AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene060244


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060244.t1 MTR_5g074290 96.875 160 5 0 1 160 1 160 1.02e-111 314
MS.gene060244.t1 MTR_3g031140 63.953 172 46 4 1 158 1 170 2.93e-66 199
MS.gene060244.t1 MTR_2g016150 42.778 180 80 5 1 157 1 180 9.07e-26 97.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060244.t1 AT2G30230 55.866 179 56 4 1 159 1 176 7.19e-58 178
MS.gene060244.t1 AT1G06980 57.310 171 58 3 1 159 1 168 7.13e-57 176
MS.gene060244.t1 AT1G29195 39.683 189 81 5 1 157 1 188 2.33e-34 119

Find 32 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCCGTATAGTTCATTTAAA+TGG 0.184956 5.4:-59927536 MS.gene060244:CDS
AGAACTTCCCCAGCTGAAAT+TGG 0.250090 5.4:+59927491 None:intergenic
GAATAGCTTAAGGTGTTGTT+TGG 0.353238 5.4:-59927591 MS.gene060244:CDS
GGTTTGGTTAGAACATGATT+TGG 0.374435 5.4:+59927458 None:intergenic
CGAACGACTCCTTCAGAGCT+TGG 0.393027 5.4:+59927437 None:intergenic
ACAGAAACATTGTGAGTCTA+AGG 0.406561 5.4:-59927219 MS.gene060244:CDS
AGCCATTTAAATGAACTATA+CGG 0.418439 5.4:+59927534 None:intergenic
GACTCCTTCAGAGCTTGGTT+TGG 0.436195 5.4:+59927442 None:intergenic
GGTTAGAACATGATTTGGAT+TGG 0.459615 5.4:+59927463 None:intergenic
TTCTCTGGTAACGATGTGGA+TGG 0.462955 5.4:+59927353 None:intergenic
CAATAATGGGGAATAGCTTA+AGG 0.463109 5.4:-59927601 None:intergenic
ATTACACGTCCAATTTCAGC+TGG 0.481683 5.4:-59927500 MS.gene060244:CDS
ATCGCCGGCATAGCCGTAGC+TGG 0.494180 5.4:-59927175 MS.gene060244:CDS
CAAGATGAGGTTGCCAGCTA+CGG 0.497711 5.4:+59927162 None:intergenic
GTTGCCAGCTACGGCTATGC+CGG 0.501915 5.4:+59927171 None:intergenic
CCGGCGAAAGGATGAGAATC+CGG 0.510917 5.4:+59927414 None:intergenic
CTTCTTGCTCTTCTCTTCAG+AGG 0.524828 5.4:+59927268 None:intergenic
CCGGATTCTCATCCTTTCGC+CGG 0.527436 5.4:-59927414 MS.gene060244:CDS
CTAACCAAACCAAGCTCTGA+AGG 0.531317 5.4:-59927446 MS.gene060244:CDS
AAAGCTCGAGGCGAGATCGC+CGG 0.545398 5.4:-59927190 MS.gene060244:CDS
TCTATGATACTTTCAAGATG+AGG 0.552754 5.4:+59927149 None:intergenic
GCTCTGAAGGAGTCGTTCGC+CGG 0.557154 5.4:-59927433 MS.gene060244:CDS
TGAGCTCAGTCTCCGGCGAA+AGG 0.557541 5.4:+59927402 None:intergenic
CCTCTCTTGAGCTCAGTCTC+CGG 0.558760 5.4:+59927395 None:intergenic
TCTCTGGTAACGATGTGGAT+GGG 0.571267 5.4:+59927354 None:intergenic
TTGGCTTGTGTTCTTCCATG+TGG 0.602379 5.4:-59927572 MS.gene060244:CDS
CCGGAGACTGAGCTCAAGAG+AGG 0.612896 5.4:-59927395 MS.gene060244:CDS
TACACGTCCAATTTCAGCTG+GGG 0.613892 5.4:-59927498 MS.gene060244:CDS
TTACACGTCCAATTTCAGCT+GGG 0.627095 5.4:-59927499 MS.gene060244:CDS
ATCAAGTCTAGTGCTCCACA+TGG 0.636272 5.4:+59927557 None:intergenic
CTAAGGAGATGAAAAGCTCG+AGG 0.669226 5.4:-59927202 MS.gene060244:CDS
GAGGGTCAGAAGAAGCGTCG+CGG 0.726452 5.4:-59927324 MS.gene060244:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGCCATTTAAATGAACTATA+CGG + chr5.4:59927201-59927220 None:intergenic 25.0%
! ATCCGTATAGTTCATTTAAA+TGG - chr5.4:59927196-59927215 MS.gene060244:CDS 25.0%
TCTATGATACTTTCAAGATG+AGG + chr5.4:59927586-59927605 None:intergenic 30.0%
ACAGAAACATTGTGAGTCTA+AGG - chr5.4:59927513-59927532 MS.gene060244:CDS 35.0%
CATCGTTACCAGAGAAAAAA+CGG - chr5.4:59927386-59927405 MS.gene060244:CDS 35.0%
! GGTTAGAACATGATTTGGAT+TGG + chr5.4:59927272-59927291 None:intergenic 35.0%
! GGTTTGGTTAGAACATGATT+TGG + chr5.4:59927277-59927296 None:intergenic 35.0%
!! GAATAGCTTAAGGTGTTGTT+TGG - chr5.4:59927141-59927160 MS.gene060244:CDS 35.0%
!!! TTTTTTCTCTGGTAACGATG+TGG + chr5.4:59927386-59927405 None:intergenic 35.0%
ATTACACGTCCAATTTCAGC+TGG - chr5.4:59927232-59927251 MS.gene060244:CDS 40.0%
GTTACCAGAGAAAAAACGGA+GGG - chr5.4:59927390-59927409 MS.gene060244:CDS 40.0%
TTACACGTCCAATTTCAGCT+GGG - chr5.4:59927233-59927252 MS.gene060244:CDS 40.0%
AGAACTTCCCCAGCTGAAAT+TGG + chr5.4:59927244-59927263 None:intergenic 45.0%
ATCAAGTCTAGTGCTCCACA+TGG + chr5.4:59927178-59927197 None:intergenic 45.0%
CGTTACCAGAGAAAAAACGG+AGG - chr5.4:59927389-59927408 MS.gene060244:CDS 45.0%
CTAAGGAGATGAAAAGCTCG+AGG - chr5.4:59927530-59927549 MS.gene060244:CDS 45.0%
CTTCTTGCTCTTCTCTTCAG+AGG + chr5.4:59927467-59927486 None:intergenic 45.0%
TACACGTCCAATTTCAGCTG+GGG - chr5.4:59927234-59927253 MS.gene060244:CDS 45.0%
! CTAACCAAACCAAGCTCTGA+AGG - chr5.4:59927286-59927305 MS.gene060244:CDS 45.0%
! TCTCTGGTAACGATGTGGAT+GGG + chr5.4:59927381-59927400 None:intergenic 45.0%
! TTCTCTGGTAACGATGTGGA+TGG + chr5.4:59927382-59927401 None:intergenic 45.0%
!! TTGGCTTGTGTTCTTCCATG+TGG - chr5.4:59927160-59927179 MS.gene060244:CDS 45.0%
CAAGATGAGGTTGCCAGCTA+CGG + chr5.4:59927573-59927592 None:intergenic 50.0%
GACTCCTTCAGAGCTTGGTT+TGG + chr5.4:59927293-59927312 None:intergenic 50.0%
!!! CTGACCCTCCGTTTTTTCTC+TGG + chr5.4:59927397-59927416 None:intergenic 50.0%
CCGGATTCTCATCCTTTCGC+CGG - chr5.4:59927318-59927337 MS.gene060244:CDS 55.0%
CCGGCGAAAGGATGAGAATC+CGG + chr5.4:59927321-59927340 None:intergenic 55.0%
CCTCTCTTGAGCTCAGTCTC+CGG + chr5.4:59927340-59927359 None:intergenic 55.0%
CGAACGACTCCTTCAGAGCT+TGG + chr5.4:59927298-59927317 None:intergenic 55.0%
AAAGCTCGAGGCGAGATCGC+CGG - chr5.4:59927542-59927561 MS.gene060244:CDS 60.0%
CCGGAGACTGAGCTCAAGAG+AGG - chr5.4:59927337-59927356 MS.gene060244:CDS 60.0%
GTTGCCAGCTACGGCTATGC+CGG + chr5.4:59927564-59927583 None:intergenic 60.0%
TGAGCTCAGTCTCCGGCGAA+AGG + chr5.4:59927333-59927352 None:intergenic 60.0%
!! GAGGGTCAGAAGAAGCGTCG+CGG - chr5.4:59927408-59927427 MS.gene060244:CDS 60.0%
!! GCTCTGAAGGAGTCGTTCGC+CGG - chr5.4:59927299-59927318 MS.gene060244:CDS 60.0%
ATCGCCGGCATAGCCGTAGC+TGG - chr5.4:59927557-59927576 MS.gene060244:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 59927136 59927618 59927136 ID=MS.gene060244
chr5.4 mRNA 59927136 59927618 59927136 ID=MS.gene060244.t1;Parent=MS.gene060244
chr5.4 exon 59927136 59927618 59927136 ID=MS.gene060244.t1.exon1;Parent=MS.gene060244.t1
chr5.4 CDS 59927136 59927618 59927136 ID=cds.MS.gene060244.t1;Parent=MS.gene060244.t1
Gene Sequence

