AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07809

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 242 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATACCAAGAATTGGAATT+TGG 0.117963 8.3:+72411966 MS.gene07809:CDS
CTCAAACAGTTGCGCCTTTC+CGG 0.135949 8.3:+72413600 MS.gene07809:CDS
CAGCATAATGCTATTCTTAA+AGG 0.148536 8.3:-72414321 None:intergenic
TCCACTAGCTTTACAAGTTT+TGG 0.161774 8.3:+72412486 MS.gene07809:CDS
CTTAAATTTCATAATTTCTT+TGG 0.169206 8.3:-72415560 None:intergenic
GTGACATGAACAACCTTATT+TGG 0.184676 8.3:+72413859 MS.gene07809:CDS
CAAGCAAGACCATCCTTTAA+AGG 0.198651 8.3:+72412417 MS.gene07809:CDS
CTCGAATTGTTCTTGTTTGA+TGG 0.206509 8.3:-72415632 None:intergenic
TTTAAGCATCAAAGAGTTTC+AGG 0.215940 8.3:+72414217 MS.gene07809:CDS
TGAAACTCTTTGATGCTTAA+AGG 0.216785 8.3:-72414215 None:intergenic
CGACTTTCAGAGTAGTAAAT+TGG 0.225620 8.3:-72414875 None:intergenic
CATAAAACTGGAAAGTCTTA+TGG 0.233590 8.3:+72413698 MS.gene07809:CDS
CTTCTAGATTCAACCAAATA+AGG 0.243370 8.3:-72413872 None:intergenic
TTATGACAATGTATGCTTCT+TGG 0.246096 8.3:+72412051 MS.gene07809:CDS
AGATGCTGCTTGATGAATTA+AGG 0.248714 8.3:+72417272 MS.gene07809:CDS
AGTTCATATGAAGGTTTAAT+TGG 0.258411 8.3:+72415061 MS.gene07809:CDS
AAATGCTCGTAACCCTTTAA+AGG 0.270788 8.3:-72412430 None:intergenic
GTCATAATGGGCAAAGTTCT+TGG 0.276900 8.3:-72412035 None:intergenic
ATGCCCCAAATTCCAATTCT+TGG 0.278771 8.3:-72411971 None:intergenic
TGTCCTCAGATTCAATAAAT+AGG 0.280185 8.3:+72413790 MS.gene07809:CDS
ACAAGGTCTTTGAGTTCATT+AGG 0.299422 8.3:-72411899 None:intergenic
ATTCCAGAAAACCAGAATTC+TGG 0.301499 8.3:+72412521 MS.gene07809:CDS
CGTCACCTCCCTGATTTGTC+TGG 0.311899 8.3:+72413567 MS.gene07809:CDS
AATACCAAGAATTGGAATTT+GGG 0.317017 8.3:+72411967 MS.gene07809:CDS
ATAATGTCAGACCGTCAAAA+AGG 0.328152 8.3:-72413998 None:intergenic
TCAGCAGCCATTGAAGTGTT+TGG 0.331185 8.3:-72417058 None:intergenic
AACTCAGATTCCCAGAATTC+TGG 0.334824 8.3:-72412532 None:intergenic
AGAAGACCCTTTGGCAATCA+TGG 0.336129 8.3:+72417156 MS.gene07809:CDS
GCCCAATCTCTTCTTACCCT+TGG 0.344435 8.3:-72413112 None:intergenic
GAATAGCATTATGCTGGAAT+TGG 0.346995 8.3:+72414328 MS.gene07809:CDS
GTGCTAAGAGCGAGTTATAA+TGG 0.348368 8.3:+72412601 MS.gene07809:CDS
ATTTGGGGCATGGGTGGAAT+AGG 0.348819 8.3:+72411983 MS.gene07809:CDS
GCCAATCATGTTTATTGAAA+AGG 0.350994 8.3:-72414282 None:intergenic
ACAGCAACATTGAACATCTT+TGG 0.354048 8.3:+72413265 MS.gene07809:CDS
TATCAACATTTCCAACTCAT+TGG 0.355479 8.3:+72414544 MS.gene07809:CDS
TTTGTTGAATTTAATCTTGT+CGG 0.355553 8.3:-72416834 None:intergenic
ACTGGATAGGTGCATAAAAC+TGG 0.357881 8.3:+72413686 MS.gene07809:CDS
CATTGGGTAAATGTTTCAAT+AGG 0.363000 8.3:-72417315 None:intergenic
GAGATCACTTACTGAACTTC+GGG 0.365731 8.3:+72413935 MS.gene07809:CDS
AAGGCTCTGGAAGAGACTTC+AGG 0.366241 8.3:-72413206 None:intergenic
AGAAGCATACATTGTCATAA+TGG 0.366458 8.3:-72412048 None:intergenic
AATCTTGTCGGAGAAATAGA+AGG 0.367496 8.3:-72416822 None:intergenic
CCCAATCTCTTCTTACCCTT+GGG 0.372517 8.3:-72413111 None:intergenic
AAGCAAGACCATCCTTTAAA+GGG 0.373575 8.3:+72412418 MS.gene07809:CDS
TATTCTACTGTTGTTGGAAA+TGG 0.373809 8.3:-72412770 None:intergenic
CACACTCTTTGATACTTAGA+AGG 0.374123 8.3:-72414844 None:intergenic
TTCACATAATTCTTCACAAC+CGG 0.375474 8.3:-72413619 None:intergenic
GAAGCATACATTGTCATAAT+GGG 0.375608 8.3:-72412047 None:intergenic
