AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene08294

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08294.t1 MTR_4g112680 96.792 530 17 0 23 552 72 601 0.0 1064
MS.gene08294.t1 MTR_4g112680 96.863 510 16 0 23 532 72 581 0.0 1024
MS.gene08294.t1 MTR_5g077610 98.540 274 4 0 279 552 251 524 0.0 558
MS.gene08294.t1 MTR_5g077610 94.578 166 9 0 23 188 73 238 4.48e-107 333
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08294.t1 AT1G72550 70.019 537 142 4 24 552 73 598 0.0 777
MS.gene08294.t1 AT1G72550 69.098 521 142 4 24 536 73 582 0.0 741

Find 142 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCCCCTACCGTGTAGTTTA+AGG 0.132100 4.2:-15478163 None:intergenic
CAGGATGTTGCCATCGCTTA+TGG 0.168492 4.2:+15474834 MS.gene08294:intron
AGTAATACCTTTATGGGTTT+TGG 0.177617 4.2:-15476204 None:intergenic
GATAGATACTGCTGAAATTT+TGG 0.197414 4.2:+15478245 MS.gene08294:CDS
TAAGTTAAGTAATACCTTTA+TGG 0.211704 4.2:-15476211 None:intergenic
AGTGGGTGATATAGCTATTT+TGG 0.214815 4.2:+15476727 MS.gene08294:CDS
GGAAAGCAAGTTGGGACTTT+TGG 0.245106 4.2:+15477352 MS.gene08294:CDS
TCAAGCAATAATCGATAATA+AGG 0.251278 4.2:+15474875 MS.gene08294:CDS
TTATGGGTTCAATGCTATTA+AGG 0.258944 4.2:+15474851 MS.gene08294:CDS
GTTTCTCTATCTTACATTAC+TGG 0.270965 4.2:+15473881 MS.gene08294:CDS
GTTTCATCTGGTGATAAATC+AGG 0.299958 4.2:+15477145 MS.gene08294:CDS
AAATCAACCTCGTAGGTAAA+AGG 0.313790 4.2:-15471531 None:intergenic
GAAGACCTATGCTTCAGTTT+TGG 0.314391 4.2:+15469430 MS.gene08294:CDS
AAGTTAAGTAATACCTTTAT+GGG 0.316491 4.2:-15476210 None:intergenic
CACTCATGATCTGGATAAAC+TGG 0.323256 4.2:+15471503 MS.gene08294:CDS
TGATTTATCCGTCTACAATA+TGG 0.331082 4.2:+15473856 MS.gene08294:CDS
CGCCATTGGCACTCATGATC+TGG 0.341241 4.2:+15471494 MS.gene08294:CDS
ATTTATAAGTAGGGATTTCC+TGG 0.342583 4.2:-15470632 None:intergenic
GGGATTACCTTTGTTTCATC+TGG 0.351003 4.2:+15477133 MS.gene08294:CDS
CATGGCAGGAAAGGAGCTTC+AGG 0.351391 4.2:+15471883 MS.gene08294:intron
TTCCTTCGAGGTGGGGCATC+CGG 0.368199 4.2:-15478516 None:intergenic
CTCTTACTATCATATATAAC+TGG 0.371043 4.2:-15472170 None:intergenic
TTGCATCCGGTTTAAGAACT+TGG 0.381322 4.2:-15474610 None:intergenic
GATGATATGCAGATAATTCA+TGG 0.381882 4.2:+15477082 MS.gene08294:intron
AAGGAGCCCGAGTTTCTTGC+TGG 0.382859 4.2:+15477304 MS.gene08294:intron
AAAATTGGTTAGAATTCAAA+GGG 0.390942 4.2:-15478540 None:intergenic
TATGACCTACTCTGCCTTGA+AGG 0.394474 4.2:+15470576 MS.gene08294:CDS
GAATGCGCACCATTGATAAT+TGG 0.394590 4.2:-15473562 None:intergenic
AGGATGTTGCCATCGCTTAT+GGG 0.398057 4.2:+15474835 MS.gene08294:intron
AACAATAGCTCTATAACTTA+AGG 0.405164 4.2:-15478193 None:intergenic
GTTTCCAATGATAACCGCTT+TGG 0.409490 4.2:-15475274 None:intergenic
TCTTCATCCATGTGATGTTA+TGG 0.411069 4.2:+15474710 MS.gene08294:CDS
TAACTTTACGCCTCTAAAAC+AGG 0.412093 4.2:+15471723 MS.gene08294:CDS
CTTTGGATTTATCATCTTTG+CGG 0.417763 4.2:-15475257 None:intergenic
TCATATATAACTGGATAAAC+AGG 0.422626 4.2:-15472161 None:intergenic
