Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08112.t1 | XP_013460118.1 | 96.3 | 164 | 6 | 0 | 2 | 165 | 3 | 166 | 3.00E-83 | 317.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08112.t1 | B7FGG7 | 96.3 | 164 | 6 | 0 | 2 | 165 | 3 | 166 | 2.2e-83 | 317.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052309 | MS.gene08112 | -0.812151 | 4.90E-51 | -1.69E-46 |
| MS.gene058201 | MS.gene08112 | 0.801879 | 7.31E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08112.t1 | MTR_3g462220 | 96.341 | 164 | 6 | 0 | 2 | 165 | 3 | 166 | 3.54e-117 | 328 |
| MS.gene08112.t1 | MTR_5g016450 | 38.372 | 172 | 91 | 6 | 5 | 165 | 10 | 177 | 6.40e-27 | 100 |
| MS.gene08112.t1 | MTR_5g016450 | 38.596 | 171 | 91 | 6 | 5 | 165 | 10 | 176 | 1.02e-26 | 99.8 |
| MS.gene08112.t1 | MTR_5g016450 | 38.816 | 152 | 80 | 5 | 5 | 147 | 10 | 157 | 1.89e-25 | 95.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08112.t1 | AT4G37300 | 39.205 | 176 | 92 | 6 | 1 | 165 | 1 | 172 | 2.53e-26 | 98.6 |
Find 24 sgRNAs with CRISPR-Local
Find 94 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTTGAAATTGTAGCATTTC+AGG | 0.143934 | 3.3:-49929157 | None:intergenic |
| TTGGTGCCACTTCTTCAAAA+TGG | 0.336944 | 3.3:-49929060 | None:intergenic |
| CTTTCACCACAGACAGGAAA+AGG | 0.363878 | 3.3:+49930640 | MS.gene08112:intron |
| TTTCAGTTATCACTCCTGTT+AGG | 0.372318 | 3.3:-49930909 | None:intergenic |
| GAGTCATGAGTGTCGTTATC+TGG | 0.392302 | 3.3:-49930706 | None:intergenic |
| GGAACCCAGTCATTGGTTGC+TGG | 0.416375 | 3.3:-49930751 | None:intergenic |
| AGATGAGTGTCACTTCTGTT+TGG | 0.417853 | 3.3:-49928995 | None:intergenic |
| TGAAGTAGCTGAAGAATTTG+TGG | 0.418411 | 3.3:+49929829 | MS.gene08112:CDS |
| ACAATTCAGACAAACCTAAC+AGG | 0.431560 | 3.3:+49930895 | MS.gene08112:CDS |
| GCAATCCAGCAACCAATGAC+TGG | 0.433217 | 3.3:+49930746 | MS.gene08112:CDS |
| TGACTCTCATCACTCTACTA+AGG | 0.462846 | 3.3:+49930723 | MS.gene08112:CDS |
| AGGAGCTGGAACCCAGTCAT+TGG | 0.486673 | 3.3:-49930758 | None:intergenic |
| GTTCAGAAATTGGTTTACGA+TGG | 0.533628 | 3.3:+49929803 | MS.gene08112:intron |
| AATACCTCACTGAAAGGAGC+TGG | 0.537040 | 3.3:-49930772 | None:intergenic |
| GACTCTCATCACTCTACTAA+GGG | 0.542573 | 3.3:+49930724 | MS.gene08112:CDS |
| TTTCACCACAGACAGGAAAA+GGG | 0.552205 | 3.3:+49930641 | MS.gene08112:intron |
| TGAAAATGATCCTCAAGAGA+AGG | 0.574785 | 3.3:+49929183 | MS.gene08112:CDS |
| TAGGAACAAACCTTCTCTTG+AGG | 0.587802 | 3.3:-49929193 | None:intergenic |
| CAATCCAGCAACCAATGACT+GGG | 0.590043 | 3.3:+49930747 | MS.gene08112:CDS |
