Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050117.t1 | RHN42411.1 | 98.1 | 582 | 11 | 0 | 1 | 582 | 21 | 602 | 0.00E+00 | 1139.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050117.t1 | Q9SGE9 | 71.0 | 590 | 151 | 5 | 1 | 582 | 21 | 598 | 4.3e-242 | 838.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050117.t1 | A0A396GQF4 | 98.1 | 582 | 11 | 0 | 1 | 582 | 21 | 602 | 0.0e+00 | 1139.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050114 | MS.gene050117 | 0.904987 | 6.91E-80 | -1.69E-46 |
| MS.gene050116 | MS.gene050117 | 0.866033 | 3.83E-65 | -1.69E-46 |
| MS.gene050117 | MS.gene08294 | 0.937665 | 2.39E-98 | -1.69E-46 |
| MS.gene050117 | MS.gene34907 | 0.914184 | 2.58E-84 | -1.69E-46 |
| MS.gene050117 | MS.gene47551 | 0.802734 | 4.87E-49 | -1.69E-46 |
| MS.gene050117 | MS.gene70530 | 0.81556 | 8.68E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 126 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGGATGTTGCCATCGCTTA+TGG | 0.168492 | 4.4:-17174823 | MS.gene050117:CDS |
| AGTAATACCTTTATGGGTTT+TGG | 0.177617 | 4.4:+17173453 | None:intergenic |
| TAAGTTAAGTAATACCTTTA+TGG | 0.211704 | 4.4:+17173446 | None:intergenic |
| AGTGGGTGATATAGCTATTT+TGG | 0.214815 | 4.4:-17172930 | MS.gene050117:CDS |
| GGAAAGCAAGTTGGGACTTT+TGG | 0.245106 | 4.4:-17172305 | MS.gene050117:CDS |
| TCAAGCAATAATCGATAATA+AGG | 0.251278 | 4.4:-17174782 | MS.gene050117:CDS |
| TTATGGGTTCAATGCTATTA+AGG | 0.258944 | 4.4:-17174806 | MS.gene050117:CDS |
| GTTTCTCTATCTTACATTAC+TGG | 0.270965 | 4.4:-17175776 | MS.gene050117:CDS |
| GTTTCATCTGGTGATAAATC+AGG | 0.299958 | 4.4:-17172512 | MS.gene050117:CDS |
| AAATCAACCTCGTAGGTAAA+AGG | 0.313790 | 4.4:+17178127 | None:intergenic |
| GAAGACCTATGCTTCAGTTT+TGG | 0.314391 | 4.4:-17180180 | MS.gene050117:CDS |
| AAGTTAAGTAATACCTTTAT+GGG | 0.316491 | 4.4:+17173447 | None:intergenic |
| CACTCATGATCTGGATAAAC+TGG | 0.323256 | 4.4:-17178155 | MS.gene050117:CDS |
| TGATTTATCCGTCTACAATA+TGG | 0.331082 | 4.4:-17175801 | MS.gene050117:CDS |
| CGCCATTGGCACTCATGATC+TGG | 0.341241 | 4.4:-17178164 | MS.gene050117:CDS |
| ATTTATAAGTAGGGATTTCC+TGG | 0.342583 | 4.4:+17179026 | None:intergenic |
| GGGATTACCTTTGTTTCATC+TGG | 0.351003 | 4.4:-17172524 | MS.gene050117:CDS |
| CATGGCAGGAAAGGAGCTTC+AGG | 0.351391 | 4.4:-17177774 | MS.gene050117:intron |
| CTCTTACTATCATATATAAC+TGG | 0.371043 | 4.4:+17177487 | None:intergenic |