AAATCCAAAGCGGTTATCAT+TGG 0.422943 4.2:+15475270 MS.gene08294:CDS
TTTACAAGGGAAAGCAAGTT+GGG 0.428310 4.2:+15477344 MS.gene08294:CDS
GGATCACCCAAAACCCATAA+AGG 0.431211 4.2:+15476197 MS.gene08294:CDS
AGCTTCATCAAAATATTTGT+CGG 0.433457 4.2:+15470883 MS.gene08294:CDS
CCTACTCTGCCTTGAAGGAT+TGG 0.437874 4.2:+15470581 MS.gene08294:CDS
TCTACAAAGGAGCAGGGATC+AGG 0.438844 4.2:-15477696 None:intergenic
TAAATTGTCTGACATTAGTA+AGG 0.440874 4.2:+15470650 MS.gene08294:CDS
CAAGCAATAATCGATAATAA+GGG 0.444484 4.2:+15474876 MS.gene08294:CDS
TGTCAGACAATTTATAAGTA+GGG 0.445766 4.2:-15470641 None:intergenic
TCTATCTTACATTACTGGAT+TGG 0.449912 4.2:+15473886 MS.gene08294:CDS
GAAATTCTTGCATATAATTG+AGG 0.460329 4.2:+15472133 MS.gene08294:CDS
GACAGAGTAATGGAAAAGAA+TGG 0.463860 4.2:+15477112 MS.gene08294:CDS
TCTTTACCACCAATTATCAA+TGG 0.464631 4.2:+15473553 MS.gene08294:CDS
CAGTTATATATGATAGTAAG+AGG 0.467004 4.2:+15472171 MS.gene08294:CDS
CTTCGAGGTGGGGCATCCGG+TGG 0.467759 4.2:-15478513 None:intergenic
GCTTAGCATGCAATTGCATC+CGG 0.467826 4.2:-15474623 None:intergenic
TTCTTAGGTAACAGCCTTCG+CGG 0.469465 4.2:+15473760 MS.gene08294:intron
AGGTTACCAAGTTCTTAAAC+CGG 0.469844 4.2:+15474604 MS.gene08294:intron
ATGTCAGACAATTTATAAGT+AGG 0.470269 4.2:-15470642 None:intergenic
GGTTGCAATGGTCGGATTCA+CGG 0.479021 4.2:+15475182 MS.gene08294:intron
TGGTGCTAATGCGGGATTTG+AGG 0.479372 4.2:+15476802 MS.gene08294:CDS
CCCCAGGTCTTCAGACTTCG+AGG 0.481586 4.2:+15475296 MS.gene08294:CDS
GTCGAAGTGATTTCTTCTGA+TGG 0.483259 4.2:+15473815 MS.gene08294:CDS
TCAGCAGTATCTATCTGTCT+TGG 0.488220 4.2:-15478237 None:intergenic
ATATTGTAGACGGATAAATC+AGG 0.491517 4.2:-15473854 None:intergenic
GGGCTTGAGCCAATCCTTCA+AGG 0.493616 4.2:-15470590 None:intergenic
CAAAGGGGTTCCTTCGAGGT+GGG 0.495510 4.2:-15478524 None:intergenic
GTTGAGTTCTACAAAGGAGC+AGG 0.496069 4.2:-15477703 None:intergenic
CGAAGAACTCTTGTCGCCAT+TGG 0.496917 4.2:+15471480 MS.gene08294:CDS
ATTTACAAGGGAAAGCAAGT+TGG 0.498692 4.2:+15477343 MS.gene08294:CDS
ATTGGTCGGAGTATCGTTAG+AGG 0.505470 4.2:+15473904 MS.gene08294:CDS
CTTCAGGGCTGATGAGCTCA+TGG 0.507491 4.2:+15471899 MS.gene08294:CDS
CTTGACACTACTTCCTCTGC+AGG 0.508890 4.2:+15474920 MS.gene08294:CDS
AAAGGGGTTCCTTCGAGGTG+GGG 0.510499 4.2:-15478523 None:intergenic
AACCTCGAAGTCTGAAGACC+TGG 0.511782 4.2:-15475298 None:intergenic
ATGGCAGGAAAGGAGCTTCA+GGG 0.512062 4.2:+15471884 MS.gene08294:intron
ATCATCTTTGCGGTTTAGCA+TGG 0.515102 4.2:-15475247 None:intergenic
TTTCTTAGAGCAGAGTATGA+AGG 0.515713 4.2:-15475214 None:intergenic
TCAGAAGAAATCACTTCGAC+AGG 0.520858 4.2:-15473812 None:intergenic
GGCGTAAAGTTAATACTAGA+AGG 0.521759 4.2:-15471712 None:intergenic
AATTCAAAGGGGTTCCTTCG+AGG 0.522353 4.2:-15478528 None:intergenic
CTATACTGTGGTGCTAATGC+GGG 0.524427 4.2:+15476794 MS.gene08294:CDS
TGAAGAACATCACTTCTAGT+AGG 0.530614 4.2:-15474693 None:intergenic
ACAGAGTAATGGAAAAGAAT+GGG 0.533786 4.2:+15477113 MS.gene08294:CDS
GTTGTATGTGCTGTATTAAG+AGG 0.534713 4.2:+15470810 MS.gene08294:CDS
