Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074487.t1 | AET01058.2 | 90.6 | 53 | 5 | 0 | 95 | 147 | 235 | 287 | 3.40E-17 | 98.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074487.t1 | G7KFE1 | 90.6 | 53 | 5 | 0 | 95 | 147 | 235 | 287 | 2.4e-17 | 98.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051033 | MS.gene074487 | 0.819613 | 1.06E-52 | -1.69E-46 |
| MS.gene06118 | MS.gene074487 | -0.83956 | 1.47E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074487.t1 | MTR_5g098980 | 85.034 | 147 | 21 | 1 | 1 | 147 | 142 | 287 | 1.68e-47 | 163 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074487.t1 | AT4G35240 | 50.980 | 153 | 43 | 7 | 1 | 147 | 195 | 321 | 8.74e-24 | 96.3 |
| MS.gene074487.t1 | AT4G35240 | 50.980 | 153 | 43 | 7 | 1 | 147 | 195 | 321 | 8.74e-24 | 96.3 |
| MS.gene074487.t1 | AT4G35240 | 50.980 | 153 | 43 | 7 | 1 | 147 | 195 | 321 | 9.38e-24 | 96.3 |
| MS.gene074487.t1 | AT4G35240 | 50.980 | 153 | 43 | 7 | 1 | 147 | 195 | 321 | 9.38e-24 | 96.3 |
| MS.gene074487.t1 | AT2G17110 | 39.456 | 147 | 45 | 5 | 1 | 147 | 121 | 223 | 2.53e-11 | 60.8 |
Find 49 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTCCTTCTTCTTCCTTCTA+TGG | 0.169214 | 5.4:-78249808 | MS.gene074487:CDS |
| GTGGTGGTTGTTGTTGATAA+GGG | 0.304714 | 5.4:+78249884 | None:intergenic |
| GGTGGTGGTTGTTGTTGATA+AGG | 0.305171 | 5.4:+78249883 | None:intergenic |
| TCGTTGTTATATGGTGGATA+TGG | 0.320877 | 5.4:+78249853 | None:intergenic |
| CCGTAATAGTCGTTGTTATA+TGG | 0.324491 | 5.4:+78249844 | None:intergenic |
| GGAAGAAGGGATTCCTGATT+TGG | 0.328533 | 5.4:-78249608 | MS.gene074487:CDS |
| TCATCCTCGTCTTCCAAATC+AGG | 0.371482 | 5.4:+78249595 | None:intergenic |
| TTATATGGTGGATATGGATA+TGG | 0.377833 | 5.4:+78249859 | None:intergenic |
| TTTGGAGGAGAAGGTGGAGC+TGG | 0.400421 | 5.4:+78249745 | None:intergenic |
| ATTCACCGACATAGACAGTT+TGG | 0.405335 | 5.4:+78249932 | None:intergenic |
| CGAGGATGAAGTTGTTAAAC+AGG | 0.418339 | 5.4:-78249581 | None:intergenic |
| AGGTGGTGAAGATCCATAGA+AGG | 0.419627 | 5.4:+78249795 | None:intergenic |
| CCATATAACAACGACTATTA+CGG | 0.425392 | 5.4:-78249844 | MS.gene074487:CDS |
| AAGGATGTGAGGGAGGAAGA+AGG | 0.445552 | 5.4:-78249622 | MS.gene074487:CDS |
| AGGATGTGAGGGAGGAAGAA+GGG | 0.450088 | 5.4:-78249621 | MS.gene074487:CDS |
| TAGACAGTTTGGTTAGTGAT+AGG | 0.458109 | 5.4:+78249943 | None:intergenic |
| GGCTTTGAAGAAGGAGCTAT+AGG | 0.460005 | 5.4:+78249772 | None:intergenic |
| GGATATGGATATGGTGGTGG+TGG | 0.481629 | 5.4:+78249868 | None:intergenic |
| CCCTCACATCCTTTGAATCC+CGG | 0.483045 | 5.4:+78249632 | None:intergenic |