| ATGTAAAATACCTCACTGAA+AGG | 0.593015 | 3.3:-49930778 | None:intergenic |
| GGTTCCAGCTCCTTTCAGTG+AGG | 0.664768 | 3.3:+49930768 | MS.gene08112:CDS |
| TGTCGTTATCTGGTTCTATG+TGG | 0.665597 | 3.3:-49930696 | None:intergenic |
| CGTAGACAAACATCATCACA+CGG | 0.672277 | 3.3:+49929880 | MS.gene08112:CDS |
| GAAGCATACTCACTACGTTG+AGG | 0.680375 | 3.3:-49929097 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAGATTTATTAACTAAAA+TGG | - | chr3.3:49930457-49930476 | None:intergenic | 10.0% |
| !! | TAGAAATTAGAAAATAAAAT+AGG | - | chr3.3:49930041-49930060 | None:intergenic | 10.0% |
| !! | TCTATTTAATATATTAACTT+AGG | + | chr3.3:49929428-49929447 | MS.gene08112:intron | 10.0% |
| !!! | AATTTTTTTTTTTGAAACAA+CGG | + | chr3.3:49929504-49929523 | MS.gene08112:intron | 10.0% |
| !!! | ATAAAGTTTTTTTTTTTTGA+AGG | - | chr3.3:49930091-49930110 | None:intergenic | 10.0% |
| !!! | TATTAATATAATGGATAAAA+GGG | - | chr3.3:49930431-49930450 | None:intergenic | 10.0% |
| !!! | TTATTAATATAATGGATAAA+AGG | - | chr3.3:49930432-49930451 | None:intergenic | 10.0% |
| !!! | TTTTATTTTCTAATTTCTAA+TGG | + | chr3.3:49930042-49930061 | MS.gene08112:intron | 10.0% |
| !!! | AAATGGTCTTATTAATATAA+TGG | - | chr3.3:49930440-49930459 | None:intergenic | 15.0% |
| !!! | TATTTAACTTATTATGAAAC+TGG | - | chr3.3:49929260-49929279 | None:intergenic | 15.0% |
| !! | AAAAATAATACTAGTCAAGT+AGG | - | chr3.3:49929920-49929939 | None:intergenic | 20.0% |
| !! | AATGAAAAAGGGAAAAAAAA+AGG | - | chr3.3:49929773-49929792 | None:intergenic | 20.0% |
| !! | AGAAAGATACAAAAAGTTAA+TGG | + | chr3.3:49930226-49930245 | MS.gene08112:intron | 20.0% |
| !! | AGACAATTATATCAACAATT+TGG | - | chr3.3:49929664-49929683 | None:intergenic | 20.0% |
| !! | ATCTATTAAATATGATTCGT+TGG | - | chr3.3:49930126-49930145 | None:intergenic | 20.0% |
| !! | ATGTTGACCAAAAAAATTAA+AGG | + | chr3.3:49929542-49929561 | MS.gene08112:intron | 20.0% |
| !! | ATTGAGAAAATAATAGCATT+TGG | + | chr3.3:49930584-49930603 | MS.gene08112:intron | 20.0% |
| !! | TATAATTGTCTCTTGTAAAT+AGG | + | chr3.3:49929673-49929692 | MS.gene08112:intron | 20.0% |
| !!! | ATCATCACCTTTAATTTTTT+TGG | - | chr3.3:49929552-49929571 | None:intergenic | 20.0% |
| !!! | ATTTTCTAATTTCTAATGGA+GGG | + | chr3.3:49930046-49930065 | MS.gene08112:intron | 20.0% |
| !!! | CTCATATTAAATGTTTTCTA+AGG | - | chr3.3:49930529-49930548 | None:intergenic | 20.0% |
| !!! | CTTTGATTTGAATATGTTTT+AGG | + | chr3.3:49930866-49930885 | MS.gene08112:intron | 20.0% |
