AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene073458

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073458.t1 MTR_2g035790 91.051 447 29 1 1 436 42 488 0.0 791
MS.gene073458.t1 MTR_4g094732 71.462 431 118 3 9 436 52 480 0.0 615
MS.gene073458.t1 MTR_2g035760 52.300 413 179 7 17 424 82 481 5.85e-140 410
MS.gene073458.t1 MTR_6g015785 52.103 428 181 8 13 436 83 490 1.16e-133 394
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073458.t1 AT4G20040 63.850 426 147 5 13 436 63 483 0.0 550
MS.gene073458.t1 AT4G20050 54.118 425 171 6 17 436 74 479 1.41e-149 434
MS.gene073458.t1 AT4G20050 54.118 425 171 6 17 436 74 479 1.41e-149 434
MS.gene073458.t1 AT4G20050 54.118 425 171 6 17 436 74 479 1.41e-149 434
MS.gene073458.t1 AT4G20050 54.118 425 171 6 17 436 74 479 1.41e-149 434
MS.gene073458.t1 AT4G20050 54.118 425 171 6 17 436 99 504 2.15e-149 434
MS.gene073458.t1 AT4G20050 53.750 400 161 6 42 436 1 381 6.42e-138 400
MS.gene073458.t1 AT4G20050 53.750 400 161 6 42 436 1 381 6.42e-138 400

Find 85 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATTTGATATTGATAATTT+AGG 0.107857 2.1:-55226434 MS.gene073458:CDS
ACAATTGGAACATTATTATT+AGG 0.129299 2.1:+55223815 None:intergenic
TCAATTAAAGGGAAAGTTTA+TGG 0.147921 2.1:-55223872 MS.gene073458:CDS
GGATCTGCACCATACTTAAT+TGG 0.153680 2.1:+55226506 None:intergenic
CATTTGATATTGATAATTTA+GGG 0.251344 2.1:-55226433 MS.gene073458:CDS
GTTCCAATTGTGAAATTAGA+TGG 0.269638 2.1:-55223803 MS.gene073458:CDS
CATCAATGACCAAAGGTAAA+TGG 0.278889 2.1:-55224544 MS.gene073458:intron
CAATTGGAACATTATTATTA+GGG 0.285274 2.1:+55223816 None:intergenic
TATAACAAAGCAACAGGTTT+TGG 0.286379 2.1:-55224046 MS.gene073458:CDS
ATTGTTAACAATATGTTTAG+TGG 0.301676 2.1:-55223842 MS.gene073458:CDS
TGAAAATGACTAATCCTATT+AGG 0.302520 2.1:+55223641 None:intergenic
CAGCCATAGGAATTGTTGTT+AGG 0.306110 2.1:-55224102 MS.gene073458:CDS
TGGATGGTAATGGCACAAAA+TGG 0.367127 2.1:-55223694 MS.gene073458:CDS
AAATGTTTCAGAAGCTCTTA+AGG 0.373297 2.1:+55224516 None:intergenic
TACTTGATATGAAGGTTTCA+TGG 0.388165 2.1:+55224242 None:intergenic
CCTCCAAGGTCTCTAATTCC+TGG 0.397675 2.1:+55226398 None:intergenic
CATTGTTATAACAAAGCAAC+AGG 0.404226 2.1:-55224052 MS.gene073458:CDS
TAATTCCTTCGGTGGTGAAA+TGG 0.411730 2.1:+55224278 None:intergenic
ACTTTGAACTTTAATTCCTT+CGG 0.420815 2.1:+55224267 None:intergenic
TTCGTAACATGAACTTGAAC+AGG 0.426663 2.1:+55223932 None:intergenic
TTGTTCAAGTAATCTGTTGT+TGG 0.430783 2.1:+55224423 None:intergenic
AATTCATTGTTCAACAAAGA+AGG 0.431005 2.1:+55223508 None:intergenic
ACTGAATGTGCAACAAACTT+TGG 0.434126 2.1:+55223593 None:intergenic
AAGAAAACTACTTGATATGA+AGG 0.434177 2.1:+55224234 None:intergenic
AGCTTCTACAGCTTTCATAA+TGG 0.438110 2.1:+55226457 None:intergenic
AAGTATGGTGCAGATCCAAC+AGG 0.439437 2.1:-55226500 MS.gene073458:CDS
ATATTCATTTCATGCATTAG+AGG 0.439787 2.1:-55223618 MS.gene073458:CDS
GATCTGCACCATACTTAATT+GGG 0.441924 2.1:+55226507 None:intergenic
ATCACATTTCCTTCATCGGC+TGG 0.454793 2.1:-55226329 MS.gene073458:CDS
AGATTACTTGAACAAACAAT+AGG 0.463517 2.1:-55224415 MS.gene073458:CDS
TCCCATCACATTTCCTTCAT+CGG 0.471050 2.1:-55226333 MS.gene073458:CDS
AGCCATAGGAATTGTTGTTA+GGG 0.472547 2.1:-55224101 MS.gene073458:CDS
GAAAGTGAGAAACCAGTTCA+TGG 0.473042 2.1:-55223539 MS.gene073458:CDS
TTCCAATTGTGAAATTAGAT+GGG 0.473930 2.1:-55223802 MS.gene073458:CDS
AAGGAAACAACCCCATGAAC+TGG 0.475950 2.1:+55223527 None:intergenic
