AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07514

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07514.t1 MTR_1g077990 95.782 403 17 0 1 403 1 403 0.0 788
MS.gene07514.t1 MTR_2g086530 57.895 342 139 3 10 348 6 345 4.91e-144 415
MS.gene07514.t1 MTR_2g086530 61.321 318 120 2 32 348 9 324 5.34e-143 412
MS.gene07514.t1 MTR_4g052970 58.567 321 132 1 30 349 28 348 1.98e-140 406
MS.gene07514.t1 MTR_4g084480 52.617 363 168 2 4 362 2 364 6.29e-140 405
MS.gene07514.t1 MTR_4g084480 52.617 363 168 2 4 362 2 364 7.56e-140 405
MS.gene07514.t1 MTR_4g084480 54.971 342 153 1 22 362 16 357 2.36e-139 403
MS.gene07514.t1 MTR_2g019345 51.479 338 160 3 13 347 4 340 9.24e-123 361
MS.gene07514.t1 MTR_4g010200 49.020 357 174 3 11 359 7 363 1.16e-118 351
MS.gene07514.t1 MTR_4g114850 51.097 319 154 2 32 348 27 345 7.88e-111 330
MS.gene07514.t1 MTR_4g114850 51.097 319 154 2 32 348 27 345 1.72e-110 329
MS.gene07514.t1 MTR_4g114850 51.097 319 154 2 32 348 27 345 1.96e-110 330
MS.gene07514.t1 MTR_1g056640 48.788 330 165 4 30 356 37 365 1.25e-102 313
MS.gene07514.t1 MTR_5g085580 49.697 330 162 4 26 352 20 348 9.49e-102 310
MS.gene07514.t1 MTR_3g116510 43.978 357 196 3 13 365 8 364 2.87e-100 306
MS.gene07514.t1 MTR_1g022160 46.273 322 170 3 31 349 24 345 5.03e-100 304
MS.gene07514.t1 MTR_1g022160 46.273 322 170 3 31 349 24 345 3.21e-99 304
MS.gene07514.t1 MTR_1g022160 46.273 322 170 3 31 349 24 345 3.59e-99 304
MS.gene07514.t1 MTR_1g022160 46.273 322 170 3 31 349 24 345 4.72e-99 304
MS.gene07514.t1 MTR_3g080410 47.164 335 173 4 24 355 19 352 1.90e-98 298
MS.gene07514.t1 MTR_1g007810 46.108 334 171 5 16 345 1 329 2.34e-98 298
MS.gene07514.t1 MTR_4g069940 42.416 356 201 3 13 364 9 364 6.29e-97 298
MS.gene07514.t1 MTR_6g088885 41.783 359 205 4 13 368 9 366 9.97e-93 288
MS.gene07514.t1 MTR_7g026340 44.308 325 172 7 26 347 18 336 2.41e-91 283
MS.gene07514.t1 MTR_8g012400 40.110 364 205 7 14 367 8 368 1.99e-85 268
MS.gene07514.t1 MTR_4g083500 45.260 327 173 6 24 347 37 360 3.26e-82 261
MS.gene07514.t1 MTR_7g053500 41.049 324 184 5 32 349 27 349 3.52e-81 257
MS.gene07514.t1 MTR_4g088155 37.260 365 218 6 12 366 2 365 6.87e-81 256
MS.gene07514.t1 MTR_8g102340 43.789 322 142 4 31 349 28 313 4.77e-80 254
MS.gene07514.t1 MTR_8g085720 39.466 337 198 4 31 362 26 361 4.14e-78 250
MS.gene07514.t1 MTR_3g083580 36.314 369 224 6 19 377 10 377 1.03e-75 244
MS.gene07514.t1 MTR_4g076440 37.685 337 198 7 13 349 5 329 3.82e-72 229
MS.gene07514.t1 MTR_4g132280 40.123 324 188 4 31 349 45 367 5.62e-72 235
MS.gene07514.t1 MTR_1g031900 40.432 324 181 5 31 346 24 343 1.04e-71 233
MS.gene07514.t1 MTR_1g031900 40.061 327 184 5 31 349 24 346 2.21e-71 233
MS.gene07514.t1 MTR_4g076490 40.122 329 186 7 21 349 87 404 4.47e-70 227
MS.gene07514.t1 MTR_3g065460 36.047 344 213 5 12 349 4 346 6.26e-70 228
MS.gene07514.t1 MTR_4g076255 39.198 324 185 6 26 349 20 331 9.78e-70 223
MS.gene07514.t1 MTR_5g078200 36.950 341 207 5 16 349 6 345 1.06e-69 232
MS.gene07514.t1 MTR_4g076430 37.092 337 200 8 13 349 5 329 9.30e-67 215
MS.gene07514.t1 MTR_5g078200 38.339 313 187 4 42 349 1 312 2.40e-66 222
MS.gene07514.t1 MTR_4g076470 37.685 337 198 8 13 349 5 329 2.71e-66 214
MS.gene07514.t1 MTR_7g081350 36.310 336 202 8 13 346 10 335 4.00e-66 214
