AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene073453

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073453.t1 MTR_2g035760 95.697 488 21 0 1 488 10 497 0.0 900
MS.gene073453.t1 MTR_6g015785 70.757 489 134 4 1 488 14 494 0.0 650
MS.gene073453.t1 MTR_4g094732 46.467 467 219 10 34 484 29 480 2.62e-139 409
MS.gene073453.t1 MTR_2g035790 52.113 426 184 7 73 484 69 488 3.30e-136 402
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073453.t1 AT4G20050 61.790 458 158 5 29 484 62 504 0.0 565
MS.gene073453.t1 AT4G20050 61.522 460 160 5 29 486 37 481 0.0 565
MS.gene073453.t1 AT4G20050 61.522 460 160 5 29 486 37 481 0.0 565
MS.gene073453.t1 AT4G20050 61.522 460 160 5 29 486 37 481 0.0 565
MS.gene073453.t1 AT4G20050 61.522 460 160 5 29 486 37 481 0.0 565
MS.gene073453.t1 AT4G20050 64.962 391 127 3 98 486 1 383 0.0 516
MS.gene073453.t1 AT4G20050 64.962 391 127 3 98 486 1 383 0.0 516
MS.gene073453.t1 AT4G20040 49.173 423 198 4 73 484 67 483 1.99e-136 402

Find 98 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATTGTAATTGGTGTTTA+TGG 0.186040 2.1:-55262405 MS.gene073453:CDS
AACTCTCAAGGACCTCTTAT+TGG 0.199279 2.1:-55261292 MS.gene073453:CDS
GTTGTTTGTTTCATTGTAAT+TGG 0.263362 2.1:-55262414 MS.gene073453:CDS
TGGTGTACATTGTTACAATA+AGG 0.274737 2.1:-55260977 MS.gene073453:CDS
TACAATAAGGCCACTGGATT+CGG 0.316472 2.1:-55260964 MS.gene073453:CDS
TGCAACGGGGATGAATTTAA+AGG 0.328386 2.1:-55260650 MS.gene073453:CDS
AGGTGTTGAAATTGTTCATT+TGG 0.332781 2.1:-55260728 MS.gene073453:CDS
TTATTGGATTCAAACTTTAG+AGG 0.335089 2.1:-55261276 MS.gene073453:CDS
AGTTTCTTATGTATGTTTCA+TGG 0.352507 2.1:+55261160 None:intergenic
TTGATCAAGTTATTGTTGAT+AGG 0.379420 2.1:-55260678 MS.gene073453:CDS
ATGTTCCCAACGCTGGTTAC+CGG 0.382500 2.1:+55261882 None:intergenic
AGAATCATGTCTTGTTAAAG+AGG 0.407306 2.1:+55262305 None:intergenic
CTCTGCTGCTATAGGAATCA+TGG 0.408545 2.1:-55261025 MS.gene073453:CDS
GTTGATAGGAACGTTGCAAC+GGG 0.411337 2.1:-55260664 MS.gene073453:CDS
GCCACTGGATTCGGCGGTAC+CGG 0.416083 2.1:-55260955 MS.gene073453:CDS
CAGTACTCATTAAGTTCAAC+AGG 0.423624 2.1:-55260532 MS.gene073453:CDS
ACATTTCTGATAGCATGATT+AGG 0.424152 2.1:+55260499 None:intergenic
CAGAAAGTCACTCAAGTTAC+CGG 0.424279 2.1:-55261901 MS.gene073453:CDS
TCAATGATGTAGCCATCATT+TGG 0.425679 2.1:+55261384 None:intergenic
CTAGAAATATGAAGTTGAAC+AGG 0.426194 2.1:+55260850 None:intergenic
GCTATAGGAATCATGGTAAC+TGG 0.427357 2.1:-55261018 MS.gene073453:CDS
ACTTGATCAACTTGATGAAA+AGG 0.437301 2.1:+55260691 None:intergenic
TACATAAGAAACTCTTTCCT+TGG 0.440272 2.1:-55261150 MS.gene073453:CDS
ATTTCTAGTAGTTTCTTCCT+TGG 0.441880 2.1:-55260835 MS.gene073453:CDS
