AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07788

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07788.t1 MTR_8g017280 98.370 368 6 0 1 368 1 368 0.0 748
MS.gene07788.t1 MTR_8g017280 98.258 287 5 0 1 287 1 287 0.0 586
MS.gene07788.t1 MTR_2g099980 57.951 371 150 3 1 368 1 368 9.81e-149 427
MS.gene07788.t1 MTR_8g066650 31.879 298 160 9 32 325 76 334 1.82e-28 115
MS.gene07788.t1 MTR_4g050930 28.782 271 154 8 33 301 26 259 1.17e-27 112
MS.gene07788.t1 MTR_4g050930 28.782 271 154 8 33 301 26 259 3.01e-27 112
MS.gene07788.t1 MTR_3g023270 29.259 270 140 8 35 301 11 232 1.99e-26 108
MS.gene07788.t1 MTR_5g087940 27.815 302 171 7 34 334 24 279 9.63e-25 104
MS.gene07788.t1 MTR_7g021630 36.548 197 101 7 27 216 8 187 1.02e-24 103
MS.gene07788.t1 MTR_7g021630 36.548 197 101 7 27 216 8 187 1.03e-24 103
MS.gene07788.t1 MTR_7g084080 27.143 280 162 8 27 301 8 250 7.32e-24 100
MS.gene07788.t1 MTR_4g045680 29.412 306 179 8 24 327 4 274 8.80e-24 101
MS.gene07788.t1 MTR_1g038760 27.434 339 186 11 34 368 13 295 1.40e-23 100
MS.gene07788.t1 MTR_1g038760 27.434 339 186 11 34 368 13 295 1.40e-23 100
MS.gene07788.t1 MTR_3g023330 27.574 272 152 8 34 301 13 243 8.72e-23 98.6
MS.gene07788.t1 MTR_3g463830 29.964 277 145 10 34 301 61 297 4.70e-22 96.3
MS.gene07788.t1 MTR_5g060570 25.455 275 162 7 35 306 16 250 6.93e-22 94.0
MS.gene07788.t1 MTR_5g066560 28.881 277 151 10 35 304 8 245 1.94e-21 94.7
MS.gene07788.t1 MTR_6g088700 29.242 277 151 10 35 306 13 249 4.16e-21 94.0
MS.gene07788.t1 MTR_3g052940 28.731 268 157 6 34 280 26 280 5.45e-21 94.4
MS.gene07788.t1 MTR_3g052940 28.731 268 157 6 34 280 26 280 6.28e-21 94.4
MS.gene07788.t1 MTR_3g052940 28.731 268 157 6 34 280 26 280 7.43e-21 94.0
MS.gene07788.t1 MTR_3g019200 28.808 302 164 14 35 327 7 266 8.82e-21 92.8
MS.gene07788.t1 MTR_7g080440 27.881 269 155 7 35 302 14 244 1.01e-20 92.4
MS.gene07788.t1 MTR_3g052990 26.962 293 178 6 34 301 26 307 1.85e-20 92.8
MS.gene07788.t1 MTR_3g036320 31.731 208 127 5 38 238 22 221 2.77e-20 91.3
MS.gene07788.t1 MTR_3g019240 27.508 309 175 9 34 334 24 291 3.39e-20 91.3
MS.gene07788.t1 MTR_4g023710 32.812 256 113 10 35 280 143 349 7.57e-20 91.3
MS.gene07788.t1 MTR_8g468910 29.348 276 174 7 11 280 27 287 1.80e-19 89.4
MS.gene07788.t1 MTR_6g025160 28.044 271 151 7 34 301 99 328 2.64e-19 88.6
MS.gene07788.t1 MTR_4g092980 28.620 297 170 11 37 327 15 275 3.73e-19 89.0
MS.gene07788.t1 MTR_4g051480 31.746 189 119 4 34 216 23 207 4.17e-19 89.4
MS.gene07788.t1 MTR_6g018600 28.676 272 148 8 34 301 50 279 4.88e-19 88.2
MS.gene07788.t1 MTR_4g092300 31.746 189 119 4 34 216 23 207 5.00e-19 89.4
MS.gene07788.t1 MTR_3g023470 25.902 305 190 10 35 328 14 293 9.55e-19 87.0
MS.gene07788.t1 MTR_4g024780 31.250 192 115 6 33 216 143 325 1.21e-18 87.4
MS.gene07788.t1 MTR_4g024680 29.412 289 146 12 35 316 153 390 1.60e-18 87.8
MS.gene07788.t1 MTR_4g024680 29.474 190 93 8 35 216 521 677 2.13e-12 68.9
MS.gene07788.t1 MTR_4g026550 31.907 257 115 9 35 280 146 353 1.92e-18 87.0
MS.gene07788.t1 MTR_3g014590 30.769 273 141 12 34 301 9 238 3.30e-18 85.5
MS.gene07788.t1 MTR_3g099930 29.545 220 97 7 36 255 17 178 3.60e-18 81.6
MS.gene07788.t1 MTR_4g047950 28.283 297 167 9 34 324 11 267 4.11e-18 85.5
MS.gene07788.t1 MTR_1g094760 29.787 235 108 8 34 255 24 214 4.26e-18 86.3
MS.gene07788.t1 MTR_3g019220 27.273 319 181 14 18 327 8 284 4.75e-18 86.3