CATACCAGACACATCACTTA+TGG 0.376227 8.3:-72419211 None:intergenic
ATGTACCTGAGTATCATTAG+AGG 0.376418 8.3:-72415494 None:intergenic
GATCGGTGTTTGAACTCTCT+TGG 0.377092 8.3:-72414500 None:intergenic
TCCATGTTCAAATTTGTAAT+CGG 0.381932 8.3:-72414701 None:intergenic
CTGAAAACAATACCAAGAAT+TGG 0.382589 8.3:+72411959 MS.gene07809:CDS
TTGATGCTTAAAGGAAGTTC+TGG 0.384731 8.3:-72414206 None:intergenic
TGCTGTCGTGTTCTCTTAGA+TGG 0.388413 8.3:-72414593 None:intergenic
CATTTGTATTCTACTGTTGT+TGG 0.390931 8.3:-72412776 None:intergenic
TTCAACGCCCCAGACAAATC+AGG 0.392235 8.3:-72413576 None:intergenic
GGTCTTGCTTGAAGGCTCTT+AGG 0.394618 8.3:-72412405 None:intergenic
TTCCAGAAAACCAGAATTCT+GGG 0.398442 8.3:+72412522 MS.gene07809:CDS
ATAAAACTGGAAAGTCTTAT+GGG 0.399441 8.3:+72413699 MS.gene07809:CDS
AGAGCTGAGGTTTGTCTGCC+AGG 0.406109 8.3:+72414467 MS.gene07809:CDS
TCTTCTTACCCTTGGGTATG+TGG 0.406946 8.3:-72413104 None:intergenic
GTCACCTCCCTGATTTGTCT+GGG 0.408028 8.3:+72413568 MS.gene07809:CDS
TTGCCAAAGGGTCTTCTTCA+AGG 0.408057 8.3:-72417150 None:intergenic
TTATATTCAGAATAATCAAT+AGG 0.408407 8.3:-72417121 None:intergenic
GTTGGAAATGTTGATAGTTA+TGG 0.413101 8.3:-72414537 None:intergenic
CACACTTGATACTTTGCTAC+TGG 0.414339 8.3:+72413668 MS.gene07809:CDS
TAAAGGATGGTCTTGCTTGA+AGG 0.416442 8.3:-72412413 None:intergenic
TATAATTGAAGCTGTGGGTT+TGG 0.417091 8.3:-72414442 None:intergenic
TCCTCAAGAGAAAGAAACTT+TGG 0.418506 8.3:+72415522 MS.gene07809:CDS
ATCAACATTTCCAACTCATT+GGG 0.420227 8.3:+72414545 MS.gene07809:CDS
CGAGACAGGCATACTCTTAG+TGG 0.421269 8.3:+72412319 MS.gene07809:CDS
TTCTCCAAGAGTCACATCAT+TGG 0.429610 8.3:-72417332 None:intergenic
GGAAGATGGAAGGAGGGAGG+TGG 0.432903 8.3:+72414652 MS.gene07809:CDS
CGACAGCATGGATACTTTGT+TGG 0.433349 8.3:+72414608 MS.gene07809:CDS
AGCTGATTACGAACATATAT+TGG 0.433453 8.3:-72412106 None:intergenic
TCTAACGAACTAATGTTTGC+AGG 0.433851 8.3:-72414065 None:intergenic
ACAAGAATACTAGATGCCTT+TGG 0.441602 8.3:+72412697 MS.gene07809:CDS
ATCTGTAGATTCTTCACAAA+TGG 0.441866 8.3:-72417004 None:intergenic
CAATGTATGCTTCTTGGAAA+AGG 0.442488 8.3:+72412057 MS.gene07809:CDS
TGCTGAGTATGCTGTCATAA+TGG 0.446809 8.3:-72414751 None:intergenic
TTACCCAATGATGTGACTCT+TGG 0.449847 8.3:+72417328 MS.gene07809:CDS
TTTCATTCAAGTCCGATTGT+TGG 0.452920 8.3:-72415030 None:intergenic
AAACTCTTATCTGGTGAACT+TGG 0.453196 8.3:+72417181 MS.gene07809:CDS
AGGTTGGATTTAAGCAAAAC+CGG 0.453445 8.3:+72413810 MS.gene07809:CDS
CTTTAAGAATAGCATTATGC+TGG 0.453732 8.3:+72414322 MS.gene07809:CDS
GATGCTGCTTGATGAATTAA+GGG 0.453744 8.3:+72417273 MS.gene07809:CDS
ATTCAATTTGAATCAAGATC+AGG 0.455575 8.3:+72414956 MS.gene07809:CDS
AATGCGATGTCAATGCTATA+CGG 0.455793 8.3:+72414631 MS.gene07809:CDS
CTCAGATTCAATAAATAGGT+TGG 0.457455 8.3:+72413794 MS.gene07809:CDS
AAAATATTGCACCCCTCAAT+CGG 0.458506 8.3:+72413837 MS.gene07809:CDS
CATGAGAGTTCTGCGATGTC+TGG 0.460792 8.3:+72415589 MS.gene07809:CDS
AAAGGGTTACGAGCATTTCT+CGG 0.461203 8.3:+72412435 MS.gene07809:CDS
TGAACTTGGTCATTCTTCAC+AGG 0.464608 8.3:+72417195 MS.gene07809:CDS
ACCCAAGGGTAAGAAGAGAT+TGG 0.464772 8.3:+72413110 MS.gene07809:CDS
TGACATACCTTGAGTGGAAT+GGG 0.465112 8.3:+72413178 MS.gene07809:CDS
ATTAGAATTAGAGTTGTATG+AGG 0.467109 8.3:+72414931 MS.gene07809:CDS
TCTCCAAGAGTCACATCATT+GGG 0.469580 8.3:-72417331 None:intergenic
