AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene073984

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073984.t1 MTR_0036s0070 89.552 335 34 1 1 335 93 426 0.0 613
MS.gene073984.t1 MTR_0036s0050 74.783 345 77 3 1 336 93 436 0.0 517
MS.gene073984.t1 MTR_0036s0110 60.345 348 125 4 1 336 97 443 5.93e-143 411
MS.gene073984.t1 MTR_1g060750 55.394 343 127 6 1 336 54 377 3.29e-120 351
MS.gene073984.t1 MTR_8g096990 45.533 347 173 8 1 335 89 431 1.74e-97 295
MS.gene073984.t1 MTR_5g080890 43.840 349 178 7 1 335 89 433 3.54e-93 284
MS.gene073984.t1 MTR_5g080960 43.553 349 179 7 1 335 89 433 1.28e-92 283
MS.gene073984.t1 MTR_8g038280 41.311 351 186 9 1 335 89 435 3.21e-87 269
MS.gene073984.t1 MTR_5g044770 41.040 346 189 8 1 335 89 430 1.89e-85 264
MS.gene073984.t1 MTR_8g038250 41.595 351 165 8 1 335 89 415 7.91e-83 257
MS.gene073984.t1 MTR_6g090290 40.057 352 190 8 1 335 89 436 9.08e-82 255
MS.gene073984.t1 MTR_5g008110 41.143 350 189 10 1 336 70 416 2.45e-77 243
MS.gene073984.t1 MTR_3g097150 40.974 349 187 6 1 335 105 448 9.94e-77 242
MS.gene073984.t1 MTR_2g059490 40.456 351 190 9 1 334 61 409 8.04e-75 237
MS.gene073984.t1 MTR_4g109760 41.888 339 176 9 11 335 67 398 3.18e-74 234
MS.gene073984.t1 MTR_2g059460 38.352 352 193 8 1 335 62 406 1.59e-73 233
MS.gene073984.t1 MTR_5g008070 40.290 345 192 7 1 334 84 425 3.97e-73 233
MS.gene073984.t1 MTR_8g038310 39.535 344 179 8 1 335 90 413 4.92e-73 232
MS.gene073984.t1 MTR_3g028120 38.286 350 198 7 1 335 92 438 6.41e-73 232
MS.gene073984.t1 MTR_5g008150 39.031 351 196 10 1 336 66 413 3.77e-71 227
MS.gene073984.t1 MTR_0024s0040 38.286 350 196 8 1 335 66 410 2.94e-68 219
MS.gene073984.t1 MTR_2g067830 37.283 346 199 9 5 335 64 406 1.41e-66 215
MS.gene073984.t1 MTR_5g038360 37.216 352 198 10 1 335 91 436 7.46e-66 214
MS.gene073984.t1 MTR_2g105300 36.578 339 196 10 9 332 47 381 2.86e-65 211
MS.gene073984.t1 MTR_5g044570 37.391 345 183 9 1 336 92 412 5.28e-62 203
MS.gene073984.t1 MTR_6g088760 37.342 316 178 8 5 303 95 407 5.93e-61 203
MS.gene073984.t1 MTR_7g117430 35.817 349 198 11 2 333 145 484 5.60e-54 184
MS.gene073984.t1 MTR_4g045857 48.558 208 97 5 1 200 61 266 1.57e-52 175
MS.gene073984.t1 MTR_1g115210 33.524 349 205 10 1 333 149 486 2.37e-48 169
MS.gene073984.t1 MTR_3g046300 34.605 367 202 12 1 335 100 460 3.50e-48 168
MS.gene073984.t1 MTR_5g008090 36.700 297 172 9 53 335 20 314 5.92e-48 164
MS.gene073984.t1 MTR_5g091130 33.973 365 206 13 1 334 194 554 3.77e-47 167
MS.gene073984.t1 MTR_4g007740 33.524 349 210 8 3 334 135 478 6.28e-46 162
MS.gene073984.t1 MTR_1g039270 31.714 350 211 12 1 333 131 469 1.30e-45 161
MS.gene073984.t1 MTR_1g111350 32.471 348 204 9 2 333 135 467 2.01e-44 158
MS.gene073984.t1 MTR_6g085010 32.653 343 209 10 2 333 160 491 5.89e-44 157
MS.gene073984.t1 MTR_2g028190 30.682 352 220 11 1 333 105 451 9.31e-35 132
MS.gene073984.t1 MTR_3g053710 31.250 352 210 12 5 333 124 466 1.52e-34 132
MS.gene073984.t1 MTR_5g016260 31.755 359 203 18 2 335 92 433 2.18e-33 130
MS.gene073984.t1 MTR_5g016260 31.755 359 203 18 2 335 92 433 3.44e-33 129
MS.gene073984.t1 MTR_3g053980 32.951 349 202 13 5 333 149 485 1.20e-31 124
