AlfalfaGEDB | MS.gene05265

M. sativa cultivar XinJiangDaYe / MS.gene05265

KEGG: K10727
GO: -
PFAM: PF16679.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05265.t1	KEH26836.1	84.2	120	8	1	2	110	328	447	1.70E-45	191.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05265.t1	Q9M1S9	41.2	85	50	0	19	103	390	474	3.0e-11	69.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05265.t1	A0A072UAU7	84.2	120	8	1	2	110	410	529	1.2e-45	191.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene05264	MS.gene05265	0.813219	2.86E-51	-1.69E-46
MS.gene05265	MS.gene05266	0.879422	1.26E-69	-1.69E-46
MS.gene05265	MS.gene053903	0.802611	5.17E-49	-1.69E-46
MS.gene05265	MS.gene058974	0.821851	3.24E-53	-1.69E-46
MS.gene05265	MS.gene060257	0.82104	4.99E-53	-1.69E-46
MS.gene05265	MS.gene064718	0.800596	1.34E-48	-1.69E-46
MS.gene05265	MS.gene068336	0.801858	7.38E-49	-1.69E-46
MS.gene05265	MS.gene068338	0.819856	9.32E-53	-1.69E-46
MS.gene05265	MS.gene072819	0.811423	7.06E-51	-1.69E-46
MS.gene05265	MS.gene073543	0.807709	4.43E-50	-1.69E-46
MS.gene05265	MS.gene07867	0.832275	1.04E-55	-1.69E-46
MS.gene05265	MS.gene20049	0.839367	1.65E-57	-1.69E-46
MS.gene05265	MS.gene22612	0.816577	5.15E-52	-1.69E-46
MS.gene05265	MS.gene22768	0.80736	5.25E-50	-1.69E-46
MS.gene05265	MS.gene23450	0.806084	9.77E-50	-1.69E-46
MS.gene05265	MS.gene25539	0.80623	9.10E-50	-1.69E-46
MS.gene05265	MS.gene37802	0.800137	1.66E-48	-1.69E-46
MS.gene05265	MS.gene40603	0.814761	1.31E-51	-1.69E-46
MS.gene05265	MS.gene45098	0.807445	5.04E-50	-1.69E-46
MS.gene05265	MS.gene47617	0.800166	1.64E-48	-1.69E-46
MS.gene05265	MS.gene47623	0.830439	2.94E-55	-1.69E-46
MS.gene05265	MS.gene48626	0.808257	3.39E-50	-1.69E-46
MS.gene05265	MS.gene49924	0.812363	4.40E-51	-1.69E-46
MS.gene05265	MS.gene49949	0.814033	1.89E-51	-1.69E-46
MS.gene05265	MS.gene50905	0.823652	1.23E-53	-1.69E-46
MS.gene05265	MS.gene53827	0.817196	3.74E-52	-1.69E-46
MS.gene05265	MS.gene58485	0.822955	1.80E-53	-1.69E-46
MS.gene05265	MS.gene58638	0.807961	3.91E-50	-1.69E-46
MS.gene05265	MS.gene59553	0.80334	3.65E-49	-1.69E-46
MS.gene05265	MS.gene61388	0.80025	1.57E-48	-1.69E-46
MS.gene05265	MS.gene61424	0.849144	3.90E-60	-1.69E-46
MS.gene05265	MS.gene61425	0.820617	6.24E-53	-1.69E-46
MS.gene05265	MS.gene76954	0.804081	2.56E-49	-1.69E-46
MS.gene05265	MS.gene88533	0.806178	9.33E-50	-1.69E-46
MS.gene05265	MS.gene88539	0.803852	2.86E-49	-1.69E-46
MS.gene05265	MS.gene92276	0.826302	2.92E-54	-1.69E-46
MS.gene05265	MS.gene93663	0.835138	2.00E-56	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05265.t1	MTR_6g477680	84.167	120	8	1	2	110	328	447	8.38e-63	197
MS.gene05265.t1	MTR_6g077680	84.167	120	8	1	2	110	410	529	6.33e-62	197
MS.gene05265.t1	MTR_6g077630	79.675	123	12	2	1	110	41	163	5.63e-60	181
MS.gene05265.t1	MTR_6g079130	79.675	123	12	2	1	110	93	215	1.01e-58	180
MS.gene05265.t1	MTR_6g077680	80.583	103	9	1	2	93	410	512	1.20e-48	164
MS.gene05265.t1	MTR_6g077630	78.218	101	9	2	1	88	41	141	3.75e-46	146
MS.gene05265.t1	MTR_6g078940	58.000	100	15	1	10	109	49	121	3.09e-28	99.8
MS.gene05265.t1	MTR_6g078920	72.059	68	7	2	2	58	136	202	4.03e-21	84.0
MS.gene05265.t1	MTR_6g078950	80.488	41	8	0	60	100	66	106	5.85e-17	74.7
MS.gene05265.t1	MTR_6g078990	62.264	53	9	1	1	42	192	244	9.60e-11	57.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05265.t1	AT3G54710	41.667	84	49	0	20	103	391	474	1.37e-14	68.6
MS.gene05265.t1	AT2G31270	39.286	84	51	0	20	103	475	558	2.42e-13	65.1

