Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06118.t1 | KEH21283.1 | 94.2 | 86 | 2 | 1 | 1 | 83 | 1 | 86 | 3.20E-17 | 97.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06118.t1 | A0A072TWM2 | 94.2 | 86 | 2 | 1 | 1 | 83 | 1 | 86 | 2.3e-17 | 97.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05029 | MS.gene06118 | 0.812422 | 4.27E-51 | -1.69E-46 |
| MS.gene050320 | MS.gene06118 | 0.827594 | 1.43E-54 | -1.69E-46 |
| MS.gene050752 | MS.gene06118 | 0.819439 | 1.16E-52 | -1.69E-46 |
| MS.gene050955 | MS.gene06118 | 0.828379 | 9.29E-55 | -1.69E-46 |
| MS.gene051033 | MS.gene06118 | -0.822498 | 2.29E-53 | -1.69E-46 |
| MS.gene051903 | MS.gene06118 | 0.838128 | 3.46E-57 | -1.69E-46 |
| MS.gene054403 | MS.gene06118 | 0.82074 | 5.85E-53 | -1.69E-46 |
| MS.gene057130 | MS.gene06118 | 0.840869 | 6.71E-58 | -1.69E-46 |
| MS.gene059898 | MS.gene06118 | 0.800403 | 1.47E-48 | -1.69E-46 |
| MS.gene06118 | MS.gene063158 | 0.81298 | 3.23E-51 | -1.69E-46 |
| MS.gene06118 | MS.gene064416 | 0.814359 | 1.60E-51 | -1.69E-46 |
| MS.gene06118 | MS.gene065602 | 0.817672 | 2.92E-52 | -1.69E-46 |
| MS.gene06118 | MS.gene066455 | 0.824447 | 8.03E-54 | -1.69E-46 |
| MS.gene06118 | MS.gene06667 | 0.802288 | 6.02E-49 | -1.69E-46 |
| MS.gene06118 | MS.gene066751 | -0.823383 | 1.43E-53 | -1.69E-46 |
| MS.gene06118 | MS.gene067571 | 0.833136 | 6.34E-56 | -1.69E-46 |
| MS.gene06118 | MS.gene06883 | 0.81856 | 1.84E-52 | -1.69E-46 |
| MS.gene06118 | MS.gene06892 | 0.855068 | 8.08E-62 | -1.69E-46 |
| MS.gene06118 | MS.gene069310 | 0.856019 | 4.27E-62 | -1.69E-46 |
| MS.gene06118 | MS.gene06984 | 0.809015 | 2.33E-50 | -1.69E-46 |
| MS.gene06118 | MS.gene06989 | 0.810893 | 9.19E-51 | -1.69E-46 |
| MS.gene06118 | MS.gene069962 | 0.887601 | 1.24E-72 | -1.69E-46 |
| MS.gene06118 | MS.gene071751 | 0.805278 | 1.44E-49 | -1.69E-46 |
| MS.gene06118 | MS.gene072555 | 0.828919 | 6.88E-55 | -1.69E-46 |
| MS.gene06118 | MS.gene074487 | -0.83956 | 1.47E-57 | -1.69E-46 |
| MS.gene06118 | MS.gene07462 | -0.82124 | 4.48E-53 | -1.69E-46 |
| MS.gene06118 | MS.gene09056 | 0.811343 | 7.34E-51 | -1.69E-46 |
| MS.gene06118 | MS.gene20201 | 0.92124 | 4.61E-88 | -1.69E-46 |
| MS.gene06118 | MS.gene22843 | -0.800098 | 1.69E-48 | -1.69E-46 |
| MS.gene06118 | MS.gene22968 | 0.804558 | 2.04E-49 | -1.69E-46 |
| MS.gene06118 | MS.gene23059 | 0.800849 | 1.19E-48 | -1.69E-46 |
| MS.gene06118 | MS.gene23567 | 0.806311 | 8.75E-50 | -1.69E-46 |
| MS.gene06118 | MS.gene23936 | -0.835962 | 1.24E-56 | -1.69E-46 |
| MS.gene06118 | MS.gene23954 | 0.837535 | 4.91E-57 | -1.69E-46 |
| MS.gene06118 | MS.gene29245 | 0.802664 | 5.04E-49 | -1.69E-46 |
| MS.gene06118 | MS.gene29736 | 0.818679 | 1.73E-52 | -1.69E-46 |
| MS.gene06118 | MS.gene30158 | -0.821288 | 4.37E-53 | -1.69E-46 |
| MS.gene06118 | MS.gene31405 | 0.855635 | 5.53E-62 | -1.69E-46 |
