AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene057452


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057452.t1 MTR_7g013700 82.812 64 10 1 18 81 559 621 4.28e-20 83.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 23 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGGGCTCAAAAGCCTTTC+TGG 0.117481 7.2:+83661192 MS.gene057452:CDS
CCTTGCCCCACCCAAGCAAT+TGG 0.244168 7.2:+83661174 MS.gene057452:CDS
TCAGTTGAATCATAAAATTT+AGG 0.266358 7.2:-83660812 None:intergenic
AATTTAGGTGGAGGAGGTGG+TGG 0.310202 7.2:-83660797 None:intergenic
AGATACTTTAGTCAAGCTAA+TGG 0.358969 7.2:+83661092 MS.gene057452:intron
CTTGCCCCACCCAAGCAATT+GGG 0.378546 7.2:+83661175 MS.gene057452:CDS
TTAGTCAAGCTAATGGAATA+TGG 0.379441 7.2:+83661099 MS.gene057452:CDS
TTGGGCTCAAAAGCCTTTCT+GGG 0.382975 7.2:+83661193 MS.gene057452:CDS
ACAAAATCTGATGCAGTTCC+TGG 0.384698 7.2:+83660872 MS.gene057452:CDS
AAGACAAGAGCAATAGTTTA+CGG 0.386481 7.2:+83660846 MS.gene057452:CDS
TTAGGTGGAGGAGGTGGTGG+GGG 0.386881 7.2:-83660794 None:intergenic
TTTAGGTGGAGGAGGTGGTG+GGG 0.401933 7.2:-83660795 None:intergenic
TTGAGCCCAATTGCTTGGGT+GGG 0.419719 7.2:-83661180 None:intergenic
TTTGAGCCCAATTGCTTGGG+TGG 0.431489 7.2:-83661181 None:intergenic
TGAGCCCAATTGCTTGGGTG+GGG 0.436460 7.2:-83661179 None:intergenic
CCAATTGCTTGGGTGGGGCA+AGG 0.495513 7.2:-83661174 None:intergenic
ATTTAGGTGGAGGAGGTGGT+GGG 0.510960 7.2:-83660796 None:intergenic
TAAAATTTAGGTGGAGGAGG+TGG 0.516302 7.2:-83660800 None:intergenic
TCATAAAATTTAGGTGGAGG+AGG 0.523877 7.2:-83660803 None:intergenic
GAATCATAAAATTTAGGTGG+AGG 0.526336 7.2:-83660806 None:intergenic
GTTGAATCATAAAATTTAGG+TGG 0.536412 7.2:-83660809 None:intergenic
ACAGATCATAAAGCCCAGAA+AGG 0.597506 7.2:-83661206 None:intergenic
ATTGCTTGGGTGGGGCAAGG+AGG 0.609227 7.2:-83661171 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAGTTGAATCATAAAATTT+AGG - chr7.2:83660815-83660834 None:intergenic 20.0%
!!! TTAGATTTGTTTGTAACTTT+AGG + chr7.2:83661059-83661078 MS.gene057452:intron 20.0%
! AGCATTACAAAGTCAATTAA+AGG - chr7.2:83661021-83661040 None:intergenic 25.0%
! GCATTACAAAGTCAATTAAA+GGG - chr7.2:83661020-83661039 None:intergenic 25.0%
! GTTGAATCATAAAATTTAGG+TGG - chr7.2:83660812-83660831 None:intergenic 25.0%
AAGACAAGAGCAATAGTTTA+CGG + chr7.2:83660846-83660865 MS.gene057452:CDS 30.0%
AGATACTTTAGTCAAGCTAA+TGG + chr7.2:83661092-83661111 MS.gene057452:intron 30.0%
GAATCATAAAATTTAGGTGG+AGG - chr7.2:83660809-83660828 None:intergenic 30.0%
TTAGTCAAGCTAATGGAATA+TGG + chr7.2:83661099-83661118 MS.gene057452:CDS 30.0%
AAGCAAGTTTAATGCTAACC+AGG - chr7.2:83660893-83660912 None:intergenic 35.0%
TCATAAAATTTAGGTGGAGG+AGG - chr7.2:83660806-83660825 None:intergenic 35.0%
ACAAAATCTGATGCAGTTCC+TGG + chr7.2:83660872-83660891 MS.gene057452:CDS 40.0%
ACAGATCATAAAGCCCAGAA+AGG - chr7.2:83661209-83661228 None:intergenic 40.0%
TAAAATTTAGGTGGAGGAGG+TGG - chr7.2:83660803-83660822 None:intergenic 40.0%
!! TTACCGTCTGCTTTTAAGTG+TGG + chr7.2:83660994-83661013 MS.gene057452:intron 40.0%
ATTGGGCTCAAAAGCCTTTC+TGG + chr7.2:83661192-83661211 MS.gene057452:CDS 45.0%
TTGGGCTCAAAAGCCTTTCT+GGG + chr7.2:83661193-83661212 MS.gene057452:CDS 45.0%
! GCTTTTGAGCCCAATTGCTT+GGG - chr7.2:83661187-83661206 None:intergenic 45.0%
!! ATAGCTCTTTTTGGAGGAGG+TGG - chr7.2:83660773-83660792 None:intergenic 45.0%
AATTTAGGTGGAGGAGGTGG+TGG - chr7.2:83660800-83660819 None:intergenic 50.0%
ATTTAGGTGGAGGAGGTGGT+GGG - chr7.2:83660799-83660818 None:intergenic 50.0%
GGGCCACACTTAAAAGCAGA+CGG - chr7.2:83661000-83661019 None:intergenic 50.0%
TTGAGCCCAATTGCTTGGGT+GGG - chr7.2:83661183-83661202 None:intergenic 50.0%
TTTGAGCCCAATTGCTTGGG+TGG - chr7.2:83661184-83661203 None:intergenic 50.0%
! GGCTTTTGAGCCCAATTGCT+TGG - chr7.2:83661188-83661207 None:intergenic 50.0%
!! GGCATAGCTCTTTTTGGAGG+AGG - chr7.2:83660776-83660795 None:intergenic 50.0%
CTTGCCCCACCCAAGCAATT+GGG + chr7.2:83661175-83661194 MS.gene057452:CDS 55.0%
TGAGCCCAATTGCTTGGGTG+GGG - chr7.2:83661182-83661201 None:intergenic 55.0%
TTTAGGTGGAGGAGGTGGTG+GGG - chr7.2:83660798-83660817 None:intergenic 55.0%
!! CTTTTTGGAGGAGGTGGTGG+AGG - chr7.2:83660767-83660786 None:intergenic 55.0%
!! GCTCTTTTTGGAGGAGGTGG+TGG - chr7.2:83660770-83660789 None:intergenic 55.0%
!! GGGGGCATAGCTCTTTTTGG+AGG - chr7.2:83660779-83660798 None:intergenic 55.0%
!! GGTGGGGGCATAGCTCTTTT+TGG - chr7.2:83660782-83660801 None:intergenic 55.0%
ATTGCTTGGGTGGGGCAAGG+AGG - chr7.2:83661174-83661193 None:intergenic 60.0%
CCAATTGCTTGGGTGGGGCA+AGG - chr7.2:83661177-83661196 None:intergenic 60.0%
CCTTGCCCCACCCAAGCAAT+TGG + chr7.2:83661174-83661193 MS.gene057452:CDS 60.0%
! TTAGGTGGAGGAGGTGGTGG+GGG - chr7.2:83660797-83660816 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 83660761 83661230 83660761 ID=MS.gene057452
chr7.2 mRNA 83660761 83661230 83660761 ID=MS.gene057452.t1;Parent=MS.gene057452
chr7.2 exon 83660761 83660893 83660761 ID=MS.gene057452.t1.exon1;Parent=MS.gene057452.t1
chr7.2 CDS 83660761 83660893 83660761 ID=cds.MS.gene057452.t1;Parent=MS.gene057452.t1
chr7.2 exon 83661094 83661230 83661094 ID=MS.gene057452.t1.exon2;Parent=MS.gene057452.t1
chr7.2 CDS 83661094 83661230 83661094 ID=cds.MS.gene057452.t1;Parent=MS.gene057452.t1
Gene Sequence