CATAATATATTCAGGCTGTA+CGG 0.537286 4.2:+15476130 MS.gene08294:intron
AAATTTCAGGTTGCAATGGT+CGG 0.537370 4.2:+15475174 MS.gene08294:intron
AGTATAGTGCAGCAAGTTGG+CGG 0.538109 4.2:-15476778 None:intergenic
TTCGAGGTGGGGCATCCGGT+GGG 0.539071 4.2:-15478512 None:intergenic
GTTCTTTGTCTCCAGAGTGA+TGG 0.544499 4.2:-15473587 None:intergenic
AAGCCTTAAACTACACGGTA+GGG 0.545734 4.2:+15478160 MS.gene08294:CDS
TGGCTTGGTGGACAGAGTAA+TGG 0.547034 4.2:+15477102 MS.gene08294:CDS
CCTGGAACCCACAGAAAACT+CGG 0.547117 4.2:-15470614 None:intergenic
TTGTCTACCAGCAAGAAACT+CGG 0.549695 4.2:-15477311 None:intergenic
TCAAAGGATTCTTGTTACCA+AGG 0.552956 4.2:+15478480 MS.gene08294:intron
TTCAATGTTGAGTTCTACAA+AGG 0.554393 4.2:-15477709 None:intergenic
GAGCGATCTTATCAGAAAAG+AGG 0.555737 4.2:+15474947 MS.gene08294:CDS
CACCGGATGCCCCACCTCGA+AGG 0.556777 4.2:+15478514 MS.gene08294:CDS
CAATTCCAAAACTGAAGCAT+AGG 0.560611 4.2:-15469435 None:intergenic
AATACAGCACATACAACATA+CGG 0.563509 4.2:-15470804 None:intergenic
ACCTCGAAGTCTGAAGACCT+GGG 0.564197 4.2:-15475297 None:intergenic
GCAGATAATTCATGGCTTGG+TGG 0.572739 4.2:+15477090 MS.gene08294:intron
TCAAAGGGGTTCCTTCGAGG+TGG 0.572743 4.2:-15478525 None:intergenic
TATGCAGATAATTCATGGCT+TGG 0.573431 4.2:+15477087 MS.gene08294:intron
CGGTTATCATTGGAAACCCC+AGG 0.575178 4.2:+15475280 MS.gene08294:CDS
ACAAGCTAGAATTATTTACA+AGG 0.578446 4.2:+15477330 MS.gene08294:CDS
TGCGCATTCAGCCATCACTC+TGG 0.580971 4.2:+15473576 MS.gene08294:CDS
ATCCAGATCATGAGTGCCAA+TGG 0.581047 4.2:-15471496 None:intergenic
CACAGTATAGTGCAGCAAGT+TGG 0.583950 4.2:-15476781 None:intergenic
CCGGTGGGAAGAAAATTCCT+TGG 0.585396 4.2:-15478497 None:intergenic
GAAGCCTTAAACTACACGGT+AGG 0.585580 4.2:+15478159 MS.gene08294:CDS
TCTTACATTACTGGATTGGT+CGG 0.588320 4.2:+15473890 MS.gene08294:CDS
AGTATCGTTAGAGGCAGAAG+AGG 0.589218 4.2:+15473913 MS.gene08294:CDS
ACTATACTGTGGTGCTAATG+CGG 0.590221 4.2:+15476793 MS.gene08294:CDS
CAAGCTAGAATTATTTACAA+GGG 0.593489 4.2:+15477331 MS.gene08294:CDS
GTACGGACTAGTCTTATGCC+TGG 0.599777 4.2:+15476147 MS.gene08294:CDS
GAAAACTGTTGCTCACAACA+AGG 0.602961 4.2:+15476176 MS.gene08294:CDS
AAGCAATAATCGATAATAAG+GGG 0.604733 4.2:+15474877 MS.gene08294:CDS
AGCCTTAAACTACACGGTAG+GGG 0.609239 4.2:+15478161 MS.gene08294:CDS
TCATCCATGTGATGTTATGG+AGG 0.610955 4.2:+15474713 MS.gene08294:CDS
TGTCTACCAGCAAGAAACTC+GGG 0.611259 4.2:-15477310 None:intergenic
CCAATCCTTCAAGGCAGAGT+AGG 0.611521 4.2:-15470581 None:intergenic
AGAAACTTCCATATTGTAGA+CGG 0.611613 4.2:-15473864 None:intergenic
TATTGTAGACGGATAAATCA+GGG 0.617329 4.2:-15473853 None:intergenic
TTGAAAGAAATCAACCTCGT+AGG 0.620527 4.2:-15471538 None:intergenic
AATCAGGATATTATATTGAG+CGG 0.621082 4.2:+15477161 MS.gene08294:CDS
AGCATTGAACCCATAAGCGA+TGG 0.622930 4.2:-15474844 None:intergenic
CTGCAGAGGAAGTAGTGTCA+AGG 0.624219 4.2:-15474919 None:intergenic
TCATGATCTGGATAAACTGG+AGG 0.627307 4.2:+15471506 MS.gene08294:CDS
TTTATCACCAGATGAAACAA+AGG 0.630360 4.2:-15477140 None:intergenic