MS.gene073984.t1 MTR_2g055840 26.870 361 229 9 3 333 18 373 2.39e-31 121
MS.gene073984.t1 MTR_8g103880 27.761 335 218 9 1 321 119 443 3.12e-31 122
MS.gene073984.t1 MTR_4g095270 29.132 357 215 15 2 335 84 425 6.14e-29 117
MS.gene073984.t1 MTR_2g084550 30.728 371 206 19 2 334 81 438 8.55e-29 116
MS.gene073984.t1 MTR_4g095270 29.132 357 215 16 2 335 84 425 1.03e-28 117
MS.gene073984.t1 MTR_4g056420 29.076 368 215 17 2 334 81 437 1.18e-28 115
MS.gene073984.t1 MTR_2g070430 30.769 377 210 19 1 335 90 457 1.02e-27 112
MS.gene073984.t1 MTR_3g068000 28.831 385 203 15 1 333 76 441 1.51e-27 112
MS.gene073984.t1 MTR_3g065160 27.838 370 206 15 5 335 81 428 5.86e-27 110
MS.gene073984.t1 MTR_7g102750 29.301 372 209 17 2 334 85 441 6.40e-26 108
MS.gene073984.t1 MTR_1g061940 27.926 376 215 16 2 336 87 447 1.12e-25 107
MS.gene073984.t1 MTR_7g105850 30.964 394 194 22 1 335 93 467 1.70e-25 106
MS.gene073984.t1 MTR_1g037630 30.659 349 213 11 1 334 95 429 2.18e-25 105
MS.gene073984.t1 MTR_3g014290 30.659 349 213 11 1 334 83 417 2.34e-25 105
MS.gene073984.t1 MTR_7g080230 27.869 366 226 12 2 336 75 433 6.73e-25 105
MS.gene073984.t1 MTR_4g100990 28.093 388 200 16 17 334 93 471 1.39e-24 104
MS.gene073984.t1 MTR_1g078240 27.614 373 219 17 2 336 73 432 1.24e-23 101
MS.gene073984.t1 MTR_7g102750 31.269 323 172 17 2 289 85 392 2.22e-23 100
MS.gene073984.t1 MTR_7g021510 25.661 378 217 15 2 334 76 434 8.91e-23 99.0
MS.gene073984.t1 MTR_8g039540 28.161 348 224 9 1 334 99 434 1.76e-22 97.8
MS.gene073984.t1 MTR_5g061690 28.152 341 210 13 3 321 106 433 4.36e-22 97.4
MS.gene073984.t1 MTR_1g078240 27.540 374 219 18 2 336 73 433 5.30e-22 96.7
MS.gene073984.t1 MTR_5g012490 25.879 398 212 17 8 334 85 470 5.91e-21 93.6
MS.gene073984.t1 MTR_3g087780 26.039 361 223 14 3 333 104 450 1.29e-20 92.8
MS.gene073984.t1 MTR_5g077850 28.378 370 208 12 5 335 88 439 2.39e-20 91.7
MS.gene073984.t1 MTR_1g078240 31.939 263 142 12 2 237 73 325 2.87e-20 90.9
MS.gene073984.t1 MTR_7g062360 26.400 375 223 14 1 333 62 425 3.87e-20 90.9
MS.gene073984.t1 MTR_8g075010 26.039 361 221 14 3 333 105 449 5.41e-20 91.3
MS.gene073984.t1 MTR_1g029810 27.273 363 217 17 5 335 64 411 7.80e-20 90.1
MS.gene073984.t1 MTR_1g029810 26.975 367 221 17 1 335 60 411 9.79e-20 90.1
MS.gene073984.t1 MTR_5g022880 25.978 358 225 15 3 333 117 461 5.22e-18 85.1
MS.gene073984.t1 MTR_7g080230 26.531 343 217 12 22 336 28 363 6.47e-17 81.3
MS.gene073984.t1 MTR_6g084710 24.862 362 234 15 3 333 196 550 1.59e-16 80.9
MS.gene073984.t1 MTR_6g084710 27.907 258 167 10 3 248 196 446 1.85e-16 80.5
MS.gene073984.t1 MTR_4g008110 39.130 115 44 3 70 181 46 137 2.51e-16 75.1
MS.gene073984.t1 MTR_0945s0010 31.707 123 82 2 214 334 1 123 4.51e-15 72.4
MS.gene073984.t1 MTR_5g077920 25.464 377 214 15 5 336 75 429 1.91e-14 74.3
MS.gene073984.t1 MTR_5g061690 26.846 298 194 11 36 321 9 294 2.27e-14 73.6
MS.gene073984.t1 MTR_3g100500 28.652 356 213 13 1 333 100 437 2.69e-14 73.9
MS.gene073984.t1 MTR_3g006820 24.932 365 223 17 6 335 66 414 4.34e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073984.t1 AT5G33340 45.115 348 175 6 1 335 92 436 2.43e-88 272
