Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08180.t1 | XP_003624662.1 | 90.5 | 168 | 15 | 1 | 25 | 191 | 35 | 202 | 8.60E-74 | 286.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08180.t1 | G7L0H2 | 90.5 | 168 | 15 | 1 | 25 | 191 | 35 | 202 | 6.2e-74 | 286.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050807 | MS.gene08180 | 0.803256 | 3.80E-49 | -1.69E-46 |
| MS.gene051719 | MS.gene08180 | 0.819231 | 1.29E-52 | -1.69E-46 |
| MS.gene051720 | MS.gene08180 | 0.815492 | 8.99E-52 | -1.69E-46 |
| MS.gene052516 | MS.gene08180 | 0.80773 | 4.38E-50 | -1.69E-46 |
| MS.gene055144 | MS.gene08180 | 0.824855 | 6.44E-54 | -1.69E-46 |
| MS.gene055465 | MS.gene08180 | 0.809608 | 1.74E-50 | -1.69E-46 |
| MS.gene055466 | MS.gene08180 | 0.812295 | 4.55E-51 | -1.69E-46 |
| MS.gene058339 | MS.gene08180 | 0.838407 | 2.93E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08180.t1 | MTR_7g086130 | 89.286 | 168 | 17 | 1 | 25 | 191 | 35 | 202 | 1.53e-87 | 256 |
| MS.gene08180.t1 | MTR_7g086130 | 85.475 | 179 | 24 | 2 | 15 | 191 | 29 | 207 | 4.55e-87 | 255 |
| MS.gene08180.t1 | MTR_6g478190 | 43.750 | 64 | 36 | 0 | 24 | 87 | 37 | 100 | 1.92e-13 | 65.5 |
| MS.gene08180.t1 | MTR_5g087450 | 41.270 | 63 | 37 | 0 | 25 | 87 | 38 | 100 | 7.58e-12 | 62.8 |
| MS.gene08180.t1 | MTR_5g087470 | 37.634 | 93 | 49 | 3 | 25 | 108 | 37 | 129 | 8.79e-12 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08180.t1 | AT1G54400 | 41.791 | 67 | 39 | 0 | 25 | 91 | 38 | 104 | 1.23e-11 | 60.8 |
Find 31 sgRNAs with CRISPR-Local
Find 77 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCATCTTCATTGCAATATTT+TGG | 0.150093 | 7.3:+22691040 | None:intergenic |
| TCTGGCATTTGTTCTTCTTC+TGG | 0.224256 | 7.3:+22690941 | None:intergenic |
| ACATATCAGAGTTTGGAATT+TGG | 0.240193 | 7.3:-22690884 | MS.gene08180:CDS |
| TACACAACAGACAACAAGTT+TGG | 0.336570 | 7.3:-22690815 | MS.gene08180:CDS |
| AGAGGTTGCTCTTAAATTCA+TGG | 0.354880 | 7.3:-22690768 | MS.gene08180:CDS |
| GTCCATTTGATGGAACTAAA+TGG | 0.359566 | 7.3:-22691090 | MS.gene08180:CDS |
| ATATCCTCATCTTCAAGTTC+TGG | 0.365913 | 7.3:+22690923 | None:intergenic |
| CGAATTCAAATCAACCATCT+TGG | 0.372715 | 7.3:-22691136 | MS.gene08180:CDS |
| TTCCATTTAGTTCCATCAAA+TGG | 0.385038 | 7.3:+22691088 | None:intergenic |
| TTGTTTACCTTTCCAAGTCT+TGG | 0.397452 | 7.3:+22691803 | None:intergenic |
| AGTGGAGAGCGTCCATTTGA+TGG | 0.411520 | 7.3:-22691100 | MS.gene08180:CDS |
| GAATTCAAATCAACCATCTT+GGG | 0.419246 | 7.3:-22691135 | MS.gene08180:CDS |
| CCCTCGAGCTGGCCAAGACT+TGG | 0.443777 | 7.3:-22691815 | MS.gene08180:CDS |
| GGTTGTGATAATTGTTGTAA+TGG | 0.450646 | 7.3:-22690747 | MS.gene08180:CDS |
| CCAAGTCTTGGCCAGCTCGA+GGG | 0.483198 | 7.3:+22691815 | None:intergenic |
| GGTTATTGTAAATTATCTTG+TGG | 0.496692 | 7.3:-22690726 | MS.gene08180:CDS |
| TGCAATGAAGATGCCATTCG+TGG | 0.501501 | 7.3:-22691031 | MS.gene08180:CDS |
| TCCAAGTCTTGGCCAGCTCG+AGG | 0.506208 | 7.3:+22691814 | None:intergenic |
