Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074168.t1 | XP_003618792.1 | 97.5 | 157 | 4 | 0 | 1 | 157 | 1 | 157 | 5.60E-79 | 303.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074168.t1 | Q0WPX7 | 48.1 | 108 | 55 | 1 | 38 | 144 | 15 | 122 | 6.6e-20 | 98.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074168.t1 | G7KN78 | 97.5 | 157 | 4 | 0 | 1 | 157 | 1 | 157 | 4.0e-79 | 303.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051234 | MS.gene074168 | 0.811455 | 6.94E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074168.t1 | MTR_6g022020 | 97.452 | 157 | 4 | 0 | 1 | 157 | 1 | 157 | 8.12e-111 | 311 |
| MS.gene074168.t1 | MTR_6g022040 | 87.975 | 158 | 14 | 3 | 1 | 157 | 1 | 154 | 2.06e-92 | 265 |
| MS.gene074168.t1 | MTR_0086s0010 | 41.379 | 145 | 77 | 4 | 19 | 155 | 11 | 155 | 1.74e-27 | 105 |
| MS.gene074168.t1 | MTR_7g069610 | 46.296 | 108 | 55 | 1 | 32 | 136 | 13 | 120 | 1.99e-26 | 99.8 |
| MS.gene074168.t1 | MTR_1g101440 | 43.519 | 108 | 57 | 2 | 37 | 140 | 21 | 128 | 1.18e-24 | 97.4 |
| MS.gene074168.t1 | MTR_2g018860 | 37.500 | 128 | 72 | 2 | 33 | 152 | 25 | 152 | 1.50e-23 | 92.8 |
| MS.gene074168.t1 | MTR_4g102740 | 43.810 | 105 | 57 | 1 | 38 | 140 | 32 | 136 | 1.30e-22 | 90.9 |
| MS.gene074168.t1 | MTR_4g017810 | 51.220 | 82 | 40 | 0 | 58 | 139 | 26 | 107 | 3.04e-18 | 79.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074168.t1 | AT5G14360 | 54.386 | 171 | 56 | 5 | 1 | 157 | 1 | 163 | 2.17e-54 | 169 |
| MS.gene074168.t1 | AT5G40630 | 46.914 | 162 | 65 | 4 | 1 | 142 | 1 | 161 | 9.24e-43 | 139 |
| MS.gene074168.t1 | AT5G62100 | 49.057 | 106 | 53 | 1 | 38 | 142 | 15 | 120 | 1.22e-26 | 98.2 |
| MS.gene074168.t1 | AT5G62100 | 49.057 | 106 | 53 | 1 | 38 | 142 | 15 | 120 | 1.22e-26 | 98.2 |
| MS.gene074168.t1 | AT5G62100 | 48.148 | 108 | 55 | 1 | 38 | 144 | 15 | 122 | 7.43e-26 | 97.8 |
| MS.gene074168.t1 | AT5G62100 | 48.148 | 108 | 55 | 1 | 38 | 144 | 15 | 122 | 1.03e-25 | 97.8 |
| MS.gene074168.t1 | AT5G62100 | 48.148 | 108 | 55 | 1 | 38 | 144 | 15 | 122 | 6.49e-25 | 97.4 |
| MS.gene074168.t1 | AT5G62100 | 48.148 | 108 | 55 | 1 | 38 | 144 | 15 | 122 | 1.16e-24 | 96.7 |
| MS.gene074168.t1 | AT5G07220 | 42.017 | 119 | 68 | 1 | 38 | 155 | 21 | 139 | 1.23e-24 | 96.7 |
| MS.gene074168.t1 | AT5G52060 | 41.667 | 108 | 58 | 1 | 38 | 140 | 39 | 146 | 1.18e-21 | 89.4 |
| MS.gene074168.t1 | AT3G51780 | 41.071 | 112 | 50 | 2 | 36 | 131 | 8 | 119 | 1.95e-19 | 82.4 |