TTACCAGGAATTAGAGACCT+TGG 0.494991 2.1:-55226401 MS.gene073458:CDS
TCCCCTAACAACAATTCCTA+TGG 0.501817 2.1:+55224099 None:intergenic
TATTCATTTCATGCATTAGA+GGG 0.507592 2.1:-55223617 MS.gene073458:CDS
AAGAATTGATAATTGTTACA+TGG 0.510868 2.1:-55223981 MS.gene073458:CDS
GTCAAATAATGTTGTAGTTG+TGG 0.512201 2.1:-55223561 MS.gene073458:CDS
TTAGGGCTTGAATTATTACC+AGG 0.514044 2.1:-55226416 MS.gene073458:CDS
TTCCTCCATTTCACCACCGA+AGG 0.521417 2.1:-55224283 MS.gene073458:CDS
TCAACAAGGTGTCTATCAGT+TGG 0.524915 2.1:+55224493 None:intergenic
GTGGTGTACCCAATTAAGTA+TGG 0.526549 2.1:-55226515 MS.gene073458:CDS
ACAAGATTGCCAGCCGATGA+AGG 0.526613 2.1:+55226320 None:intergenic
CTTGGACAACACTCAACAGT+CGG 0.527472 2.1:-55224214 MS.gene073458:CDS
GTTCAAGTTCATGTTACGAA+TGG 0.528355 2.1:-55223929 MS.gene073458:CDS
GATTCAAGTTACCGAGGTGG+AGG 0.539152 2.1:-55224352 MS.gene073458:CDS
TTGAACTTTAATTCCTTCGG+TGG 0.542054 2.1:+55224270 None:intergenic
ATTGATATGAACAATGTGAT+TGG 0.543536 2.1:-55223752 MS.gene073458:CDS
TGGTAATGGCACAAAATGGG+TGG 0.549729 2.1:-55223690 MS.gene073458:CDS
GGATGGTAATGGCACAAAAT+GGG 0.551911 2.1:-55223693 MS.gene073458:CDS
GGATTATAATTCTATAGTCA+TGG 0.563335 2.1:-55223960 MS.gene073458:CDS
AAAGTGAGAAACCAGTTCAT+GGG 0.566325 2.1:-55223538 MS.gene073458:CDS
TTCATCGGCTGGCAATCTTG+TGG 0.566505 2.1:-55226318 MS.gene073458:intron
TCAACAGTCGGCGGAGACAA+AGG 0.569193 2.1:-55224202 MS.gene073458:CDS
AATGAAGAAGAGTGGAAGAG+TGG 0.571785 2.1:-55226534 MS.gene073458:intron
TTCCTTCGGTGGTGAAATGG+AGG 0.577526 2.1:+55224281 None:intergenic
GCCGATGAAGGAAATGTGAT+GGG 0.577733 2.1:+55226332 None:intergenic
CCAGGAATTAGAGACCTTGG+AGG 0.587401 2.1:-55226398 MS.gene073458:CDS
GGACAAGCTAACATGATCAC+AGG 0.590351 2.1:-55224079 MS.gene073458:CDS
ATTTAGTGACATTGATCAAG+TGG 0.593403 2.1:-55223777 MS.gene073458:CDS
TCCAATTGTGAAATTAGATG+GGG 0.603106 2.1:-55223801 MS.gene073458:CDS
CTCGGTAACTTGAATCGAAG+AGG 0.611266 2.1:+55224359 None:intergenic
AGCCGATGAAGGAAATGTGA+TGG 0.612995 2.1:+55226331 None:intergenic
AAGTTGAATGTGGATGGTAA+TGG 0.619073 2.1:-55223704 MS.gene073458:CDS
AATCAATGATAACACCTCCA+AGG 0.627415 2.1:+55226384 None:intergenic
AACCGTTGGGAAGTTGAATG+TGG 0.628722 2.1:-55223714 MS.gene073458:CDS
TGCTGAGAAAATTGCAACAT+CGG 0.630023 2.1:+55224123 None:intergenic
GTGTGAAGATGGCTGAGACA+TGG 0.634929 2.1:+55226828 None:intergenic
TTCGATTCAAGTTACCGAGG+TGG 0.636613 2.1:-55224355 MS.gene073458:CDS
GCCATAGGAATTGTTGTTAG+GGG 0.637301 2.1:-55224100 MS.gene073458:CDS
CCAATTGTGAAATTAGATGG+GGG 0.638303 2.1:-55223800 MS.gene073458:CDS
GGTAATGGCACAAAATGGGT+GGG 0.641219 2.1:-55223689 MS.gene073458:CDS
AGTATGGTGCAGATCCAACA+GGG 0.649273 2.1:-55226499 MS.gene073458:CDS
ATCCACATTCAACTTCCCAA+CGG 0.649744 2.1:+55223712 None:intergenic
TTGATGCATTTAATTCAACA+AGG 0.650431 2.1:+55224479 None:intergenic
AAGTGAGAAACCAGTTCATG+GGG 0.654495 2.1:-55223537 MS.gene073458:CDS
GTTGGGAAGTTGAATGTGGA+TGG 0.656072 2.1:-55223710 MS.gene073458:CDS
GAAGGAATTAAAGTTCAAAG+TGG 0.662811 2.1:-55224265 MS.gene073458:CDS
CAATGACCAAAGGTAAATGG+AGG 0.667241 2.1:-55224541 MS.gene073458:intron
CTCTTCGATTCAAGTTACCG+AGG 0.668555 2.1:-55224358 MS.gene073458:CDS
GGACAACACTCAACAGTCGG+CGG 0.701448 2.1:-55224211 MS.gene073458:CDS
CGATGAATATTCCTCCACCT+CGG 0.718566 2.1:+55224341 None:intergenic
GTATGGTGCAGATCCAACAG+GGG 0.763386 2.1:-55226498 MS.gene073458:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAGAAAAATATTTATTTAT+AGG + chr2.1:55224643-55224662 None:intergenic 10.0%