| GGAGGAGAAGGTGGAGCTGG+AGG | 0.488892 | 5.4:+78249748 | None:intergenic |
| GGAGAAGGTGGAGCTGGAGG+TGG | 0.502429 | 5.4:+78249751 | None:intergenic |
| ACGATTGAAGAAGGTGGAGG+TGG | 0.509774 | 5.4:+78249979 | None:intergenic |
| GCTGGAGGTGGCTTTGAAGA+AGG | 0.527528 | 5.4:+78249763 | None:intergenic |
| CACCCACGGCTACACCTAGC+CGG | 0.529747 | 5.4:-78249651 | MS.gene074487:CDS |
| TATGGTGGATATGGATATGG+TGG | 0.530798 | 5.4:+78249862 | None:intergenic |
| TACACCTAGCCGGGATTCAA+AGG | 0.532387 | 5.4:-78249641 | MS.gene074487:CDS |
| ACCCACGGCTACACCTAGCC+GGG | 0.551409 | 5.4:-78249650 | MS.gene074487:CDS |
| TAGAAGGAAGAAGAAGGAGG+AGG | 0.556191 | 5.4:+78249811 | None:intergenic |
| ATCCCGGCTAGGTGTAGCCG+TGG | 0.566699 | 5.4:+78249648 | None:intergenic |
| GGTGGATATGGATATGGTGG+TGG | 0.570106 | 5.4:+78249865 | None:intergenic |
| GAAGAAGGAGCTATAGGAGG+TGG | 0.576757 | 5.4:+78249778 | None:intergenic |
| TAATAGTCGTTGTTATATGG+TGG | 0.579607 | 5.4:+78249847 | None:intergenic |
| TGTTCGTAAACGATTGAAGA+AGG | 0.583851 | 5.4:+78249970 | None:intergenic |
| ACATCCTTTGAATCCCGGCT+AGG | 0.586247 | 5.4:+78249637 | None:intergenic |
| GATCCATAGAAGGAAGAAGA+AGG | 0.593503 | 5.4:+78249805 | None:intergenic |
| CCGGGATTCAAAGGATGTGA+GGG | 0.599644 | 5.4:-78249632 | MS.gene074487:CDS |
| ATAAGGGTTGTAATAAGATG+AGG | 0.604928 | 5.4:+78249900 | None:intergenic |
| AGAAGGAAGAAGAAGGAGGA+GGG | 0.610707 | 5.4:+78249812 | None:intergenic |
| GCCGGGATTCAAAGGATGTG+AGG | 0.611615 | 5.4:-78249633 | MS.gene074487:CDS |
| TCCCGGCTAGGTGTAGCCGT+GGG | 0.615427 | 5.4:+78249649 | None:intergenic |
| TAAACGATTGAAGAAGGTGG+AGG | 0.625943 | 5.4:+78249976 | None:intergenic |
| ACTAACCAAACTGTCTATGT+CGG | 0.632543 | 5.4:-78249937 | MS.gene074487:CDS |
| GGTGTGTATTGCGTCATCTG+TGG | 0.637398 | 5.4:+78249670 | None:intergenic |
| GATTCCTGATTTGGAAGACG+AGG | 0.647664 | 5.4:-78249599 | MS.gene074487:CDS |
| CCATAGAAGGAAGAAGAAGG+AGG | 0.654244 | 5.4:+78249808 | None:intergenic |
| TTTGAAGAAGGAGCTATAGG+AGG | 0.654960 | 5.4:+78249775 | None:intergenic |
| TCGTAAACGATTGAAGAAGG+TGG | 0.657233 | 5.4:+78249973 | None:intergenic |
| GGATTCAAAGGATGTGAGGG+AGG | 0.667509 | 5.4:-78249629 | MS.gene074487:CDS |
| GATGACGCAATACACACCCA+CGG | 0.696512 | 5.4:-78249665 | MS.gene074487:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATAAGGGTTGTAATAAGATG+AGG | + | chr5.4:78249685-78249704 | None:intergenic | 30.0% | |
| CCATATAACAACGACTATTA+CGG | - | chr5.4:78249738-78249757 | MS.gene074487:CDS | 30.0% | |
| TAATAGTCGTTGTTATATGG+TGG | + | chr5.4:78249738-78249757 | None:intergenic | 30.0% | |
