AlfalfaGEDB | MS.gene07828

M. sativa cultivar XinJiangDaYe / MS.gene07828

KEGG: K15718
GO: GO:0003674 GO:0003824 GO:0005488 GO:0008150 GO:0008152 GO:0016491 GO:0016701 GO:0016702 GO:0043167 GO:0043169 GO:0046872 GO:0051213 GO:0055114
PFAM: PF00305.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07828.t1	XP_003627173.1	98.9	189	2	0	1	189	416	604	5.70E-102	380.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07828.t1	P09918	82.5	189	33	0	1	189	422	610	1.2e-87	323.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07828.t1	G7LI99	98.9	189	2	0	1	189	416	604	4.1e-102	380.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052728	MS.gene07828	0.864157	1.49E-64	-1.69E-46
MS.gene052730	MS.gene07828	0.821338	4.26E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07828.t1	MTR_8g018420	98.942	189	2	0	1	189	416	604	4.77e-131	388
MS.gene07828.t1	MTR_2g099570	81.481	189	35	0	1	189	422	610	1.01e-106	325
MS.gene07828.t1	MTR_8g018570	80.952	189	36	0	1	189	430	618	2.08e-106	324
MS.gene07828.t1	MTR_8g018550	76.720	189	44	0	1	189	428	616	1.12e-102	314
MS.gene07828.t1	MTR_8g020990	76.720	189	44	0	1	189	428	616	4.02e-102	313
MS.gene07828.t1	MTR_2g099560	77.249	189	43	0	1	189	424	612	7.07e-101	310
MS.gene07828.t1	MTR_8g018690	73.545	189	50	0	1	189	425	613	2.85e-99	305
MS.gene07828.t1	MTR_8g018690	73.545	189	50	0	1	189	405	593	3.08e-99	305
MS.gene07828.t1	MTR_8g018520	75.132	189	47	0	1	189	428	616	3.18e-99	305
MS.gene07828.t1	MTR_8g018510	74.737	190	47	1	1	189	428	617	3.22e-96	297
MS.gene07828.t1	MTR_8g018430	71.958	189	53	0	1	189	421	609	5.59e-96	296
MS.gene07828.t1	MTR_8g018735	73.545	189	49	1	1	189	427	614	9.30e-95	288
MS.gene07828.t1	MTR_8g018730	71.429	189	53	1	1	189	146	333	1.18e-92	281
MS.gene07828.t1	MTR_8g018735	73.545	189	49	1	1	189	427	614	1.67e-92	288
MS.gene07828.t1	MTR_8g018450	68.586	191	58	1	1	189	386	576	5.32e-92	285
MS.gene07828.t1	MTR_3g081130	70.000	190	56	1	1	189	346	535	3.93e-91	282
MS.gene07828.t1	MTR_7g113410	69.841	189	57	0	1	189	428	616	4.74e-91	284
MS.gene07828.t1	MTR_3g081130	70.000	190	56	1	1	189	418	607	1.23e-90	283
MS.gene07828.t1	MTR_8g018730	71.429	189	53	1	1	189	396	583	1.43e-90	282
MS.gene07828.t1	MTR_8g018730	71.429	189	53	1	1	189	426	613	2.42e-90	282
MS.gene07828.t1	MTR_5g024020	69.312	189	57	1	1	189	393	580	4.42e-89	278
MS.gene07828.t1	MTR_5g024020	69.312	189	57	1	1	189	429	616	7.44e-89	278
MS.gene07828.t1	MTR_1g083020	69.841	189	57	0	1	189	419	607	1.70e-88	277
MS.gene07828.t1	MTR_1g104650	67.725	189	61	0	1	189	412	600	2.46e-88	276
MS.gene07828.t1	MTR_8g018590	66.316	190	61	1	1	187	381	570	3.42e-85	268
MS.gene07828.t1	MTR_8g018650	64.362	188	65	1	2	189	370	555	3.57e-83	262
MS.gene07828.t1	MTR_8g018620	63.158	190	67	2	1	189	369	556	6.91e-83	261
MS.gene07828.t1	MTR_8g021020	74.342	152	35	1	42	189	28	179	8.26e-77	236
MS.gene07828.t1	MTR_8g011340	53.684	190	84	2	1	189	426	612	3.09e-67	220
MS.gene07828.t1	MTR_6g452500	53.158	190	85	2	1	189	181	367	3.20e-67	216
MS.gene07828.t1	MTR_8g080230	52.821	195	80	4	1	189	475	663	2.48e-59	199
MS.gene07828.t1	MTR_8g020970	52.356	191	87	4	1	189	487	675	6.18e-59	195
MS.gene07828.t1	MTR_8g020970	52.356	191	87	4	1	189	487	675	8.89e-58	194
MS.gene07828.t1	MTR_3g479460	50.515	194	84	4	1	188	462	649	9.79e-55	186
MS.gene07828.t1	MTR_3g079450	50.526	190	90	3	1	188	461	648	1.00e-53	181
MS.gene07828.t1	MTR_3g079450	50.526	190	90	3	1	188	461	648	8.29e-53	181
MS.gene07828.t1	MTR_4g066170	51.053	190	89	4	1	188	464	651	1.92e-52	179
MS.gene07828.t1	MTR_4g088810	47.644	191	90	3	1	189	461	643	7.24e-52	178
MS.gene07828.t1	MTR_3g079420	50.262	191	90	4	1	188	423	611	8.01e-51	175
MS.gene07828.t1	MTR_7g012030	62.105	95	35	1	95	188	87	181	6.62e-36	125
MS.gene07828.t1	MTR_6g046330	54.717	106	36	2	95	188	77	182	5.04e-30	112
MS.gene07828.t1	MTR_8g463810	37.821	156	70	5	2	156	197	326	1.18e-23	96.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07828.t1	AT3G22400	66.138	189	64	0	1	189	442	630	7.96e-89	278
MS.gene07828.t1	AT1G55020	65.789	190	63	2	1	189	420	608	4.12e-83	263
MS.gene07828.t1	AT3G45140	51.282	195	83	4	1	189	313	501	1.63e-59	198
MS.gene07828.t1	AT3G45140	51.282	195	83	4	1	189	455	643	4.57e-59	198
MS.gene07828.t1	AT1G67560	51.813	193	86	4	1	189	475	664	8.72e-58	195
MS.gene07828.t1	AT1G17420	51.309	191	89	4	1	189	479	667	2.48e-57	193
MS.gene07828.t1	AT1G72520	50.785	191	91	3	1	189	485	674	3.14e-56	190