AGAACATCACTTCTAGTAGG+AGG 0.638419 4.2:-15474690 None:intergenic
CACTGCTACTGATCTGACAA+AGG 0.639733 4.2:+15473624 MS.gene08294:CDS
TAAAATACATATAAAGCCAG+AGG 0.642113 4.2:+15470683 MS.gene08294:CDS
TTGAGTTCTACAAAGGAGCA+GGG 0.644199 4.2:-15477702 None:intergenic
GAACCCACAGAAAACTCGGA+GGG 0.649688 4.2:-15470610 None:intergenic
CAACTTGCTGCACTATACTG+TGG 0.650393 4.2:+15476782 MS.gene08294:CDS
TGCGCACCATTGATAATTGG+TGG 0.652799 4.2:-15473559 None:intergenic
AGATCGCTCAGCTCCTGCAG+AGG 0.653077 4.2:-15474933 None:intergenic
AGTACCTCCATAACATCACA+TGG 0.655899 4.2:-15474717 None:intergenic
CATGATCTGGATAAACTGGA+GGG 0.664709 4.2:+15471507 MS.gene08294:CDS
GTAAAGTTAATACTAGAAGG+TGG 0.672394 4.2:-15471709 None:intergenic
ATCAGAAGCCTTAAACTACA+CGG 0.672657 4.2:+15478155 MS.gene08294:CDS
CCTCGAAGTCTGAAGACCTG+GGG 0.674003 4.2:-15475296 None:intergenic
TGCAATGGTCGGATTCACGG+AGG 0.701182 4.2:+15475185 MS.gene08294:CDS
CAAAGATGATAAATCCAAAG+CGG 0.701337 4.2:+15475260 MS.gene08294:CDS
GGAACCCACAGAAAACTCGG+AGG 0.726992 4.2:-15470611 None:intergenic
AAATTGGTTAGAATTCAAAG+GGG 0.754601 4.2:-15478539 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr4.2 gene 15469291 15478554 15469291 ID=MS.gene08294
chr4.2 mRNA 15469291 15478554 15469291 ID=MS.gene08294.t1;Parent=MS.gene08294
chr4.2 exon 15469291 15469299 15469291 ID=MS.gene08294.t1.exon1;Parent=MS.gene08294.t1
chr4.2 CDS 15469291 15469299 15469291 ID=cds.MS.gene08294.t1;Parent=MS.gene08294.t1
chr4.2 exon 15469416 15469471 15469416 ID=MS.gene08294.t1.exon2;Parent=MS.gene08294.t1
chr4.2 CDS 15469416 15469471 15469416 ID=cds.MS.gene08294.t1;Parent=MS.gene08294.t1
chr4.2 exon 15470575 15470704 15470575 ID=MS.gene08294.t1.exon3;Parent=MS.gene08294.t1
chr4.2 CDS 15470575 15470704 15470575 ID=cds.MS.gene08294.t1;Parent=MS.gene08294.t1
chr4.2 exon 15470789 15470904 15470789 ID=MS.gene08294.t1.exon4;Parent=MS.gene08294.t1
chr4.2 CDS 15470789 15470904 15470789 ID=cds.MS.gene08294.t1;Parent=MS.gene08294.t1
chr4.2 exon 15471479 15471545 15471479 ID=MS.gene08294.t1.exon5;Parent=MS.gene08294.t1
chr4.2 CDS 15471479 15471545 15471479 ID=cds.MS.gene08294.t1;Parent=MS.gene08294.t1
chr4.2 exon 15471703 15471744 15471703 ID=MS.gene08294.t1.exon6;Parent=MS.gene08294.t1
chr4.2 CDS 15471703 15471744 15471703 ID=cds.MS.gene08294.t1;Parent=MS.gene08294.t1
chr4.2 exon 15471891 15471932 15471891 ID=MS.gene08294.t1.exon7;Parent=MS.gene08294.t1
chr4.2 CDS 15471891 15471932 15471891 ID=cds.MS.gene08294.t1;Parent=MS.gene08294.t1
chr4.2 exon 15472116 15472192 15472116 ID=MS.gene08294.t1.exon8;Parent=MS.gene08294.t1
chr4.2 CDS 15472116 15472192 15472116 ID=cds.MS.gene08294.t1;Parent=MS.gene08294.t1
chr4.2 exon 15473543 15473669 15473543 ID=MS.gene08294.t1.exon9;Parent=MS.gene08294.t1
chr4.2 CDS 15473543 15473669 15473543 ID=cds.MS.gene08294.t1;Parent=MS.gene08294.t1
chr4.2 exon 15473767 15473934 15473767 ID=MS.gene08294.t1.exon10;Parent=MS.gene08294.t1
chr4.2 CDS 15473767 15473934 15473767 ID=cds.MS.gene08294.t1;Parent=MS.gene08294.t1
