AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07575

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07575.t1 MTR_1g079340 87.037 108 14 0 1 108 40 147 1.41e-63 196
MS.gene07575.t1 MTR_1g079350 64.815 108 33 1 1 108 1 103 6.88e-41 134
MS.gene07575.t1 MTR_1g079350 64.815 108 33 1 1 108 1 103 3.03e-40 134
MS.gene07575.t1 MTR_1g079180 62.963 108 35 1 1 108 1 103 1.99e-39 132
MS.gene07575.t1 MTR_1g079250 62.857 105 34 1 1 105 1 100 1.04e-38 131
MS.gene07575.t1 MTR_3g006850 64.151 106 33 1 3 108 16 116 6.23e-38 130
MS.gene07575.t1 MTR_1g100597 62.037 108 33 2 1 108 1 100 1.17e-37 128
MS.gene07575.t1 MTR_1g079260 59.259 108 36 2 1 108 1 100 1.23e-35 123
MS.gene07575.t1 MTR_1g079200 61.111 108 33 2 1 108 1 99 1.75e-35 122
MS.gene07575.t1 MTR_1g079370 60.185 108 35 2 1 108 1 100 2.64e-35 122
MS.gene07575.t1 MTR_1g079460 58.333 108 40 1 1 108 1 103 3.55e-35 121
MS.gene07575.t1 MTR_1g079850 60.550 109 37 2 1 108 1 104 9.86e-34 118
MS.gene07575.t1 MTR_1g079230 56.481 108 39 2 1 108 1 100 1.87e-32 114
MS.gene07575.t1 MTR_2g042540 55.963 109 40 3 1 108 1 102 1.00e-31 108
MS.gene07575.t1 MTR_1g026510 54.630 108 44 1 1 108 1 103 5.45e-31 111
MS.gene07575.t1 MTR_3g450990 54.286 105 38 2 4 108 10 104 6.27e-28 103
MS.gene07575.t1 MTR_3g452600 53.333 105 39 2 4 108 10 104 2.15e-27 101
MS.gene07575.t1 MTR_2g079100 53.704 108 32 3 3 108 8 99 2.39e-27 101
MS.gene07575.t1 MTR_1g100613 57.447 94 31 3 15 108 1 85 2.27e-25 95.9
MS.gene07575.t1 MTR_7g096300 54.128 109 38 4 1 106 1 100 9.58e-25 97.4
MS.gene07575.t1 MTR_1g079400 50.943 106 40 4 7 108 15 112 1.48e-24 94.4
MS.gene07575.t1 MTR_1g079380 56.481 108 39 2 1 108 1 100 2.33e-24 94.4
MS.gene07575.t1 MTR_1g078460 57.647 85 33 1 1 85 64 145 4.75e-24 90.1
MS.gene07575.t1 MTR_1g079410 48.182 110 45 4 3 108 7 108 1.55e-23 91.7
MS.gene07575.t1 MTR_1g079140 44.954 109 48 3 4 108 13 113 1.14e-19 82.0
MS.gene07575.t1 MTR_7g112550 43.137 102 52 2 7 108 10 105 1.19e-17 76.3
MS.gene07575.t1 MTR_1g083580 38.532 109 56 4 3 108 7 107 1.85e-16 72.8
MS.gene07575.t1 MTR_4g023570 36.893 103 57 2 7 109 4 98 5.89e-14 65.5
MS.gene07575.t1 MTR_7g112560 35.294 102 58 2 7 108 4 97 9.48e-12 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07575.t1 AT2G02340 43.810 105 50 4 1 105 33 128 2.34e-19 80.9
MS.gene07575.t1 AT5G24560 40.777 103 50 3 4 106 3 94 2.80e-18 76.3
MS.gene07575.t1 AT2G02320 40.777 103 50 4 3 105 39 130 7.93e-18 77.0
MS.gene07575.t1 AT2G02240 43.689 103 47 4 4 106 61 152 8.89e-18 77.0
MS.gene07575.t1 AT2G02230 37.143 105 55 3 4 108 32 125 2.27e-16 73.2
MS.gene07575.t1 AT1G80110 40.000 105 52 3 4 108 1 94 3.53e-16 72.0
MS.gene07575.t1 AT2G02250 39.806 103 51 4 4 106 47 138 4.56e-16 72.0
MS.gene07575.t1 AT2G02300 41.346 104 49 4 3 105 33 125 7.55e-16 71.2
MS.gene07575.t1 AT1G09155 40.196 102 53 2 7 108 3 96 2.64e-15 69.7
MS.gene07575.t1 AT1G56250 44.118 102 49 2 7 108 4 97 4.05e-15 69.3
MS.gene07575.t1 AT1G56240 45.631 103 46 3 7 108 4 97 6.26e-15 68.9
MS.gene07575.t1 AT2G02360 37.255 102 53 3 4 105 14 104 1.94e-13 64.7
MS.gene07575.t1 AT2G02350 37.500 104 54 4 3 106 23 115 2.77e-13 64.3
MS.gene07575.t1 AT2G02310 33.981 103 57 3 4 106 45 136 5.32e-13 63.5