MS.gene07514.t1 MTR_1g052430 39.039 333 194 6 27 357 28 353 4.31e-66 216
MS.gene07514.t1 MTR_4g076500 38.344 326 189 8 27 349 74 390 1.07e-65 214
MS.gene07514.t1 MTR_3g116510 45.652 230 124 1 137 365 1 230 2.49e-64 209
MS.gene07514.t1 MTR_2g034480 36.982 338 198 8 19 348 9 339 9.17e-64 209
MS.gene07514.t1 MTR_7g053500 40.071 282 162 5 98 374 17 296 4.06e-63 208
MS.gene07514.t1 MTR_7g081370 34.627 335 197 6 13 346 10 323 1.34e-62 204
MS.gene07514.t1 MTR_8g012400 40.727 275 155 5 98 367 17 288 1.36e-62 206
MS.gene07514.t1 MTR_4g076570 36.937 333 203 5 28 359 19 345 2.57e-62 205
MS.gene07514.t1 MTR_2g034470 37.386 329 195 8 24 349 23 343 1.37e-61 203
MS.gene07514.t1 MTR_2g034440 37.304 319 189 8 24 339 15 325 3.90e-60 198
MS.gene07514.t1 MTR_2g034470 37.143 315 187 8 38 349 2 308 7.70e-58 192
MS.gene07514.t1 MTR_2g027560 34.582 347 210 12 24 360 22 361 4.88e-54 187
MS.gene07514.t1 MTR_1g010120 34.493 345 205 13 13 346 9 343 2.32e-53 184
MS.gene07514.t1 MTR_7g085390 33.333 351 210 13 13 349 7 347 1.13e-52 183
MS.gene07514.t1 MTR_8g092070 33.711 353 213 12 11 349 9 354 1.34e-52 185
MS.gene07514.t1 MTR_6g032820 32.558 344 209 11 15 344 10 344 6.79e-51 177
MS.gene07514.t1 MTR_3g108600 34.670 349 191 13 8 346 8 329 1.63e-50 177
MS.gene07514.t1 MTR_4g112025 33.426 359 217 13 1 346 1 350 4.42e-50 177
MS.gene07514.t1 MTR_4g124440 34.085 355 212 14 6 347 3 348 7.14e-48 170
MS.gene07514.t1 MTR_1g053320 33.430 344 208 13 16 346 15 350 1.33e-46 167
MS.gene07514.t1 MTR_3g095050 33.945 327 200 12 31 349 34 352 2.00e-46 166
MS.gene07514.t1 MTR_5g081720 35.168 327 190 12 31 345 33 349 2.52e-42 153
MS.gene07514.t1 MTR_3g031770 29.275 345 228 10 24 360 20 356 3.19e-32 127
MS.gene07514.t1 MTR_4g134280 31.174 247 157 9 120 360 5 244 8.85e-29 116
MS.gene07514.t1 MTR_4g036775 40.909 132 65 3 247 369 80 207 5.04e-18 82.8
MS.gene07514.t1 MTR_4g036680 42.105 133 62 5 247 369 80 207 9.59e-18 82.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07514.t1 AT1G32860 60.448 402 151 4 1 396 1 400 1.44e-166 474
MS.gene07514.t1 AT5G42100 67.081 322 102 2 30 347 26 347 1.62e-160 458
MS.gene07514.t1 AT5G42100 67.081 322 102 2 30 347 26 347 2.86e-160 458
MS.gene07514.t1 AT2G27500 61.846 325 122 2 27 349 25 349 7.03e-150 430
MS.gene07514.t1 AT2G27500 61.846 325 122 2 27 349 25 349 1.10e-149 430
MS.gene07514.t1 AT4G18340 49.138 348 176 1 13 359 16 363 5.58e-130 379
MS.gene07514.t1 AT4G18340 49.138 348 176 1 13 359 16 363 6.86e-130 380
MS.gene07514.t1 AT1G30080 52.924 342 155 2 12 347 12 353 2.11e-129 378
MS.gene07514.t1 AT1G30080 52.924 342 155 2 12 347 12 353 6.02e-129 377
MS.gene07514.t1 AT2G27500 62.731 271 99 2 81 349 1 271 1.97e-123 360
MS.gene07514.t1 AT3G15800 53.495 329 150 3 32 357 44 372 4.76e-120 355
MS.gene07514.t1 AT2G26600 50.893 336 163 2 32 365 35 370 4.79e-114 339
MS.gene07514.t1 AT4G26830 46.027 365 192 4 16 377 10 372 2.44e-108 327
MS.gene07514.t1 AT4G26830 46.910 356 184 4 25 377 23 376 9.70e-107 323
MS.gene07514.t1 AT2G05790 46.094 384 193 6 30 410 24 396 3.37e-105 319
MS.gene07514.t1 AT5G42720 46.132 349 182 4 16 358 8 356 5.09e-104 315
MS.gene07514.t1 AT4G34480 47.399 346 178 3 14 355 7 352 8.57e-104 311
MS.gene07514.t1 AT5G24318 45.723 339 174 6 13 347 13 345 1.54e-102 309
