AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07508


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07508.t1 MTR_1g077890 99.444 180 1 0 1 180 271 450 7.22e-133 379
MS.gene07508.t1 MTR_3g080850 83.333 168 28 0 6 173 274 441 5.37e-93 278
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07508.t1 AT1G64660 76.023 171 41 0 1 171 271 441 1.99e-85 258

Find 46 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATAGTTCAAATGCTATTT+TGG 0.073928 1.3:-55603702 None:intergenic
AAAGTGATTCAATTCTTATT+AGG 0.168129 1.3:-55604178 None:intergenic
TTGCAAAACTATGGACAATT+TGG 0.197366 1.3:+55603950 MS.gene07508:CDS
CATGATGTGGATGGTCTTCT+AGG 0.248249 1.3:-55603829 None:intergenic
AAGTGATTCAATTCTTATTA+GGG 0.263216 1.3:-55604177 None:intergenic
CTATGGACAATTTGGTTTCA+TGG 0.269872 1.3:+55603958 MS.gene07508:CDS
TGGTTTCATGGCTGTTAGTT+TGG 0.281840 1.3:+55603970 MS.gene07508:CDS
ATGAGCTATTTGCAAAACTA+TGG 0.283875 1.3:+55603941 MS.gene07508:CDS
GTATCTAGACTTCAAGAATA+TGG 0.292860 1.3:+55604151 MS.gene07508:CDS
AGGATGTCAATAGGGTATAT+TGG 0.322482 1.3:+55604091 MS.gene07508:CDS
TGTTGGTCCTAATAGCATTA+TGG 0.326242 1.3:-55603672 None:intergenic
TCTGAGGAAAAGGTTCTTGC+TGG 0.335620 1.3:+55604049 MS.gene07508:CDS
GGTTTCATGGCTGTTAGTTT+GGG 0.336700 1.3:+55603971 MS.gene07508:CDS
GAAACACTTATGTCTTGTTC+TGG 0.350339 1.3:+55604001 MS.gene07508:CDS
CAAGGGGCCATAATGCTATT+AGG 0.360521 1.3:+55603665 MS.gene07508:CDS
TTGGGACTTTGGAGCAGAAA+TGG 0.390914 1.3:+55604110 MS.gene07508:CDS
TCAATTCACAACAAAGACTA+TGG 0.391666 1.3:+55603863 MS.gene07508:CDS
TGCTATGTTCTTTCATCCTT+AGG 0.394998 1.3:-55603745 None:intergenic
GGATGTCAATAGGGTATATT+GGG 0.402478 1.3:+55604092 MS.gene07508:CDS
CACAACAAAGACTATGGTTA+TGG 0.412875 1.3:+55603869 MS.gene07508:CDS
GGTCTTGTGAGGATGTCAAT+AGG 0.421102 1.3:+55604082 MS.gene07508:CDS
AATAGGGTATATTGGGACTT+TGG 0.430260 1.3:+55604099 MS.gene07508:CDS
TGAGTTGAATTCTGAGGAAA+AGG 0.445451 1.3:+55604039 MS.gene07508:CDS
GTCTTGTGAGGATGTCAATA+GGG 0.477443 1.3:+55604083 MS.gene07508:CDS
CTAATAGCATTATGGCCCCT+TGG 0.496849 1.3:-55603664 None:intergenic
TGTGGATGGTCTTCTAGGCC+AGG 0.509408 1.3:-55603824 None:intergenic
GAAATGGAATCAGCTTGAAA+AGG 0.516409 1.3:+55604126 MS.gene07508:CDS
GAATCCCTCACTTAGGCCTA+AGG 0.525026 1.3:+55603729 MS.gene07508:CDS
GTTCTTGCTGGAATTTCACC+AGG 0.532752 1.3:+55604061 MS.gene07508:CDS
ATAGCATTATGGCCCCTTGG+TGG 0.543434 1.3:-55603661 None:intergenic
GTGGATGGTCTTCTAGGCCA+GGG 0.553045 1.3:-55603823 None:intergenic
GATTTCAACAATTCATGATG+TGG 0.554996 1.3:-55603842 None:intergenic
TCAACAATTCATGATGTGGA+TGG 0.562975 1.3:-55603838 None:intergenic
TGGACTTCTTTGCATTGACA+TGG 0.579725 1.3:+55603892 MS.gene07508:CDS
AACAAAGACTATGGTTATGG+TGG 0.583091 1.3:+55603872 MS.gene07508:CDS
CTATGATGGATCTCCACCAA+GGG 0.585796 1.3:+55603648 None:intergenic
ATTGACATCCTCACAAGACC+TGG 0.585919 1.3:-55604079 None:intergenic
GAATTTCACCAGGTCTTGTG+AGG 0.587396 1.3:+55604071 MS.gene07508:CDS
TCAGAAAGAATCCCTCACTT+AGG 0.590587 1.3:+55603722 MS.gene07508:CDS
GGATTCAAGGTAATCTACCC+TGG 0.598316 1.3:+55603806 MS.gene07508:CDS
TCATCCTTAGGCCTAAGTGA+GGG 0.616919 1.3:-55603733 None:intergenic
TTCATCCTTAGGCCTAAGTG+AGG 0.628773 1.3:-55603734 None:intergenic
TCTATGATGGATCTCCACCA+AGG 0.633733 1.3:+55603647 None:intergenic
TAGTAGTGAGTTGAATTCTG+AGG 0.646330 1.3:+55604033 MS.gene07508:CDS
TTGTTGCAAACACTAACGCA+CGG 0.672812 1.3:-55603769 None:intergenic
TATGATGGATCTCCACCAAG+GGG 0.715539 1.3:+55603649 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTAAAAAAATTAGGATTCA+AGG + chr1.3:55603793-55603812 MS.gene07508:CDS 20.0%
! GCAACAAGACTAAAAAAATT+AGG + chr1.3:55603785-55603804 MS.gene07508:CDS 25.0%
