67	264.6

TFs/TRs:

Gene ID	Type	Classification
MS.gene073260.t1	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene054801	MS.gene073260	0.800751	1.24E-48	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073260.t1	MTR_8g038620	85.806	155	14	1	1	147	1	155	9.26e-94	269
MS.gene073260.t1	MTR_6g007460	68.780	205	11	2	1	152	3	207	4.44e-93	268
MS.gene073260.t1	MTR_7g490310	69.427	157	39	1	1	148	1	157	8.57e-79	233
MS.gene073260.t1	MTR_3g015490	70.968	155	33	2	7	152	2	153	1.67e-73	217
MS.gene073260.t1	MTR_3g088630	54.717	159	53	3	9	149	22	179	1.22e-55	174
MS.gene073260.t1	MTR_4g106590	57.639	144	60	1	4	146	21	164	1.04e-51	164
MS.gene073260.t1	MTR_3g093860	60.305	131	48	2	10	137	19	148	2.38e-51	161
MS.gene073260.t1	MTR_5g036480	55.479	146	59	2	8	148	15	159	4.36e-51	163
MS.gene073260.t1	MTR_3g078613	51.678	149	72	0	4	152	18	166	5.13e-51	161
MS.gene073260.t1	MTR_1g049100	59.559	136	53	2	8	141	7	142	1.41e-49	158
MS.gene073260.t1	MTR_3g078613	51.333	150	72	1	4	152	18	167	3.75e-49	157
MS.gene073260.t1	MTR_4g051330	74.242	66	17	0	87	152	77	142	6.66e-31	108
MS.gene073260.t1	MTR_4g061360	29.545	132	84	2	7	138	87	209	4.60e-15	71.6
MS.gene073260.t1	MTR_8g024260	29.008	131	84	1	11	141	38	159	4.68e-15	71.6
MS.gene073260.t1	MTR_8g079940	30.769	143	87	3	4	146	24	154	8.82e-15	70.9
MS.gene073260.t1	MTR_1g067110	28.125	128	83	2	11	138	87	205	3.26e-13	66.2
MS.gene073260.t1	MTR_1g067110	28.125	128	83	2	11	138	87	205	3.30e-13	66.2
MS.gene073260.t1	MTR_7g117705	31.429	140	83	4	11	150	11	137	5.37e-13	65.5
MS.gene073260.t1	MTR_1g013180	30.000	130	79	2	11	140	162	279	1.25e-12	64.7
MS.gene073260.t1	MTR_1g013170	29.286	140	85	3	11	150	11	136	1.51e-12	64.3
MS.gene073260.t1	MTR_3g086100	28.676	136	85	2	11	146	19	142	1.55e-12	64.3
MS.gene073260.t1	MTR_1g032570	30.216	139	85	3	9	147	15	141	1.84e-12	63.9
MS.gene073260.t1	MTR_7g118260	26.515	132	88	1	7	138	12	134	1.83e-11	61.2
MS.gene073260.t1	MTR_7g118260	26.515	132	88	1	7	138	12	134	2.04e-11	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073260.t1	AT3G57040	67.742	155	40	1	1	145	1	155	1.18e-73	220
MS.gene073260.t1	AT3G57040	67.742	155	40	1	1	145	1	155	1.18e-73	220
MS.gene073260.t1	AT2G41310	63.871	155	48	1	1	147	1	155	9.67e-71	213