ATTGTAGATAACATGTTCTC+TGG 0.444931 2.1:-55260760 MS.gene073453:CDS
TCCATCAAATATCCTTCGCT+TGG 0.449157 2.1:+55261981 None:intergenic
TGCAAGGGAATGGAACTTCA+TGG 0.449669 2.1:-55260606 MS.gene073453:CDS
ATTACACATTTCACAACCAA+TGG 0.452369 2.1:-55261201 MS.gene073453:CDS
TCCAAGCGAAGGATATTTGA+TGG 0.464488 2.1:-55261982 MS.gene073453:CDS
GTGCTATCTGAATTTCCTGT+AGG 0.469372 2.1:+55262044 None:intergenic
CCTTTCGTGTTTATCGCCTC+CGG 0.470986 2.1:+55261113 None:intergenic
GATGTCACATGGTATACTCT+TGG 0.471851 2.1:+55262080 None:intergenic
CCTACTAGGAAAATTTCTGA+TGG 0.476121 2.1:-55262369 MS.gene073453:CDS
ATATCCTTCGCTTGGACCGT+TGG 0.476727 2.1:+55261989 None:intergenic
ACCTCATCAAATGAAAATGA+TGG 0.476960 2.1:-55261354 MS.gene073453:CDS
AGTTATGTACTCAAAATTGT+AGG 0.480837 2.1:+55261311 None:intergenic
CGCCTCCGGCGGTTATGTGT+TGG 0.483145 2.1:+55261127 None:intergenic
TAAAGGCTACTGTCGCGAAA+AGG 0.486640 2.1:-55260633 MS.gene073453:CDS
TAAAGTTTGAATCCAATAAG+AGG 0.490425 2.1:+55261280 None:intergenic
TGCTATCTGAATTTCCTGTA+GGG 0.496538 2.1:+55262045 None:intergenic
ACCTCAGCTGGCTGAGAAGA+AGG 0.497308 2.1:+55262246 None:intergenic
TGTATGTTTCATGGCCACTT+TGG 0.501309 2.1:+55261169 None:intergenic
AAAAGGTCGTTGCAAGGGAA+TGG 0.502322 2.1:-55260616 MS.gene073453:CDS
ATTGTATTATATACCTCAGC+TGG 0.511049 2.1:+55262234 None:intergenic
TCCTGTAGGGTCTGCACCAT+AGG 0.511417 2.1:+55262058 None:intergenic
GGTGCACAAATTAATCTTGA+AGG 0.512636 2.1:-55261939 MS.gene073453:CDS
AAGGATTGTGAATTCTTACA+TGG 0.512683 2.1:-55260899 MS.gene073453:CDS
ACCGTTGGCTGCATCTGCTA+TGG 0.521251 2.1:+55262004 None:intergenic
CATTGTTACAATAAGGCCAC+TGG 0.522380 2.1:-55260970 MS.gene073453:CDS
ATGGAAGGCATCAGTAACCT+TGG 0.524813 2.1:-55261963 MS.gene073453:CDS
ACTTTGGACTAAAATTCCAT+TGG 0.525845 2.1:+55261185 None:intergenic
GTCGCGAAAAGGTCGTTGCA+AGG 0.526188 2.1:-55260622 MS.gene073453:CDS
TGTTTGTTTCATGCTTCTTG+AGG 0.528462 2.1:-55262436 None:intergenic
TCAAAATTGTAGGATGATGA+AGG 0.529691 2.1:+55261321 None:intergenic
TCCTTCTTCTCAGCCAGCTG+AGG 0.533497 2.1:-55262247 MS.gene073453:intron
AGGTAGTTTCAAATAAATTC+CGG 0.535034 2.1:+55260936 None:intergenic
AAACACGAAAGGAATTTCTC+AGG 0.538491 2.1:-55261102 MS.gene073453:CDS
AGTTACCGGTAACCAGCGTT+GGG 0.539499 2.1:-55261887 MS.gene073453:CDS
AAGTTACCGGTAACCAGCGT+TGG 0.543200 2.1:-55261888 MS.gene073453:CDS
TGGATTCAAACTTTAGAGGA+GGG 0.543439 2.1:-55261272 MS.gene073453:CDS
TGACAGCATTATCGTTACCT+TGG 0.545887 2.1:+55261064 None:intergenic
GATTAATTTGTGCACCTCCA+AGG 0.547641 2.1:+55261946 None:intergenic
GATTCAAACTTTAGAGGAGG+GGG 0.548301 2.1:-55261270 MS.gene073453:CDS
ATGTTCTCTGGATCAAATCA+AGG 0.548900 2.1:-55260748 MS.gene073453:CDS
