AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07909


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07909.t1 MTR_8g020700 91.803 183 15 0 1 183 26 208 1.14e-121 344
MS.gene07909.t1 MTR_8g020700 91.803 183 15 0 1 183 26 208 5.80e-121 346
MS.gene07909.t1 MTR_8g020690 78.689 183 39 0 1 183 26 208 1.09e-104 305
MS.gene07909.t1 MTR_3g467270 71.271 181 52 0 3 183 27 207 4.65e-97 286
MS.gene07909.t1 MTR_8g076900 72.376 181 50 0 3 183 28 208 5.55e-96 279
MS.gene07909.t1 MTR_4g018820 71.823 181 51 0 3 183 28 208 1.78e-95 281
MS.gene07909.t1 MTR_8g076900 74.011 177 46 0 7 183 32 208 1.57e-94 279
MS.gene07909.t1 MTR_6g056070 68.508 181 57 0 3 183 29 209 2.28e-90 268
MS.gene07909.t1 MTR_8g077040 68.508 181 57 0 3 183 28 208 5.72e-90 268
MS.gene07909.t1 MTR_5g020940 62.637 182 67 1 3 183 38 219 1.55e-85 256
MS.gene07909.t1 MTR_8g077030 56.667 180 56 2 4 183 29 186 3.18e-63 203
MS.gene07909.t1 MTR_3g117060 50.276 181 84 1 7 181 32 212 3.16e-62 197
MS.gene07909.t1 MTR_5g046110 45.745 188 96 1 2 183 87 274 9.98e-52 171
MS.gene07909.t1 MTR_3g467560 46.629 178 89 2 10 181 59 236 2.29e-50 167
MS.gene07909.t1 MTR_7g084620 46.154 182 87 4 7 180 32 210 1.99e-46 156
MS.gene07909.t1 MTR_7g084350 41.848 184 101 2 3 180 26 209 4.35e-45 152
MS.gene07909.t1 MTR_1g022455 46.471 170 78 3 27 183 55 224 1.90e-39 138
MS.gene07909.t1 MTR_1g022465 43.878 196 94 4 4 183 29 224 1.82e-38 135
MS.gene07909.t1 MTR_1g108660 42.857 168 77 4 20 181 33 187 1.94e-36 129
MS.gene07909.t1 MTR_7g084330 39.326 178 97 5 10 179 18 192 1.84e-33 121
MS.gene07909.t1 MTR_3g054330 37.888 161 91 3 29 180 63 223 5.65e-31 115
MS.gene07909.t1 MTR_5g093900 38.788 165 91 4 25 180 69 232 1.82e-29 112
MS.gene07909.t1 MTR_3g052700 37.805 164 94 3 25 180 67 230 2.49e-29 111
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07909.t1 AT2G14060 47.879 165 83 1 18 182 49 210 5.34e-52 167
MS.gene07909.t1 AT5G04380 47.778 180 88 2 3 181 53 227 1.34e-51 169
MS.gene07909.t1 AT5G04380 51.592 157 71 1 25 181 9 160 1.49e-51 168
MS.gene07909.t1 AT5G04380 47.778 180 88 2 3 181 57 231 1.57e-51 170
MS.gene07909.t1 AT2G14060 48.171 164 82 1 18 181 49 209 9.23e-51 167
MS.gene07909.t1 AT3G21950 49.677 155 75 1 27 181 50 201 1.55e-50 167
MS.gene07909.t1 AT3G21950 50.327 153 73 1 27 179 50 199 2.90e-50 167