Find 20 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGAAGAACAGATTGAGATTT+TGG	0.155235	6.2:-78367492	MS.gene05265:CDS
TGCAAGAAGTTGGTTTCTTC+TGG	0.236168	6.2:-78367446	MS.gene05265:CDS
CCGATGCAACCAAACAATTA+AGG	0.258730	6.2:+78367740	None:intergenic
TCCTTCATCATTGGTGGAAC+TGG	0.336190	6.2:-78367710	MS.gene05265:CDS
TGTCAAGAATGCGTTAGATT+TGG	0.348270	6.2:-78367165	MS.gene05265:CDS
AGATGAGAACCTTAATTGTT+TGG	0.373771	6.2:-78367749	MS.gene05265:CDS
AAGAACTTCAATCACTAAAG+AGG	0.410853	6.2:-78367653	MS.gene05265:CDS
AACTTCTTGCAAAGCCAGTC+TGG	0.423623	6.2:+78367458	None:intergenic
TGGAAAAGACTGTGCCAGAC+TGG	0.439177	6.2:-78367472	MS.gene05265:CDS
CCTTAATTGTTTGGTTGCAT+CGG	0.448458	6.2:-78367740	MS.gene05265:CDS
AGACTGGCTTTGCAAGAAGT+TGG	0.482751	6.2:-78367456	MS.gene05265:CDS
GGATAACAGTCCTTCATCAT+TGG	0.503210	6.2:-78367719	MS.gene05265:CDS
AGAACTTCAATCACTAAAGA+GGG	0.518710	6.2:-78367652	MS.gene05265:CDS
TTCTCAGCAATGTCAACAAA+GGG	0.519094	6.2:-78367124	MS.gene05265:CDS
ACCAGTTCCACCAATGATGA+AGG	0.537388	6.2:+78367709	None:intergenic
TAACAGTCCTTCATCATTGG+TGG	0.552963	6.2:-78367716	MS.gene05265:CDS
CTTCTGGAGACACAATGCAC+TGG	0.579065	6.2:-78367430	MS.gene05265:intron
CTTCTCAGCAATGTCAACAA+AGG	0.594149	6.2:-78367125	MS.gene05265:CDS
ATTTGGACTCAGTTCGATCG+AGG	0.618618	6.2:-78367148	MS.gene05265:CDS
GAACTTCAATCACTAAAGAG+GGG	0.668659	6.2:-78367651	MS.gene05265:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TGGAATTTCATGTTTTAAGT+TGG	+	chr6.2:78367548-78367567	None:intergenic	25.0%
	AAGAACTTCAATCACTAAAG+AGG	-	chr6.2:78367222-78367241	MS.gene05265:intron	30.0%
	AGAACTTCAATCACTAAAGA+GGG	-	chr6.2:78367223-78367242	MS.gene05265:intron	30.0%
	AGATGAGAACCTTAATTGTT+TGG	-	chr6.2:78367126-78367145	MS.gene05265:CDS	30.0%
	ATTCCATTATATCCCAAAGA+AGG	+	chr6.2:78367572-78367591	None:intergenic	30.0%
!	TGAAGAACAGATTGAGATTT+TGG	-	chr6.2:78367383-78367402	MS.gene05265:intron	30.0%
	ATGAAATTCCAGCCTTCTTT+GGG	-	chr6.2:78367557-78367576	MS.gene05265:intron	35.0%
	CCTTAATTGTTTGGTTGCAT+CGG	-	chr6.2:78367135-78367154	MS.gene05265:CDS	35.0%
	CTTGACACTGCAAATATAAC+AGG	+	chr6.2:78367697-78367716	None:intergenic	35.0%
	GAACTTCAATCACTAAAGAG+GGG	-	chr6.2:78367224-78367243	MS.gene05265:intron	35.0%
	TATTAGTAGCCAAAATCCCT+TGG	-	chr6.2:78367609-78367628	MS.gene05265:CDS	35.0%
	TGTCAAGAATGCGTTAGATT+TGG	-	chr6.2:78367710-78367729	MS.gene05265:CDS	35.0%
	TTCTCAGCAATGTCAACAAA+GGG	-	chr6.2:78367751-78367770	MS.gene05265:CDS	35.0%
!	CTGCTACTCTAAAAGAACAA+AGG	+	chr6.2:78367497-78367516	None:intergenic	35.0%
