Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074290.t1 | XP_013451291.1 | 87.5 | 136 | 9 | 1 | 2 | 129 | 499 | 634 | 8.50E-57 | 229.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074290.t1 | A0A072U7K4 | 87.5 | 136 | 9 | 1 | 2 | 129 | 499 | 634 | 6.1e-57 | 229.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049821 | MS.gene074290 | 0.805812 | 1.11E-49 | -1.69E-46 |
| MS.gene055469 | MS.gene074290 | 0.80287 | 4.57E-49 | -1.69E-46 |
| MS.gene059725 | MS.gene074290 | 0.807081 | 6.02E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074290.t1 | MTR_6g017265 | 87.500 | 136 | 9 | 1 | 2 | 129 | 499 | 634 | 8.65e-76 | 236 |
| MS.gene074290.t1 | MTR_0024s0330 | 72.794 | 136 | 29 | 1 | 2 | 129 | 599 | 734 | 3.78e-63 | 204 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074290.t1 | AT5G38690 | 43.307 | 127 | 64 | 4 | 2 | 120 | 431 | 557 | 5.49e-20 | 84.7 |
| MS.gene074290.t1 | AT5G38690 | 43.307 | 127 | 64 | 4 | 2 | 120 | 431 | 557 | 5.49e-20 | 84.7 |
| MS.gene074290.t1 | AT1G67270 | 38.793 | 116 | 63 | 3 | 12 | 120 | 376 | 490 | 4.99e-15 | 70.5 |
| MS.gene074290.t1 | AT1G67780 | 34.188 | 117 | 68 | 3 | 12 | 120 | 383 | 498 | 1.87e-13 | 66.2 |
| MS.gene074290.t1 | AT1G67780 | 34.188 | 117 | 68 | 3 | 12 | 120 | 383 | 498 | 1.87e-13 | 66.2 |
Find 20 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGAAAAGTGGTTTGTTTA+TGG | 0.233780 | 6.2:-13475453 | MS.gene074290:CDS |
| AACTAAACTGAAGTCATCTT+TGG | 0.351197 | 6.2:+13475375 | None:intergenic |
| ATAACAGTGGTAATGCATTC+TGG | 0.369692 | 6.2:-13475645 | MS.gene074290:CDS |
| ACATTTCCTCTAGGAGAGAT+TGG | 0.390271 | 6.2:-13475401 | MS.gene074290:CDS |
| GCAAAAGCTGCTATATCAAA+TGG | 0.394679 | 6.2:-13476545 | MS.gene074290:CDS |
| AAGTCTTAAGCAAAAGTTGC+AGG | 0.414891 | 6.2:-13476576 | MS.gene074290:CDS |
| GAGAATTGAGCCTGAGTACT+TGG | 0.429535 | 6.2:-13475668 | MS.gene074290:CDS |
| CTGTTGAAGTTGATGAAAAG+TGG | 0.475523 | 6.2:-13475464 | MS.gene074290:CDS |
| CAAAAGCTGCTATATCAAAT+GGG | 0.521186 | 6.2:-13476544 | MS.gene074290:CDS |
| ACTGAGTTGCTTGAGGCAAA+AGG | 0.521553 | 6.2:-13476452 | MS.gene074290:CDS |
| GCAAAAGTTGCAGGATGAGA+TGG | 0.522189 | 6.2:-13476567 | MS.gene074290:CDS |
| CTTCAACAGCATCTTCATCA+TGG | 0.523773 | 6.2:+13475478 | None:intergenic |
| CATCATATTCTGAAATTGAG+AGG | 0.525339 | 6.2:+13476516 | None:intergenic |
| TTCTCACTGCATCACAACAT+TGG | 0.547808 | 6.2:+13475686 | None:intergenic |
| TGTTTATGGAGATGAACAGA+AGG | 0.583193 | 6.2:-13475439 | MS.gene074290:CDS |