MS.gene07909.t1 AT5G04370 51.923 156 69 2 27 181 85 235 2.95e-50 167
MS.gene07909.t1 AT5G38020 46.369 179 92 2 4 181 26 201 5.03e-50 165
MS.gene07909.t1 AT5G04370 51.923 156 69 2 27 181 90 240 5.62e-50 166
MS.gene07909.t1 AT5G04370 51.923 156 69 2 27 181 104 254 5.62e-50 166
MS.gene07909.t1 AT5G38020 50.323 155 74 1 27 181 11 162 1.37e-49 163
MS.gene07909.t1 AT5G66430 47.742 155 76 1 27 181 45 194 1.63e-49 164
MS.gene07909.t1 AT3G11480 49.032 155 76 1 27 181 75 226 9.48e-49 162
MS.gene07909.t1 AT4G36470 45.078 193 89 4 2 181 30 218 5.08e-45 152
MS.gene07909.t1 AT1G19640 43.500 200 95 4 2 183 27 226 9.53e-39 136
MS.gene07909.t1 AT1G68040 41.818 165 79 3 24 183 58 210 4.76e-37 131
MS.gene07909.t1 AT3G44840 44.693 179 79 6 12 183 35 200 5.87e-34 123
MS.gene07909.t1 AT4G26420 37.696 191 99 7 4 183 33 214 2.73e-32 116
MS.gene07909.t1 AT4G26420 37.696 191 99 7 4 183 21 202 4.22e-32 116
MS.gene07909.t1 AT4G26420 37.696 191 99 7 4 183 33 214 3.39e-31 116
MS.gene07909.t1 AT3G44860 42.021 188 89 6 1 181 24 198 3.52e-31 115
MS.gene07909.t1 AT5G38100 42.515 167 71 7 29 183 58 211 3.73e-31 113
MS.gene07909.t1 AT5G38100 42.515 167 71 7 29 183 58 211 3.86e-31 113
MS.gene07909.t1 AT5G38780 40.964 166 78 5 28 183 9 164 1.59e-30 113
MS.gene07909.t1 AT5G38100 42.515 167 71 7 29 183 35 188 2.25e-30 113
MS.gene07909.t1 AT5G38780 40.964 166 78 5 28 183 58 213 2.88e-30 114
MS.gene07909.t1 AT5G37970 39.594 197 92 7 3 183 29 214 3.71e-30 113
MS.gene07909.t1 AT5G38100 42.515 167 71 7 29 183 58 211 4.46e-30 113
MS.gene07909.t1 AT3G44870 39.572 187 92 6 1 181 24 195 1.14e-29 112
MS.gene07909.t1 AT5G55250 36.025 161 95 3 31 183 32 192 1.22e-29 112
MS.gene07909.t1 AT5G55250 36.025 161 95 3 31 183 70 230 2.10e-29 112
MS.gene07909.t1 AT1G15125 39.752 161 78 5 29 182 48 196 2.39e-29 108
MS.gene07909.t1 AT1G66700 36.898 187 95 5 7 183 32 205 1.26e-28 107
MS.gene07909.t1 AT1G15125 39.752 161 78 5 29 182 48 196 1.88e-28 108
MS.gene07909.t1 AT1G66700 36.898 187 95 5 7 183 32 205 3.43e-28 108
MS.gene07909.t1 AT5G37990 38.579 197 94 8 3 183 29 214 7.97e-28 107
MS.gene07909.t1 AT5G56300 33.862 189 110 5 3 183 43 224 6.92e-27 105
MS.gene07909.t1 AT1G66720 36.207 174 91 4 16 181 42 203 7.50e-27 104
MS.gene07909.t1 AT1G66690 36.158 177 90 5 13 179 38 201 2.49e-24 97.8

