Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074516.t1 | XP_013451322.1 | 85.9 | 78 | 11 | 0 | 1 | 78 | 39 | 116 | 1.20E-21 | 112.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074516.t1 | A0A072U6V3 | 85.9 | 78 | 11 | 0 | 1 | 78 | 39 | 116 | 8.6e-22 | 112.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060572 | MS.gene074516 | 0.818499 | 1.90E-52 | -1.69E-46 |
| MS.gene061040 | MS.gene074516 | -0.800215 | 1.60E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074516.t1 | MTR_6g018800 | 93.590 | 78 | 5 | 0 | 1 | 78 | 39 | 116 | 3.14e-41 | 131 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 7 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAAGCCAAAATTGCATT+GGG | 0.411282 | 6.1:-4822539 | MS.gene074516:CDS |
| CAAAGAAGCCAAAATTGCAT+TGG | 0.485599 | 6.1:-4822540 | MS.gene074516:CDS |
| AGCTTTCACAATTGTTCAAG+AGG | 0.501859 | 6.1:+4822605 | None:intergenic |
| TATAGCGAGAACAGTGCTCA+AGG | 0.594448 | 6.1:-4822655 | MS.gene074516:CDS |
| AGAAGCCAAAATTGCATTGG+GGG | 0.610391 | 6.1:-4822537 | None:intergenic |
| AAGAAGCCAAAATTGCATTG+GGG | 0.668833 | 6.1:-4822538 | MS.gene074516:CDS |
| AAGTTTAAGACAAAACCCAA+AGG | 0.697268 | 6.1:-4822821 | MS.gene074516:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCTTATTTGTTTTTAAATTT+TGG | - | chr6.1:4822620-4822639 | MS.gene074516:CDS | 10.0% |
| !! | AAAAAACAAAAAGAACCTTT+GGG | + | chr6.1:4822595-4822614 | None:intergenic | 20.0% |
| ! | ACACACAAAATACATATCAA+CGG | + | chr6.1:4822669-4822688 | None:intergenic | 25.0% |
| ! | CAAAAAACAAAAAGAACCTT+TGG | + | chr6.1:4822596-4822615 | None:intergenic | 25.0% |
| !!! | TATTTTGTGTGTTCTGAATT+GGG | - | chr6.1:4822677-4822696 | MS.gene074516:CDS | 25.0% |
| AAAGAAGCCAAAATTGCATT+GGG | - | chr6.1:4822859-4822878 | MS.gene074516:CDS | 30.0% | |
| TCGAAAAATGAAGACTCAAA+GGG | - | chr6.1:4822821-4822840 | MS.gene074516:intron | 30.0% | |
| TTCGAAAAATGAAGACTCAA+AGG | - | chr6.1:4822820-4822839 | MS.gene074516:intron | 30.0% | |
| ! | AAGTTTAAGACAAAACCCAA+AGG | - | chr6.1:4822577-4822596 | MS.gene074516:CDS | 30.0% |
| !! | GTATTTTGTGTGTTCTGAAT+TGG | - | chr6.1:4822676-4822695 | MS.gene074516:CDS | 30.0% |
| AAAAATGAAGACTCAAAGGG+TGG | - | chr6.1:4822824-4822843 | MS.gene074516:CDS | 35.0% | |
| AAGAAGCCAAAATTGCATTG+GGG | - | chr6.1:4822860-4822879 | MS.gene074516:CDS | 35.0% | |
| AGCTTTCACAATTGTTCAAG+AGG | + | chr6.1:4822796-4822815 | None:intergenic | 35.0% | |
| CAAAGAAGCCAAAATTGCAT+TGG | - | chr6.1:4822858-4822877 | MS.gene074516:CDS | 35.0% | |
| AGAAGCCAAAATTGCATTGG+GGG | - | chr6.1:4822861-4822880 | MS.gene074516:CDS | 40.0% | |
| TGCAGATGATGCCAAAAAGA+AGG | - | chr6.1:4822703-4822722 | MS.gene074516:intron | 40.0% | |
| TATAGCGAGAACAGTGCTCA+AGG | - | chr6.1:4822743-4822762 | MS.gene074516:intron | 45.0% | |
| ! | ATTGCCTCGTGCCTTCTTTT+TGG | + | chr6.1:4822717-4822736 | None:intergenic | 45.0% |
| ! | GATGCCAAAAAGAAGGCACG+AGG | - | chr6.1:4822710-4822729 | MS.gene074516:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 4822538 | 4822882 | 4822538 | ID=MS.gene074516 |
| chr6.1 | mRNA | 4822538 | 4822882 | 4822538 | ID=MS.gene074516.t1;Parent=MS.gene074516 |
| chr6.1 | exon | 4822822 | 4822882 | 4822822 | ID=MS.gene074516.t1.exon1;Parent=MS.gene074516.t1 |
| chr6.1 | CDS | 4822822 | 4822882 | 4822822 | ID=cds.MS.gene074516.t1;Parent=MS.gene074516.t1 |
| chr6.1 | exon | 4822538 | 4822712 | 4822538 | ID=MS.gene074516.t1.exon2;Parent=MS.gene074516.t1 |
| chr6.1 | CDS | 4822538 | 4822712 | 4822538 | ID=cds.MS.gene074516.t1;Parent=MS.gene074516.t1 |
| Gene Sequence |
| Protein sequence |