Find 35 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATATCTTCAAAAGTTTATA+AGG	0.223035	8.3:+72207987	MS.gene07828:CDS
TGATGGAACTTTAAAGTCTT+TGG	0.226575	8.3:+72207415	MS.gene07828:CDS
AGCAAGTGCATTTATATTCA+TGG	0.295232	8.3:-72207896	None:intergenic
CTTCAAAAGTTTATAAGGAT+TGG	0.319001	8.3:+72207992	MS.gene07828:CDS
ACACAATAATTCCATATCTA+AGG	0.334272	8.3:+72207333	MS.gene07828:CDS
TTCAAAAGTTTATAAGGATT+GGG	0.366464	8.3:+72207993	MS.gene07828:CDS
GAGTTTATTAGCCTTAGATA+TGG	0.386116	8.3:-72207344	None:intergenic
GCTTCAACACCTTCAATTGC+AGG	0.390029	8.3:-72207503	None:intergenic
GTCTACTTGCCTGCAATTGA+AGG	0.425065	8.3:+72207494	MS.gene07828:CDS
TAAGATCAGCAGGGAGGGCT+TGG	0.426671	8.3:-72208026	None:intergenic
CTGTTCGTTGCTATGACAAA+TGG	0.429152	8.3:-72207817	None:intergenic
GGATGCAATAGTTTGTAAAT+AGG	0.441730	8.3:-72207859	None:intergenic
TACTGTTTGGCTACTTGCCA+AGG	0.479396	8.3:+72207526	MS.gene07828:CDS
TATTGAAAAGACATTCTTGT+GGG	0.498429	8.3:+72207948	MS.gene07828:CDS
CATCCTCAAGGAGATCAATT+CGG	0.504730	8.3:+72207458	MS.gene07828:CDS
AATTGATCTCCTTGAGGATG+TGG	0.505930	8.3:-72207455	None:intergenic
AATGCCATCTGCATTGACTA+AGG	0.506196	8.3:-72207926	None:intergenic
TTCACCAACTTGTCAGCCAC+TGG	0.513084	8.3:+72207576	MS.gene07828:CDS
AAGGATGGTTCTTGAAGCAT+AGG	0.529557	8.3:-72207381	None:intergenic
AAGGTGTTGAAGCTACTGTT+TGG	0.533014	8.3:+72207513	MS.gene07828:CDS
GTTTACTATAACATAAGCCT+TGG	0.534747	8.3:-72207543	None:intergenic
AAAAGACATTCTTGTGGGGA+GGG	0.536208	8.3:+72207953	MS.gene07828:CDS
ATGTGGAAGACTTAGCTCAA+TGG	0.546020	8.3:-72207438	None:intergenic
TCCCTGCTGATCTTATCAAA+AGG	0.550271	8.3:+72208034	MS.gene07828:CDS
TTTACCAGTGGCTGACAAGT+TGG	0.560303	8.3:-72207580	None:intergenic
GAAAAGACATTCTTGTGGGG+AGG	0.560508	8.3:+72207952	MS.gene07828:CDS
TTTGATAAGATCAGCAGGGA+GGG	0.567663	8.3:-72208031	None:intergenic
CTAAGTCTTCCACATCCTCA+AGG	0.600779	8.3:+72207446	MS.gene07828:CDS
TTCATGGTATCGCGATAATG+TGG	0.601143	8.3:-72207880	None:intergenic
TTATTGAAAAGACATTCTTG+TGG	0.603556	8.3:+72207947	MS.gene07828:CDS
TCATCCTTAGTCAATGCAGA+TGG	0.613787	8.3:+72207922	MS.gene07828:CDS
GATATGGAATTATTGTGTCA+TGG	0.613887	8.3:-72207328	None:intergenic
CTTGTGGGGAGGGTATGCTA+TGG	0.623212	8.3:+72207963	MS.gene07828:CDS
ATTGAAAAGACATTCTTGTG+GGG	0.649770	8.3:+72207949	MS.gene07828:CDS
