AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07391

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07391.t1 MTR_1g093120 91.667 168 14 0 1 168 1 168 6.74e-109 318
MS.gene07391.t1 MTR_1g093120 97.590 83 2 0 165 247 288 370 4.81e-49 165
MS.gene07391.t1 MTR_1g093110 79.641 167 32 1 1 165 2 168 1.21e-92 277
MS.gene07391.t1 MTR_1g093110 76.190 84 19 1 165 247 291 374 3.78e-36 131
MS.gene07391.t1 MTR_1g093140 77.059 170 33 3 1 164 1 170 1.58e-89 270
MS.gene07391.t1 MTR_1g093140 85.882 85 10 1 165 247 294 378 2.73e-43 150
MS.gene07391.t1 MTR_4g024660 47.083 240 107 7 1 232 1 228 2.82e-63 202
MS.gene07391.t1 MTR_4g024660 62.791 86 29 1 165 247 288 373 1.09e-29 114
MS.gene07391.t1 MTR_8g062600 46.250 240 109 7 1 232 1 228 3.94e-63 202
MS.gene07391.t1 MTR_8g062600 66.279 86 26 1 165 247 288 373 6.53e-31 117
MS.gene07391.t1 MTR_7g078010 62.179 156 53 3 9 163 13 163 9.71e-62 196
MS.gene07391.t1 MTR_7g056347 49.356 233 92 8 9 232 13 228 9.94e-62 198
MS.gene07391.t1 MTR_7g056347 60.465 86 31 1 165 247 288 373 1.08e-28 112
MS.gene07391.t1 MTR_8g006930 60.645 155 57 2 9 163 13 163 1.82e-61 197
MS.gene07391.t1 MTR_8g006930 63.953 86 28 1 165 247 288 373 1.40e-31 119
MS.gene07391.t1 MTR_4g119410 60.645 155 57 2 9 163 13 163 2.79e-61 197
MS.gene07391.t1 MTR_4g119410 69.767 86 23 1 165 247 288 373 1.81e-34 127
MS.gene07391.t1 MTR_7g078010 62.179 156 53 3 9 163 13 163 1.51e-60 195
MS.gene07391.t1 MTR_7g078010 56.977 86 34 1 165 247 288 373 2.20e-25 102
MS.gene07391.t1 MTR_8g063070 45.306 245 104 7 1 232 1 228 3.60e-59 191
MS.gene07391.t1 MTR_8g063070 59.302 86 32 1 165 247 288 373 2.07e-25 103
MS.gene07391.t1 MTR_7g056393 58.025 162 62 3 2 163 8 163 2.95e-58 189
MS.gene07391.t1 MTR_7g056393 67.442 86 25 1 165 247 288 373 7.77e-34 125
MS.gene07391.t1 MTR_2g069020 53.846 156 70 1 9 164 11 164 3.98e-56 184
MS.gene07391.t1 MTR_2g069020 63.095 84 29 2 165 247 288 370 1.07e-23 98.2
MS.gene07391.t1 MTR_2g069010 53.846 156 70 1 9 164 13 166 9.61e-56 183
MS.gene07391.t1 MTR_2g069010 60.920 87 29 2 165 247 290 375 1.99e-25 103
MS.gene07391.t1 MTR_2g069020 53.846 156 70 1 9 164 11 164 1.15e-55 183
MS.gene07391.t1 MTR_2g069020 63.095 84 29 2 165 247 288 370 1.46e-23 98.2
MS.gene07391.t1 MTR_4g099380 60.000 150 51 4 15 163 124 265 2.58e-55 179
MS.gene07391.t1 MTR_2g068960 50.968 155 74 1 10 164 8 160 8.42e-53 175
MS.gene07391.t1 MTR_2g068960 55.172 87 34 2 165 247 284 369 7.45e-22 93.2
MS.gene07391.t1 MTR_2g068950 51.974 152 71 1 13 164 11 160 9.15e-53 175
MS.gene07391.t1 MTR_2g068950 56.322 87 33 2 165 247 284 369 1.73e-22 95.1
MS.gene07391.t1 MTR_8g469400 44.915 236 105 6 1 232 1 215 6.39e-52 172
MS.gene07391.t1 MTR_8g469400 66.279 86 26 1 165 247 275 360 6.25e-32 120
MS.gene07391.t1 MTR_7g056370 54.676 139 59 2 25 163 8 142 2.84e-47 156
MS.gene07391.t1 MTR_2g069070 47.904 167 63 3 2 168 8 150 6.83e-46 152
MS.gene07391.t1 MTR_8g009080 47.879 165 83 2 4 168 34 195 2.42e-44 152
MS.gene07391.t1 MTR_1g070185 50.331 151 70 3 13 163 18 163 2.60e-44 153
MS.gene07391.t1 MTR_1g070185 47.619 84 39 2 165 247 288 367 6.45e-15 73.6
MS.gene07391.t1 MTR_8g009080 47.879 165 83 2 4 168 34 195 3.33e-44 152
MS.gene07391.t1 MTR_8g009080 47.879 165 83 2 4 168 34 195 1.24e-43 152
MS.gene07391.t1 MTR_8g009080 54.217 83 38 0 165 247 315 397 1.09e-23 98.6
MS.gene07391.t1 MTR_8g041620 45.806 155 79 3 13 165 17 168 1.79e-43 151
MS.gene07391.t1 MTR_8g041620 52.381 84 39 1 165 247 291 374 6.97e-22 93.6
MS.gene07391.t1 MTR_8g009090 45.238 168 84 4 3 168 1 162 1.81e-43 150
MS.gene07391.t1 MTR_8g009090 51.807 83 40 0 165 247 282 364 4.38e-23 96.7