GTATACCATGTGACATCCTA+TGG 0.554929 2.1:-55262074 MS.gene073453:CDS
GCCATAGCAGATGCAGCCAA+CGG 0.558955 2.1:-55262005 MS.gene073453:CDS
GCTGGCTGAGAAGAAGGAGA+AGG 0.560665 2.1:+55262252 None:intergenic
GGAATTTATTTGAAACTACC+TGG 0.560862 2.1:-55260934 MS.gene073453:CDS
TCCTATGGTGCAGACCCTAC+AGG 0.561285 2.1:-55262059 MS.gene073453:CDS
GAAGGTGCAAAAGAAGATGA+TGG 0.564254 2.1:+55262270 None:intergenic
GGATTCAAACTTTAGAGGAG+GGG 0.565517 2.1:-55261271 MS.gene073453:CDS
GAAAGGAATTTCTCAGGCAC+AGG 0.566208 2.1:-55261096 MS.gene073453:CDS
TGTTGATAGGAACGTTGCAA+CGG 0.568090 2.1:-55260665 MS.gene073453:CDS
TTGGATTCAAACTTTAGAGG+AGG 0.571436 2.1:-55261273 MS.gene073453:CDS
GCACAAATTAATCTTGAAGG+TGG 0.580445 2.1:-55261936 MS.gene073453:CDS
TCGCGAAAAGGTCGTTGCAA+GGG 0.583554 2.1:-55260621 MS.gene073453:CDS
TACCATGATGTTCCCAACGC+TGG 0.586939 2.1:+55261875 None:intergenic
AGCGAAGGATATTTGATGGA+AGG 0.588639 2.1:-55261978 MS.gene073453:CDS
CTTGGCCAACACATAACCGC+CGG 0.589091 2.1:-55261132 MS.gene073453:CDS
GAAGGCATCAGTAACCTTGG+AGG 0.592934 2.1:-55261960 MS.gene073453:CDS
ATCCTTGTTTGTGTTAAACC+AGG 0.593807 2.1:+55260916 None:intergenic
CGGCGGTTATGTGTTGGCCA+AGG 0.595647 2.1:+55261133 None:intergenic
AGAGAACATGTTATCTACAA+TGG 0.597546 2.1:+55260762 None:intergenic
TCATGCTATCAGAAATGTGT+CGG 0.600119 2.1:-55260494 MS.gene073453:CDS
TCCGGTACCGCCGAATCCAG+TGG 0.602767 2.1:+55260954 None:intergenic
GCAGCCAACGGTCCAAGCGA+AGG 0.606415 2.1:-55261993 MS.gene073453:CDS
GGCACAGGAATAAACATCCA+AGG 0.625123 2.1:-55261081 MS.gene073453:CDS
AATAAGGCCACTGGATTCGG+CGG 0.629975 2.1:-55260961 MS.gene073453:CDS
TACCTGGTTTAACACAAACA+AGG 0.631189 2.1:-55260918 MS.gene073453:CDS
AACCAGCGTTGGGAACATCA+TGG 0.637299 2.1:-55261877 MS.gene073453:intron
AGCAGAGAAAATTACAACAT+CGG 0.644559 2.1:+55261041 None:intergenic
CTGCACCATAGGATGTCACA+TGG 0.645840 2.1:+55262069 None:intergenic
ATTGTGTTGAAATCTATGAA+AGG 0.648167 2.1:-55260802 MS.gene073453:CDS
GGCCAACACATAACCGCCGG+AGG 0.651132 2.1:-55261129 MS.gene073453:CDS
TTCGTGTTTATCGCCTCCGG+CGG 0.651957 2.1:+55261116 None:intergenic
CCGGAGGCGATAAACACGAA+AGG 0.669288 2.1:-55261113 MS.gene073453:CDS
ACACAATATTCGCATCACCA+AGG 0.694010 2.1:+55260818 None:intergenic
TTGATAGGAACGTTGCAACG+GGG 0.722305 2.1:-55260663 MS.gene073453:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACTAATTATTTGACAAT+TGG - chr2.1:55261150-55261169 MS.gene073453:CDS 15.0%
!! TTTCATACTGTAAAAAAAAA+AGG + chr2.1:55261111-55261130 None:intergenic 15.0%
!!! TTTTTGTTGTTTTAATTTGA+TGG - chr2.1:55261389-55261408 MS.gene073453:CDS 15.0%
!! AAGTTTGTCATATTTATTGT+CGG - chr2.1:55260685-55260704 MS.gene073453:CDS 20.0%