MS.gene07788.t1 MTR_1g067190 33.813 139 85 2 34 172 13 144 5.18e-18 84.3
MS.gene07788.t1 MTR_4g023670 30.435 276 133 9 34 300 120 345 5.71e-18 85.5
MS.gene07788.t1 MTR_4g092430 29.717 212 113 6 35 243 42 220 6.29e-18 85.5
MS.gene07788.t1 MTR_3g463820 26.909 275 159 7 34 301 11 250 6.95e-18 83.2
MS.gene07788.t1 MTR_3g014550 32.579 221 111 8 34 252 9 193 7.72e-18 84.3
MS.gene07788.t1 MTR_4g092430 29.717 212 113 6 35 243 42 220 7.72e-18 85.1
MS.gene07788.t1 MTR_3g036330 36.441 118 66 4 32 143 7 121 1.16e-17 83.2
MS.gene07788.t1 MTR_7g017280 32.161 199 109 6 32 216 144 330 1.31e-17 84.3
MS.gene07788.t1 MTR_4g045740 55.072 69 31 0 37 105 21 89 1.37e-17 80.9
MS.gene07788.t1 MTR_3g105060 29.902 204 119 8 36 232 14 200 1.48e-17 83.6
MS.gene07788.t1 MTR_3g099900 29.577 213 134 5 32 236 14 218 1.64e-17 83.6
MS.gene07788.t1 MTR_6g092430 26.872 227 137 4 36 262 10 207 1.75e-17 83.6
MS.gene07788.t1 MTR_3g007790 25.954 262 163 6 35 296 24 254 2.86e-17 82.8
MS.gene07788.t1 MTR_4g092440 35.833 120 71 3 34 150 30 146 3.09e-17 83.2
MS.gene07788.t1 MTR_6g025090 38.053 113 68 2 34 145 50 161 3.19e-17 80.9
MS.gene07788.t1 MTR_3g109982 29.450 309 165 12 34 302 11 306 3.19e-17 83.6
MS.gene07788.t1 MTR_4g092440 35.833 120 71 3 34 150 30 146 3.41e-17 83.2
MS.gene07788.t1 MTR_3g005980 27.778 252 135 9 35 280 16 226 3.45e-17 82.4
MS.gene07788.t1 MTR_4g050940 28.421 190 120 5 36 216 67 249 3.81e-17 83.2
MS.gene07788.t1 MTR_6g018660 27.305 282 144 9 36 301 20 256 3.98e-17 82.4
MS.gene07788.t1 MTR_3g110010 29.073 313 169 12 30 302 7 306 4.04e-17 83.6
MS.gene07788.t1 MTR_3g036240 33.553 152 90 5 34 183 13 155 5.39e-17 82.0
MS.gene07788.t1 MTR_6g009020 32.692 208 119 7 23 212 3 207 5.44e-17 82.0
MS.gene07788.t1 MTR_3g007830 25.954 262 163 6 35 296 10 240 6.40e-17 81.6
MS.gene07788.t1 MTR_4g014260 33.571 140 84 3 34 172 24 155 1.40e-16 80.9
MS.gene07788.t1 MTR_6g088705 36.449 107 64 2 35 139 18 122 1.50e-16 78.6
MS.gene07788.t1 MTR_3g006580 33.824 136 81 3 38 172 35 162 1.51e-16 80.5
MS.gene07788.t1 MTR_5g060750 30.045 223 116 6 35 255 17 201 1.75e-16 80.5
MS.gene07788.t1 MTR_2g090845 27.491 291 164 8 35 295 13 286 1.94e-16 80.1
MS.gene07788.t1 MTR_4g014260 33.571 140 84 3 34 172 24 155 2.08e-16 80.9
MS.gene07788.t1 MTR_7g094030 32.738 168 102 5 30 194 22 181 2.37e-16 80.5
MS.gene07788.t1 MTR_5g044800 30.732 205 117 6 35 228 29 219 3.55e-16 79.7
MS.gene07788.t1 MTR_5g044860 30.882 204 116 6 36 228 30 219 3.59e-16 79.7
MS.gene07788.t1 MTR_4g092360 32.847 137 86 2 11 145 25 157 3.62e-16 79.7
MS.gene07788.t1 MTR_5g460900 30.045 223 117 7 35 255 17 202 4.85e-16 79.0
MS.gene07788.t1 MTR_3g008050 25.670 261 163 6 35 295 10 239 4.89e-16 79.3
MS.gene07788.t1 MTR_3g023490 28.374 289 138 13 36 316 70 297 5.92e-16 79.3
MS.gene07788.t1 MTR_4g051640 37.273 110 61 2 8 116 9 111 1.66e-15 73.9
MS.gene07788.t1 MTR_3g023440 26.132 287 159 9 35 313 2 243 2.47e-15 77.0
MS.gene07788.t1 MTR_6g025270 26.667 270 156 8 34 301 32 261 2.70e-15 77.0
MS.gene07788.t1 MTR_3g005990 32.258 124 83 1 31 154 12 134 2.92e-15 77.0
MS.gene07788.t1 MTR_7g056447 33.775 151 87 4 35 181 8 149 3.19e-15 75.9
MS.gene07788.t1 MTR_3g023630 32.168 143 92 3 32 172 4 143 8.53e-15 75.5
MS.gene07788.t1 MTR_4g104300 28.421 190 120 6 33 216 110 289 1.43e-14 75.1