MS.gene073984.t1 AT1G64830 44.669 347 176 7 1 335 88 430 6.54e-87 268
MS.gene073984.t1 AT1G31450 45.304 362 168 11 1 336 87 444 2.15e-85 265
MS.gene073984.t1 AT2G35615 43.681 364 173 12 1 336 87 446 5.27e-82 256
MS.gene073984.t1 AT2G28010 39.943 348 175 11 1 335 67 393 1.06e-73 233
MS.gene073984.t1 AT2G28030 40.115 349 173 12 1 335 63 389 3.43e-71 226
MS.gene073984.t1 AT2G03200 39.944 358 186 9 1 335 109 460 7.07e-71 228
MS.gene073984.t1 AT2G28040 39.306 346 177 11 1 334 58 382 3.47e-69 221
MS.gene073984.t1 AT2G28040 39.306 346 177 11 1 334 67 391 4.58e-69 221
MS.gene073984.t1 AT2G28220 39.714 350 177 14 1 335 69 399 3.44e-66 214
MS.gene073984.t1 AT2G28225 39.316 351 177 13 1 335 69 399 3.69e-64 209
MS.gene073984.t1 AT1G01300 34.659 352 200 12 2 334 145 485 4.11e-48 169
MS.gene073984.t1 AT3G61820 35.933 359 192 12 1 333 137 483 5.12e-47 166
MS.gene073984.t1 AT4G30030 34.746 354 192 16 3 333 82 419 5.42e-46 162
MS.gene073984.t1 AT3G18490 31.700 347 211 10 2 333 165 500 3.22e-45 161
MS.gene073984.t1 AT2G42980 31.759 381 196 12 1 334 162 525 1.19e-42 155
MS.gene073984.t1 AT2G23945 32.249 369 192 17 5 335 102 450 1.83e-42 153
MS.gene073984.t1 AT3G59080 32.075 371 206 15 1 334 172 533 1.98e-42 154
MS.gene073984.t1 AT3G25700 29.650 371 218 12 1 334 86 450 2.54e-42 152
MS.gene073984.t1 AT1G25510 33.043 345 206 10 2 333 151 483 5.72e-42 152
MS.gene073984.t1 AT3G20015 31.304 345 218 7 1 333 133 470 3.68e-41 150
MS.gene073984.t1 AT5G10770 33.046 348 212 10 1 334 134 474 1.25e-39 145
MS.gene073984.t1 AT4G30040 31.755 359 196 14 1 328 87 427 2.94e-39 144
MS.gene073984.t1 AT5G36260 30.137 365 216 13 2 336 81 436 4.85e-34 130
MS.gene073984.t1 AT1G79720 32.670 352 186 14 15 333 149 482 3.21e-33 128
MS.gene073984.t1 AT5G43100 29.014 355 215 15 2 334 79 418 1.21e-32 127
MS.gene073984.t1 AT5G43100 29.014 355 215 15 2 334 79 418 5.02e-32 126
MS.gene073984.t1 AT3G50050 30.168 358 211 16 2 335 96 438 7.78e-32 125
MS.gene073984.t1 AT3G12700 29.167 360 221 12 2 334 109 461 2.27e-31 123
MS.gene073984.t1 AT3G59080 30.411 365 184 14 1 334 172 497 7.42e-31 122
MS.gene073984.t1 AT5G10760 33.819 343 205 11 1 333 134 464 2.26e-30 120
MS.gene073984.t1 AT3G54400 30.484 351 212 15 1 334 90 425 4.69e-30 119
MS.gene073984.t1 AT1G66180 28.226 372 213 14 1 335 74 428 5.51e-30 119
MS.gene073984.t1 AT4G12920 29.598 348 208 16 2 333 61 387 1.46e-29 117
MS.gene073984.t1 AT3G02740 28.926 363 218 15 2 334 13 365 1.24e-28 114
MS.gene073984.t1 AT3G02740 28.926 363 218 15 2 334 88 440 3.10e-28 114
MS.gene073984.t1 AT5G37540 28.342 374 210 15 1 333 82 438 8.42e-27 110
MS.gene073984.t1 AT1G08210 30.685 365 211 18 2 334 87 441 2.87e-26 109
MS.gene073984.t1 AT1G08210 30.685 365 211 18 2 334 121 475 3.19e-26 109
MS.gene073984.t1 AT1G08210 30.685 365 211 18 2 334 135 489 4.42e-26 108
MS.gene073984.t1 AT2G36670 27.446 368 224 13 2 336 37 394 5.68e-26 107
MS.gene073984.t1 AT2G36670 27.446 368 224 13 2 336 37 394 5.68e-26 107
MS.gene073984.t1 AT2G36670 28.804 368 219 16 2 336 108 465 8.96e-26 107
MS.gene073984.t1 AT2G36670 28.804 368 219 16 2 336 103 460 9.78e-26 107