MS.gene073260.t1	AT1G59940	61.832	131	50	0	8	138	32	162	1.40e-55	174
MS.gene073260.t1	AT1G59940	61.832	131	50	0	8	138	124	254	4.91e-54	173
MS.gene073260.t1	AT3G48100	58.273	139	57	1	2	139	18	156	6.10e-54	168
MS.gene073260.t1	AT1G10470	60.305	131	52	0	8	138	19	149	7.33e-54	170
MS.gene073260.t1	AT1G10470	60.305	131	52	0	8	138	33	163	9.20e-54	171
MS.gene073260.t1	AT5G62920	61.538	130	50	0	9	138	25	154	2.09e-52	164
MS.gene073260.t1	AT1G19050	55.147	136	61	0	8	143	23	158	5.42e-52	164
MS.gene073260.t1	AT1G74890	56.934	137	59	0	8	144	17	153	9.55e-52	164
MS.gene073260.t1	AT3G56380	53.623	138	63	1	4	140	15	152	4.77e-51	160
MS.gene073260.t1	AT3G56380	53.237	139	63	2	4	140	15	153	3.06e-49	155
MS.gene073260.t1	AT2G40670	52.518	139	57	2	8	140	28	163	4.69e-49	155
MS.gene073260.t1	AT2G40670	52.143	140	57	3	8	140	28	164	3.31e-47	150
MS.gene073260.t1	AT2G07440	65.574	61	21	0	87	147	9	69	8.05e-23	87.8
MS.gene073260.t1	AT1G10470	58.491	53	22	0	86	138	35	87	3.29e-16	72.0
MS.gene073260.t1	AT2G25180	32.308	130	76	3	11	140	19	136	8.58e-14	67.8
MS.gene073260.t1	AT5G07210	28.472	144	90	3	8	151	15	145	1.90e-13	67.0
MS.gene073260.t1	AT5G60100	30.469	128	80	2	11	138	66	184	6.39e-13	65.5
MS.gene073260.t1	AT5G60100	30.469	128	80	2	11	138	66	184	6.39e-13	65.5
MS.gene073260.t1	AT5G60100	30.469	128	80	2	11	138	66	184	6.39e-13	65.5
MS.gene073260.t1	AT5G60100	30.469	128	80	2	11	138	66	184	7.89e-13	65.1
MS.gene073260.t1	AT5G02810	27.344	128	84	2	11	138	80	198	9.20e-13	65.1
MS.gene073260.t1	AT1G67710	28.148	135	85	2	9	143	11	133	1.07e-12	64.7
MS.gene073260.t1	AT5G60100	30.469	128	80	2	11	138	66	184	1.46e-12	64.3
MS.gene073260.t1	AT5G60100	30.469	128	80	2	11	138	66	184	1.46e-12	64.3
MS.gene073260.t1	AT5G60100	30.469	128	80	2	11	138	66	184	1.46e-12	64.3
MS.gene073260.t1	AT3G16857	28.889	135	84	3	9	143	37	159	1.00e-11	62.0
MS.gene073260.t1	AT3G16857	28.889	135	84	3	9	143	37	159	1.01e-11	62.0
MS.gene073260.t1	AT2G46790	25.170	147	100	3	6	152	34	170	1.93e-11	61.2
MS.gene073260.t1	AT2G46790	25.170	147	100	3	6	152	34	170	1.96e-11	61.2
MS.gene073260.t1	AT1G49190	28.788	132	82	3	10	141	35	154	3.19e-11	60.5
MS.gene073260.t1	AT5G24470	27.820	133	85	2	9	140	50	172	5.27e-11	60.1