Find 30 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGAAGCAACTTCAACTTT+TGG | 0.327143 | 6.1:-5598438 | MS.gene074168:intron |
| AGCATATCTAAACTTGGCTT+TGG | 0.330269 | 6.1:-5598630 | MS.gene074168:CDS |
| TTCTTCACATGATTGGTGTT+AGG | 0.341495 | 6.1:-5597835 | MS.gene074168:CDS |
| TTTGTCACTTGTAACAAGTT+TGG | 0.343072 | 6.1:-5597912 | MS.gene074168:CDS |
| AATTTCAGGAGAATTGAAAA+TGG | 0.360866 | 6.1:-5597936 | MS.gene074168:intron |
| TAGAGAAGCCTCTGCTCTCT+TGG | 0.368216 | 6.1:+5597887 | None:intergenic |
| AGAAATGAAGCTACTTGGTT+TGG | 0.373066 | 6.1:-5597771 | MS.gene074168:CDS |
| TCACTACAATCCTTCTCAAT+TGG | 0.382631 | 6.1:+5598588 | None:intergenic |
| AGCTTCATTTCTTTGATAGC+TGG | 0.393914 | 6.1:+5597782 | None:intergenic |
| TCTTCACATGATTGGTGTTA+GGG | 0.396279 | 6.1:-5597834 | MS.gene074168:CDS |
| AATGAATTTCTTCACATGAT+TGG | 0.414769 | 6.1:-5597842 | MS.gene074168:CDS |
| ATCAAAGAAATGAAGCTACT+TGG | 0.456169 | 6.1:-5597776 | MS.gene074168:CDS |
| AGAGAGAGTAACAAAAGTGT+TGG | 0.471291 | 6.1:-5598513 | MS.gene074168:CDS |
| TGTAGAAGCATATCTAAACT+TGG | 0.485899 | 6.1:-5598636 | MS.gene074168:CDS |
| TGAAAATAGTGAAATCGAAT+GGG | 0.493131 | 6.1:-5598568 | MS.gene074168:CDS |
| GTGAAAATAGTGAAATCGAA+TGG | 0.497284 | 6.1:-5598569 | MS.gene074168:CDS |
| AAGCTACTTGGTTTGGCAAG+AGG | 0.530918 | 6.1:-5597764 | MS.gene074168:CDS |
| TATACTCTGATTGTGTAACT+AGG | 0.541450 | 6.1:+5597725 | None:intergenic |
| ATCGAATGGGAACTTAGGCC+TGG | 0.546005 | 6.1:-5598555 | MS.gene074168:CDS |
| AAAATAAGATGATGAAGTTG+AGG | 0.552877 | 6.1:-5598671 | None:intergenic |
| TGATGAAGTTGAGGTCAAAG+AGG | 0.559715 | 6.1:-5598662 | MS.gene074168:CDS |
| GAGCAGAGGCTTCTCTACAA+AGG | 0.569914 | 6.1:-5597881 | MS.gene074168:CDS |
| TTCTCTACAAAGGTAAAGAG+AGG | 0.582260 | 6.1:-5597871 | MS.gene074168:CDS |
| GAGTTTCAACTATGTCAAAG+TGG | 0.599297 | 6.1:-5598473 | MS.gene074168:CDS |
| AGTAGCATCTCCAATTGAGA+AGG | 0.608005 | 6.1:-5598598 | MS.gene074168:CDS |
| GTGAAATCGAATGGGAACTT+AGG | 0.612081 | 6.1:-5598560 | MS.gene074168:CDS |
| TGGTGTTAGGGACAAAGACA+AGG | 0.622779 | 6.1:-5597822 | MS.gene074168:CDS |
| GTTTGGAGCCAAGAGAGCAG+AGG | 0.625131 | 6.1:-5597895 | MS.gene074168:CDS |
| TCTCTACAAAGGTAAAGAGA+GGG | 0.650942 | 6.1:-5597870 | MS.gene074168:CDS |
| GAATGGGAACTTAGGCCTGG+TGG | 0.713354 | 6.1:-5598552 | MS.gene074168:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAATTGAACAATTATAATT+AGG | - | chr6.1:5598039-5598058 | MS.gene074168:intron | 10.0% |