MS.gene07788.t1 MTR_1g077420 24.528 265 165 10 35 283 3 248 2.21e-14 72.8
MS.gene07788.t1 MTR_1g041245 29.798 198 124 4 36 230 11 196 2.79e-14 73.9
MS.gene07788.t1 MTR_3g087260 27.476 313 147 12 35 302 11 288 3.18e-14 73.9
MS.gene07788.t1 MTR_1g038710 33.028 218 119 8 36 234 19 228 3.91e-14 73.9
MS.gene07788.t1 MTR_7g056430 31.288 163 104 3 35 195 8 164 4.39e-14 74.3
MS.gene07788.t1 MTR_7g075820 29.148 223 131 8 35 246 5 211 4.43e-14 73.2
MS.gene07788.t1 MTR_7g056420 29.448 163 107 2 35 195 8 164 4.97e-14 73.9
MS.gene07788.t1 MTR_7g033945 30.357 112 78 0 36 147 11 122 6.85e-14 69.7
MS.gene07788.t1 MTR_3g027130 45.333 75 41 0 31 105 98 172 9.82e-14 70.9
MS.gene07788.t1 MTR_4g090940 26.754 228 137 7 3 216 131 342 1.07e-13 72.8
MS.gene07788.t1 MTR_7g114710 31.884 138 88 2 36 172 13 145 1.25e-13 71.6
MS.gene07788.t1 MTR_7g056433 33.333 141 87 4 35 172 11 147 1.84e-13 71.2
MS.gene07788.t1 MTR_3g040700 29.452 146 97 2 30 173 37 178 2.23e-13 71.2
MS.gene07788.t1 MTR_0438s0020 43.662 71 40 0 35 105 67 137 2.61e-13 70.9
MS.gene07788.t1 MTR_3g107160 29.108 213 132 6 35 232 9 217 2.81e-13 70.9
MS.gene07788.t1 MTR_8g085430 29.586 169 105 4 36 195 11 174 2.93e-13 70.5
MS.gene07788.t1 MTR_4g056070 30.986 213 110 6 29 205 3 214 3.34e-13 70.9
MS.gene07788.t1 MTR_4g055580 31.325 249 122 10 26 227 91 337 3.48e-13 71.2
MS.gene07788.t1 MTR_3g036250 30.464 151 93 4 36 181 5 148 3.81e-13 70.5
MS.gene07788.t1 MTR_2g101950 24.242 297 179 8 34 299 36 317 4.55e-13 70.1
MS.gene07788.t1 MTR_6g018560 26.812 276 148 11 34 301 32 261 4.67e-13 70.1
MS.gene07788.t1 MTR_2g101950 24.242 297 179 8 34 299 78 359 4.73e-13 70.1
MS.gene07788.t1 MTR_2g082850 32.500 200 106 8 30 214 16 201 6.31e-13 69.7
MS.gene07788.t1 MTR_2g101950 24.415 299 176 9 34 299 77 358 6.36e-13 70.1
MS.gene07788.t1 MTR_2g101950 24.415 299 176 9 34 299 78 359 6.73e-13 70.1
MS.gene07788.t1 MTR_4g055990 30.841 214 111 7 36 212 9 222 7.11e-13 70.1
MS.gene07788.t1 MTR_4g055990 30.698 215 112 7 35 212 8 222 7.67e-13 70.1
MS.gene07788.t1 MTR_6g018570 26.812 276 148 11 34 301 32 261 9.16e-13 69.7
MS.gene07788.t1 MTR_7g033915 32.075 106 72 0 36 141 11 116 9.55e-13 68.9
MS.gene07788.t1 MTR_2g082800 33.133 166 95 5 35 194 26 181 9.96e-13 69.3
MS.gene07788.t1 MTR_4g051510 35.000 100 65 0 35 134 34 133 1.25e-12 68.6
MS.gene07788.t1 MTR_7g032380 29.193 161 107 4 36 194 11 166 1.69e-12 68.2
MS.gene07788.t1 MTR_1g021825 29.381 194 91 6 29 199 10 180 1.76e-12 68.6
MS.gene07788.t1 MTR_7g034110 31.818 110 72 1 36 145 11 117 2.33e-12 67.8
MS.gene07788.t1 MTR_1g021825 29.381 194 91 6 29 199 81 251 2.69e-12 68.2
MS.gene07788.t1 MTR_3g108150 29.091 165 110 3 30 194 7 164 2.95e-12 67.8
MS.gene07788.t1 MTR_1g013560 28.311 219 106 7 14 208 4 195 3.13e-12 67.4
MS.gene07788.t1 MTR_4g134150 28.667 150 94 4 23 172 3 139 3.33e-12 65.9
MS.gene07788.t1 MTR_1g013580 28.205 234 134 6 14 229 4 221 3.57e-12 67.8
MS.gene07788.t1 MTR_7g098510 30.247 162 104 5 36 194 18 173 3.83e-12 67.4
MS.gene07788.t1 MTR_2g091145 28.846 208 112 5 14 203 6 195 3.90e-12 67.8
MS.gene07788.t1 MTR_1g013560 28.311 219 106 7 14 208 4 195 4.26e-12 67.4
MS.gene07788.t1 MTR_8g465380 27.064 218 130 5 36 252 12 201 4.50e-12 67.0
MS.gene07788.t1 MTR_4g024810 29.502 261 144 9 41 300 130 351 4.61e-12 67.4
MS.gene07788.t1 MTR_7g033940 29.927 137 91 2 18 149 71 207 4.62e-12 65.5