| MS.gene06118 | MS.gene33664 | -0.803118 | 4.06E-49 | -1.69E-46 |
| MS.gene06118 | MS.gene35214 | 0.800943 | 1.14E-48 | -1.69E-46 |
| MS.gene06118 | MS.gene36098 | -0.865316 | 6.45E-65 | -1.69E-46 |
| MS.gene06118 | MS.gene40219 | -0.83138 | 1.72E-55 | -1.69E-46 |
| MS.gene06118 | MS.gene42671 | 0.815855 | 7.47E-52 | -1.69E-46 |
| MS.gene06118 | MS.gene46252 | 0.823855 | 1.11E-53 | -1.69E-46 |
| MS.gene06118 | MS.gene46373 | 0.828085 | 1.09E-54 | -1.69E-46 |
| MS.gene06118 | MS.gene48990 | -0.83021 | 3.34E-55 | -1.69E-46 |
| MS.gene06118 | MS.gene54282 | 0.81501 | 1.15E-51 | -1.69E-46 |
| MS.gene06118 | MS.gene55837 | 0.992977 | 1.19E-196 | -1.69E-46 |
| MS.gene06118 | MS.gene56436 | 0.814375 | 1.59E-51 | -1.69E-46 |
| MS.gene06118 | MS.gene56451 | 0.816633 | 5.00E-52 | -1.69E-46 |
| MS.gene06118 | MS.gene56656 | 0.825442 | 4.68E-54 | -1.69E-46 |
| MS.gene06118 | MS.gene59505 | 0.817491 | 3.21E-52 | -1.69E-46 |
| MS.gene06118 | MS.gene60717 | 0.823927 | 1.06E-53 | -1.69E-46 |
| MS.gene06118 | MS.gene61543 | 0.842346 | 2.73E-58 | -1.69E-46 |
| MS.gene06118 | MS.gene62834 | 0.828419 | 9.08E-55 | -1.69E-46 |
| MS.gene06118 | MS.gene64395 | 0.83905 | 2.00E-57 | -1.69E-46 |
| MS.gene06118 | MS.gene64806 | 0.815265 | 1.01E-51 | -1.69E-46 |
| MS.gene06118 | MS.gene65076 | 0.825345 | 4.93E-54 | -1.69E-46 |
| MS.gene06118 | MS.gene65554 | 0.800826 | 1.20E-48 | -1.69E-46 |
| MS.gene06118 | MS.gene66461 | 0.81956 | 1.09E-52 | -1.69E-46 |
| MS.gene06118 | MS.gene67485 | 0.814355 | 1.61E-51 | -1.69E-46 |
| MS.gene06118 | MS.gene72285 | 0.838835 | 2.27E-57 | -1.69E-46 |
| MS.gene06118 | MS.gene74672 | 0.8087 | 2.72E-50 | -1.69E-46 |
| MS.gene06118 | MS.gene74948 | 0.81684 | 4.49E-52 | -1.69E-46 |
| MS.gene06118 | MS.gene75171 | 0.827855 | 1.24E-54 | -1.69E-46 |
| MS.gene06118 | MS.gene77282 | 0.804442 | 2.16E-49 | -1.69E-46 |
| MS.gene06118 | MS.gene83326 | 0.817823 | 2.70E-52 | -1.69E-46 |
| MS.gene06118 | MS.gene85607 | -0.826111 | 3.25E-54 | -1.69E-46 |
| MS.gene06118 | MS.gene86265 | 0.813574 | 2.39E-51 | -1.69E-46 |
| MS.gene06118 | MS.gene86392 | 0.849264 | 3.61E-60 | -1.69E-46 |
| MS.gene06118 | MS.gene89027 | 0.845801 | 3.24E-59 | -1.69E-46 |
| MS.gene06118 | MS.gene90833 | 0.813245 | 2.82E-51 | -1.69E-46 |
| MS.gene06118 | MS.gene92135 | 0.824166 | 9.35E-54 | -1.69E-46 |
| MS.gene06118 | MS.gene92557 | 0.806424 | 8.28E-50 | -1.69E-46 |
| MS.gene06118 | MS.gene93973 | 0.800588 | 1.34E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06118.t1 | MTR_8g099965 | 94.186 | 86 | 2 | 1 | 1 | 83 | 1 | 86 | 4.33e-51 | 155 |
| MS.gene06118.t1 | MTR_8g027380 | 61.905 | 42 | 16 | 0 | 3 | 44 | 8 | 49 | 5.93e-13 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06118.t1 | AT4G10810 | 62.500 | 48 | 18 | 0 | 4 | 51 | 16 | 63 | 2.95e-13 | 59.3 |
