AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene054801


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054801.t1 MTR_3g099580 96.296 54 2 0 1 54 17 70 3.25e-32 109
MS.gene054801.t1 MTR_3g099570 81.481 54 10 0 1 54 17 70 4.52e-26 93.6
MS.gene054801.t1 MTR_3g099680 76.364 55 12 1 1 54 17 71 3.19e-22 84.3
MS.gene054801.t1 MTR_3g099980 76.364 55 12 1 1 54 17 71 9.84e-22 83.2
MS.gene054801.t1 MTR_3g099540 73.077 52 14 0 1 52 18 69 1.33e-21 80.9
MS.gene054801.t1 MTR_8g007035 60.000 55 21 1 1 54 14 68 1.18e-15 67.0
MS.gene054801.t1 MTR_8g007020 58.182 55 22 1 1 54 14 68 2.84e-14 63.2
MS.gene054801.t1 MTR_1g090190 50.000 48 24 0 7 54 20 67 4.16e-12 57.4
MS.gene054801.t1 MTR_7g086140 56.250 48 20 1 6 52 22 69 3.04e-11 54.7
MS.gene054801.t1 MTR_7g086190 56.250 48 20 1 6 52 22 69 3.04e-11 54.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054801.t1 AT1G72230 67.568 37 12 0 18 54 31 67 9.19e-14 61.6
MS.gene054801.t1 AT1G22480 64.865 37 13 0 18 54 27 63 8.98e-13 58.9
MS.gene054801.t1 AT2G32300 54.902 51 22 1 5 54 21 71 2.73e-12 58.9
MS.gene054801.t1 AT2G32300 54.902 51 22 1 5 54 51 101 3.86e-12 58.5
MS.gene054801.t1 AT3G60270 48.980 49 25 0 6 54 22 70 7.74e-11 54.3