!! ACAATAAATATGACAAACTT+AGG + chr2.1:55260685-55260704 None:intergenic 20.0%
!! ATTGTCTCAAAAATTGAATT+TGG + chr2.1:55261202-55261221 None:intergenic 20.0%
!!! ATAATTAGTTTTTCTACAGT+TGG + chr2.1:55261142-55261161 None:intergenic 20.0%
!!! TAATTTTAGTAGAGAATACT+AGG - chr2.1:55260638-55260657 MS.gene073453:CDS 20.0%
!!! TTTAGTATTTTTAGTCATGA+TGG - chr2.1:55261231-55261250 MS.gene073453:CDS 20.0%
! AAACTTAGGTACAATGTTAT+AGG + chr2.1:55260671-55260690 None:intergenic 25.0%
! AGGTAGTTTCAAATAAATTC+CGG + chr2.1:55261907-55261926 None:intergenic 25.0%
! AGTTATGTACTCAAAATTGT+AGG + chr2.1:55261532-55261551 None:intergenic 25.0%
! AGTTTCTTATGTATGTTTCA+TGG + chr2.1:55261683-55261702 None:intergenic 25.0%
! ATTGTGTTGAAATCTATGAA+AGG - chr2.1:55262038-55262057 MS.gene073453:CDS 25.0%
! GTTGTTTGTTTCATTGTAAT+TGG - chr2.1:55260426-55260445 MS.gene073453:CDS 25.0%
! TAAAGTTTGAATCCAATAAG+AGG + chr2.1:55261563-55261582 None:intergenic 25.0%
! TTATTGGATTCAAACTTTAG+AGG - chr2.1:55261564-55261583 MS.gene073453:intron 25.0%
! TTGATCAAGTTATTGTTGAT+AGG - chr2.1:55262162-55262181 MS.gene073453:intron 25.0%
!! ATAGTCAATTCTCATTTTCT+TGG + chr2.1:55261279-55261298 None:intergenic 25.0%
!! TGAACATTTTTGATAAGGTT+AGG + chr2.1:55262290-55262309 None:intergenic 25.0%
!! TTCATTGTAATTGGTGTTTA+TGG - chr2.1:55260435-55260454 MS.gene073453:CDS 25.0%
!!! AAAATCATCTGAAGCTTTTA+TGG + chr2.1:55261436-55261455 None:intergenic 25.0%
!!! AGTACTGAACATTTTTGATA+AGG + chr2.1:55262295-55262314 None:intergenic 25.0%
!!! GATTTTTTTGTTGTTGTGTA+GGG - chr2.1:55260727-55260746 MS.gene073453:CDS 25.0%
!!! TCTATGAAAGGTGTTTTAAA+TGG - chr2.1:55262050-55262069 MS.gene073453:CDS 25.0%
!!! TGATTTTTTTGTTGTTGTGT+AGG - chr2.1:55260726-55260745 MS.gene073453:CDS 25.0%
AAGGATTGTGAATTCTTACA+TGG - chr2.1:55261941-55261960 MS.gene073453:CDS 30.0%
ACATTTCTGATAGCATGATT+AGG + chr2.1:55262344-55262363 None:intergenic 30.0%
ACCTCATCAAATGAAAATGA+TGG - chr2.1:55261486-55261505 MS.gene073453:intron 30.0%
ACTTGATCAACTTGATGAAA+AGG + chr2.1:55262152-55262171 None:intergenic 30.0%
ACTTTGGACTAAAATTCCAT+TGG + chr2.1:55261658-55261677 None:intergenic 30.0%
AGAGAACATGTTATCTACAA+TGG + chr2.1:55262081-55262100 None:intergenic 30.0%
AGCAGAGAAAATTACAACAT+CGG + chr2.1:55261802-55261821 None:intergenic 30.0%
ATTACACATTTCACAACCAA+TGG - chr2.1:55261639-55261658 MS.gene073453:intron 30.0%
ATTGTAGATAACATGTTCTC+TGG - chr2.1:55262080-55262099 MS.gene073453:CDS 30.0%
ATTGTATTATATACCTCAGC+TGG + chr2.1:55260609-55260628 None:intergenic 30.0%
CTAGAAATATGAAGTTGAAC+AGG + chr2.1:55261993-55262012 None:intergenic 30.0%
GTGACAAAAATTTACCTACT+AGG - chr2.1:55260457-55260476 MS.gene073453:CDS 30.0%