	ACTAATCTTGTCCTGCCAAT+AGG	-	chr6.2:78367354-78367373	MS.gene05265:intron	40.0%
	CATGAAATTCCAGCCTTCTT+TGG	-	chr6.2:78367556-78367575	MS.gene05265:intron	40.0%
	CATTATATCCCAAAGAAGGC+TGG	+	chr6.2:78367568-78367587	None:intergenic	40.0%
	CCGATGCAACCAAACAATTA+AGG	+	chr6.2:78367138-78367157	None:intergenic	40.0%
	CTTCTCAGCAATGTCAACAA+AGG	-	chr6.2:78367750-78367769	MS.gene05265:CDS	40.0%
	GGATAACAGTCCTTCATCAT+TGG	-	chr6.2:78367156-78367175	MS.gene05265:CDS	40.0%
	TAACAGTCCTTCATCATTGG+TGG	-	chr6.2:78367159-78367178	MS.gene05265:CDS	40.0%
	TGTTCTTCAGCTTCTCCTAT+TGG	+	chr6.2:78367372-78367391	None:intergenic	40.0%
!	TAGTTTGAAGTCAACGCCAA+GGG	+	chr6.2:78367628-78367647	None:intergenic	40.0%
!	TGCAAGAAGTTGGTTTCTTC+TGG	-	chr6.2:78367429-78367448	MS.gene05265:intron	40.0%
!!	CAGCCTTCTTTGGGATATAA+TGG	-	chr6.2:78367566-78367585	MS.gene05265:intron	40.0%
	AACTTCTTGCAAAGCCAGTC+TGG	+	chr6.2:78367420-78367439	None:intergenic	45.0%
	ACCAGTTCCACCAATGATGA+AGG	+	chr6.2:78367169-78367188	None:intergenic	45.0%
	ATTTGGACTCAGTTCGATCG+AGG	-	chr6.2:78367727-78367746	MS.gene05265:CDS	45.0%
	TCCTTCATCATTGGTGGAAC+TGG	-	chr6.2:78367165-78367184	MS.gene05265:CDS	45.0%
!	AAGTCAACGCCAAGGGATTT+TGG	+	chr6.2:78367621-78367640	None:intergenic	45.0%
!	CTAGTTTGAAGTCAACGCCA+AGG	+	chr6.2:78367629-78367648	None:intergenic	45.0%
!!	AGACTGGCTTTGCAAGAAGT+TGG	-	chr6.2:78367419-78367438	MS.gene05265:intron	45.0%
	CTTCAGCTTCTCCTATTGGC+AGG	+	chr6.2:78367368-78367387	None:intergenic	50.0%
	TGGAAAAGACTGTGCCAGAC+TGG	-	chr6.2:78367403-78367422	MS.gene05265:intron	50.0%
!	CTTCTGGAGACACAATGCAC+TGG	-	chr6.2:78367445-78367464	MS.gene05265:CDS	50.0%
!	TGGAGACACAATGCACTGGT+GGG	-	chr6.2:78367449-78367468	MS.gene05265:CDS	50.0%
	CTGGAGACACAATGCACTGG+TGG	-	chr6.2:78367448-78367467	MS.gene05265:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.2	gene	78367118	78367779	78367118	ID=MS.gene05265
chr6.2	mRNA	78367118	78367779	78367118	ID=MS.gene05265.t1;Parent=MS.gene05265
chr6.2	exon	78367605	78367779	78367605	ID=MS.gene05265.t1.exon1;Parent=MS.gene05265.t1
chr6.2	CDS	78367605	78367779	78367605	ID=cds.MS.gene05265.t1;Parent=MS.gene05265.t1
chr6.2	exon	78367431	78367521	78367431	ID=MS.gene05265.t1.exon2;Parent=MS.gene05265.t1
chr6.2	CDS	78367431	78367521	78367431	ID=cds.MS.gene05265.t1;Parent=MS.gene05265.t1
chr6.2	exon	78367118	78367187	78367118	ID=MS.gene05265.t1.exon3;Parent=MS.gene05265.t1
chr6.2	CDS	78367118	78367187	78367118	ID=cds.MS.gene05265.t1;Parent=MS.gene05265.t1

