AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene073461

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073461.t1 MTR_2g035820 99.576 236 1 0 1 236 1 236 2.79e-177 486
MS.gene073461.t1 MTR_0003s0620 51.748 143 68 1 2 144 7 148 4.19e-48 155
MS.gene073461.t1 MTR_0003s0620 36.638 232 103 3 2 233 7 194 1.35e-42 143
MS.gene073461.t1 MTR_8g085220 35.526 152 88 5 14 157 25 174 1.26e-20 90.5
MS.gene073461.t1 MTR_8g085220 35.526 152 88 5 14 157 25 174 1.35e-20 90.5
MS.gene073461.t1 MTR_4g068950 27.174 184 120 6 3 175 15 195 2.98e-19 84.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073461.t1 AT3G17365 69.038 239 70 3 1 236 1 238 1.16e-122 348
MS.gene073461.t1 AT3G17365 55.235 277 82 6 1 236 1 276 9.18e-101 294
MS.gene073461.t1 AT3G60910 41.921 229 129 3 2 227 7 234 3.87e-63 197
MS.gene073461.t1 AT3G60910 42.197 173 96 3 58 227 1 172 6.85e-44 146
MS.gene073461.t1 AT4G34360 29.444 180 113 7 7 175 14 190 4.98e-20 85.9
MS.gene073461.t1 AT2G31740 31.551 187 113 7 14 188 29 212 2.90e-17 80.9

Find 51 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAAGATGCTTGAGGAAATT+TGG 0.165677 2.1:+55203244 MS.gene073461:CDS
TCTATCCTTGTTGTTGGTTC+TGG 0.233828 2.1:+55202093 MS.gene073461:CDS
GTTGTTGGTTCTGGTAATTC+AGG 0.253017 2.1:+55202102 MS.gene073461:CDS
TAGTTAGACAGATTTGAAAA+TGG 0.318761 2.1:+55202928 MS.gene073461:intron
CCATCATTATTAAGTGGAAT+AGG 0.346132 2.1:-55203906 None:intergenic
ACAATTAAACTCCATGTAAT+AGG 0.352596 2.1:+55203580 MS.gene073461:CDS
GAGCCAAAGTAATATACTTT+TGG 0.356009 2.1:-55202038 None:intergenic
TAGTGCAAATGTATATATAA+TGG 0.365312 2.1:-55203968 None:intergenic
AGGAACTCTTGATTCTATCT+TGG 0.371492 2.1:+55203114 MS.gene073461:intron
AACAAGGATAGATTGGTTCT+TGG 0.386383 2.1:-55202082 None:intergenic
AACCAATCTATCCTTGTTGT+TGG 0.393670 2.1:+55202087 MS.gene073461:CDS
AAAATCTGATAATCCACTTT+GGG 0.403584 2.1:+55203872 MS.gene073461:CDS
GGACATCGGGATTCAGTCCT+AGG 0.416500 2.1:-55203947 None:intergenic
ACAAGGATAGATTGGTTCTT+GGG 0.427283 2.1:-55202081 None:intergenic
GTACCAAAAGTATATTACTT+TGG 0.445138 2.1:+55202035 MS.gene073461:CDS
GGCTAAAGGAGTCTATATTC+TGG 0.446501 2.1:+55203394 MS.gene073461:CDS
CCTATTCCACTTAATAATGA+TGG 0.448893 2.1:+55203906 MS.gene073461:CDS
AACCAACAACAAGGATAGAT+TGG 0.452329 2.1:-55202089 None:intergenic
GAGCTTCCATCATTATTAAG+TGG 0.453928 2.1:-55203912 None:intergenic
CATATGGTGAATCATGGTAC+TGG 0.459818 2.1:+55201974 MS.gene073461:CDS
GAACAAACAGTGATGGCAAC+AGG 0.463928 2.1:+55201943 None:intergenic
GGAACATAGAGATTGATGAT+AGG 0.472893 2.1:-55202060 None:intergenic
AGCAAACGTAAGCGATATAG+TGG 0.489409 2.1:-55203538 None:intergenic
ATGTATATATAATGGACATC+GGG 0.504347 2.1:-55203960 None:intergenic
ATTTAAACCAACCTATTACA+TGG 0.504757 2.1:-55203591 None:intergenic
TTATATATACATTTGCACTA+AGG 0.515855 2.1:+55203971 MS.gene073461:CDS
TATAGGGTTCTCAAGGCTAA+AGG 0.523386 2.1:+55203380 MS.gene073461:intron
GCAACAGGTACACAAGCATA+TGG 0.530042 2.1:+55201958 MS.gene073461:CDS
GACCTGGTTCGTTTGTGTAA+CGG 0.530093 2.1:-55202002 None:intergenic
TTTGGTTCTGTTATTGACAA+AGG 0.530391 2.1:+55202986 MS.gene073461:CDS
CACAAGCATATGGTGAATCA+TGG 0.535442 2.1:+55201968 MS.gene073461:CDS
TGCAGCGTTCAGTCAAGGCA+TGG 0.540936 2.1:+55202328 MS.gene073461:intron
TTGTTTGCAGCGTTCAGTCA+AGG 0.543732 2.1:+55202323 MS.gene073461:intron
GAACATTGATATTTCTTCAG+TGG 0.546761 2.1:+55202379 MS.gene073461:CDS
AGTCAAGGCATGGTTGATGA+AGG 0.554093 2.1:+55202338 MS.gene073461:CDS
TGGTACCAATCAAAAGGACC+TGG 0.568031 2.1:-55202018 None:intergenic
AACCGTTACACAAACGAACC+AGG 0.571683 2.1:+55202000 MS.gene073461:CDS