Find 30 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTACGAATGTTGGCAAAGTT+AGG 0.391592 1.2:-62084747 None:intergenic
GGGAGTCGTACGAGAGAGTA+TGG 0.404280 1.2:-62084601 None:intergenic
TGAAATGTAGAGCTTTCAAT+TGG 0.411607 1.2:+62084919 MS.gene07575:intron
ATGATAGTCTCGATGAAATC+AGG 0.419415 1.2:-62084714 None:intergenic
CGTAGGCGGAGATGATGATA+TGG 0.454187 1.2:-62084803 None:intergenic
GGGTTCGTTTGATGAATTAC+CGG 0.460846 1.2:+62084563 MS.gene07575:CDS
GCTCCATACGGCATTGGAAT+CGG 0.461036 1.2:-62084673 None:intergenic
ATGGCGGCAACGCACTCTTC+CGG 0.468110 1.2:-62084582 None:intergenic
GGATAGTCTAACAGCGTCAA+CGG 0.493419 1.2:-62084622 None:intergenic
CCGCCGATTCCAATGCCGTA+TGG 0.519825 1.2:+62084670 MS.gene07575:CDS
GATAGTCTAACAGCGTCAAC+GGG 0.530764 1.2:-62084621 None:intergenic
CATTGGAATCGGCGGCAGAA+AGG 0.531956 1.2:-62084662 None:intergenic
AAAGTGGCTCCATACGGCAT+TGG 0.537349 1.2:-62084679 None:intergenic
TCGAGCATTGTCGTCCTCGT+AGG 0.541999 1.2:-62084820 None:intergenic
CAGGATGGGAAGGGATAAAG+TGG 0.575324 1.2:-62084695 None:intergenic
TCGATGAAATCAGGATGGGA+AGG 0.585459 1.2:-62084705 None:intergenic
TGATGATATGGCGATCACAC+AGG 0.589214 1.2:-62084791 None:intergenic
CGAGGACGACAATGCTCGAA+AGG 0.589885 1.2:+62084824 MS.gene07575:CDS
TCTAGCAGAAAGCATTGTAA+CGG 0.592424 1.2:-62084961 None:intergenic
AGGGATAAAGTGGCTCCATA+CGG 0.594900 1.2:-62084685 None:intergenic
AGCATTGTCGTCCTCGTAGG+CGG 0.601185 1.2:-62084817 None:intergenic
TAGTCTCGATGAAATCAGGA+TGG 0.606539 1.2:-62084710 None:intergenic
GATGATATGGCGATCACACA+GGG 0.607844 1.2:-62084790 None:intergenic
CGATGAAATCAGGATGGGAA+GGG 0.621372 1.2:-62084704 None:intergenic
AAAGGAATGTCTTCGAAACG+AGG 0.627030 1.2:-62084644 None:intergenic
CCATACGGCATTGGAATCGG+CGG 0.633498 1.2:-62084670 None:intergenic
AGTCTCGATGAAATCAGGAT+GGG 0.653231 1.2:-62084709 None:intergenic
AAGGAATGTCTTCGAAACGA+GGG 0.666047 1.2:-62084643 None:intergenic
TATCATCATCTCCGCCTACG+AGG 0.697798 1.2:+62084806 MS.gene07575:CDS
AGTCGTACGAGAGAGTATGG+CGG 0.784395 1.2:-62084598 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AACAATCAATTCATTTCAAC+AGG - chr1.2:62084869-62084888 None:intergenic 25.0%
! TTTCAATTGGATAAAAAGAG+TGG + chr1.2:62084932-62084951 MS.gene07575:CDS 25.0%
AAGCTCTACATTTCAACATA+AGG - chr1.2:62084914-62084933 None:intergenic 30.0%
AATCAATTCATTTCAACAGG+AGG - chr1.2:62084866-62084885 None:intergenic 30.0%
ATCAATTCATTTCAACAGGA+GGG - chr1.2:62084865-62084884 None:intergenic 30.0%
TGAAATGTAGAGCTTTCAAT+TGG + chr1.2:62084919-62084938 MS.gene07575:intron 30.0%
ATGATAGTCTCGATGAAATC+AGG - chr1.2:62084717-62084736 None:intergenic 35.0%
!!! AGCTTTTTTGGTACGAATGT+TGG - chr1.2:62084760-62084779 None:intergenic 35.0%
!!! GGCAAGATAAACAGCTTTTT+TGG - chr1.2:62084772-62084791 None:intergenic 35.0%
AAAGGAATGTCTTCGAAACG+AGG - chr1.2:62084647-62084666 None:intergenic 40.0%
AAGGAATGTCTTCGAAACGA+GGG - chr1.2:62084646-62084665 None:intergenic 40.0%
AGTCTCGATGAAATCAGGAT+GGG - chr1.2:62084712-62084731 None:intergenic 40.0%