MS.gene07514.t1 AT5G24318 45.723 339 174 6 13 347 13 345 3.42e-101 308
MS.gene07514.t1 AT5G24318 45.723 339 174 6 13 347 13 345 6.11e-101 308
MS.gene07514.t1 AT5G55180 47.239 326 168 4 31 353 27 351 8.91e-100 305
MS.gene07514.t1 AT5G55180 47.147 333 170 5 31 360 27 356 1.13e-99 305
MS.gene07514.t1 AT4G34480 46.765 340 177 3 14 349 7 346 1.64e-99 305
MS.gene07514.t1 AT2G16230 46.526 331 175 2 31 359 25 355 3.95e-99 305
MS.gene07514.t1 AT2G26600 51.481 270 130 1 97 365 7 276 2.08e-91 278
MS.gene07514.t1 AT3G07320 44.152 342 182 6 32 367 26 364 1.95e-90 281
MS.gene07514.t1 AT5G56590 44.720 322 175 3 31 349 26 347 2.07e-90 282
MS.gene07514.t1 AT4G29360 44.286 350 189 4 13 360 12 357 5.92e-87 274
MS.gene07514.t1 AT4G29360 45.000 340 181 4 23 360 32 367 5.03e-86 272
MS.gene07514.t1 AT4G14080 41.193 352 192 7 4 349 22 364 8.99e-85 267
MS.gene07514.t1 AT3G23770 41.525 354 195 6 21 366 30 379 1.44e-83 263
MS.gene07514.t1 AT2G01630 41.975 324 182 4 31 349 23 345 5.28e-82 257
MS.gene07514.t1 AT3G13560 42.633 319 177 4 31 344 26 343 1.03e-81 259
MS.gene07514.t1 AT3G13560 42.633 319 177 4 31 344 26 343 1.03e-81 259
MS.gene07514.t1 AT3G13560 42.633 319 177 4 31 344 26 343 1.03e-81 259
MS.gene07514.t1 AT3G13560 42.633 319 177 4 31 344 26 343 1.03e-81 259
MS.gene07514.t1 AT3G13560 42.633 319 177 4 31 344 26 343 1.03e-81 259
MS.gene07514.t1 AT2G01630 41.975 324 182 4 31 349 23 345 1.61e-81 257
MS.gene07514.t1 AT2G01630 41.975 324 182 4 31 349 23 345 1.25e-80 257
MS.gene07514.t1 AT1G11820 40.841 333 191 4 27 354 40 371 1.87e-78 248
MS.gene07514.t1 AT1G11820 40.854 328 188 4 27 349 40 366 5.58e-77 246
MS.gene07514.t1 AT1G11820 40.854 328 188 4 27 349 40 366 8.55e-77 247
MS.gene07514.t1 AT3G55430 39.286 364 207 10 19 377 18 372 3.47e-76 244
MS.gene07514.t1 AT2G39640 39.583 336 194 5 13 347 12 339 1.47e-74 242
MS.gene07514.t1 AT3G46570 44.272 323 168 10 31 347 27 343 2.65e-74 236
MS.gene07514.t1 AT3G61810 37.313 335 195 5 22 349 46 372 8.23e-72 230
MS.gene07514.t1 AT4G16260 36.888 347 208 6 13 356 4 342 2.05e-71 228
MS.gene07514.t1 AT4G34480 50.644 233 114 1 118 349 3 235 7.62e-71 228
MS.gene07514.t1 AT3G57270 39.875 321 182 7 31 348 27 339 1.91e-70 225
MS.gene07514.t1 AT1G66250 38.024 334 200 5 25 353 26 357 2.99e-68 224
MS.gene07514.t1 AT4G16260 36.875 320 193 5 38 356 2 313 2.04e-65 211
MS.gene07514.t1 AT5G20330 36.207 348 205 10 7 346 6 344 3.71e-64 209
MS.gene07514.t1 AT5G20340 38.110 328 190 9 23 346 35 353 1.29e-63 208
MS.gene07514.t1 AT5G24318 48.095 210 103 3 140 347 1 206 2.55e-63 206
MS.gene07514.t1 AT5G20390 36.494 348 208 9 3 346 5 343 2.32e-62 204
MS.gene07514.t1 AT1G77780 35.311 354 211 11 13 359 2 344 9.09e-60 198
MS.gene07514.t1 AT1G33220 36.280 328 188 8 22 346 25 334 1.95e-59 197
MS.gene07514.t1 AT3G57260 34.024 338 210 7 13 349 14 339 1.20e-57 192
MS.gene07514.t1 AT3G57260 34.024 338 210 7 13 349 30 355 1.59e-57 192
MS.gene07514.t1 AT3G57240 36.250 320 190 9 31 349 35 341 1.83e-55 186
MS.gene07514.t1 AT1G77790 32.857 350 222 8 1 346 1 341 3.93e-54 183
MS.gene07514.t1 AT5G58090 34.808 339 203 12 27 355 18 348 1.84e-51 179
MS.gene07514.t1 AT4G31140 32.857 350 218 12 6 346 3 344 2.01e-50 177
MS.gene07514.t1 AT1G64760 33.429 347 213 11 13 349 7 345 4.13e-48 171
