Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07463.t1 | XP_013468822.1 | 100 | 77 | 0 | 0 | 1 | 77 | 57 | 133 | 2.00E-29 | 137.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07463.t1 | A0A072VLL2 | 100.0 | 77 | 0 | 0 | 1 | 77 | 57 | 133 | 1.4e-29 | 137.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056903 | MS.gene07463 | -0.814429 | 1.55E-51 | -1.69E-46 |
| MS.gene057246 | MS.gene07463 | -0.814178 | 1.76E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07463.t1 | MTR_1g077210 | 100.000 | 77 | 0 | 0 | 1 | 77 | 57 | 133 | 5.41e-48 | 152 |
| MS.gene07463.t1 | MTR_1g009030 | 88.312 | 77 | 9 | 0 | 1 | 77 | 57 | 133 | 9.98e-43 | 137 |
| MS.gene07463.t1 | MTR_3g109280 | 88.312 | 77 | 9 | 0 | 1 | 77 | 57 | 133 | 1.26e-42 | 138 |
| MS.gene07463.t1 | MTR_0054s0080 | 88.312 | 77 | 9 | 0 | 1 | 77 | 57 | 133 | 2.55e-42 | 137 |
| MS.gene07463.t1 | MTR_6g066040 | 67.105 | 76 | 25 | 0 | 1 | 76 | 57 | 132 | 8.89e-32 | 110 |
| MS.gene07463.t1 | MTR_6g066090 | 64.407 | 59 | 21 | 0 | 18 | 76 | 1 | 59 | 1.28e-21 | 82.4 |
| MS.gene07463.t1 | MTR_5g068640 | 38.158 | 76 | 46 | 1 | 1 | 76 | 55 | 129 | 1.77e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07463.t1 | AT4G31330 | 84.416 | 77 | 12 | 0 | 1 | 77 | 57 | 133 | 1.23e-40 | 133 |
| MS.gene07463.t1 | AT5G10580 | 81.818 | 77 | 14 | 0 | 1 | 77 | 57 | 133 | 2.18e-40 | 131 |
| MS.gene07463.t1 | AT5G10580 | 81.818 | 77 | 14 | 0 | 1 | 77 | 57 | 133 | 9.85e-40 | 131 |
| MS.gene07463.t1 | AT5G10580 | 81.818 | 77 | 14 | 0 | 1 | 77 | 57 | 133 | 9.85e-40 | 131 |
| MS.gene07463.t1 | AT5G10580 | 81.818 | 77 | 14 | 0 | 1 | 77 | 57 | 133 | 1.18e-39 | 131 |
| MS.gene07463.t1 | AT5G24790 | 68.571 | 70 | 22 | 0 | 1 | 70 | 57 | 126 | 3.38e-31 | 109 |
Find 14 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTCTCAATGATTCCATTTA+TGG | 0.062471 | 1.3:+55488033 | MS.gene07463:CDS |
| TGGAGCTCATAGTGAATTTA+TGG | 0.212693 | 1.3:+55488053 | MS.gene07463:CDS |
| AACCCTACGTAATCTAATAA+TGG | 0.254699 | 1.3:+55487927 | MS.gene07463:CDS |
| TTCACTATGAGCTCCATAAA+TGG | 0.279592 | 1.3:-55488046 | None:intergenic |
| AATAATGGGATCAACACTTA+TGG | 0.371912 | 1.3:+55487942 | MS.gene07463:CDS |
| CATTCTACTCTCAGCTGGTT+TGG | 0.383869 | 1.3:+55487975 | MS.gene07463:CDS |
| ACCCTACGTAATCTAATAAT+GGG | 0.403558 | 1.3:+55487928 | MS.gene07463:CDS |
| ATGAGAATAAGAATATAGTT+AGG | 0.446792 | 1.3:-55488098 | None:intergenic |
| TCCCATTATTAGATTACGTA+GGG | 0.517944 | 1.3:-55487929 | None:intergenic |
| ACATCCATTCTACTCTCAGC+TGG | 0.523277 | 1.3:+55487970 | MS.gene07463:CDS |
| CAAACCAGCTGAGAGTAGAA+TGG | 0.570136 | 1.3:-55487974 | None:intergenic |
| CCATAAATGGAATCATTGAG+AGG | 0.651845 | 1.3:-55488033 | None:intergenic |
| TACGTAGGGTTTGAACAGCT+AGG | 0.661798 | 1.3:-55487915 | None:intergenic |
| ATCCCATTATTAGATTACGT+AGG | 0.669460 | 1.3:-55487930 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGAGAATAAGAATATAGTT+AGG | - | chr1.3:55488101-55488120 | None:intergenic | 20.0% |
| AACCCTACGTAATCTAATAA+TGG | + | chr1.3:55487927-55487946 | MS.gene07463:CDS | 30.0% | |
| ACCCTACGTAATCTAATAAT+GGG | + | chr1.3:55487928-55487947 | MS.gene07463:CDS | 30.0% | |
| ATCCCATTATTAGATTACGT+AGG | - | chr1.3:55487933-55487952 | None:intergenic | 30.0% | |
| TCCCATTATTAGATTACGTA+GGG | - | chr1.3:55487932-55487951 | None:intergenic | 30.0% | |
| ! | AATAATGGGATCAACACTTA+TGG | + | chr1.3:55487942-55487961 | MS.gene07463:CDS | 30.0% |
| !! | TAGGAGTGTGACATATTTTA+GGG | - | chr1.3:55488082-55488101 | None:intergenic | 30.0% |
| !! | TTAGGAGTGTGACATATTTT+AGG | - | chr1.3:55488083-55488102 | None:intergenic | 30.0% |
| CCATAAATGGAATCATTGAG+AGG | - | chr1.3:55488036-55488055 | None:intergenic | 35.0% | |
| CCTCTCAATGATTCCATTTA+TGG | + | chr1.3:55488033-55488052 | MS.gene07463:CDS | 35.0% | |
| TGGAGCTCATAGTGAATTTA+TGG | + | chr1.3:55488053-55488072 | MS.gene07463:CDS | 35.0% | |
| TTCACTATGAGCTCCATAAA+TGG | - | chr1.3:55488049-55488068 | None:intergenic | 35.0% | |
| ACATCCATTCTACTCTCAGC+TGG | + | chr1.3:55487970-55487989 | MS.gene07463:CDS | 45.0% | |
| CAAACCAGCTGAGAGTAGAA+TGG | - | chr1.3:55487977-55487996 | None:intergenic | 45.0% | |
| CATTCTACTCTCAGCTGGTT+TGG | + | chr1.3:55487975-55487994 | MS.gene07463:CDS | 45.0% | |
| TACGTAGGGTTTGAACAGCT+AGG | - | chr1.3:55487918-55487937 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 55487895 | 55488126 | 55487895 | ID=MS.gene07463 |
| chr1.3 | mRNA | 55487895 | 55488126 | 55487895 | ID=MS.gene07463.t1;Parent=MS.gene07463 |
| chr1.3 | exon | 55487895 | 55488126 | 55487895 | ID=MS.gene07463.t1.exon1;Parent=MS.gene07463.t1 |
| chr1.3 | CDS | 55487895 | 55488126 | 55487895 | ID=cds.MS.gene07463.t1;Parent=MS.gene07463.t1 |
| Gene Sequence |
| Protein sequence |