| ! | AAATCCCAAGTAGAAGTTTT+TGG | + | chr5.4:78249858-78249877 | None:intergenic | 30.0% |
| !! | TTATATGGTGGATATGGATA+TGG | + | chr5.4:78249726-78249745 | None:intergenic | 30.0% |
| ACTAACCAAACTGTCTATGT+CGG | - | chr5.4:78249645-78249664 | MS.gene074487:CDS | 35.0% | |
| CCGTAATAGTCGTTGTTATA+TGG | + | chr5.4:78249741-78249760 | None:intergenic | 35.0% | |
| TAGACAGTTTGGTTAGTGAT+AGG | + | chr5.4:78249642-78249661 | None:intergenic | 35.0% | |
| TCCTCCAAAAACTTCTACTT+GGG | - | chr5.4:78249851-78249870 | MS.gene074487:CDS | 35.0% | |
| TGTTCGTAAACGATTGAAGA+AGG | + | chr5.4:78249615-78249634 | None:intergenic | 35.0% | |
| !! | TCGTTGTTATATGGTGGATA+TGG | + | chr5.4:78249732-78249751 | None:intergenic | 35.0% |
| ATTCACCGACATAGACAGTT+TGG | + | chr5.4:78249653-78249672 | None:intergenic | 40.0% | |
| CTCCTCCAAAAACTTCTACT+TGG | - | chr5.4:78249850-78249869 | MS.gene074487:CDS | 40.0% | |
| GATCCATAGAAGGAAGAAGA+AGG | + | chr5.4:78249780-78249799 | None:intergenic | 40.0% | |
| TAAACGATTGAAGAAGGTGG+AGG | + | chr5.4:78249609-78249628 | None:intergenic | 40.0% | |
| TCGTAAACGATTGAAGAAGG+TGG | + | chr5.4:78249612-78249631 | None:intergenic | 40.0% | |
| TTTGAAGAAGGAGCTATAGG+AGG | + | chr5.4:78249810-78249829 | None:intergenic | 40.0% | |
| ! | GTGGTGGTTGTTGTTGATAA+GGG | + | chr5.4:78249701-78249720 | None:intergenic | 40.0% |
| !! | CGTCGTTTTCGAAGAAATTG+AGG | + | chr5.4:78249881-78249900 | None:intergenic | 40.0% |
| !! | GTAGAAGTTTTTGGAGGAGA+AGG | + | chr5.4:78249849-78249868 | None:intergenic | 40.0% |
| !! | TATGGTGGATATGGATATGG+TGG | + | chr5.4:78249723-78249742 | None:intergenic | 40.0% |
| !! | TCCCAAGTAGAAGTTTTTGG+AGG | + | chr5.4:78249855-78249874 | None:intergenic | 40.0% |
| AGAAGGAAGAAGAAGGAGGA+GGG | + | chr5.4:78249773-78249792 | None:intergenic | 45.0% | |
| AGGTGGTGAAGATCCATAGA+AGG | + | chr5.4:78249790-78249809 | None:intergenic | 45.0% | |
| CCATAGAAGGAAGAAGAAGG+AGG | + | chr5.4:78249777-78249796 | None:intergenic | 45.0% | |
| CCTCCTTCTTCTTCCTTCTA+TGG | - | chr5.4:78249774-78249793 | MS.gene074487:CDS | 45.0% | |
| GGAAGAAGGGATTCCTGATT+TGG | - | chr5.4:78249974-78249993 | MS.gene074487:CDS | 45.0% | |
| TAGAAGGAAGAAGAAGGAGG+AGG | + | chr5.4:78249774-78249793 | None:intergenic | 45.0% | |
| TCATCCTCGTCTTCCAAATC+AGG | + | chr5.4:78249990-78250009 | None:intergenic | 45.0% | |
| ! | GGTGGTGGTTGTTGTTGATA+AGG | + | chr5.4:78249702-78249721 | None:intergenic | 45.0% |
| !! | GAAGTTTTTGGAGGAGAAGG+TGG | + | chr5.4:78249846-78249865 | None:intergenic | 45.0% |