| !!! | TATTTTCTAATTTCTAATGG+AGG | + | chr3.3:49930045-49930064 | MS.gene08112:intron | 20.0% |
| ! | ACATAATTGTGGAATGAAAA+AGG | - | chr3.3:49929785-49929804 | None:intergenic | 25.0% |
| ! | CATAATTGTGGAATGAAAAA+GGG | - | chr3.3:49929784-49929803 | None:intergenic | 25.0% |
| ! | TTAGAATTATTGTCTAGAGA+TGG | + | chr3.3:49930366-49930385 | MS.gene08112:intron | 25.0% |
| !! | GAGATAATTGACTTACAAAT+AGG | - | chr3.3:49929615-49929634 | None:intergenic | 25.0% |
| !!! | AATATAATGGATAAAAGGGA+CGG | - | chr3.3:49930427-49930446 | None:intergenic | 25.0% |
| !!! | TGATTTGAATATGTTTTAGG+AGG | + | chr3.3:49930869-49930888 | MS.gene08112:intron | 25.0% |
| AGTTAATGGTCATCAAAGAT+AGG | + | chr3.3:49930240-49930259 | MS.gene08112:intron | 30.0% | |
| ATGTAAAATACCTCACTGAA+AGG | - | chr3.3:49930781-49930800 | None:intergenic | 30.0% | |
| CAACAAAGTATGTACATGAA+TGG | - | chr3.3:49929972-49929991 | None:intergenic | 30.0% | |
| CCAATTTCTGAACATAATTG+TGG | - | chr3.3:49929796-49929815 | None:intergenic | 30.0% | |
| CCACAATTATGTTCAGAAAT+TGG | + | chr3.3:49929793-49929812 | MS.gene08112:intron | 30.0% | |
| CGTGGAAATAAAACTTTAAG+AGG | - | chr3.3:49929234-49929253 | None:intergenic | 30.0% | |
| GTTAATGGTCATCAAAGATA+GGG | + | chr3.3:49930241-49930260 | MS.gene08112:intron | 30.0% | |
| TAAATGCTAGATGCAAATCT+AGG | - | chr3.3:49930151-49930170 | None:intergenic | 30.0% | |
| TAATACTAGTCAAGTAGGAA+TGG | - | chr3.3:49929915-49929934 | None:intergenic | 30.0% | |
| TTATTATGAAACTGGAAACG+TGG | - | chr3.3:49929252-49929271 | None:intergenic | 30.0% | |
| ! | GAGGAAAAACATTGTGTTTT+AGG | - | chr3.3:49929215-49929234 | None:intergenic | 30.0% |
| !! | ATATAATGGATAAAAGGGAC+GGG | - | chr3.3:49930426-49930445 | None:intergenic | 30.0% |
| ACAATTCAGACAAACCTAAC+AGG | + | chr3.3:49930895-49930914 | MS.gene08112:CDS | 35.0% | |
| ACTACTAGTAGTAATGCCTT+TGG | + | chr3.3:49929588-49929607 | MS.gene08112:intron | 35.0% | |
| CGTTGAAATTGTAGCATTTC+AGG | - | chr3.3:49929160-49929179 | None:intergenic | 35.0% | |
| TGAAAATGATCCTCAAGAGA+AGG | + | chr3.3:49929183-49929202 | MS.gene08112:CDS | 35.0% | |
| TGAAGTAGCTGAAGAATTTG+TGG | + | chr3.3:49929829-49929848 | MS.gene08112:CDS | 35.0% | |
| TGACTTACAAATAGGACCAA+AGG | - | chr3.3:49929607-49929626 | None:intergenic | 35.0% | |
| ! | CTAGCATTTACACATCGATT+TGG | + | chr3.3:49930161-49930180 | MS.gene08112:intron | 35.0% |
| ! | GTTCAGAAATTGGTTTACGA+TGG | + | chr3.3:49929803-49929822 | MS.gene08112:intron | 35.0% |
| ! | TAGTATGATTTTGCGTTTGC+CGG | + | chr3.3:49929323-49929342 | MS.gene08112:intron | 35.0% |