MS.gene07391.t1 MTR_2g069060 47.305 167 64 3 2 168 8 150 2.08e-43 150
MS.gene07391.t1 MTR_2g069060 60.241 83 30 2 165 247 270 349 1.48e-23 97.4
MS.gene07391.t1 MTR_8g041600 45.098 153 79 3 12 162 16 165 3.23e-43 148
MS.gene07391.t1 MTR_8g008930 45.509 167 82 5 3 166 1 161 4.40e-43 149
MS.gene07391.t1 MTR_8g008930 50.602 83 41 0 165 247 283 365 3.48e-22 94.0
MS.gene07391.t1 MTR_8g028435 46.053 152 75 3 18 168 17 162 9.24e-43 149
MS.gene07391.t1 MTR_8g028435 54.217 83 38 0 165 247 282 364 2.07e-24 100
MS.gene07391.t1 MTR_8g041600 44.231 156 82 3 12 165 16 168 2.70e-42 148
MS.gene07391.t1 MTR_8g041600 55.952 84 36 1 165 247 291 374 1.90e-24 100
MS.gene07391.t1 MTR_2g069090 46.358 151 70 3 14 160 18 161 7.74e-42 146
MS.gene07391.t1 MTR_2g069090 53.012 83 39 0 165 247 290 372 5.33e-23 96.3
MS.gene07391.t1 MTR_8g009110 37.555 229 109 9 17 232 16 223 7.84e-42 146
MS.gene07391.t1 MTR_8g009110 53.012 83 39 0 165 247 283 365 3.24e-24 99.8
MS.gene07391.t1 MTR_8g009170 39.013 223 112 8 18 232 17 223 2.29e-41 143
MS.gene07391.t1 MTR_8g009160 37.333 225 114 8 17 232 16 222 5.84e-41 144
MS.gene07391.t1 MTR_8g009160 51.807 83 40 0 165 247 282 364 9.30e-25 101
MS.gene07391.t1 MTR_8g009170 39.013 223 112 8 18 232 17 223 6.91e-41 144
MS.gene07391.t1 MTR_8g009170 52.381 84 39 1 165 247 283 366 3.74e-24 99.4
MS.gene07391.t1 MTR_2g069210 40.476 168 97 2 1 168 1 165 1.12e-40 143
MS.gene07391.t1 MTR_2g069210 56.627 83 36 0 165 247 285 367 2.74e-23 97.1
MS.gene07391.t1 MTR_2g069190 45.578 147 77 2 14 160 18 161 7.46e-40 141
MS.gene07391.t1 MTR_2g069190 53.012 83 39 0 165 247 289 371 3.03e-22 94.4
MS.gene07391.t1 MTR_8g009120 43.226 155 81 3 18 171 17 165 4.75e-39 139
MS.gene07391.t1 MTR_8g009120 54.545 77 35 0 171 247 277 353 6.53e-21 90.5
MS.gene07391.t1 MTR_8g009150 44.231 156 78 4 18 171 17 165 5.71e-39 138
MS.gene07391.t1 MTR_8g009150 54.217 83 38 0 165 247 263 345 1.93e-23 97.4
MS.gene07391.t1 MTR_1g043760 46.939 147 73 3 14 160 5 146 1.23e-38 137
MS.gene07391.t1 MTR_1g043760 48.684 76 39 0 165 240 274 349 3.88e-15 74.3
MS.gene07391.t1 MTR_2g069060 48.819 127 65 0 42 168 2 128 2.47e-38 134
MS.gene07391.t1 MTR_0032s0040 44.970 169 80 5 5 165 4 167 3.27e-38 137
MS.gene07391.t1 MTR_0032s0040 51.190 84 40 1 165 247 290 373 1.43e-19 87.0
MS.gene07391.t1 MTR_7g029655 39.645 169 94 3 5 170 4 167 8.74e-38 136
MS.gene07391.t1 MTR_7g029655 48.837 86 41 2 165 247 285 370 4.01e-17 80.1
MS.gene07391.t1 MTR_1g043770 34.649 228 121 8 14 232 5 213 9.79e-38 134
MS.gene07391.t1 MTR_2g069300 41.935 155 87 2 14 168 4 155 1.25e-37 135
MS.gene07391.t1 MTR_2g069300 54.217 83 38 0 165 247 276 358 1.55e-21 92.0
MS.gene07391.t1 MTR_4g117880 45.161 155 75 5 17 168 16 163 1.73e-37 135
MS.gene07391.t1 MTR_4g117880 58.065 62 26 0 165 226 283 344 8.41e-18 81.6
MS.gene07391.t1 MTR_1g043770 34.956 226 123 7 14 232 5 213 2.44e-37 134
MS.gene07391.t1 MTR_1g043770 48.193 83 43 0 165 247 273 355 6.53e-19 84.7
MS.gene07391.t1 MTR_7g105070 42.308 156 86 3 10 165 5 156 6.41e-35 128
MS.gene07391.t1 MTR_1g044105 41.509 159 86 5 14 168 5 160 1.32e-34 122
MS.gene07391.t1 MTR_4g099390 63.095 84 29 1 166 247 1 84 6.67e-33 115
MS.gene07391.t1 MTR_1g043810 42.282 149 78 5 14 160 5 147 4.35e-32 120
MS.gene07391.t1 MTR_1g043775 34.934 229 121 10 14 232 5 215 7.05e-32 119
MS.gene07391.t1 MTR_1g043810 42.282 149 78 5 14 160 5 147 8.21e-32 120
MS.gene07391.t1 MTR_1g043810 45.745 94 49 1 154 247 266 357 1.23e-17 81.3
MS.gene07391.t1 MTR_1g043775 34.934 229 121 10 14 232 5 215 1.35e-31 119
MS.gene07391.t1 MTR_1g043775 43.617 94 51 1 154 247 266 357 5.46e-16 76.6