!! CTAATTTATATAACAAATTA+AGG + chr2.1:55224156-55224175 None:intergenic 10.0%
!! TCAAAATAATAAATTTGTAT+TGG - chr2.1:55225266-55225285 MS.gene073458:intron 10.0%
!!! ATGAGATTTTAAAAAAATTA+TGG - chr2.1:55224939-55224958 MS.gene073458:intron 10.0%
!!! GAATTTTTTTTTTTTGAATA+AGG - chr2.1:55225131-55225150 MS.gene073458:intron 10.0%
!!! TTTTGATAATATTTATTATG+AGG - chr2.1:55224546-55224565 MS.gene073458:intron 10.0%
!!! TTTTTTTTGATGATTTTAAT+TGG - chr2.1:55223606-55223625 MS.gene073458:CDS 10.0%
!! AAATAAAACATTATTTGGAA+TGG + chr2.1:55224512-55224531 None:intergenic 15.0%
!! AAATCATAACATAACAATAT+TGG - chr2.1:55225680-55225699 MS.gene073458:intron 15.0%
!! AATAATATGTAAAAGTTAAG+TGG + chr2.1:55225764-55225783 None:intergenic 15.0%
!! TGTGAATTTAATGATAAATA+AGG + chr2.1:55225483-55225502 None:intergenic 15.0%
!!! AATAAAACATTATTTGGAAT+GGG + chr2.1:55224511-55224530 None:intergenic 15.0%
!!! ATTGTTTTGAAATCAATTAA+AGG - chr2.1:55226465-55226484 MS.gene073458:CDS 15.0%
!!! CATTTGATATTGATAATTTA+GGG - chr2.1:55223916-55223935 MS.gene073458:CDS 15.0%
!!! CATTTTATACAATGAAAAAA+AGG + chr2.1:55223752-55223771 None:intergenic 15.0%
!!! TCAAATTTAATTTGTAAACA+TGG - chr2.1:55225055-55225074 MS.gene073458:intron 15.0%
!!! TCTCTTTTGATTTTTTTTAT+TGG - chr2.1:55224658-55224677 MS.gene073458:intron 15.0%
!!! TTGTTTTGAAATCAATTAAA+GGG - chr2.1:55226466-55226485 MS.gene073458:CDS 15.0%
!! AATCATCAAAAAAAATTCCT+AGG + chr2.1:55223601-55223620 None:intergenic 20.0%
!! ACAATTGGAACATTATTATT+AGG + chr2.1:55226537-55226556 None:intergenic 20.0%
!! ACATCTACTTTAATAGTTTA+TGG - chr2.1:55225521-55225540 MS.gene073458:intron 20.0%
!! ATTGTTAACAATATGTTTAG+TGG - chr2.1:55226507-55226526 MS.gene073458:CDS 20.0%
!! CAATTGGAACATTATTATTA+GGG + chr2.1:55226536-55226555 None:intergenic 20.0%
!! TCGATTCAATAGAATAAAAA+AGG - chr2.1:55224897-55224916 MS.gene073458:intron 20.0%
!!! AAGAATTGATAATTGTTACA+TGG - chr2.1:55226368-55226387 MS.gene073458:CDS 20.0%
!!! AGTTGCAAAATTTTATTGAA+AGG + chr2.1:55225340-55225359 None:intergenic 20.0%
!!! ATAAAACATTATTTGGAATG+GGG + chr2.1:55224510-55224529 None:intergenic 20.0%
!!! GCATTTGATATTGATAATTT+AGG - chr2.1:55223915-55223934 MS.gene073458:CDS 20.0%
! AAAAAAAATTCCTAGGAAGA+AGG + chr2.1:55223594-55223613 None:intergenic 25.0%
! AAACATCAAAAGAAAAGGAA+TGG - chr2.1:55225627-55225646 MS.gene073458:intron 25.0%
! AAACATCAAAAGAAAAGGAA+TGG + chr2.1:55225646-55225627 None:intergenic 25.0%
! AAGAAAACTACTTGATATGA+AGG + chr2.1:55226118-55226137 None:intergenic 25.0%
! AAGAGTTAAAACAAAGAAGA+GGG - chr2.1:55224360-55224379 MS.gene073458:CDS 25.0%
! ACTTTGAACTTTAATTCCTT+CGG + chr2.1:55226085-55226104 None:intergenic 25.0%
! AGATTACTTGAACAAACAAT+AGG - chr2.1:55225934-55225953 MS.gene073458:intron 25.0%
! AGCTAAAACATCAAAAGAAA+AGG + chr2.1:55225396-55225415 None:intergenic 25.0%
! ATATTCATTTCATGCATTAG+AGG - chr2.1:55226731-55226750 MS.gene073458:intron 25.0%
! ATTGATATGAACAATGTGAT+TGG - chr2.1:55226597-55226616 MS.gene073458:intron 25.0%
! CATAACATAACAATATTGGA+GGG - chr2.1:55225684-55225703 MS.gene073458:intron 25.0%
! GGATTATAATTCTATAGTCA+TGG - chr2.1:55226389-55226408 MS.gene073458:CDS 25.0%
! TAAGAGTTAAAACAAAGAAG+AGG - chr2.1:55224359-55224378 MS.gene073458:CDS 25.0%
! TATTCATTTCATGCATTAGA+GGG - chr2.1:55226732-55226751 MS.gene073458:intron 25.0%
! TCAATTAAAGGGAAAGTTTA+TGG - chr2.1:55226477-55226496 MS.gene073458:CDS 25.0%