>MS.gene060244

ATGGGGAATAGCTTAAGGTGTTGTTTGGCTTGTGTTCTTCCATGTGGAGCACTAGACTTGATCCGTATAGTTCATTTAAATGGCTATGTAGAAGAGATTACACGTCCAATTTCAGCTGGGGAAGTTCTTAAAGCCAATCCAAATCATGTTCTAACCAAACCAAGCTCTGAAGGAGTCGTTCGCCGGATTCTCATCCTTTCGCCGGAGACTGAGCTCAAGAGAGGAAGCATATACTTCTTGATCCCATCCACATCGTTACCAGAGAAAAAACGGAGGGTCAGAAGAAGCGTCGCGGAAAAAGATCTTGAAAGCAAGAAAGTATATTTCTCCTCTGAAGAGAAGAGCAAGAAGTGTGACGATGATAATAAATCATCGTCACAGAAACATTGTGAGTCTAAGGAGATGAAAAGCTCGAGGCGAGATCGCCGGCATAGCCGTAGCTGGCAACCTCATCTTGAAAGTATCATAGAAGATTTGTGTTAG

Protein sequence

>MS.gene060244.t1

MGNSLRCCLACVLPCGALDLIRIVHLNGYVEEITRPISAGEVLKANPNHVLTKPSSEGVVRRILILSPETELKRGSIYFLIPSTSLPEKKRRVRRSVAEKDLESKKVYFSSEEKSKKCDDDNKSSSQKHCESKEMKSSRRDRRHSRSWQPHLESIIEDLC