MS.gene07514.t1 AT1G64760 33.429 347 213 11 13 349 7 345 4.13e-48 171
MS.gene07514.t1 AT5G18220 31.871 342 211 10 14 344 13 343 1.81e-46 166
MS.gene07514.t1 AT5G64790 31.579 342 218 11 17 349 14 348 3.28e-45 163
MS.gene07514.t1 AT4G17180 32.448 339 210 11 30 360 24 351 4.69e-45 162
MS.gene07514.t1 AT2G19440 32.277 347 213 12 17 349 3 341 5.77e-45 162
MS.gene07514.t1 AT2G19440 32.277 347 213 12 17 349 3 341 5.77e-45 162
MS.gene07514.t1 AT5G20560 32.628 331 211 9 11 338 15 336 6.99e-45 159
MS.gene07514.t1 AT3G55780 33.227 313 196 10 44 349 48 354 3.72e-44 158
MS.gene07514.t1 AT5G20870 33.146 356 203 16 13 346 12 354 1.01e-43 159
MS.gene07514.t1 AT3G24330 31.818 352 216 14 11 349 21 361 1.61e-43 159
MS.gene07514.t1 AT3G04010 32.378 349 210 12 14 348 16 352 2.47e-42 155
MS.gene07514.t1 AT5G58480 30.172 348 227 10 26 364 21 361 1.61e-39 147

Find 82 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCGGTTATTTGGCCATTTC+TGG 0.048168 1.2:+62634623 MS.gene07514:CDS
TTGTGACAGTGATTTGTTTA+TGG 0.189858 1.2:-62633917 None:intergenic
TTATCCTTACTTTGCTTATA+AGG 0.190578 1.2:+62634073 MS.gene07514:CDS
AACTTCTTCCTCCGGTTATT+TGG 0.209849 1.2:+62634613 MS.gene07514:CDS
CCAACTTCTGAGAGGAATTA+TGG 0.228803 1.2:+62634440 MS.gene07514:CDS
GGGAATCCTGTTTATAATCT+TGG 0.235486 1.2:+62634479 MS.gene07514:CDS
CAAGAAAGGTGAACCAGTTT+TGG 0.236223 1.2:-62634042 None:intergenic
GCGAAAGCTTTGAGAACTTT+TGG 0.247516 1.2:-62633660 None:intergenic
TCCATGCTTGAGCTTGTTTA+GGG 0.266792 1.2:-62633743 None:intergenic
ATCCATGCTTGAGCTTGTTT+AGG 0.272003 1.2:-62633744 None:intergenic
CACACTGCACTCATAGTTCT+TGG 0.288580 1.2:+62633891 MS.gene07514:CDS
TTGTTTAGGGTCTTTCATTT+TGG 0.300191 1.2:-62633730 None:intergenic
GGATCAACCATTCCTTGGTT+TGG 0.327470 1.2:-62634137 None:intergenic
TTAAGCTTTCAATCCAAAAC+TGG 0.338481 1.2:+62634029 MS.gene07514:CDS
TATTCTGCGCTTGGAGCATT+AGG 0.348295 1.2:+62634212 MS.gene07514:CDS
ACTTCTTATCCACCTTCTTC+CGG 0.351800 1.2:+62633966 MS.gene07514:CDS
TCGAATGATGGTGGTAATAA+TGG 0.352748 1.2:+62634554 MS.gene07514:CDS
GTGTGAACACTTTGCATTGC+TGG 0.359796 1.2:-62633873 None:intergenic
GAAAATGCGAGAAAGTATAA+TGG 0.372959 1.2:+62634314 MS.gene07514:CDS
TAATCTAACGATATTTGCTT+AGG 0.376376 1.2:-62634101 None:intergenic
CCGGTGCATATTTCTGAAAC+AGG 0.382721 1.2:+62634248 MS.gene07514:CDS
GGTGGTAATAATGGTACTAT+TGG 0.395208 1.2:+62634563 MS.gene07514:CDS
TAAATATTCAAATCAACTTC+AGG 0.401550 1.2:-62634380 None:intergenic
GCAACAAAATCTGAATCAAT+TGG 0.409686 1.2:+62633534 MS.gene07514:CDS
CAACACCGGTATAGAATTCA+TGG 0.427382 1.2:+62633683 MS.gene07514:CDS
AAAATGCGAGAAAGTATAAT+GGG 0.428212 1.2:+62634315 MS.gene07514:CDS
TCCAGAAATGGCCAAATAAC+CGG 0.432218 1.2:-62634624 None:intergenic
GACGCTGTTTATTCTGCGCT+TGG 0.433976 1.2:+62634203 MS.gene07514:CDS
TATATCAGAACGAAAAGTTC+CGG 0.437960 1.2:-62633985 None:intergenic
TGAAGTTGCAATAGCGGTGC+TGG 0.451814 1.2:-62633406 None:intergenic
TATGGATTGTTTAAACCTGA+TGG 0.453340 1.2:+62634458 MS.gene07514:CDS
ATAGAATTCATGGTAGGGCT+TGG 0.457900 1.2:+62633693 MS.gene07514:CDS
GAGAATCCAAGATTATAAAC+AGG 0.458638 1.2:-62634485 None:intergenic
CATTTCTGGAGCTACTTCAC+TGG 0.464021 1.2:+62634637 MS.gene07514:CDS
AATCTAACGATATTTGCTTA+GGG 0.465599 1.2:-62634100 None:intergenic
CAGTGAAGTAGCTCCAGAAA+TGG 0.468492 1.2:-62634636 None:intergenic
