Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073471.t1 | AES65146.1 | 94.2 | 86 | 5 | 0 | 1 | 86 | 1 | 86 | 3.70E-40 | 173.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073471.t1 | G7IG17 | 94.2 | 86 | 5 | 0 | 1 | 86 | 1 | 86 | 2.6e-40 | 173.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049853 | MS.gene073471 | 0.813117 | 3.01E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073471.t1 | MTR_2g035950 | 94.186 | 86 | 5 | 0 | 1 | 86 | 1 | 86 | 4.73e-56 | 167 |
| MS.gene073471.t1 | MTR_2g035990 | 90.698 | 86 | 8 | 0 | 1 | 86 | 1 | 86 | 2.25e-54 | 163 |
| MS.gene073471.t1 | MTR_2g036020 | 87.209 | 86 | 11 | 0 | 1 | 86 | 1 | 86 | 1.32e-52 | 159 |
| MS.gene073471.t1 | MTR_3g094970 | 48.485 | 99 | 37 | 2 | 1 | 85 | 1 | 99 | 2.27e-22 | 83.2 |
| MS.gene073471.t1 | MTR_8g091930 | 42.157 | 102 | 37 | 3 | 6 | 85 | 7 | 108 | 6.57e-18 | 72.0 |
| MS.gene073471.t1 | MTR_8g091930 | 39.252 | 107 | 43 | 3 | 1 | 85 | 3 | 109 | 3.23e-17 | 70.5 |
| MS.gene073471.t1 | MTR_3g094890 | 41.667 | 84 | 43 | 3 | 6 | 86 | 2 | 82 | 2.20e-14 | 62.4 |
| MS.gene073471.t1 | MTR_8g091930 | 57.407 | 54 | 17 | 1 | 6 | 53 | 7 | 60 | 5.46e-13 | 59.3 |
| MS.gene073471.t1 | MTR_8g091930 | 51.724 | 58 | 22 | 1 | 1 | 52 | 3 | 60 | 3.39e-12 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073471.t1 | AT5G64850 | 40.541 | 111 | 39 | 3 | 2 | 85 | 3 | 113 | 1.08e-16 | 69.3 |
| MS.gene073471.t1 | AT5G09960 | 41.441 | 111 | 39 | 5 | 1 | 85 | 1 | 111 | 5.86e-14 | 62.0 |
| MS.gene073471.t1 | AT5G09960 | 40.187 | 107 | 39 | 4 | 4 | 85 | 3 | 109 | 6.10e-14 | 62.0 |
| MS.gene073471.t1 | AT5G19473 | 38.710 | 93 | 44 | 4 | 6 | 85 | 5 | 97 | 1.05e-12 | 58.5 |
| MS.gene073471.t1 | AT5G19473 | 38.043 | 92 | 44 | 4 | 7 | 85 | 41 | 132 | 1.86e-11 | 56.2 |
Find 19 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTGCCGCGGTTTGGGGAAT+GGG | 0.361355 | 2.1:-55101496 | MS.gene073471:CDS |
| CAGTGCCGCGGTTTGGGGAA+TGG | 0.363664 | 2.1:-55101497 | MS.gene073471:CDS |
| CATTCCCCAAACCGCGGCAC+TGG | 0.366389 | 2.1:+55101498 | None:intergenic |
| TCCATTGAATAATCTCGTAC+TGG | 0.439206 | 2.1:+55101462 | None:intergenic |
| AGGAACTCAAGGCTTCATTC+TGG | 0.446413 | 2.1:-55101386 | MS.gene073471:CDS |
| ATTCCCCAAACCGCGGCACT+GGG | 0.449762 | 2.1:+55101499 | None:intergenic |
| TCCCCAAACCGCGGCACTGG+GGG | 0.495053 | 2.1:+55101501 | None:intergenic |
| CCCAGTACGAGATTATTCAA+TGG | 0.537136 | 2.1:-55101463 | MS.gene073471:CDS |
| ACACAAGAGTCTTGGTAATG+AGG | 0.537488 | 2.1:-55101409 | MS.gene073471:CDS |
| GAAGGAAGAGCTTCATGAAG+TGG | 0.549945 | 2.1:-55101073 | MS.gene073471:CDS |
| CCATTGAATAATCTCGTACT+GGG | 0.551099 | 2.1:+55101463 | None:intergenic |