MS.gene073984.t1 AT5G22850 29.032 372 215 17 2 336 84 443 9.91e-26 107
MS.gene073984.t1 AT5G45120 27.901 405 209 17 1 333 85 478 6.02e-25 105
MS.gene073984.t1 AT1G09750 26.271 354 229 11 1 333 106 448 7.10e-25 104
MS.gene073984.t1 AT5G02190 29.032 372 207 18 8 333 82 442 8.23e-25 104
MS.gene073984.t1 AT2G39710 28.075 374 206 18 3 329 69 426 1.71e-23 100
MS.gene073984.t1 AT3G52500 29.703 404 187 20 1 334 92 468 4.47e-23 99.8
MS.gene073984.t1 AT1G05840 25.926 378 218 15 2 336 83 441 8.18e-23 99.4
MS.gene073984.t1 AT1G65240 25.414 362 234 12 2 336 77 429 8.38e-23 99.0
MS.gene073984.t1 AT1G08210 31.385 325 182 17 2 295 87 401 1.00e-22 99.0
MS.gene073984.t1 AT1G49050 24.079 353 229 16 17 335 50 397 4.06e-21 93.6
MS.gene073984.t1 AT5G24820 28.870 239 150 7 106 333 170 399 2.18e-20 91.7
MS.gene073984.t1 AT1G49050 24.079 353 229 16 17 335 223 570 2.32e-20 92.4
MS.gene073984.t1 AT5G07030 26.722 363 213 15 1 334 117 455 3.43e-20 91.3
MS.gene073984.t1 AT3G51360 26.554 354 226 14 3 333 9 351 3.21e-19 88.2
MS.gene073984.t1 AT2G17760 25.070 359 224 15 3 333 108 449 4.16e-19 88.6
MS.gene073984.t1 AT3G51360 26.554 354 226 14 3 333 103 445 5.75e-19 88.2
MS.gene073984.t1 AT3G51360 26.554 354 226 14 3 333 93 435 5.91e-19 87.8
MS.gene073984.t1 AT3G51350 24.490 343 223 12 3 321 106 436 4.51e-18 85.5
MS.gene073984.t1 AT5G10080 22.802 364 240 12 3 336 104 456 1.62e-17 84.0
MS.gene073984.t1 AT4G16563 26.434 401 203 16 17 334 101 492 2.57e-17 83.2
MS.gene073984.t1 AT3G51360 27.679 336 209 14 3 315 103 427 1.33e-16 80.9
MS.gene073984.t1 AT3G51360 27.679 336 209 14 3 315 93 417 1.47e-16 80.5
MS.gene073984.t1 AT3G42550 24.719 356 197 11 3 334 82 390 2.57e-16 79.7
MS.gene073984.t1 AT3G25700 36.000 125 66 5 1 112 86 209 3.69e-14 72.8
MS.gene073984.t1 AT3G51340 22.286 350 244 10 3 333 107 447 3.87e-14 73.6
MS.gene073984.t1 AT3G51360 25.407 307 204 12 41 333 14 309 2.30e-13 70.5
MS.gene073984.t1 AT3G51360 25.407 307 204 12 41 333 14 309 2.30e-13 70.5

Find 72 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACGGGGAGTGACCTTATT+TGG 0.099816 2.1:+30191734 MS.gene073984:CDS
GTAAAATGAAACACAAAATT+AGG 0.174509 2.1:-30192480 None:intergenic
ACCAATGATCTTTCCATCTT+TGG 0.184135 2.1:+30192585 MS.gene073984:CDS
TTGCTATTTGAGTTTAAATT+TGG 0.187752 2.1:-30192147 None:intergenic
CTCTCACACCTCTGGAGATT+TGG 0.211933 2.1:+30191927 MS.gene073984:CDS
CAACCAAGTTCTGAGGTTTA+AGG 0.223072 2.1:-30192518 None:intergenic
TCAAATAGCAAAATGAAATT+TGG 0.248015 2.1:+30192159 MS.gene073984:CDS
GGAGATGAAGCAATTATAAA+AGG 0.259280 2.1:+30192180 MS.gene073984:CDS
GTAATTGGAAACTTAGTTCT+TGG 0.275900 2.1:-30192685 None:intergenic
AACAAAATCTTCCACTTTCA+TGG 0.282987 2.1:+30191813 MS.gene073984:CDS
CAACTAGACCCGTAGCTTTA+CGG 0.294559 2.1:-30192044 None:intergenic
GCTTCCTTGAAGGGAGTTAT+TGG 0.296078 2.1:+30192387 MS.gene073984:CDS
GGTAACATAATCATTGATTC+AGG 0.326198 2.1:+30192318 MS.gene073984:CDS
AACCAAGTTCTGAGGTTTAA+GGG 0.361825 2.1:-30192517 None:intergenic
TCTCACACCTCTGGAGATTT+GGG 0.368406 2.1:+30191928 MS.gene073984:CDS
TGCGAGTATAATTACACTTA+TGG 0.384233 2.1:+30191898 MS.gene073984:CDS