CCATAATTCCTCTCAGAAGT+TGG 0.473439 1.2:-62634440 None:intergenic
TTATAAACAGGATTCCCATC+AGG 0.480279 1.2:-62634473 None:intergenic
CATCCTCCAACTTCTTCCTC+CGG 0.488695 1.2:+62634605 MS.gene07514:CDS
TTTGGATTGAAAGCTTAAGA+TGG 0.495529 1.2:-62634024 None:intergenic
ATAAAGTTGAGTAGTAAGAA+AGG 0.495740 1.2:+62634344 MS.gene07514:CDS
TCGAGTCCTCCTTCGAATGA+TGG 0.500599 1.2:+62634542 MS.gene07514:CDS
GAACGAAAAGTTCCGGAAGA+AGG 0.505186 1.2:-62633978 None:intergenic
ATGGATTGTTTAAACCTGAT+GGG 0.510406 1.2:+62634459 MS.gene07514:CDS
TTCCTCTCAGAAGTTGGACC+AGG 0.511117 1.2:-62634434 None:intergenic
AATAATGGTACTATTGGATC+TGG 0.512155 1.2:+62634569 MS.gene07514:CDS
GGATAAGCGTTTATCAAGAA+AGG 0.513672 1.2:-62634056 None:intergenic
GTTTCAACCAAACCAAGGAA+TGG 0.532105 1.2:+62634130 MS.gene07514:CDS
TTATCCTTATAAGCAAAGTA+AGG 0.538288 1.2:-62634077 None:intergenic
TGCATATTTCTGAAACAGGT+TGG 0.538422 1.2:+62634252 MS.gene07514:CDS
CCCTACCATGAATTCTATAC+CGG 0.540456 1.2:-62633688 None:intergenic
CTCAAAGCTTTCGCCAACAC+CGG 0.542007 1.2:+62633669 MS.gene07514:CDS
AAATAACCGGAGGAAGAAGT+TGG 0.548180 1.2:-62634611 None:intergenic
GTAACCCTAATAAAATGCAT+TGG 0.548583 1.2:+62633606 MS.gene07514:CDS
CATGAATTCTATACCGGTGT+TGG 0.552551 1.2:-62633682 None:intergenic
ACCCTAAACAAGCTCAAGCA+TGG 0.553134 1.2:+62633742 MS.gene07514:CDS
CTGGAATGTAGAACGATCCA+TGG 0.556195 1.2:-62633387 None:intergenic
GGTTGGCCTTCGAAAGGAGA+TGG 0.560352 1.2:+62634269 MS.gene07514:CDS
GAGGAAGAAGTTGGAGGATG+CGG 0.566760 1.2:-62634602 None:intergenic
GAATCAATTGGAATAAACTA+CGG 0.568633 1.2:+62633546 MS.gene07514:CDS
GAAACAGGTTGGCCTTCGAA+AGG 0.569337 1.2:+62634263 MS.gene07514:CDS
ACAGCATGCTATCATAATGC+AGG 0.570006 1.2:-62634169 None:intergenic
AAAGGAGATGGAGATGAGGT+TGG 0.576671 1.2:+62634281 MS.gene07514:CDS
CTTAAGATGGAAGCAATGCA+TGG 0.582736 1.2:-62634011 None:intergenic
CGAAAAGTTCCGGAAGAAGG+TGG 0.586411 1.2:-62633975 None:intergenic
TCATCTCCATCTCCTTTCGA+AGG 0.594882 1.2:-62634275 None:intergenic
GAAGAAGTTGGAGGATGCGG+CGG 0.595408 1.2:-62634599 None:intergenic
CCGGTATAGAATTCATGGTA+GGG 0.598659 1.2:+62633688 MS.gene07514:CDS
TTTAATGAGAATTTGAAACC+TGG 0.603078 1.2:+62634416 MS.gene07514:CDS
ACCGGTATAGAATTCATGGT+AGG 0.606594 1.2:+62633687 MS.gene07514:CDS
TGACGATGAAGTTGCAATAG+CGG 0.607393 1.2:-62633412 None:intergenic
TAACCGGAGGAAGAAGTTGG+AGG 0.618276 1.2:-62634608 None:intergenic
CCTGTTTCAGAAATATGCAC+CGG 0.625689 1.2:-62634248 None:intergenic
TTATTACCACCATCATTCGA+AGG 0.630115 1.2:-62634551 None:intergenic
TTCGAAAGGAGATGGAGATG+AGG 0.634668 1.2:+62634277 MS.gene07514:CDS
AATGGTACTATTGGATCTGG+TGG 0.638028 1.2:+62634572 MS.gene07514:CDS
ATTAGGTTACGATAAAATGC+CGG 0.638184 1.2:+62634229 MS.gene07514:CDS
AAATCAACTTCAGGTCTCAA+CGG 0.641716 1.2:-62634371 None:intergenic
AGTCCTCCTTCGAATGATGG+TGG 0.666041 1.2:+62634545 MS.gene07514:CDS
TTACCACCATCATTCGAAGG+AGG 0.670183 1.2:-62634548 None:intergenic
AACCTGGTCCAACTTCTGAG+AGG 0.694615 1.2:+62634432 MS.gene07514:CDS
AGAAATGGCCAAATAACCGG+AGG 0.696188 1.2:-62634621 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATTATCATTTTCATTAC+AGG + chr1.2:62633434-62633453 MS.gene07514:CDS 15.0%
!! ACTATATAATAAGATGTAGT+AGG - chr1.2:62633474-62633493 None:intergenic 20.0%
!! TAAATATTCAAATCAACTTC+AGG - chr1.2:62634383-62634402 None:intergenic 20.0%