| AGAAAACTACACAAGAGTCT+TGG | 0.557677 | 2.1:-55101417 | MS.gene073471:CDS |
| GTTTGGGGAATGGGATCAGA+AGG | 0.558166 | 2.1:-55101487 | MS.gene073471:CDS |
| TGGTAATGAGGAGGAACTCA+AGG | 0.571983 | 2.1:-55101397 | MS.gene073471:CDS |
| TTCCCCAAACCGCGGCACTG+GGG | 0.602803 | 2.1:+55101500 | None:intergenic |
| TGTCATGGAACGACATACAA+AGG | 0.642113 | 2.1:-55102394 | None:intergenic |
| CAAGAGTCTTGGTAATGAGG+AGG | 0.661344 | 2.1:-55101406 | MS.gene073471:CDS |
| TTTGGGGAATGGGATCAGAA+GGG | 0.675808 | 2.1:-55101486 | MS.gene073471:CDS |
| TGATCCCATTCCCCAAACCG+CGG | 0.684518 | 2.1:+55101492 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTTTTTTTTTTTTTTT+AGG | - | chr2.1:55101531-55101550 | MS.gene073471:intron | 0.0% |
| !!! | CAAATTATTTTTTGTATAAT+CGG | + | chr2.1:55101131-55101150 | None:intergenic | 10.0% |
| !! | AAAATGAATAAATAATAGAG+AGG | + | chr2.1:55101087-55101106 | None:intergenic | 15.0% |
| !! | AAATCTCTTATAAATATGAT+CGG | - | chr2.1:55101774-55101793 | MS.gene073471:intron | 15.0% |
| !! | AAATGAATAAATAATAGAGA+GGG | + | chr2.1:55101086-55101105 | None:intergenic | 15.0% |
| !! | ATACAAAAAATAATTTGGTT+TGG | - | chr2.1:55101134-55101153 | MS.gene073471:intron | 15.0% |
| !! | ATCAAAGTTAAATGATTTAT+AGG | + | chr2.1:55101899-55101918 | None:intergenic | 15.0% |
| !! | ATGTCTCTTATAAATAAAAT+CGG | + | chr2.1:55101686-55101705 | None:intergenic | 15.0% |
| !! | CGATTATACAAAAAATAATT+TGG | - | chr2.1:55101129-55101148 | MS.gene073471:intron | 15.0% |
| !!! | ATCATATTTATAAGAGATTT+TGG | + | chr2.1:55101774-55101793 | None:intergenic | 15.0% |
| !!! | TACAAAAAATAATTTGGTTT+GGG | - | chr2.1:55101135-55101154 | MS.gene073471:intron | 15.0% |
| !!! | TCATATTTATAAGAGATTTT+GGG | + | chr2.1:55101773-55101792 | None:intergenic | 15.0% |
| !! | TCAACAAAAGTTAATGTATA+TGG | + | chr2.1:55101750-55101769 | None:intergenic | 20.0% |
| ! | TGATGAAACTAATCAGTAAT+TGG | + | chr2.1:55101655-55101674 | None:intergenic | 25.0% |
| !! | AAATCATTTAACTTTGATGC+AGG | - | chr2.1:55101901-55101920 | MS.gene073471:intron | 25.0% |
| !!! | AGTAGAAAAGCATTTTATAG+CGG | - | chr2.1:55101194-55101213 | MS.gene073471:intron | 25.0% |
| !!! | TTCAGTTACCTTTGTTATTA+GGG | - | chr2.1:55102126-55102145 | MS.gene073471:intron | 25.0% |
| !!! | TTTCAGTTACCTTTGTTATT+AGG | - | chr2.1:55102125-55102144 | MS.gene073471:intron | 25.0% |
| !!! | TTTTTTTCTCTTTACAGAGA+AGG | - | chr2.1:55102348-55102367 | MS.gene073471:intron | 25.0% |
| AACTCATTCTCTCACAAAAT+TGG | + | chr2.1:55101401-55101420 | None:intergenic | 30.0% | |
| AAGGTAACTGAAAATCTTAC+AGG | + | chr2.1:55102118-55102137 | None:intergenic | 30.0% | |
| ATTAATAGAACACCCTTCAA+TGG | + | chr2.1:55101438-55101457 | None:intergenic | 30.0% | |