MS.gene07788.t1 MTR_3g108560 40.278 72 43 0 35 106 19 90 4.87e-12 66.6
MS.gene07788.t1 MTR_1g095750 39.286 84 50 1 36 118 46 129 4.93e-12 63.5
MS.gene07788.t1 MTR_1g011410 30.000 220 116 8 36 232 315 519 5.25e-12 67.8
MS.gene07788.t1 MTR_3g108560 40.278 72 43 0 35 106 19 90 6.50e-12 66.6
MS.gene07788.t1 MTR_8g445780 28.671 143 99 2 32 172 9 150 8.73e-12 66.2
MS.gene07788.t1 MTR_7g021550 31.606 193 82 10 35 216 14 167 8.84e-12 65.9
MS.gene07788.t1 MTR_4g080590 28.000 175 101 6 35 198 22 182 1.04e-11 65.9
MS.gene07788.t1 MTR_7g115270 25.333 150 109 1 35 181 11 160 1.86e-11 65.5
MS.gene07788.t1 MTR_4g056030 30.797 276 151 13 34 283 7 268 1.86e-11 65.5
MS.gene07788.t1 MTR_1g107385 28.652 178 109 4 35 195 31 207 2.06e-11 65.5
MS.gene07788.t1 MTR_4g080590 28.000 175 101 6 35 198 22 182 2.07e-11 65.5
MS.gene07788.t1 MTR_1g115250 32.877 146 83 4 35 173 31 168 2.52e-11 65.1
MS.gene07788.t1 MTR_2g007450 29.412 153 91 5 36 172 16 167 3.04e-11 64.7
MS.gene07788.t1 MTR_0543s0010 28.916 166 104 4 35 198 85 238 3.12e-11 64.7
MS.gene07788.t1 MTR_1g115245 30.168 179 97 5 31 194 21 186 3.16e-11 63.9
MS.gene07788.t1 MTR_0492s0020 28.916 166 104 4 35 198 85 238 3.26e-11 64.7
MS.gene07788.t1 MTR_3g019180 40.278 72 43 0 33 104 22 93 3.54e-11 64.3
MS.gene07788.t1 MTR_4g051460 46.809 47 25 0 35 81 4 50 3.75e-11 58.9
MS.gene07788.t1 MTR_4g092320 46.809 47 25 0 35 81 4 50 3.75e-11 58.9
MS.gene07788.t1 MTR_1g011400 29.379 177 110 3 33 199 4 175 4.34e-11 64.3
MS.gene07788.t1 MTR_8g095590 29.255 188 117 7 28 209 9 186 4.38e-11 64.3
MS.gene07788.t1 MTR_1880s0010 39.726 73 44 0 33 105 13 85 4.53e-11 62.8
MS.gene07788.t1 MTR_8g465650 27.570 214 137 7 36 236 12 220 4.72e-11 63.9
MS.gene07788.t1 MTR_1g011430 28.902 173 103 4 36 199 7 168 5.57e-11 63.9
MS.gene07788.t1 MTR_4g094735 36.471 85 49 1 8 92 53 132 6.11e-11 61.6
MS.gene07788.t1 MTR_1g090900 35.227 176 102 6 34 205 9 176 7.03e-11 61.6
MS.gene07788.t1 MTR_7g025080 28.188 149 95 5 34 172 4 150 7.07e-11 63.2
MS.gene07788.t1 MTR_2g047858 29.885 174 99 5 36 194 115 280 7.75e-11 63.9
MS.gene07788.t1 MTR_1g048340 25.157 159 115 3 31 186 11 168 7.91e-11 62.8
MS.gene07788.t1 MTR_7g033990 31.731 104 71 0 36 139 11 114 8.32e-11 63.5
MS.gene07788.t1 MTR_4g045900 28.033 239 134 10 15 228 4 229 9.28e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07788.t1 AT1G78840 26.721 247 161 7 41 286 11 238 2.14e-14 74.7
MS.gene07788.t1 AT1G78840 26.721 247 161 7 41 286 11 238 2.14e-14 74.7
MS.gene07788.t1 AT5G53840 34.641 153 84 6 33 180 15 156 2.25e-14 74.3
MS.gene07788.t1 AT1G51370 26.367 311 186 13 29 312 11 305 1.08e-13 72.0
MS.gene07788.t1 AT1G51370 29.237 236 139 9 13 241 1 215 1.38e-13 72.0
MS.gene07788.t1 AT5G22720 30.612 147 95 3 29 172 16 158 1.52e-13 72.0
MS.gene07788.t1 AT1G51370 26.045 311 187 12 29 312 11 305 1.62e-13 72.0
MS.gene07788.t1 AT1G69630 28.313 166 113 4 31 194 13 174 5.51e-13 70.5
MS.gene07788.t1 AT5G02930 33.125 160 92 6 36 191 28 176 5.64e-13 70.5
MS.gene07788.t1 AT5G02920 34.247 146 82 5 35 178 27 160 7.42e-13 68.6
MS.gene07788.t1 AT4G03220 29.070 258 138 12 36 278 24 251 4.25e-12 67.8
MS.gene07788.t1 AT5G56370 30.864 162 106 3 36 196 2 158 2.38e-11 64.7
MS.gene07788.t1 AT1G16930 29.762 168 102 5 34 195 13 170 2.66e-11 65.1
MS.gene07788.t1 AT1G16930 29.762 168 102 5 34 195 13 170 2.66e-11 65.1
MS.gene07788.t1 AT1G16930 29.762 168 102 5 34 195 13 170 2.66e-11 65.1