Find 34 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTATGGCTTCATCTCTTAT+TGG 0.171047 8.3:-70461569 None:intergenic
AAATGAAATCAAAACTAAAA+TGG 0.175532 8.3:+70461820 MS.gene07909:CDS
ATGGGTCCTTGTTACTTCTT+TGG 0.197736 8.3:+70461839 MS.gene07909:CDS
TGTTACTTCTTTGGGGTTCC+TGG 0.212711 8.3:+70461848 MS.gene07909:CDS
AACCTTGCAATTGCAGATTT+AGG 0.230846 8.3:+70461620 MS.gene07909:CDS
TGGGTCCTTGTTACTTCTTT+GGG 0.234467 8.3:+70461840 MS.gene07909:CDS
GCAGATTTAGGTTGTTCTTC+TGG 0.263073 8.3:+70461632 MS.gene07909:CDS
TATAGGGAAATGCAGTTTCT+TGG 0.287192 8.3:+70461540 None:intergenic
TCAATTTAAAAGAGACTTCT+CGG 0.358277 8.3:+70462337 MS.gene07909:CDS
GAGATGAATGGTTCAATTGT+TGG 0.392209 8.3:-70461709 None:intergenic
ATTCCTCTTACAGCCTTCAT+TGG 0.426219 8.3:+70461918 MS.gene07909:CDS
TAGAAAGTGTGTTGGAGTAA+AGG 0.429689 8.3:-70461595 None:intergenic
AATGAAATCAAAACTAAAAT+GGG 0.439241 8.3:+70461821 MS.gene07909:CDS
TATGCTTTAACAACATTTGA+GGG 0.450868 8.3:-70462308 None:intergenic
ATGAAATGCAAACTTTGATC+AGG 0.468189 8.3:-70461893 None:intergenic
TTTGGTATCAGACATTATCA+TGG 0.479161 8.3:+70461670 MS.gene07909:CDS
GTTGGAGTAAAGGCTTGTTA+TGG 0.479472 8.3:-70461585 None:intergenic
CAGCCTTCATTGGCTATCAA+AGG 0.487208 8.3:+70461928 MS.gene07909:CDS
GTATGCTTTAACAACATTTG+AGG 0.517154 8.3:-70462309 None:intergenic
GGGTCCTTGTTACTTCTTTG+GGG 0.519835 8.3:+70461841 MS.gene07909:CDS
TAGCCAATGAAGGCTGTAAG+AGG 0.535843 8.3:-70461921 None:intergenic
CGTGCAGAGGAACTAGTTGA+AGG 0.536965 8.3:+70462374 MS.gene07909:CDS
TAACCTTTGATAGCCAATGA+AGG 0.548055 8.3:-70461931 None:intergenic
TCTTGTACTCGGGAGATGAA+TGG 0.553449 8.3:-70461721 None:intergenic
GTGCAGAGGAACTAGTTGAA+GGG 0.554125 8.3:+70462375 MS.gene07909:CDS
AACCTAAATCTGCAATTGCA+AGG 0.554611 8.3:-70461622 None:intergenic
TGTGTAGTTGATGTAAATGT+TGG 0.562245 8.3:-70462282 None:intergenic
TTATTGTTCTCTATACCCTC+CGG 0.563075 8.3:-70462257 None:intergenic
TGCAGAGGAACTAGTTGAAG+GGG 0.594526 8.3:+70462376 MS.gene07909:CDS
GGGAATTATTAGGTTCCGGA+GGG 0.604890 8.3:+70462242 MS.gene07909:intron
GGGGAATTATTAGGTTCCGG+AGG 0.634746 8.3:+70462241 MS.gene07909:intron
GGAACCCCAAAGAAGTAACA+AGG 0.641016 8.3:-70461845 None:intergenic
ACTCTGTTATAGAAAGAACC+AGG 0.668917 8.3:-70461866 None:intergenic
GCAGAGGAACTAGTTGAAGG+GGG 0.710839 8.3:+70462377 MS.gene07909:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTAATGTTTTTTTTTTTT+TGG - chr8.3:70462188-70462207 None:intergenic 10.0%