TCCAAAGATGGAAAGATCAT+TGG 0.384929 2.1:-30192586 None:intergenic
GGGAACATTGTACCCAAATA+AGG 0.386073 2.1:-30191747 None:intergenic
CACGGGGAGTGACCTTATTT+GGG 0.389068 2.1:+30191735 MS.gene073984:CDS
ATTTGGAGGAATCAAACAAT+AGG 0.396296 2.1:-30192130 None:intergenic
CAGAACTTGGTTGTTGATCA+TGG 0.399287 2.1:+30192528 MS.gene073984:CDS
TTGTGCTCTATTTCCAAAGA+TGG 0.401939 2.1:-30192598 None:intergenic
TAAAATGAAACACAAAATTA+GGG 0.403931 2.1:-30192479 None:intergenic
GGTACTGAATACATTAACTT+TGG 0.410941 2.1:+30191949 MS.gene073984:CDS
TACCCTTAAACCTCAGAACT+TGG 0.428511 2.1:+30192515 MS.gene073984:CDS
CGGTGATGCCTTCGAGATTG+AGG 0.452184 2.1:-30192260 None:intergenic
CAACAACTCGTATTGCTTGT+TGG 0.453868 2.1:+30192551 MS.gene073984:CDS
AACACCAATAACTCCCTTCA+AGG 0.454045 2.1:-30192391 None:intergenic
CTATTTGAGTTTAAATTTGG+AGG 0.456554 2.1:-30192144 None:intergenic
GATTTGAAGATTTGATTATG+AGG 0.456931 2.1:-30192224 None:intergenic
TGATTTGGTAGCTTCCTTGA+AGG 0.464165 2.1:+30192377 MS.gene073984:CDS
TACCTCATGAAAATCTATAT+AGG 0.467318 2.1:+30191679 None:intergenic
ACAAAATCTTCCACTTTCAT+GGG 0.473834 2.1:+30191814 MS.gene073984:CDS
GCTACGGGTCTAGTTGGTCT+TGG 0.478890 2.1:+30192051 MS.gene073984:CDS
GAACTTTGTTATATCTCCTA+AGG 0.488128 2.1:-30192457 None:intergenic
ATCAATGATTATGTTACCAT+CGG 0.500077 2.1:-30192313 None:intergenic
GGTGATAACTCTCACACCTC+TGG 0.503324 2.1:+30191919 MS.gene073984:CDS
TCACAACTAGGTGATTCAAT+TGG 0.503436 2.1:+30192096 MS.gene073984:CDS
ACATGACAAACCCATGAAAG+TGG 0.505680 2.1:-30191824 None:intergenic
TAAAAGAAGCCGTAAAGCTA+CGG 0.507090 2.1:+30192035 MS.gene073984:CDS
GCAACAGCAAGCCTTTCAAC+AGG 0.507502 2.1:-30191709 None:intergenic
GATTTGGTAGCTTCCTTGAA+GGG 0.510053 2.1:+30192378 MS.gene073984:CDS
CGTAAAGCTACGGGTCTAGT+TGG 0.511569 2.1:+30192045 MS.gene073984:CDS
GGTCTAGTTGGTCTTGGAGC+AGG 0.513423 2.1:+30192057 MS.gene073984:CDS
GAAGCAATTATAAAAGGTAA+TGG 0.516167 2.1:+30192186 MS.gene073984:CDS
ATTTGAAGATTTGATTATGA+GGG 0.518599 2.1:-30192223 None:intergenic
CTATCACTAGTTTCACAACT+AGG 0.533914 2.1:+30192084 MS.gene073984:CDS
TAGTTCTTGGAACAATCAGT+TGG 0.541167 2.1:-30192672 None:intergenic
AGGCTTGCTGTTGCAGACAC+GGG 0.541635 2.1:+30191718 MS.gene073984:CDS
AAAAGAAGCCGTAAAGCTAC+GGG 0.541737 2.1:+30192036 MS.gene073984:CDS
GAAGGGAGTTATTGGTGTTG+AGG 0.543742 2.1:+30192395 MS.gene073984:CDS
GGAACAATCAGTTGGAGCAA+AGG 0.550575 2.1:-30192664 None:intergenic
AAGGCTTGCTGTTGCAGACA+CGG 0.550762 2.1:+30191717 MS.gene073984:CDS
AGTTGTGAAACTAGTGATAG+TGG 0.555072 2.1:-30192081 None:intergenic
TCATAATAAGGAGTGTCTTG+TGG 0.564393 2.1:-30191787 None:intergenic
TTAGTAGGGTACGATCTCAA+AGG 0.581764 2.1:+30192630 MS.gene073984:CDS
CAGCAAGCCTTTCAACAGGT+GGG 0.584660 2.1:-30191705 None:intergenic
CCTTGCTCATTACTACCACA+TGG 0.585936 2.1:+30191856 MS.gene073984:CDS
TAGGCACCCCACCTGTTGAA+AGG 0.592550 2.1:+30191698 MS.gene073984:CDS
ACAGCAAGCCTTTCAACAGG+TGG 0.594361 2.1:-30191706 None:intergenic
TCATTACTACCACATGGATG+TGG 0.603546 2.1:+30191862 MS.gene073984:CDS
AGCAAGCCTTTCAACAGGTG+GGG 0.603962 2.1:-30191704 None:intergenic
GTAACATAATCATTGATTCA+GGG 0.608837 2.1:+30192319 MS.gene073984:CDS
ATGGAAAGATCATTGGTTGG+TGG 0.612301 2.1:-30192579 None:intergenic