MS.gene07391.t1 MTR_1g032250 40.252 159 90 2 3 161 11 164 2.59e-30 116
MS.gene07391.t1 MTR_1g032220 42.282 149 81 2 13 161 21 164 1.57e-29 114
MS.gene07391.t1 MTR_1g032140 40.523 153 86 2 9 161 10 157 1.55e-28 111
MS.gene07391.t1 MTR_7g056353 58.824 85 32 1 165 246 262 346 4.29e-25 103
MS.gene07391.t1 MTR_7g056353 36.129 155 67 4 12 166 18 140 3.22e-23 98.2
MS.gene07391.t1 MTR_8g009180 52.564 78 37 0 165 242 212 289 3.45e-22 92.8
MS.gene07391.t1 MTR_8g008960 44.681 94 51 1 154 247 10 102 1.29e-21 86.7
MS.gene07391.t1 MTR_2g069130 46.667 105 49 3 71 172 12 112 2.44e-19 81.6
MS.gene07391.t1 MTR_1g032230 33.562 146 73 2 5 150 7 128 3.97e-15 73.2
MS.gene07391.t1 MTR_5g032870 39.326 89 54 0 57 145 28 116 1.00e-13 70.1
MS.gene07391.t1 MTR_1g043840 39.759 83 47 2 78 158 257 338 2.03e-13 68.9
MS.gene07391.t1 MTR_8g024120 31.132 106 71 1 56 161 31 134 3.39e-13 68.6
MS.gene07391.t1 MTR_3g092890 29.371 143 90 3 32 172 5 138 5.33e-13 67.8
MS.gene07391.t1 MTR_1g062110 30.370 135 87 4 28 159 26 156 8.26e-13 67.4
MS.gene07391.t1 MTR_1g062110 30.370 135 87 4 28 159 26 156 1.07e-12 67.0
MS.gene07391.t1 MTR_7g081710 34.862 109 67 2 56 164 41 145 1.64e-12 66.6
MS.gene07391.t1 MTR_5g048570 35.789 95 54 2 39 133 21 108 1.83e-12 66.2
MS.gene07391.t1 MTR_7g102820 30.178 169 106 6 14 176 10 172 1.90e-12 66.6
MS.gene07391.t1 MTR_3g091350 29.299 157 101 6 8 159 2 153 2.00e-12 66.2
MS.gene07391.t1 MTR_5g048740 35.185 108 63 3 29 136 11 111 2.16e-12 66.2
MS.gene07391.t1 MTR_5g032880 39.535 86 44 1 57 142 28 105 2.69e-12 66.6
MS.gene07391.t1 MTR_5g032880 40.000 85 49 2 59 142 368 451 5.98e-11 62.4
MS.gene07391.t1 MTR_7g068650 29.032 155 91 6 20 165 6 150 4.16e-12 65.5
MS.gene07391.t1 MTR_1g048940 28.814 118 78 2 42 158 24 136 1.38e-11 63.9
MS.gene07391.t1 MTR_5g048550 31.788 151 83 7 25 167 2 140 1.46e-11 63.9
MS.gene07391.t1 MTR_3g070860 29.825 114 76 3 20 132 6 116 1.64e-11 63.5
MS.gene07391.t1 MTR_4g015790 27.673 159 97 5 20 165 5 158 4.00e-11 62.4
MS.gene07391.t1 MTR_5g082750 30.216 139 92 4 22 159 7 141 5.50e-11 62.0
MS.gene07391.t1 MTR_5g055690 39.806 103 53 5 29 130 13 107 5.56e-11 61.6
MS.gene07391.t1 MTR_5g055820 32.979 94 58 2 40 133 22 110 8.09e-11 61.6
MS.gene07391.t1 MTR_5g055690 39.806 103 53 5 29 130 13 107 9.14e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07391.t1 AT2G30840 49.682 157 75 2 11 165 3 157 2.71e-47 159
MS.gene07391.t1 AT1G06620 47.531 162 82 2 3 164 1 159 9.31e-47 157
MS.gene07391.t1 AT2G30840 49.682 157 75 2 11 165 3 157 1.21e-46 159
MS.gene07391.t1 AT2G30840 57.143 84 34 2 165 247 280 362 1.02e-19 87.4
MS.gene07391.t1 AT2G30840 49.682 157 75 2 11 165 3 157 1.28e-46 159
MS.gene07391.t1 AT2G30840 56.410 78 32 2 171 247 280 356 8.35e-17 79.0
MS.gene07391.t1 AT2G25450 44.025 159 85 2 10 168 2 156 1.91e-46 158
MS.gene07391.t1 AT2G25450 50.588 85 39 3 165 247 276 359 2.20e-15 75.1
MS.gene07391.t1 AT1G06620 47.531 162 82 2 3 164 1 159 8.53e-46 157
MS.gene07391.t1 AT1G06620 56.410 78 34 0 165 242 283 360 6.58e-22 93.2
MS.gene07391.t1 AT2G30830 43.750 160 84 3 10 168 2 156 6.04e-42 146
MS.gene07391.t1 AT2G30830 52.381 84 38 2 165 247 276 358 4.36e-19 85.5
MS.gene07391.t1 AT1G06645 44.785 163 82 5 3 161 14 172 9.05e-40 141
MS.gene07391.t1 AT1G06645 45.238 84 45 1 165 247 296 379 1.77e-17 81.3
MS.gene07391.t1 AT1G03410 44.586 157 83 3 14 168 5 159 2.39e-39 140
MS.gene07391.t1 AT1G03410 39.286 84 49 2 165 247 279 361 7.49e-11 61.6
MS.gene07391.t1 AT1G03410 44.586 157 83 3 14 168 42 196 3.73e-39 140
MS.gene07391.t1 AT1G03410 39.286 84 49 2 165 247 316 398 7.36e-11 62.0
MS.gene07391.t1 AT1G06650 43.137 153 82 3 13 162 10 160 1.60e-38 136