!!! ATAGTTCAAATGCTATTTTG+GGG - chr1.3:55603703-55603722 None:intergenic 25.0%
!!! GATAGTTCAAATGCTATTTT+GGG - chr1.3:55603704-55603723 None:intergenic 25.0%
!!! TGATAGTTCAAATGCTATTT+TGG - chr1.3:55603705-55603724 None:intergenic 25.0%
ATGAGCTATTTGCAAAACTA+TGG + chr1.3:55603941-55603960 MS.gene07508:CDS 30.0%
GATTTCAACAATTCATGATG+TGG - chr1.3:55603845-55603864 None:intergenic 30.0%
GTATCTAGACTTCAAGAATA+TGG + chr1.3:55604151-55604170 MS.gene07508:CDS 30.0%
TCAATTCACAACAAAGACTA+TGG + chr1.3:55603863-55603882 MS.gene07508:CDS 30.0%
TTGCAAAACTATGGACAATT+TGG + chr1.3:55603950-55603969 MS.gene07508:CDS 30.0%
AACAAAGACTATGGTTATGG+TGG + chr1.3:55603872-55603891 MS.gene07508:CDS 35.0%
AATAGGGTATATTGGGACTT+TGG + chr1.3:55604099-55604118 MS.gene07508:CDS 35.0%
AGGATGTCAATAGGGTATAT+TGG + chr1.3:55604091-55604110 MS.gene07508:CDS 35.0%
CACAACAAAGACTATGGTTA+TGG + chr1.3:55603869-55603888 MS.gene07508:CDS 35.0%
GAAATGGAATCAGCTTGAAA+AGG + chr1.3:55604126-55604145 MS.gene07508:CDS 35.0%
GGATGTCAATAGGGTATATT+GGG + chr1.3:55604092-55604111 MS.gene07508:CDS 35.0%
TCAACAATTCATGATGTGGA+TGG - chr1.3:55603841-55603860 None:intergenic 35.0%
TGCTATGTTCTTTCATCCTT+AGG - chr1.3:55603748-55603767 None:intergenic 35.0%
TGTTGGTCCTAATAGCATTA+TGG - chr1.3:55603675-55603694 None:intergenic 35.0%
! CTATGGACAATTTGGTTTCA+TGG + chr1.3:55603958-55603977 MS.gene07508:CDS 35.0%
! GAAACACTTATGTCTTGTTC+TGG + chr1.3:55604001-55604020 MS.gene07508:CDS 35.0%
! TAGTAGTGAGTTGAATTCTG+AGG + chr1.3:55604033-55604052 MS.gene07508:CDS 35.0%
! TGAGTTGAATTCTGAGGAAA+AGG + chr1.3:55604039-55604058 MS.gene07508:CDS 35.0%
GTCTTGTGAGGATGTCAATA+GGG + chr1.3:55604083-55604102 MS.gene07508:CDS 40.0%
TCAGAAAGAATCCCTCACTT+AGG + chr1.3:55603722-55603741 MS.gene07508:CDS 40.0%
TGGTTTCATGGCTGTTAGTT+TGG + chr1.3:55603970-55603989 MS.gene07508:CDS 40.0%
TTGTTGCAAACACTAACGCA+CGG - chr1.3:55603772-55603791 None:intergenic 40.0%
! GGTTTCATGGCTGTTAGTTT+GGG + chr1.3:55603971-55603990 MS.gene07508:CDS 40.0%
! TGGACTTCTTTGCATTGACA+TGG + chr1.3:55603892-55603911 MS.gene07508:CDS 40.0%
!!! GCTATTTTGGGGTTCATTGT+TGG - chr1.3:55603692-55603711 None:intergenic 40.0%
ATTGACATCCTCACAAGACC+TGG - chr1.3:55604082-55604101 None:intergenic 45.0%
CAAGGGGCCATAATGCTATT+AGG + chr1.3:55603665-55603684 MS.gene07508:CDS 45.0%
CATGATGTGGATGGTCTTCT+AGG - chr1.3:55603832-55603851 None:intergenic 45.0%
CTAATAGCATTATGGCCCCT+TGG - chr1.3:55603667-55603686 None:intergenic 45.0%
GGATTCAAGGTAATCTACCC+TGG + chr1.3:55603806-55603825 MS.gene07508:CDS 45.0%
GTTCTTGCTGGAATTTCACC+AGG + chr1.3:55604061-55604080 MS.gene07508:CDS 45.0%
TCATCCTTAGGCCTAAGTGA+GGG - chr1.3:55603736-55603755 None:intergenic 45.0%
TCTGAGGAAAAGGTTCTTGC+TGG + chr1.3:55604049-55604068 MS.gene07508:CDS 45.0%
TTCATCCTTAGGCCTAAGTG+AGG - chr1.3:55603737-55603756 None:intergenic 45.0%
! GAATTTCACCAGGTCTTGTG+AGG + chr1.3:55604071-55604090 MS.gene07508:CDS 45.0%
! GGTCTTGTGAGGATGTCAAT+AGG + chr1.3:55604082-55604101 MS.gene07508:CDS 45.0%
!! TTGGGACTTTGGAGCAGAAA+TGG + chr1.3:55604110-55604129 MS.gene07508:CDS 45.0%
ATAGCATTATGGCCCCTTGG+TGG - chr1.3:55603664-55603683 None:intergenic 50.0%
GAATCCCTCACTTAGGCCTA+AGG + chr1.3:55603729-55603748 MS.gene07508:CDS 50.0%
GTGGATGGTCTTCTAGGCCA+GGG - chr1.3:55603826-55603845 None:intergenic 55.0%
TGTGGATGGTCTTCTAGGCC+AGG - chr1.3:55603827-55603846 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 55603650 55604192 55603650 ID=MS.gene07508
chr1.3 mRNA 55603650 55604192 55603650 ID=MS.gene07508.t1;Parent=MS.gene07508
chr1.3 exon 55603650 55604192 55603650 ID=MS.gene07508.t1.exon1;Parent=MS.gene07508.t1
chr1.3 CDS 55603650 55604192 55603650 ID=cds.MS.gene07508.t1;Parent=MS.gene07508.t1
Gene Sequence