| TTGCATCCGGTTTAAGAACT+TGG | 0.381322 | 4.4:+17175047 | None:intergenic |
| GATGATATGCAGATAATTCA+TGG | 0.381882 | 4.4:-17172575 | MS.gene050117:intron |
| AAGGAGCCCGAGTTTCTTGC+TGG | 0.382859 | 4.4:-17172353 | MS.gene050117:CDS |
| AAATTGAAATTCCTGCTAAT+AGG | 0.389907 | 4.4:-17179968 | MS.gene050117:intron |
| AACATTGAAAGTTTCTTGTA+AGG | 0.390477 | 4.4:-17171934 | None:intergenic |
| TATGACCTACTCTGCCTTGA+AGG | 0.394474 | 4.4:-17179082 | MS.gene050117:CDS |
| GAATGCGCACCATTGATAAT+TGG | 0.394590 | 4.4:+17176095 | None:intergenic |
| AGGATGTTGCCATCGCTTAT+GGG | 0.398057 | 4.4:-17174822 | MS.gene050117:CDS |
| GTTTCCAATGATAACCGCTT+TGG | 0.409490 | 4.4:+17174383 | None:intergenic |
| TCTTCATCCATGTGATGTTA+TGG | 0.411069 | 4.4:-17174947 | MS.gene050117:CDS |
| TAACTTTACGCCTCTAAAAC+AGG | 0.412093 | 4.4:-17177935 | MS.gene050117:intron |
| CTTTGGATTTATCATCTTTG+CGG | 0.417763 | 4.4:+17174400 | None:intergenic |
| TCATATATAACTGGATAAAC+AGG | 0.422626 | 4.4:+17177496 | None:intergenic |
| AAATCCAAAGCGGTTATCAT+TGG | 0.422943 | 4.4:-17174387 | MS.gene050117:CDS |
| TTTACAAGGGAAAGCAAGTT+GGG | 0.428310 | 4.4:-17172313 | MS.gene050117:CDS |
| GGATCACCCAAAACCCATAA+AGG | 0.431211 | 4.4:-17173460 | MS.gene050117:intron |
| AGCTTCATCAAAATATTTGT+CGG | 0.433457 | 4.4:-17178775 | MS.gene050117:intron |
| CCTACTCTGCCTTGAAGGAT+TGG | 0.437874 | 4.4:-17179077 | MS.gene050117:CDS |
| TCTACAAAGGAGCAGGGATC+AGG | 0.438844 | 4.4:+17171961 | None:intergenic |
| TAAATTGTCTGACATTAGTA+AGG | 0.440874 | 4.4:-17179008 | MS.gene050117:CDS |
| CAAGCAATAATCGATAATAA+GGG | 0.444484 | 4.4:-17174781 | MS.gene050117:CDS |
| TGTCAGACAATTTATAAGTA+GGG | 0.445766 | 4.4:+17179017 | None:intergenic |
| TCTATCTTACATTACTGGAT+TGG | 0.449912 | 4.4:-17175771 | MS.gene050117:CDS |
| GAAATTCTTGCATATAATTG+AGG | 0.460329 | 4.4:-17177524 | MS.gene050117:CDS |
| GACAGAGTAATGGAAAAGAA+TGG | 0.463860 | 4.4:-17172545 | MS.gene050117:CDS |
| TCTTTACCACCAATTATCAA+TGG | 0.464631 | 4.4:-17176104 | MS.gene050117:CDS |
| CAGTTATATATGATAGTAAG+AGG | 0.467004 | 4.4:-17177486 | MS.gene050117:intron |
| GCTTAGCATGCAATTGCATC+CGG | 0.467826 | 4.4:+17175034 | None:intergenic |
| TTCTTAGGTAACAGCCTTCG+CGG | 0.469465 | 4.4:-17175897 | MS.gene050117:intron |
| AGGTTACCAAGTTCTTAAAC+CGG | 0.469844 | 4.4:-17175053 | MS.gene050117:CDS |
| ATGTCAGACAATTTATAAGT+AGG | 0.470269 | 4.4:+17179016 | None:intergenic |
| GGTTGCAATGGTCGGATTCA+CGG | 0.479021 | 4.4:-17174475 | MS.gene050117:CDS |