MS.gene07788.t1 AT5G53635 30.534 262 129 13 38 280 2 229 3.10e-11 64.7
MS.gene07788.t1 AT5G56370 30.864 162 106 3 36 196 2 158 3.63e-11 64.7
MS.gene07788.t1 AT5G56370 30.864 162 106 3 36 196 2 158 3.63e-11 64.7
MS.gene07788.t1 AT4G13985 29.108 213 118 6 36 232 19 214 4.52e-11 64.3
MS.gene07788.t1 AT5G56420 28.333 180 90 5 33 194 3 161 6.13e-11 63.9
MS.gene07788.t1 AT5G56420 28.333 180 90 5 33 194 3 161 6.13e-11 63.9
MS.gene07788.t1 AT5G56420 28.333 180 90 5 33 194 3 161 6.13e-11 63.9
MS.gene07788.t1 AT1G66300 27.746 173 107 4 6 172 1 161 7.37e-11 63.9
MS.gene07788.t1 AT4G00160 27.108 166 116 2 35 198 15 177 9.00e-11 63.5

Find 69 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGTTTAGTCTTCCTTCTT+TGG 0.196025 8.1:+80522305 MS.gene07788:CDS
CGAAAAGGTGGAGGTATATT+TGG 0.219974 8.1:+80521569 MS.gene07788:CDS
TTCTTATTTACTATCGCTTT+GGG 0.227303 8.1:+80521701 MS.gene07788:CDS
TAGCTTTGCCCTATGTTATA+CGG 0.250080 8.1:+80522059 MS.gene07788:CDS
TGGTGGACAGCCAGAGGTTT+TGG 0.297601 8.1:-80521815 None:intergenic
AGGACTCAAATCTGACTTAA+AGG 0.311937 8.1:-80522148 None:intergenic
AACATAGGGCAAAGCTATTT+AGG 0.315127 8.1:-80522053 None:intergenic
AAGACAAAATATGATGAATA+AGG 0.325111 8.1:-80521507 None:intergenic
AAAACTAGTTTACAGGAATC+TGG 0.348610 8.1:+80521382 MS.gene07788:CDS
ACAAACGTCTCTTCTCCTTT+TGG 0.350443 8.1:+80521907 MS.gene07788:CDS
GTTCTTATTTACTATCGCTT+TGG 0.358076 8.1:+80521700 MS.gene07788:CDS
TCATGAATTACACGAACCTT+TGG 0.385218 8.1:+80522347 MS.gene07788:CDS
AATGCCGAAAACTAGTTTAC+AGG 0.387247 8.1:+80521375 None:intergenic
TGGTTTGATCAATATGTTAC+GGG 0.407463 8.1:+80522407 MS.gene07788:CDS
AATCTCCGGGCCAAAACCTC+TGG 0.418965 8.1:+80521805 MS.gene07788:CDS
TTTCAAGAAGTTTGTGTTGT+GGG 0.420527 8.1:+80521645 MS.gene07788:CDS
TTTAGTCTTCCTTCTTTGGA+TGG 0.422418 8.1:+80522309 MS.gene07788:CDS
AGTTCGGCTAAACTTGCAAA+TGG 0.436775 8.1:-80522000 None:intergenic
ATATGATGAATAAGGCAATC+TGG 0.453620 8.1:-80521499 None:intergenic
AGAGGCAACGGAATTTCAAT+TGG 0.454657 8.1:-80521835 None:intergenic
TATGTTACGGGGACATCATA+AGG 0.455100 8.1:+80522419 MS.gene07788:CDS
GAACTTGTGTTCTATCGAAA+AGG 0.461513 8.1:+80521554 MS.gene07788:CDS
GCATGTCACCTAGCCTGTCC+TGG 0.463290 8.1:-80521477 None:intergenic
TCCAATGATTTGCAACCAAA+AGG 0.472560 8.1:-80521922 None:intergenic
TAAGTCAGATTTGAGTCCTA+AGG 0.473916 8.1:+80522152 MS.gene07788:CDS
ATGGTTTGATCAATATGTTA+CGG 0.476561 8.1:+80522406 MS.gene07788:CDS
AAAGGAGAAGAGACGTTTGT+AGG 0.478600 8.1:-80521904 None:intergenic
AAATTGACACAATTAGCAAA+TGG 0.484411 8.1:-80522099 None:intergenic
GCAGATATCACAAAATCCTT+AGG 0.486149 8.1:-80522168 None:intergenic
TTATACGGGAATGGAGCGTC+TGG 0.494898 8.1:+80522074 MS.gene07788:CDS
GGCAACGGAATTTCAATTGG+TGG 0.499782 8.1:-80521832 None:intergenic
TGCCCTATGTTATACGGGAA+TGG 0.502035 8.1:+80522065 MS.gene07788:CDS
AAAAGAGAGGAGACTTTACA+TGG 0.503943 8.1:+80522387 MS.gene07788:CDS
AGCTTTGCCCTATGTTATAC+GGG 0.507164 8.1:+80522060 MS.gene07788:CDS
GAGAAGAGACGTTTGTAGGT+TGG 0.512178 8.1:-80521900 None:intergenic
CAGCAAATCATTCACTCGGT+TGG 0.512294 8.1:+80521618 MS.gene07788:CDS
AAGAAATCAGAATTAATCTC+CGG 0.513913 8.1:+80521791 MS.gene07788:CDS
TTGTGTTGCGACAGGACACC+AGG 0.527358 8.1:+80521459 MS.gene07788:CDS
ATTGAATATGCAGCATTACA+TGG 0.527512 8.1:+80521763 MS.gene07788:CDS
ATGATCAATCCATCCAAAGA+AGG 0.536851 8.1:-80522318 None:intergenic