ATAAGGATACACTTCAATTG+AGG 0.578933 2.1:-55202437 None:intergenic
AGATGCTTGAGGAAATTTGG+AGG 0.579739 2.1:+55203247 MS.gene073461:CDS
GGTTGATGAAGGTGGTTACA+AGG 0.580058 2.1:+55202349 MS.gene073461:CDS
GACACACTATAGGGTTCTCA+AGG 0.580644 2.1:+55203373 MS.gene073461:intron
AATGTATATATAATGGACAT+CGG 0.584034 2.1:-55203961 None:intergenic
ACTTCAATTGAGGACGATCT+CGG 0.589584 2.1:-55202427 None:intergenic
TGCTACGTGATTCATGCTCG+TGG 0.598791 2.1:+55203557 MS.gene073461:CDS
GACATCGGGATTCAGTCCTA+GGG 0.605591 2.1:-55203946 None:intergenic
CAAGGCATGGTTGATGAAGG+TGG 0.620512 2.1:+55202341 MS.gene073461:CDS
ATATGGTGAATCATGGTACT+GGG 0.622071 2.1:+55201975 MS.gene073461:CDS
ACATGCTACTAAGATGCTTG+AGG 0.629503 2.1:+55203236 MS.gene073461:CDS
AGCTCTGTGGATGAAACCCT+AGG 0.652280 2.1:+55203930 MS.gene073461:CDS
TAATAATGATGGAAGCTCTG+TGG 0.664960 2.1:+55203917 MS.gene073461:CDS
AATTACCAGAACCAACAACA+AGG 0.677098 2.1:-55202098 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACAATATATTTATACAA+AGG - chr2.1:55202762-55202781 None:intergenic 10.0%
!! ATCAACAATATTAATAAAAT+GGG + chr2.1:55204972-55204991 MS.gene073461:intron 10.0%
!! ATGTATAAAAATATTTATTG+CGG + chr2.1:55202724-55202743 MS.gene073461:intron 10.0%
!! TATCAACAATATTAATAAAA+TGG + chr2.1:55204971-55204990 MS.gene073461:intron 10.0%
!!! AAAAAAAATGTTTTGTTTTT+TGG - chr2.1:55202137-55202156 None:intergenic 10.0%
!!! AAATGTTTTTTTTAGAAAAA+TGG - chr2.1:55203691-55203710 None:intergenic 10.0%
!! AACTTCATAATTTAAAAACA+TGG - chr2.1:55202485-55202504 None:intergenic 15.0%
!! ATAAATATGATTCTCATATA+TGG + chr2.1:55204386-55204405 MS.gene073461:intron 15.0%
!! ATTATATTTATAGAGAATTG+TGG + chr2.1:55202527-55202546 MS.gene073461:intron 15.0%
!! TCAACAATATTAATAAAATG+GGG + chr2.1:55204973-55204992 MS.gene073461:intron 15.0%
!!! ATGTTTTTAAATTATGAAGT+TGG + chr2.1:55202484-55202503 MS.gene073461:intron 15.0%
!!! ATTGTTTAATTTTGTTTTTC+AGG + chr2.1:55203094-55203113 MS.gene073461:intron 15.0%
!!! TTTTATTATTCTACATTGAA+GGG - chr2.1:55204193-55204212 None:intergenic 15.0%
!!! TTTTTATTATTCTACATTGA+AGG - chr2.1:55204194-55204213 None:intergenic 15.0%
!! AAATGAATCTATGAATGAAT+GGG - chr2.1:55203019-55203038 None:intergenic 20.0%
!! AACATGAATTAATCAACATA+AGG - chr2.1:55202457-55202476 None:intergenic 20.0%
!! ATTATAACATGGATATAACA+TGG - chr2.1:55202843-55202862 None:intergenic 20.0%
!! ATTTGAATAATTAATCGTCA+TGG + chr2.1:55205801-55205820 MS.gene073461:intron 20.0%
!! CAAATGAAAAAGAATAAATG+GGG - chr2.1:55202199-55202218 None:intergenic 20.0%
!! CAACAATATTAATAAAATGG+GGG + chr2.1:55204974-55204993 MS.gene073461:intron 20.0%
!! CCAAATGAAAAAGAATAAAT+GGG - chr2.1:55202200-55202219 None:intergenic 20.0%
!! CGATTAATTATTCAAATTAG+CGG - chr2.1:55205798-55205817 None:intergenic 20.0%
!! TAAATGAATCTATGAATGAA+TGG - chr2.1:55203020-55203039 None:intergenic 20.0%
!! TAGTGCAAATGTATATATAA+TGG - chr2.1:55203971-55203990 None:intergenic 20.0%
!! TTATAAAGGAACACATAAAT+AGG - chr2.1:55203727-55203746 None:intergenic 20.0%
!! TTATATATACATTTGCACTA+AGG + chr2.1:55203971-55203990 MS.gene073461:CDS 20.0%
!! TTTAATTAGTGTCATGTAAA+TGG + chr2.1:55204726-55204745 MS.gene073461:intron 20.0%
!!! AAAAAATTTAGTTTCCTAAC+AGG + chr2.1:55204489-55204508 MS.gene073461:intron 20.0%
!!! AATGTATATATAATGGACAT+CGG - chr2.1:55203964-55203983 None:intergenic 20.0%
!!! AATTTTGTTGGCTATTTTAT+AGG - chr2.1:55204549-55204568 None:intergenic 20.0%
!!! AGAATTTCTTTTATAAAGAG+AGG - chr2.1:55205476-55205495 None:intergenic 20.0%
!!! CCATTTATTCTTTTTCATTT+GGG + chr2.1:55202198-55202217 MS.gene073461:intron 20.0%