MS.gene07391.t1 AT1G06650 43.137 153 82 3 13 162 10 160 4.46e-38 137
MS.gene07391.t1 AT1G06650 48.810 84 42 1 165 247 286 369 1.20e-18 84.3
MS.gene07391.t1 AT1G03400 44.371 151 74 2 14 164 5 145 5.58e-38 136
MS.gene07391.t1 AT1G03400 44.048 84 45 2 165 247 269 351 1.09e-11 64.3
MS.gene07391.t1 AT1G03400 44.371 151 74 2 14 164 5 145 1.03e-37 136
MS.gene07391.t1 AT1G03400 41.053 95 51 3 157 247 286 379 8.57e-12 64.7
MS.gene07391.t1 AT5G59540 41.358 162 91 3 9 168 5 164 2.12e-37 133
MS.gene07391.t1 AT1G06640 40.237 169 95 4 3 168 1 166 7.69e-37 131
MS.gene07391.t1 AT5G59540 41.358 162 91 3 9 168 5 164 1.04e-36 133
MS.gene07391.t1 AT5G59540 57.831 83 35 0 165 247 284 366 1.48e-24 100
MS.gene07391.t1 AT3G61400 41.250 160 86 3 14 168 10 166 1.64e-36 130
MS.gene07391.t1 AT1G06640 40.237 169 95 4 3 168 1 166 2.50e-36 131
MS.gene07391.t1 AT1G06640 40.237 169 95 4 3 168 1 166 3.93e-36 131
MS.gene07391.t1 AT1G06640 48.810 84 42 1 165 247 286 369 1.45e-17 81.3
MS.gene07391.t1 AT3G61400 41.250 160 86 3 14 168 10 166 7.35e-36 130
MS.gene07391.t1 AT3G61400 45.570 79 42 1 165 242 288 366 1.04e-14 73.2
MS.gene07391.t1 AT1G04350 38.889 162 93 3 8 168 1 157 1.55e-35 130
MS.gene07391.t1 AT1G04350 53.571 84 38 1 165 247 277 360 7.87e-19 84.7
MS.gene07391.t1 AT5G59530 41.104 163 89 5 9 168 4 162 2.59e-34 126
MS.gene07391.t1 AT5G59530 60.241 83 33 0 165 247 282 364 1.07e-25 103
MS.gene07391.t1 AT5G43440 40.127 157 89 3 15 169 10 163 4.38e-33 122
MS.gene07391.t1 AT5G43440 40.127 157 89 3 15 169 10 163 2.03e-32 121
MS.gene07391.t1 AT5G43440 49.398 83 42 0 165 247 283 365 2.59e-17 80.5
MS.gene07391.t1 AT5G43450 40.506 158 89 3 9 165 4 157 4.75e-32 119
MS.gene07391.t1 AT5G43450 40.506 158 89 3 9 165 4 157 1.85e-31 119
MS.gene07391.t1 AT5G43450 46.154 78 42 0 165 242 280 357 5.50e-14 70.9
MS.gene07391.t1 AT1G04380 39.683 126 70 2 40 165 21 140 1.31e-26 104
MS.gene07391.t1 AT1G04380 39.683 126 70 2 40 165 21 140 4.30e-26 104
MS.gene07391.t1 AT1G04380 59.036 83 34 0 165 247 263 345 3.08e-24 99.4
MS.gene07391.t1 AT3G13610 30.409 171 97 7 5 167 2 158 3.03e-15 74.7
MS.gene07391.t1 AT2G36690 32.468 154 94 4 17 164 7 156 5.14e-15 73.9
MS.gene07391.t1 AT2G36690 32.468 154 94 4 17 164 7 156 5.14e-15 73.9
MS.gene07391.t1 AT1G55290 30.345 145 85 5 28 167 25 158 1.63e-13 69.7
MS.gene07391.t1 AT1G17020 35.514 107 66 2 29 135 17 120 1.67e-13 69.7
MS.gene07391.t1 AT4G10500 34.694 98 62 2 62 159 39 134 4.13e-13 68.2
MS.gene07391.t1 AT5G24530 30.303 132 81 3 32 161 5 127 7.47e-13 67.4
MS.gene07391.t1 AT4G10490 31.915 94 62 2 66 159 41 132 2.06e-12 66.2
MS.gene07391.t1 AT2G44800 33.019 106 70 1 59 164 44 148 2.65e-12 65.9
MS.gene07391.t1 AT2G38240 26.897 145 97 4 21 165 4 139 2.74e-12 65.9
MS.gene07391.t1 AT3G12900 33.333 144 86 8 28 169 21 156 1.52e-11 63.9
MS.gene07391.t1 AT3G60290 34.906 106 68 1 59 164 44 148 1.54e-11 63.9

Find 66 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCGATAACAATTCCTTTAT+TGG 0.087638 1.2:-64379474 None:intergenic
ACTCTTCCTTGCACCCTTTC+AGG 0.130980 1.2:+64379574 MS.gene07391:CDS
ATCGGAGTTCAAGTTCAAAT+TGG 0.263150 1.2:-64378378 None:intergenic
AAGAAAATCCGCCAATCTAT+AGG 0.288921 1.2:+64379496 MS.gene07391:CDS
TGGTATTCCGGTTAGTGTTT+TGG 0.298579 1.2:+64378544 MS.gene07391:CDS
CTTTCTCATCATGGAGTTAG+AGG 0.301119 1.2:+64378223 None:intergenic
TAGCAGCAGGGTCCCTAAAA+AGG 0.302035 1.2:-64378676 None:intergenic
TTAGGGACCCTGCTGCTAAT+TGG 0.334646 1.2:+64378681 MS.gene07391:CDS
AAAGTTCGATATTTCTCTAA+TGG 0.338662 1.2:+64378650 MS.gene07391:CDS
TCCAATAAAGGAATTGTTAT+CGG 0.349838 1.2:+64379473 MS.gene07391:CDS