AAGTGAAGACTCAGAAAAGT+TGG 0.471926 8.3:+72412081 MS.gene07809:CDS
GTCTTTCAATAAAGCAGCTA+AGG 0.473818 8.3:+72415696 MS.gene07809:CDS
AATCCTTGAAGAAGACCCTT+TGG 0.474745 8.3:+72417147 MS.gene07809:CDS
GAAGATGGAAGGAGGGAGGT+GGG 0.475230 8.3:+72414653 MS.gene07809:CDS
ATTGGAATTTGGGGCATGGG+TGG 0.475360 8.3:+72411977 MS.gene07809:CDS
TGAGATCACTTACTGAACTT+CGG 0.480062 8.3:+72413934 MS.gene07809:CDS
GATGATTTGTGTCGAGTACT+TGG 0.482222 8.3:+72412259 MS.gene07809:CDS
CCCAAGGGTAAGAAGAGATT+GGG 0.482752 8.3:+72413111 MS.gene07809:CDS
GTGATAATGAGTCTACTGTT+AGG 0.483040 8.3:-72412292 None:intergenic
CTAAAATACTTCAGTGTCAA+AGG 0.483161 8.3:+72413741 MS.gene07809:CDS
TGCTCAGCATGAAAAGGCTC+TGG 0.485208 8.3:-72413219 None:intergenic
CAAAGTGGATACTGTTATCA+AGG 0.485926 8.3:-72414255 None:intergenic
TGATGAGGGAGATGATGAGA+AGG 0.488188 8.3:+72414988 MS.gene07809:CDS
TCCAAAGTTTCTTTCTCTTG+AGG 0.489861 8.3:-72415523 None:intergenic
ACTAAATCCAAACACTTCAA+TGG 0.497913 8.3:+72417051 MS.gene07809:CDS
GAGAGGTACTTGATGCTAGC+AGG 0.499293 8.3:-72414134 None:intergenic
CTGACATACCTTGAGTGGAA+TGG 0.500113 8.3:+72413177 MS.gene07809:CDS
ATCATTGCATGAATTAAGAC+TGG 0.501947 8.3:+72414088 MS.gene07809:CDS
GAGCTGAGGTTTGTCTGCCA+GGG 0.502490 8.3:+72414468 MS.gene07809:CDS
TGTCAATGCTATACGGAAGA+TGG 0.502856 8.3:+72414638 MS.gene07809:CDS
ATCAGCTGCTCAGCATGAAA+AGG 0.502924 8.3:-72413225 None:intergenic
ACATCTTTGGTACGGAATGC+AGG 0.504666 8.3:+72413278 MS.gene07809:CDS
CAGGGGAATGATGCAATTGA+AGG 0.506360 8.3:+72412994 MS.gene07809:intron
CTCTGTTATAATTGAAGCTG+TGG 0.508344 8.3:-72414448 None:intergenic
AAAGACATTTGTGATGTACT+TGG 0.510545 8.3:+72412883 MS.gene07809:CDS
GAGAAGGAGAAGGATTCTAC+TGG 0.510794 8.3:+72415652 MS.gene07809:CDS
TTTAGCCTCTAATGATACTC+AGG 0.511737 8.3:+72415489 MS.gene07809:intron
TTACGAGCATTTCTCGGAAA+GGG 0.514011 8.3:+72412441 MS.gene07809:CDS
TTGTTAGTATAAGAATAAGA+TGG 0.514116 8.3:-72417028 None:intergenic
GTAACTGTTTGCATTCACTT+AGG 0.514791 8.3:-72413545 None:intergenic
GCACGACTTACTACAAAAGA+TGG 0.515008 8.3:+72412798 MS.gene07809:CDS
ACCCTCGCTTGACCGCATTA+CGG 0.519545 8.3:+72414355 MS.gene07809:CDS
AGGGTGTAAATCATCATCAC+TGG 0.522062 8.3:-72416802 None:intergenic
AGGCTCTGGAAGAGACTTCA+GGG 0.522506 8.3:-72413205 None:intergenic
TACTCCATAAGTGATGTGTC+TGG 0.524599 8.3:+72419207 MS.gene07809:CDS
CTCAAGCGAGACAATTCAAT+CGG 0.524643 8.3:-72413915 None:intergenic
TCATTATCACAACGCGAGAC+AGG 0.524930 8.3:+72412305 MS.gene07809:CDS
ACAAACTGACATACCTTGAG+TGG 0.525061 8.3:+72413172 MS.gene07809:CDS
TATCAAGGAGGTGCAGTTGT+CGG 0.525267 8.3:-72414240 None:intergenic
AGAATTGGAATTTGGGGCAT+GGG 0.525390 8.3:+72411974 MS.gene07809:CDS
GTTGTTCATGTCACCGATTG+AGG 0.527559 8.3:-72413850 None:intergenic
TTCCGTAATGCGGTCAAGCG+AGG 0.528769 8.3:-72414357 None:intergenic
AACATTGAACATCTTTGGTA+CGG 0.535293 8.3:+72413270 MS.gene07809:CDS
GAGGGAGATGATGAGAAGGA+AGG 0.538301 8.3:+72414992 MS.gene07809:CDS
TTTGCATTCACTTAGGTCGA+TGG 0.538358 8.3:-72413538 None:intergenic
TCTCTTGGAACTCTGCGCCC+TGG 0.538710 8.3:-72414485 None:intergenic
TTGATACTTTGCTACTGGAT+AGG 0.540286 8.3:+72413673 MS.gene07809:CDS
AGTGAAGACTCAGAAAAGTT+GGG 0.542792 8.3:+72412082 MS.gene07809:CDS
GATGATGAGAAGGAAGGTAC+TGG 0.545861 8.3:+72414998 MS.gene07809:CDS
GGAAATGTTGATAGTTATGG+AGG 0.547773 8.3:-72414534 None:intergenic
