Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07991.t1 | XP_003592222.1 | 87.2 | 39 | 5 | 0 | 54 | 92 | 658 | 696 | 1.80E-09 | 72 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07991.t1 | G7IEP0 | 87.2 | 39 | 5 | 0 | 54 | 92 | 658 | 696 | 1.3e-09 | 72.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055056 | MS.gene07991 | 0.80062 | 1.32E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07991.t1 | MTR_1g100250 | 87.179 | 39 | 5 | 0 | 54 | 92 | 658 | 696 | 1.63e-16 | 73.9 |
| MS.gene07991.t1 | MTR_5g010870 | 86.111 | 36 | 5 | 0 | 16 | 51 | 174 | 209 | 2.03e-15 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 96 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCTTCAGCGAGTGAATTAT+TGG | 0.156036 | 6.3:-32471367 | MS.gene07991:intron |
| TTGGATCACCCTGTTGTAAA+AGG | 0.251128 | 6.3:-32471348 | MS.gene07991:CDS |
| TGGTGCACTTCCGATAATTC+AGG | 0.313629 | 6.3:-32472422 | MS.gene07991:intron |
| GTGAATTGAACCTGAATTAT+CGG | 0.345144 | 6.3:+32472412 | None:intergenic |
| GCATATCAGGAGGAAATTAA+AGG | 0.358920 | 6.3:-32470676 | None:intergenic |
| TTGGTGTTCTTTGTGTTTAG+TGG | 0.378396 | 6.3:-32472026 | MS.gene07991:CDS |
| AATGAGATTGAAGGCATATC+AGG | 0.385642 | 6.3:-32470689 | MS.gene07991:CDS |
| TGTAAAAGGTGCTACACCTT+TGG | 0.393244 | 6.3:-32471334 | MS.gene07991:CDS |
| GAAGCTTCGGGAAGCATAAT+AGG | 0.417470 | 6.3:+32471306 | None:intergenic |
| AAAATAGGGTCTGAAGCTTC+GGG | 0.427083 | 6.3:+32471294 | None:intergenic |
| GATCGGCATCTGTCCCCTCC+TGG | 0.445248 | 6.3:+32471952 | None:intergenic |
| AACATCATCATTTACTTGAT+CGG | 0.487745 | 6.3:+32471935 | None:intergenic |
| GATATAGAGCACAAACAAGA+TGG | 0.503558 | 6.3:-32471987 | MS.gene07991:CDS |
| TTTAGTGGTCAAGTGTTTAG+TGG | 0.505324 | 6.3:-32472011 | MS.gene07991:CDS |
| TTATCAGTAAATGAGATTGA+AGG | 0.547599 | 6.3:-32470698 | MS.gene07991:CDS |
| TACACATGGAACAAAAGCTC+TGG | 0.547970 | 6.3:-32471265 | MS.gene07991:intron |
| AAGATGATAGCAATTTCGAT+TGG | 0.575993 | 6.3:-32472442 | MS.gene07991:CDS |
| GGGATTGAATTCAACCAGGA+GGG | 0.608457 | 6.3:-32471966 | MS.gene07991:CDS |
| ATATAGAGCACAAACAAGAT+GGG | 0.613330 | 6.3:-32471986 | MS.gene07991:CDS |
| GGATTGAATTCAACCAGGAG+GGG | 0.622119 | 6.3:-32471965 | MS.gene07991:CDS |
| GAGATTGAAGGCATATCAGG+AGG | 0.648787 | 6.3:-32470686 | MS.gene07991:CDS |
| AGCATAATAGGTCTTTCCAA+AGG | 0.673220 | 6.3:+32471318 | None:intergenic |
| AGATGGGATTGAATTCAACC+AGG | 0.687557 | 6.3:-32471970 | MS.gene07991:CDS |