! AACTTCATTTCCTACAAAAA+TGG - chr1.2:62633814-62633833 None:intergenic 25.0%
! AATCTAACGATATTTGCTTA+GGG - chr1.2:62634103-62634122 None:intergenic 25.0%
! ATAAAGTTGAGTAGTAAGAA+AGG + chr1.2:62634344-62634363 MS.gene07514:CDS 25.0%
! GAATCAATTGGAATAAACTA+CGG + chr1.2:62633546-62633565 MS.gene07514:CDS 25.0%
! TAATCTAACGATATTTGCTT+AGG - chr1.2:62634104-62634123 None:intergenic 25.0%
! TTATCCTTACTTTGCTTATA+AGG + chr1.2:62634073-62634092 MS.gene07514:CDS 25.0%
! TTATCCTTATAAGCAAAGTA+AGG - chr1.2:62634080-62634099 None:intergenic 25.0%
!! AAAAATGGAAGTGATTTTTG+TGG - chr1.2:62633799-62633818 None:intergenic 25.0%
!! AAAATGCGAGAAAGTATAAT+GGG + chr1.2:62634315-62634334 MS.gene07514:CDS 25.0%
!! TTTAATGAGAATTTGAAACC+TGG + chr1.2:62634416-62634435 MS.gene07514:CDS 25.0%
!!! AAAATCACTTCCATTTTTGT+AGG + chr1.2:62633801-62633820 MS.gene07514:CDS 25.0%
AATAATGGTACTATTGGATC+TGG + chr1.2:62634569-62634588 MS.gene07514:CDS 30.0%
ATTAGGTTACGATAAAATGC+CGG + chr1.2:62634229-62634248 MS.gene07514:CDS 30.0%
GAGAATCCAAGATTATAAAC+AGG - chr1.2:62634488-62634507 None:intergenic 30.0%
GCAACAAAATCTGAATCAAT+TGG + chr1.2:62633534-62633553 MS.gene07514:CDS 30.0%
GTAACCCTAATAAAATGCAT+TGG + chr1.2:62633606-62633625 MS.gene07514:CDS 30.0%
TATATCAGAACGAAAAGTTC+CGG - chr1.2:62633988-62634007 None:intergenic 30.0%
TTAAGCTTTCAATCCAAAAC+TGG + chr1.2:62634029-62634048 MS.gene07514:CDS 30.0%
TTTGGATTGAAAGCTTAAGA+TGG - chr1.2:62634027-62634046 None:intergenic 30.0%
! ATGGATTGTTTAAACCTGAT+GGG + chr1.2:62634459-62634478 MS.gene07514:CDS 30.0%
! GAAAATGCGAGAAAGTATAA+TGG + chr1.2:62634314-62634333 MS.gene07514:CDS 30.0%
! TATGGATTGTTTAAACCTGA+TGG + chr1.2:62634458-62634477 MS.gene07514:CDS 30.0%
! TGCTCCAATGCATTTTATTA+GGG - chr1.2:62633613-62633632 None:intergenic 30.0%
! TTGCTCCAATGCATTTTATT+AGG - chr1.2:62633614-62633633 None:intergenic 30.0%
!! TCATAATGCAGGTTTGTTTT+TGG - chr1.2:62634161-62634180 None:intergenic 30.0%
!! TTGTGACAGTGATTTGTTTA+TGG - chr1.2:62633920-62633939 None:intergenic 30.0%
!! TTGTTTAGGGTCTTTCATTT+TGG - chr1.2:62633733-62633752 None:intergenic 30.0%
AAATCAACTTCAGGTCTCAA+CGG - chr1.2:62634374-62634393 None:intergenic 35.0%
ATATAGTGTCCTATTTGTGC+AGG + chr1.2:62633507-62633526 MS.gene07514:intron 35.0%
GGATAAGCGTTTATCAAGAA+AGG - chr1.2:62634059-62634078 None:intergenic 35.0%
GGGAATCCTGTTTATAATCT+TGG + chr1.2:62634479-62634498 MS.gene07514:CDS 35.0%
GGTGGTAATAATGGTACTAT+TGG + chr1.2:62634563-62634582 MS.gene07514:CDS 35.0%
TTATAAACAGGATTCCCATC+AGG - chr1.2:62634476-62634495 None:intergenic 35.0%
TTATTACCACCATCATTCGA+AGG - chr1.2:62634554-62634573 None:intergenic 35.0%
! ATGGAAGTGATTTTTGTGGA+AGG - chr1.2:62633795-62633814 None:intergenic 35.0%
! ATTAGGGTTACTACGTTTTC+TGG - chr1.2:62633597-62633616 None:intergenic 35.0%
! TGGAAGTGATTTTTGTGGAA+GGG - chr1.2:62633794-62633813 None:intergenic 35.0%
! TTTTTGGATCAACCATTCCT+TGG - chr1.2:62634145-62634164 None:intergenic 35.0%
!! TCGAATGATGGTGGTAATAA+TGG + chr1.2:62634554-62634573 MS.gene07514:CDS 35.0%
!! TGCATATTTCTGAAACAGGT+TGG + chr1.2:62634252-62634271 MS.gene07514:CDS 35.0%
!!! GTTTTGTTTCAACCAAACCA+AGG + chr1.2:62634125-62634144 MS.gene07514:CDS 35.0%
AAATAACCGGAGGAAGAAGT+TGG - chr1.2:62634614-62634633 None:intergenic 40.0%
AACTTCTTCCTCCGGTTATT+TGG + chr1.2:62634613-62634632 MS.gene07514:CDS 40.0%