| TGGAGATGAACAGAAGGATG+AGG | 0.596681 | 6.2:-13475433 | MS.gene074290:CDS |
| AGCTCGTACTGAGTTGCTTG+AGG | 0.622099 | 6.2:-13476459 | MS.gene074290:CDS |
| CCACTGTTATCCAAGTACTC+AGG | 0.639484 | 6.2:+13475658 | None:intergenic |
| TGGTAACCAATCTCTCCTAG+AGG | 0.673606 | 6.2:+13475395 | None:intergenic |
| CCTGAGTACTTGGATAACAG+TGG | 0.691208 | 6.2:-13475658 | MS.gene074290:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAAAAATATTATTATCATTC+AGG | + | chr6.2:13475885-13475904 | None:intergenic | 15.0% |
| !!! | TTTTTTTTATGATAACACTT+TGG | - | chr6.2:13475797-13475816 | MS.gene074290:intron | 15.0% |
| !! | TCAACTGAATTTAAACAAAT+TGG | + | chr6.2:13476197-13476216 | None:intergenic | 20.0% |
| !! | TCATATTCTTCATAAGAAAT+TGG | + | chr6.2:13476230-13476249 | None:intergenic | 20.0% |
| !! | TCTATCTGATAGTAATATTT+AGG | - | chr6.2:13475691-13475710 | MS.gene074290:CDS | 20.0% |
| !! | TTTCTTCTTATAACATTGTA+GGG | - | chr6.2:13476332-13476351 | MS.gene074290:intron | 20.0% |
| !!! | TTTGTTTTTTGTTTCTGAAA+TGG | - | chr6.2:13475384-13475403 | MS.gene074290:CDS | 20.0% |
| !!! | TTTTCTTCTTATAACATTGT+AGG | - | chr6.2:13476331-13476350 | MS.gene074290:intron | 20.0% |
| ! | AGAAAGTAAATGAAATGTTG+TGG | + | chr6.2:13475925-13475944 | None:intergenic | 25.0% |
| ! | ATCTGATAGTAATATTTAGG+TGG | - | chr6.2:13475694-13475713 | MS.gene074290:intron | 25.0% |
| ! | ATGATAAAGAACTTGAATGT+TGG | - | chr6.2:13475774-13475793 | MS.gene074290:intron | 25.0% |
| ! | GAAAGTAAATGAAATGTTGT+GGG | + | chr6.2:13475924-13475943 | None:intergenic | 25.0% |
| ! | GAATGATACTATTGAGATTT+AGG | - | chr6.2:13476497-13476516 | MS.gene074290:CDS | 25.0% |
| ! | GATCCAAATAAAATCAAGTT+TGG | + | chr6.2:13476287-13476306 | None:intergenic | 25.0% |
| ! | TGACTTCAGTAGTTATAATT+GGG | - | chr6.2:13475847-13475866 | MS.gene074290:intron | 25.0% |
| ! | TGGAAATTAAAAAGCTGTAA+TGG | - | chr6.2:13476418-13476437 | MS.gene074290:intron | 25.0% |
| ! | TTGACTTCAGTAGTTATAAT+TGG | - | chr6.2:13475846-13475865 | MS.gene074290:intron | 25.0% |
| !! | CATCCAAACTTGATTTTATT+TGG | - | chr6.2:13476281-13476300 | MS.gene074290:intron | 25.0% |
| !!! | TTTTAACTTGTTTACAACCT+AGG | + | chr6.2:13475742-13475761 | None:intergenic | 25.0% |
| AAACATCAGAACATACGTTT+AGG | + | chr6.2:13475624-13475643 | None:intergenic | 30.0% | |
| CAAAAGCTGCTATATCAAAT+GGG | - | chr6.2:13475499-13475518 | MS.gene074290:intron | 30.0% | |
| GAATAAGCTTTCTTGTTACA+AGG | - | chr6.2:13476442-13476461 | MS.gene074290:CDS | 30.0% | |