| TGGGATTGAATTCAACCAGG+AGG | 0.692590 | 6.3:-32471967 | MS.gene07991:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTAAAATTGACATATTAA+TGG | + | chr6.3:32471570-32471589 | None:intergenic | 10.0% |
| !! | AACATAATATATCAATTTGA+AGG | + | chr6.3:32471384-32471403 | None:intergenic | 15.0% |
| !!! | AAATCATGTGTATATTTTAT+AGG | - | chr6.3:32472189-32472208 | MS.gene07991:intron | 15.0% |
| !!! | ATATATTATGTTGACATTTT+TGG | - | chr6.3:32471392-32471411 | MS.gene07991:intron | 15.0% |
| !! | AAATGATTTAAGTAGGAAAT+AGG | + | chr6.3:32471886-32471905 | None:intergenic | 20.0% |
| !! | ATATGATGAAACTGAAAAAT+TGG | - | chr6.3:32472381-32472400 | MS.gene07991:intron | 20.0% |
| !! | TTAAAAATTGGTAAACAACA+AGG | - | chr6.3:32471646-32471665 | MS.gene07991:intron | 20.0% |
| !! | TTAGTAGCAAAAAAAAACAT+TGG | + | chr6.3:32471542-32471561 | None:intergenic | 20.0% |
| !! | TTCATCATATAATAAAAGCA+AGG | + | chr6.3:32472371-32472390 | None:intergenic | 20.0% |
| !!! | TATAAGTGTAAAAGTATGTT+GGG | - | chr6.3:32470810-32470829 | MS.gene07991:intron | 20.0% |
| !!! | TTGTTTACCAATTTTTAACA+TGG | + | chr6.3:32471644-32471663 | None:intergenic | 20.0% |
| !!! | TTTTCCATATCTTAACAAAT+TGG | - | chr6.3:32471504-32471523 | MS.gene07991:intron | 20.0% |
| ! | AACATCATCATTTACTTGAT+CGG | + | chr6.3:32471186-32471205 | None:intergenic | 25.0% |
| ! | AGTTTGATATTCACATAAAG+TGG | + | chr6.3:32471975-32471994 | None:intergenic | 25.0% |
| ! | ATACTTAATCAAAATGGGAT+TGG | + | chr6.3:32471041-32471060 | None:intergenic | 25.0% |
| ! | CTACACAATAGAACAAAATA+GGG | - | chr6.3:32472085-32472104 | MS.gene07991:intron | 25.0% |
| ! | GACACATACTTAATCAAAAT+GGG | + | chr6.3:32471046-32471065 | None:intergenic | 25.0% |
| ! | GAGTTTCAAATGATTTAAGT+AGG | + | chr6.3:32471893-32471912 | None:intergenic | 25.0% |
| ! | TACACAATAGAACAAAATAG+GGG | - | chr6.3:32472086-32472105 | MS.gene07991:intron | 25.0% |
| ! | TATTTACATGTTTCTCTAAC+AGG | - | chr6.3:32471612-32471631 | MS.gene07991:intron | 25.0% |
| ! | TCAATTATCTTGATCTGTTT+AGG | - | chr6.3:32471316-32471335 | MS.gene07991:CDS | 25.0% |
| ! | TGACACATACTTAATCAAAA+TGG | + | chr6.3:32471047-32471066 | None:intergenic | 25.0% |
| ! | TTATCAGTAAATGAGATTGA+AGG | - | chr6.3:32472420-32472439 | MS.gene07991:intron | 25.0% |
| ! | TTGCAAAACTATATTTCTCA+TGG | - | chr6.3:32472225-32472244 | MS.gene07991:intron | 25.0% |
| !! | AAAACACAACATAGATTTTC+AGG | + | chr6.3:32471297-32471316 | None:intergenic | 25.0% |
| !! | AATTATTTCTTCTTACTGCT+TGG | - | chr6.3:32471587-32471606 | MS.gene07991:intron | 25.0% |
| !! | ACAAACAAAGTATCATTGAA+TGG | + | chr6.3:32471949-32471968 | None:intergenic | 25.0% |