| !! | GATTCCTGATTTGGAAGACG+AGG | - | chr5.4:78249983-78250002 | MS.gene074487:CDS | 45.0% |
| !! | GGCTTTGAAGAAGGAGCTAT+AGG | + | chr5.4:78249813-78249832 | None:intergenic | 45.0% |
| AAGGATGTGAGGGAGGAAGA+AGG | - | chr5.4:78249960-78249979 | MS.gene074487:CDS | 50.0% | |
| ACATCCTTTGAATCCCGGCT+AGG | + | chr5.4:78249948-78249967 | None:intergenic | 50.0% | |
| ACGATTGAAGAAGGTGGAGG+TGG | + | chr5.4:78249606-78249625 | None:intergenic | 50.0% | |
| AGGATGTGAGGGAGGAAGAA+GGG | - | chr5.4:78249961-78249980 | MS.gene074487:CDS | 50.0% | |
| CCCTCACATCCTTTGAATCC+CGG | + | chr5.4:78249953-78249972 | None:intergenic | 50.0% | |
| CCGGGATTCAAAGGATGTGA+GGG | - | chr5.4:78249950-78249969 | MS.gene074487:CDS | 50.0% | |
| GAAGAAGGAGCTATAGGAGG+TGG | + | chr5.4:78249807-78249826 | None:intergenic | 50.0% | |
| GATGACGCAATACACACCCA+CGG | - | chr5.4:78249917-78249936 | MS.gene074487:CDS | 50.0% | |
| GGATTCAAAGGATGTGAGGG+AGG | - | chr5.4:78249953-78249972 | MS.gene074487:CDS | 50.0% | |
| GGTGGATATGGATATGGTGG+TGG | + | chr5.4:78249720-78249739 | None:intergenic | 50.0% | |
| GGTGTGTATTGCGTCATCTG+TGG | + | chr5.4:78249915-78249934 | None:intergenic | 50.0% | |
| TACACCTAGCCGGGATTCAA+AGG | - | chr5.4:78249941-78249960 | MS.gene074487:CDS | 50.0% | |
| !! | GGATATGGATATGGTGGTGG+TGG | + | chr5.4:78249717-78249736 | None:intergenic | 50.0% |
| GCCGGGATTCAAAGGATGTG+AGG | - | chr5.4:78249949-78249968 | MS.gene074487:CDS | 55.0% | |
| TTTGGAGGAGAAGGTGGAGC+TGG | + | chr5.4:78249840-78249859 | None:intergenic | 55.0% | |
| !! | GCTGGAGGTGGCTTTGAAGA+AGG | + | chr5.4:78249822-78249841 | None:intergenic | 55.0% |
| ACCCACGGCTACACCTAGCC+GGG | - | chr5.4:78249932-78249951 | MS.gene074487:CDS | 65.0% | |
| ATCCCGGCTAGGTGTAGCCG+TGG | + | chr5.4:78249937-78249956 | None:intergenic | 65.0% | |
| CACCCACGGCTACACCTAGC+CGG | - | chr5.4:78249931-78249950 | MS.gene074487:CDS | 65.0% | |
| GGAGAAGGTGGAGCTGGAGG+TGG | + | chr5.4:78249834-78249853 | None:intergenic | 65.0% | |
| GGAGGAGAAGGTGGAGCTGG+AGG | + | chr5.4:78249837-78249856 | None:intergenic | 65.0% | |
| TCCCGGCTAGGTGTAGCCGT+GGG | + | chr5.4:78249936-78249955 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 78249582 | 78250022 | 78249582 | ID=MS.gene074487 |
| chr5.4 | mRNA | 78249582 | 78250022 | 78249582 | ID=MS.gene074487.t1;Parent=MS.gene074487 |
| chr5.4 | exon | 78249582 | 78250022 | 78249582 | ID=MS.gene074487.t1.exon1;Parent=MS.gene074487.t1 |
| chr5.4 | CDS | 78249582 | 78250022 | 78249582 | ID=cds.MS.gene074487.t1;Parent=MS.gene074487.t1 |
| Gene Sequence |
| Protein sequence |