! TCATAACATAACAATATTGG+AGG - chr2.1:55225683-55225702 MS.gene073458:intron 25.0%
! TGAAAATGACTAATCCTATT+AGG + chr2.1:55226711-55226730 None:intergenic 25.0%
! TTCCAATTGTGAAATTAGAT+GGG - chr2.1:55226547-55226566 MS.gene073458:intron 25.0%
! TTCTATGATGTACATATGTT+AGG - chr2.1:55225239-55225258 MS.gene073458:intron 25.0%
! TTGATGCATTTAATTCAACA+AGG + chr2.1:55225873-55225892 None:intergenic 25.0%
!! ATATTATTTTCCTTCTTCCT+AGG - chr2.1:55223581-55223600 MS.gene073458:CDS 25.0%
!! CACTAAAACATCAAAAGTTT+TGG - chr2.1:55225586-55225605 MS.gene073458:intron 25.0%
!! CATTTTTCTAACATTGTTTG+AGG - chr2.1:55223677-55223696 MS.gene073458:CDS 25.0%
!! TATTCAATGCAAGAACATTT+TGG + chr2.1:55224686-55224705 None:intergenic 25.0%
!! TTCATATCAAGTAGTTTTCT+TGG - chr2.1:55226117-55226136 MS.gene073458:intron 25.0%
!! TTTTCTTGAATGATTCTTGA+AGG + chr2.1:55224482-55224501 None:intergenic 25.0%
!!! ATCAGTAGATATTTTTGAAG+AGG - chr2.1:55225109-55225128 MS.gene073458:intron 25.0%
!!! ATGTTTTGGTATTTCCTAAT+AGG - chr2.1:55226694-55226713 MS.gene073458:intron 25.0%
!!! CTCTAAAACATCAAAAGAAA+AGG - chr2.1:55225622-55225641 MS.gene073458:intron 25.0%
!!! TTAACTCTTAACTTGTTTTC+TGG + chr2.1:55224349-55224368 None:intergenic 25.0%
!!! TTACGAATGGTTTTTTCTTA+GGG - chr2.1:55226433-55226452 MS.gene073458:CDS 25.0%
AAATGTTTCAGAAGCTCTTA+AGG + chr2.1:55225836-55225855 None:intergenic 30.0%
ATCAAAAGAAAAGGAATGGA+GGG - chr2.1:55225631-55225650 MS.gene073458:intron 30.0%
ATCAAAAGAAAAGGAATGGA+TGG + chr2.1:55225650-55225631 None:intergenic 30.0%
ATCAATCAAAGTAGTAAAGG+AGG + chr2.1:55224092-55224111 None:intergenic 30.0%
ATTAAAGTAGATGTGACCTT+TGG + chr2.1:55225515-55225534 None:intergenic 30.0%
ATTCACATGTGATAAACCAA+AGG - chr2.1:55225496-55225515 MS.gene073458:intron 30.0%
ATTTAGTGACATTGATCAAG+TGG - chr2.1:55226572-55226591 MS.gene073458:intron 30.0%
CATTGTTATAACAAAGCAAC+AGG - chr2.1:55226297-55226316 MS.gene073458:intron 30.0%
GAAGGAATTAAAGTTCAAAG+TGG - chr2.1:55226084-55226103 MS.gene073458:intron 30.0%
GTCAAATAATGTTGTAGTTG+TGG - chr2.1:55226788-55226807 MS.gene073458:intron 30.0%
GTTCCAATTGTGAAATTAGA+TGG - chr2.1:55226546-55226565 MS.gene073458:intron 30.0%
TACTTGATATGAAGGTTTCA+TGG + chr2.1:55226110-55226129 None:intergenic 30.0%
TCCAATTGTGAAATTAGATG+GGG - chr2.1:55226548-55226567 MS.gene073458:intron 30.0%
TCTCACCAAACAATACTAAA+GGG - chr2.1:55224813-55224832 MS.gene073458:intron 30.0%
TGCATCAATCAAAGTAGTAA+AGG + chr2.1:55224095-55224114 None:intergenic 30.0%
TGGAGTTATTAAGAAATTGC+AGG + chr2.1:55224751-55224770 None:intergenic 30.0%
TTGAGTGAGACTTAAATTGA+TGG + chr2.1:55224771-55224790 None:intergenic 30.0%
TTGTTCAAGTAATCTGTTGT+TGG + chr2.1:55225929-55225948 None:intergenic 30.0%
! AAAGGTGAAAGACAATTTTC+GGG - chr2.1:55226165-55226184 MS.gene073458:intron 30.0%
! ACTTTTACATCAATGACCAA+AGG - chr2.1:55225798-55225817 MS.gene073458:intron 30.0%
! AGAACTCGAAAAAAGTACAA+GGG - chr2.1:55224311-55224330 MS.gene073458:CDS 30.0%
! CAAAGGTGAAAGACAATTTT+CGG - chr2.1:55226164-55226183 MS.gene073458:intron 30.0%
! GAATTTTGTTGAAGTTAGCA+GGG - chr2.1:55226334-55226353 MS.gene073458:CDS 30.0%
! TATAACAAAGCAACAGGTTT+TGG - chr2.1:55226303-55226322 MS.gene073458:intron 30.0%
! TTTATGGTGTTTTCCAACAA+GGG - chr2.1:55225537-55225556 MS.gene073458:intron 30.0%
!! GATTTGTTCATTTTCCAACA+AGG + chr2.1:55224875-55224894 None:intergenic 30.0%
!! GGGTGATTTTTCAAATGTTT+TGG - chr2.1:55226680-55226699 MS.gene073458:intron 30.0%