| TGGTGCTAATGCGGGATTTG+AGG | 0.479372 | 4.4:-17172855 | MS.gene050117:intron |
| CCCCAGGTCTTCAGACTTCG+AGG | 0.481586 | 4.4:-17174361 | MS.gene050117:intron |
| GTCGAAGTGATTTCTTCTGA+TGG | 0.483259 | 4.4:-17175842 | MS.gene050117:CDS |
| ATATTGTAGACGGATAAATC+AGG | 0.491517 | 4.4:+17175803 | None:intergenic |
| GGGCTTGAGCCAATCCTTCA+AGG | 0.493616 | 4.4:+17179068 | None:intergenic |
| GTTGAGTTCTACAAAGGAGC+AGG | 0.496069 | 4.4:+17171954 | None:intergenic |
| CGAAGAACTCTTGTCGCCAT+TGG | 0.496917 | 4.4:-17178178 | MS.gene050117:CDS |
| ATTTACAAGGGAAAGCAAGT+TGG | 0.498692 | 4.4:-17172314 | MS.gene050117:CDS |
| ATTGGTCGGAGTATCGTTAG+AGG | 0.505470 | 4.4:-17175753 | MS.gene050117:CDS |
| CTTCAGGGCTGATGAGCTCA+TGG | 0.507491 | 4.4:-17177758 | MS.gene050117:CDS |
| CTTGACACTACTTCCTCTGC+AGG | 0.508890 | 4.4:-17174737 | MS.gene050117:CDS |
| AACCTCGAAGTCTGAAGACC+TGG | 0.511782 | 4.4:+17174359 | None:intergenic |
| ATGGCAGGAAAGGAGCTTCA+GGG | 0.512062 | 4.4:-17177773 | MS.gene050117:intron |
| ATCATCTTTGCGGTTTAGCA+TGG | 0.515102 | 4.4:+17174410 | None:intergenic |
| TTTCTTAGAGCAGAGTATGA+AGG | 0.515713 | 4.4:+17174443 | None:intergenic |
| TCAGAAGAAATCACTTCGAC+AGG | 0.520858 | 4.4:+17175845 | None:intergenic |
| GGCGTAAAGTTAATACTAGA+AGG | 0.521759 | 4.4:+17177946 | None:intergenic |
| CTATACTGTGGTGCTAATGC+GGG | 0.524427 | 4.4:-17172863 | MS.gene050117:CDS |
| TGAAGAACATCACTTCTAGT+AGG | 0.530614 | 4.4:+17174964 | None:intergenic |
| AGGGGTGGGTACCTATTAGC+AGG | 0.533127 | 4.4:+17179957 | None:intergenic |
| ACAGAGTAATGGAAAAGAAT+GGG | 0.533786 | 4.4:-17172544 | MS.gene050117:CDS |
| GTTGTATGTGCTGTATTAAG+AGG | 0.534713 | 4.4:-17178848 | MS.gene050117:CDS |
| CATAATATATTCAGGCTGTA+CGG | 0.537286 | 4.4:-17173527 | MS.gene050117:intron |
| AAATTTCAGGTTGCAATGGT+CGG | 0.537370 | 4.4:-17174483 | MS.gene050117:intron |
| AGTATAGTGCAGCAAGTTGG+CGG | 0.538109 | 4.4:+17172879 | None:intergenic |
| GTTCTTTGTCTCCAGAGTGA+TGG | 0.544499 | 4.4:+17176070 | None:intergenic |
| TGGCTTGGTGGACAGAGTAA+TGG | 0.547034 | 4.4:-17172555 | MS.gene050117:CDS |
| CCTGGAACCCACAGAAAACT+CGG | 0.547117 | 4.4:+17179044 | None:intergenic |
| TTGTCTACCAGCAAGAAACT+CGG | 0.549695 | 4.4:+17172346 | None:intergenic |
| TTCAATGTTGAGTTCTACAA+AGG | 0.554393 | 4.4:+17171948 | None:intergenic |
| GAGCGATCTTATCAGAAAAG+AGG | 0.555737 | 4.4:-17174710 | MS.gene050117:intron |