TTGCGACAGGACACCAGGAC+AGG 0.540944 8.1:+80521464 MS.gene07788:CDS
CTCAAAGAAGGCACAAGGTG+TGG 0.541752 8.1:-80522286 None:intergenic
GCTCCATTCCCGTATAACAT+AGG 0.543154 8.1:-80522068 None:intergenic
CTCCATTCCCGTATAACATA+GGG 0.545398 8.1:-80522067 None:intergenic
TTTGCTGTTGAAGTACAGAC+AGG 0.545777 8.1:-80521602 None:intergenic
TTCAATTGGTGGACAGCCAG+AGG 0.557133 8.1:-80521821 None:intergenic
TGCAAATGGATTTGAAAAGT+CGG 0.561253 8.1:-80521986 None:intergenic
AGGAAGACTAAACTCAAAGA+AGG 0.562750 8.1:-80522298 None:intergenic
CTATACCGCAACTGTACAGA+AGG 0.567862 8.1:-80522460 None:intergenic
ATTTCAAGAAGTTTGTGTTG+TGG 0.581606 8.1:+80521644 MS.gene07788:CDS
AGACTCAAGTTACTGAGTTG+CGG 0.587346 8.1:-80522192 None:intergenic
AGAAATCAGAATTAATCTCC+GGG 0.587391 8.1:+80521792 MS.gene07788:CDS
ACAGGACACCAGGACAGGCT+AGG 0.588272 8.1:+80521469 MS.gene07788:CDS
TTTGAAAAGTCGGCTGCGGT+AGG 0.591591 8.1:-80521976 None:intergenic
CTTGTGTTCTATCGAAAAGG+TGG 0.601646 8.1:+80521557 MS.gene07788:CDS
GTGTTCTATCGAAAAGGTGG+AGG 0.603379 8.1:+80521560 MS.gene07788:CDS
ACTATCCTTCTGTACAGTTG+CGG 0.608906 8.1:+80522455 MS.gene07788:CDS
GTGTATAATTGTGTTGCGAC+AGG 0.610973 8.1:+80521451 MS.gene07788:CDS
CTACGATCTTACATCTAAAG+CGG 0.616468 8.1:-80522224 None:intergenic
TGGATTTGAAAAGTCGGCTG+CGG 0.623356 8.1:-80521980 None:intergenic
AATCTGGTTCTGTTATGAAG+AGG 0.633572 8.1:+80521398 MS.gene07788:CDS
CTAAACTCAAAGAAGGCACA+AGG 0.634438 8.1:-80522291 None:intergenic
TCAACAGCAAATCATTCACT+CGG 0.634747 8.1:+80521614 MS.gene07788:CDS
TCTTATTTACTATCGCTTTG+GGG 0.648748 8.1:+80521702 MS.gene07788:CDS
ATCTCATCGAAATAAAAGAG+AGG 0.652681 8.1:+80522374 MS.gene07788:CDS
TAACTGAAATTGAGAAGCTG+TGG 0.665853 8.1:-80522255 None:intergenic
CTTTGGGGTAGATTATGCGA+CGG 0.678356 8.1:+80521717 MS.gene07788:CDS
TTGCTGTTGAAGTACAGACA+GGG 0.681711 8.1:-80521601 None:intergenic
GGTTTGATCAATATGTTACG+GGG 0.698782 8.1:+80522408 MS.gene07788:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGACAAAATATGATGAATA+AGG - chr8.1:80521510-80521529 None:intergenic 20.0%
!!! TCATCATATTTTGTCTTTTA+TGG + chr8.1:80521513-80521532 MS.gene07788:CDS 20.0%
! AAATTGACACAATTAGCAAA+TGG - chr8.1:80522102-80522121 None:intergenic 25.0%
! AAGAAATCAGAATTAATCTC+CGG + chr8.1:80521791-80521810 MS.gene07788:CDS 25.0%
! ATGGTTTGATCAATATGTTA+CGG + chr8.1:80522406-80522425 MS.gene07788:CDS 25.0%
! TGTTGTAAAACCAAAAAGTT+CGG - chr8.1:80522019-80522038 None:intergenic 25.0%
!! TTCTTATTTACTATCGCTTT+GGG + chr8.1:80521701-80521720 MS.gene07788:CDS 25.0%
AGAAATCAGAATTAATCTCC+GGG + chr8.1:80521792-80521811 MS.gene07788:CDS 30.0%
ATATGATGAATAAGGCAATC+TGG - chr8.1:80521502-80521521 None:intergenic 30.0%
ATCTCATCGAAATAAAAGAG+AGG + chr8.1:80522374-80522393 MS.gene07788:CDS 30.0%
ATTGAATATGCAGCATTACA+TGG + chr8.1:80521763-80521782 MS.gene07788:CDS 30.0%
ATTTCAAGAAGTTTGTGTTG+TGG + chr8.1:80521644-80521663 MS.gene07788:CDS 30.0%
TGGTTTGATCAATATGTTAC+GGG + chr8.1:80522407-80522426 MS.gene07788:CDS 30.0%
TTTCAAGAAGTTTGTGTTGT+GGG + chr8.1:80521645-80521664 MS.gene07788:CDS 30.0%
! AAAACTAGTTTACAGGAATC+TGG + chr8.1:80521382-80521401 MS.gene07788:CDS 30.0%
! GGATCAGTATCTTTTGAATA+AGG + chr8.1:80521738-80521757 MS.gene07788:CDS 30.0%
! GTTCTTATTTACTATCGCTT+TGG + chr8.1:80521700-80521719 MS.gene07788:CDS 30.0%
! TCTTATTTACTATCGCTTTG+GGG + chr8.1:80521702-80521721 MS.gene07788:CDS 30.0%