Find 32 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAACTTCTCTTTAGATATC+AGG | 0.264418 | 4.2:+3939676 | None:intergenic |
| TTCCGCTTTCTTCTCTTCCT+TGG | 0.296008 | 4.2:-3939619 | MS.gene06118:CDS |
| TCATCGTCTTCGTCGTTATC+AGG | 0.344564 | 4.2:+3939558 | None:intergenic |
| GGAGGTTGGTGATATCGTTT+GGG | 0.357316 | 4.2:+3939472 | None:intergenic |
| GGGAGGTTGGTGATATCGTT+TGG | 0.364836 | 4.2:+3939471 | None:intergenic |
| GATCAGATGGCAACCTGGGT+GGG | 0.426814 | 4.2:-3939654 | MS.gene06118:CDS |
| GGGGTCTTAACGGAGGGATC+AGG | 0.428723 | 4.2:+3939492 | None:intergenic |
| GGTGGTGGTGGTGACGGAGT+CGG | 0.448510 | 4.2:+3939530 | None:intergenic |
| ATCAGATGGCAACCTGGGTG+GGG | 0.454986 | 4.2:-3939653 | MS.gene06118:CDS |
| CCTGATATCTAAAGAGAAGT+TGG | 0.455522 | 4.2:-3939676 | MS.gene06118:CDS |
| GTTGACGGTGGTGGTGGTGG+TGG | 0.455838 | 4.2:+3939518 | None:intergenic |
| ATTGTTGACGGTGGTGGTGG+TGG | 0.457641 | 4.2:+3939515 | None:intergenic |
| ATCAGGATTGTTGACGGTGG+TGG | 0.467123 | 4.2:+3939509 | None:intergenic |
| GATATCGTTTGGGGTCTTAA+CGG | 0.478557 | 4.2:+3939482 | None:intergenic |
| AGGATTGTTGACGGTGGTGG+TGG | 0.495328 | 4.2:+3939512 | None:intergenic |
| TAAAGAGAAGTTGGATCAGA+TGG | 0.515604 | 4.2:-3939667 | MS.gene06118:CDS |
| AGAGAAGAAAGCGGAAGAGA+CGG | 0.533991 | 4.2:+3939626 | None:intergenic |
| AGTTGGATCAGATGGCAACC+TGG | 0.543370 | 4.2:-3939659 | MS.gene06118:CDS |
| GACGCAAGAAAAGCGTTCCA+AGG | 0.548791 | 4.2:+3939602 | None:intergenic |
| GGATCAGATGGCAACCTGGG+TGG | 0.570514 | 4.2:-3939655 | MS.gene06118:CDS |
| GAAGAAAGCGGAAGAGACGG+TGG | 0.578991 | 4.2:+3939629 | None:intergenic |
| GTTGGATCAGATGGCAACCT+GGG | 0.581479 | 4.2:-3939658 | MS.gene06118:CDS |
| GGAGGGATCAGGATTGTTGA+CGG | 0.583608 | 4.2:+3939503 | None:intergenic |
| TCGTTTGGGGTCTTAACGGA+GGG | 0.589276 | 4.2:+3939486 | None:intergenic |
| ATCGTTTGGGGTCTTAACGG+AGG | 0.613773 | 4.2:+3939485 | None:intergenic |
| AGAGACGGTGGACCCCACCC+AGG | 0.649497 | 4.2:+3939641 | None:intergenic |
| TCGTCTTCGTCGTTATCAGG+AGG | 0.685685 | 4.2:+3939561 | None:intergenic |
| TTCCAAGGAAGAGAAGAAAG+CGG | 0.690072 | 4.2:+3939617 | None:intergenic |
| GTTATCAGGAGGATCAGAAG+TGG | 0.695959 | 4.2:+3939572 | None:intergenic |
| GAGGTTGGTGATATCGTTTG+GGG | 0.715129 | 4.2:+3939473 | None:intergenic |
| GGGATCAGGATTGTTGACGG+TGG | 0.721497 | 4.2:+3939506 | None:intergenic |
| TCGACCACGATGGAGAACAA+CGG | 0.739992 | 4.2:-3939702 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CCAACTTCTCTTTAGATATC+AGG | + | chr4.2:3939484-3939503 | None:intergenic | 35.0% | |
| CCTGATATCTAAAGAGAAGT+TGG | - | chr4.2:3939481-3939500 | MS.gene06118:CDS | 35.0% | |
| TAAAGAGAAGTTGGATCAGA+TGG | - | chr4.2:3939490-3939509 | MS.gene06118:CDS | 35.0% | |
| TTCCAAGGAAGAGAAGAAAG+CGG | + | chr4.2:3939543-3939562 | None:intergenic | 40.0% | |