Find 17 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATAAATCAGGTTGGGCAAT+TGG 0.365257 3.3:-79502232 MS.gene054801:CDS
CTATGGACAGTGTTCAACTA+TGG 0.366108 3.3:-79501991 MS.gene054801:intron
CACACCGTTGGAGATAAATC+AGG 0.494660 3.3:-79502244 MS.gene054801:CDS
GGCTAGTGACAAAACATTTG+CGG 0.495560 3.3:-79502191 MS.gene054801:CDS
TTTGCGGTTGGTGACAGTCT+TGG 0.500707 3.3:-79502175 MS.gene054801:intron
CGTTGGAGATAAATCAGGTT+GGG 0.514147 3.3:-79502239 MS.gene054801:CDS
CTTGCAACTGTCCACACCGT+TGG 0.529565 3.3:-79502256 MS.gene054801:CDS
CCAACCTGATTTATCTCCAA+CGG 0.588831 3.3:+79502240 None:intergenic
GTGTGGACAGTTGCAAGAGT+TGG 0.591396 3.3:+79502262 None:intergenic
AGTGACAAAACATTTGCGGT+TGG 0.594495 3.3:-79502187 MS.gene054801:CDS
CTTTAACTTCATCCACTGTG+TGG 0.606822 3.3:+79501962 None:intergenic
TTGGTTCTGATTATAACACA+TGG 0.617869 3.3:-79502213 MS.gene054801:CDS
ACAGTGTTCAACTATGGTGC+TGG 0.627539 3.3:-79501985 MS.gene054801:intron
CCGTTGGAGATAAATCAGGT+TGG 0.633245 3.3:-79502240 MS.gene054801:CDS
TGGTTCTGATTATAACACAT+GGG 0.658992 3.3:-79502212 MS.gene054801:CDS
CTGATTTATCTCCAACGGTG+TGG 0.663800 3.3:+79502245 None:intergenic
CTATGGTGCTGGCCACACAG+TGG 0.772990 3.3:-79501974 MS.gene054801:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATATATATAACTTTACTA+TGG - chr3.3:79502222-79502241 MS.gene054801:CDS 10.0%
!! TATATTAGATTTGTAAATTG+TGG + chr3.3:79502209-79502228 None:intergenic 15.0%
!! TGGTTCTGATTATAACACAT+GGG - chr3.3:79502018-79502037 MS.gene054801:intron 30.0%
!! TTGGTTCTGATTATAACACA+TGG - chr3.3:79502017-79502036 MS.gene054801:intron 30.0%
AGTGACAAAACATTTGCGGT+TGG - chr3.3:79502043-79502062 MS.gene054801:intron 40.0%
CCAACCTGATTTATCTCCAA+CGG + chr3.3:79501993-79502012 None:intergenic 40.0%
CGTTGGAGATAAATCAGGTT+GGG - chr3.3:79501991-79502010 MS.gene054801:intron 40.0%
CTATGGACAGTGTTCAACTA+TGG - chr3.3:79502239-79502258 MS.gene054801:CDS 40.0%
GGCTAGTGACAAAACATTTG+CGG - chr3.3:79502039-79502058 MS.gene054801:intron 40.0%
! CTTTAACTTCATCCACTGTG+TGG + chr3.3:79502271-79502290 None:intergenic 40.0%
!! GATAAATCAGGTTGGGCAAT+TGG - chr3.3:79501998-79502017 MS.gene054801:intron 40.0%
ACAGTGTTCAACTATGGTGC+TGG - chr3.3:79502245-79502264 MS.gene054801:CDS 45.0%
CACACCGTTGGAGATAAATC+AGG - chr3.3:79501986-79502005 MS.gene054801:intron 45.0%
CCGTTGGAGATAAATCAGGT+TGG - chr3.3:79501990-79502009 MS.gene054801:intron 45.0%
CTGATTTATCTCCAACGGTG+TGG + chr3.3:79501988-79502007 None:intergenic 45.0%
GTGTGGACAGTTGCAAGAGT+TGG + chr3.3:79501971-79501990 None:intergenic 50.0%
!! TTTGCGGTTGGTGACAGTCT+TGG - chr3.3:79502055-79502074 MS.gene054801:intron 50.0%
CTTGCAACTGTCCACACCGT+TGG - chr3.3:79501974-79501993 MS.gene054801:CDS 55.0%
!! CTATGGTGCTGGCCACACAG+TGG - chr3.3:79502256-79502275 MS.gene054801:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 79501959 79502293 79501959 ID=MS.gene054801
chr3.3 mRNA 79501959 79502293 79501959 ID=MS.gene054801.t1;Parent=MS.gene054801
chr3.3 exon 79502176 79502293 79502176 ID=MS.gene054801.t1.exon1;Parent=MS.gene054801.t1
chr3.3 CDS 79502176 79502293 79502176 ID=cds.MS.gene054801.t1;Parent=MS.gene054801.t1
chr3.3 exon 79501959 79502003 79501959 ID=MS.gene054801.t1.exon2;Parent=MS.gene054801.t1
chr3.3 CDS 79501959 79502003 79501959 ID=cds.MS.gene054801.t1;Parent=MS.gene054801.t1
Gene Sequence

>MS.gene054801

ATGGCACTGCCAACTCTTGCAACTGTCCACACCGTTGGAGATAAATCAGGTTGGGCAATTGGTTCTGATTATAACACATGGGCTAGTGACAAAACATTTGCGGTTGGTGACAGTCTTGGTAAGTATTATTAATTATTTCTTTGTTAAACACAGCTTAATGAGAAGAAAGCATGTAAAGATTGTAAACTGAAAAATAGTTAATTGAAGTCTTTGTCAATTTCATAATTCATTTAGTTTTATATTGCAACCACAATTTACAAATCTAATATATATAACTTTACTATGGACAGTGTTCAACTATGGTGCTGGCCACACAGTGGATGAAGTTAAAGAAA

Protein sequence

>MS.gene054801.t1

MALPTLATVHTVGDKSGWAIGSDYNTWASDKTFAVGDSLVFNYGAGHTVDEVKE