TGATCAACAACCAAGTTCTG+AGG 0.624623 2.1:-30192525 None:intergenic
AAGATGGAAAGATCATTGGT+TGG 0.638032 2.1:-30192582 None:intergenic
GGCTTGCTGTTGCAGACACG+GGG 0.645457 2.1:+30191719 MS.gene073984:CDS
CCATGTGGTAGTAATGAGCA+AGG 0.648440 2.1:-30191856 None:intergenic
AATCTCGAAGGCATCACCGT+CGG 0.652587 2.1:+30192264 MS.gene073984:CDS
TTCAGTACCCAAATCTCCAG+AGG 0.667883 2.1:-30191935 None:intergenic
GTTCAGACAGGTCAAACCGA+TGG 0.685598 2.1:+30192297 MS.gene073984:CDS
TTTGAGTTTCCACATCCATG+TGG 0.688732 2.1:-30191871 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAATGAAACACAAAATTA+GGG - chr2.1:30192482-30192501 None:intergenic 15.0%
!!! CACAATTTAATTTTTTAGTA+GGG + chr2.1:30192616-30192635 MS.gene073984:CDS 15.0%
!!! TAATTTTGTGTTTCATTTTA+CGG + chr2.1:30192482-30192501 MS.gene073984:CDS 15.0%
!! ATTTGAAGATTTGATTATGA+GGG - chr2.1:30192226-30192245 None:intergenic 20.0%
!! GTAAAATGAAACACAAAATT+AGG - chr2.1:30192483-30192502 None:intergenic 20.0%
!! TCAAATAGCAAAATGAAATT+TGG + chr2.1:30192159-30192178 MS.gene073984:CDS 20.0%
!!! AATTTTGTGTTTCATTTTAC+GGG + chr2.1:30192483-30192502 MS.gene073984:CDS 20.0%
!!! ACAAAACTTTTACAATGATT+TGG + chr2.1:30192362-30192381 MS.gene073984:CDS 20.0%
!!! ACATTTCCTAAATCAATTTT+TGG + chr2.1:30191985-30192004 MS.gene073984:CDS 20.0%
!!! GCACAATTTAATTTTTTAGT+AGG + chr2.1:30192615-30192634 MS.gene073984:CDS 20.0%
!!! TTGCTATTTGAGTTTAAATT+TGG - chr2.1:30192150-30192169 None:intergenic 20.0%
! ATCAATGATTATGTTACCAT+CGG - chr2.1:30192316-30192335 None:intergenic 25.0%
! GAAGCAATTATAAAAGGTAA+TGG + chr2.1:30192186-30192205 MS.gene073984:CDS 25.0%
! GATTTGAAGATTTGATTATG+AGG - chr2.1:30192227-30192246 None:intergenic 25.0%
!! CTATTTGAGTTTAAATTTGG+AGG - chr2.1:30192147-30192166 None:intergenic 25.0%
!! GATTTTGTTGGATCATAATA+AGG - chr2.1:30191802-30191821 None:intergenic 25.0%
!!! GTAACATAATCATTGATTCA+GGG + chr2.1:30192319-30192338 MS.gene073984:CDS 25.0%
AACAAAATCTTCCACTTTCA+TGG + chr2.1:30191813-30191832 MS.gene073984:CDS 30.0%
ACAAAATCTTCCACTTTCAT+GGG + chr2.1:30191814-30191833 MS.gene073984:CDS 30.0%
AGATTGAGGAAGTAAAAAGA+AGG - chr2.1:30192249-30192268 None:intergenic 30.0%
ATTTGGAGGAATCAAACAAT+AGG - chr2.1:30192133-30192152 None:intergenic 30.0%
GGAGATGAAGCAATTATAAA+AGG + chr2.1:30192180-30192199 MS.gene073984:CDS 30.0%
TGCGAGTATAATTACACTTA+TGG + chr2.1:30191898-30191917 MS.gene073984:CDS 30.0%
! AAGCAAAAAGTATATGGTGA+TGG - chr2.1:30192435-30192454 None:intergenic 30.0%
! AAGGTGAAGCAAAAAGTATA+TGG - chr2.1:30192441-30192460 None:intergenic 30.0%
! ATGAAAGTGGAAGATTTTGT+TGG - chr2.1:30191814-30191833 None:intergenic 30.0%
! CCACATCCAAAAATTGATTT+AGG - chr2.1:30191994-30192013 None:intergenic 30.0%
! GAACTTTGTTATATCTCCTA+AGG - chr2.1:30192460-30192479 None:intergenic 30.0%
! GGTACTGAATACATTAACTT+TGG + chr2.1:30191949-30191968 MS.gene073984:CDS 30.0%
! GTGGAAAACAATTTTCACAA+GGG - chr2.1:30191771-30191790 None:intergenic 30.0%
! TGGAAAACAATTTTCACAAG+GGG - chr2.1:30191770-30191789 None:intergenic 30.0%
! TGTGGAAAACAATTTTCACA+AGG - chr2.1:30191772-30191791 None:intergenic 30.0%
!! CCTAAATCAATTTTTGGATG+TGG + chr2.1:30191991-30192010 MS.gene073984:CDS 30.0%