!!! GAATTTCTTTTATAAAGAGA+GGG - chr2.1:55205475-55205494 None:intergenic 20.0%
!!! TTTATAAAATTTTGCTGAAG+TGG + chr2.1:55202863-55202882 MS.gene073461:intron 20.0%
!!! TTTATACATGTTTAGTTTGA+TGG - chr2.1:55202713-55202732 None:intergenic 20.0%
! AAAAATCTGATAATCCACTT+TGG + chr2.1:55203871-55203890 MS.gene073461:CDS 25.0%
! AAAATCTGATAATCCACTTT+GGG + chr2.1:55203872-55203891 MS.gene073461:CDS 25.0%
! AAAATCTTCTTCACTCTAAA+GGG - chr2.1:55205258-55205277 None:intergenic 25.0%
! AATATTAATAAAATGGGGGA+AGG + chr2.1:55204978-55204997 MS.gene073461:intron 25.0%
! ACAATTAAACTCCATGTAAT+AGG + chr2.1:55203580-55203599 MS.gene073461:CDS 25.0%
! AGAAGAAACACAGTAATAAA+GGG - chr2.1:55205064-55205083 None:intergenic 25.0%
! ATGGATATAACATGGAAAAT+TGG - chr2.1:55202835-55202854 None:intergenic 25.0%
! ATGTGATTTATTTACTACGA+TGG + chr2.1:55205121-55205140 MS.gene073461:intron 25.0%
! ATTTAAACCAACCTATTACA+TGG - chr2.1:55203594-55203613 None:intergenic 25.0%
! CAAAAAATGACAATGATAAG+AGG - chr2.1:55203617-55203636 None:intergenic 25.0%
! CCCAAATGAAAAAGAATAAA+TGG - chr2.1:55202201-55202220 None:intergenic 25.0%
! GAATAAATCTGAATACAACT+CGG - chr2.1:55203438-55203457 None:intergenic 25.0%
! TAGTTAGACAGATTTGAAAA+TGG + chr2.1:55202928-55202947 MS.gene073461:intron 25.0%
! TCAAATCTGTCTAACTATTA+AGG - chr2.1:55202925-55202944 None:intergenic 25.0%
! TCATCTTGTTGAAAATACTT+AGG + chr2.1:55205659-55205678 MS.gene073461:intron 25.0%
! TTAGACAACTCTCAAAAAAA+AGG - chr2.1:55204074-55204093 None:intergenic 25.0%
! TTCAACTAGATTATCAACAA+TGG - chr2.1:55204946-55204965 None:intergenic 25.0%
!! GTACCAAAAGTATATTACTT+TGG + chr2.1:55202035-55202054 MS.gene073461:CDS 25.0%
!! TACTTACTCTTTTATGTTCT+TGG + chr2.1:55204012-55204031 MS.gene073461:intron 25.0%
!! TCATAAATTTTACCAAACGA+AGG + chr2.1:55204094-55204113 MS.gene073461:intron 25.0%
!!! ACAGAATGATTAGTCTAAAT+AGG - chr2.1:55205879-55205898 None:intergenic 25.0%
!!! ATATTTTTGTTGTACTAGTG+TGG + chr2.1:55203198-55203217 MS.gene073461:intron 25.0%
!!! ATGTATATATAATGGACATC+GGG - chr2.1:55203963-55203982 None:intergenic 25.0%
!!! CCCATTTATTCTTTTTCATT+TGG + chr2.1:55202197-55202216 MS.gene073461:intron 25.0%
AAGACATCAAAGTGTATGTT+TGG + chr2.1:55202577-55202596 MS.gene073461:intron 30.0%
AATCCAAAATGAAACAGAAG+TGG - chr2.1:55203311-55203330 None:intergenic 30.0%
ACATAAATAGGATGCTTCAA+AGG - chr2.1:55203715-55203734 None:intergenic 30.0%
ACTAATGCACAATTTCACTT+CGG - chr2.1:55205307-55205326 None:intergenic 30.0%
ATAAGGATACACTTCAATTG+AGG - chr2.1:55202440-55202459 None:intergenic 30.0%
ATTATTCAAATTAGCGGCAA+TGG - chr2.1:55205792-55205811 None:intergenic 30.0%
ATTGCATGTTAAGCTTAGAA+GGG - chr2.1:55204917-55204936 None:intergenic 30.0%
ATTGCTATGATATCAAGGTT+TGG - chr2.1:55204318-55204337 None:intergenic 30.0%
ATTTGCGGATTTCCTATAAT+AGG + chr2.1:55204573-55204592 MS.gene073461:intron 30.0%
CAAAATCTTCTTCACTCTAA+AGG - chr2.1:55205259-55205278 None:intergenic 30.0%
CATAAATAGGATGCTTCAAA+GGG - chr2.1:55203714-55203733 None:intergenic 30.0%
CCAACAAAATTATGCATTTG+CGG + chr2.1:55204558-55204577 MS.gene073461:intron 30.0%
CCATCATTATTAAGTGGAAT+AGG - chr2.1:55203909-55203928 None:intergenic 30.0%
CCTATTCCACTTAATAATGA+TGG + chr2.1:55203906-55203925 MS.gene073461:CDS 30.0%
GAACATTGATATTTCTTCAG+TGG + chr2.1:55202379-55202398 MS.gene073461:CDS 30.0%
GAGAAGAAACACAGTAATAA+AGG - chr2.1:55205065-55205084 None:intergenic 30.0%
GCTTGTTCTTATAGAATGAT+TGG - chr2.1:55204801-55204820 None:intergenic 30.0%
GTAGAATATAATGTACCTCT+AGG + chr2.1:55204748-55204767 MS.gene073461:intron 30.0%
