Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07867.t1 | AES65228.2 | 93 | 100 | 7 | 0 | 3 | 102 | 128 | 227 | 1.10E-43 | 186 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07867.t1 | Q0JBP5 | 75.0 | 32 | 8 | 0 | 103 | 134 | 56 | 87 | 6.2e-06 | 52.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07867.t1 | A0A072V727 | 93.0 | 100 | 7 | 0 | 3 | 102 | 64 | 163 | 8.2e-44 | 186.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05265 | MS.gene07867 | 0.832275 | 1.04E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07867.t1 | MTR_2g036890 | 92.079 | 101 | 8 | 0 | 2 | 102 | 63 | 163 | 7.17e-59 | 192 |
| MS.gene07867.t1 | MTR_2g036890 | 92.079 | 101 | 8 | 0 | 2 | 102 | 63 | 163 | 1.05e-58 | 192 |
| MS.gene07867.t1 | MTR_2g036890 | 92.079 | 101 | 8 | 0 | 2 | 102 | 127 | 227 | 1.70e-58 | 192 |
| MS.gene07867.t1 | MTR_2g012650 | 90.816 | 98 | 9 | 0 | 5 | 102 | 66 | 163 | 1.04e-56 | 186 |
| MS.gene07867.t1 | MTR_2g012650 | 90.816 | 98 | 9 | 0 | 5 | 102 | 66 | 163 | 1.32e-56 | 186 |
| MS.gene07867.t1 | MTR_2g012650 | 90.816 | 98 | 9 | 0 | 5 | 102 | 66 | 163 | 1.55e-56 | 186 |
| MS.gene07867.t1 | MTR_2g012650 | 90.816 | 98 | 9 | 0 | 5 | 102 | 155 | 252 | 3.29e-56 | 186 |
| MS.gene07867.t1 | MTR_2g012650 | 90.816 | 98 | 9 | 0 | 5 | 102 | 154 | 251 | 3.78e-56 | 186 |
| MS.gene07867.t1 | MTR_4g415340 | 72.449 | 98 | 10 | 1 | 5 | 102 | 66 | 146 | 4.01e-40 | 135 |
| MS.gene07867.t1 | MTR_1g015830 | 93.750 | 32 | 2 | 0 | 103 | 134 | 37 | 68 | 3.25e-13 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07867.t1 | AT1G17690 | 39.744 | 78 | 43 | 2 | 28 | 101 | 149 | 226 | 4.40e-11 | 59.7 |
Find 0 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATATATTTTCATATTAAGT+AGG | - | 57448:16040-16059 | MS.gene07867:CDS | 10.0% |
| !! | ATAAGTTATTCCAAAATTAT+TGG | - | 57448:16566-16585 | MS.gene07867:CDS | 15.0% |
| !! | CATTCTAAATAGATTTAATT+TGG | - | 57448:16612-16631 | MS.gene07867:CDS | 15.0% |
| !!! | AATTATGAAAGTATTTAACA+TGG | + | 57448:15593-15612 | None:intergenic | 15.0% |
| !!! | TTGTATAAAAAAAGTATGAA+CGG | + | 57448:15553-15572 | None:intergenic | 15.0% |
| !! | ATATCAATTTGGTTATGTTT+GGG | - | 57448:16459-16478 | MS.gene07867:intron | 20.0% |
| !! | ATATCTCAGAAAATGTATAA+TGG | - | 57448:16374-16393 | MS.gene07867:intron | 20.0% |
| !! | TTTCTAATTGGAATAAAGAT+TGG | + | 57448:15513-15532 | None:intergenic | 20.0% |
| !!! | GTTATTGATTGATATCAATT+TGG | - | 57448:16448-16467 | MS.gene07867:intron | 20.0% |
| !!! | TTTGATATTAGTTTATCATC+TGG | - | 57448:16659-16678 | MS.gene07867:CDS | 20.0% |
| ! | AATGAATGAATACATCCTAT+TGG | + | 57448:16067-16086 | None:intergenic | 25.0% |
| ! | ACAAAAACCAATTCATTTCT+AGG | + | 57448:16640-16659 | None:intergenic | 25.0% |
| ! | ATTCATTCATTTCCTTATAG+AGG | - | 57448:16076-16095 | MS.gene07867:CDS | 25.0% |