!!! TGATTTTATTTGTTAATTAT+GGG + chr8.3:70462221-70462240 MS.gene07909:intron 10.0%
!!! TTGATTTTATTTGTTAATTA+TGG + chr8.3:70462220-70462239 MS.gene07909:intron 10.0%
!! AAATGAAATCAAAACTAAAA+TGG + chr8.3:70461820-70461839 MS.gene07909:CDS 15.0%
!! AATGAAATCAAAACTAAAAT+GGG + chr8.3:70461821-70461840 MS.gene07909:CDS 15.0%
!!! GATTTTATTTGTTAATTATG+GGG + chr8.3:70462222-70462241 MS.gene07909:intron 15.0%
!! ATTGAAAAATATCTTGTACT+CGG - chr8.3:70461735-70461754 None:intergenic 20.0%
!! GAAACACAATATAAAATATG+AGG - chr8.3:70461966-70461985 None:intergenic 20.0%
!!! ATATTTTTCAATGATGTTTC+AGG + chr8.3:70461743-70461762 MS.gene07909:CDS 20.0%
! GTTAATTATGGGGAATTATT+AGG + chr8.3:70462232-70462251 MS.gene07909:intron 25.0%
! TACCAATTGAACTACATTTA+CGG - chr8.3:70462067-70462086 None:intergenic 25.0%
! TATGCTTTAACAACATTTGA+GGG - chr8.3:70462311-70462330 None:intergenic 25.0%
! TCAATTTAAAAGAGACTTCT+CGG + chr8.3:70462337-70462356 MS.gene07909:CDS 25.0%
! TTGAAAAATATCTTGTACTC+GGG - chr8.3:70461734-70461753 None:intergenic 25.0%
ACCAATTGAACTACATTTAC+GGG - chr8.3:70462066-70462085 None:intergenic 30.0%
AGATTCACACATATTATGTG+TGG - chr8.3:70462147-70462166 None:intergenic 30.0%
ATGAAATGCAAACTTTGATC+AGG - chr8.3:70461896-70461915 None:intergenic 30.0%
GTATGCTTTAACAACATTTG+AGG - chr8.3:70462312-70462331 None:intergenic 30.0%
TTCCAGGGATAATTTATACA+GGG + chr8.3:70462089-70462108 MS.gene07909:intron 30.0%
TTTGGTATCAGACATTATCA+TGG + chr8.3:70461670-70461689 MS.gene07909:CDS 30.0%
! GATACCAAAAACAAAGTGTT+TGG - chr8.3:70461659-70461678 None:intergenic 30.0%
! TGTGTAGTTGATGTAAATGT+TGG - chr8.3:70462285-70462304 None:intergenic 30.0%
!! AAGGTTTTTAGAAAGTGTGT+TGG - chr8.3:70461606-70461625 None:intergenic 30.0%
AACCTAAATCTGCAATTGCA+AGG - chr8.3:70461625-70461644 None:intergenic 35.0%
AACCTTGCAATTGCAGATTT+AGG + chr8.3:70461620-70461639 MS.gene07909:CDS 35.0%
ACATATTATGTGTGGAGAAG+TGG - chr8.3:70462139-70462158 None:intergenic 35.0%
ACTCTGTTATAGAAAGAACC+AGG - chr8.3:70461869-70461888 None:intergenic 35.0%
ATATTATGTGTGGAGAAGTG+GGG - chr8.3:70462137-70462156 None:intergenic 35.0%
CATATTATGTGTGGAGAAGT+GGG - chr8.3:70462138-70462157 None:intergenic 35.0%
CTTCCAGGGATAATTTATAC+AGG + chr8.3:70462088-70462107 MS.gene07909:intron 35.0%