!! GGTAACATAATCATTGATTC+AGG + chr2.1:30192318-30192337 MS.gene073984:CDS 30.0%
!! TATACTTTTTGCTTCACCTT+AGG + chr2.1:30192441-30192460 MS.gene073984:CDS 30.0%
AAGATGGAAAGATCATTGGT+TGG - chr2.1:30192585-30192604 None:intergenic 35.0%
ACCAATGATCTTTCCATCTT+TGG + chr2.1:30192585-30192604 MS.gene073984:CDS 35.0%
AGTTGTGAAACTAGTGATAG+TGG - chr2.1:30192084-30192103 None:intergenic 35.0%
TAAAAGAAGCCGTAAAGCTA+CGG + chr2.1:30192035-30192054 MS.gene073984:CDS 35.0%
TAGTTCTTGGAACAATCAGT+TGG - chr2.1:30192675-30192694 None:intergenic 35.0%
TCACAACTAGGTGATTCAAT+TGG + chr2.1:30192096-30192115 MS.gene073984:CDS 35.0%
TCCAAAGATGGAAAGATCAT+TGG - chr2.1:30192589-30192608 None:intergenic 35.0%
TTGTGCTCTATTTCCAAAGA+TGG - chr2.1:30192601-30192620 None:intergenic 35.0%
! CTATCACTAGTTTCACAACT+AGG + chr2.1:30192084-30192103 MS.gene073984:CDS 35.0%
! TTTTACTTCCTCAATCTCGA+AGG + chr2.1:30192252-30192271 MS.gene073984:CDS 35.0%
!! AACCAAGTTCTGAGGTTTAA+GGG - chr2.1:30192520-30192539 None:intergenic 35.0%
!! CAAAAAGTATATGGTGATGG+AGG - chr2.1:30192432-30192451 None:intergenic 35.0%
!! TCATAATAAGGAGTGTCTTG+TGG - chr2.1:30191790-30191809 None:intergenic 35.0%
AAAAGAAGCCGTAAAGCTAC+GGG + chr2.1:30192036-30192055 MS.gene073984:CDS 40.0%
AACACCAATAACTCCCTTCA+AGG - chr2.1:30192394-30192413 None:intergenic 40.0%
ACATGACAAACCCATGAAAG+TGG - chr2.1:30191827-30191846 None:intergenic 40.0%
CAACAACTCGTATTGCTTGT+TGG + chr2.1:30192551-30192570 MS.gene073984:CDS 40.0%
CAGAACTTGGTTGTTGATCA+TGG + chr2.1:30192528-30192547 MS.gene073984:CDS 40.0%
GGACAAAAAACTGTTCAGAC+AGG + chr2.1:30192285-30192304 MS.gene073984:CDS 40.0%
GGGAACATTGTACCCAAATA+AGG - chr2.1:30191750-30191769 None:intergenic 40.0%
TACCCTTAAACCTCAGAACT+TGG + chr2.1:30192515-30192534 MS.gene073984:CDS 40.0%
TCATTACTACCACATGGATG+TGG + chr2.1:30191862-30191881 MS.gene073984:CDS 40.0%
TGATCAACAACCAAGTTCTG+AGG - chr2.1:30192528-30192547 None:intergenic 40.0%
TTAGTAGGGTACGATCTCAA+AGG + chr2.1:30192630-30192649 MS.gene073984:CDS 40.0%
! ATGGAAAGATCATTGGTTGG+TGG - chr2.1:30192582-30192601 None:intergenic 40.0%
! GATTTGGTAGCTTCCTTGAA+GGG + chr2.1:30192378-30192397 MS.gene073984:CDS 40.0%
! TTTGAGTTTCCACATCCATG+TGG - chr2.1:30191874-30191893 None:intergenic 40.0%
!! CAACCAAGTTCTGAGGTTTA+AGG - chr2.1:30192521-30192540 None:intergenic 40.0%
!! TGATTTGGTAGCTTCCTTGA+AGG + chr2.1:30192377-30192396 MS.gene073984:CDS 40.0%
!!! CTGAACAGTTTTTTGTCCGA+CGG - chr2.1:30192283-30192302 None:intergenic 40.0%
CCATGTGGTAGTAATGAGCA+AGG - chr2.1:30191859-30191878 None:intergenic 45.0%
CCTTGCTCATTACTACCACA+TGG + chr2.1:30191856-30191875 MS.gene073984:CDS 45.0%
GCTTCCTTGAAGGGAGTTAT+TGG + chr2.1:30192387-30192406 MS.gene073984:CDS 45.0%
GGAACAATCAGTTGGAGCAA+AGG - chr2.1:30192667-30192686 None:intergenic 45.0%
TTCAGTACCCAAATCTCCAG+AGG - chr2.1:30191938-30191957 None:intergenic 45.0%
! CAACTAGACCCGTAGCTTTA+CGG - chr2.1:30192047-30192066 None:intergenic 45.0%
! TCTCACACCTCTGGAGATTT+GGG + chr2.1:30191928-30191947 MS.gene073984:CDS 45.0%
!! GAAGGGAGTTATTGGTGTTG+AGG + chr2.1:30192395-30192414 MS.gene073984:CDS 45.0%
ACACGGGGAGTGACCTTATT+TGG + chr2.1:30191734-30191753 MS.gene073984:CDS 50.0%