| TCTTCTTCTGGTTCCTCCTG+AGG | 0.515123 | 7.3:+22690953 | None:intergenic |
| TATCAGAGTTTGGAATTTGG+AGG | 0.521412 | 7.3:-22690881 | MS.gene08180:CDS |
| TTACTATATACCCCTCGAGC+TGG | 0.530421 | 7.3:-22691826 | MS.gene08180:CDS |
| AAAAGTTGATCTGATACCTC+AGG | 0.531531 | 7.3:-22690969 | MS.gene08180:CDS |
| CAAGTCTTGGCCAGCTCGAG+GGG | 0.541674 | 7.3:+22691816 | None:intergenic |
| TTGCATGAAATTGCCACGAA+TGG | 0.562966 | 7.3:+22691018 | None:intergenic |
| AGCAAAACCGTCATGGCTCA+AGG | 0.573165 | 7.3:-22690698 | MS.gene08180:CDS |
| AATGCCAGAACTTGAAGATG+AGG | 0.591611 | 7.3:-22690927 | MS.gene08180:CDS |
| GAGCTGGCCAAGACTTGGAA+AGG | 0.596092 | 7.3:-22691810 | MS.gene08180:intron |
| TGATTGACCTTGAGCCATGA+CGG | 0.602155 | 7.3:+22690691 | None:intergenic |
| GGATATGAGCAAAACCGTCA+TGG | 0.621622 | 7.3:-22690705 | MS.gene08180:CDS |
| TGATGTAGAAACTACTAGAG+AGG | 0.643103 | 7.3:-22690786 | MS.gene08180:CDS |
| AGTTGATCTGATACCTCAGG+AGG | 0.662755 | 7.3:-22690966 | MS.gene08180:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAATTTGTAACTTTATA+AGG | + | chr7.3:22691187-22691206 | None:intergenic | 10.0% |
| !!! | AACATTTTACTAATTTTATA+AGG | + | chr7.3:22691125-22691144 | None:intergenic | 10.0% |
| !!! | ATAAAGTTACAAATTTTTTT+TGG | - | chr7.3:22691188-22691207 | MS.gene08180:intron | 10.0% |
| !! | AAATGAAATTGAATTAAACA+AGG | + | chr7.3:22691030-22691049 | None:intergenic | 15.0% |
| !! | TCATGTATCTAAATTATATA+TGG | - | chr7.3:22691003-22691022 | MS.gene08180:CDS | 15.0% |
| !! | TGATGATCAATAAATTTATT+AGG | + | chr7.3:22690841-22690860 | None:intergenic | 15.0% |
| !!! | AAAAATTAGCAAATCATTTT+TGG | + | chr7.3:22691266-22691285 | None:intergenic | 15.0% |
| !!! | ACATTGTTTTTGTTAAATTT+AGG | + | chr7.3:22691292-22691311 | None:intergenic | 15.0% |
| !!! | ATTAGTGTTTTTAAAAGTTT+GGG | - | chr7.3:22691145-22691164 | MS.gene08180:CDS | 15.0% |
| !!! | CATTGTTTTTGTTAAATTTA+GGG | + | chr7.3:22691291-22691310 | None:intergenic | 15.0% |
| !!! | TATTAGTGTTTTTAAAAGTT+TGG | - | chr7.3:22691144-22691163 | MS.gene08180:CDS | 15.0% |
| !!! | TTTTACTAATTTTATAAGGA+TGG | + | chr7.3:22691121-22691140 | None:intergenic | 15.0% |
| !! | CAAAGATTTGTGAAATAAAA+TGG | - | chr7.3:22690870-22690889 | MS.gene08180:CDS | 20.0% |
| !!! | CAATAAATTTATTAGGTTGA+AGG | + | chr7.3:22690834-22690853 | None:intergenic | 20.0% |
| !!! | CATTTTATTTCACAAATCTT+TGG | + | chr7.3:22690872-22690891 | None:intergenic | 20.0% |
| !!! | TTAGTGTTTTTAAAAGTTTG+GGG | - | chr7.3:22691146-22691165 | MS.gene08180:CDS | 20.0% |
| ! | GAAAAATACATATCAGAGTT+TGG | - | chr7.3:22691646-22691665 | MS.gene08180:intron | 25.0% |
| ! | GGTTATTGTAAATTATCTTG+TGG | - | chr7.3:22691811-22691830 | MS.gene08180:CDS | 25.0% |
| ! | TTGGCTTAAATACAGTAAAA+AGG | + | chr7.3:22691247-22691266 | None:intergenic | 25.0% |
| !!! | ATTTTGATCCTGTTTTTAAG+TGG | - | chr7.3:22690926-22690945 | MS.gene08180:CDS | 25.0% |