CAACCTATTTATTGAATCTG+AGG 0.548949 8.3:-72413793 None:intergenic
TGTCGAGTACTTGGTGACCT+TGG 0.549216 8.3:+72412268 MS.gene07809:CDS
TTGTTCATGTCACCGATTGA+GGG 0.551001 8.3:-72413849 None:intergenic
TTTGGTTTCAATGCAACTAG+TGG 0.551362 8.3:+72412715 MS.gene07809:CDS
GCTTGATGAATTAAGGGAGT+TGG 0.553479 8.3:+72417279 MS.gene07809:CDS
TGGATTATCATGGCGACAGA+AGG 0.556204 8.3:+72412621 MS.gene07809:CDS
TCAGTCGGCTACGCTTTCCT+CGG 0.556547 8.3:-72412852 None:intergenic
TTGAATTTGCATGAGAACAA+AGG 0.556606 8.3:+72412553 MS.gene07809:CDS
AAGAATTGGAATTTGGGGCA+TGG 0.557108 8.3:+72411973 MS.gene07809:CDS
GAGAAGGATTCTACTGGAGG+AGG 0.557336 8.3:+72415658 MS.gene07809:CDS
GAAAGGGCAGTTGAATGTGC+AGG 0.558702 8.3:+72412457 MS.gene07809:CDS
GCTCGTAACCCTTTAAAGGA+TGG 0.560792 8.3:-72412426 None:intergenic
TATTGAAAAGGTCTTCAAAG+TGG 0.561977 8.3:-72414270 None:intergenic
TTGCATGAATTAAGACTGGA+TGG 0.562161 8.3:+72414092 MS.gene07809:CDS
TGGCATACAGAACCAACAAT+CGG 0.562843 8.3:+72415018 MS.gene07809:CDS
TTGGTTTCAATGCAACTAGT+GGG 0.563485 8.3:+72412716 MS.gene07809:CDS
CGAGTTATAATGGATTATCA+TGG 0.564248 8.3:+72412611 MS.gene07809:CDS
TCTGACTAATACATTGTGAA+AGG 0.564390 8.3:-72414408 None:intergenic
CTTAAATTCCACATACCCAA+GGG 0.564696 8.3:+72413096 MS.gene07809:CDS
TCAACGCCCCAGACAAATCA+GGG 0.565827 8.3:-72413575 None:intergenic
TCTGTTATAATTGAAGCTGT+GGG 0.566649 8.3:-72414447 None:intergenic
TCCGTAATGCGGTCAAGCGA+GGG 0.568344 8.3:-72414356 None:intergenic
CCCAAAACTTGTAAAGCTAG+TGG 0.570182 8.3:-72412487 None:intergenic
AGCATTATGCTGGAATTGGA+TGG 0.571419 8.3:+72414332 MS.gene07809:CDS
TGGAGGAAGATGTGGTTGAT+CGG 0.571469 8.3:-72414517 None:intergenic
TCTTAAATTCCACATACCCA+AGG 0.572528 8.3:+72413095 MS.gene07809:CDS
TCAAGTCATCGATAAAATTG+TGG 0.573040 8.3:+72411844 MS.gene07809:CDS
TTCTTCTCGAACTATATCAA+AGG 0.573976 8.3:-72412821 None:intergenic
GTTACGAGCATTTCTCGGAA+AGG 0.576759 8.3:+72412440 MS.gene07809:CDS
AGTATGCTGTCATAATGGTA+TGG 0.582142 8.3:-72414746 None:intergenic
GACTTCACACGTTCATCAAG+AGG 0.583105 8.3:+72412176 MS.gene07809:CDS
AGAACAATTCGAGAAGGAGA+AGG 0.583538 8.3:+72415642 MS.gene07809:CDS
TGAGTCTACTGTTAGGTCCA+AGG 0.584415 8.3:-72412285 None:intergenic
GGGGATACCCATTCCACTCA+AGG 0.585578 8.3:-72413185 None:intergenic
CTAACGAACTAATGTTTGCA+GGG 0.585791 8.3:-72414064 None:intergenic
TGTAGATTCTTCACAAATGG+TGG 0.590386 8.3:-72417001 None:intergenic
TGAGTTCATTAGGATACATG+AGG 0.596123 8.3:-72411889 None:intergenic
TTACAACTTATACAAGCAGC+GGG 0.597058 8.3:+72414807 MS.gene07809:CDS
ACCGATTACAAATTTGAACA+TGG 0.597658 8.3:+72414700 MS.gene07809:CDS
AGGTGGGATATAAGTCTAAG+TGG 0.598539 8.3:+72414669 MS.gene07809:CDS
GATAGTTATGGAGGAAGATG+TGG 0.599262 8.3:-72414525 None:intergenic
CAAAGGCTGCAAGAGTCTGA+AGG 0.601671 8.3:+72413758 MS.gene07809:CDS
ATCTTGTCGGAGAAATAGAA+GGG 0.601927 8.3:-72416821 None:intergenic
ATCAGGTGAAGGCTATGATG+AGG 0.604000 8.3:+72414973 MS.gene07809:CDS
TGTCTTTAGCATCCCTCAGT+CGG 0.605943 8.3:-72412867 None:intergenic
AAAAGTTGATGAAATATATG+AGG 0.606212 8.3:+72412342 MS.gene07809:CDS
TTTACTACTCTGAAAGTCGG+AGG 0.606593 8.3:+72414879 MS.gene07809:CDS
AAGCAGAAGCTACTGAAACT+CGG 0.607197 8.3:+72417236 MS.gene07809:CDS
AGCAGCGGGAGAGAACTTGA+TGG 0.607677 8.3:+72414821 MS.gene07809:CDS
TGATGTGACTCTTGGAGAAG+AGG 0.609711 8.3:+72417336 MS.gene07809:CDS