TCTGTTATAATCTTTCTGAC+AGG + chr2.1:55205894-55205913 MS.gene073461:intron 30.0%
TGATTTATTTACTACGATGG+TGG + chr2.1:55205124-55205143 MS.gene073461:intron 30.0%
TTAAACTCCATGTAATAGGT+TGG + chr2.1:55203584-55203603 MS.gene073461:intron 30.0%
TTAGTCCCTAAAAGCAAATT+TGG + chr2.1:55204840-55204859 MS.gene073461:intron 30.0%
TTATTCAAATTAGCGGCAAT+GGG - chr2.1:55205791-55205810 None:intergenic 30.0%
TTGCATGAATTAGAAAGTCA+TGG - chr2.1:55204625-55204644 None:intergenic 30.0%
TTTATAAATTTGCGCCTCTT+CGG + chr2.1:55203740-55203759 MS.gene073461:intron 30.0%
TTTGCGGATTTCCTATAATA+GGG + chr2.1:55204574-55204593 MS.gene073461:intron 30.0%
! AACTATGGACTTTTTGTTCA+TGG - chr2.1:55203283-55203302 None:intergenic 30.0%
! GAGCCAAAGTAATATACTTT+TGG - chr2.1:55202041-55202060 None:intergenic 30.0%
! GATTTTCCACTTCAAATTTG+CGG - chr2.1:55204171-55204190 None:intergenic 30.0%
! TACTTTTGGTACCAATCAAA+AGG - chr2.1:55202027-55202046 None:intergenic 30.0%
! TAGTATCTCTAGTGTGATTT+TGG + chr2.1:55202628-55202647 MS.gene073461:intron 30.0%
! TCTTTACAATGGTGGAAATA+TGG + chr2.1:55205354-55205373 MS.gene073461:intron 30.0%
! TTACCACTTCTGTTTCATTT+TGG + chr2.1:55203305-55203324 MS.gene073461:intron 30.0%
! TTCTTTTATAAAGAGAGGGA+AGG - chr2.1:55205471-55205490 None:intergenic 30.0%
!! ATCCAACCAAATTTGCTTTT+AGG - chr2.1:55204849-55204868 None:intergenic 30.0%
!! TCCAACCAAATTTGCTTTTA+GGG - chr2.1:55204848-55204867 None:intergenic 30.0%
!! TGATTAGTCTAAATAGGCAA+AGG - chr2.1:55205873-55205892 None:intergenic 30.0%
!! TTTCTTCTCCTTTTGAAAGT+TGG + chr2.1:55205076-55205095 MS.gene073461:intron 30.0%
!! TTTGGTTCTGTTATTGACAA+AGG + chr2.1:55202986-55203005 MS.gene073461:CDS 30.0%
!!! TTCGGTTTTCTAGTTTCTAT+CGG - chr2.1:55205289-55205308 None:intergenic 30.0%
AACCAACAACAAGGATAGAT+TGG - chr2.1:55202092-55202111 None:intergenic 35.0%
AAGAAATTCTCCTAGACTTC+AGG + chr2.1:55205486-55205505 MS.gene073461:intron 35.0%
AAGAAGATTGAAGAAGCTGA+AGG - chr2.1:55205534-55205553 None:intergenic 35.0%
AAGATAAGCATAACGAAGAG+TGG - chr2.1:55204419-55204438 None:intergenic 35.0%
AAGTTTCTCTGCACATATCA+CGG - chr2.1:55203841-55203860 None:intergenic 35.0%
AATGATTGGAAACTCACGAA+TGG - chr2.1:55204787-55204806 None:intergenic 35.0%
AATTACCAGAACCAACAACA+AGG - chr2.1:55202101-55202120 None:intergenic 35.0%
ACAGAAGTGGTAAAGAACTA+TGG - chr2.1:55203298-55203317 None:intergenic 35.0%
ACTCCAGATATGAATGAACT+AGG - chr2.1:55203789-55203808 None:intergenic 35.0%
AGAAATTCTCCTAGACTTCA+GGG + chr2.1:55205487-55205506 MS.gene073461:intron 35.0%
AGGAACTCTTGATTCTATCT+TGG + chr2.1:55203114-55203133 MS.gene073461:intron 35.0%
ATATGGTGAATCATGGTACT+GGG + chr2.1:55201975-55201994 MS.gene073461:CDS 35.0%
ATGTGGGCATGTAAATTTCA+AGG + chr2.1:55202277-55202296 MS.gene073461:intron 35.0%
ATGTTCTTGGTCAATAGTGT+TGG + chr2.1:55204025-55204044 MS.gene073461:intron 35.0%
CATTGCATGTTAAGCTTAGA+AGG - chr2.1:55204918-55204937 None:intergenic 35.0%
CTAAGATGCTTGAGGAAATT+TGG + chr2.1:55203244-55203263 MS.gene073461:CDS 35.0%
CTAAGCTTAACATGCAATGA+AGG + chr2.1:55204919-55204938 MS.gene073461:intron 35.0%
CTGAAGGAATAAAGGTTGTT+AGG - chr2.1:55205518-55205537 None:intergenic 35.0%
GAACAGTTCATGTGTTAACT+TGG - chr2.1:55203642-55203661 None:intergenic 35.0%
GAGCTTCCATCATTATTAAG+TGG - chr2.1:55203915-55203934 None:intergenic 35.0%
GATTCAGTGAAATTGCCTTA+GGG - chr2.1:55204366-55204385 None:intergenic 35.0%
GCACAATTGCTATGATATCA+AGG - chr2.1:55204323-55204342 None:intergenic 35.0%
GGGAACAAATGAAAACTGAA+TGG - chr2.1:55205771-55205790 None:intergenic 35.0%
GTCATCAACTGCTTCAATAA+TGG - chr2.1:55203356-55203375 None:intergenic 35.0%