| GTTGTGTACATGTTATATAC+AGG | - | chr6.2:13475440-13475459 | MS.gene074290:CDS | 30.0% | |
| TCACTAAAAACATTTCCTCT+AGG | - | chr6.2:13476633-13476652 | MS.gene074290:intron | 30.0% | |
| TTATGATAACACTTTGGCTA+AGG | - | chr6.2:13475803-13475822 | MS.gene074290:intron | 30.0% | |
| !! | CATCATATTCTGAAATTGAG+AGG | + | chr6.2:13475530-13475549 | None:intergenic | 30.0% |
| !! | GATGAAAAGTGGTTTGTTTA+TGG | - | chr6.2:13476590-13476609 | MS.gene074290:intron | 30.0% |
| !! | TGTAGTTTATAAGAGTACCT+AGG | - | chr6.2:13475722-13475741 | MS.gene074290:intron | 30.0% |
| AAGTCTTAAGCAAAAGTTGC+AGG | - | chr6.2:13475467-13475486 | MS.gene074290:CDS | 35.0% | |
| AGATGCTAATGAACCTTGAA+TGG | - | chr6.2:13475983-13476002 | MS.gene074290:intron | 35.0% | |
| ATGTGGCATATGTTAATGCT+AGG | - | chr6.2:13476018-13476037 | MS.gene074290:intron | 35.0% | |
| ATTTGGATCTGCTATTGCAA+TGG | - | chr6.2:13476298-13476317 | MS.gene074290:intron | 35.0% | |
| GCAAAAGCTGCTATATCAAA+TGG | - | chr6.2:13475498-13475517 | MS.gene074290:intron | 35.0% | |
| TAGCATTAACATATGCCACA+TGG | + | chr6.2:13476019-13476038 | None:intergenic | 35.0% | |
| TGTGGCATATGTTAATGCTA+GGG | - | chr6.2:13476019-13476038 | MS.gene074290:intron | 35.0% | |
| TGTTTATGGAGATGAACAGA+AGG | - | chr6.2:13476604-13476623 | MS.gene074290:intron | 35.0% | |
| ! | AACACTTTGGCTAAGGTATT+AGG | - | chr6.2:13475810-13475829 | MS.gene074290:intron | 35.0% |
| ! | ATAACAGTGGTAATGCATTC+TGG | - | chr6.2:13476398-13476417 | MS.gene074290:intron | 35.0% |
| ! | GTAGTGGAACTTTGGATTTA+TGG | - | chr6.2:13476071-13476090 | MS.gene074290:intron | 35.0% |
| ! | TTGCTATTGTAGTGGAACTT+TGG | - | chr6.2:13476063-13476082 | MS.gene074290:intron | 35.0% |
| !! | CTGTTGAAGTTGATGAAAAG+TGG | - | chr6.2:13476579-13476598 | MS.gene074290:CDS | 35.0% |
| ATGAACACCATGTGACAGTT+TGG | + | chr6.2:13475417-13475436 | None:intergenic | 40.0% | |
| CTTCAACAGCATCTTCATCA+TGG | + | chr6.2:13476568-13476587 | None:intergenic | 40.0% | |
| GTGGCATATGTTAATGCTAG+GGG | - | chr6.2:13476020-13476039 | MS.gene074290:intron | 40.0% | |
| TCAACCAGTCATATTGTTCC+TGG | - | chr6.2:13476107-13476126 | MS.gene074290:intron | 40.0% | |
| TGCTATTCCAAACTGTCACA+TGG | - | chr6.2:13475407-13475426 | MS.gene074290:CDS | 40.0% | |
| TGGCATATGTTAATGCTAGG+GGG | - | chr6.2:13476021-13476040 | MS.gene074290:intron | 40.0% | |
| TTCTCACTGCATCACAACAT+TGG | + | chr6.2:13476360-13476379 | None:intergenic | 40.0% | |
| ! | ACATTTCCTCTAGGAGAGAT+TGG | - | chr6.2:13476642-13476661 | MS.gene074290:intron | 40.0% |