GAATTGTTCTTGTTTGATGG+AGG 0.610137 8.3:-72415629 None:intergenic
TGTTCATGTCACCGATTGAG+GGG 0.610466 8.3:-72413848 None:intergenic
TCAGGTGAAGGCTATGATGA+GGG 0.611440 8.3:+72414974 MS.gene07809:CDS
TGATGTACTTGGAAATAACA+AGG 0.613634 8.3:+72412894 MS.gene07809:CDS
ATACCAAGAATTGGAATTTG+GGG 0.614912 8.3:+72411968 MS.gene07809:CDS
TTACTACTCTGAAAGTCGGA+GGG 0.615979 8.3:+72414880 MS.gene07809:CDS
TCTAAGAGAACACGACAGCA+TGG 0.619055 8.3:+72414596 MS.gene07809:CDS
AAACAAGTCGAAGACAAATG+AGG 0.619269 8.3:+72416854 MS.gene07809:CDS
AATGCTATACGGAAGATGGA+AGG 0.620724 8.3:+72414642 MS.gene07809:CDS
CAAACAAGAACAATTCGAGA+AGG 0.625426 8.3:+72415636 MS.gene07809:CDS
AGGAAAGCGTAGCCGACTGA+GGG 0.626250 8.3:+72412855 MS.gene07809:CDS
CTTCAATTATAACAGAGCTG+AGG 0.627599 8.3:+72414454 MS.gene07809:CDS
ATAATTCTTCACAACCGGAA+AGG 0.629027 8.3:-72413614 None:intergenic
GCTATACGGAAGATGGAAGG+AGG 0.629455 8.3:+72414645 MS.gene07809:CDS
AAGGAGAAGGATTCTACTGG+AGG 0.632082 8.3:+72415655 MS.gene07809:CDS
TGTTCTCATCAACTTTAACA+AGG 0.632895 8.3:-72411916 None:intergenic
ATTACAACTTATACAAGCAG+CGG 0.635481 8.3:+72414806 MS.gene07809:CDS
GAGTTCATTAGGATACATGA+GGG 0.635568 8.3:-72411888 None:intergenic
AAATATATGAGGTCAAAACA+TGG 0.636401 8.3:+72412353 MS.gene07809:CDS
TTTGAATCAAGATCAGGTGA+AGG 0.637513 8.3:+72414962 MS.gene07809:CDS
TGATGAACGTGTGAAGTCCG+TGG 0.644251 8.3:-72412171 None:intergenic
CTTCTAAGTATCAAAGAGTG+TGG 0.646026 8.3:+72414845 MS.gene07809:CDS
GGTGGGATATAAGTCTAAGT+GGG 0.646830 8.3:+72414670 MS.gene07809:CDS
CAATTTACTACTCTGAAAGT+CGG 0.649765 8.3:+72414876 MS.gene07809:CDS
TACGGAAGATGGAAGGAGGG+AGG 0.651783 8.3:+72414649 MS.gene07809:CDS
ACTGGATGGAAGCAGTGTGG+AGG 0.656771 8.3:+72414106 MS.gene07809:CDS
ATTACAACATCTGATGTCCA+CGG 0.657095 8.3:+72412154 MS.gene07809:CDS
CTATACGGAAGATGGAAGGA+GGG 0.658179 8.3:+72414646 MS.gene07809:CDS
TCACCTCCCTGATTTGTCTG+GGG 0.660625 8.3:+72413569 MS.gene07809:CDS
ACAGGAACTTGTGAATCTAG+AGG 0.660839 8.3:+72413515 MS.gene07809:intron
CGAGAAGAATATAATGACCG+AGG 0.662053 8.3:+72412835 MS.gene07809:CDS
GAGGAAAGCGTAGCCGACTG+AGG 0.662165 8.3:+72412854 MS.gene07809:CDS
AGATTCTTCACAAATGGTGG+TGG 0.665267 8.3:-72416998 None:intergenic
AGATGGCATCTTCCGTAATG+CGG 0.668794 8.3:-72414367 None:intergenic
AGGGCAGTTGAATGTGCAGG+AGG 0.669451 8.3:+72412460 MS.gene07809:CDS
ACTAGTTGCATTGAAACCAA+AGG 0.670419 8.3:-72412713 None:intergenic
TTCACACGTTCATCAAGAGG+AGG 0.673704 8.3:+72412179 MS.gene07809:CDS
GGCTCTGGAAGAGACTTCAG+GGG 0.692132 8.3:-72413204 None:intergenic
AGTGGATACTGTTATCAAGG+AGG 0.710096 8.3:-72414252 None:intergenic
AAGACTGGATGGAAGCAGTG+TGG 0.711489 8.3:+72414103 MS.gene07809:CDS
ACTGAACTTCTAGCTCTGAG+AGG 0.714091 8.3:-72414151 None:intergenic
TATATGAGGTCAAAACATGG+AGG 0.733275 8.3:+72412356 MS.gene07809:CDS
ACGCCCCAGACAAATCAGGG+AGG 0.788691 8.3:-72413572 None:intergenic
AGATGTTCAATGTTGCTGTG+CGG 0.790821 8.3:-72413261 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr8.3 gene 72411835 72419245 72411835 ID=MS.gene07809
chr8.3 mRNA 72411835 72419245 72411835 ID=MS.gene07809.t1;Parent=MS.gene07809
chr8.3 exon 72411835 72412915 72411835 ID=MS.gene07809.t1.exon1;Parent=MS.gene07809.t1
chr8.3 CDS 72411835 72412915 72411835 ID=cds.MS.gene07809.t1;Parent=MS.gene07809.t1