TACATAAGAAACTCTTTCCT+TGG - chr2.1:55261690-55261709 MS.gene073453:intron 30.0%
TCAAAATTGTAGGATGATGA+AGG + chr2.1:55261522-55261541 None:intergenic 30.0%
TGGTGTACATTGTTACAATA+AGG - chr2.1:55261863-55261882 MS.gene073453:intron 30.0%
TTGATGGAAAAACAGATACA+TGG - chr2.1:55261405-55261424 MS.gene073453:CDS 30.0%
! AGAATCATGTCTTGTTAAAG+AGG + chr2.1:55260538-55260557 None:intergenic 30.0%
! GGAATTTATTTGAAACTACC+TGG - chr2.1:55261906-55261925 MS.gene073453:CDS 30.0%
! TCAGATGATTTTCCAAATGA+TGG - chr2.1:55261444-55261463 MS.gene073453:intron 30.0%
! TCCATCATTTTCATTTGATG+AGG + chr2.1:55261490-55261509 None:intergenic 30.0%
!! AATGGAATTTTAGTCCAAAG+TGG - chr2.1:55261657-55261676 MS.gene073453:intron 30.0%
!! AGGTGTTGAAATTGTTCATT+TGG - chr2.1:55262112-55262131 MS.gene073453:intron 30.0%
!! ATTTCTAGTAGTTTCTTCCT+TGG - chr2.1:55262005-55262024 MS.gene073453:CDS 30.0%
!! TTTTGAGTACATAACTCTCA+AGG - chr2.1:55261536-55261555 MS.gene073453:intron 30.0%
!!! GGAAGTGTTTTAAGACTTTA+TGG - chr2.1:55261171-55261190 MS.gene073453:CDS 30.0%
!!! TGTTTTAAGACTTTATGGCT+AGG - chr2.1:55261176-55261195 MS.gene073453:CDS 30.0%
AAACACGAAAGGAATTTCTC+AGG - chr2.1:55261738-55261757 MS.gene073453:intron 35.0%
AGCAAATGAAACAATTTCGC+AGG + chr2.1:55260993-55261012 None:intergenic 35.0%
ATGGAAAAACAGATACATGG+AGG - chr2.1:55261408-55261427 MS.gene073453:CDS 35.0%
ATGTTCTCTGGATCAAATCA+AGG - chr2.1:55262092-55262111 MS.gene073453:CDS 35.0%
CAGTACTCATTAAGTTCAAC+AGG - chr2.1:55262308-55262327 MS.gene073453:CDS 35.0%
CCTACTAGGAAAATTTCTGA+TGG - chr2.1:55260471-55260490 MS.gene073453:CDS 35.0%
GCACAAATTAATCTTGAAGG+TGG - chr2.1:55260904-55260923 MS.gene073453:CDS 35.0%
GGTGCACAAATTAATCTTGA+AGG - chr2.1:55260901-55260920 MS.gene073453:CDS 35.0%
TACCTGGTTTAACACAAACA+AGG - chr2.1:55261922-55261941 MS.gene073453:CDS 35.0%
TCAATGATGTAGCCATCATT+TGG + chr2.1:55261459-55261478 None:intergenic 35.0%
TCATGCTATCAGAAATGTGT+CGG - chr2.1:55262346-55262365 MS.gene073453:CDS 35.0%
TGCTATCTGAATTTCCTGTA+GGG + chr2.1:55260798-55260817 None:intergenic 35.0%
! ATCCTTGTTTGTGTTAAACC+AGG + chr2.1:55261927-55261946 None:intergenic 35.0%
! CCATCAGAAATTTTCCTAGT+AGG + chr2.1:55260474-55260493 None:intergenic 35.0%
! GTAATTTTCTCTGCTGCTAT+AGG - chr2.1:55261807-55261826 MS.gene073453:intron 35.0%
! TGGATTCAAACTTTAGAGGA+GGG - chr2.1:55261568-55261587 MS.gene073453:intron 35.0%
! TTGGATTCAAACTTTAGAGG+AGG - chr2.1:55261567-55261586 MS.gene073453:intron 35.0%
AACTCTCAAGGACCTCTTAT+TGG - chr2.1:55261548-55261567 MS.gene073453:intron 40.0%
ACACAATATTCGCATCACCA+AGG + chr2.1:55262025-55262044 None:intergenic 40.0%
AGCGAAGGATATTTGATGGA+AGG - chr2.1:55260862-55260881 MS.gene073453:CDS 40.0%