| !! | TTTGAATTATTAACTAATAT+TGG | - | chr6.1:5598226-5598245 | MS.gene074168:intron | 10.0% |
| !! | GAATAATATAAATAAAAGCT+GGG | + | chr6.1:5598286-5598305 | None:intergenic | 15.0% |
| !! | TGAATAATATAAATAAAAGC+TGG | + | chr6.1:5598287-5598306 | None:intergenic | 15.0% |
| !! | TTAGTTAATAATTCAAAGTT+CGG | + | chr6.1:5598223-5598242 | None:intergenic | 15.0% |
| !!! | CAAAATTTTGAAATAATTTC+AGG | - | chr6.1:5598437-5598456 | MS.gene074168:intron | 15.0% |
| !! | CAACTATAAAAATAACTTTG+AGG | - | chr6.1:5598371-5598390 | MS.gene074168:intron | 20.0% |
| !! | CTTCAAAAAAAAAAAATGTG+AGG | - | chr6.1:5598403-5598422 | MS.gene074168:intron | 20.0% |
| ! | AATGAATTTCTTCACATGAT+TGG | - | chr6.1:5598545-5598564 | MS.gene074168:CDS | 25.0% |
| ! | AATTTCAGGAGAATTGAAAA+TGG | - | chr6.1:5598451-5598470 | MS.gene074168:CDS | 25.0% |
| ! | TGAAAATAGTGAAATCGAAT+GGG | - | chr6.1:5597819-5597838 | MS.gene074168:CDS | 25.0% |
| ! | TTGTGCATTACCATAAAATA+TGG | - | chr6.1:5598088-5598107 | MS.gene074168:intron | 25.0% |
| !! | ACAAAAGAATTAGTTTGAAC+CGG | + | chr6.1:5598015-5598034 | None:intergenic | 25.0% |
| !! | CTGTAGATAATAACTTTTGT+TGG | - | chr6.1:5598253-5598272 | MS.gene074168:intron | 25.0% |
| ATCAAAGAAATGAAGCTACT+TGG | - | chr6.1:5598611-5598630 | MS.gene074168:CDS | 30.0% | |
| CAAAAGGCAATACAAATGAA+AGG | + | chr6.1:5597989-5598008 | None:intergenic | 30.0% | |
| GTGAAAATAGTGAAATCGAA+TGG | - | chr6.1:5597818-5597837 | MS.gene074168:CDS | 30.0% | |
| TATACTCTGATTGTGTAACT+AGG | + | chr6.1:5598665-5598684 | None:intergenic | 30.0% | |
| ! | ATTGAAGCAACTTCAACTTT+TGG | - | chr6.1:5597949-5597968 | MS.gene074168:intron | 30.0% |
| ! | CACACTTTGACCATATTTTA+TGG | + | chr6.1:5598101-5598120 | None:intergenic | 30.0% |
| ! | TGTAGAAGCATATCTAAACT+TGG | - | chr6.1:5597751-5597770 | MS.gene074168:CDS | 30.0% |
| ! | TTTGTCACTTGTAACAAGTT+TGG | - | chr6.1:5598475-5598494 | MS.gene074168:CDS | 30.0% |
| !! | TTTGTATTGCCTTTTGTTTC+CGG | - | chr6.1:5597993-5598012 | MS.gene074168:intron | 30.0% |
| AGAAATGAAGCTACTTGGTT+TGG | - | chr6.1:5598616-5598635 | MS.gene074168:CDS | 35.0% | |
| AGCATATCTAAACTTGGCTT+TGG | - | chr6.1:5597757-5597776 | MS.gene074168:CDS | 35.0% | |
| AGGCAATACAAATGAAAGGT+TGG | + | chr6.1:5597985-5598004 | None:intergenic | 35.0% | |
| GAGTTTCAACTATGTCAAAG+TGG | - | chr6.1:5597914-5597933 | MS.gene074168:CDS | 35.0% | |
| TAATTCAAAGTTCGGCTAAG+AGG | + | chr6.1:5598215-5598234 | None:intergenic | 35.0% | |