| !! | AAGACACCATTTTGAAGAAG+TGG | + | chr3.3:49929054-49929073 | MS.gene08112:CDS | 35.0% |
| !!! | GTGTCTTGTTTTTGCTTCTA+TGG | - | chr3.3:49929041-49929060 | None:intergenic | 35.0% |
| ATCAAGCGTTTGTAGTCCAA+CGG | - | chr3.3:49929391-49929410 | None:intergenic | 40.0% | |
| CGTAGACAAACATCATCACA+CGG | + | chr3.3:49929880-49929899 | MS.gene08112:CDS | 40.0% | |
| CTAGAGATGGTAAAGCTAGT+TGG | + | chr3.3:49930379-49930398 | MS.gene08112:intron | 40.0% | |
| GACTCTCATCACTCTACTAA+GGG | + | chr3.3:49930724-49930743 | MS.gene08112:CDS | 40.0% | |
| TAGGAACAAACCTTCTCTTG+AGG | - | chr3.3:49929196-49929215 | None:intergenic | 40.0% | |
| TGACTCTCATCACTCTACTA+AGG | + | chr3.3:49930723-49930742 | MS.gene08112:CDS | 40.0% | |
| TGTTTACTTTCACCACAGAC+AGG | + | chr3.3:49930634-49930653 | MS.gene08112:intron | 40.0% | |
| TTTCACCACAGACAGGAAAA+GGG | + | chr3.3:49930641-49930660 | MS.gene08112:intron | 40.0% | |
| ! | AGTATGATTTTGCGTTTGCC+GGG | + | chr3.3:49929324-49929343 | MS.gene08112:intron | 40.0% |
| ! | ATAAACCCTTTTCCTGTCTG+TGG | - | chr3.3:49930649-49930668 | None:intergenic | 40.0% |
| !! | AGATGAGTGTCACTTCTGTT+TGG | - | chr3.3:49928998-49929017 | None:intergenic | 40.0% |
| !! | TGAGGCTTTGTGTGTCTTTT+TGG | - | chr3.3:49929082-49929101 | None:intergenic | 40.0% |
| !! | TGTCGTTATCTGGTTCTATG+TGG | - | chr3.3:49930699-49930718 | None:intergenic | 40.0% |
| !! | TTGGTGCCACTTCTTCAAAA+TGG | - | chr3.3:49929063-49929082 | None:intergenic | 40.0% |
| AAGGCAATTATCCTGACCGT+TGG | + | chr3.3:49929372-49929391 | MS.gene08112:intron | 45.0% | |
| AATACCTCACTGAAAGGAGC+TGG | - | chr3.3:49930775-49930794 | None:intergenic | 45.0% | |
| ATGGTCATCAAAGATAGGGC+TGG | + | chr3.3:49930245-49930264 | MS.gene08112:intron | 45.0% | |
| CAATCCAGCAACCAATGACT+GGG | + | chr3.3:49930747-49930766 | MS.gene08112:CDS | 45.0% | |
| CTTTCACCACAGACAGGAAA+AGG | + | chr3.3:49930640-49930659 | MS.gene08112:intron | 45.0% | |
| GAAGCATACTCACTACGTTG+AGG | - | chr3.3:49929100-49929119 | None:intergenic | 45.0% | |
| TGGTAAAGCTAGTTGGCACT+TGG | + | chr3.3:49930386-49930405 | MS.gene08112:intron | 45.0% | |
| ! | GAGTCATGAGTGTCGTTATC+TGG | - | chr3.3:49930709-49930728 | None:intergenic | 45.0% |
| CAAAGATAGGGCTGGATCGA+GGG | + | chr3.3:49930253-49930272 | MS.gene08112:intron | 50.0% | |
| GCAATCCAGCAACCAATGAC+TGG | + | chr3.3:49930746-49930765 | MS.gene08112:CDS | 50.0% | |
| TCAAAGATAGGGCTGGATCG+AGG | + | chr3.3:49930252-49930271 | MS.gene08112:intron | 50.0% | |