CAGAAAGTCACTCAAGTTAC+CGG - chr2.1:55260939-55260958 MS.gene073453:CDS 40.0%
CATTGTTACAATAAGGCCAC+TGG - chr2.1:55261870-55261889 MS.gene073453:intron 40.0%
GATGTCACATGGTATACTCT+TGG + chr2.1:55260763-55260782 None:intergenic 40.0%
GCTATAGGAATCATGGTAAC+TGG - chr2.1:55261822-55261841 MS.gene073453:intron 40.0%
GTATACCATGTGACATCCTA+TGG - chr2.1:55260766-55260785 MS.gene073453:CDS 40.0%
GTGCTATCTGAATTTCCTGT+AGG + chr2.1:55260799-55260818 None:intergenic 40.0%
TACAATAAGGCCACTGGATT+CGG - chr2.1:55261876-55261895 MS.gene073453:intron 40.0%
TCCAAGCGAAGGATATTTGA+TGG - chr2.1:55260858-55260877 MS.gene073453:CDS 40.0%
TCCATCAAATATCCTTCGCT+TGG + chr2.1:55260862-55260881 None:intergenic 40.0%
TGACAGCATTATCGTTACCT+TGG + chr2.1:55261779-55261798 None:intergenic 40.0%
TGCAACGGGGATGAATTTAA+AGG - chr2.1:55262190-55262209 MS.gene073453:intron 40.0%
TGTATGTTTCATGGCCACTT+TGG + chr2.1:55261674-55261693 None:intergenic 40.0%
! ACTGTAGAAACTGATTCTCG+AGG - chr2.1:55261055-55261074 MS.gene073453:CDS 40.0%
! AGTCATGATGGTGAAGTAGT+TGG - chr2.1:55261243-55261262 MS.gene073453:CDS 40.0%
! GAAGGTGCAAAAGAAGATGA+TGG + chr2.1:55260573-55260592 None:intergenic 40.0%
! GATTCAAACTTTAGAGGAGG+GGG - chr2.1:55261570-55261589 MS.gene073453:intron 40.0%
! GGATTCAAACTTTAGAGGAG+GGG - chr2.1:55261569-55261588 MS.gene073453:intron 40.0%
! GGTCAAGCAAACACATTTTC+TGG - chr2.1:55261843-55261862 MS.gene073453:intron 40.0%
! TGTTGATAGGAACGTTGCAA+CGG - chr2.1:55262175-55262194 MS.gene073453:intron 40.0%
!! GATTAATTTGTGCACCTCCA+AGG + chr2.1:55260897-55260916 None:intergenic 40.0%
AAAAGGTCGTTGCAAGGGAA+TGG - chr2.1:55262224-55262243 MS.gene073453:intron 45.0%
CTCTGCTGCTATAGGAATCA+TGG - chr2.1:55261815-55261834 MS.gene073453:intron 45.0%
GAAAGGAATTTCTCAGGCAC+AGG - chr2.1:55261744-55261763 MS.gene073453:intron 45.0%
GGCACAGGAATAAACATCCA+AGG - chr2.1:55261759-55261778 MS.gene073453:intron 45.0%
TAAAGGCTACTGTCGCGAAA+AGG - chr2.1:55262207-55262226 MS.gene073453:intron 45.0%
TGCAAGGGAATGGAACTTCA+TGG - chr2.1:55262234-55262253 MS.gene073453:intron 45.0%
TTGATAGGAACGTTGCAACG+GGG - chr2.1:55262177-55262196 MS.gene073453:intron 45.0%
! GTTGATAGGAACGTTGCAAC+GGG - chr2.1:55262176-55262195 MS.gene073453:intron 45.0%
!! ATGGAAGGCATCAGTAACCT+TGG - chr2.1:55260877-55260896 MS.gene073453:CDS 45.0%
!! TGCTGCGAATGTTTTTGTCG+CGG - chr2.1:55262403-55262422 MS.gene073453:CDS 45.0%
AAGTTACCGGTAACCAGCGT+TGG - chr2.1:55260952-55260971 MS.gene073453:CDS 50.0%
AATAAGGCCACTGGATTCGG+CGG - chr2.1:55261879-55261898 MS.gene073453:CDS 50.0%
AGTTACCGGTAACCAGCGTT+GGG - chr2.1:55260953-55260972 MS.gene073453:CDS 50.0%