| !! | GTATAAGTGTAAAAGTATGT+TGG | - | chr6.3:32470809-32470828 | MS.gene07991:intron | 25.0% |
| !! | TCATGTTTTCATATTTCTCA+TGG | - | chr6.3:32472155-32472174 | MS.gene07991:intron | 25.0% |
| !!! | AAGCAAGGCTTTTTATTTTT+CGG | + | chr6.3:32472356-32472375 | None:intergenic | 25.0% |
| !!! | TCAATTCACAGATTCTTTTT+TGG | - | chr6.3:32470720-32470739 | MS.gene07991:intron | 25.0% |
| !!! | TTTTGATTAAGTATGTGTCA+TGG | - | chr6.3:32471047-32471066 | MS.gene07991:intron | 25.0% |
| AAACCAATTGTGATGAAGAA+GGG | + | chr6.3:32471348-32471367 | None:intergenic | 30.0% | |
| AATAGAAGCAGTGATCAATA+TGG | + | chr6.3:32470962-32470981 | None:intergenic | 30.0% | |
| ATATAGAGCACAAACAAGAT+GGG | - | chr6.3:32471132-32471151 | MS.gene07991:intron | 30.0% | |
| ATGTGTGAACAATTCAATCA+AGG | + | chr6.3:32471210-32471229 | None:intergenic | 30.0% | |
| CATGTGTAGTTCCAAAAATA+GGG | + | chr6.3:32471841-32471860 | None:intergenic | 30.0% | |
| CCTACACAATAGAACAAAAT+AGG | - | chr6.3:32472084-32472103 | MS.gene07991:intron | 30.0% | |
| GCATTTGAATCACATTTGAT+TGG | - | chr6.3:32470855-32470874 | MS.gene07991:intron | 30.0% | |
| GGGATGCATAGTATTTAATT+TGG | - | chr6.3:32470830-32470849 | MS.gene07991:intron | 30.0% | |
| GTGAATTGAACCTGAATTAT+CGG | + | chr6.3:32470709-32470728 | None:intergenic | 30.0% | |
| TAAACCAATTGTGATGAAGA+AGG | + | chr6.3:32471349-32471368 | None:intergenic | 30.0% | |
| TAGAGATTAAACATCTAACG+CGG | + | chr6.3:32471437-32471456 | None:intergenic | 30.0% | |
| TCACTTCAGTTGTGTTTATA+TGG | - | chr6.3:32471911-32471930 | MS.gene07991:intron | 30.0% | |
| TTTCAGATCGAATTCATCAT+TGG | - | chr6.3:32472272-32472291 | MS.gene07991:intron | 30.0% | |
| TTTGTTCTATTGTGTAGGTA+AGG | + | chr6.3:32472082-32472101 | None:intergenic | 30.0% | |
| ! | CCTATTTTGTTCTATTGTGT+AGG | + | chr6.3:32472087-32472106 | None:intergenic | 30.0% |
| !! | ACAAAAAGTACCTTATCAGT+TGG | + | chr6.3:32472314-32472333 | None:intergenic | 30.0% |
| !! | ATCGCCAATTTGTTAAGATA+TGG | + | chr6.3:32471511-32471530 | None:intergenic | 30.0% |
| !! | CAAAAAGTACCTTATCAGTT+GGG | + | chr6.3:32472313-32472332 | None:intergenic | 30.0% |
| AACCAATTGTGATGAAGAAG+GGG | + | chr6.3:32471347-32471366 | None:intergenic | 35.0% | |
| AGCATAATAGGTCTTTCCAA+AGG | + | chr6.3:32471803-32471822 | None:intergenic | 35.0% | |
| CCATGTGTAGTTCCAAAAAT+AGG | + | chr6.3:32471842-32471861 | None:intergenic | 35.0% | |
| GATATAGAGCACAAACAAGA+TGG | - | chr6.3:32471131-32471150 | MS.gene07991:intron | 35.0% | |