| CAATTCCAAAACTGAAGCAT+AGG | 0.560611 | 4.4:+17180175 | None:intergenic |
| AATACAGCACATACAACATA+CGG | 0.563509 | 4.4:+17178854 | None:intergenic |
| ACCTCGAAGTCTGAAGACCT+GGG | 0.564197 | 4.4:+17174360 | None:intergenic |
| GCAGATAATTCATGGCTTGG+TGG | 0.572739 | 4.4:-17172567 | MS.gene050117:intron |
| TATGCAGATAATTCATGGCT+TGG | 0.573431 | 4.4:-17172570 | MS.gene050117:intron |
| CGGTTATCATTGGAAACCCC+AGG | 0.575178 | 4.4:-17174377 | MS.gene050117:CDS |
| ACAAGCTAGAATTATTTACA+AGG | 0.578446 | 4.4:-17172327 | MS.gene050117:CDS |
| TGCGCATTCAGCCATCACTC+TGG | 0.580971 | 4.4:-17176081 | MS.gene050117:CDS |
| ATCCAGATCATGAGTGCCAA+TGG | 0.581047 | 4.4:+17178162 | None:intergenic |
| CACAGTATAGTGCAGCAAGT+TGG | 0.583950 | 4.4:+17172876 | None:intergenic |
| AGAACAAGAAGAAGCTGATG+AGG | 0.587439 | 4.4:-17180009 | MS.gene050117:CDS |
| TCTTACATTACTGGATTGGT+CGG | 0.588320 | 4.4:-17175767 | MS.gene050117:CDS |
| AGTATCGTTAGAGGCAGAAG+AGG | 0.589218 | 4.4:-17175744 | MS.gene050117:intron |
| ACTATACTGTGGTGCTAATG+CGG | 0.590221 | 4.4:-17172864 | MS.gene050117:CDS |
| CAAGCTAGAATTATTTACAA+GGG | 0.593489 | 4.4:-17172326 | MS.gene050117:CDS |
| GTACGGACTAGTCTTATGCC+TGG | 0.599777 | 4.4:-17173510 | MS.gene050117:CDS |
| GAAAACTGTTGCTCACAACA+AGG | 0.602961 | 4.4:-17173481 | MS.gene050117:CDS |
| AAGCAATAATCGATAATAAG+GGG | 0.604733 | 4.4:-17174780 | MS.gene050117:CDS |
| TCATCCATGTGATGTTATGG+AGG | 0.610955 | 4.4:-17174944 | MS.gene050117:intron |
| TGTCTACCAGCAAGAAACTC+GGG | 0.611259 | 4.4:+17172347 | None:intergenic |
| CCAATCCTTCAAGGCAGAGT+AGG | 0.611521 | 4.4:+17179077 | None:intergenic |
| AGAAACTTCCATATTGTAGA+CGG | 0.611613 | 4.4:+17175793 | None:intergenic |
| TATTGTAGACGGATAAATCA+GGG | 0.617329 | 4.4:+17175804 | None:intergenic |
| TTGAAAGAAATCAACCTCGT+AGG | 0.620527 | 4.4:+17178120 | None:intergenic |
| AATCAGGATATTATATTGAG+CGG | 0.621082 | 4.4:-17172496 | MS.gene050117:CDS |
| AGCATTGAACCCATAAGCGA+TGG | 0.622930 | 4.4:+17174813 | None:intergenic |
| CTGCAGAGGAAGTAGTGTCA+AGG | 0.624219 | 4.4:+17174738 | None:intergenic |
| TCATGATCTGGATAAACTGG+AGG | 0.627307 | 4.4:-17178152 | MS.gene050117:CDS |
| TTTATCACCAGATGAAACAA+AGG | 0.630360 | 4.4:+17172517 | None:intergenic |
| AGAACATCACTTCTAGTAGG+AGG | 0.638419 | 4.4:+17174967 | None:intergenic |
| CACTGCTACTGATCTGACAA+AGG | 0.639733 | 4.4:-17176033 | MS.gene050117:CDS |
| TAAAATACATATAAAGCCAG+AGG | 0.642113 | 4.4:-17178975 | MS.gene050117:intron |