Gene Sequence

>MS.gene05265

ATGCAGGTAGATGAGAACCTTAATTGTTTGGTTGCATCGGATAACAGTCCTTCATCATTGGTGGAACTGGTTAATGTGATTGACTCTATATTTGACTCTGTCAAAAGAACTTCAATCACTAAAGAGGGGCTCTTGCAAAAGATAATGATGAACTGTTTAGATTTTGTTGAGATTAGTATGTTCTAATTCTATTTACTATAATGTACACGTTTTCTTTGTAATAAACTTTCTAGCTAACTAATCTTGTCCTGCCAATAGGAGAAGCTGAAGAACAGATTGAGATTTTGGAAAAGACTGTGCCAGACTGGCTTTGCAAGAAGTTGGTTTCTTCTGGAGACACAATGCACTGGTGGGTCTCTTTCTTTTTATGATTAAACCTTTGTTCTTTTAGAGTAGCAGTCTCTTTTAGAGATGTGAAAATTACAGTCCAACTTAAAACATGAAATTCCAGCCTTCTTTGGGATATAATGGAATAGCAGAACTTTAGATGTTATTAGTAGCCAAAATCCCTTGGCGTTGACTTCAAACTAGTTATTTTATTTGTTCTCTGTGTTCTTAAGCTTGTAGTTTATAAAACCTGTTATATTTGCAGTGTCAAGAATGCGTTAGATTTGGACTCAGTTCGATCGAGGCTTCTCAGCAATGTCAACAAAGGGAGATGA

Protein sequence

>MS.gene05265.t1

MQVDENLNCLVASDNSPSSLVELVNVIDSIFDSVKRTSITKEGLLQKIMMNCLDFVEIREAEEQIEILEKTVPDWLCKKLVSSGDTMHCVKNALDLDSVRSRLLSNVNKGR