| CTTATTGAGCTGTAAATTAG+TGG | + | chr2.1:55102173-55102192 | None:intergenic | 30.0% | |
| TATGAATCTTTGCATCCAAA+TGG | - | chr2.1:55102257-55102276 | MS.gene073471:intron | 30.0% | |
| TGAAACCAGTTAAATCCATT+TGG | + | chr2.1:55102275-55102294 | None:intergenic | 30.0% | |
| TGATTGATTGAACCATTGAA+GGG | - | chr2.1:55101423-55101442 | MS.gene073471:CDS | 30.0% | |
| TTGATTGATTGAACCATTGA+AGG | - | chr2.1:55101422-55101441 | MS.gene073471:CDS | 30.0% | |
| AATGTCGAACCTGCAAAAAA+AGG | - | chr2.1:55101361-55101380 | MS.gene073471:CDS | 35.0% | |
| AGAAAACTACACAAGAGTCT+TGG | - | chr2.1:55102022-55102041 | MS.gene073471:intron | 35.0% | |
| CCATTGAATAATCTCGTACT+GGG | + | chr2.1:55101979-55101998 | None:intergenic | 35.0% | |
| GTAACTGAAAATCTTACAGG+TGG | + | chr2.1:55102115-55102134 | None:intergenic | 35.0% | |
| TAAAAGAGAAGAACCTGCAT+GGG | + | chr2.1:55101583-55101602 | None:intergenic | 35.0% | |
| TACAGCTCAATAAGATGAGA+TGG | - | chr2.1:55102179-55102198 | MS.gene073471:intron | 35.0% | |
| TATAGCGGTCAGTAAATTCA+CGG | - | chr2.1:55101209-55101228 | MS.gene073471:intron | 35.0% | |
| TCCATTGAATAATCTCGTAC+TGG | + | chr2.1:55101980-55101999 | None:intergenic | 35.0% | |
| ! | TGCATCCAAATGGATTTAAC+TGG | - | chr2.1:55102267-55102286 | MS.gene073471:intron | 35.0% |
| ! | TGTGTAGTTTTCTTGCTTCT+TGG | + | chr2.1:55102015-55102034 | None:intergenic | 35.0% |
| !! | AGTTACCTTTGTTATTAGGG+CGG | - | chr2.1:55102129-55102148 | MS.gene073471:intron | 35.0% |
| ACAAGAAGAGCCATTACAAG+CGG | + | chr2.1:55101512-55101531 | None:intergenic | 40.0% | |
| AGTGGTCGTCAAATCTTGAT+CGG | + | chr2.1:55101341-55101360 | None:intergenic | 40.0% | |
| ATCGGCTGATAGTTTCAGAA+AGG | + | chr2.1:55101113-55101132 | None:intergenic | 40.0% | |
| CCCAGTACGAGATTATTCAA+TGG | - | chr2.1:55101976-55101995 | MS.gene073471:intron | 40.0% | |
| CTAAAAGAGAAGAACCTGCA+TGG | + | chr2.1:55101584-55101603 | None:intergenic | 40.0% | |
| ! | CATGCAGGTTCTTCTCTTTT+AGG | - | chr2.1:55101582-55101601 | MS.gene073471:intron | 40.0% |
| ! | TTTTGCAGGTTCGACATTAG+TGG | + | chr2.1:55101359-55101378 | None:intergenic | 40.0% |
| !! | ACACAAGAGTCTTGGTAATG+AGG | - | chr2.1:55102030-55102049 | MS.gene073471:intron | 40.0% |
| !! | AGGTTTTTGAAAACCCATGC+AGG | - | chr2.1:55101567-55101586 | MS.gene073471:intron | 40.0% |
| AACTATGCCTCCGCTTGTAA+TGG | - | chr2.1:55101499-55101518 | MS.gene073471:CDS | 45.0% | |
| AGGAACTCAAGGCTTCATTC+TGG | - | chr2.1:55102053-55102072 | MS.gene073471:intron | 45.0% | |
| GAAGGAAGAGCTTCATGAAG+TGG | - | chr2.1:55102366-55102385 | MS.gene073471:intron | 45.0% | |
| GTCGTCAAATCTTGATCGGA+TGG | + | chr2.1:55101337-55101356 | None:intergenic | 45.0% | |