AATGGTACTATTGGATCTGG+TGG + chr1.2:62634572-62634591 MS.gene07514:CDS 40.0%
ACAGCATGCTATCATAATGC+AGG - chr1.2:62634172-62634191 None:intergenic 40.0%
ACCGGTATAGAATTCATGGT+AGG + chr1.2:62633687-62633706 MS.gene07514:CDS 40.0%
ACTTCTTATCCACCTTCTTC+CGG + chr1.2:62633966-62633985 MS.gene07514:CDS 40.0%
ATAGAATTCATGGTAGGGCT+TGG + chr1.2:62633693-62633712 MS.gene07514:CDS 40.0%
CAACACCGGTATAGAATTCA+TGG + chr1.2:62633683-62633702 MS.gene07514:CDS 40.0%
CATGAATTCTATACCGGTGT+TGG - chr1.2:62633685-62633704 None:intergenic 40.0%
CCATAATTCCTCTCAGAAGT+TGG - chr1.2:62634443-62634462 None:intergenic 40.0%
CCCTACCATGAATTCTATAC+CGG - chr1.2:62633691-62633710 None:intergenic 40.0%
CCGGTATAGAATTCATGGTA+GGG + chr1.2:62633688-62633707 MS.gene07514:CDS 40.0%
CCTGTTTCAGAAATATGCAC+CGG - chr1.2:62634251-62634270 None:intergenic 40.0%
CTTAAGATGGAAGCAATGCA+TGG - chr1.2:62634014-62634033 None:intergenic 40.0%
GTTTCAACCAAACCAAGGAA+TGG + chr1.2:62634130-62634149 MS.gene07514:CDS 40.0%
TCCAGAAATGGCCAAATAAC+CGG - chr1.2:62634627-62634646 None:intergenic 40.0%
TGACGATGAAGTTGCAATAG+CGG - chr1.2:62633415-62633434 None:intergenic 40.0%
! AAGTGATTTTTGTGGAAGGG+AGG - chr1.2:62633791-62633810 None:intergenic 40.0%
! ATCCATGCTTGAGCTTGTTT+AGG - chr1.2:62633747-62633766 None:intergenic 40.0%
! ATTTTTGTGGAAGGGAGGTA+GGG - chr1.2:62633786-62633805 None:intergenic 40.0%
! CAAGAAAGGTGAACCAGTTT+TGG - chr1.2:62634045-62634064 None:intergenic 40.0%
! CCAACTTCTGAGAGGAATTA+TGG + chr1.2:62634440-62634459 MS.gene07514:CDS 40.0%
! TCCATGCTTGAGCTTGTTTA+GGG - chr1.2:62633746-62633765 None:intergenic 40.0%
!! GTTACTACGTTTTCTGGTGA+TGG - chr1.2:62633591-62633610 None:intergenic 40.0%
!!! GCGAAAGCTTTGAGAACTTT+TGG - chr1.2:62633663-62633682 None:intergenic 40.0%
AAAGGAGATGGAGATGAGGT+TGG + chr1.2:62634281-62634300 MS.gene07514:CDS 45.0%
ACCCTAAACAAGCTCAAGCA+TGG + chr1.2:62633742-62633761 MS.gene07514:CDS 45.0%
AGAAATGGCCAAATAACCGG+AGG - chr1.2:62634624-62634643 None:intergenic 45.0%
CACACTGCACTCATAGTTCT+TGG + chr1.2:62633891-62633910 MS.gene07514:CDS 45.0%
CAGTGAAGTAGCTCCAGAAA+TGG - chr1.2:62634639-62634658 None:intergenic 45.0%
GAACGAAAAGTTCCGGAAGA+AGG - chr1.2:62633981-62634000 None:intergenic 45.0%
GGATCAACCATTCCTTGGTT+TGG - chr1.2:62634140-62634159 None:intergenic 45.0%
GTGTGAACACTTTGCATTGC+TGG - chr1.2:62633876-62633895 None:intergenic 45.0%
GTTGCTAGTCCTGCACAAAT+AGG - chr1.2:62633519-62633538 None:intergenic 45.0%
TCATCTCCATCTCCTTTCGA+AGG - chr1.2:62634278-62634297 None:intergenic 45.0%
TCCGGTTATTTGGCCATTTC+TGG + chr1.2:62634623-62634642 MS.gene07514:CDS 45.0%
TTACCACCATCATTCGAAGG+AGG - chr1.2:62634551-62634570 None:intergenic 45.0%
TTCGAAAGGAGATGGAGATG+AGG + chr1.2:62634277-62634296 MS.gene07514:CDS 45.0%
! GATTTTTGTGGAAGGGAGGT+AGG - chr1.2:62633787-62633806 None:intergenic 45.0%
! TATTCTGCGCTTGGAGCATT+AGG + chr1.2:62634212-62634231 MS.gene07514:CDS 45.0%
!! CATTTCTGGAGCTACTTCAC+TGG + chr1.2:62634637-62634656 MS.gene07514:CDS 45.0%
!! CCGGTGCATATTTCTGAAAC+AGG + chr1.2:62634248-62634267 MS.gene07514:CDS 45.0%
!!! CTACGTTTTCTGGTGATGGT+AGG - chr1.2:62633587-62633606 None:intergenic 45.0%
AACCTGGTCCAACTTCTGAG+AGG + chr1.2:62634432-62634451 MS.gene07514:CDS 50.0%
AGTCCTCCTTCGAATGATGG+TGG + chr1.2:62634545-62634564 MS.gene07514:CDS 50.0%