!!! ACCTTTTTTTTGTGTGAAGA+TGG + chr2.1:55223535-55223554 None:intergenic 30.0%
!!! ATGATTTTAAGATCAACCGT+TGG - chr2.1:55226621-55226640 MS.gene073458:intron 30.0%
!!! GGTGTTATCATTGATTTTCA+AGG - chr2.1:55223972-55223991 MS.gene073458:CDS 30.0%
!!! GTTACGAATGGTTTTTTCTT+AGG - chr2.1:55226432-55226451 MS.gene073458:CDS 30.0%
!!! GTTATCATTGATTTTCAAGG+TGG - chr2.1:55223975-55223994 MS.gene073458:CDS 30.0%
!!! TACGAATGGTTTTTTCTTAG+GGG - chr2.1:55226434-55226453 MS.gene073458:CDS 30.0%
!!! TCTTTTGATGTTTTAGCTCT+TGG - chr2.1:55225398-55225417 MS.gene073458:intron 30.0%
!!! TGATTTTAAGATCAACCGTT+GGG - chr2.1:55226622-55226641 MS.gene073458:intron 30.0%
AAAGTGAGAAACCAGTTCAT+GGG - chr2.1:55226811-55226830 MS.gene073458:intron 35.0%
AAGTTGAATGTGGATGGTAA+TGG - chr2.1:55226645-55226664 MS.gene073458:intron 35.0%
AATCAATGATAACACCTCCA+AGG + chr2.1:55223968-55223987 None:intergenic 35.0%
ACAATCTCATTCTCTCTATG+TGG - chr2.1:55223779-55223798 MS.gene073458:CDS 35.0%
ACTGAATGTGCAACAAACTT+TGG + chr2.1:55226759-55226778 None:intergenic 35.0%
AGCTTCTACAGCTTTCATAA+TGG + chr2.1:55223895-55223914 None:intergenic 35.0%
CAAAGTAGTAAAGGAGGAAA+AGG + chr2.1:55224086-55224105 None:intergenic 35.0%
CATCAAAAGAAAAGGAATGG+AGG - chr2.1:55225630-55225649 MS.gene073458:intron 35.0%
CATCAATGACCAAAGGTAAA+TGG - chr2.1:55225805-55225824 MS.gene073458:intron 35.0%
CCAATTGTGAAATTAGATGG+GGG - chr2.1:55226549-55226568 MS.gene073458:intron 35.0%
CTCACCAAACAATACTAAAG+GGG - chr2.1:55224814-55224833 MS.gene073458:intron 35.0%
CTCTCACCAAACAATACTAA+AGG - chr2.1:55224812-55224831 MS.gene073458:intron 35.0%
GATCTGCACCATACTTAATT+GGG + chr2.1:55223845-55223864 None:intergenic 35.0%
GATTGGAAAATGAAGAAGAG+TGG - chr2.1:55223807-55223826 MS.gene073458:CDS 35.0%
GCACAAACCAGACAAAATAA+AGG - chr2.1:55225009-55225028 MS.gene073458:intron 35.0%
GCCATCTTCACACAAAAAAA+AGG - chr2.1:55223531-55223550 MS.gene073458:CDS 35.0%
GGATGGAGTAAGATGTTAAA+AGG + chr2.1:55225370-55225389 None:intergenic 35.0%
TCACCAAACAATACTAAAGG+GGG - chr2.1:55224815-55224834 MS.gene073458:intron 35.0%
TGCTGAGAAAATTGCAACAT+CGG + chr2.1:55226229-55226248 None:intergenic 35.0%
TTAGGGCTTGAATTATTACC+AGG - chr2.1:55223933-55223952 MS.gene073458:CDS 35.0%
TTCCAGCTACGAAAAAATTG+AGG + chr2.1:55224415-55224434 None:intergenic 35.0%
TTCGTAACATGAACTTGAAC+AGG + chr2.1:55226420-55226439 None:intergenic 35.0%
TTGAACTTTAATTCCTTCGG+TGG + chr2.1:55226082-55226101 None:intergenic 35.0%
TTTCTCAAGTTTACCCTTGT+TGG + chr2.1:55225553-55225572 None:intergenic 35.0%
! AGCCATAGGAATTGTTGTTA+GGG - chr2.1:55226248-55226267 MS.gene073458:intron 35.0%
! AGTGGGTTCAAAATTGTTAG+AGG + chr2.1:55224794-55224813 None:intergenic 35.0%
! ATGCTTTTGTTGCATTTTGG+AGG + chr2.1:55225906-55225925 None:intergenic 35.0%
! CAGAACTCGAAAAAAGTACA+AGG - chr2.1:55224310-55224329 MS.gene073458:CDS 35.0%
! GGAATTTTGTTGAAGTTAGC+AGG - chr2.1:55226333-55226352 MS.gene073458:CDS 35.0%
! GTTCAAGTTCATGTTACGAA+TGG - chr2.1:55226420-55226439 MS.gene073458:CDS 35.0%
! GTTTATGGTGTTTTCCAACA+AGG - chr2.1:55225536-55225555 MS.gene073458:intron 35.0%
! TAGTATTGTTTGGTGAGAGT+GGG + chr2.1:55224811-55224830 None:intergenic 35.0%
! TCATTCTCTCTATGTGGTTT+TGG - chr2.1:55223785-55223804 MS.gene073458:CDS 35.0%
! TGCTTTTGTTGCATTTTGGA+GGG + chr2.1:55225905-55225924 None:intergenic 35.0%
! TGGATGCTTTTGTTGCATTT+TGG + chr2.1:55225909-55225928 None:intergenic 35.0%