ACACCGAATTGATCTCCTTG+AGG	0.687282	8.3:-72207461	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATATCTTCAAAAGTTTATA+AGG	+	chr8.3:72207987-72208006	MS.gene07828:CDS	15.0%
!!	AATCAATAAATACTAATAGA+TGG	+	chr8.3:72207623-72207642	MS.gene07828:intron	15.0%
!!	TTCAAAAGTTTATAAGGATT+GGG	+	chr8.3:72207993-72208012	MS.gene07828:CDS	20.0%
!	ACACAATAATTCCATATCTA+AGG	+	chr8.3:72207333-72207352	MS.gene07828:CDS	25.0%
!	ATTTAGTGACATAATTAGAG+AGG	+	chr8.3:72207713-72207732	MS.gene07828:intron	25.0%
!	CTTCAAAAGTTTATAAGGAT+TGG	+	chr8.3:72207992-72208011	MS.gene07828:CDS	25.0%
!	TACTCATAGTCAATTTAGAT+AGG	-	chr8.3:72207755-72207774	None:intergenic	25.0%
!	TATTGAAAAGACATTCTTGT+GGG	+	chr8.3:72207948-72207967	MS.gene07828:CDS	25.0%
!	TTATTGAAAAGACATTCTTG+TGG	+	chr8.3:72207947-72207966	MS.gene07828:CDS	25.0%
!!	GTCACTAAATTTTACAACAA+AGG	-	chr8.3:72207703-72207722	None:intergenic	25.0%
!!	TTGACTATGAGTAATTTTGA+TGG	+	chr8.3:72207762-72207781	MS.gene07828:intron	25.0%
	AGCAAGTGCATTTATATTCA+TGG	-	chr8.3:72207899-72207918	None:intergenic	30.0%
	ATTGAAAAGACATTCTTGTG+GGG	+	chr8.3:72207949-72207968	MS.gene07828:CDS	30.0%
	GATATGGAATTATTGTGTCA+TGG	-	chr8.3:72207331-72207350	None:intergenic	30.0%
	GTTTACTATAACATAAGCCT+TGG	-	chr8.3:72207546-72207565	None:intergenic	30.0%
	TACAACAAAGGAATTAGAGA+GGG	-	chr8.3:72207691-72207710	None:intergenic	30.0%
	TTACAACAAAGGAATTAGAG+AGG	-	chr8.3:72207692-72207711	None:intergenic	30.0%
!	ATCCTTTTCTTGAAAAGTGA+TGG	+	chr8.3:72207398-72207417	MS.gene07828:CDS	30.0%
!	GGATGCAATAGTTTGTAAAT+AGG	-	chr8.3:72207862-72207881	None:intergenic	30.0%
!!	ATCAACTATGTTTTTACCAG+TGG	-	chr8.3:72207595-72207614	None:intergenic	30.0%
!!	ATCACTTTTCAAGAAAAGGA+TGG	-	chr8.3:72207399-72207418	None:intergenic	30.0%
!!	GAGTTTATTAGCCTTAGATA+TGG	-	chr8.3:72207347-72207366	None:intergenic	30.0%
!!	TGATGGAACTTTAAAGTCTT+TGG	+	chr8.3:72207415-72207434	MS.gene07828:CDS	30.0%
!!	TTCCATCACTTTTCAAGAAA+AGG	-	chr8.3:72207403-72207422	None:intergenic	30.0%
	GAATTAGAGAGGGAATTCAT+TGG	-	chr8.3:72207681-72207700	None:intergenic	35.0%
!!!	GAGTAATTTTGATGGATTGC+AGG	+	chr8.3:72207770-72207789	MS.gene07828:intron	35.0%
	AAAAGACATTCTTGTGGGGA+GGG	+	chr8.3:72207953-72207972	MS.gene07828:CDS	40.0%