CATCCTCCAACTTCTTCCTC+CGG + chr1.2:62634605-62634624 MS.gene07514:CDS 50.0%
CGAAAAGTTCCGGAAGAAGG+TGG - chr1.2:62633978-62633997 None:intergenic 50.0%
CTCAAAGCTTTCGCCAACAC+CGG + chr1.2:62633669-62633688 MS.gene07514:CDS 50.0%
GACGCTGTTTATTCTGCGCT+TGG + chr1.2:62634203-62634222 MS.gene07514:CDS 50.0%
GAGGAAGAAGTTGGAGGATG+CGG - chr1.2:62634605-62634624 None:intergenic 50.0%
TAACCGGAGGAAGAAGTTGG+AGG - chr1.2:62634611-62634630 None:intergenic 50.0%
TCGAGTCCTCCTTCGAATGA+TGG + chr1.2:62634542-62634561 MS.gene07514:CDS 50.0%
TGAAGTTGCAATAGCGGTGC+TGG - chr1.2:62633409-62633428 None:intergenic 50.0%
TTCCTCTCAGAAGTTGGACC+AGG - chr1.2:62634437-62634456 None:intergenic 50.0%
!! GAAACAGGTTGGCCTTCGAA+AGG + chr1.2:62634263-62634282 MS.gene07514:CDS 50.0%
GAAGAAGTTGGAGGATGCGG+CGG - chr1.2:62634602-62634621 None:intergenic 55.0%
!! GGTTGGCCTTCGAAAGGAGA+TGG + chr1.2:62634269-62634288 MS.gene07514:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 62633389 62634694 62633389 ID=MS.gene07514
chr1.2 mRNA 62633389 62634694 62633389 ID=MS.gene07514.t1;Parent=MS.gene07514
chr1.2 exon 62633389 62633455 62633389 ID=MS.gene07514.t1.exon1;Parent=MS.gene07514.t1
chr1.2 CDS 62633389 62633455 62633389 ID=cds.MS.gene07514.t1;Parent=MS.gene07514.t1
chr1.2 exon 62633529 62634694 62633529 ID=MS.gene07514.t1.exon2;Parent=MS.gene07514.t1
chr1.2 CDS 62633529 62634694 62633529 ID=cds.MS.gene07514.t1;Parent=MS.gene07514.t1
Gene Sequence

>MS.gene07514

ATGGATCGTTCTACATTCCAGCACCGCTATTGCAACTTCATCGTCATTATTATCATTTTCATTACAGGTAAAAATTGCTACTCCTACTACATCTTATTATATAGTCTTAAAATGTAACATATAGTGTCCTATTTGTGCAGGACTAGCAACAAAATCTGAATCAATTGGAATAAACTACGGTCAAATAGCAAACAACCTACCATCACCAGAAAACGTAGTAACCCTAATAAAATGCATTGGAGCAACAAAAGTAAAACTCTACGACGCAGATCCAAAAGTTCTCAAAGCTTTCGCCAACACCGGTATAGAATTCATGGTAGGGCTTGGAAATGAATATCTCTCCAAAATGAAAGACCCTAAACAAGCTCAAGCATGGATCAAAACAAATCTTCAACCCTACCTCCCTTCCACAAAAATCACTTCCATTTTTGTAGGAAATGAAGTTCTCACTTTCAATGACTCTTCTTTAACATCTTCTCTTCTACCAGCAATGCAAAGTGTTCACACTGCACTCATAGTTCTTGGTCTCCATAAACAAATCACTGTCACAACAACTCATTCATTAGCTATACTACAAACTTCTTATCCACCTTCTTCCGGAACTTTTCGTTCTGATATAGCTCCATGCATTGCTTCCATCTTAAGCTTTCAATCCAAAACTGGTTCACCTTTCTTGATAAACGCTTATCCTTACTTTGCTTATAAGGATAACCCTAAGCAAATATCGTTAGATTATGTTTTGTTTCAACCAAACCAAGGAATGGTTGATCCAAAAACAAACCTGCATTATGATAGCATGCTGTTTGCGCAGATCGACGCTGTTTATTCTGCGCTTGGAGCATTAGGTTACGATAAAATGCCGGTGCATATTTCTGAAACAGGTTGGCCTTCGAAAGGAGATGGAGATGAGGTTGGTGCTAATGTTGAAAATGCGAGAAAGTATAATGGGAATGTGATAAAGTTGAGTAGTAAGAAAGGAACACCGTTGAGACCTGAAGTTGATTTGAATATTTATGTTTTTGCACTTTTTAATGAGAATTTGAAACCTGGTCCAACTTCTGAGAGGAATTATGGATTGTTTAAACCTGATGGGAATCCTGTTTATAATCTTGGATTCTCGCTGTCTTCGTCTTCTTCTTCGTCTTCTTCGAATTCGAGTCCTCCTTCGAATGATGGTGGTAATAATGGTACTATTGGATCTGGTGGTGCTCCGCCGCATCCTCCAACTTCTTCCTCCGGTTATTTGGCCATTTCTGGAGCTACTTCACTGGTTAGTAACACTCACACTGCTGCTTCTAAGTTTTGA
Protein sequence

>MS.gene07514.t1

MDRSTFQHRYCNFIVIIIIFITGLATKSESIGINYGQIANNLPSPENVVTLIKCIGATKVKLYDADPKVLKAFANTGIEFMVGLGNEYLSKMKDPKQAQAWIKTNLQPYLPSTKITSIFVGNEVLTFNDSSLTSSLLPAMQSVHTALIVLGLHKQITVTTTHSLAILQTSYPPSSGTFRSDIAPCIASILSFQSKTGSPFLINAYPYFAYKDNPKQISLDYVLFQPNQGMVDPKTNLHYDSMLFAQIDAVYSALGALGYDKMPVHISETGWPSKGDGDEVGANVENARKYNGNVIKLSSKKGTPLRPEVDLNIYVFALFNENLKPGPTSERNYGLFKPDGNPVYNLGFSLSSSSSSSSSNSSPPSNDGGNNGTIGSGGAPPHPPTSSSGYLAISGATSLVSNTHTAASKF