Find 25 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGGTATGTCTTTAAAAGATT+TGG	0.160260	4.3:-55505089	None:intergenic
CTGATTGTTGAACTGGTTTC+AGG	0.173333	4.3:-55505324	None:intergenic
AGAGACTCAGTTCCATGTTT+TGG	0.251951	4.3:+55504203	MS.gene073260:CDS
AGTAAGGCTTTAAAATTTCT+TGG	0.266953	4.3:+55504496	MS.gene073260:CDS
TCTCACCTGCTAATCCTTGA+TGG	0.304860	4.3:-55505139	None:intergenic
GGCTTTAAAATTTCTTGGTT+TGG	0.308229	4.3:+55504501	MS.gene073260:CDS
TTTACATCTGATTGTTGAAC+TGG	0.343425	4.3:-55505331	None:intergenic
TCTTGGTTTGGTTGAGGATG+TGG	0.414547	4.3:+55504513	MS.gene073260:CDS
TTTGATGAAATCAAGAGTTA+AGG	0.416978	4.3:+55505369	MS.gene073260:CDS
TACAATGGACTCTGCTAGTA+AGG	0.427203	4.3:+55504480	MS.gene073260:CDS
TATTGTATGCCAGGGATGAC+TGG	0.449232	4.3:+55504679	MS.gene073260:CDS
AAAATTTCTTGGTTTGGTTG+AGG	0.449963	4.3:+55504507	MS.gene073260:CDS
ACTCTTGATTTCATCAAATG+AGG	0.455768	4.3:-55505364	None:intergenic
ATAACAGATTATTGTATGCC+AGG	0.463952	4.3:+55504670	MS.gene073260:CDS
ATGTTCCATCAAGGATTAGC+AGG	0.504757	4.3:+55505134	MS.gene073260:CDS
TTGATAGAATGTTGATTGAG+AGG	0.516155	4.3:+55504244	MS.gene073260:CDS
GATGTGGCTTCAGAAGTTCA+TGG	0.523538	4.3:+55504529	MS.gene073260:CDS
ATGTGGCTTCAGAAGTTCAT+GGG	0.534908	4.3:+55504530	MS.gene073260:CDS
CTTCAGAGAATGTTCCATCA+AGG	0.539392	4.3:+55505125	MS.gene073260:CDS
AGATCATAGCCAGTCATCCC+TGG	0.546401	4.3:-55504688	None:intergenic
TCTGAAGACATAATCACAAT+CGG	0.548679	4.3:-55505109	None:intergenic
TAACAGATTATTGTATGCCA+GGG	0.564090	4.3:+55504671	MS.gene073260:CDS
TATCATCAACAGCCAAAACA+TGG	0.580138	4.3:-55504215	None:intergenic
TGAAATGATAGTTACTACAA+TGG	0.624009	4.3:+55504465	MS.gene073260:intron
TGTGGCTTCAGAAGTTCATG+GGG	0.695893	4.3:+55504531	MS.gene073260:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATTTTTCTTAATATTATGA+TGG	+	chr4.3:55504568-55504587	MS.gene073260:intron	10.0%
!!!	ATTATTTTGTTTTTCTATTT+AGG	+	chr4.3:55504868-55504887	MS.gene073260:intron	10.0%
!!!	GATAATTATTATTTATTTTG+TGG	+	chr4.3:55504434-55504453	MS.gene073260:intron	10.0%
!!!	TTTGTTTTCTTTATAATTTT+GGG	-	chr4.3:55505009-55505028	None:intergenic	10.0%
!!!	CTTTGTTTTCTTTATAATTT+TGG	-	chr4.3:55505010-55505029	None:intergenic	15.0%
!!	TTTCTTAAATAGAAGTGATT+TGG	+	chr4.3:55504916-55504935	MS.gene073260:intron	20.0%
!!!	ATATATGAACATAGTTTGTA+TGG	+	chr4.3:55504357-55504376	MS.gene073260:intron	20.0%
!!!	TAACATCAAGTTAACAATTT+TGG	-	chr4.3:55505039-55505058	None:intergenic	20.0%
!!!	TCAATATACTATTTTGTTGA+AGG	+	chr4.3:55504380-55504399	MS.gene073260:intron	20.0%
!	ATTGTAATGATGTAATGTGA+TGG	+	chr4.3:55504328-55504347	MS.gene073260:intron	25.0%
!	TAAACAATTCTTTGATTCTC+AGG	+	chr4.3:55505063-55505082	MS.gene073260:intron	25.0%
!	TGAAAATGAGCACAAAATTA+TGG	-	chr4.3:55504818-55504837	None:intergenic	25.0%
!	TGAAATGATAGTTACTACAA+TGG	+	chr4.3:55504465-55504484	MS.gene073260:intron	25.0%
!	TTGATTCATATTATTCATGC+AGG	+	chr4.3:55504627-55504646	MS.gene073260:intron	25.0%
!	TTGTAATGATGTAATGTGAT+GGG	+	chr4.3:55504329-55504348	MS.gene073260:intron	25.0%
!	TTTGATGAAATCAAGAGTTA+AGG	+	chr4.3:55505369-55505388	MS.gene073260:CDS	25.0%
!!!	AGTAAGGCTTTAAAATTTCT+TGG	+	chr4.3:55504496-55504515	MS.gene073260:CDS	25.0%
!!!	TGATTAGTGATTACTGATTT+GGG	+	chr4.3:55504978-55504997	MS.gene073260:intron	25.0%
	ACTCTTGATTTCATCAAATG+AGG	-	chr4.3:55505367-55505386	None:intergenic	30.0%