	AAGGATGGTTCTTGAAGCAT+AGG	-	chr8.3:72207384-72207403	None:intergenic	40.0%
	AATTGATCTCCTTGAGGATG+TGG	-	chr8.3:72207458-72207477	None:intergenic	40.0%
	ATGTGGAAGACTTAGCTCAA+TGG	-	chr8.3:72207441-72207460	None:intergenic	40.0%
	CATCCTCAAGGAGATCAATT+CGG	+	chr8.3:72207458-72207477	MS.gene07828:CDS	40.0%
	CTGTTCGTTGCTATGACAAA+TGG	-	chr8.3:72207820-72207839	None:intergenic	40.0%
	TCATCCTTAGTCAATGCAGA+TGG	+	chr8.3:72207922-72207941	MS.gene07828:CDS	40.0%
	TCCCTGCTGATCTTATCAAA+AGG	+	chr8.3:72208034-72208053	MS.gene07828:CDS	40.0%
	TTCATGGTATCGCGATAATG+TGG	-	chr8.3:72207883-72207902	None:intergenic	40.0%
	TTTGATAAGATCAGCAGGGA+GGG	-	chr8.3:72208034-72208053	None:intergenic	40.0%
!	TCTTGAAGCATAGGCTTTTG+TGG	-	chr8.3:72207375-72207394	None:intergenic	40.0%
!	TTTTGATAAGATCAGCAGGG+AGG	-	chr8.3:72208035-72208054	None:intergenic	40.0%
!!	AAGGTGTTGAAGCTACTGTT+TGG	+	chr8.3:72207513-72207532	MS.gene07828:CDS	40.0%
!!	AATGCCATCTGCATTGACTA+AGG	-	chr8.3:72207929-72207948	None:intergenic	40.0%
	ACACCGAATTGATCTCCTTG+AGG	-	chr8.3:72207464-72207483	None:intergenic	45.0%
	CTAAGTCTTCCACATCCTCA+AGG	+	chr8.3:72207446-72207465	MS.gene07828:CDS	45.0%
	GAAAAGACATTCTTGTGGGG+AGG	+	chr8.3:72207952-72207971	MS.gene07828:CDS	45.0%
	GCTTCAACACCTTCAATTGC+AGG	-	chr8.3:72207506-72207525	None:intergenic	45.0%
	GTCTACTTGCCTGCAATTGA+AGG	+	chr8.3:72207494-72207513	MS.gene07828:CDS	45.0%
	TACTGTTTGGCTACTTGCCA+AGG	+	chr8.3:72207526-72207545	MS.gene07828:CDS	45.0%
	TTTACCAGTGGCTGACAAGT+TGG	-	chr8.3:72207583-72207602	None:intergenic	45.0%
	TTCACCAACTTGTCAGCCAC+TGG	+	chr8.3:72207576-72207595	MS.gene07828:CDS	50.0%
	TAAGATCAGCAGGGAGGGCT+TGG	-	chr8.3:72208029-72208048	None:intergenic	55.0%
!	CTTGTGGGGAGGGTATGCTA+TGG	+	chr8.3:72207963-72207982	MS.gene07828:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	72207293	72208055	72207293	ID=MS.gene07828
chr8.3	mRNA	72207293	72208055	72207293	ID=MS.gene07828.t1;Parent=MS.gene07828
chr8.3	exon	72207293	72207597	72207293	ID=MS.gene07828.t1.exon1;Parent=MS.gene07828.t1
chr8.3	CDS	72207293	72207597	72207293	ID=cds.MS.gene07828.t1;Parent=MS.gene07828.t1
chr8.3	exon	72207792	72208055	72207792	ID=MS.gene07828.t1.exon2;Parent=MS.gene07828.t1
chr8.3	CDS	72207792	72208055	72207792	ID=cds.MS.gene07828.t1;Parent=MS.gene07828.t1