ACAGCAAGCCTTTCAACAGG+TGG - chr2.1:30191709-30191728 None:intergenic 50.0%
AGCAAGCCTTTCAACAGGTG+GGG - chr2.1:30191707-30191726 None:intergenic 50.0%
CAGCAAGCCTTTCAACAGGT+GGG - chr2.1:30191708-30191727 None:intergenic 50.0%
CGTAAAGCTACGGGTCTAGT+TGG + chr2.1:30192045-30192064 MS.gene073984:CDS 50.0%
GCAACAGCAAGCCTTTCAAC+AGG - chr2.1:30191712-30191731 None:intergenic 50.0%
GGTGATAACTCTCACACCTC+TGG + chr2.1:30191919-30191938 MS.gene073984:CDS 50.0%
GTTCAGACAGGTCAAACCGA+TGG + chr2.1:30192297-30192316 MS.gene073984:CDS 50.0%
! CACGGGGAGTGACCTTATTT+GGG + chr2.1:30191735-30191754 MS.gene073984:CDS 50.0%
! CTCTCACACCTCTGGAGATT+TGG + chr2.1:30191927-30191946 MS.gene073984:CDS 50.0%
!! AAGGCTTGCTGTTGCAGACA+CGG + chr2.1:30191717-30191736 MS.gene073984:CDS 50.0%
!! AATCTCGAAGGCATCACCGT+CGG + chr2.1:30192264-30192283 MS.gene073984:CDS 50.0%
CGGTGATGCCTTCGAGATTG+AGG - chr2.1:30192263-30192282 None:intergenic 55.0%
TAGGCACCCCACCTGTTGAA+AGG + chr2.1:30191698-30191717 MS.gene073984:CDS 55.0%
! GCTACGGGTCTAGTTGGTCT+TGG + chr2.1:30192051-30192070 MS.gene073984:CDS 55.0%
!! AGGCTTGCTGTTGCAGACAC+GGG + chr2.1:30191718-30191737 MS.gene073984:CDS 55.0%
!! GGTCTAGTTGGTCTTGGAGC+AGG + chr2.1:30192057-30192076 MS.gene073984:CDS 55.0%
!! GGCTTGCTGTTGCAGACACG+GGG + chr2.1:30191719-30191738 MS.gene073984:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 30191685 30192695 30191685 ID=MS.gene073984
chr2.1 mRNA 30191685 30192695 30191685 ID=MS.gene073984.t1;Parent=MS.gene073984
chr2.1 exon 30191685 30192695 30191685 ID=MS.gene073984.t1.exon1;Parent=MS.gene073984.t1
chr2.1 CDS 30191685 30192695 30191685 ID=cds.MS.gene073984.t1;Parent=MS.gene073984.t1
Gene Sequence

>MS.gene073984

ATGAAAATCTATATAGGCACCCCACCTGTTGAAAGGCTTGCTGTTGCAGACACGGGGAGTGACCTTATTTGGGTACAATGTTCCCCTTGTGAAAATTGTTTTCCACAAGACACTCCTTATTATGATCCAACAAAATCTTCCACTTTCATGGGTTTGTCATGTGACTCACAACCTTGCTCATTACTACCACATGGATGTGGAAACTCAAAGCAGTGCGAGTATAATTACACTTATGGTGATAACTCTCACACCTCTGGAGATTTGGGTACTGAATACATTAACTTTGGAGAAAAAGATGTTACATTTCCTAAATCAATTTTTGGATGTGGACATCAAAATGATGTCACATTTAAAAGAAGCCGTAAAGCTACGGGTCTAGTTGGTCTTGGAGCAGGACCACTATCACTAGTTTCACAACTAGGTGATTCAATTGGTCACAAATTTTCCTATTGTTTGATTCCTCCAAATTTAAACTCAAATAGCAAAATGAAATTTGGAGATGAAGCAATTATAAAAGGTAATGGCGTTGTGTCTACTTCCCTCATAATCAAATCTTCAAATCCTTCTTTTTACTTCCTCAATCTCGAAGGCATCACCGTCGGACAAAAAACTGTTCAGACAGGTCAAACCGATGGTAACATAATCATTGATTCAGGGACAACATTGACGTATCTTGAACAAAACTTTTACAATGATTTGGTAGCTTCCTTGAAGGGAGTTATTGGTGTTGAGGAAGTGAATGATCCTCCATCACCATATACTTTTTGCTTCACCTTAGGAGATATAACAAAGTTCCCTAATTTTGTGTTTCATTTTACGGGAGCTGATGTTACCCTTAAACCTCAGAACTTGGTTGTTGATCATGGCAACAACTCGTATTGCTTGTTGGTCGTACCACCAACCAATGATCTTTCCATCTTTGGAAATAGAGCACAATTTAATTTTTTAGTAGGGTACGATCTCAAAGGAAAAACAGTTTCCTTTGCTCCAACTGATTGTTCCAAGAACTAA
Protein sequence

>MS.gene073984.t1

MKIYIGTPPVERLAVADTGSDLIWVQCSPCENCFPQDTPYYDPTKSSTFMGLSCDSQPCSLLPHGCGNSKQCEYNYTYGDNSHTSGDLGTEYINFGEKDVTFPKSIFGCGHQNDVTFKRSRKATGLVGLGAGPLSLVSQLGDSIGHKFSYCLIPPNLNSNSKMKFGDEAIIKGNGVVSTSLIIKSSNPSFYFLNLEGITVGQKTVQTGQTDGNIIIDSGTTLTYLEQNFYNDLVASLKGVIGVEEVNDPPSPYTFCFTLGDITKFPNFVFHFTGADVTLKPQNLVVDHGNNSYCLLVVPPTNDLSIFGNRAQFNFLVGYDLKGKTVSFAPTDCSKN