	ATAACAGATTATTGTATGCC+AGG	+	chr4.3:55504670-55504689	MS.gene073260:CDS	30.0%
	ATGTGAAGTCTCACATATTA+TGG	+	chr4.3:55505247-55505266	MS.gene073260:intron	30.0%
	TAACAGATTATTGTATGCCA+GGG	+	chr4.3:55504671-55504690	MS.gene073260:CDS	30.0%
	TCTGAAGACATAATCACAAT+CGG	-	chr4.3:55505112-55505131	None:intergenic	30.0%
	TTTACATCTGATTGTTGAAC+TGG	-	chr4.3:55505334-55505353	None:intergenic	30.0%
!	CATGAAAAGAAGAAGTTTTG+AGG	-	chr4.3:55504272-55504291	None:intergenic	30.0%
!	CGGTATGTCTTTAAAAGATT+TGG	-	chr4.3:55505092-55505111	None:intergenic	30.0%
!	CTCAAAACTTCTTCTTTTCA+TGG	+	chr4.3:55504270-55504289	MS.gene073260:CDS	30.0%
!	CTGATTAGTGATTACTGATT+TGG	+	chr4.3:55504977-55504996	MS.gene073260:intron	30.0%
!	TTGATAGAATGTTGATTGAG+AGG	+	chr4.3:55504244-55504263	MS.gene073260:CDS	30.0%
!!	AAAATTTCTTGGTTTGGTTG+AGG	+	chr4.3:55504507-55504526	MS.gene073260:CDS	30.0%
!!	GGCTTTAAAATTTCTTGGTT+TGG	+	chr4.3:55504501-55504520	MS.gene073260:CDS	30.0%
!!!	TGTATCAGATGTTTTGAAGA+GGG	+	chr4.3:55505286-55505305	MS.gene073260:intron	30.0%
!!!	TTGTATCAGATGTTTTGAAG+AGG	+	chr4.3:55505285-55505304	MS.gene073260:intron	30.0%
	TATCATCAACAGCCAAAACA+TGG	-	chr4.3:55504218-55504237	None:intergenic	35.0%
	TGATCTGCTCAGAAAAATCA+AGG	+	chr4.3:55504705-55504724	MS.gene073260:CDS	35.0%
	ATGTGGCTTCAGAAGTTCAT+GGG	+	chr4.3:55504530-55504549	MS.gene073260:CDS	40.0%
	CTGCTCAGAAAAATCAAGGT+AGG	+	chr4.3:55504709-55504728	MS.gene073260:intron	40.0%
	CTTCAGAGAATGTTCCATCA+AGG	+	chr4.3:55505125-55505144	MS.gene073260:CDS	40.0%
!	AGAGACTCAGTTCCATGTTT+TGG	+	chr4.3:55504203-55504222	MS.gene073260:CDS	40.0%
!	ATGTTCCATCAAGGATTAGC+AGG	+	chr4.3:55505134-55505153	MS.gene073260:CDS	40.0%
!	CTGATTGTTGAACTGGTTTC+AGG	-	chr4.3:55505327-55505346	None:intergenic	40.0%
!	TACAATGGACTCTGCTAGTA+AGG	+	chr4.3:55504480-55504499	MS.gene073260:CDS	40.0%
	GATGTGGCTTCAGAAGTTCA+TGG	+	chr4.3:55504529-55504548	MS.gene073260:CDS	45.0%
	TATTGTATGCCAGGGATGAC+TGG	+	chr4.3:55504679-55504698	MS.gene073260:CDS	45.0%
	TCTCACCTGCTAATCCTTGA+TGG	-	chr4.3:55505142-55505161	None:intergenic	45.0%
	TGTGGCTTCAGAAGTTCATG+GGG	+	chr4.3:55504531-55504550	MS.gene073260:CDS	45.0%
!!	TCTTGGTTTGGTTGAGGATG+TGG	+	chr4.3:55504513-55504532	MS.gene073260:CDS	45.0%
	AGATCATAGCCAGTCATCCC+TGG	-	chr4.3:55504691-55504710	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	55504189	55505423	55504189	ID=MS.gene073260
chr4.3	mRNA	55504189	55505423	55504189	ID=MS.gene073260.t1;Parent=MS.gene073260
chr4.3	exon	55504189	55504291	55504189	ID=MS.gene073260.t1.exon1;Parent=MS.gene073260.t1
chr4.3	CDS	55504189	55504291	55504189	ID=cds.MS.gene073260.t1;Parent=MS.gene073260.t1
chr4.3	exon	55504476	55504552	55504476	ID=MS.gene073260.t1.exon2;Parent=MS.gene073260.t1
chr4.3	CDS	55504476	55504552	55504476	ID=cds.MS.gene073260.t1;Parent=MS.gene073260.t1
chr4.3	exon	55504649	55504726	55504649	ID=MS.gene073260.t1.exon3;Parent=MS.gene073260.t1
chr4.3	CDS	55504649	55504726	55504649	ID=cds.MS.gene073260.t1;Parent=MS.gene073260.t1
chr4.3	exon	55505085	55505155	55505085	ID=MS.gene073260.t1.exon4;Parent=MS.gene073260.t1
chr4.3	CDS	55505085	55505155	55505085	ID=cds.MS.gene073260.t1;Parent=MS.gene073260.t1
chr4.3	exon	55505294	55505423	55505294	ID=MS.gene073260.t1.exon5;Parent=MS.gene073260.t1
chr4.3	CDS	55505294	55505423	55505294	ID=cds.MS.gene073260.t1;Parent=MS.gene073260.t1