GTGGAAGGAATCAACTAATA+AGG - chr2.1:55204676-55204695 None:intergenic 35.0%
TAGAGAATTGTGGTCAAATG+CGG + chr2.1:55202537-55202556 MS.gene073461:intron 35.0%
TCCCTAAAAGCAAATTTGGT+TGG + chr2.1:55204844-55204863 MS.gene073461:intron 35.0%
TGAAGAAGCTGAAGGAATAA+AGG - chr2.1:55205526-55205545 None:intergenic 35.0%
TGAAGGAGAGTCAAAACATT+TGG - chr2.1:55204454-55204473 None:intergenic 35.0%
TGAGATGTGTTCCCTATTAT+AGG - chr2.1:55204588-55204607 None:intergenic 35.0%
TGATTCAGTGAAATTGCCTT+AGG - chr2.1:55204367-55204386 None:intergenic 35.0%
TGCATTAGTGACTCTGATAA+TGG + chr2.1:55205318-55205337 MS.gene073461:intron 35.0%
TTGCCTAGTTCATTCATATC+TGG + chr2.1:55203783-55203802 MS.gene073461:intron 35.0%
TTGTTAAAGTTGCCTTCGTT+TGG - chr2.1:55204109-55204128 None:intergenic 35.0%
! AACAAGGATAGATTGGTTCT+TGG - chr2.1:55202085-55202104 None:intergenic 35.0%
! AACCAATCTATCCTTGTTGT+TGG + chr2.1:55202087-55202106 MS.gene073461:CDS 35.0%
! AAGACTAACAGTTTGCTCAT+TGG + chr2.1:55203175-55203194 MS.gene073461:intron 35.0%
! AAGTACTGCCAACTTTCAAA+AGG - chr2.1:55205087-55205106 None:intergenic 35.0%
! ACAAGGATAGATTGGTTCTT+GGG - chr2.1:55202084-55202103 None:intergenic 35.0%
! ACCTTTTGTGTTATGCATGT+GGG + chr2.1:55202261-55202280 MS.gene073461:intron 35.0%
! CCGCAAATGCATAATTTTGT+TGG - chr2.1:55204561-55204580 None:intergenic 35.0%
! GAAGAGGCGCAAATTTATAA+AGG - chr2.1:55203741-55203760 None:intergenic 35.0%
! GGAACATAGAGATTGATGAT+AGG - chr2.1:55202063-55202082 None:intergenic 35.0%
! TAATAATGATGGAAGCTCTG+TGG + chr2.1:55203917-55203936 MS.gene073461:CDS 35.0%
! TTCTTCACTCTAAAGGGTAT+TGG - chr2.1:55205252-55205271 None:intergenic 35.0%
!! GTTTAGTTTGATGGTGAGTT+TGG - chr2.1:55202704-55202723 None:intergenic 35.0%
!! TTTGACTCGATGCATTTTGA+AGG - chr2.1:55204471-55204490 None:intergenic 35.0%
!!! TTTTTTGAAGATAGCCAGTG+AGG + chr2.1:55204225-55204244 MS.gene073461:intron 35.0%
ACATGCTACTAAGATGCTTG+AGG + chr2.1:55203236-55203255 MS.gene073461:CDS 40.0%
ACTTCAATTGAGGACGATCT+CGG - chr2.1:55202430-55202449 None:intergenic 40.0%
AGATGCTTGAGGAAATTTGG+AGG + chr2.1:55203247-55203266 MS.gene073461:CDS 40.0%
AGATTGCCGCAAATTTGAAG+TGG + chr2.1:55204162-55204181 MS.gene073461:intron 40.0%
AGCAAACGTAAGCGATATAG+TGG - chr2.1:55203541-55203560 None:intergenic 40.0%
CAAAAATTGCAGGTGACGTA+TGG + chr2.1:55203511-55203530 MS.gene073461:intron 40.0%
CACAAGCATATGGTGAATCA+TGG + chr2.1:55201968-55201987 MS.gene073461:CDS 40.0%
CATATGGTGAATCATGGTAC+TGG + chr2.1:55201974-55201993 MS.gene073461:CDS 40.0%
CTTGAAGCTCTTTACAATGG+TGG + chr2.1:55205346-55205365 MS.gene073461:intron 40.0%
GAAACTGCACTAGTGTGAAT+TGG + chr2.1:55205554-55205573 MS.gene073461:intron 40.0%
GAATACAACTCGGTACTAAG+AGG - chr2.1:55203428-55203447 None:intergenic 40.0%
GAGCTTGAAGCTCTTTACAA+TGG + chr2.1:55205343-55205362 MS.gene073461:intron 40.0%
GCCTATATATTTGACCGAAG+AGG - chr2.1:55203757-55203776 None:intergenic 40.0%
GCCTCTTCGGTCAAATATAT+AGG + chr2.1:55203753-55203772 MS.gene073461:intron 40.0%
GGCTAAAGGAGTCTATATTC+TGG + chr2.1:55203394-55203413 MS.gene073461:CDS 40.0%
GGTTCTACATATGTTCCTAG+AGG - chr2.1:55204766-55204785 None:intergenic 40.0%
GTCTCAAAATTCTCTCCCTA+AGG + chr2.1:55204348-55204367 MS.gene073461:intron 40.0%
TAAGCATAACGAAGAGTGGA+CGG - chr2.1:55204415-55204434 None:intergenic 40.0%
TAGGATATACCCTGAAGTCT+AGG - chr2.1:55205499-55205518 None:intergenic 40.0%
TATAGGGTTCTCAAGGCTAA+AGG + chr2.1:55203380-55203399 MS.gene073461:intron 40.0%
TCAAGTAAGTCCTCCTAAGT+AGG - chr2.1:55204266-55204285 None:intergenic 40.0%