| GTTGCTGCCATGTTAAAAAT+TGG | - | chr6.3:32471634-32471653 | MS.gene07991:intron | 35.0% | |
| TCTGAAAAAGCAATCTGTTC+AGG | - | chr6.3:32471256-32471275 | MS.gene07991:intron | 35.0% | |
| TTTAGTGGTCAAGTGTTTAG+TGG | - | chr6.3:32471107-32471126 | MS.gene07991:intron | 35.0% | |
| ! | AAAAATAGGGTCTGAAGCTT+CGG | + | chr6.3:32471828-32471847 | None:intergenic | 35.0% |
| ! | AATGAGATTGAAGGCATATC+AGG | - | chr6.3:32472429-32472448 | MS.gene07991:CDS | 35.0% |
| ! | AGAGATGATCTGGAATTGTT+GGG | + | chr6.3:32471676-32471695 | None:intergenic | 35.0% |
| ! | ATACGAACGATGTTTTATGC+AGG | - | chr6.3:32472003-32472022 | MS.gene07991:CDS | 35.0% |
| ! | TTGGTGTTCTTTGTGTTTAG+TGG | - | chr6.3:32471092-32471111 | MS.gene07991:intron | 35.0% |
| !! | AACATAGATTTTCAGGAAGG+TGG | + | chr6.3:32471290-32471309 | None:intergenic | 35.0% |
| !! | CACAACATAGATTTTCAGGA+AGG | + | chr6.3:32471293-32471312 | None:intergenic | 35.0% |
| !! | CTGCATTTGTGTTTTTCAGT+TGG | - | chr6.3:32471073-32471092 | MS.gene07991:intron | 35.0% |
| !!! | CCTATTTTTGGAACTACACA+TGG | - | chr6.3:32471839-32471858 | MS.gene07991:intron | 35.0% |
| AAAACCCATCCCAACTGATA+AGG | - | chr6.3:32472301-32472320 | MS.gene07991:intron | 40.0% | |
| AAGTACCTTATCAGTTGGGA+TGG | + | chr6.3:32472309-32472328 | None:intergenic | 40.0% | |
| AGATGGGATTGAATTCAACC+AGG | - | chr6.3:32471148-32471167 | MS.gene07991:intron | 40.0% | |
| AGTACCTTATCAGTTGGGAT+GGG | + | chr6.3:32472308-32472327 | None:intergenic | 40.0% | |
| ATCTAACGCGGCAATTTCAA+AGG | + | chr6.3:32471425-32471444 | None:intergenic | 40.0% | |
| CCAATAATTCACTCGCTGAA+GGG | + | chr6.3:32471754-32471773 | None:intergenic | 40.0% | |
| CCAGATCATCTCTCAAATGA+AGG | - | chr6.3:32471683-32471702 | MS.gene07991:intron | 40.0% | |
| CCTTCATTTGAGAGATGATC+TGG | + | chr6.3:32471686-32471705 | None:intergenic | 40.0% | |
| GCTCATTGATGTATGAACTG+TGG | + | chr6.3:32470917-32470936 | None:intergenic | 40.0% | |
| TACACATGGAACAAAAGCTC+TGG | - | chr6.3:32471853-32471872 | MS.gene07991:intron | 40.0% | |
| TCCAATAATTCACTCGCTGA+AGG | + | chr6.3:32471755-32471774 | None:intergenic | 40.0% | |
| TGTGGCAAATGAGAAGTAAG+TGG | + | chr6.3:32470899-32470918 | None:intergenic | 40.0% | |
| TTGGATCACCCTGTTGTAAA+AGG | - | chr6.3:32471770-32471789 | MS.gene07991:intron | 40.0% | |
| ! | AAAATAGGGTCTGAAGCTTC+GGG | + | chr6.3:32471827-32471846 | None:intergenic | 40.0% |
| ! | CTGCTTCTATTTGCTACTTC+TGG | - | chr6.3:32470971-32470990 | MS.gene07991:intron | 40.0% |
| ! | GAAGCTTCAGACCCTATTTT+TGG | - | chr6.3:32471827-32471846 | MS.gene07991:intron | 40.0% |