! TTAGTATTGTTTGGTGAGAG+TGG + chr2.1:55224812-55224831 None:intergenic 35.0%
! TTATCTGTTTTGCACTGCTA+AGG + chr2.1:55225180-55225199 None:intergenic 35.0%
! TTCATTTTCCAACAAGGCTT+TGG + chr2.1:55224869-55224888 None:intergenic 35.0%
!!! AACCTCAATTTTTTCGTAGC+TGG - chr2.1:55224410-55224429 MS.gene073458:CDS 35.0%
!!! AATGGTTTTTTCTTAGGGGA+TGG - chr2.1:55226438-55226457 MS.gene073458:CDS 35.0%
!!! ACTCTTAACTTGTTTTCTGG+TGG + chr2.1:55224346-55224365 None:intergenic 35.0%
!!! CAGATCTCCTTTATTTTGTC+TGG + chr2.1:55225019-55225038 None:intergenic 35.0%
!!! CTCTTAACTTGTTTTCTGGT+GGG + chr2.1:55224345-55224364 None:intergenic 35.0%
AAGGTTCCTCCATTTACCTT+TGG + chr2.1:55225817-55225836 None:intergenic 40.0%
AAGTGAGAAACCAGTTCATG+GGG - chr2.1:55226812-55226831 MS.gene073458:intron 40.0%
AATGAAGAAGAGTGGAAGAG+TGG - chr2.1:55223815-55223834 MS.gene073458:CDS 40.0%
ATCCACATTCAACTTCCCAA+CGG + chr2.1:55226640-55226659 None:intergenic 40.0%
CAATGACCAAAGGTAAATGG+AGG - chr2.1:55225808-55225827 MS.gene073458:intron 40.0%
CCCCCATCTAATTTCACAAT+TGG + chr2.1:55226552-55226571 None:intergenic 40.0%
GAAAGTGAGAAACCAGTTCA+TGG - chr2.1:55226810-55226829 MS.gene073458:intron 40.0%
GGATCTGCACCATACTTAAT+TGG + chr2.1:55223846-55223865 None:intergenic 40.0%
GTGGTGTACCCAATTAAGTA+TGG - chr2.1:55223834-55223853 MS.gene073458:CDS 40.0%
TAATTCCTTCGGTGGTGAAA+TGG + chr2.1:55226074-55226093 None:intergenic 40.0%
TAGCCCCCTTTAGTATTGTT+TGG + chr2.1:55224821-55224840 None:intergenic 40.0%
TCCCATCACATTTCCTTCAT+CGG - chr2.1:55224016-55224035 MS.gene073458:CDS 40.0%
TCCCCTAACAACAATTCCTA+TGG + chr2.1:55226253-55226272 None:intergenic 40.0%
TTACCAGGAATTAGAGACCT+TGG - chr2.1:55223948-55223967 MS.gene073458:CDS 40.0%
! AGCTCTTGGCACAAGTTTTA+AGG - chr2.1:55225412-55225431 MS.gene073458:intron 40.0%
! CAGCCATAGGAATTGTTGTT+AGG - chr2.1:55226247-55226266 MS.gene073458:intron 40.0%
! GCCATAGGAATTGTTGTTAG+GGG - chr2.1:55226249-55226268 MS.gene073458:intron 40.0%
! TCAACAAGGTGTCTATCAGT+TGG + chr2.1:55225859-55225878 None:intergenic 40.0%
! TGAGAAATCCAAAGCCTTGT+TGG - chr2.1:55224858-55224877 MS.gene073458:intron 40.0%
!! AACAAAGCAACAGGTTTTGG+TGG - chr2.1:55226306-55226325 MS.gene073458:intron 40.0%
!! ACAAGGCTTTGGATTTCTCA+AGG + chr2.1:55224858-55224877 None:intergenic 40.0%
!! ACCTCGAAGAGGTTGATATA+CGG - chr2.1:55224438-55224457 MS.gene073458:CDS 40.0%
!! GGATGGTAATGGCACAAAAT+GGG - chr2.1:55226656-55226675 MS.gene073458:intron 40.0%
!! TGGATGGTAATGGCACAAAA+TGG - chr2.1:55226655-55226674 MS.gene073458:intron 40.0%
!! TGTTGCATTTTGGAGGGTTT+TGG + chr2.1:55225899-55225918 None:intergenic 40.0%
!!! CTCTCTATGTGGTTTTGGAT+TGG - chr2.1:55223790-55223809 MS.gene073458:CDS 40.0%
AACCGTTGGGAAGTTGAATG+TGG - chr2.1:55226635-55226654 MS.gene073458:intron 45.0%
AAGGAAACAACCCCATGAAC+TGG + chr2.1:55226825-55226844 None:intergenic 45.0%
AAGTATGGTGCAGATCCAAC+AGG - chr2.1:55223849-55223868 MS.gene073458:CDS 45.0%
AGCCGATGAAGGAAATGTGA+TGG + chr2.1:55224021-55224040 None:intergenic 45.0%
AGTATGGTGCAGATCCAACA+GGG - chr2.1:55223850-55223869 MS.gene073458:CDS 45.0%
ATCACATTTCCTTCATCGGC+TGG - chr2.1:55224020-55224039 MS.gene073458:CDS 45.0%
CGATGAATATTCCTCCACCT+CGG + chr2.1:55226011-55226030 None:intergenic 45.0%
CTCGGTAACTTGAATCGAAG+AGG + chr2.1:55225993-55226012 None:intergenic 45.0%
CTTGGACAACACTCAACAGT+CGG - chr2.1:55226135-55226154 MS.gene073458:intron 45.0%