>MS.gene07508

ATGATGGATCTCCACCAAGGGGCCATAATGCTATTAGGACCAACAATGAACCCCAAAATAGCATTTGAACTATCAGAAAGAATCCCTCACTTAGGCCTAAGGATGAAAGAACATAGCAACCGTGCGTTAGTGTTTGCAACAAGACTAAAAAAATTAGGATTCAAGGTAATCTACCCTGGCCTAGAAGACCATCCACATCATGAATTGTTGAAATCAATTCACAACAAAGACTATGGTTATGGTGGACTTCTTTGCATTGACATGGAAACTGAAGAAAGAGCTAACAAATTGATGAGCTATTTGCAAAACTATGGACAATTTGGTTTCATGGCTGTTAGTTTGGGTTACTATGAAACACTTATGTCTTGTTCTGGAAGTAGCACTAGTAGTGAGTTGAATTCTGAGGAAAAGGTTCTTGCTGGAATTTCACCAGGTCTTGTGAGGATGTCAATAGGGTATATTGGGACTTTGGAGCAGAAATGGAATCAGCTTGAAAAGGCAGTATCTAGACTTCAAGAATATGGTTTCCCTAATAAGAATTGA

Protein sequence

>MS.gene07508.t1

MMDLHQGAIMLLGPTMNPKIAFELSERIPHLGLRMKEHSNRALVFATRLKKLGFKVIYPGLEDHPHHELLKSIHNKDYGYGGLLCIDMETEERANKLMSYLQNYGQFGFMAVSLGYYETLMSCSGSSTSSELNSEEKVLAGISPGLVRMSIGYIGTLEQKWNQLEKAVSRLQEYGFPNKN