| TTGAGTTCTACAAAGGAGCA+GGG | 0.644199 | 4.4:+17171955 | None:intergenic |
| GAACCCACAGAAAACTCGGA+GGG | 0.649688 | 4.4:+17179048 | None:intergenic |
| CAACTTGCTGCACTATACTG+TGG | 0.650393 | 4.4:-17172875 | MS.gene050117:CDS |
| TGCGCACCATTGATAATTGG+TGG | 0.652799 | 4.4:+17176098 | None:intergenic |
| AGATCGCTCAGCTCCTGCAG+AGG | 0.653077 | 4.4:+17174724 | None:intergenic |
| AGTACCTCCATAACATCACA+TGG | 0.655899 | 4.4:+17174940 | None:intergenic |
| CATGATCTGGATAAACTGGA+GGG | 0.664709 | 4.4:-17178151 | MS.gene050117:CDS |
| GTAAAGTTAATACTAGAAGG+TGG | 0.672394 | 4.4:+17177949 | None:intergenic |
| CCTCGAAGTCTGAAGACCTG+GGG | 0.674003 | 4.4:+17174361 | None:intergenic |
| TGCAATGGTCGGATTCACGG+AGG | 0.701182 | 4.4:-17174472 | MS.gene050117:CDS |
| CAAAGATGATAAATCCAAAG+CGG | 0.701337 | 4.4:-17174397 | MS.gene050117:CDS |
| GGAACCCACAGAAAACTCGG+AGG | 0.726992 | 4.4:+17179047 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 17171936 | 17180216 | 17171936 | ID=MS.gene050117 |
| chr4.4 | mRNA | 17171936 | 17180216 | 17171936 | ID=MS.gene050117.t1;Parent=MS.gene050117 |
| chr4.4 | exon | 17180161 | 17180216 | 17180161 | ID=MS.gene050117.t1.exon1;Parent=MS.gene050117.t1 |
| chr4.4 | CDS | 17180161 | 17180216 | 17180161 | ID=cds.MS.gene050117.t1;Parent=MS.gene050117.t1 |
| chr4.4 | exon | 17179969 | 17180069 | 17179969 | ID=MS.gene050117.t1.exon2;Parent=MS.gene050117.t1 |
| chr4.4 | CDS | 17179969 | 17180069 | 17179969 | ID=cds.MS.gene050117.t1;Parent=MS.gene050117.t1 |
| chr4.4 | exon | 17178976 | 17179105 | 17178976 | ID=MS.gene050117.t1.exon3;Parent=MS.gene050117.t1 |
| chr4.4 | CDS | 17178976 | 17179105 | 17178976 | ID=cds.MS.gene050117.t1;Parent=MS.gene050117.t1 |
| chr4.4 | exon | 17178776 | 17178891 | 17178776 | ID=MS.gene050117.t1.exon4;Parent=MS.gene050117.t1 |
| chr4.4 | CDS | 17178776 | 17178891 | 17178776 | ID=cds.MS.gene050117.t1;Parent=MS.gene050117.t1 |
| chr4.4 | exon | 17178135 | 17178201 | 17178135 | ID=MS.gene050117.t1.exon5;Parent=MS.gene050117.t1 |
| chr4.4 | CDS | 17178135 | 17178201 | 17178135 | ID=cds.MS.gene050117.t1;Parent=MS.gene050117.t1 |
| chr4.4 | exon | 17177936 | 17177977 | 17177936 | ID=MS.gene050117.t1.exon6;Parent=MS.gene050117.t1 |
| chr4.4 | CDS | 17177936 | 17177977 | 17177936 | ID=cds.MS.gene050117.t1;Parent=MS.gene050117.t1 |
| chr4.4 | exon | 17177747 | 17177788 | 17177747 | ID=MS.gene050117.t1.exon7;Parent=MS.gene050117.t1 |