| ! | CAAAAACCAATTCATTTCTA+GGG | + | 57448:16639-16658 | None:intergenic | 25.0% |
| ! | GATATCAATTTGGTTATGTT+TGG | - | 57448:16458-16477 | MS.gene07867:intron | 25.0% |
| !! | AAAAAAAGTATGAACGGAAA+TGG | + | 57448:15547-15566 | None:intergenic | 25.0% |
| !! | AATCAAAACTTTTCTATCCA+AGG | + | 57448:15463-15482 | None:intergenic | 25.0% |
| !! | ATATATTTTCTTTCACACCT+TGG | - | 57448:15443-15462 | MS.gene07867:intron | 25.0% |
| !! | GAGAAACAAACCAATAATTT+TGG | + | 57448:16579-16598 | None:intergenic | 25.0% |
| !! | GCAAGATTTTCATTTCTAAT+TGG | + | 57448:15525-15544 | None:intergenic | 25.0% |
| !! | TTAATGTTGTTGTCTTTTTG+CGG | - | 57448:16282-16301 | MS.gene07867:intron | 25.0% |
| !!! | AGTTTTGAACTTACAAAATG+AGG | + | 57448:15984-16003 | None:intergenic | 25.0% |
| !!! | TATAGTGTTTGAGTTTGTTT+TGG | + | 57448:16024-16043 | None:intergenic | 25.0% |
| AACTGGCGAAAATATATTGA+AGG | - | 57448:15917-15936 | MS.gene07867:intron | 30.0% | |
| ATGGCTAGAATTAATGTTGT+GGG | - | 57448:15261-15280 | MS.gene07867:intron | 30.0% | |
| ATTCAGATGAAGAGGATATT+AGG | - | 57448:15364-15383 | MS.gene07867:intron | 30.0% | |
| GCTAGCTAGATAGTATTAAT+CGG | + | 57448:15660-15679 | None:intergenic | 30.0% | |
| GGTTAAAAGGAACATATACT+TGG | - | 57448:16316-16335 | MS.gene07867:intron | 30.0% | |
| TATGTAAGACTTCCTCTATA+AGG | + | 57448:16091-16110 | None:intergenic | 30.0% | |
| TCATATTAAGTAGGACCAAT+AGG | - | 57448:16049-16068 | MS.gene07867:CDS | 30.0% | |
| TCTCAGAAAATGTATAATGG+TGG | - | 57448:16377-16396 | MS.gene07867:intron | 30.0% | |
| ! | TTGAAGGTATATAGTGCTAT+TGG | - | 57448:15933-15952 | MS.gene07867:intron | 30.0% |
| !! | GGTTTGAATTTCAGGTTAAA+AGG | - | 57448:16303-16322 | MS.gene07867:intron | 30.0% |
| AAGAGAACTTGAAATTCAGC+TGG | - | 57448:15853-15872 | MS.gene07867:intron | 35.0% | |
| AATTCTGATGACAATGGTTC+TGG | - | 57448:15297-15316 | MS.gene07867:intron | 35.0% | |
| AGAGAACTTGAAATTCAGCT+GGG | - | 57448:15854-15873 | MS.gene07867:intron | 35.0% | |
| CAGCAAAATTCTGATGACAA+TGG | - | 57448:15291-15310 | MS.gene07867:intron | 35.0% | |
| GAAGAGGATATTAGGACAAA+TGG | - | 57448:15372-15391 | MS.gene07867:intron | 35.0% | |
| GAATAAAGATTGGAGAGCTT+TGG | + | 57448:15503-15522 | None:intergenic | 35.0% | |
| GGATTCATTACAAATCATGC+AGG | + | 57448:15639-15658 | None:intergenic | 35.0% | |
| TATCCAAGATTTCTTGTCTC+TGG | - | 57448:15958-15977 | MS.gene07867:intron | 35.0% | |
| TTTGGTCCCTAGAAATGAAT+TGG | - | 57448:16630-16649 | MS.gene07867:CDS | 35.0% | |
| ! | CTTTTTGCGGTTTGAATTTC+AGG | - | 57448:16295-16314 | MS.gene07867:intron | 35.0% |
| ! | GTTTTCTCATTCAGATGAAG+AGG | - | 57448:15356-15375 | MS.gene07867:intron | 35.0% |
| !! | AACATATACTTGGTGTATGG+TGG | - | 57448:16326-16345 | MS.gene07867:intron | 35.0% |