GAGATGAATGGTTCAATTGT+TGG - chr8.3:70461712-70461731 None:intergenic 35.0%
GTTATGGCTTCATCTCTTAT+TGG - chr8.3:70461572-70461591 None:intergenic 35.0%
TATGGGGAATTATTAGGTTC+CGG + chr8.3:70462238-70462257 MS.gene07909:intron 35.0%
TATGTGTGAATCTAGTCACT+AGG + chr8.3:70462154-70462173 MS.gene07909:intron 35.0%
TCCAGGGATAATTTATACAG+GGG + chr8.3:70462090-70462109 MS.gene07909:intron 35.0%
TCCCGTAAATGTAGTTCAAT+TGG + chr8.3:70462062-70462081 MS.gene07909:intron 35.0%
TTATTGTTCTCTATACCCTC+CGG - chr8.3:70462260-70462279 None:intergenic 35.0%
! TAACCTTTGATAGCCAATGA+AGG - chr8.3:70461934-70461953 None:intergenic 35.0%
! TAGAAAGTGTGTTGGAGTAA+AGG - chr8.3:70461598-70461617 None:intergenic 35.0%
!!! TGGACCAAACACTTTGTTTT+TGG + chr8.3:70461652-70461671 MS.gene07909:CDS 35.0%
ATTCCTCTTACAGCCTTCAT+TGG + chr8.3:70461918-70461937 MS.gene07909:CDS 40.0%
GATAATTTATACAGGGGCCA+GGG + chr8.3:70462096-70462115 MS.gene07909:intron 40.0%
! AGTTCAATTGGTAGCTTCCA+GGG + chr8.3:70462074-70462093 MS.gene07909:intron 40.0%
! ATGGGTCCTTGTTACTTCTT+TGG + chr8.3:70461839-70461858 MS.gene07909:CDS 40.0%
! TAGTTCAATTGGTAGCTTCC+AGG + chr8.3:70462073-70462092 MS.gene07909:intron 40.0%
! TGGGTCCTTGTTACTTCTTT+GGG + chr8.3:70461840-70461859 MS.gene07909:CDS 40.0%
!! GCAGATTTAGGTTGTTCTTC+TGG + chr8.3:70461632-70461651 MS.gene07909:CDS 40.0%
!! GTTGGAGTAAAGGCTTGTTA+TGG - chr8.3:70461588-70461607 None:intergenic 40.0%
!! TTTTCTCAAGTATCGTGCAG+AGG + chr8.3:70462361-70462380 MS.gene07909:CDS 40.0%
GCCCCTGTATAAATTATCCC+TGG - chr8.3:70462094-70462113 None:intergenic 45.0%
GGAACCCCAAAGAAGTAACA+AGG - chr8.3:70461848-70461867 None:intergenic 45.0%
GGATAATTTATACAGGGGCC+AGG + chr8.3:70462095-70462114 MS.gene07909:intron 45.0%
GGGAATTATTAGGTTCCGGA+GGG + chr8.3:70462242-70462261 MS.gene07909:intron 45.0%
GTGCAGAGGAACTAGTTGAA+GGG + chr8.3:70462375-70462394 MS.gene07909:CDS 45.0%
TAGCCAATGAAGGCTGTAAG+AGG - chr8.3:70461924-70461943 None:intergenic 45.0%
TCTTGTACTCGGGAGATGAA+TGG - chr8.3:70461724-70461743 None:intergenic 45.0%
TGCAGAGGAACTAGTTGAAG+GGG + chr8.3:70462376-70462395 MS.gene07909:CDS 45.0%
! CAGCCTTCATTGGCTATCAA+AGG + chr8.3:70461928-70461947 MS.gene07909:CDS 45.0%
! GGGTCCTTGTTACTTCTTTG+GGG + chr8.3:70461841-70461860 MS.gene07909:CDS 45.0%
!! TGTTACTTCTTTGGGGTTCC+TGG + chr8.3:70461848-70461867 MS.gene07909:CDS 45.0%