| TCTCTCCGCCCTAATAACAA+AGG | + | chr2.1:55102137-55102156 | None:intergenic | 45.0% | |
| TTTGGGGAATGGGATCAGAA+GGG | - | chr2.1:55101953-55101972 | MS.gene073471:intron | 45.0% | |
| ! | TGGTAATGAGGAGGAACTCA+AGG | - | chr2.1:55102042-55102061 | MS.gene073471:intron | 45.0% |
| !! | CAAGAGTCTTGGTAATGAGG+AGG | - | chr2.1:55102033-55102052 | MS.gene073471:intron | 45.0% |
| !!! | ATGCAGTCGCCTTTTTTTGC+AGG | + | chr2.1:55101373-55101392 | None:intergenic | 45.0% |
| AGAAGAGCCATTACAAGCGG+AGG | + | chr2.1:55101509-55101528 | None:intergenic | 50.0% | |
| GTTTGGGGAATGGGATCAGA+AGG | - | chr2.1:55101952-55101971 | MS.gene073471:intron | 50.0% | |
| TGATCCCATTCCCCAAACCG+CGG | + | chr2.1:55101950-55101969 | None:intergenic | 55.0% | |
| ATTCCCCAAACCGCGGCACT+GGG | + | chr2.1:55101943-55101962 | None:intergenic | 60.0% | |
| ! | AGTGCCGCGGTTTGGGGAAT+GGG | - | chr2.1:55101943-55101962 | MS.gene073471:intron | 60.0% |
| CATTCCCCAAACCGCGGCAC+TGG | + | chr2.1:55101944-55101963 | None:intergenic | 65.0% | |
| TTCCCCAAACCGCGGCACTG+GGG | + | chr2.1:55101942-55101961 | None:intergenic | 65.0% | |
| ! | CAGTGCCGCGGTTTGGGGAA+TGG | - | chr2.1:55101942-55101961 | MS.gene073471:intron | 65.0% |
| GTCCCCCAGTGCCGCGGTTT+GGG | - | chr2.1:55101936-55101955 | MS.gene073471:intron | 70.0% | |
| TCCCCCAGTGCCGCGGTTTG+GGG | - | chr2.1:55101937-55101956 | MS.gene073471:intron | 70.0% | |
| TGTCCCCCAGTGCCGCGGTT+TGG | - | chr2.1:55101935-55101954 | MS.gene073471:intron | 70.0% | |
| ! | AACCGCGGCACTGGGGGACA+AGG | + | chr2.1:55101935-55101954 | None:intergenic | 70.0% |
| ! | ACCGCGGCACTGGGGGACAA+GGG | + | chr2.1:55101934-55101953 | None:intergenic | 70.0% |
| ! | TCCCCAAACCGCGGCACTGG+GGG | + | chr2.1:55101941-55101960 | None:intergenic | 70.0% |
| GCCCTTGTCCCCCAGTGCCG+CGG | - | chr2.1:55101930-55101949 | MS.gene073471:intron | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 55101049 | 55102412 | 55101049 | ID=MS.gene073471 |
| chr2.1 | mRNA | 55101049 | 55102412 | 55101049 | ID=MS.gene073471.t1;Parent=MS.gene073471 |
| chr2.1 | exon | 55102395 | 55102412 | 55102395 | ID=MS.gene073471.t1.exon1;Parent=MS.gene073471.t1 |
| chr2.1 | CDS | 55102395 | 55102412 | 55102395 | ID=cds.MS.gene073471.t1;Parent=MS.gene073471.t1 |
| chr2.1 | exon | 55101344 | 55101538 | 55101344 | ID=MS.gene073471.t1.exon2;Parent=MS.gene073471.t1 |
| chr2.1 | CDS | 55101344 | 55101538 | 55101344 | ID=cds.MS.gene073471.t1;Parent=MS.gene073471.t1 |
| chr2.1 | exon | 55101049 | 55101096 | 55101049 | ID=MS.gene073471.t1.exon3;Parent=MS.gene073471.t1 |
| chr2.1 | CDS | 55101049 | 55101096 | 55101049 | ID=cds.MS.gene073471.t1;Parent=MS.gene073471.t1 |
| Gene Sequence |
| Protein sequence |