! TGCAAATGGATTTGAAAAGT+CGG - chr8.1:80521989-80522008 None:intergenic 30.0%
! TTTCGATGAGATTTTTCCAA+AGG - chr8.1:80522366-80522385 None:intergenic 30.0%
!! TTTTAACTCGAGTCTTTTCA+AGG - chr8.1:80521875-80521894 None:intergenic 30.0%
!!! GATTCCTGTAAACTAGTTTT+CGG - chr8.1:80521382-80521401 None:intergenic 30.0%
AAAAGAGAGGAGACTTTACA+TGG + chr8.1:80522387-80522406 MS.gene07788:CDS 35.0%
AACATAGGGCAAAGCTATTT+AGG - chr8.1:80522056-80522075 None:intergenic 35.0%
AGGAAGACTAAACTCAAAGA+AGG - chr8.1:80522301-80522320 None:intergenic 35.0%
AGGACTCAAATCTGACTTAA+AGG - chr8.1:80522151-80522170 None:intergenic 35.0%
ATGATCAATCCATCCAAAGA+AGG - chr8.1:80522321-80522340 None:intergenic 35.0%
CTACGATCTTACATCTAAAG+CGG - chr8.1:80522227-80522246 None:intergenic 35.0%
GAAACATTGAAAAATGCTCG+AGG - chr8.1:80521957-80521976 None:intergenic 35.0%
GAACTTGTGTTCTATCGAAA+AGG + chr8.1:80521554-80521573 MS.gene07788:CDS 35.0%
GCAGATATCACAAAATCCTT+AGG - chr8.1:80522171-80522190 None:intergenic 35.0%
GGTTTGATCAATATGTTACG+GGG + chr8.1:80522408-80522427 MS.gene07788:CDS 35.0%
TAACTGAAATTGAGAAGCTG+TGG - chr8.1:80522258-80522277 None:intergenic 35.0%
TAAGTCAGATTTGAGTCCTA+AGG + chr8.1:80522152-80522171 MS.gene07788:CDS 35.0%
TCAACAGCAAATCATTCACT+CGG + chr8.1:80521614-80521633 MS.gene07788:CDS 35.0%
TCATGAATTACACGAACCTT+TGG + chr8.1:80522347-80522366 MS.gene07788:CDS 35.0%
! TAGCTTTGCCCTATGTTATA+CGG + chr8.1:80522059-80522078 MS.gene07788:CDS 35.0%
! TCCAATGATTTGCAACCAAA+AGG - chr8.1:80521925-80521944 None:intergenic 35.0%
! TGAGTTTAGTCTTCCTTCTT+TGG + chr8.1:80522305-80522324 MS.gene07788:CDS 35.0%
! TTTAGTCTTCCTTCTTTGGA+TGG + chr8.1:80522309-80522328 MS.gene07788:CDS 35.0%
!! AATCTGGTTCTGTTATGAAG+AGG + chr8.1:80521398-80521417 MS.gene07788:CDS 35.0%
!! TCCTTTTGGTTGCAAATCAT+TGG + chr8.1:80521921-80521940 MS.gene07788:CDS 35.0%
AAAGGAGAAGAGACGTTTGT+AGG - chr8.1:80521907-80521926 None:intergenic 40.0%
ACTATCCTTCTGTACAGTTG+CGG + chr8.1:80522455-80522474 MS.gene07788:CDS 40.0%
AGACTCAAGTTACTGAGTTG+CGG - chr8.1:80522195-80522214 None:intergenic 40.0%
AGAGGCAACGGAATTTCAAT+TGG - chr8.1:80521838-80521857 None:intergenic 40.0%
AGCTTTGCCCTATGTTATAC+GGG + chr8.1:80522060-80522079 MS.gene07788:CDS 40.0%
AGTTCGGCTAAACTTGCAAA+TGG - chr8.1:80522003-80522022 None:intergenic 40.0%
CTCCATTCCCGTATAACATA+GGG - chr8.1:80522070-80522089 None:intergenic 40.0%
CTTGTGTTCTATCGAAAAGG+TGG + chr8.1:80521557-80521576 MS.gene07788:CDS 40.0%
GTGTATAATTGTGTTGCGAC+AGG + chr8.1:80521451-80521470 MS.gene07788:CDS 40.0%
TATGTTACGGGGACATCATA+AGG + chr8.1:80522419-80522438 MS.gene07788:CDS 40.0%
TGGCAAGTAAAAAGAGGCAA+CGG - chr8.1:80521850-80521869 None:intergenic 40.0%
! AAGGACTGGCAAGTAAAAAG+AGG - chr8.1:80521856-80521875 None:intergenic 40.0%
! ACAAACGTCTCTTCTCCTTT+TGG + chr8.1:80521907-80521926 MS.gene07788:CDS 40.0%
! CGAAAAGGTGGAGGTATATT+TGG + chr8.1:80521569-80521588 MS.gene07788:CDS 40.0%
! CTAAACTCAAAGAAGGCACA+AGG - chr8.1:80522294-80522313 None:intergenic 40.0%
! GCAAGTTTAGCCGAACTTTT+TGG + chr8.1:80522006-80522025 MS.gene07788:CDS 40.0%
! TTGCTGTTGAAGTACAGACA+GGG - chr8.1:80521604-80521623 None:intergenic 40.0%
!! TTTGCTGTTGAAGTACAGAC+AGG - chr8.1:80521605-80521624 None:intergenic 40.0%
CAGCAAATCATTCACTCGGT+TGG + chr8.1:80521618-80521637 MS.gene07788:CDS 45.0%
CTATACCGCAACTGTACAGA+AGG - chr8.1:80522463-80522482 None:intergenic 45.0%