CAGAGGAACTAGTTGAAGGG+GGG + chr8.3:70462378-70462397 MS.gene07909:CDS 50.0%
CGTGCAGAGGAACTAGTTGA+AGG + chr8.3:70462374-70462393 MS.gene07909:CDS 50.0%
GCAGAGGAACTAGTTGAAGG+GGG + chr8.3:70462377-70462396 MS.gene07909:CDS 50.0%
GGGGAATTATTAGGTTCCGG+AGG + chr8.3:70462241-70462260 MS.gene07909:intron 50.0%
GTGTGGAGAAGTGGGGAATC+CGG - chr8.3:70462130-70462149 None:intergenic 55.0%
TGTGGAGAAGTGGGGAATCC+GGG - chr8.3:70462129-70462148 None:intergenic 55.0%
GTGGAGAAGTGGGGAATCCG+GGG - chr8.3:70462128-70462147 None:intergenic 60.0%
GGAATCCGGGGTTCGAACCC+TGG - chr8.3:70462116-70462135 None:intergenic 65.0%
AGGGGCCAGGGTTCGAACCC+CGG + chr8.3:70462108-70462127 MS.gene07909:intron 70.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 70461545 70462398 70461545 ID=MS.gene07909
chr8.3 mRNA 70461545 70462398 70461545 ID=MS.gene07909.t1;Parent=MS.gene07909
chr8.3 exon 70461545 70461949 70461545 ID=MS.gene07909.t1.exon1;Parent=MS.gene07909.t1
chr8.3 CDS 70461545 70461949 70461545 ID=cds.MS.gene07909.t1;Parent=MS.gene07909.t1
chr8.3 exon 70462254 70462398 70462254 ID=MS.gene07909.t1.exon2;Parent=MS.gene07909.t1
chr8.3 CDS 70462254 70462398 70462254 ID=cds.MS.gene07909.t1;Parent=MS.gene07909.t1
Gene Sequence

>MS.gene07909

GGAAATGCAGTTTCTTGGACAAAGCCAATAAGAGATGAAGCCATAACAAGCCTTTACTCCAACACACTTTCTAAAAACCTTGCAATTGCAGATTTAGGTTGTTCTTCTGGACCAAACACTTTGTTTTTGGTATCAGACATTATCATGGTTGTTGAGAAGCTTTGCCAACAATTGAACCATTCATCTCCCGAGTACAAGATATTTTTCAATGATGTTTCAGGAAATGATTTCAACAACATATTTAAGTCACTTGACAACTTTAAAGAGAAATTGCAAAATGAAATCAAAACTAAAATGGGTCCTTGTTACTTCTTTGGGGTTCCTGGTTCTTTCTATAACAGAGTTTTTCCTGATCAAAGTTTGCATTTCATTCATTCCTCTTACAGCCTTCATTGGCTATCAAAGGTTAATATTGTTACCTCATATTTTATATTGTGTTTCATGTATATTTTTTAGATTATTTATTTATTTATTTTGAAAAATTTATTCTATAGATGTAAACATTACATGCAAATAGTCCCGTAAATGTAGTTCAATTGGTAGCTTCCAGGGATAATTTATACAGGGGCCAGGGTTCGAACCCCGGATTCCCCACTTCTCCACACATAATATGTGTGAATCTAGTCACTAGGCTACATGACCAAAAAAAAAAAAACATTACATGCAAATATATAATTGATTTTATTTGTTAATTATGGGGAATTATTAGGTTCCGGAGGGTATAGAGAACAATAAGACCAACATTTACATCAACTACACAAGTCCCTCAAATGTTGTTAAAGCATACTATGATCAATTTAAAAGAGACTTCTCGGTTTTTCTCAAGTATCGTGCAGAGGAACTAGTTGAAGGGG

Protein sequence

>MS.gene07909.t1

GNAVSWTKPIRDEAITSLYSNTLSKNLAIADLGCSSGPNTLFLVSDIIMVVEKLCQQLNHSSPEYKIFFNDVSGNDFNNIFKSLDNFKEKLQNEIKTKMGPCYFFGVPGSFYNRVFPDQSLHFIHSSYSLHWLSKVPEGIENNKTNIYINYTSPSNVVKAYYDQFKRDFSVFLKYRAEELVEG