| ! | GAGAGATGATCTGGAATTGT+TGG | + | chr6.3:32471677-32471696 | None:intergenic | 40.0% |
| ! | GTGTAGCACCTTTTACAACA+GGG | + | chr6.3:32471781-32471800 | None:intergenic | 40.0% |
| !! | TGTAAAAGGTGCTACACCTT+TGG | - | chr6.3:32471784-32471803 | MS.gene07991:intron | 40.0% |
| CCCTTCAGCGAGTGAATTAT+TGG | - | chr6.3:32471751-32471770 | MS.gene07991:intron | 45.0% | |
| GGATTGAATTCAACCAGGAG+GGG | - | chr6.3:32471153-32471172 | MS.gene07991:intron | 45.0% | |
| GGGATTGAATTCAACCAGGA+GGG | - | chr6.3:32471152-32471171 | MS.gene07991:intron | 45.0% | |
| TGCCCCTTCTTCATCACAAT+TGG | - | chr6.3:32471342-32471361 | MS.gene07991:CDS | 45.0% | |
| TGGGATTGAATTCAACCAGG+AGG | - | chr6.3:32471151-32471170 | MS.gene07991:intron | 45.0% | |
| TGGTGCACTTCCGATAATTC+AGG | - | chr6.3:32470696-32470715 | MS.gene07991:CDS | 45.0% | |
| ! | GAAGCTTCGGGAAGCATAAT+AGG | + | chr6.3:32471815-32471834 | None:intergenic | 45.0% |
| ! | GAGATTGAAGGCATATCAGG+AGG | - | chr6.3:32472432-32472451 | MS.gene07991:CDS | 45.0% |
| ! | GGTGTAGCACCTTTTACAAC+AGG | + | chr6.3:32471782-32471801 | None:intergenic | 45.0% |
| CTCGCTGAAGGGCGAACACG+TGG | + | chr6.3:32471743-32471762 | None:intergenic | 65.0% | |
| GATCGGCATCTGTCCCCTCC+TGG | + | chr6.3:32471169-32471188 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 32470679 | 32472461 | 32470679 | ID=MS.gene07991 |
| chr6.3 | mRNA | 32470679 | 32472461 | 32470679 | ID=MS.gene07991.t1;Parent=MS.gene07991 |
| chr6.3 | exon | 32472423 | 32472461 | 32472423 | ID=MS.gene07991.t1.exon1;Parent=MS.gene07991.t1 |
| chr6.3 | CDS | 32472423 | 32472461 | 32472423 | ID=cds.MS.gene07991.t1;Parent=MS.gene07991.t1 |
| chr6.3 | exon | 32471950 | 32472048 | 32471950 | ID=MS.gene07991.t1.exon2;Parent=MS.gene07991.t1 |
| chr6.3 | CDS | 32471950 | 32472048 | 32471950 | ID=cds.MS.gene07991.t1;Parent=MS.gene07991.t1 |
| chr6.3 | exon | 32471485 | 32471506 | 32471485 | ID=MS.gene07991.t1.exon3;Parent=MS.gene07991.t1 |
| chr6.3 | CDS | 32471485 | 32471506 | 32471485 | ID=cds.MS.gene07991.t1;Parent=MS.gene07991.t1 |
| chr6.3 | exon | 32471266 | 32471381 | 32471266 | ID=MS.gene07991.t1.exon4;Parent=MS.gene07991.t1 |
| chr6.3 | CDS | 32471266 | 32471381 | 32471266 | ID=cds.MS.gene07991.t1;Parent=MS.gene07991.t1 |
| chr6.3 | exon | 32470679 | 32470720 | 32470679 | ID=MS.gene07991.t1.exon5;Parent=MS.gene07991.t1 |
| chr6.3 | CDS | 32470679 | 32470720 | 32470679 | ID=cds.MS.gene07991.t1;Parent=MS.gene07991.t1 |
| Gene Sequence |
| Protein sequence |