TGAATGCGTGCAAAAATTGC+AGG + chr2.1:55203501-55203520 MS.gene073461:intron 40.0%
! AGGAAACTGATAGAGCATCA+GGG - chr2.1:55205611-55205630 None:intergenic 40.0%
! CACCTTTTGTGTTATGCATG+TGG + chr2.1:55202260-55202279 MS.gene073461:intron 40.0%
! GCTTTTGCATCAGAGACTTT+TGG + chr2.1:55202968-55202987 MS.gene073461:CDS 40.0%
! GGATTCACAATGATTTTGCC+AGG + chr2.1:55202598-55202617 MS.gene073461:intron 40.0%
! TGATGATAGCGATGTTCTTG+AGG + chr2.1:55202890-55202909 MS.gene073461:intron 40.0%
! TTTTTGCACGCATTCAAGAC+CGG - chr2.1:55203497-55203516 None:intergenic 40.0%
!! AGCAGTTGATGACACACTAT+AGG + chr2.1:55203363-55203382 MS.gene073461:intron 40.0%
!! GCAGTTGATGACACACTATA+GGG + chr2.1:55203364-55203383 MS.gene073461:intron 40.0%
!! GTTGTTGGTTCTGGTAATTC+AGG + chr2.1:55202102-55202121 MS.gene073461:CDS 40.0%
!! TCTATCCTTGTTGTTGGTTC+TGG + chr2.1:55202093-55202112 MS.gene073461:CDS 40.0%
!! TGGTCTCATTTGAGTTGTGA+CGG - chr2.1:55204298-55204317 None:intergenic 40.0%
!!! GAGTGTTTTAACGAGATGAG+AGG - chr2.1:55205631-55205650 None:intergenic 40.0%
AACCGTTACACAAACGAACC+AGG + chr2.1:55202000-55202019 MS.gene073461:CDS 45.0%
ATCAGGGTCCAGTAAATGTG+AGG - chr2.1:55205595-55205614 None:intergenic 45.0%
CTTATGAGAGGATCTCTCTC+TGG - chr2.1:55205405-55205424 None:intergenic 45.0%
GACACACTATAGGGTTCTCA+AGG + chr2.1:55203373-55203392 MS.gene073461:intron 45.0%
GCAACAGGTACACAAGCATA+TGG + chr2.1:55201958-55201977 MS.gene073461:CDS 45.0%
GCCCACATGCATAACACAAA+AGG - chr2.1:55202265-55202284 None:intergenic 45.0%
GTCTTAGCATTTCGCCTAAC+CGG + chr2.1:55203475-55203494 MS.gene073461:intron 45.0%
TAGAGATACTACTGCGATCC+TGG - chr2.1:55202619-55202638 None:intergenic 45.0%
TCACTCCTACAGTCCTACTT+AGG + chr2.1:55204250-55204269 MS.gene073461:intron 45.0%
TCAGGGTCCAGTAAATGTGA+GGG - chr2.1:55205594-55205613 None:intergenic 45.0%
TGAAGAATCACTCGCCTGTT+AGG - chr2.1:55204506-55204525 None:intergenic 45.0%
TGAGTTTGGCAAGATCACAC+TGG - chr2.1:55202690-55202709 None:intergenic 45.0%
TGGTACCAATCAAAAGGACC+TGG - chr2.1:55202021-55202040 None:intergenic 45.0%
TGTGAATTGGACCCGATGAT+GGG + chr2.1:55205567-55205586 MS.gene073461:intron 45.0%
TTAAGACGACGTTCGTGTAG+TGG - chr2.1:55204695-55204714 None:intergenic 45.0%
TTGTTTGCAGCGTTCAGTCA+AGG + chr2.1:55202323-55202342 MS.gene073461:intron 45.0%
! ACGAACCAGGTCCTTTTGAT+TGG + chr2.1:55202013-55202032 MS.gene073461:CDS 45.0%
! GACCTGGTTCGTTTGTGTAA+CGG - chr2.1:55202005-55202024 None:intergenic 45.0%
! GAGGAAACTGATAGAGCATC+AGG - chr2.1:55205612-55205631 None:intergenic 45.0%
!! AGTCAAGGCATGGTTGATGA+AGG + chr2.1:55202338-55202357 MS.gene073461:CDS 45.0%
!! CGTGTCACTTCACTTATGAG+AGG - chr2.1:55205417-55205436 None:intergenic 45.0%
!! GGTCTCATTTGAGTTGTGAC+GGG - chr2.1:55204297-55204316 None:intergenic 45.0%
!! GGTTGATGAAGGTGGTTACA+AGG + chr2.1:55202349-55202368 MS.gene073461:CDS 45.0%
!! GTCTCATTTGAGTTGTGACG+GGG - chr2.1:55204296-55204315 None:intergenic 45.0%
!!! AATTTTATAAAATTATAACA+TGG - chr2.1:55202854-55202873 None:intergenic 5.0%
AAATGTGAGGGGCCCATCAT+CGG - chr2.1:55205582-55205601 None:intergenic 50.0%
AGAGGATCTCTCTCTGGTCT+TGG - chr2.1:55205399-55205418 None:intergenic 50.0%
AGCTCTGTGGATGAAACCCT+AGG + chr2.1:55203930-55203949 MS.gene073461:CDS 50.0%
CAGGGTCCAGTAAATGTGAG+GGG - chr2.1:55205593-55205612 None:intergenic 50.0%
CTCCTACAGTCCTACTTAGG+AGG + chr2.1:55204253-55204272 MS.gene073461:intron 50.0%
CTCTGCACATATCACGGAAG+TGG - chr2.1:55203835-55203854 None:intergenic 50.0%
GACATCGGGATTCAGTCCTA+GGG - chr2.1:55203949-55203968 None:intergenic 50.0%
GTCCTCCTAAGTAGGACTGT+AGG - chr2.1:55204258-55204277 None:intergenic 50.0%