| TGCCTTCCAAGCAATAGACC+CGG | - | chr3.3:49929358-49929377 | None:intergenic | 50.0% | |
| ACCCGGGTCTATTGCTTGGA+AGG | + | chr3.3:49929353-49929372 | MS.gene08112:intron | 55.0% | |
| AGGAGCTGGAACCCAGTCAT+TGG | - | chr3.3:49930761-49930780 | None:intergenic | 55.0% | |
| GCCTTCCAAGCAATAGACCC+GGG | - | chr3.3:49929357-49929376 | None:intergenic | 55.0% | |
| GCGTTTGTAGTCCAACGGTC+AGG | - | chr3.3:49929386-49929405 | None:intergenic | 55.0% | |
| GGTTCCAGCTCCTTTCAGTG+AGG | + | chr3.3:49930768-49930787 | MS.gene08112:CDS | 55.0% | |
| TCGAACCCGGGTCTATTGCT+TGG | + | chr3.3:49929349-49929368 | MS.gene08112:intron | 55.0% | |
| ! | GGAACCCAGTCATTGGTTGC+TGG | - | chr3.3:49930754-49930773 | None:intergenic | 55.0% |
| AAAAGGGACGGGCCCAAGTG+TGG | - | chr3.3:49930415-49930434 | None:intergenic | 60.0% | |
| ATAGACCCGGGTTCGACTCC+CGG | - | chr3.3:49929345-49929364 | None:intergenic | 60.0% | |
| CGAGGGTCTATCGGTGTGTC+TGG | + | chr3.3:49930270-49930289 | MS.gene08112:intron | 60.0% | |
| GGGCTGGATCGAGGGTCTAT+CGG | + | chr3.3:49930261-49930280 | MS.gene08112:intron | 60.0% | |
| CGTTTGCCGGGAGTCGAACC+CGG | + | chr3.3:49929336-49929355 | MS.gene08112:intron | 65.0% | |
| GGCACTTGGCCGCCACACTT+GGG | + | chr3.3:49930400-49930419 | MS.gene08112:intron | 65.0% | |
| GTTTGCCGGGAGTCGAACCC+GGG | + | chr3.3:49929337-49929356 | MS.gene08112:intron | 65.0% | |
| TGGCACTTGGCCGCCACACT+TGG | + | chr3.3:49930399-49930418 | MS.gene08112:intron | 65.0% | |
| AGGGACGGGCCCAAGTGTGG+CGG | - | chr3.3:49930412-49930431 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 49928980 | 49930929 | 49928980 | ID=MS.gene08112 |
| chr3.3 | mRNA | 49928980 | 49930929 | 49928980 | ID=MS.gene08112.t1;Parent=MS.gene08112 |
| chr3.3 | exon | 49928980 | 49929204 | 49928980 | ID=MS.gene08112.t1.exon1;Parent=MS.gene08112.t1 |
| chr3.3 | CDS | 49928980 | 49929204 | 49928980 | ID=cds.MS.gene08112.t1;Parent=MS.gene08112.t1 |
| chr3.3 | exon | 49929809 | 49929901 | 49929809 | ID=MS.gene08112.t1.exon2;Parent=MS.gene08112.t1 |
| chr3.3 | CDS | 49929809 | 49929901 | 49929809 | ID=cds.MS.gene08112.t1;Parent=MS.gene08112.t1 |
| chr3.3 | exon | 49930652 | 49930789 | 49930652 | ID=MS.gene08112.t1.exon3;Parent=MS.gene08112.t1 |
| chr3.3 | CDS | 49930652 | 49930789 | 49930652 | ID=cds.MS.gene08112.t1;Parent=MS.gene08112.t1 |
| chr3.3 | exon | 49930888 | 49930929 | 49930888 | ID=MS.gene08112.t1.exon4;Parent=MS.gene08112.t1 |
| chr3.3 | CDS | 49930888 | 49930929 | 49930888 | ID=cds.MS.gene08112.t1;Parent=MS.gene08112.t1 |
| Gene Sequence |
| Protein sequence |