ATATCCTTCGCTTGGACCGT+TGG + chr2.1:55260854-55260873 None:intergenic 50.0%
CCTTTCGTGTTTATCGCCTC+CGG + chr2.1:55261730-55261749 None:intergenic 50.0%
CTGCACCATAGGATGTCACA+TGG + chr2.1:55260774-55260793 None:intergenic 50.0%
TACCATGATGTTCCCAACGC+TGG + chr2.1:55260968-55260987 None:intergenic 50.0%
TCGCGAAAAGGTCGTTGCAA+GGG - chr2.1:55262219-55262238 MS.gene073453:intron 50.0%
! AACCAGCGTTGGGAACATCA+TGG - chr2.1:55260963-55260982 MS.gene073453:CDS 50.0%
! ATGTTCCCAACGCTGGTTAC+CGG + chr2.1:55260961-55260980 None:intergenic 50.0%
!! GAAGGCATCAGTAACCTTGG+AGG - chr2.1:55260880-55260899 MS.gene073453:CDS 50.0%
!! TGCGAATGTTTTTGTCGCGG+TGG - chr2.1:55262406-55262425 MS.gene073453:CDS 50.0%
ACCTCAGCTGGCTGAGAAGA+AGG + chr2.1:55260597-55260616 None:intergenic 55.0%
CCGGAGGCGATAAACACGAA+AGG - chr2.1:55261727-55261746 MS.gene073453:intron 55.0%
CTTGGCCAACACATAACCGC+CGG - chr2.1:55261708-55261727 MS.gene073453:intron 55.0%
GCCATAGCAGATGCAGCCAA+CGG - chr2.1:55260835-55260854 MS.gene073453:CDS 55.0%
GCTGGCTGAGAAGAAGGAGA+AGG + chr2.1:55260591-55260610 None:intergenic 55.0%
GTCGCGAAAAGGTCGTTGCA+AGG - chr2.1:55262218-55262237 MS.gene073453:intron 55.0%
TCCTATGGTGCAGACCCTAC+AGG - chr2.1:55260781-55260800 MS.gene073453:CDS 55.0%
TCCTGTAGGGTCTGCACCAT+AGG + chr2.1:55260785-55260804 None:intergenic 55.0%
TCCTTCTTCTCAGCCAGCTG+AGG - chr2.1:55260593-55260612 MS.gene073453:CDS 55.0%
TTCGTGTTTATCGCCTCCGG+CGG + chr2.1:55261727-55261746 None:intergenic 55.0%
! ACCGTTGGCTGCATCTGCTA+TGG + chr2.1:55260839-55260858 None:intergenic 55.0%
! CGGCGGTTATGTGTTGGCCA+AGG + chr2.1:55261710-55261729 None:intergenic 60.0%
CGCCTCCGGCGGTTATGTGT+TGG + chr2.1:55261716-55261735 None:intergenic 65.0%
GCAGCCAACGGTCCAAGCGA+AGG - chr2.1:55260847-55260866 MS.gene073453:CDS 65.0%
GCCACTGGATTCGGCGGTAC+CGG - chr2.1:55261885-55261904 MS.gene073453:CDS 65.0%
GGCCAACACATAACCGCCGG+AGG - chr2.1:55261711-55261730 MS.gene073453:intron 65.0%
TCCGGTACCGCCGAATCCAG+TGG + chr2.1:55261889-55261908 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 55260414 55262448 55260414 ID=MS.gene073453
chr2.1 mRNA 55260414 55262448 55260414 ID=MS.gene073453.t1;Parent=MS.gene073453
chr2.1 exon 55262248 55262448 55262248 ID=MS.gene073453.t1.exon1;Parent=MS.gene073453.t1
chr2.1 CDS 55262248 55262448 55262248 ID=cds.MS.gene073453.t1;Parent=MS.gene073453.t1
chr2.1 exon 55261878 55262114 55261878 ID=MS.gene073453.t1.exon2;Parent=MS.gene073453.t1
chr2.1 CDS 55261878 55262114 55261878 ID=cds.MS.gene073453.t1;Parent=MS.gene073453.t1
chr2.1 exon 55260414 55261442 55260414 ID=MS.gene073453.t1.exon3;Parent=MS.gene073453.t1
chr2.1 CDS 55260414 55261442 55260414 ID=cds.MS.gene073453.t1;Parent=MS.gene073453.t1