AGTGATTAATCATGGTATTC+CGG 0.365484 1.2:+64378532 MS.gene07391:CDS
CAAGGTCCTTGGTCCAATAA+AGG 0.378696 1.2:+64379461 MS.gene07391:CDS
CTTGCATCCCTTGTATAGAA+TGG 0.390636 1.2:-64378623 None:intergenic
ATATCTTGAAGGTCTATGAT+AGG 0.390808 1.2:-64378434 None:intergenic
AGAAAATCCGCCAATCTATA+GGG 0.399681 1.2:+64379497 MS.gene07391:CDS
ATCTCATCCAAAACACTAAC+CGG 0.405079 1.2:-64378551 None:intergenic
GCCTTTGAAGAATCAAAATT+CGG 0.411407 1.2:+64378293 MS.gene07391:CDS
TCAAGTTCAAATTGGGAGAA+TGG 0.415040 1.2:-64378370 None:intergenic
TACTACTGTGAAAGAAGTCT+TGG 0.435724 1.2:+64379521 MS.gene07391:CDS
GATTAGTGTTTATATCTTGA+AGG 0.437519 1.2:-64378445 None:intergenic
ACAGTAGTATCCCTATAGAT+TGG 0.448112 1.2:-64379507 None:intergenic
TCGGAGTTCAAGTTCAAATT+GGG 0.456908 1.2:-64378377 None:intergenic
TCTTAATTCACAACCTGAAA+GGG 0.469355 1.2:-64379587 None:intergenic
GTGAAAGGCCTCTTAGAATC+TGG 0.477165 1.2:+64378317 MS.gene07391:CDS
GAATCAAAATTCGGCGTGAA+AGG 0.479516 1.2:+64378302 MS.gene07391:CDS
AAGCTAGCTATGGAAACTCT+AGG 0.481857 1.2:-64379411 None:intergenic
CTTTATTGGACCAAGGACCT+TGG 0.485130 1.2:-64379460 None:intergenic
CAAGGAAACCATTCTATACA+AGG 0.487279 1.2:+64378615 MS.gene07391:CDS
TTCTTAATTCACAACCTGAA+AGG 0.491843 1.2:-64379588 None:intergenic
GAAGATTCGAAGTGCATGTA+AGG 0.497098 1.2:+64378493 MS.gene07391:CDS
TATGATAGGGACATTGAACT+TGG 0.498593 1.2:-64378420 None:intergenic
GAGTCCCTCCAATTAGCAGC+AGG 0.508138 1.2:-64378689 None:intergenic
GAGGTTTCATCCAAGGTCCT+TGG 0.508636 1.2:+64379450 MS.gene07391:CDS
GGGACCCTGCTGCTAATTGG+AGG 0.508894 1.2:+64378684 MS.gene07391:CDS
TTCGGCTGTTCTATCATATG+TGG 0.516063 1.2:-64378264 None:intergenic
TATCTTGAAGGTCTATGATA+GGG 0.518400 1.2:-64378433 None:intergenic
TTCTTCAAAGGCCTTCACTT+CGG 0.520891 1.2:-64378282 None:intergenic
AGTACCTCAGCATGTAAAGA+TGG 0.523143 1.2:-64378467 None:intergenic
GTTCACAAGTGTTTACCATC+GGG 0.530662 1.2:+64379368 MS.gene07391:CDS
TACATATTCCAGATTCTAAG+AGG 0.531431 1.2:-64378325 None:intergenic
TGTTCTATCATATGTGGAAG+AGG 0.537060 1.2:-64378258 None:intergenic
AGATGCTTCACTTTCATGAT+CGG 0.538302 1.2:-64378396 None:intergenic
TCGAAGTGCATGTAAGGAGT+GGG 0.538796 1.2:+64378499 MS.gene07391:CDS
ACTACTGTGAAAGAAGTCTT+GGG 0.544052 1.2:+64379522 MS.gene07391:CDS
TGTTCACAAGTGTTTACCAT+CGG 0.545346 1.2:+64379367 MS.gene07391:CDS
GTTTGCAAAGAAGCTAGCTA+TGG 0.548364 1.2:-64379421 None:intergenic
CTTCTTTGCAAACTCAGTAG+AGG 0.552479 1.2:+64379431 MS.gene07391:CDS
GCAAGGAAGAGTTCCCATCA+AGG 0.565323 1.2:-64379563 None:intergenic
CATCTTGTTCATGAAACCTA+CGG 0.566748 1.2:-64378586 None:intergenic
CTCAGTAGAGGTTTCATCCA+AGG 0.567334 1.2:+64379443 MS.gene07391:CDS
TGATAGAACAGCCGAAGTGA+AGG 0.583274 1.2:+64378271 MS.gene07391:CDS
ATGAACAAGATGCTGATGCA+AGG 0.589480 1.2:+64378597 MS.gene07391:CDS
AGCTAGCTATGGAAACTCTA+GGG 0.590641 1.2:-64379410 None:intergenic
TCACAACCTGAAAGGGTGCA+AGG 0.591043 1.2:-64379580 None:intergenic
TTCGAAGTGCATGTAAGGAG+TGG 0.595798 1.2:+64378498 MS.gene07391:CDS
GTAGTATCCCTATAGATTGG+CGG 0.602342 1.2:-64379504 None:intergenic
ACAATTCCTTTATTGGACCA+AGG 0.603315 1.2:-64379467 None:intergenic
TAATCCATCTTTACATGCTG+AGG 0.617958 1.2:+64378463 MS.gene07391:CDS
AGATGATTAGTGGAATCCGT+AGG 0.632212 1.2:+64378570 MS.gene07391:CDS
GGACCCTGCTGCTAATTGGA+GGG 0.634253 1.2:+64378685 MS.gene07391:CDS
CGAAGTGCATGTAAGGAGTG+GGG 0.653285 1.2:+64378500 MS.gene07391:CDS
AGTCCCTCCAATTAGCAGCA+GGG 0.660661 1.2:-64378688 None:intergenic
TGGGAGAATGGAACATACGT+GGG 0.666519 1.2:-64378358 None:intergenic
AAGGAAACCATTCTATACAA+GGG 0.666588 1.2:+64378616 MS.gene07391:CDS
TTGGGAGAATGGAACATACG+TGG 0.674537 1.2:-64378359 None:intergenic