| chr4.4 | CDS | 17177747 | 17177788 | 17177747 | ID=cds.MS.gene050117.t1;Parent=MS.gene050117.t1 |
| chr4.4 | exon | 17177487 | 17177563 | 17177487 | ID=MS.gene050117.t1.exon8;Parent=MS.gene050117.t1 |
| chr4.4 | CDS | 17177487 | 17177563 | 17177487 | ID=cds.MS.gene050117.t1;Parent=MS.gene050117.t1 |
| chr4.4 | exon | 17176010 | 17176136 | 17176010 | ID=MS.gene050117.t1.exon9;Parent=MS.gene050117.t1 |
| chr4.4 | CDS | 17176010 | 17176136 | 17176010 | ID=cds.MS.gene050117.t1;Parent=MS.gene050117.t1 |
| chr4.4 | exon | 17175745 | 17175912 | 17175745 | ID=MS.gene050117.t1.exon10;Parent=MS.gene050117.t1 |
| chr4.4 | CDS | 17175745 | 17175912 | 17175745 | ID=cds.MS.gene050117.t1;Parent=MS.gene050117.t1 |
| chr4.4 | exon | 17174945 | 17175073 | 17174945 | ID=MS.gene050117.t1.exon11;Parent=MS.gene050117.t1 |
| chr4.4 | CDS | 17174945 | 17175073 | 17174945 | ID=cds.MS.gene050117.t1;Parent=MS.gene050117.t1 |
| chr4.4 | exon | 17174711 | 17174842 | 17174711 | ID=MS.gene050117.t1.exon12;Parent=MS.gene050117.t1 |
| chr4.4 | CDS | 17174711 | 17174842 | 17174711 | ID=cds.MS.gene050117.t1;Parent=MS.gene050117.t1 |
| chr4.4 | exon | 17174362 | 17174496 | 17174362 | ID=MS.gene050117.t1.exon13;Parent=MS.gene050117.t1 |
| chr4.4 | CDS | 17174362 | 17174496 | 17174362 | ID=cds.MS.gene050117.t1;Parent=MS.gene050117.t1 |
| chr4.4 | exon | 17173461 | 17173535 | 17173461 | ID=MS.gene050117.t1.exon14;Parent=MS.gene050117.t1 |
| chr4.4 | CDS | 17173461 | 17173535 | 17173461 | ID=cds.MS.gene050117.t1;Parent=MS.gene050117.t1 |
| chr4.4 | exon | 17172856 | 17172960 | 17172856 | ID=MS.gene050117.t1.exon15;Parent=MS.gene050117.t1 |
| chr4.4 | CDS | 17172856 | 17172960 | 17172856 | ID=cds.MS.gene050117.t1;Parent=MS.gene050117.t1 |
| chr4.4 | exon | 17172490 | 17172585 | 17172490 | ID=MS.gene050117.t1.exon16;Parent=MS.gene050117.t1 |
| chr4.4 | CDS | 17172490 | 17172585 | 17172490 | ID=cds.MS.gene050117.t1;Parent=MS.gene050117.t1 |
| chr4.4 | exon | 17172289 | 17172372 | 17172289 | ID=MS.gene050117.t1.exon17;Parent=MS.gene050117.t1 |
| chr4.4 | CDS | 17172289 | 17172372 | 17172289 | ID=cds.MS.gene050117.t1;Parent=MS.gene050117.t1 |
| chr4.4 | exon | 17171936 | 17172004 | 17171936 | ID=MS.gene050117.t1.exon18;Parent=MS.gene050117.t1 |
| chr4.4 | CDS | 17171936 | 17172004 | 17171936 | ID=cds.MS.gene050117.t1;Parent=MS.gene050117.t1 |
| Gene Sequence |
| Protein sequence |