GCCGATGAAGGAAATGTGAT+GGG + chr2.1:55224020-55224039 None:intergenic 45.0%
GGACAAGCTAACATGATCAC+AGG - chr2.1:55226270-55226289 MS.gene073458:intron 45.0%
GTGTTTCCTAGTACGCTTCT+AGG + chr2.1:55224725-55224744 None:intergenic 45.0%
GTTGGGAAGTTGAATGTGGA+TGG - chr2.1:55226639-55226658 MS.gene073458:intron 45.0%
TCCGTATATCAACCTCTTCG+AGG + chr2.1:55224442-55224461 None:intergenic 45.0%
! CTCTTCGATTCAAGTTACCG+AGG - chr2.1:55225991-55226010 MS.gene073458:intron 45.0%
! CTGCTTTCATTTTCCCCTGT+TGG + chr2.1:55223867-55223886 None:intergenic 45.0%
! GCAATTTTCTCAGCAGCCAT+AGG - chr2.1:55226234-55226253 MS.gene073458:intron 45.0%
! GGTAATGGCACAAAATGGGT+GGG - chr2.1:55226660-55226679 MS.gene073458:intron 45.0%
! TTCGATTCAAGTTACCGAGG+TGG - chr2.1:55225994-55226013 MS.gene073458:intron 45.0%
!! GCAACAGGTTTTGGTGGAAT+TGG - chr2.1:55226312-55226331 MS.gene073458:intron 45.0%
!! TGGTAATGGCACAAAATGGG+TGG - chr2.1:55226659-55226678 MS.gene073458:intron 45.0%
!! AATTTAAATAAAACATTATT+TGG + chr2.1:55224517-55224536 None:intergenic 5.0%
ACAAGATTGCCAGCCGATGA+AGG + chr2.1:55224032-55224051 None:intergenic 50.0%
AGCTGGAAAACACCTCGAAG+AGG - chr2.1:55224427-55224446 MS.gene073458:CDS 50.0%
CCAGGAATTAGAGACCTTGG+AGG - chr2.1:55223951-55223970 MS.gene073458:CDS 50.0%
CCTAGTACGCTTCTAGGTAC+AGG + chr2.1:55224719-55224738 None:intergenic 50.0%
CCTCCAAGGTCTCTAATTCC+TGG + chr2.1:55223954-55223973 None:intergenic 50.0%
GTATGGTGCAGATCCAACAG+GGG - chr2.1:55223851-55223870 MS.gene073458:CDS 50.0%
GTGTGAAGATGGCTGAGACA+TGG + chr2.1:55223524-55223543 None:intergenic 50.0%
TTCCTCCATTTCACCACCGA+AGG - chr2.1:55226066-55226085 MS.gene073458:intron 50.0%
TTCCTTCGGTGGTGAAATGG+AGG + chr2.1:55226071-55226090 None:intergenic 50.0%
! CCTGTACCTAGAAGCGTACT+AGG - chr2.1:55224716-55224735 MS.gene073458:intron 50.0%
! GATTCAAGTTACCGAGGTGG+AGG - chr2.1:55225997-55226016 MS.gene073458:intron 50.0%
! TTCATCGGCTGGCAATCTTG+TGG - chr2.1:55224031-55224050 MS.gene073458:CDS 50.0%
GCTTCTAGGTACAGGCTCAC+TGG + chr2.1:55224711-55224730 None:intergenic 55.0%
GGACAACACTCAACAGTCGG+CGG - chr2.1:55226138-55226157 MS.gene073458:intron 55.0%
TCAACAGTCGGCGGAGACAA+AGG - chr2.1:55226147-55226166 MS.gene073458:intron 55.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 55223511 55226860 55223511 ID=MS.gene073458
chr2.1 mRNA 55223511 55226860 55223511 ID=MS.gene073458.t1;Parent=MS.gene073458
chr2.1 exon 55226819 55226860 55226819 ID=MS.gene073458.t1.exon1;Parent=MS.gene073458.t1
chr2.1 CDS 55226819 55226860 55226819 ID=cds.MS.gene073458.t1;Parent=MS.gene073458.t1
chr2.1 exon 55226319 55226546 55226319 ID=MS.gene073458.t1.exon2;Parent=MS.gene073458.t1
chr2.1 CDS 55226319 55226546 55226319 ID=cds.MS.gene073458.t1;Parent=MS.gene073458.t1
chr2.1 exon 55223511 55224551 55223511 ID=MS.gene073458.t1.exon3;Parent=MS.gene073458.t1
chr2.1 CDS 55223511 55224551 55223511 ID=cds.MS.gene073458.t1;Parent=MS.gene073458.t1
Gene Sequence

>MS.gene073458