chr4.2 exon 15474606 15474734 15474606 ID=MS.gene08294.t1.exon11;Parent=MS.gene08294.t1
chr4.2 CDS 15474606 15474734 15474606 ID=cds.MS.gene08294.t1;Parent=MS.gene08294.t1
chr4.2 exon 15474837 15474968 15474837 ID=MS.gene08294.t1.exon12;Parent=MS.gene08294.t1
chr4.2 CDS 15474837 15474968 15474837 ID=cds.MS.gene08294.t1;Parent=MS.gene08294.t1
chr4.2 exon 15475183 15475317 15475183 ID=MS.gene08294.t1.exon13;Parent=MS.gene08294.t1
chr4.2 CDS 15475183 15475317 15475183 ID=cds.MS.gene08294.t1;Parent=MS.gene08294.t1
chr4.2 exon 15476144 15476218 15476144 ID=MS.gene08294.t1.exon14;Parent=MS.gene08294.t1
chr4.2 CDS 15476144 15476218 15476144 ID=cds.MS.gene08294.t1;Parent=MS.gene08294.t1
chr4.2 exon 15476719 15476823 15476719 ID=MS.gene08294.t1.exon15;Parent=MS.gene08294.t1
chr4.2 CDS 15476719 15476823 15476719 ID=cds.MS.gene08294.t1;Parent=MS.gene08294.t1
chr4.2 exon 15477094 15477189 15477094 ID=MS.gene08294.t1.exon16;Parent=MS.gene08294.t1
chr4.2 CDS 15477094 15477189 15477094 ID=cds.MS.gene08294.t1;Parent=MS.gene08294.t1
chr4.2 exon 15477307 15477390 15477307 ID=MS.gene08294.t1.exon17;Parent=MS.gene08294.t1
chr4.2 CDS 15477307 15477390 15477307 ID=cds.MS.gene08294.t1;Parent=MS.gene08294.t1
chr4.2 exon 15477675 15477739 15477675 ID=MS.gene08294.t1.exon18;Parent=MS.gene08294.t1
chr4.2 CDS 15477675 15477739 15477675 ID=cds.MS.gene08294.t1;Parent=MS.gene08294.t1
chr4.2 exon 15478152 15478275 15478152 ID=MS.gene08294.t1.exon19;Parent=MS.gene08294.t1
chr4.2 CDS 15478152 15478275 15478152 ID=cds.MS.gene08294.t1;Parent=MS.gene08294.t1
chr4.2 exon 15478486 15478554 15478486 ID=MS.gene08294.t1.exon20;Parent=MS.gene08294.t1
chr4.2 CDS 15478486 15478554 15478486 ID=cds.MS.gene08294.t1;Parent=MS.gene08294.t1
Gene Sequence

>MS.gene08294

ATGCCTACCGTGAGTTTAGGGCGGGATCGACTGTTTGCAGCTCTAGGAAAAACGTACAGTAAGTCACCTTCCATTTCTCCTTTCAATCAATCTTCAATTATTTTCATGAATTTCCATTTCTCCAGCACAAGATGAATTTGAAGACCTATGCTTCAGTTTTGGAATTGAGCTTGACGATGTTGTAAGTTATTTTCCCTTTTTATTTCCAACTCCAACTAATTATCATCATAATAATCTCATTAATTAATTAATTAATACACTGTCGTTGTATATTAGACTACAGAAAAAGCAATTGTAAGAAAAGAGAAGCATTTAGAACAAGAAGAAGCTGATGAGGATGAAGAAGTTATCTATAAAATTGAAATTCCTGCTAATAGGTACCCACCCCTATCATTATAGTCCCTGCAATTATTGCGTTTTTATCGTTTTAGCCTTTTTTTTTAAGTGTTTTTGTTTTGGTTCCGTCCCTGCAAATTCTAAAACAATTGTTTTTAGCCCCTGAAGTCATAGGTTGCACAATATAATCACGCCACGTGGACATTAGGGACCAAAACCAACAATTTTTAAATTTGCAGGGATGAAACCCAAACAAAAAAACTTGTGCGGACTAAAACCAAATCCTTACAATTGCAGGGACTAAAATCATATTTAAACCTATATTTTATGTTTATGTTGCAGATGTGAATCAACAAAATTTGTTAATGACATGATTGAGTAAATGTGGTTACTCCAATCTAAATTTTTTATGTGTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTTTATTATCCTCTTATTTATTTTTCTTTGATTTGGAATTCATAATCTATGTTGCATTTGAAATAAGCTTTTGAGTACATTAGCGGAGATTGACAATATTGATACTTAGTTAAACTGGAATTAATGGAATACTGGTTGCTTCTCTTTTTTTTTTTTTTTATCAAGTAGTTTAGTGGCTAAAAATTCACCCCTTAAATGAATAAGTGGGGAACCCCGGCCCCTACATTCTGTAGTATCCCTGCCAACTGAGCTATGCTCACGGGAACATAGAGCTTGCTTTCTATTGTTGGAAGGAATACTTATTATCAATGATGTGGCACATCATTTTCTCATAGCAATTGCAAAGTCACTTAATTATGTAACCATTTGAAGCACCTCATCACACATTGTCATTGCTTTGAAATCATATGTTGAAAACGTGTTAGTTTTCTGTAAAGCAAAATGTCATGCTGAATTGCTGTTTACTTTTTGCTCAGATATGACCTACTCTGCCTTGAAGGATTGGCTCAAGCCCTCCGAGTTTTCTGTGGGTTCCAGGAAATCCCTACTTATAAATTGTCTGACATTAGTAAGGATGCAATGCATAAAATACATATAAAGCCAGAGGTAGATATGCTGTACATATACACAATTTTTTAGTACAAGTCTTTCCTAAGTGGAAAATGAATTGTTTCTTTTGTCTCATTGCAGACATCTCTAATTCGTCCGTATGTTGTATGTGCTGTATTAAGAGGTTTAACTTTTGATAAAGCGAGATACAACAGCTTTATTGATCTCCAAGACAAGCTTCATCAAAATATTTGTCGGTAATGTGATTTGAATCGCCACTTTATTTGTTTCATGCTGCTCATTACCTGTTTCTCAAATCAGTGATGTACTTGCTCCTATCTGTTTACTTAAAAAATTTAGAGCCATATTTAGTCAAATATGCTTCTGTAATAGACTTCTAGTGAATGATAATTTTGTGTTACAACAGTTGTAATTTTCTGTGGATTGAATAGCTTGCAAAGCAAGCCGTTGTAGCTGTATAAATTAGCTTAGCAGAATTCATACGTTTGGGTAATTTTCTGTAAAACAAATCCATCGCCTAAATTTTGTCTCTACCTTTCTCTCTGTCTCATTCTCTCTAGATTCTTCAAACCAAAATAGCCTTGTGAGTTTGAATGCTGTCATGTGTGCCTTTATTTTCTCCTTATCGAGTTTTTGATATCATACATAATTTTTTAGTGCATAAAAGGTTATTTCCGTACTCTGAGCTGAGTTTTGTTTATATTGTATATATGTATATCATCTCATTAGTTTATTTTTATGTTTTTGTTATCTCATATCATTGCACTTTAAGAATGATTGTTCAATTAATTTGTTTTCATTCTGTTTCAGGCGAAGAACTCTTGTCGCCATTGGCACTCATGATCTGGATAAACTGGAGGGTCCTTTTACCTACGAGGTTGATTTCTTTCAAACTCAGGAGCCCCGTATTTGAATTCTTTTAAGGCAATTTAACAAAGAGAGATCTTAATTGCCATTTTCTTACTAATTTGATTATATCTTGTTCTCTAGGTCATCAACATAATGTTTTCTCTCATGTTTTTTTCTTTTGCCAGGCTTTACCACCTTCTAGTATTAACTTTACGCCTCTAAAACAGGTAACTTTATTTGGGGAATTCATTTTCTATTAAAGCATATGTTTGAAATCACGTGAGAAAAAAAATGTTTTTGGTCTATGAATTTATTAATCAAGCTGTAAATCAAACATTTAATGATTTTTCTTCCTCTGTTCTTGGCATGGCAGGAAAGGAGCTTCAGGGCTGATGAGCTCATGGAGTTTTACAAAGTGAGTTTTTTGAACATTGTTTCCTTGCTTTATATATATAGTATAAGTTGATATGAACCTAGCACGAGTAAGCTTATGAGTCATTCTAGTCCACCCAACAAGGTGTTCATTTATTCTGTTTTTCTGTTTGGACATACTTCCTTTGAGATGCGTCTGAATACTTTTTTGATTTAATTTTGACAGTCAGATTTGAAACTGAAGAAATTCTTGCATATAATTGAGGACTCTCCTGTTTATCCAGTTATATATGATAGTAAGAGGTATTATATTTTCATTGTATCCCCATTTTTAAATTTGCAAAACTTTGAAATCTTTTAGCTCTCATTCTGGTAATACTAATTTACCACAAAGCTTTGTTTGTATTTAGGCATATTATTATTTGTACTATTATTGTTGCGTACGGATGGGAATGCATGGCTGCATGCTAATTTACATTACACTAAACTAATAATAAAATTGTGAATGGATGTTACAAAAAGACATTGGTGTGTAAATGCTTGTTATGATTCTAATTTAGACTATGTTAAATAATGTTCAGGATGTATGAACAAAGTATAATTATAAAATTTATGGCATGGTGATTAAGAATTTATTTTCAAACACGTTCTATGTTTTTAATTTCTAAACAAGAAAAAATCATGCTAGTTATTCTAAAATTCTATATACTGAACTTCTAAAGACTTCTCAGAACATGTTAACTTTTAAGTGAAGTATTAAAGTTTTTAATAATATCAGAGCATCCTATTGCCATTGGTTGCATGTTATTTTTTTAGTTTTGGTATTGCTTGGCATTAGAAGATAGCGGCATTGGTGGTGACTCAAATCTGTCTATTATCTAACATGGCCTAGGGTACCTTCTTTTCTTTGGGAGGAACCATGTCTTTGTGCATAGTTTTGTTCTACTTCTTATTGATGTGTACGGTAGGGGGGTTGATTTCACGCCTTGAGATTTTTCACTGAAAATTCAGAGGTGATTGAGTTACTTCTTCCATGAAAGCAGAGAATTTGATGTTTCTATACAAGAAATATGACTTTTTGGATTTACCTTTAATATGAAGCGATGTTTGCACAGTCAGTGCCTGAGAATTGGGCCAATTTGTTTCGTTTGTTTTAGTTGGTGGGGATCCTCGTCTTCCACTAGTTATATTATTTCTCCTCTCTCTCTCTCTAATAAAATGTCACTTTCACAAGACACCCTACCTGTTGAAATTCTAATTTATTTTAAATATTTCAGATTTAAAGCATATAATATACTCATTGTATGTTTTGCAAAGATGTAATAAATTTCTACATTGAAAAAGTTAAGTAGGTTTGTACATTTGAATTTTTACTCATGACACACTGACACGTGAATTGTAGTCATTTAATTTACAGATGCATACACTGAAAAGACTTGTCAAATATTCTTCAATGAGATGCTTTTCCACTGAATTTTGGTCTGGTTAATTTGTAGAAATGCTTTTAATTATGGCCCCTTCTCTATTTTCTGTTAATTGCACTTTTTTTGTAAATGAAAAATTGTGACATGTGGCCTTTAATATTCAGTAATGCTGTTGCTTTGTATATGCTGTTACGAATTCAGTTATTGATATGCTATGAATTTTTGACAGAACTGTTTTATCTTTACCACCAATTATCAATGGTGCGCATTCAGCCATCACTCTGGAGACAAAGAACATATTTATTGAATGCACTGCTACTGATCTGACAAAGGCAAAAATTGTTCTAAATACAATGGTATGGTAGAGCAGTGAATTTTGCTGACCTTTTTTGTTTACCTTTTCTTGGTGTTACAATATTTCGGATTACGACAGACTATTCTCTGATTTCTTAGGTAACAGCCTTCGCGGAATATTGTGAAAATAAGTTTGTCATTGAACCTGTCGAAGTGATTTCTTCTGATGGAAAATCTCACATATACCCTGATTTATCCGTCTACAATATGGAAGTTTCTCTATCTTACATTACTGGATTGGTCGGAGTATCGTTAGAGGCAGAAGAGGTAATTATTTCCTATAAATTTATTGAGAAGTACGTTCTTTCATATTGACTGCTTGTAAGCTTTCTATCATTAATTAGGCTTCAGGCATACATTGATCTTTTCCTTTATAAGTTATATTTACAATTGGTGATTATACTTACTATACATGTTCAGTATTTGTTGGATCATCTTTGTTATCGTTATTAGTGAGTTATGACTCTAGTCTAGATCATTAACTTTTTTGGGGTTTTGTTCACAGTAGTGTGTGTGTCGCTTTTCTTTATACAATTAATCTTTCTGCATTTCATATTTGACAGTTATTTTATTTTATTTTTAAATAAAAGAGTTTTCTCCTGCTGTTCTTTCCAGATTTTAATTTACAGCATGAAATATTTTTACCTGCTAATTTATTTCTCGGACTAAAGGTTTGCATGTGTATAAACAATCAAGAAAAAAATATTTTTAAGATCTGTACATAATACAGGGGGACTACGTATTCCTTTGTTTGGTCTTTTTACTTCTATGGTTGAGTTCTTCAGATGTTCGTGTCATTATTACCTTAAGTTCTATTTTGCTAGGCTTTTTATGTGCAGTGTATTGTCTTTAATGCAAGATTCAATGATTATGTTTTCTGATTGAAACTGAAATTAATATTGTAATTCTTGTGCCATAATTTTTCCCAATTTTTTCAGGTTACCAAGTTCTTAAACCGGATGCAATTGCATGCTAAGCAATCTACATCTGACAATAAACAGTGCAAATTTATTATATCTGTACCTCCTACTAGAAGTGATGTTCTTCATCCATGTGATGTTATGGAGGTACTGGCAGTGATACACTTAATCATTCACAGATGCATATTGGATCTATTACATATATGTTTTACAGACAAGGCACCCCTCTTTTGGTTCTTTTATCTGCAGGATGTTGCCATCGCTTATGGGTTCAATGCTATTAAGGATCAAGCAATAATCGATAATAAGGGGTCTAAGAGATTAGCTGCTTCCTTGACACTACTTCCTCTGCAGGAGCTGAGCGATCTTATCAGAAAAGAGGTATAGAACAAAAATATAGATCTTGTATCTCCTATAATTGAAGCATGTTAAAGTTTCATGCTTTTGATTTTCTTTAAAATATGCCAATCAGTTTATGGTGTTTGTTAAAACTAATGCAACAGAATAAATTACTACCTGAAATATTCTTGAATGATATCGTTAATTATTTGAAGTGCCTAGTTACTTGAATCCTTTTATTGCCAAAAAATTTCAGGTTGCAATGGTCGGATTCACGGAGGTTTTGACCTTCATACTCTGCTCTAAGAAAGAAAACTTTTCCATGCTAAACCGCAAAGATGATAAATCCAAAGCGGTTATCATTGGAAACCCCAGGTCTTCAGACTTCGAGGTTCATACACTTCAACTCTTTTAATTGAAATAACTACAGAATCTCTTAAAATGGTTATTTTCTGCAGTGATCTTTTTGCATTATTCTGAGGTTGTTATTCCAGTTGCTGGATAAACTACTAGTATTCACAGTTTCTTTACATAGCTCTTTCAATAGCTTTTATTTTGTTAATATTTCTTTTTCTTTAATGTTTGATTTGGTGGAGTCACTCGGTTTTCATGATCTGTTGTATACAGTATACACATGTATAAAAATGTTTTCCCCTAGAAACACTGTTCATTCTGCTTTTCATCCAAAAAATAGAAACAAAAATAATTGTCATGGAATACATCGCAAGACAGCTGTGCTGCTTCATCCCGGCTACCATCTACCAATCAGTTTGTCAAATCTGATGGATTTTTTTTCCTCTCATTTTGACATGCTTTTCCTTTATATTTGTACTGCTGCATGGGTTATACTCCCACTTTCCTGTTTTTAATTAATATCTGAAGTTCACATTCTCAACTGGATCAAACACTTGTATTTTATCTGCAGTCTGCTCATTTGTGTTGACCTGGCATTTTGGTGGGCCCTCTATGTTGATGGTGTTTATTGCTTTAACCATCCGTTCTGAGCACTTAAGATGCAGTCTATTCTATATGTTGATAATCTAATCTGTATTTGTTTTTAGTCTTATATTTATTTTATTTTTTAAAGTTGAGCGTATAACTGGATAATTGTATTTATGAGTGATGTAGTGGTTTTTCTCCATGTTCAAATTTATATTTGATCTTTAGTTTAAGTTTTTGCTTTAATTTAATTTTATGTTTTTACATAATATATTCAGGCTGTACGGACTAGTCTTATGCCTGGTATTTTGAAAACTGTTGCTCACAACAAGGATCACCCAAAACCCATAAAGGTATTACTTAACTTATTCCGTTTGGTCATAGATCTTGTTATCATGATCCTATGCAGTAAGTATATTCTAGGAAAGTGTGATACTCCCATGAAAATTCAGTTCCATTGATACAAAGAAAAGTGTTTATCGTCTTTATTTTTTGCTTTTAGTTTACGTACATATTAATTATTGGTCAGTGCCAACTTCTTCCTTTTAAAAAAGATTGTGATATTATTAGTATCTTACGCTTCAAGTCTATTGTCAAATGTATAATCAGTTGATTTTGATGTATCTTTAAATTTCCTTTATTTCTGAAATCGACAACTAAGGCTAGTCTGCTAGTGCTATCTTCTTCCAATGGCTTGATAATATGTTAGCATGTGCAAATTTGAAAGATTTAAACTTCAGTTAGACAAAATTGATCAACTCTACCTCTGCATCAGATAATTGAATTTATCATTTTTCTTAACTCTACTATTTTGTAAGCTTATATGAATATGTTTTGAAAAATTTTGGTTCAGATTTTTGAAGTGGGTGATATAGCTATTTTGGATGACAATGATGTCGGTGCAAAAAATCTCCGCCAACTTGCTGCACTATACTGTGGTGCTAATGCGGGATTTGAGGTATATTTTGTAATGACTTCATGGCAGTTTATTCTGTTTTTCGCTCGTTGAATACATCACTAGCTCCACTTGATTGATTATAGATAAGAATGAGTGAACCTAGATTTTTCTTATTCAAAACAGCTTAGAATTTAATTTTCACTTACTATAATTTAACAAAGTGTCTTTAAAATAAATATTGCTACTTCTCACAGTTAGAATACATTCTACCTAGATGAGAGAATAATAAATTGAATCATTGTACTTAAATTTATCAATGATGATATGCAGATAATTCATGGCTTGGTGGACAGAGTAATGGAAAAGAATGGGATTACCTTTGTTTCATCTGGTGATAAATCAGGATATTATATTGAGCGGTCAGATGTAAGTGTATATTAATTACTACATAGGATCCCTCGGTTTTTTATTTTGTCCTTTCCTTTGCTAGGAACACTTATTCTGCTTCTGACTTCTAAATTATGAACTCGACTCTTACTGAAGGAGCCCGAGTTTCTTGCTGGTAGACAAGCTAGAATTATTTACAAGGGAAAGCAAGTTGGGACTTTTGGCATTGTTCATCCAGAGGTATTCGTGATCACTGAAATTGTGTCATCTTGATTTAATATGGCCAATTGTTTGGTACATAATTTTGCATTCCTGTTTTTTTTTAATATATTCTGGTAACTGTATATTTTCTTGAATCAAGTCATATGCAATGGAATTTTTATGAGTTAAAATGATAACCAAAGGGACATTTGCTTAAGTATTCAATTTTCCCGTTGAGTAGATTGTATCTCTGGGAACAAATGCCTCTGTATATGTAGTTGGAGTCATATACAAATTATTCTGCTTGTTGTGTATTCTTTCAGGTATTAAACAACTTCGACATTCCTGATCCCTGCTCCTTTGTAGAACTCAACATTGAAAGTTTCTTGTAAGGATTATTTGAAACAAATGTAAGTGTCTCCATCCTAATCTATTTCCTATGGTCAGCCTTTGATGATGCAGCTTTGTACTTTTCCACGTTTTTTGCTCGTCTGTATCTTTCACCCCTCTTCCCCGACTACTGCATCATCTCCTCCGTCAAAATTTTGTTGGACTGATGTTAACTTTGTTTATAGGTTGCCATTGCCCCCTAGATGTTTTGAGTTTATCATTAATTCAATCTGGGAATCAAGATGCCCAGCTAGTTGAGTGACTATTCAATTGCCTTACCCGGTTCTTGGGATTTAGAAATACATTCCAGCAGCAGAATGTAACTTATCTGCCCTAGATCAAGGTTGTTTTGTTGATTATACAATTCTACCATTTTCTTTGTTTGAAGCTATTCAATTTCTTGCGCTCAGAACATCAGAAGCCTTAAACTACACGGTAGGGGATAATTCTACCTTAAGTTATAGAGCTATTGTTGAAGACAATTTGTCCTTAAAACCAAGACAGATAGATACTGCTGAAATTTTGGCGAATACAGTAAGAGTTGTCACATAGACATTTCTAACACTTATGTCTGCTTCAGTTTATAGTATTTGTTTAAGATTGTAGGATGTTTATGTTTGTTCTATTTGTGTTGACGCCATAAAGTTTGAGCTTGATGTTTGTTAGTGATCTTACTATATGAAAAACGGGTTGGTACCTCAACATTTTTTGTACTCCTCTTAACTCTCATTGTGTTTATCAAAGGATTCTTGTTACCAAGGAATTTTCTTCCCACCGGATGCCCCACCTCGAAGGAACCCCTTTGAATTCTAA
Protein sequence

>MS.gene08294.t1

MPTHKMNLKTYASVLELSLTMLYDLLCLEGLAQALRVFCGFQEIPTYKLSDISKDAMHKIHIKPETSLIRPYVVCAVLRGLTFDKARYNSFIDLQDKLHQNICRRRTLVAIGTHDLDKLEGPFTYEALPPSSINFTPLKQERSFRADELMEFYKSDLKLKKFLHIIEDSPVYPVIYDSKRTVLSLPPIINGAHSAITLETKNIFIECTATDLTKAKIVLNTMVTAFAEYCENKFVIEPVEVISSDGKSHIYPDLSVYNMEVSLSYITGLVGVSLEAEEVTKFLNRMQLHAKQSTSDNKQCKFIISVPPTRSDVLHPCDVMEDVAIAYGFNAIKDQAIIDNKGSKRLAASLTLLPLQELSDLIRKEVAMVGFTEVLTFILCSKKENFSMLNRKDDKSKAVIIGNPRSSDFEAVRTSLMPGILKTVAHNKDHPKPIKIFEVGDIAILDDNDVGAKNLRQLAALYCGANAGFEIIHGLVDRVMEKNGITFVSSGDKSGYYIERSDEPEFLAGRQARIIYKGKQVGTFGIVHPEVLNNFDIPDPCSFVELNIESFLTSEALNYTVGDNSTLSYRAIVEDNLSLKPRQIDTAEILANTDSCYQGIFFPPDAPPRRNPFEF