| ! | GATATCGTTTGGGGTCTTAA+CGG | + | chr4.2:3939678-3939697 | None:intergenic | 40.0% |
| AGAGAAGAAAGCGGAAGAGA+CGG | + | chr4.2:3939534-3939553 | None:intergenic | 45.0% | |
| GTTATCAGGAGGATCAGAAG+TGG | + | chr4.2:3939588-3939607 | None:intergenic | 45.0% | |
| TCATCGTCTTCGTCGTTATC+AGG | + | chr4.2:3939602-3939621 | None:intergenic | 45.0% | |
| TTCCGCTTTCTTCTCTTCCT+TGG | - | chr4.2:3939538-3939557 | MS.gene06118:CDS | 45.0% | |
| !! | GAGGTTGGTGATATCGTTTG+GGG | + | chr4.2:3939687-3939706 | None:intergenic | 45.0% |
| !! | GGAGGTTGGTGATATCGTTT+GGG | + | chr4.2:3939688-3939707 | None:intergenic | 45.0% |
| AGTTGGATCAGATGGCAACC+TGG | - | chr4.2:3939498-3939517 | MS.gene06118:CDS | 50.0% | |
| ATCAGGATTGTTGACGGTGG+TGG | + | chr4.2:3939651-3939670 | None:intergenic | 50.0% | |
| GGAGGGATCAGGATTGTTGA+CGG | + | chr4.2:3939657-3939676 | None:intergenic | 50.0% | |
| GTTGGATCAGATGGCAACCT+GGG | - | chr4.2:3939499-3939518 | MS.gene06118:CDS | 50.0% | |
| TCGTCTTCGTCGTTATCAGG+AGG | + | chr4.2:3939599-3939618 | None:intergenic | 50.0% | |
| ! | ATCGTTTGGGGTCTTAACGG+AGG | + | chr4.2:3939675-3939694 | None:intergenic | 50.0% |
| ! | TCGTTTGGGGTCTTAACGGA+GGG | + | chr4.2:3939674-3939693 | None:intergenic | 50.0% |
| !! | GACGCAAGAAAAGCGTTCCA+AGG | + | chr4.2:3939558-3939577 | None:intergenic | 50.0% |
| !! | GGGAGGTTGGTGATATCGTT+TGG | + | chr4.2:3939689-3939708 | None:intergenic | 50.0% |
| ATCAGATGGCAACCTGGGTG+GGG | - | chr4.2:3939504-3939523 | MS.gene06118:CDS | 55.0% | |
| GAAGAAAGCGGAAGAGACGG+TGG | + | chr4.2:3939531-3939550 | None:intergenic | 55.0% | |
| GATCAGATGGCAACCTGGGT+GGG | - | chr4.2:3939503-3939522 | MS.gene06118:CDS | 55.0% | |
| GGGATCAGGATTGTTGACGG+TGG | + | chr4.2:3939654-3939673 | None:intergenic | 55.0% | |
| ! | AGGATTGTTGACGGTGGTGG+TGG | + | chr4.2:3939648-3939667 | None:intergenic | 55.0% |
| !! | ATTGTTGACGGTGGTGGTGG+TGG | + | chr4.2:3939645-3939664 | None:intergenic | 55.0% |
| GGATCAGATGGCAACCTGGG+TGG | - | chr4.2:3939502-3939521 | MS.gene06118:CDS | 60.0% | |
| GGGGTCTTAACGGAGGGATC+AGG | + | chr4.2:3939668-3939687 | None:intergenic | 60.0% | |
| !! | GGTGGTGGTGGTGACGGAGT+CGG | + | chr4.2:3939630-3939649 | None:intergenic | 65.0% |
| !! | GGTGGTGGTGGTGGTGGTGA+CGG | + | chr4.2:3939636-3939655 | None:intergenic | 65.0% |
| !! | GTTGACGGTGGTGGTGGTGG+TGG | + | chr4.2:3939642-3939661 | None:intergenic | 65.0% |
| AGAGACGGTGGACCCCACCC+AGG | + | chr4.2:3939519-3939538 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 3939464 | 3939715 | 3939464 | ID=MS.gene06118 |
| chr4.2 | mRNA | 3939464 | 3939715 | 3939464 | ID=MS.gene06118.t1;Parent=MS.gene06118 |
| chr4.2 | exon | 3939464 | 3939715 | 3939464 | ID=MS.gene06118.t1.exon1;Parent=MS.gene06118.t1 |
| chr4.2 | CDS | 3939464 | 3939715 | 3939464 | ID=cds.MS.gene06118.t1;Parent=MS.gene06118.t1 |
| Gene Sequence |
| Protein sequence |