ATGGACATTGCCATGTCTCAGCCATCTTCACACAAAAAAAAGGTATGTCACAAATTTATATAAATTTTTTATATTATTTTCCTTCTTCCTAGGAATTTTTTTTGATGATTTTAATTGGTATGCATCATGAGTATCTCACAATCAATCATAATTTTTTTTAGTTTTACATTTTTCTAACATTGTTTGAGGATGTAAACAATTTTACAATGACAATGTATCAAAATTACAATTACAATCTCCTTTTTTTCATTGTATAAAATGTAAAATTACAATCTCATTCTCTCTATGTGGTTTTGGATTGGAAAATGAAGAAGAGTGGAAGAGTGGTGTACCCAATTAAGTATGGTGCAGATCCAACAGGGGAAAATGAAAGCAGTGATTCCATTATGAAAGCTGTAGAAGCTGCATTTGATATTGATAATTTAGGGCTTGAATTATTACCAGGAATTAGAGACCTTGGAGGTGTTATCATTGATTTTCAAGGTGGAAACTATAAAATCAGCAATCCCATCACATTTCCTTCATCGGCTGGCAATCTTGTGGTAATCATCTTTCTCTTAGTTAATTTCTTTCCTTTTCCTCCTTTACTACTTTGATTGATGCAATTTTTTTTACTGCAAGATTAATTATGAACTTGTTGATCCTTAATTTGTTATATAAATTAGCATGTGTTTTTCTAAAATGATTTTTTTTAATATACTTGTAAAAAAATGACAGTTGTCGTTCTCAATAATGTGTTCATTTAACATCAAAATTTATTAAATGTAGTAAATGAGAGAAGTACAAAGAATACTTAACTCAGAACTCGAAAAAAGTACAAGGGATACTCGACCCACCAGAAAACAAGTTAAGAGTTAAAACAAAGAAGAGGGTTCAAAAACTAGTCAAAATACATGCAAAACCTCAATTTTTTCGTAGCTGGAAAACACCTCGAAGAGGTTGATATACGGAGTCTACTATCTCTTCTACCTTCAAGAATCATTCAAGAAAAAATAACCCCATTCCAAATAATGTTTTATTTAAATTATATATATTTTTTGATAATATTTATTATGAGGAATGTTTTTTTTTTTTGAAATTATGAACACTTCTTTTCAACGCTCTCTTTAATACTTGTTTCATTGTCTGTCCTATAAATAAATATTTTTCTCTTTTGATTTTTTTTATTGGCGCCAAAATGTTCTTGCATTGAATACTCCAGTGAGCCTGTACCTAGAAGCGTACTAGGAAACACTATCCTGCAATTTCTTAATAACTCCATCAATTTAAGTCTCACTCAACCTCTAACAATTTTGAACCCACTCTCACCAAACAATACTAAAGGGGGCTAACAAGATATGAATACCTTGAGAAATCCAAAGCCTTGTTGGAAAATGAACAAATCTATCGATTCAATAGAATAAAAAAGGTAAAAGTAAACAATAAAAAATGAGATTTTAAAAAAATTATGGAGTGACAAGACTAGATACGAGAGTATAAATAAAATCTTTCAAAAGATGCACAAACCAGACAAAATAAAGGAGATCTGTGTTATCAATTATAATTCAAATTTAATTTGTAAACATGGATAGTGTAAATCTATTTTGCACATTCATCTAATCAGTAGATATTTTTGAAGAGGAATTTTTTTTTTTTGAATAAGGAATATATTATTAATATCAATATTCCTTAGCAGTGCAAAACAGATAAAAATGTGTAATATAGAATTTTTCTATGATGTGCAAATTTTTCTATGATGTACATATGTTAGGAAGTTCAAAATAATAAATTTGTATTGGAACTTGTGCATTTTATATTAAATATTATTTTTTAATAAATAATTGTTGCCTTTCAATAAAATTTTGCAACTTTTAGTTCCTTTTAACATCTTACTCCATCCATTCCTTTTCTTTTGATGTTTTAGCTCTTGGCACAAGTTTTAAGGAGTAATTAAAGTATATAAATATTTATGTGTTTTTGACTAAAATGTCCTTATTTATCATTAAATTCACATGTGATAAACCAAAGGTCACATCTACTTTAATAGTTTATGGTGTTTTCCAACAAGGGTAAACTTGAGAAAACAATAATAAATTCACTAAAACATCAAAAGTTTTGGAATATAATTGAAACTCTAAAACATCAAAAGAAAAGGAATGGAGGGAGTAATATGCTTCTTGATAATTGTATAAATCATAACATAACAATATTGGAGGGAAGTTAAATTGCAGTCTTTTTTCTTGAAAAATGCTGCAGTGCTATTTTCTTCTTCCACTTAACTTTTACATATTATTATTTCTTACACTTAACTTTTACATCAATGACCAAAGGTAAATGGAGGAACCTTAAGAGCTTCTGAAACATTTCCAACTGATAGACACCTTGTTGAATTAAATGCATCAAATTCCAAAACCCTCCAAAATGCAACAAAAGCATCCAACAACAGATTACTTGAACAAACAATAGGAATTTACTATGAAGACATAACATTTAGAGACATCCTCTTCGATTCAAGTTACCGAGGTGGAGGAATATTCATCGTCGATTCAGCTAGAATCAGAATCGACAATTGTTTCTTCCTCCATTTCACCACCGAAGGAATTAAAGTTCAAAGTGGCCATGAAACCTTCATATCAAGTAGTTTTCTTGGACAACACTCAACAGTCGGCGGAGACAAAGGTGAAAGACAATTTTCGGGAACTGCAATTGATCTTGCTAGTAATGACAATGCTATCACCGATGTTGCAATTTTCTCAGCAGCCATAGGAATTGTTGTTAGGGGACAAGCTAACATGATCACAGGTGTGCATTGTTATAACAAAGCAACAGGTTTTGGTGGAATTGGAATTTTGTTGAAGTTAGCAGGGAATTCACAAACAAGAATTGATAATTGTTACATGGATTATAATTCTATAGTCATGGAAGATCCTGTTCAAGTTCATGTTACGAATGGTTTTTTCTTAGGGGATGGTAATATTGTTTTGAAATCAATTAAAGGGAAAGTTTATGGCTTAAATATTGTTAACAATATGTTTAGTGGAAACCCTAATAATAATGTTCCAATTGTGAAATTAGATGGGGGATTTAGTGACATTGATCAAGTGGTGATTGATATGAACAATGTGATTGGTATGATTTTAAGATCAACCGTTGGGAAGTTGAATGTGGATGGTAATGGCACAAAATGGGTGGGTGATTTTTCAAATGTTTTGGTATTTCCTAATAGGATTAGTCATTTTCAATATTCATTTCATGCATTAGAGGGACCAAAGTTTGTTGCACATTCAGTGAGTAATGTGTCAAATAATGTTGTAGTTGTGGAAAGTGAGAAACCAGTTCATGGGGTTGTTTCCTTCTTTGTTGAACAATGA
Protein sequence

>MS.gene073458.t1

MDIAMSQPSSHKKKSGRVVYPIKYGADPTGENESSDSIMKAVEAAFDIDNLGLELLPGIRDLGGVIIDFQGGNYKISNPITFPSSAGNLVVNGGTLRASETFPTDRHLVELNASNSKTLQNATKASNNRLLEQTIGIYYEDITFRDILFDSSYRGGGIFIVDSARIRIDNCFFLHFTTEGIKVQSGHETFISSSFLGQHSTVGGDKGERQFSGTAIDLASNDNAITDVAIFSAAIGIVVRGQANMITGVHCYNKATGFGGIGILLKLAGNSQTRIDNCYMDYNSIVMEDPVQVHVTNGFFLGDGNIVLKSIKGKVYGLNIVNNMFSGNPNNNVPIVKLDGGFSDIDQVVIDMNNVIGMILRSTVGKLNVDGNGTKWVGDFSNVLVFPNRISHFQYSFHALEGPKFVAHSVSNVSNNVVVVESEKPVHGVVSFFVEQ