GTGTGAATTGGACCCGATGA+TGG + chr2.1:55205566-55205585 MS.gene073461:intron 50.0%
TCTCGAGAGAGAAGCAAGAC+AGG - chr2.1:55205221-55205240 None:intergenic 50.0%
TGCTACGTGATTCATGCTCG+TGG + chr2.1:55203557-55203576 MS.gene073461:CDS 50.0%
!! CAAGGCATGGTTGATGAAGG+TGG + chr2.1:55202341-55202360 MS.gene073461:CDS 50.0%
!! GGTTTTGTCAGCTCCCAAAG+TGG - chr2.1:55203888-55203907 None:intergenic 50.0%
AATGTGAGGGGCCCATCATC+GGG - chr2.1:55205581-55205600 None:intergenic 55.0%
CTGTAGGAGTGACACCTCAC+TGG - chr2.1:55204242-55204261 None:intergenic 55.0%
GATGGGCCCCTCACATTTAC+TGG + chr2.1:55205584-55205603 MS.gene073461:intron 55.0%
GCACGCATTCAAGACCGGTT+AGG - chr2.1:55203492-55203511 None:intergenic 55.0%
GGACATCGGGATTCAGTCCT+AGG - chr2.1:55203950-55203969 None:intergenic 55.0%
TGCAGCGTTCAGTCAAGGCA+TGG + chr2.1:55202328-55202347 MS.gene073461:intron 55.0%
! GACGACGTTCGTGTAGTGGA+AGG - chr2.1:55204691-55204710 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 55201955 55205948 55201955 ID=MS.gene073461
chr2.1 mRNA 55201955 55205948 55201955 ID=MS.gene073461.t1;Parent=MS.gene073461
chr2.1 exon 55201955 55202123 55201955 ID=MS.gene073461.t1.exon1;Parent=MS.gene073461.t1
chr2.1 CDS 55201955 55202123 55201955 ID=cds.MS.gene073461.t1;Parent=MS.gene073461.t1
chr2.1 exon 55202333 55202449 55202333 ID=MS.gene073461.t1.exon2;Parent=MS.gene073461.t1
chr2.1 CDS 55202333 55202449 55202333 ID=cds.MS.gene073461.t1;Parent=MS.gene073461.t1
chr2.1 exon 55202939 55203007 55202939 ID=MS.gene073461.t1.exon3;Parent=MS.gene073461.t1
chr2.1 CDS 55202939 55203007 55202939 ID=cds.MS.gene073461.t1;Parent=MS.gene073461.t1
chr2.1 exon 55203116 55203135 55203116 ID=MS.gene073461.t1.exon4;Parent=MS.gene073461.t1
chr2.1 CDS 55203116 55203135 55203116 ID=cds.MS.gene073461.t1;Parent=MS.gene073461.t1
chr2.1 exon 55203216 55203268 55203216 ID=MS.gene073461.t1.exon5;Parent=MS.gene073461.t1
chr2.1 CDS 55203216 55203268 55203216 ID=cds.MS.gene073461.t1;Parent=MS.gene073461.t1
chr2.1 exon 55203385 55203415 55203385 ID=MS.gene073461.t1.exon6;Parent=MS.gene073461.t1
chr2.1 CDS 55203385 55203415 55203385 ID=cds.MS.gene073461.t1;Parent=MS.gene073461.t1
chr2.1 exon 55203523 55203601 55203523 ID=MS.gene073461.t1.exon7;Parent=MS.gene073461.t1
chr2.1 CDS 55203523 55203601 55203523 ID=cds.MS.gene073461.t1;Parent=MS.gene073461.t1
chr2.1 exon 55203853 55203992 55203853 ID=MS.gene073461.t1.exon8;Parent=MS.gene073461.t1
chr2.1 CDS 55203853 55203992 55203853 ID=cds.MS.gene073461.t1;Parent=MS.gene073461.t1
chr2.1 exon 55205916 55205948 55205916 ID=MS.gene073461.t1.exon9;Parent=MS.gene073461.t1
chr2.1 CDS 55205916 55205948 55205916 ID=cds.MS.gene073461.t1;Parent=MS.gene073461.t1
Gene Sequence

>MS.gene073461

ATGGCAACAGGTACACAAGCATATGGTGAATCATGGTACTGGGACAACCGTTACACAAACGAACCAGGTCCTTTTGATTGGTACCAAAAGTATATTACTTTGGCTCCTATCATCAATCTCTATGTTCCCAAGAACCAATCTATCCTTGTTGTTGGTTCTGGTAATTCAGGTAAGTCAGTCCAAAAAACAAAACATTTTTTTTTTCATTTCTTAGATCTCTCTTATATGATATTTAATGCTACCCCATTTATTCTTTTTCATTTGGGTTCAATAAAAATCTTTTTTTTTTTTAGAATTTTTTTTATCACCTTTTGTGTTATGCATGTGGGCATGTAAATTTCAAGGAAATGAAAATTTTTGTGTTTGTGTTGTTTGCAGCGTTCAGTCAAGGCATGGTTGATGAAGGTGGTTACAAGGATGTTGTGAACATTGATATTTCTTCAGTGGTCATTGATGCTATGCAGAAGAAATACCGAGATCGTCCTCAATTGAAGTGTATCCTTATGTTGATTAATTCATGTTATATACCATGTTTTTAAATTATGAAGTTGGATCTTAATTTTTCATACTATATTATATTTATAGAGAATTGTGGTCAAATGCGGCTTATACTGTGATCATTAAGACATCAAAGTGTATGTTTGGATTCACAATGATTTTGCCAGGATCGCAGTAGTATCTCTAGTGTGATTTTGGCAAAATCTATACTTTGATGTTTCAACAAGATCACAGCCAGTGTGATCTTGCCAAACTCACCATCAAACTAAACATGTATAAAAATATTTATTGCGGCTCTTTTTAAAACCTTTGTATAAATATATTGTTTTTTCATTTTCACGCTACTACAATTGTCATTAGAAAAAGTATGCATTATTTTCCAATTTTCCATGTTATATCCATGTTATAATTTTATAAAATTTTGCTGAAGTGGTGAATGATGATAGCGATGTTCTTGAGGCTGAAACATCCTTAATAGTTAGACAGATTTGAAAATGGATGTCAGAGACATGAGTGCTTTTGCATCAGAGACTTTTGGTTCTGTTATTGACAAAGGTTTTCTTCCCATTCATTCATAGATTCATTTATTTTTGAATAAATAATATAATTAGATTAACTCTTGAACAACGAATCGTTTTGATATTGTTTAATTTTGTTTTTCAGGAACTCTTGATTCTATCTTGGTGAGTGATGATGTTCATTGAAATACTCGTGTGTTTGACAAGACTAACAGTTTGCTCATTGGATATTTTTGTTGTACTAGTGTGGAAACAATTCAAGACAACATGCTACTAAGATGCTTGAGGAAATTTGGAGGTATAATAAAACCATGAACAAAAAGTCCATAGTTCTTTACCACTTCTGTTTCATTTTGGATTTTTTTTATTCTTGATCTGTTCACCATTATTGAAGCAGTTGATGACACACTATAGGGTTCTCAAGGCTAAAGGAGTCTATATTCTGGTAAGCGAACCTCTTAGTACCGAGTTGTATTCAGATTTATTCTACAATAGATGAAACATGTCTTAGCATTTCGCCTAACCGGTCTTGAATGCGTGCAAAAATTGCAGGTGACGTATGGAGCTCCACTATATCGCTTACGTTTGCTACGTGATTCATGCTCGTGGACAATTAAACTCCATGTAATAGGTTGGTTTAAATCCTCTTATCATTGTCATTTTTTGTGCCAAGTTAACACATGAACTGTTCTTATATTATATCTTTAATAATATATACCATTTTTCTAAAAAAAACATTTCCCTTTGAAGCATCCTATTTATGTGTTCCTTTATAAATTTGCGCCTCTTCGGTCAAATATATAGGCATTGTTTTGCCTAGTTCATTCATATCTGGAGTGTTAATTAAGTCATTGTCAATAACCACTTCCGTGATATGTGCAGAGAAACTTGCATCAGAAGAAAAATCTGATAATCCACTTTGGGAGCTGACAAAACCTATTCCACTTAATAATGATGGAAGCTCTGTGGATGAAACCCTAGGACTGAATCCCGATGTCCATTATATATACATTTGCACTAAGGTAATCAGATGATTTTTCGTACTTACTCTTTTATGTTCTTGGTCAATAGTGTTGGTATAAAGAGCTAAAACATTATTGCCTTTTTTTTGAGAGTTGTCTAATCATAAATTTTACCAAACGAAGGCAACTTTAACAATATTAGCAATAAAAAGTTGTAGAAAAATAAATAAGATTGCCGCAAATTTGAAGTGGAAAATCCCTTCAATGTAGAATAATAAAAATCACTAGATTCTTTTTTGAAGATAGCCAGTGAGGTGTCACTCCTACAGTCCTACTTAGGAGGACTTACTTGACAAATCTCCCCGTCACAACTCAAATGAGACCAAACCTTGATATCATAGCAATTGTGCAACAAGTCTCAAAATTCTCTCCCTAAGGCAATTTCACTGAATCATAAATATGATTCTCATATATGGCTTCCGTCCACTCTTCGTTATGCTTATCTTATGTAGTTTCATCCAAATGTTTTGACTCTCCTTCAAAATGCATCGAGTCAAAAAAATTTAGTTTCCTAACAGGCGAGTGATTCTTCAAAGAAATGTTAGTTTGCTCTCCTATAAAATAGCCAACAAAATTATGCATTTGCGGATTTCCTATAATAGGGAACACATCTCATTTGACTAGCTCAACCATGACTTTCTAATTCATGCAACACAATCTATTATTATATCGTAATAACACCTTATTAGTTGATTCCTTCCACTACACGAACGTCGTCTTAAATGCATATTACTTTAATTAGTGTCATGTAAATGGTAGAATATAATGTACCTCTAGGAACATATGTAGAACCATTCGTGAGTTTCCAATCATTCTATAAGAACAAGCTGTCATATTAACACATAGCTTAGTCCCTAAAAGCAAATTTGGTTGGATATATAAAACATACTTCACAAGCTATCACTAAAGAACTAAGAATGTCCCTTCTAAGCTTAACATGCAATGAAGGACCATTGTTGATAATCTAGTTGAATCTGTTATCAACAATATTAATAAAATGGGGGAAGGAAATCATTAGATGCAGCAATATCTTTGTCTTCAATAATCAATAGTAATGTCTTTTTTTTGCCCTTTATTACTGTGTTTCTTCTCCTTTTGAAAGTTGGCAGTACTTCTAAATGACTAAATATGTGATTTATTTACTACGATGGTGGCATTTCTACATTTTTGTTTTACTTATTTTTAGATTTACTTTTGACTTCGTCTCTGTCAGATGTCTTGAATTCCTGTCTTGCTTCTCTCTCGAGACTCAATAACCAATACCCTTTAGAGTGAAGAAGATTTTGCATGATTCCGATAGAAACTAGAAAACCGAAGTGAAATTGTGCATTAGTGACTCTGATAATGGATGAGCTTGAAGCTCTTTACAATGGTGGAAATATGGCAACAGAGTTCTAAGAACGCCAAGACCAGAGAGAGATCCTCTCATAAGTGAAGTGACACGAACTAACTTTCTTCAACGATTCACTTACTAACCTTCCCTCTCTTTATAAAAGAAATTCTCCTAGACTTCAGGGTATATCCTAACAACCTTTATTCCTTCAGCTTCTTCAATCTTCTTGAAACTGCACTAGTGTGAATTGGACCCGATGATGGGCCCCTCACATTTACTGGACCCTGATGCTCTATCAGTTTCCTCTCATCTCGTTAAAACACTCGTGACTTCTCATCTTGTTGAAAATACTTAGGAGTATATAGTTAGATGTTGATAAAATTTCTCATTCTTTACGTTAAGAAAATCTTCTGTTGTACGTAATTTGAATAATTTAAATTTACCATTCAGTTTTCATTTGTTCCCATTGCCGCTAATTTGAATAATTAATCGTCATGGTTTGAAATTCGTATTTCTGAGTGATGTTGAATGTAGCTGTATGTATCCTTTGCCTATTTAGACTAATCATTCTGTTATAATCTTTCTGACAGGAAATTTCTGCTAACTCAAACGTAAAAGCATGA
Protein sequence

>MS.gene073461.t1

MATGTQAYGESWYWDNRYTNEPGPFDWYQKYITLAPIINLYVPKNQSILVVGSGNSAFSQGMVDEGGYKDVVNIDISSVVIDAMQKKYRDRPQLKYLKMDVRDMSAFASETFGSVIDKGTLDSILCGNNSRQHATKMLEEIWRVLKAKGVYILVTYGAPLYRLRLLRDSCSWTIKLHVIEKLASEEKSDNPLWELTKPIPLNNDGSSVDETLGLNPDVHYIYICTKEISANSNVKA