TATGCTTCGATAAAACCCGA+TGG 0.683714 1.2:-64379383 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTAATATTTGTTTTATTTTT+TGG + chr1.2:64378772-64378791 MS.gene07391:intron 10.0%
!!! ATTTGTTTTATTTTTTGGAT+CGG + chr1.2:64378777-64378796 MS.gene07391:intron 15.0%
!! AATTGTCATGAAATCAATAT+CGG - chr1.2:64379150-64379169 None:intergenic 20.0%
!!! TTTGTTTTATTTTTTGGATC+GGG + chr1.2:64378778-64378797 MS.gene07391:intron 20.0%
!!! TTTTTTCAAGTGATTAATCA+TGG + chr1.2:64378524-64378543 MS.gene07391:CDS 20.0%
! AAAGTTCGATATTTCTCTAA+TGG + chr1.2:64378650-64378669 MS.gene07391:CDS 25.0%
! AAGAAATTGTCCATAATCTA+AGG - chr1.2:64379066-64379085 None:intergenic 25.0%
! GATTAGTGTTTATATCTTGA+AGG - chr1.2:64378448-64378467 None:intergenic 25.0%
! TCCAATAAAGGAATTGTTAT+CGG + chr1.2:64379473-64379492 MS.gene07391:CDS 25.0%
! TTGATGAGATGATTATGTAT+TGG + chr1.2:64378930-64378949 MS.gene07391:intron 25.0%
!!! TTGGGACATTATTTTACAAA+AGG + chr1.2:64379540-64379559 MS.gene07391:CDS 25.0%
AACAAATATTACCTGCATAC+TGG - chr1.2:64378764-64378783 None:intergenic 30.0%
AAGGAAACCATTCTATACAA+GGG + chr1.2:64378616-64378635 MS.gene07391:CDS 30.0%
AAGTTCTTCATCAGAATCAA+TGG + chr1.2:64379198-64379217 MS.gene07391:intron 30.0%
AGTGATTAATCATGGTATTC+CGG + chr1.2:64378532-64378551 MS.gene07391:CDS 30.0%
AGTTCTTCATCAGAATCAAT+GGG + chr1.2:64379199-64379218 MS.gene07391:intron 30.0%
ATATCTTGAAGGTCTATGAT+AGG - chr1.2:64378437-64378456 None:intergenic 30.0%
CTTATGTACTTCACTTGTAA+AGG + chr1.2:64378883-64378902 MS.gene07391:intron 30.0%
TAATCATCTCATCAAAGACA+AGG - chr1.2:64378925-64378944 None:intergenic 30.0%
TACATATTCCAGATTCTAAG+AGG - chr1.2:64378328-64378347 None:intergenic 30.0%
TATCTTGAAGGTCTATGATA+GGG - chr1.2:64378436-64378455 None:intergenic 30.0%
TCAAAGAAAGTTAGAGCTTT+AGG + chr1.2:64378975-64378994 MS.gene07391:intron 30.0%
TCCGATAACAATTCCTTTAT+TGG - chr1.2:64379477-64379496 None:intergenic 30.0%
! ATTTTACAAAAGGCCTTGAT+GGG + chr1.2:64379550-64379569 MS.gene07391:CDS 30.0%
! GCCTTTGAAGAATCAAAATT+CGG + chr1.2:64378293-64378312 MS.gene07391:CDS 30.0%
! TATTTTACAAAAGGCCTTGA+TGG + chr1.2:64379549-64379568 MS.gene07391:CDS 30.0%
AAATATTACCTGCATACTGG+CGG - chr1.2:64378761-64378780 None:intergenic 35.0%
AAGAAAATCCGCCAATCTAT+AGG + chr1.2:64379496-64379515 MS.gene07391:CDS 35.0%
ACAATTCCTTTATTGGACCA+AGG - chr1.2:64379470-64379489 None:intergenic 35.0%
ACAGTAGTATCCCTATAGAT+TGG - chr1.2:64379510-64379529 None:intergenic 35.0%
ACTACTGTGAAAGAAGTCTT+GGG + chr1.2:64379522-64379541 MS.gene07391:CDS 35.0%
AGAAAATCCGCCAATCTATA+GGG + chr1.2:64379497-64379516 MS.gene07391:CDS 35.0%
AGAAGTTCCCATCGTTAATT+CGG - chr1.2:64379120-64379139 None:intergenic 35.0%
AGATGCTTCACTTTCATGAT+CGG - chr1.2:64378399-64378418 None:intergenic 35.0%
ATCTCATCCAAAACACTAAC+CGG - chr1.2:64378554-64378573 None:intergenic 35.0%
ATTCTTCTACAAGACCAGAT+TGG + chr1.2:64379167-64379186 MS.gene07391:intron 35.0%
CAAGGAAACCATTCTATACA+AGG + chr1.2:64378615-64378634 MS.gene07391:CDS 35.0%
CATCTTGTTCATGAAACCTA+CGG - chr1.2:64378589-64378608 None:intergenic 35.0%
TAATCCATCTTTACATGCTG+AGG + chr1.2:64378463-64378482 MS.gene07391:CDS 35.0%
TACTACTGTGAAAGAAGTCT+TGG + chr1.2:64379521-64379540 MS.gene07391:CDS 35.0%
TATGATAGGGACATTGAACT+TGG - chr1.2:64378423-64378442 None:intergenic 35.0%
TCAAGTTCAAATTGGGAGAA+TGG - chr1.2:64378373-64378392 None:intergenic 35.0%
TGTTCACAAGTGTTTACCAT+CGG + chr1.2:64379367-64379386 MS.gene07391:CDS 35.0%
TGTTCTATCATATGTGGAAG+AGG - chr1.2:64378261-64378280 None:intergenic 35.0%
! AATCTAAGGCCAAATCTCTA+AGG - chr1.2:64379052-64379071 None:intergenic 35.0%
! ATCGGAGTTCAAGTTCAAAT+TGG - chr1.2:64378381-64378400 None:intergenic 35.0%