chr8.3 exon 72412997 72413299 72412997 ID=MS.gene07809.t1.exon2;Parent=MS.gene07809.t1
chr8.3 CDS 72412997 72413299 72412997 ID=cds.MS.gene07809.t1;Parent=MS.gene07809.t1
chr8.3 exon 72413519 72415082 72413519 ID=MS.gene07809.t1.exon3;Parent=MS.gene07809.t1
chr8.3 CDS 72413519 72415082 72413519 ID=cds.MS.gene07809.t1;Parent=MS.gene07809.t1
chr8.3 exon 72415494 72415717 72415494 ID=MS.gene07809.t1.exon4;Parent=MS.gene07809.t1
chr8.3 CDS 72415494 72415717 72415494 ID=cds.MS.gene07809.t1;Parent=MS.gene07809.t1
chr8.3 exon 72416789 72416875 72416789 ID=MS.gene07809.t1.exon5;Parent=MS.gene07809.t1
chr8.3 CDS 72416789 72416875 72416789 ID=cds.MS.gene07809.t1;Parent=MS.gene07809.t1
chr8.3 exon 72416974 72417357 72416974 ID=MS.gene07809.t1.exon6;Parent=MS.gene07809.t1
chr8.3 CDS 72416974 72417357 72416974 ID=cds.MS.gene07809.t1;Parent=MS.gene07809.t1
chr8.3 exon 72419198 72419245 72419198 ID=MS.gene07809.t1.exon7;Parent=MS.gene07809.t1
chr8.3 CDS 72419198 72419245 72419198 ID=cds.MS.gene07809.t1;Parent=MS.gene07809.t1
Gene Sequence

>MS.gene07809

GGATGATTCTCAAGTCATCGATAAAATTGTGGAAGATGTTTTGCAAAAGTTGTCCCTCATGTATCCTAATGAACTCAAAGACCTTGTTAAAGTTGATGAGAACAGTGAACATATTGAATTATTACTGAAAACAATACCAAGAATTGGAATTTGGGGCATGGGTGGAATAGGAAAGACAACCATAGCAAAACAGATGTTTGCCAAGAACTTTGCCCATTATGACAATGTATGCTTCTTGGAAAAGGTAAGTGAAGACTCAGAAAAGTTGGGACCAATATATGTTCGTAATCAGCTTCTTCGTGAGCTACTGAAGCGAGAAATTACAACATCTGATGTCCACGGACTTCACACGTTCATCAAGAGGAGGCTCTTTCGCAAAAAAGTTTTCATTGTACTTGATGATGTTGATAATGCTACACAGTTAGATGATTTGTGTCGAGTACTTGGTGACCTTGGACCTAACAGTAGACTCATTATCACAACGCGAGACAGGCATACTCTTAGTGGAAAAGTTGATGAAATATATGAGGTCAAAACATGGAGGCTTAAAGACTCTCTAAAGCTTTTTAGCCTAAGAGCCTTCAAGCAAGACCATCCTTTAAAGGGTTACGAGCATTTCTCGGAAAGGGCAGTTGAATGTGCAGGAGGTGTTCCACTAGCTTTACAAGTTTTGGGTTCACATTTTCATTCCAGAAAACCAGAATTCTGGGAATCTGAGTTGAATTTGCATGAGAACAAAGGAGAATCCTTGCCCGATATTCAAAAAGTGCTAAGAGCGAGTTATAATGGATTATCATGGCGACAGAAGGAAATGTTTCTAGACATTGCATTCTTTTTCAAAGGTGAAAACAAAGACATCGTCACAAGAATACTAGATGCCTTTGGTTTCAATGCAACTAGTGGGATAGAAATCCTCGAAGATAAAACTCTTATAACCATTTCCAACAACAGTAGAATACAAATGCACGACTTACTACAAAAGATGGCCTTTGATATAGTTCGAGAAGAATATAATGACCGAGGAAAGCGTAGCCGACTGAGGGATGCTAAAGACATTTGTGATGTACTTGGAAATAACAAGGTATGGAGTATTAAATTTTTCTATATAAAAAATTACTGTTGATTTGTTGTGGTAATAATGTAGTGACTTTCAATTTGTCAGGGGAATGATGCAATTGAAGGAATAATATTTGATTTGTCTCAAAAACTAGATATACATGTTCAAGCTGACACATTCAAATTGATGACTAAATTAAGATTTCTTAAATTCCACATACCCAAGGGTAAGAAGAGATTGGGCACTGTGCATCTTCCTGAAAATATTATGCCATTTTTTGACAAACTGACATACCTTGAGTGGAATGGGTATCCCCTGAAGTCTCTTCCAGAGCCTTTTCATGCTGAGCAGCTGATTAAGATTTCTTTGCCGCACAGCAACATTGAACATCTTTGGTACGGAATGCAGGTGCGTGTGTGTACTTAAACCAATTCATTTGAATTATGGAATTCTTTCTTAGTTTTTTATACAAATGAAAATTAGAAAAATTATTAAATAATGCGAGTCACATGCAGTTGTGTTTTCTTCGATTGCATGGTGTGAAAATGAATGGATGCTTGACAGACCAATTTAAATAAAATATGAGAATATTAATAACCTTTGACGTGTTCTTTTGAATTGGTACAGGAACTTGTGAATCTAGAGGCCATCGACCTAAGTGAATGCAAACAGTTACGTCACCTCCCTGATTTGTCTGGGGCGTTGAAACTCAAACAGTTGCGCCTTTCCGGTTGTGAAGAATTATGTGAACTTCAACCTTCTGCTTTTTCTAAGGACACACTTGATACTTTGCTACTGGATAGGTGCATAAAACTGGAAAGTCTTATGGGTGAGAAGCATTTAACATCTCTAAAATACTTCAGTGTCAAAGGCTGCAAGAGTCTGAAGGAATTTTCATTGTCCTCAGATTCAATAAATAGGTTGGATTTAAGCAAAACCGGAATCAAAATATTGCACCCCTCAATCGGTGACATGAACAACCTTATTTGGTTGAATCTAGAAGATTTAAATCTAACGAATCTACCGATTGAATTGTCTCGCTTGAGATCACTTACTGAACTTCGGGTTTCTAAGTGCAGTGTAGTAACTAAATCCAATTTAGAAGCCCTTTTTGACGGTCTGACATTATTGAGATTACTGCACTTGAAAGACTGTTGTAACTTGATTGAACTCCCTGCAAACATTAGTTCGTTAGAATCATTGCATGAATTAAGACTGGATGGAAGCAGTGTGGAGGAGTTGCCTGCTAGCATCAAGTACCTCTCAGAGCTAGAAGTTCAGTCTTTAGACAATTGCAGTAAGCTTCGTCGTCTGCCAGAACTTCCTTTAAGCATCAAAGAGTTTCAGGCCGACAACTGCACCTCCTTGATAACAGTATCCACTTTGAAGACCTTTTCAATAAACATGATTGGCCAAAAAAAATACATTTCCTTTAAGAATAGCATTATGCTGGAATTGGATGGACCCTCGCTTGACCGCATTACGGAAGATGCCATCTTAACAATGAAAAGTGCTGCCTTTCACAATGTATTAGTCAGAAAGTACAGATTCCAAACCCACAGCTTCAATTATAACAGAGCTGAGGTTTGTCTGCCAGGGCGCAGAGTTCCAAGAGAGTTCAAACACCGATCAACCACATCTTCCTCCATAACTATCAACATTTCCAACTCATTGGGCTTCATTTTTGCAGTTGTTGTTTCTCCATCTAAGAGAACACGACAGCATGGATACTTTGTTGGAATGCGATGTCAATGCTATACGGAAGATGGAAGGAGGGAGGTGGGATATAAGTCTAAGTGGGATCATAAACCGATTACAAATTTGAACATGGATCATGTTTTTGTATGGTATGATCCATACCATTATGACAGCATACTCAGCAGTATTGAAAGAAAGATTTCTTTTAAATTCTACATTACAACTTATACAAGCAGCGGGAGAGAACTTGATGGCCTTCTAAGTATCAAAGAGTGTGGAGTTTGTCCAATTTACTACTCTGAAAGTCGGAGGGTTTTAGGCACAGGGAATTTAGACAAGAAATTAGAATTAGAGTTGTATGAGGAAATTCAATTTGAATCAAGATCAGGTGAAGGCTATGATGAGGGAGATGATGAGAAGGAAGGTACTGGCATACAGAACCAACAATCGGACTTGAATGAAAATTTTCACAGTTCATATGAAGGTTTAATTGGTACGAAACTCTTTGCTCTCTTTCACAATTGTTTTATGATATCTTTCTTATGTTGTTAATCAATCCCTCCGTACAATATAGTTTGAATAGCATTTAGAATATAGTCCGTATTTCTTCCCTTTCATATTCAATTTTAGAGAAACCAGATGAAACTAACAATATTTTATAAAAATTTATATATCTAAACATTATATAGACTGCTTTATTTCATTTTCCTTCATCGTCAAATTTTACAATCGTCTCAGAGAGTAGAGTTGCCTTTAAGTCTATTATTATGCACATACCCACACCCTTATTAATTGCAATTGCATGCTTTCTTTTTCACTGAATTGCAATTGCCTACTTGTAACTTTTAAATCTTTAAGATAGTCGATTTTTTCTTTGTCAACCTCACCATTTCTTTTTCTTTAGCCTCTAATGATACTCAGGTACATGAAAATCCTCAAGAGAAAGAAACTTTGGATGATGATGATAATTCCAAAGAAATTATGAAATTTAAGATTGTTCATGAGAGTTCTGCGATGTCTGGAGACGAAACTGAAACTTCCTCCATCAAACAAGAACAATTCGAGAAGGAGAAGGATTCTACTGGAGGAGGTTCTGATGTAGATTTGTCTTTCAATAAAGCAGCTAAGGCAATACTTGCACCTTTGACCTTTTTATTCCATCTAAATATATCACACTCATCCTCTTTCTCTTTTCAAATAGTGTTTTAGTAATGTTTCTAAGCAGCGGAAAATGAAAGCTCAGCATAAAACCCATTTTATAGAGCCTAATGAAGAACGTACAGTGGCACGCGCGTGCGCGAACACACACACATACAAACACTCACACTACACCCACATACACATATGTTATTTTCCTTACACTCTCCCCTCAAATAAAGAAATTTTGTTTTCGATCATCGTTTTTTTATAACCTTGATCATTGTTGTTTTGTTTTAATCTTTGTAATATATTTTTATGTTGCATTCATCTTTATGAGAGGAACAACTTACTTTGAAAGGGTGTGAAAAAAAAATCTGTTAGATGAGCAATTAGAACAATATTATAGCATGTCCTAAAATTAATACTGTTCATATATTACAAGTATTAAAACCAACATGAACAAATATTACATATACTAAAATCCAAACCAACTGTTTGGACTCAATTTGTTAATGAGTGTTATGTTAATGTCGAGTCAATCAGAAGTATTCCATTTCTATTGGAAGTACCTAAGTTCGATCCATGGTTGGAACTTGGAACAATGTTTCATCATACTTTACTTATTAACAAAATTCATGAACAACATAAGAAAGCAAACAATATCAACAGAATTGAGAGAATAAAATGGATTGCAAGAGACTTTGAAAGTTATTCTCATTAATTCAAATTGAACGGAGGTTACAAAATCAGTTACAAGGAGATAGTGGACACACCAAATATGTTGAGTAACCTCTTTGGCCCATCACAATGGGCCTTTATCCGGATGGTACATACCTCGTTGCCGAATTCCGAATTATTAGAATAGATTTCCTCTGTGAATTAGAGGTATAAGACTAAAATCAATATCAAAACCAAAATTTTTTCTGGGTTAGGGGCGAAAACAATTTTTTATAGCTATAAGAGCAAAAACAGAGATATTTTTGCATGTGAAAAAAAAAAATTGAATTTATTTTTTTATTTCCTTTTAACTTGTCATTTACTTTTCTCACCTTTCAACAGAGACAAAAGTTTTCCAGTGATGATGATTTACACCCTTCTATTTCTCCGACAAGATTAAATTCAACAAACAAGTCGAAGACAAATGAGGTTTTTCTTTGTTCCTTAGCGACATGAAACAACCTTCATTCGTAATGCAAACTACTTTATTCCTTAATAATACTTATGGTTGATTGTTTCATTACCAGGAAACTAAGAATCTTCAAGAGTCACCACCACCATTTGTGAAGAATCTACAGATTCCATCTTATTCTTATACTAACAAACTAAATCCAAACACTTCAATGGCTGCTGAGAGTTCTTCAAGTAAACCTTCGAATGATTCAAAATATTCTCCTATTGATTATTCTGAATATAAGAAAATCCTTGAAGAAGACCCTTTGGCAATCATGGAAAAACTCTTATCTGGTGAACTTGGTCATTCTTCACAGGCTTCTCAATCTACACAACAAGCAGAAGCTACTGAAACTCGGAGTGAGTCAATCAAGATGCTGCTTGATGAATTAAGGGAGTTGGCATTTTCAAGAAACCTATTGAAACATTTACCCAATGATGTGACTCTTGGAGAAGAGGTGAAAGCTTTATTAGTTAAACTCAATTATAGAGCTAATGAGCTTTCTGAGAAACAAAGTTCTGGTATTACTGATTTCACCAAAATCTTTATAGATGCCACACTGAATATTGATGAAGGAAAACTGAGCAATGTTACTCTTCAACATCTTAATGTGGATCATGAAGATTCAATATCCAAGCTTCAAGCATCCAAAGACAAGATAATGAAGTTTGACAAGTCCATCAGTACCGCTGAGGATAAGATCAAGGCCAGGGATGTTGAAATTGAAGATATCAAAGCACAGATTTGTTTGCTCGAGGAGAAAGCACGTAAGGTTCAACAAGAGAAGTCACAGTTAGAGGATGCTTGCTCAAAATGTAAGGAAAAGAGAACTGAAATTGTGGAAGAAGCTAAAAATGTAGCCTCTATGACCATACAAACCCGCGAGAAGATTGATAATGTAAACAAGAAGAAGCGGGAGTTGGATTCAAATTATGACACGCTTGAAGGAAATTATGCCATAATGAGACTGGCACCTCCATTTTAGAATTTGCCTTATTATTATGCTTGTTATATTTCCTTTATTTTTGAACAATTTATAAACCATCAAAATTTAGTTCACTGTATATCACTCTCTCGTACTTAATCTTTAAATTATATAAAAATAATAATAATGATAAAAAAAAAATTCTCAAAAAGTTTCTCCTTTTCTTGCGAATTTCGTCCAGAAAATGCATCCTATATTGAGAACATTTTCAAAGACTTTGTCATGAGTAGAAAAGTGGTTGAGGATCACATGAATGCTTTACAGCAAAGAGAAAAGCTTATGCAGCGAGTGAGAGATGCTAAGAAACAGAAGGAATCAAAAGAGAAAGAAAAGGTGGATGAAAAAATATTAGAGTTCTTGTTGAGCAAAAGAAAAGTACTGAGTTGGAAAAAAACAAAGTTGAGAACATAGAAGATACTGCTAAGTACTCCCTCCGGTCCTAGTTACAAGAGACAATTTACTTTTCAGATACATTAAATAATTTATGTATTTAGTTTAGGTTCTTGACTAGGTACATACATTATTTAATGTATCTAAAAAGTCAACTTTCTCTTGTAAATGGGACCGGAGGGAGTATATGGTAATTGAAAGAAAAAAAGTCGATGTCGAGTATTAAGAATTCTAAATCTTCATATGCTGCTTTTTTGCAGACAACTATGGAAGTATAGAATAGCTGAGAAGTGTTGATAGCATGCATGAAGCTTAAATTCTGATTGAAGGCATGTATTTATTTGTAGACCAATTATGAAACTAAGTGTCATTTTTGTGAAAAGTTATACTACATTAATAATCAAAACCACAATACATATATCTTCATCACAAATTTGAATGCTAATCTTATATCTATTCATCCATGAATATAAAACTTTTTTTATTTTCTTTTTTACGGTACAAATTAGTATGACCCATGAATATAATATTAACTTTTAACATATATGAAATTCATCAATTAGTCTCTAAATTTTTTAAAAAGTCATCATTATAATTCTTAACATCTTAATAACAAAAGACTAAATTAATGAATTTTATATAGTTTGAAGACTACTTGATATATTTATGTAGATTAGGGATTAAATTGGAGAGAAGTTGATAATTTAGGGAGTAGTTTGATGGTTTATTCTAATTAAATTAATCTCTAAGTTCTTAACTATTCTTATAGTTTCACTAATTTTTCATTCTTGTATACTTTAAATTGATATAAAATAGTCTTTATAACCGTTAATTTTTAAATAATTTGCTTTCATATTAACAATGCTAATAATATTTTGTTAAGTTTTTATATATCAACAAAATAAATTTAAATAAACAAAAGATGTTGATCTACTCCATAAGTGATGTGTCTGGTATGAACATTGCATAA
Protein sequence

>MS.gene07809.t1

GUFSSHRUNCGRCFAKVVPHVSUUTQRPCUSUUEQUTYUIITENNTKNWNLGHGWNRKDNHSKTDVCQELCPLUQCMLLGKGKURLRKVGTNICSUSASSUATEARNYNIUCPRTSHVHQEEALSQKSFHCTUUCUUCYTVRUFVSSTWUPWTUQUTHYHNARQAYSUWKSUUNIUGQNMEAURLSKAFUPKSLQARPSFKGLRAFLGKGSUMCRRCSTSFTSFGFTFSFQKTRILGIUVEFAUEQRRILARYSKSAKSELUWIIMATEGNVSRHCILFQRUKQRHRHKNTRCLWFQCNUWDRNPRRUNSYNHFQQQUNTNARLTTKDGLUYSSRRIUUPRKAUPTEGCURHLUCTWKUQGEUCNURNNIUFVSKTRYTCSSUHIQIDDUIKISUIPHTQGUEEIGHCASSUKYYAIFUQTDIPUVEWVSPEVSSRAFSCUAADUDFFAAQQHUTSLVRNAGTCESRGHRPKUMQTVTSPPUFVWGVETQTVAPFRLURIMUTSTFCFFUGHTUYFATGUVHKTGKSYGUEAFNISKILQCQRLQESEGIFIVLRFNKUVGFKQNRNQNIAPLNRUHEQPYLVESRRFKSNESTDUIVSLEITYUTSGFUVQCSNUIQFRSPFURSDIIEITALERLLULDUTPCKHUFVRIIAUIKTGWKQCGGVACUHQVPLRARSSVFRQLQUASSSARTSFKHQRVSGRQLHLLDNSIHFEDLFNKHDWPKKIHFLUEUHYAGIGWTLAUPHYGRCHLNNEKCCLSQCISQKVQIPNPQLQLUQSUGLSARAQSSKRVQTPINHIFLHNYQHFQLIGLHFCSCCFSIUENTTAWILCWNAMSMLYGRWKEGGGIUVUVGSUTDYKFEHGSCFCMVUSIPLUQHTQQYUKKDFFUILHYNLYKQRERTUWPSKYQRVWSLSNLLLUKSEGFRHREFRQEIRIRVVUGNSIUIKIRURLUUGRUUEGRYWHTEPTIGLEUKFSQFIURFNCLUUYSGTUKSSRERNFGUUUUFQRNYEIUDCSUEFCDVWRRNUNFLHQTRTIREGEGFYWRRFUCRFVFQUSSUETKVFQUUUFTPFYFSDKIKFNKQVEDKUGNUESSRVTTTICEESTDSILFLYUQTKSKHFNGCUEFFKUTFEUFKIFSYULFUIUENPURRPFGNHGKTLIWUTWSFFTGFSIYTTSRSYUNSEUVNQDAAUUIKGVGIFKKPIETFTQUCDSWRRDVDLLHKUCVWYEHCI