| !! | AATGGTTCTGGACTAATAGA+AGG | - | 57448:15309-15328 | MS.gene07867:intron | 35.0% |
| !! | AGCTGAATTTCAAGTTCTCT+TGG | + | 57448:15854-15873 | None:intergenic | 35.0% |
| !! | AGGAACATATACTTGGTGTA+TGG | - | 57448:16323-16342 | MS.gene07867:intron | 35.0% |
| GACGAACGAATTTGAGAAGA+GGG | + | 57448:16170-16189 | None:intergenic | 40.0% | |
| GAGGATATTAGGACAAATGG+TGG | - | 57448:15375-15394 | MS.gene07867:intron | 40.0% | |
| TGACGAACGAATTTGAGAAG+AGG | + | 57448:16171-16190 | None:intergenic | 40.0% | |
| !! | AATAGAAGGTTCTGAGACTG+AGG | - | 57448:15323-15342 | MS.gene07867:intron | 40.0% |
| !! | ATATACTTGGTGTATGGTGG+TGG | - | 57448:16329-16348 | MS.gene07867:intron | 40.0% |
| AATTGCGAATGGACAGGAAC+TGG | - | 57448:15900-15919 | MS.gene07867:intron | 45.0% | |
| AGGCCAGAGACAAGAAATCT+TGG | + | 57448:15964-15983 | None:intergenic | 45.0% | |
| ATCCACTGAATCCTCAGATG+TGG | + | 57448:15403-15422 | None:intergenic | 45.0% | |
| GTCTCAAATTGCGAATGGAC+AGG | - | 57448:15894-15913 | MS.gene07867:intron | 45.0% | |
| ! | CAATTTGAGACACCGACAAC+TGG | + | 57448:15885-15904 | None:intergenic | 45.0% |
| ! | TCGGTGTCTCAAATTGCGAA+TGG | - | 57448:15889-15908 | MS.gene07867:intron | 45.0% |
| !! | TGGTGGAAGTGTTGGATTGA+TGG | - | 57448:16346-16365 | MS.gene07867:intron | 45.0% |
| !!! | GGATCGTGTTTTGATGACAG+TGG | - | 57448:16680-16699 | MS.gene07867:CDS | 45.0% |
| AGCCACATCTGAGGATTCAG+TGG | - | 57448:15398-15417 | MS.gene07867:intron | 50.0% | |
| AGCTGGGATGTACCAGTTGT+CGG | - | 57448:15870-15889 | MS.gene07867:intron | 50.0% | |
| CTGAGGATTCAGTGGATCTG+AGG | - | 57448:15406-15425 | MS.gene07867:intron | 50.0% | |
| ! | AAATGGTGGAGCCACATCTG+AGG | - | 57448:15389-15408 | MS.gene07867:intron | 50.0% |
| !! | AGAAGGTTCTGAGACTGAGG+AGG | - | 57448:15326-15345 | MS.gene07867:intron | 50.0% |
| !! | GTGTATGGTGGTGGAAGTGT+TGG | - | 57448:16338-16357 | MS.gene07867:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57448 | gene | 15261 | 16709 | 15261 | ID=MS.gene07867 |
| 57448 | mRNA | 15261 | 16709 | 15261 | ID=MS.gene07867.t1;Parent=MS.gene07867 |
| 57448 | exon | 16543 | 16709 | 16543 | ID=MS.gene07867.t1.exon1;Parent=MS.gene07867.t1 |
| 57448 | CDS | 16543 | 16709 | 16543 | ID=cds.MS.gene07867.t1;Parent=MS.gene07867.t1 |
| 57448 | exon | 16032 | 16170 | 16032 | ID=MS.gene07867.t1.exon2;Parent=MS.gene07867.t1 |
| 57448 | CDS | 16032 | 16170 | 16032 | ID=cds.MS.gene07867.t1;Parent=MS.gene07867.t1 |
| 57448 | exon | 15556 | 15653 | 15556 | ID=MS.gene07867.t1.exon3;Parent=MS.gene07867.t1 |
| 57448 | CDS | 15556 | 15653 | 15556 | ID=cds.MS.gene07867.t1;Parent=MS.gene07867.t1 |
| 57448 | exon | 15261 | 15270 | 15261 | ID=MS.gene07867.t1.exon4;Parent=MS.gene07867.t1 |
| 57448 | CDS | 15261 | 15270 | 15261 | ID=cds.MS.gene07867.t1;Parent=MS.gene07867.t1 |
| Gene Sequence |
| Protein sequence |