Gene Sequence

>MS.gene07828

GCTATACAAAACAAGAAATTGTTCTTGCTAGAACACCATGACACAATAATTCCATATCTAAGGCTAATAAACTCAACTTCCACAAAAGCCTATGCTTCAAGAACCATCCTTTTCTTGAAAAGTGATGGAACTTTAAAGTCTTTGGCCATTGAGCTAAGTCTTCCACATCCTCAAGGAGATCAATTCGGTGTTGTTAGTAATGTCTACTTGCCTGCAATTGAAGGTGTTGAAGCTACTGTTTGGCTACTTGCCAAGGCTTATGTTATAGTAAACGACTCGTGCTTTCACCAACTTGTCAGCCACTGGTAAAAACATAGTTGATGATATTTGAATCAATAAATACTAATAGATGGAATAAAATTAGCAATGAAAATTAGCGACTAAACCAATGAATTCCCTCTCTAATTCCTTTGTTGTAAAATTTAGTGACATAATTAGAGAGGAATTACACACGTTTTTCCTATCTAAATTGACTATGAGTAATTTTGATGGATTGCAGGTTAAATACTCATGCAGTTGTTGAACCATTTGTCATAGCAACGAACAGACAACTTAGTGTGCTTCATCCTATTTACAAACTATTGCATCCACATTATCGCGATACCATGAATATAAATGCACTTGCTAGATCATCCTTAGTCAATGCAGATGGCATTATTGAAAAGACATTCTTGTGGGGAGGGTATGCTATGGAAATATCTTCAAAAGTTTATAAGGATTGGGTTTTTACAGACCAAGCCCTCCCTGCTGATCTTATCAAAAG

Protein sequence

>MS.gene07828.t1

AIQNKKLFLLEHHDTIIPYLRLINSTSTKAYASRTILFLKSDGTLKSLAIELSLPHPQGDQFGVVSNVYLPAIEGVEATVWLLAKAYVIVNDSCFHQLVSHWLNTHAVVEPFVIATNRQLSVLHPIYKLLHPHYRDTMNINALARSSLVNADGIIEKTFLWGGYAMEISSKVYKDWVFTDQALPADLIK