| !!! | GTTTGGGGTAAATATAATTT+TGG | - | chr7.3:22691161-22691180 | MS.gene08180:intron | 25.0% |
| GAATTCAAATCAACCATCTT+GGG | - | chr7.3:22691402-22691421 | MS.gene08180:intron | 30.0% | |
| GGAATTAAACCTACTAATGA+AGG | + | chr7.3:22690813-22690832 | None:intergenic | 30.0% | |
| GGTTGTGATAATTGTTGTAA+TGG | - | chr7.3:22691790-22691809 | MS.gene08180:intron | 30.0% | |
| TTCAAATTTGCCTTAAGAGT+TGG | + | chr7.3:22690780-22690799 | None:intergenic | 30.0% | |
| ! | ACATATCAGAGTTTGGAATT+TGG | - | chr7.3:22691653-22691672 | MS.gene08180:intron | 30.0% |
| ! | CTTTATTTGAGAGTTATGAG+GGG | - | chr7.3:22691340-22691359 | MS.gene08180:intron | 30.0% |
| ! | GCATCTTCATTGCAATATTT+TGG | + | chr7.3:22691500-22691519 | None:intergenic | 30.0% |
| ! | TTCCATTTAGTTCCATCAAA+TGG | + | chr7.3:22691452-22691471 | None:intergenic | 30.0% |
| !! | GCTTTATTTGAGAGTTATGA+GGG | - | chr7.3:22691339-22691358 | MS.gene08180:intron | 30.0% |
| !!! | ATAGACTTTTTTCACAGACT+AGG | - | chr7.3:22691316-22691335 | MS.gene08180:intron | 30.0% |
| !!! | GGGGTTATTAATTTGTTTTC+AGG | - | chr7.3:22691359-22691378 | MS.gene08180:intron | 30.0% |
| !!! | GGTATCAGATCAACTTTTTT+TGG | + | chr7.3:22691566-22691585 | None:intergenic | 30.0% |
| !!! | TAGACTTTTTTCACAGACTA+GGG | - | chr7.3:22691317-22691336 | MS.gene08180:intron | 30.0% |
| AAAGCAAACATGCACATAAC+CGG | - | chr7.3:22690893-22690912 | MS.gene08180:CDS | 35.0% | |
| AAGACTTGGAAAGGTAAACA+AGG | - | chr7.3:22690736-22690755 | MS.gene08180:CDS | 35.0% | |
| AGAGGTTGCTCTTAAATTCA+TGG | - | chr7.3:22691769-22691788 | MS.gene08180:intron | 35.0% | |
| ATACATGAAATTCACATGCC+TGG | + | chr7.3:22690991-22691010 | None:intergenic | 35.0% | |
| CGAATTCAAATCAACCATCT+TGG | - | chr7.3:22691401-22691420 | MS.gene08180:intron | 35.0% | |
| GATCAAAATCCTCATAAGAC+CGG | + | chr7.3:22690915-22690934 | None:intergenic | 35.0% | |
| GTCCATTTGATGGAACTAAA+TGG | - | chr7.3:22691447-22691466 | MS.gene08180:intron | 35.0% | |
| GTGTATACTCGTGTTAACAA+AGG | - | chr7.3:22691063-22691082 | MS.gene08180:CDS | 35.0% | |
| TACACAACAGACAACAAGTT+TGG | - | chr7.3:22691722-22691741 | MS.gene08180:intron | 35.0% | |
| TAGCTCATAACCAACTCTTA+AGG | - | chr7.3:22690767-22690786 | MS.gene08180:CDS | 35.0% | |
| TGATGTAGAAACTACTAGAG+AGG | - | chr7.3:22691751-22691770 | MS.gene08180:intron | 35.0% | |
| TTATCACCAAAAACCCAAGA+TGG | + | chr7.3:22691418-22691437 | None:intergenic | 35.0% | |
| TTGTTTACCTTTCCAAGTCT+TGG | + | chr7.3:22690737-22690756 | None:intergenic | 35.0% | |
| ! | AAAAGTTGATCTGATACCTC+AGG | - | chr7.3:22691568-22691587 | MS.gene08180:intron | 35.0% |
| ! | AATCAACCATCTTGGGTTTT+TGG | - | chr7.3:22691409-22691428 | MS.gene08180:intron | 35.0% |
| ! | ATATCCTCATCTTCAAGTTC+TGG | + | chr7.3:22691617-22691636 | None:intergenic | 35.0% |
| ! | TATCAGAGTTTGGAATTTGG+AGG | - | chr7.3:22691656-22691675 | MS.gene08180:intron | 35.0% |
| !! | GGCTTTATTTGAGAGTTATG+AGG | - | chr7.3:22691338-22691357 | MS.gene08180:intron | 35.0% |