GGGTTCGTTTGATGAATTAC+CGG + chr1.2:62084563-62084582 MS.gene07575:CDS 40.0%
GTACGAATGTTGGCAAAGTT+AGG - chr1.2:62084750-62084769 None:intergenic 40.0%
TAGTCTCGATGAAATCAGGA+TGG - chr1.2:62084713-62084732 None:intergenic 40.0%
TTCATTTCAACAGGAGGGTT+AGG - chr1.2:62084860-62084879 None:intergenic 40.0%
AGGGATAAAGTGGCTCCATA+CGG - chr1.2:62084688-62084707 None:intergenic 45.0%
CGATGAAATCAGGATGGGAA+GGG - chr1.2:62084707-62084726 None:intergenic 45.0%
GATAGTCTAACAGCGTCAAC+GGG - chr1.2:62084624-62084643 None:intergenic 45.0%
GATGATATGGCGATCACACA+GGG - chr1.2:62084793-62084812 None:intergenic 45.0%
GGATAGTCTAACAGCGTCAA+CGG - chr1.2:62084625-62084644 None:intergenic 45.0%
TCGATGAAATCAGGATGGGA+AGG - chr1.2:62084708-62084727 None:intergenic 45.0%
TGATGATATGGCGATCACAC+AGG - chr1.2:62084794-62084813 None:intergenic 45.0%
AAAGTGGCTCCATACGGCAT+TGG - chr1.2:62084682-62084701 None:intergenic 50.0%
CAGGATGGGAAGGGATAAAG+TGG - chr1.2:62084698-62084717 None:intergenic 50.0%
CGTAGGCGGAGATGATGATA+TGG - chr1.2:62084806-62084825 None:intergenic 50.0%
TATCATCATCTCCGCCTACG+AGG + chr1.2:62084806-62084825 MS.gene07575:CDS 50.0%
! AGTCGTACGAGAGAGTATGG+CGG - chr1.2:62084601-62084620 None:intergenic 50.0%
! GCTCCATACGGCATTGGAAT+CGG - chr1.2:62084676-62084695 None:intergenic 50.0%
AGCATTGTCGTCCTCGTAGG+CGG - chr1.2:62084820-62084839 None:intergenic 55.0%
CATTGGAATCGGCGGCAGAA+AGG - chr1.2:62084665-62084684 None:intergenic 55.0%
CGAGGACGACAATGCTCGAA+AGG + chr1.2:62084824-62084843 MS.gene07575:CDS 55.0%
GGGAGTCGTACGAGAGAGTA+TGG - chr1.2:62084604-62084623 None:intergenic 55.0%
! CCATACGGCATTGGAATCGG+CGG - chr1.2:62084673-62084692 None:intergenic 55.0%
! TCGAGCATTGTCGTCCTCGT+AGG - chr1.2:62084823-62084842 None:intergenic 55.0%
ATGGCGGCAACGCACTCTTC+CGG - chr1.2:62084585-62084604 None:intergenic 60.0%
!! CCGCCGATTCCAATGCCGTA+TGG + chr1.2:62084670-62084689 MS.gene07575:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 62084561 62084982 62084561 ID=MS.gene07575
chr1.2 mRNA 62084561 62084982 62084561 ID=MS.gene07575.t1;Parent=MS.gene07575
chr1.2 exon 62084561 62084845 62084561 ID=MS.gene07575.t1.exon1;Parent=MS.gene07575.t1
chr1.2 CDS 62084561 62084845 62084561 ID=cds.MS.gene07575.t1;Parent=MS.gene07575.t1
chr1.2 exon 62084929 62084982 62084929 ID=MS.gene07575.t1.exon2;Parent=MS.gene07575.t1
chr1.2 CDS 62084929 62084982 62084929 ID=cds.MS.gene07575.t1;Parent=MS.gene07575.t1
Gene Sequence

>MS.gene07575

ATGGGTTCGTTTGATGAATTACCGGAAGAGTGCGTTGCCGCCATACTCTCTCGTACGACTCCCGTTGACGCTGTTAGACTATCCCTCGTTTCGAAGACATTCCTTTCTGCCGCCGATTCCAATGCCGTATGGAGCCACTTTATCCCTTCCCATCCTGATTTCATCGAGACTATCATCTCAAATTCTCCTAACTTTGCCAACATTCGTACCAAAAAAGCTGTTTATCTTGCCCTGTGTGATCGCCATATCATCATCTCCGCCTACGAGGACGACAATGCTCGAAAGGTAATGTTTTTCCTAACCCTCCTGTTGAAATGAATTGATTGTTGTATAAAATGCTAACGACAATTCCTTATGTTGAAATGTAGAGCTTTCAATTGGATAAAAAGAGTGGAAAAAACCGTTACAATGCTTTCTGCTAG
Protein sequence

>MS.gene07575.t1

MGSFDELPEECVAAILSRTTPVDAVRLSLVSKTFLSAADSNAVWSHFIPSHPDFIETIISNSPNFANIRTKKAVYLALCDRHIIISAYEDDNARKSFQLDKKSGKNRYNAFC