Gene Sequence

>MS.gene073260

ATGGGTATGGCTGCAGAGACTCAGTTCCATGTTTTGGCTGTTGATGATAGTATTATTGATAGAATGTTGATTGAGAGGCTCCTCAAAACTTCTTCTTTTCATGGTACTTTTCTTGTTTATTTTTTTAATTTTGATTTATATTGTAATGATGTAATGTGATGGGAAAAAATATATGAACATAGTTTGTATGGTCAATATACTATTTTGTTGAAGGTGTGATTTTTTTGTTACTAAGATTGACAAATGATAATTATTATTTATTTTGTGGTTTTGTATTGAAATGATAGTTACTACAATGGACTCTGCTAGTAAGGCTTTAAAATTTCTTGGTTTGGTTGAGGATGTGGCTTCAGAAGTTCATGGGGTAAAATTCTCAAACAATTTTTCTTAATATTATGATGGCTTAGACTATTTTGATGTATAAGTTTGTATTAATATTTGATTCATATTATTCATGCAGGATGTAGATGTAAATCTGATCATAACAGATTATTGTATGCCAGGGATGACTGGCTATGATCTGCTCAGAAAAATCAAGGTAGGTTCTTGAGATATTGCAATACTTTTTTAAATTGATTTTGTCACTTTTTATTTGATTTGTCTATTGTGAATTTCATTAACTTTTTCCATAATTTTGTGCTCATTTTCAAAATTTGAATGTATCAAAAAGTTTTGTGAAATTATTTTGTTTTTCTATTTAGGTAAGAATTTGAAAAATAGTATCATATTTCTTAAATAGAAGTGATTTGGATTTTCGAATCAAAATAATAAAAATCACTTCTGAATCTCTGATTAGTGATTACTGATTTGGGATATTCCCAAAATTATAAAGAAAACAAAGCTCAAACCAAAATTGTTAACTTGATGTTAAAGCTAAACAATTCTTTGATTCTCAGGAATCCAAATCTTTTAAAGACATACCGATTGTGATTATGTCTTCAGAGAATGTTCCATCAAGGATTAGCAGGTGAGAAAATTGTATACATTTGTTGAAATCAAGTATCAAATGCAGTTGTGAGCACATGATATCTTATAACACATTACTAATTGTTTGTAAAATGTGAAGTCTCACATATTATGGACTAATTGTTTGTAATTGTATCAGATGTTTTGAAGAGGGAGCTGAAGAATTCTTCCTGAAACCAGTTCAACAATCAGATGTAAACAAGCTGAAGCCTCATTTGATGAAATCAAGAGTTAAGGAAGAGCAAGAACAAACAACCCATCAACAATAA

Protein sequence

>MS.gene073260.t1

MGMAAETQFHVLAVDDSIIDRMLIERLLKTSSFHVTTMDSASKALKFLGLVEDVASEVHGDVDVNLIITDYCMPGMTGYDLLRKIKESKSFKDIPIVIMSSENVPSRISRCFEEGAEEFFLKPVQQSDVNKLKPHLMKSRVKEEQEQTTHQQ