| AATGCCAGAACTTGAAGATG+AGG | - | chr7.3:22691610-22691629 | MS.gene08180:intron | 40.0% | |
| TCTCTACGCCACTTAAAAAC+AGG | + | chr7.3:22690937-22690956 | None:intergenic | 40.0% | |
| TCTGGCATTTGTTCTTCTTC+TGG | + | chr7.3:22691599-22691618 | None:intergenic | 40.0% | |
| TTGCATGAAATTGCCACGAA+TGG | + | chr7.3:22691522-22691541 | None:intergenic | 40.0% | |
| ! | AACCTACTAATGAAGGCACA+AGG | + | chr7.3:22690806-22690825 | None:intergenic | 40.0% |
| ! | ACCTACTAATGAAGGCACAA+GGG | + | chr7.3:22690805-22690824 | None:intergenic | 40.0% |
| !!! | CTTGGGTTTTTGGTGATAAG+TGG | - | chr7.3:22691419-22691438 | MS.gene08180:intron | 40.0% |
| ACCATTGAGCTTCATCTTCC+AGG | - | chr7.3:22690970-22690989 | MS.gene08180:CDS | 45.0% | |
| AGTTGATCTGATACCTCAGG+AGG | - | chr7.3:22691571-22691590 | MS.gene08180:intron | 45.0% | |
| GGATATGAGCAAAACCGTCA+TGG | - | chr7.3:22691832-22691851 | MS.gene08180:CDS | 45.0% | |
| TGATTGACCTTGAGCCATGA+CGG | + | chr7.3:22691849-22691868 | None:intergenic | 45.0% | |
| TGCAATGAAGATGCCATTCG+TGG | - | chr7.3:22691506-22691525 | MS.gene08180:intron | 45.0% | |
| TTACTATATACCCCTCGAGC+TGG | - | chr7.3:22690711-22690730 | MS.gene08180:CDS | 45.0% | |
| ! | GATGAAGCTCAATGGTGTCA+CGG | + | chr7.3:22690966-22690985 | None:intergenic | 45.0% |
| ! | TGCACATAACCGGTCTTATG+AGG | - | chr7.3:22690903-22690922 | MS.gene08180:CDS | 45.0% |
| AGCAAAACCGTCATGGCTCA+AGG | - | chr7.3:22691839-22691858 | MS.gene08180:CDS | 50.0% | |
| GCCCTTGTGCCTTCATTAGT+AGG | - | chr7.3:22690801-22690820 | MS.gene08180:CDS | 50.0% | |
| GCCTGGAAGATGAAGCTCAA+TGG | + | chr7.3:22690974-22690993 | None:intergenic | 50.0% | |
| !! | AGTGGAGAGCGTCCATTTGA+TGG | - | chr7.3:22691437-22691456 | MS.gene08180:intron | 50.0% |
| !! | TCTTCTTCTGGTTCCTCCTG+AGG | + | chr7.3:22691587-22691606 | None:intergenic | 50.0% |
| GAGCTGGCCAAGACTTGGAA+AGG | - | chr7.3:22690727-22690746 | MS.gene08180:CDS | 55.0% | |
| CAAGTCTTGGCCAGCTCGAG+GGG | + | chr7.3:22690724-22690743 | None:intergenic | 60.0% | |
| CCAAGTCTTGGCCAGCTCGA+GGG | + | chr7.3:22690725-22690744 | None:intergenic | 60.0% | |
| TCCAAGTCTTGGCCAGCTCG+AGG | + | chr7.3:22690726-22690745 | None:intergenic | 60.0% | |
| CCCTCGAGCTGGCCAAGACT+TGG | - | chr7.3:22690722-22690741 | MS.gene08180:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 22690676 | 22691883 | 22690676 | ID=MS.gene08180 |
| chr7.3 | mRNA | 22690676 | 22691883 | 22690676 | ID=MS.gene08180.t1;Parent=MS.gene08180 |
| chr7.3 | exon | 22691811 | 22691883 | 22691811 | ID=MS.gene08180.t1.exon1;Parent=MS.gene08180.t1 |
| chr7.3 | CDS | 22691811 | 22691883 | 22691811 | ID=cds.MS.gene08180.t1;Parent=MS.gene08180.t1 |
| chr7.3 | exon | 22690676 | 22691178 | 22690676 | ID=MS.gene08180.t1.exon2;Parent=MS.gene08180.t1 |
| chr7.3 | CDS | 22690676 | 22691178 | 22690676 | ID=cds.MS.gene08180.t1;Parent=MS.gene08180.t1 |
| Gene Sequence |
| Protein sequence |