GAGAAGAGACGTTTGTAGGT+TGG - chr8.1:80521903-80521922 None:intergenic 45.0%
GCTCCATTCCCGTATAACAT+AGG - chr8.1:80522071-80522090 None:intergenic 45.0%
GGCAACGGAATTTCAATTGG+TGG - chr8.1:80521835-80521854 None:intergenic 45.0%
GTGTTCTATCGAAAAGGTGG+AGG + chr8.1:80521560-80521579 MS.gene07788:CDS 45.0%
TGCCCTATGTTATACGGGAA+TGG + chr8.1:80522065-80522084 MS.gene07788:CDS 45.0%
TGGATTTGAAAAGTCGGCTG+CGG - chr8.1:80521983-80522002 None:intergenic 45.0%
!! ACTCGAGTCTTTTCAAGGAC+TGG - chr8.1:80521870-80521889 None:intergenic 45.0%
!! CTTTGGGGTAGATTATGCGA+CGG + chr8.1:80521717-80521736 MS.gene07788:CDS 45.0%
TTTGAAAAGTCGGCTGCGGT+AGG - chr8.1:80521979-80521998 None:intergenic 50.0%
! CTCAAAGAAGGCACAAGGTG+TGG - chr8.1:80522289-80522308 None:intergenic 50.0%
! TTATACGGGAATGGAGCGTC+TGG + chr8.1:80522074-80522093 MS.gene07788:CDS 50.0%
! TTCAATTGGTGGACAGCCAG+AGG - chr8.1:80521824-80521843 None:intergenic 50.0%
AATCTCCGGGCCAAAACCTC+TGG + chr8.1:80521805-80521824 MS.gene07788:CDS 55.0%
TTGTGTTGCGACAGGACACC+AGG + chr8.1:80521459-80521478 MS.gene07788:CDS 55.0%
!! TGGTGGACAGCCAGAGGTTT+TGG - chr8.1:80521818-80521837 None:intergenic 55.0%
ACAGGACACCAGGACAGGCT+AGG + chr8.1:80521469-80521488 MS.gene07788:CDS 60.0%
GCATGTCACCTAGCCTGTCC+TGG - chr8.1:80521480-80521499 None:intergenic 60.0%
TTGCGACAGGACACCAGGAC+AGG + chr8.1:80521464-80521483 MS.gene07788:CDS 60.0%
!! GACAGCCAGAGGTTTTGGCC+CGG - chr8.1:80521813-80521832 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 80521376 80522482 80521376 ID=MS.gene07788
chr8.1 mRNA 80521376 80522482 80521376 ID=MS.gene07788.t1;Parent=MS.gene07788
chr8.1 exon 80521376 80522482 80521376 ID=MS.gene07788.t1.exon1;Parent=MS.gene07788.t1
chr8.1 CDS 80521376 80522482 80521376 ID=cds.MS.gene07788.t1;Parent=MS.gene07788.t1
Gene Sequence

>MS.gene07788

ATGCCGAAAACTAGTTTACAGGAATCTGGTTCTGTTATGAAGAGGTCTAAAAAGACAACTTTGATTTCTCAACATGTGTATAATTGTGTTGCGACAGGACACCAGGACAGGCTAGGTGACATGCCAGATTGCCTTATTCATCATATTTTGTCTTTTATGGAGACAAGAGATGCAATTCGAACTTGTGTTCTATCGAAAAGGTGGAGGTATATTTGGACATCAATTCCCTGTCTGTACTTCAACAGCAAATCATTCACTCGGTTGGTTGATTTCAAGAAGTTTGTGTTGTGGGTTCTATCTCATCGCGATAATTCTCATGTCAAAGTTCTTATTTACTATCGCTTTGGGGTAGATTATGCGACGGATCAGTATCTTTTGAATAAGGTTATTGAATATGCAGCATTACATGGTGTCGAAGAAATCAGAATTAATCTCCGGGCCAAAACCTCTGGCTGTCCACCAATTGAAATTCCGTTGCCTCTTTTTACTTGCCAGTCCTTGAAAAGACTCGAGTTAAAAGATTGCCAACCTACAAACGTCTCTTCTCCTTTTGGTTGCAAATCATTGGACATTTTGCACCTCGAGCATTTTTCAATGTTTCCTACCGCAGCCGACTTTTCAAATCCATTTGCAAGTTTAGCCGAACTTTTTGGTTTTACAACATTGACAGCTTTGCACCTAAATAGCTTTGCCCTATGTTATACGGGAATGGAGCGTCTGGATCCATTTGCTAATTGTGTCAATTTGAAGATTTTGCAATTAAGTGAAATGTCCTTTAAGTCAGATTTGAGTCCTAAGGATTTTGTGATATCTGCTCCGCAACTCAGTAACTTGAGTCTAATTTGCAACCGCTTTAGATGTAAGATCGTAGTTACTTCACCACAGCTTCTCAATTTCAGTTACTTGTATTCCACACCTTGTGCCTTCTTTGAGTTTAGTCTTCCTTCTTTGGATGGATTGATCATTGATGTTCATGAATTACACGAACCTTTGGAAAAATCTCATCGAAATAAAAGAGAGGAGACTTTACATGGTTTGATCAATATGTTACGGGGACATCATAAGGCTGAAGCTGTTAAACTATCCTTCTGTACAGTTGCGGTATAG
Protein sequence

>MS.gene07788.t1

MPKTSLQESGSVMKRSKKTTLISQHVYNCVATGHQDRLGDMPDCLIHHILSFMETRDAIRTCVLSKRWRYIWTSIPCLYFNSKSFTRLVDFKKFVLWVLSHRDNSHVKVLIYYRFGVDYATDQYLLNKVIEYAALHGVEEIRINLRAKTSGCPPIEIPLPLFTCQSLKRLELKDCQPTNVSSPFGCKSLDILHLEHFSMFPTAADFSNPFASLAELFGFTTLTALHLNSFALCYTGMERLDPFANCVNLKILQLSEMSFKSDLSPKDFVISAPQLSNLSLICNRFRCKIVVTSPQLLNFSYLYSTPCAFFEFSLPSLDGLIIDVHELHEPLEKSHRNKREETLHGLINMLRGHHKAEAVKLSFCTVAV