| TAGTTTGAACCGGAAACAAA+AGG | + | chr6.1:5598005-5598024 | None:intergenic | 35.0% | |
| TCACTACAATCCTTCTCAAT+TGG | + | chr6.1:5597802-5597821 | None:intergenic | 35.0% | |
| TCTCTACAAAGGTAAAGAGA+GGG | - | chr6.1:5598517-5598536 | MS.gene074168:CDS | 35.0% | |
| TTCTCTACAAAGGTAAAGAG+AGG | - | chr6.1:5598516-5598535 | MS.gene074168:CDS | 35.0% | |
| ! | AGAGAGAGTAACAAAAGTGT+TGG | - | chr6.1:5597874-5597893 | MS.gene074168:CDS | 35.0% |
| ! | AGCTTCATTTCTTTGATAGC+TGG | + | chr6.1:5598608-5598627 | None:intergenic | 35.0% |
| ! | TTCTTCACATGATTGGTGTT+AGG | - | chr6.1:5598552-5598571 | MS.gene074168:CDS | 35.0% |
| !! | TCTTCACATGATTGGTGTTA+GGG | - | chr6.1:5598553-5598572 | MS.gene074168:CDS | 35.0% |
| !!! | ACACACAAGCAAGACTTTTT+TGG | + | chr6.1:5598334-5598353 | None:intergenic | 35.0% |
| AGTAGCATCTCCAATTGAGA+AGG | - | chr6.1:5597789-5597808 | MS.gene074168:CDS | 40.0% | |
| GTGAAATCGAATGGGAACTT+AGG | - | chr6.1:5597827-5597846 | MS.gene074168:CDS | 40.0% | |
| TGATGAAGTTGAGGTCAAAG+AGG | - | chr6.1:5597725-5597744 | MS.gene074168:CDS | 40.0% | |
| ! | TGGTGTTAGGGACAAAGACA+AGG | - | chr6.1:5598565-5598584 | MS.gene074168:CDS | 45.0% |
| ! | TTTTGTACAAGCATGCCACC+AGG | + | chr6.1:5597853-5597872 | None:intergenic | 45.0% |
| !! | AAGCTACTTGGTTTGGCAAG+AGG | - | chr6.1:5598623-5598642 | MS.gene074168:CDS | 45.0% |
| ATCGAATGGGAACTTAGGCC+TGG | - | chr6.1:5597832-5597851 | MS.gene074168:CDS | 50.0% | |
| GAGCAGAGGCTTCTCTACAA+AGG | - | chr6.1:5598506-5598525 | MS.gene074168:CDS | 50.0% | |
| TAGAGAAGCCTCTGCTCTCT+TGG | + | chr6.1:5598503-5598522 | None:intergenic | 50.0% | |
| GAATGGGAACTTAGGCCTGG+TGG | - | chr6.1:5597835-5597854 | MS.gene074168:CDS | 55.0% | |
| ! | GTTTGGAGCCAAGAGAGCAG+AGG | - | chr6.1:5598492-5598511 | MS.gene074168:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 5597724 | 5598685 | 5597724 | ID=MS.gene074168 |
| chr6.1 | mRNA | 5597724 | 5598685 | 5597724 | ID=MS.gene074168.t1;Parent=MS.gene074168 |
| chr6.1 | exon | 5598439 | 5598685 | 5598439 | ID=MS.gene074168.t1.exon1;Parent=MS.gene074168.t1 |
| chr6.1 | CDS | 5598439 | 5598685 | 5598439 | ID=cds.MS.gene074168.t1;Parent=MS.gene074168.t1 |
| chr6.1 | exon | 5597724 | 5597950 | 5597724 | ID=MS.gene074168.t1.exon2;Parent=MS.gene074168.t1 |
| chr6.1 | CDS | 5597724 | 5597950 | 5597724 | ID=cds.MS.gene074168.t1;Parent=MS.gene074168.t1 |
| Gene Sequence |
| Protein sequence |