! CTCTAATGGTAGCCTTTTTA+GGG + chr1.2:64378664-64378683 MS.gene07391:CDS 35.0%
! GGATCTCTTGTTGTAAACAT+CGG + chr1.2:64379242-64379261 MS.gene07391:intron 35.0%
! TCGGAGTTCAAGTTCAAATT+GGG - chr1.2:64378380-64378399 None:intergenic 35.0%
! TCTCTAATGGTAGCCTTTTT+AGG + chr1.2:64378663-64378682 MS.gene07391:CDS 35.0%
!! AGAGATTTGGCCTTAGATTA+TGG + chr1.2:64379053-64379072 MS.gene07391:intron 35.0%
!! GTTTTGGATGAGATGATTAG+TGG + chr1.2:64378560-64378579 MS.gene07391:CDS 35.0%
!!! GCCGAATTTTGATTCTTCAA+AGG - chr1.2:64378297-64378316 None:intergenic 35.0%
AAACATCGGTGATCTTATGC+AGG + chr1.2:64379256-64379275 MS.gene07391:intron 40.0%
AAGCTAGCTATGGAAACTCT+AGG - chr1.2:64379414-64379433 None:intergenic 40.0%
AGATGATTAGTGGAATCCGT+AGG + chr1.2:64378570-64378589 MS.gene07391:CDS 40.0%
AGCTAGCTATGGAAACTCTA+GGG - chr1.2:64379413-64379432 None:intergenic 40.0%
AGTACCTCAGCATGTAAAGA+TGG - chr1.2:64378470-64378489 None:intergenic 40.0%
ATGAACAAGATGCTGATGCA+AGG + chr1.2:64378597-64378616 MS.gene07391:CDS 40.0%
CTTCTTTGCAAACTCAGTAG+AGG + chr1.2:64379431-64379450 MS.gene07391:CDS 40.0%
CTTGCATCCCTTGTATAGAA+TGG - chr1.2:64378626-64378645 None:intergenic 40.0%
GAAGATTCGAAGTGCATGTA+AGG + chr1.2:64378493-64378512 MS.gene07391:CDS 40.0%
GAAGTTCCCATCGTTAATTC+GGG - chr1.2:64379119-64379138 None:intergenic 40.0%
GAATCAAAATTCGGCGTGAA+AGG + chr1.2:64378302-64378321 MS.gene07391:CDS 40.0%
GAGCTATTCTCAGAAGCTTT+AGG + chr1.2:64379011-64379030 MS.gene07391:intron 40.0%
GTAGTATCCCTATAGATTGG+CGG - chr1.2:64379507-64379526 None:intergenic 40.0%
GTTCACAAGTGTTTACCATC+GGG + chr1.2:64379368-64379387 MS.gene07391:CDS 40.0%
GTTTGCAAAGAAGCTAGCTA+TGG - chr1.2:64379424-64379443 None:intergenic 40.0%
TATGCTTCGATAAAACCCGA+TGG - chr1.2:64379386-64379405 None:intergenic 40.0%
TCCTTCTCACCTTAGAGATT+TGG + chr1.2:64379040-64379059 MS.gene07391:intron 40.0%
TTCGGCTGTTCTATCATATG+TGG - chr1.2:64378267-64378286 None:intergenic 40.0%
TTCTTCAAAGGCCTTCACTT+CGG - chr1.2:64378285-64378304 None:intergenic 40.0%
! TGGTATTCCGGTTAGTGTTT+TGG + chr1.2:64378544-64378563 MS.gene07391:CDS 40.0%
!! CGGGTTTTATCGAAGCATAT+AGG + chr1.2:64379387-64379406 MS.gene07391:CDS 40.0%
!! CTCTAAGGTGAGAAGGATTA+AGG - chr1.2:64379037-64379056 None:intergenic 40.0%
AAGAAATACCGCCAGTATGC+AGG + chr1.2:64378750-64378769 MS.gene07391:intron 45.0%
ACAACAAGAGATCCATGCAG+AGG - chr1.2:64379236-64379255 None:intergenic 45.0%
CAAGGTCCTTGGTCCAATAA+AGG + chr1.2:64379461-64379480 MS.gene07391:CDS 45.0%
CATACTGGCGGTATTTCTTC+TGG - chr1.2:64378749-64378768 None:intergenic 45.0%
CCTGAACCCGAATTAACGAT+GGG + chr1.2:64379110-64379129 MS.gene07391:intron 45.0%
CTCAGTAGAGGTTTCATCCA+AGG + chr1.2:64379443-64379462 MS.gene07391:CDS 45.0%
CTTCTACAAGACCAGATTGG+TGG + chr1.2:64379170-64379189 MS.gene07391:intron 45.0%
CTTTATTGGACCAAGGACCT+TGG - chr1.2:64379463-64379482 None:intergenic 45.0%
GAACTTGAAGACCACCAATC+TGG - chr1.2:64379184-64379203 None:intergenic 45.0%
GGCGGTATTTCTTCTGGATT+GGG - chr1.2:64378743-64378762 None:intergenic 45.0%
GTGAAAGGCCTCTTAGAATC+TGG + chr1.2:64378317-64378336 MS.gene07391:CDS 45.0%
GTTGATGTTACTCCTCTGCA+TGG + chr1.2:64379221-64379240 MS.gene07391:intron 45.0%
TCGAAGTGCATGTAAGGAGT+GGG + chr1.2:64378499-64378518 MS.gene07391:CDS 45.0%
TGATAGAACAGCCGAAGTGA+AGG + chr1.2:64378271-64378290 MS.gene07391:CDS 45.0%
TGGCGGTATTTCTTCTGGAT+TGG - chr1.2:64378744-64378763 None:intergenic 45.0%
TGGGAGAATGGAACATACGT+GGG - chr1.2:64378361-64378380 None:intergenic 45.0%
TTCGAAGTGCATGTAAGGAG+TGG + chr1.2:64378498-64378517 MS.gene07391:CDS 45.0%
TTGGGAGAATGGAACATACG+TGG - chr1.2:64378362-64378381 None:intergenic 45.0%
! CCATCGTTAATTCGGGTTCA+GGG - chr1.2:64379112-64379131 None:intergenic 45.0%