| !! | TGTGACACTTGCTATTGTAG+TGG | - | chr6.2:13476055-13476074 | MS.gene074290:intron | 40.0% |
| ACTGAGTTGCTTGAGGCAAA+AGG | - | chr6.2:13475591-13475610 | MS.gene074290:intron | 45.0% | |
| CCACTGTTATCCAAGTACTC+AGG | + | chr6.2:13476388-13476407 | None:intergenic | 45.0% | |
| CCTGAGTACTTGGATAACAG+TGG | - | chr6.2:13476385-13476404 | MS.gene074290:intron | 45.0% | |
| GAGAATTGAGCCTGAGTACT+TGG | - | chr6.2:13476375-13476394 | MS.gene074290:intron | 45.0% | |
| GCAAAAGTTGCAGGATGAGA+TGG | - | chr6.2:13475476-13475495 | MS.gene074290:CDS | 45.0% | |
| GCAACCAGGAACAATATGAC+TGG | + | chr6.2:13476114-13476133 | None:intergenic | 45.0% | |
| TCAACTCAGTGCAGATAGAG+AGG | + | chr6.2:13475961-13475980 | None:intergenic | 45.0% | |
| TGGAGATGAACAGAAGGATG+AGG | - | chr6.2:13476610-13476629 | MS.gene074290:intron | 45.0% | |
| TGGTAACCAATCTCTCCTAG+AGG | + | chr6.2:13476651-13476670 | None:intergenic | 45.0% | |
| TGGTTTCAGCACTCCATTCA+AGG | + | chr6.2:13475999-13476018 | None:intergenic | 45.0% | |
| ! | CATTTGCGTGGTTGATGTCA+TGG | - | chr6.2:13476137-13476156 | MS.gene074290:intron | 45.0% |
| !! | AATGGAGTGCTGAAACCATG+TGG | - | chr6.2:13476001-13476020 | MS.gene074290:intron | 45.0% |
| AGCTCGTACTGAGTTGCTTG+AGG | - | chr6.2:13475584-13475603 | MS.gene074290:intron | 50.0% | |
| CCACGCAAATGACAGCAACC+AGG | + | chr6.2:13476128-13476147 | None:intergenic | 55.0% | |
| ! | CCTGGTTGCTGTCATTTGCG+TGG | - | chr6.2:13476125-13476144 | MS.gene074290:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.2 | gene | 13475377 | 13476688 | 13475377 | ID=MS.gene074290 |
| chr6.2 | mRNA | 13475377 | 13476688 | 13475377 | ID=MS.gene074290.t1;Parent=MS.gene074290 |
| chr6.2 | exon | 13476686 | 13476688 | 13476686 | ID=MS.gene074290.t1.exon1;Parent=MS.gene074290.t1 |
| chr6.2 | CDS | 13476686 | 13476688 | 13476686 | ID=cds.MS.gene074290.t1;Parent=MS.gene074290.t1 |
| chr6.2 | exon | 13476438 | 13476603 | 13476438 | ID=MS.gene074290.t1.exon2;Parent=MS.gene074290.t1 |
| chr6.2 | CDS | 13476438 | 13476603 | 13476438 | ID=cds.MS.gene074290.t1;Parent=MS.gene074290.t1 |
| chr6.2 | exon | 13475626 | 13475712 | 13475626 | ID=MS.gene074290.t1.exon3;Parent=MS.gene074290.t1 |
| chr6.2 | CDS | 13475626 | 13475712 | 13475626 | ID=cds.MS.gene074290.t1;Parent=MS.gene074290.t1 |
| chr6.2 | exon | 13475377 | 13475510 | 13475377 | ID=MS.gene074290.t1.exon4;Parent=MS.gene074290.t1 |
| chr6.2 | CDS | 13475377 | 13475510 | 13475377 | ID=cds.MS.gene074290.t1;Parent=MS.gene074290.t1 |
| Gene Sequence |
| Protein sequence |