Gene Sequence

>MS.gene073453

ATGCTTCTTGAGGTTGTTTGTTTCATTGTAATTGGTGTTTATGGTGACAAAAATTTACCTACTAGGAAAATTTCTGATGGAAATTATCATGAAACAATACGAAGATTGCAATCATTTAAAGCCTCTTTAACAAGACATGATTCTATTGTTTCAACACCATCATCTTCTTTTGCACCTTCTCCTTCTTCTCAGCCAGCTGAGGTATATAATACAATCATATACGATAATTTTAGTAGAGAATACTAGGAACAATGCCTATAACATTGTACCTAAGTTTGTCATATTTATTGTCGGAAAATATCGAATGTTTGATGATTTTTTTGTTGTTGTGTAGGGTATGAACAATCCAAGAGTATACCATGTGACATCCTATGGTGCAGACCCTACAGGAAATTCAGATAGCACTGAAGCATTACTTGCAGCCATAGCAGATGCAGCCAACGGTCCAAGCGAAGGATATTTGATGGAAGGCATCAGTAACCTTGGAGGTGCACAAATTAATCTTGAAGGTGGAAATTACATGATCAGAAAGTCACTCAAGTTACCGGTAACCAGCGTTGGGAACATCATGGTACGCCTGCGAAATTGTTTCATTTGCTAATTACATAAACAAAACTATAAATCAAATATCTCTGCGTACAACTGTAGAAACTGATTCTCGAGGATGCACAACTGTAGAAAAATTGCATGCTGTCCTTTTTTTTTTACAGTATGAAAAGTATCTACCAACTGTAGAAAAACTAATTATTTGACAATTGGAAGTGTTTTAAGACTTTATGGCTAGGCCAAATTCAATTTTTGAGACAATTAGTCACAATTTAGTATTTTTAGTCATGATGGTGAAGTAGTTGGTCTAACTAGACCAAGAAAATGAGAATTGACTATTTCTATTAACATATTCAAGTTTTTGTTTTTCATTTGTTTATTCAATAAAGCATTCAAAAGAAATCAAAATTTTTGACATGTTAATTTGATTTTTTGTTGTTTTAATTTGATGGAAAAACAGATACATGGAGGAACCATAAAAGCTTCAGATGATTTTCCAAATGATGGCTACATCATTGATTTGTCAACCTCATCAAATGAAAATGATGGAAAAAATTCACCTTCATCATCCTACAATTTTGAGTACATAACTCTCAAGGACCTCTTATTGGATTCAAACTTTAGAGGAGGGGGCATTTCAGTCATAAACTCACTCAGAACAAACATAGACAATTGTTACATTACACATTTCACAACCAATGGAATTTTAGTCCAAAGTGGCCATGAAACATACATAAGAAACTCTTTCCTTGGCCAACACATAACCGCCGGAGGCGATAAACACGAAAGGAATTTCTCAGGCACAGGAATAAACATCCAAGGTAACGATAATGCTGTCACCGATGTTGTAATTTTCTCTGCTGCTATAGGAATCATGGTAACTGGTCAAGCAAACACATTTTCTGGTGTACATTGTTACAATAAGGCCACTGGATTCGGCGGTACCGGAATTTATTTGAAACTACCTGGTTTAACACAAACAAGGATTGTGAATTCTTACATGGATTACACAAGTATTGTTGCTGAAGATCCTGTTCAACTTCATATTTCTAGTAGTTTCTTCCTTGGTGATGCGAATATTGTGTTGAAATCTATGAAAGGTGTTTTAAATGGTGTTACCATTGTAGATAACATGTTCTCTGGATCAAATCAAGGTGTTGAAATTGTTCATTTGGATAAATCAAACAGTCCTTTTCATCAAGTTGATCAAGTTATTGTTGATAGGAACGTTGCAACGGGGATGAATTTAAAGGCTACTGTCGCGAAAAGGTCGTTGCAAGGGAATGGAACTTCATGGAATGTTGATTTTAACAACATTCTTCTTTTTCCTAACCTTATCAAAAATGTTCAGTACTCATTAAGTTCAACAGGTAGCAGTTTTCCTAATCATGCTATCAGAAATGTGTCGGACAATCGCGTTGTTATTGAAACGAATGAAGCAGTTGCTGCGAATGTTTTTGTCGCGGTGGATCAAAGTATGTCAAGTTGA
Protein sequence

>MS.gene073453.t1

MLLEVVCFIVIGVYGDKNLPTRKISDGNYHETIRRLQSFKASLTRHDSIVSTPSSSFAPSPSSQPAEGMNNPRVYHVTSYGADPTGNSDSTEALLAAIADAANGPSEGYLMEGISNLGGAQINLEGGNYMIRKSLKLPVTSVGNIMIHGGTIKASDDFPNDGYIIDLSTSSNENDGKNSPSSSYNFEYITLKDLLLDSNFRGGGISVINSLRTNIDNCYITHFTTNGILVQSGHETYIRNSFLGQHITAGGDKHERNFSGTGINIQGNDNAVTDVVIFSAAIGIMVTGQANTFSGVHCYNKATGFGGTGIYLKLPGLTQTRIVNSYMDYTSIVAEDPVQLHISSSFFLGDANIVLKSMKGVLNGVTIVDNMFSGSNQGVEIVHLDKSNSPFHQVDQVIVDRNVATGMNLKATVAKRSLQGNGTSWNVDFNNILLFPNLIKNVQYSLSSTGSSFPNHAIRNVSDNRVVIETNEAVAANVFVAVDQSMSS