! GGTATTTCTTCTGGATTGGG+AGG - chr1.2:64378740-64378759 None:intergenic 45.0%
! TCTTCTGGATTGGGAGGATT+GGG - chr1.2:64378734-64378753 None:intergenic 45.0%
! TTCTTCTGGATTGGGAGGAT+TGG - chr1.2:64378735-64378754 None:intergenic 45.0%
!! GCCAAATCTCTAAGGTGAGA+AGG - chr1.2:64379044-64379063 None:intergenic 45.0%
ACTCTTCCTTGCACCCTTTC+AGG + chr1.2:64379574-64379593 MS.gene07391:CDS 50.0%
AGTCCCTCCAATTAGCAGCA+GGG - chr1.2:64378691-64378710 None:intergenic 50.0%
CCCTGAACCCGAATTAACGA+TGG + chr1.2:64379109-64379128 MS.gene07391:intron 50.0%
CGAAGTGCATGTAAGGAGTG+GGG + chr1.2:64378500-64378519 MS.gene07391:CDS 50.0%
GAGGTTTCATCCAAGGTCCT+TGG + chr1.2:64379450-64379469 MS.gene07391:CDS 50.0%
GCAAGGAAGAGTTCCCATCA+AGG - chr1.2:64379566-64379585 None:intergenic 50.0%
TAGCAGCAGGGTCCCTAAAA+AGG - chr1.2:64378679-64378698 None:intergenic 50.0%
TTAGGGACCCTGCTGCTAAT+TGG + chr1.2:64378681-64378700 MS.gene07391:CDS 50.0%
! CCCATCGTTAATTCGGGTTC+AGG - chr1.2:64379113-64379132 None:intergenic 50.0%
! TCACAACCTGAAAGGGTGCA+AGG - chr1.2:64379583-64379602 None:intergenic 50.0%
GAGTCCCTCCAATTAGCAGC+AGG - chr1.2:64378692-64378711 None:intergenic 55.0%
GGACCCTGCTGCTAATTGGA+GGG + chr1.2:64378685-64378704 MS.gene07391:CDS 55.0%
! AATTCGGGTTCAGGGCATGC+AGG - chr1.2:64379104-64379123 None:intergenic 55.0%
GGGACCCTGCTGCTAATTGG+AGG + chr1.2:64378684-64378703 MS.gene07391:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 64378233 64379602 64378233 ID=MS.gene07391
chr1.2 mRNA 64378233 64379602 64378233 ID=MS.gene07391.t1;Parent=MS.gene07391
chr1.2 exon 64378233 64378724 64378233 ID=MS.gene07391.t1.exon1;Parent=MS.gene07391.t1
chr1.2 CDS 64378233 64378724 64378233 ID=cds.MS.gene07391.t1;Parent=MS.gene07391.t1
chr1.2 exon 64379351 64379602 64379351 ID=MS.gene07391.t1.exon2;Parent=MS.gene07391.t1
chr1.2 CDS 64379351 64379602 64379351 ID=cds.MS.gene07391.t1;Parent=MS.gene07391.t1
Gene Sequence

>MS.gene07391

ATGGAGTTAGAGGCAAGTAACGTTTCCTCTTCCACATATGATAGAACAGCCGAAGTGAAGGCCTTTGAAGAATCAAAATTCGGCGTGAAAGGCCTCTTAGAATCTGGAATATGTACGACAAAGATCCCACGTATGTTCCATTCTCCCAATTTGAACTTGAACTCCGATCATGAAAGTGAAGCATCTTCCAAGTTCAATGTCCCTATCATAGACCTTCAAGATATAAACACTAATCCATCTTTACATGCTGAGGTACTTGAGAAGATTCGAAGTGCATGTAAGGAGTGGGGATTTTTTCAAGTGATTAATCATGGTATTCCGGTTAGTGTTTTGGATGAGATGATTAGTGGAATCCGTAGGTTTCATGAACAAGATGCTGATGCAAGGAAACCATTCTATACAAGGGATGCAAGTAAGAAAGTTCGATATTTCTCTAATGGTAGCCTTTTTAGGGACCCTGCTGCTAATTGGAGGGACTCGATTACTTTTTTTGTAAGTCCCAATCCTCCCAATCCAGAAGAAATACCGCCAGTATGCAGGTAATATTTGTTTTATTTTTTGGATCGGGCAAAGTTATTTCGCTTAGAGATCAATCACTGAGTTTACAAGTTTTTGCTTAAACTACACTTACAAGTTTTTCTTAAATTTCACTTATGTACTTCACTTGTAAAGGTTATCAATGTTTTTTTCCTTGTCTTTGATGAGATGATTATGTATTGGCAGAGACATTGTGATTGAATATTCAAAGAAAGTTAGAGCTTTAGGCTTGACAATCTTTGAGCTATTCTCAGAAGCTTTAGGCCTTAATCCTTCTCACCTTAGAGATTTGGCCTTAGATTATGGACAATTTCTTTTGTGTCACTACTATCCTGCATGCCCTGAACCCGAATTAACGATGGGAACTTCTAAGCACACCGATATTGATTTCATGACAATTCTTCTACAAGACCAGATTGGTGGTCTTCAAGTTCTTCATCAGAATCAATGGGTTGATGTTACTCCTCTGCATGGATCTCTTGTTGTAAACATCGGTGATCTTATGCAGGTAAGTGATGTTAAATGTAAACTCTTCAATTATTTCTGCATTGTCATGTGACTTTTATCGATTTTCTTCGCAGCTTGTAACAAATGACATGTTCACAAGTGTTTACCATCGGGTTTTATCGAAGCATATAGGCCCTAGAGTTTCCATAGCTAGCTTCTTTGCAAACTCAGTAGAGGTTTCATCCAAGGTCCTTGGTCCAATAAAGGAATTGTTATCGGAAGAAAATCCGCCAATCTATAGGGATACTACTGTGAAAGAAGTCTTGGGACATTATTTTACAAAAGGCCTTGATGGGAACTCTTCCTTGCACCCTTTCAGGTTGTGA
Protein sequence

>MS.gene07391.t1

MELEASNVSSSTYDRTAEVKAFEESKFGVKGLLESGICTTKIPRMFHSPNLNLNSDHESEASSKFNVPIIDLQDINTNPSLHAEVLEKIRSACKEWGFFQVINHGIPVSVLDEMISGIRRFHEQDADARKPFYTRDASKKVRYFSNGSLFRDPAANWRDSITFFLVTNDMFTSVYHRVLSKHIGPRVSIASFFANSVEVSSKVLGPIKELLSEENPPIYRDTTVKEVLGHYFTKGLDGNSSLHPFRL