>MS.gene057452

ATGCCTCCACCACCTCCTCCAAAAAGAGCTATGCCCCCACCACCTCCTCCACCTAAATTTTATGATTCAACTGAAGTTAAAGTACAAGACAAGAGCAATAGTTTACGGAAGACAAAATCTGATGCAGTTCCTGGTTAGCATTAAACTTGCTTTTACTGAAATGAACAACTTTAATATTGTTTTAGATGTGTCATTATATGTTCTCTCTATAATGTGTAGTATGTATGAAACTTTTACCGTCTGCTTTTAAGTGTGGCCCTTTAATTGACTTTGTAATGCTTTCTTTATTGTGCATCTTTTAGATTTGTTTGTAACTTTAGGTATTTATTTCAGATACTTTAGTCAAGCTAATGGAATATGGTGACGACGACGATGACGATGATGATCTAGACGATTCAAATGAGAAAATGCCTCCTTGCCCCACCCAAGCAATTGGGCTCAAAAGCCTTTCTGGGCTTTATGATCTGTAG

Protein sequence

>MS.gene057452.t1

MPPPPPPKRAMPPPPPPPKFYDSTEVKVQDKSNSLRKTKSDAVPDTLVKLMEYGDDDDDDDDLDDSNEKMPPCPTQAIGLKSLSGLYDL