AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene08528

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08528.t1 MTR_8g024520 92.527 281 19 2 1 280 1 280 0.0 503
MS.gene08528.t1 MTR_8g024520 91.489 282 22 2 1 280 1 282 0.0 502
MS.gene08528.t1 MTR_3g116130 55.500 200 73 9 18 209 23 214 1.61e-55 181
MS.gene08528.t1 MTR_3g116130 55.500 200 73 9 18 209 23 214 1.65e-54 181
MS.gene08528.t1 MTR_4g035310 46.415 265 107 11 9 243 1 260 5.62e-50 166
MS.gene08528.t1 MTR_4g035310 55.233 172 64 3 9 170 1 169 1.08e-49 165
MS.gene08528.t1 MTR_3g092490 52.000 175 75 6 1 170 1 171 1.31e-47 159
MS.gene08528.t1 MTR_3g092490 54.194 155 65 4 1 153 1 151 1.82e-47 156
MS.gene08528.t1 MTR_3g092490 52.273 176 74 6 1 170 1 172 4.63e-47 158
MS.gene08528.t1 MTR_5g064120 75.862 87 21 0 15 101 7 93 8.76e-45 153
MS.gene08528.t1 MTR_7g117495 62.626 99 35 1 9 105 1 99 1.06e-40 141
MS.gene08528.t1 MTR_7g117495 62.626 99 35 1 9 105 1 99 1.39e-40 141
MS.gene08528.t1 MTR_4g088010 48.810 84 43 0 20 103 72 155 4.99e-19 85.9
MS.gene08528.t1 MTR_2g026180 36.066 122 74 2 25 143 5 125 7.07e-19 86.3
MS.gene08528.t1 MTR_8g463890 42.857 98 56 0 5 102 54 151 8.19e-19 85.9
MS.gene08528.t1 MTR_3g055990 39.506 81 48 1 20 100 2 81 7.35e-17 80.5
MS.gene08528.t1 MTR_3g055990 39.506 81 48 1 20 100 2 81 7.54e-17 80.5
MS.gene08528.t1 MTR_5g097580 36.066 122 72 3 25 143 7 125 2.77e-16 79.0
MS.gene08528.t1 MTR_5g097580 36.066 122 72 3 25 143 7 125 3.08e-16 78.6
MS.gene08528.t1 MTR_7g088140 42.169 83 48 0 20 102 123 205 1.82e-15 76.3
MS.gene08528.t1 MTR_7g088140 42.169 83 48 0 20 102 123 205 3.18e-15 75.9
MS.gene08528.t1 MTR_5g088220 40.964 83 49 0 20 102 161 243 4.56e-15 75.1
MS.gene08528.t1 MTR_4g070190 43.421 76 42 1 25 99 9 84 1.77e-14 70.1
MS.gene08528.t1 MTR_4g070080 43.590 78 39 2 25 99 7 82 4.01e-14 68.2
MS.gene08528.t1 MTR_1g073690 36.250 80 50 1 20 99 2 80 4.71e-14 72.0
MS.gene08528.t1 MTR_3g084040 40.580 69 41 0 24 92 36 104 8.12e-14 67.8
MS.gene08528.t1 MTR_4g070080 43.590 78 39 2 25 99 7 82 8.97e-14 68.2
MS.gene08528.t1 MTR_3g084040 40.909 66 39 0 24 89 36 101 1.98e-13 65.5
MS.gene08528.t1 MTR_4g109540 41.558 77 44 1 24 99 42 118 2.02e-13 68.9
MS.gene08528.t1 MTR_7g013730 38.961 77 46 1 25 100 41 117 3.57e-13 68.2
MS.gene08528.t1 MTR_4g070140 43.421 76 42 1 25 99 9 84 3.70e-13 67.0
MS.gene08528.t1 MTR_4g063560 45.070 71 38 1 24 93 33 103 4.15e-13 64.7
MS.gene08528.t1 MTR_3g025880 39.506 81 40 2 24 99 44 120 8.41e-13 68.6
MS.gene08528.t1 MTR_7g105260 33.333 99 62 2 25 122 33 128 9.01e-12 62.0
MS.gene08528.t1 MTR_7g013740 41.026 78 41 2 25 99 41 116 2.08e-11 61.2
MS.gene08528.t1 MTR_3g080130 34.783 69 41 1 24 92 36 100 2.29e-11 60.8
MS.gene08528.t1 MTR_3g082650 40.909 66 39 0 25 90 19 84 3.55e-11 63.2
MS.gene08528.t1 MTR_4g109540 37.647 85 44 2 24 99 42 126 5.06e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08528.t1 AT2G46780 56.420 257 73 11 1 223 1 252 7.00e-77 237
MS.gene08528.t1 AT2G46780 56.420 257 73 11 1 223 1 252 7.00e-77 237
MS.gene08528.t1 AT1G33470 55.063 158 64 4 19 170 2 158 2.07e-52 171
MS.gene08528.t1 AT1G33470 54.717 159 64 5 19 170 2 159 9.20e-51 167
MS.gene08528.t1 AT1G33470 54.717 159 64 5 19 170 2 159 1.38e-50 167
MS.gene08528.t1 AT1G20880 49.758 207 80 8 18 209 18 215 1.91e-48 162
MS.gene08528.t1 AT1G20880 49.758 207 80 8 18 209 18 215 1.91e-48 162
MS.gene08528.t1 AT1G20880 49.758 207 80 8 18 209 18 215 1.91e-48 162
MS.gene08528.t1 AT1G76460 41.509 265 116 10 18 266 18 259 5.75e-48 161
MS.gene08528.t1 AT1G22910 51.266 158 71 3 20 171 9 166 5.77e-44 149
MS.gene08528.t1 AT1G22910 51.266 158 71 3 20 171 9 166 9.43e-44 149
MS.gene08528.t1 AT1G22910 51.266 158 71 3 20 171 9 166 4.73e-43 150
MS.gene08528.t1 AT1G22330 70.588 85 25 0 18 102 11 95 1.82e-39 139
MS.gene08528.t1 AT1G78260 70.238 84 25 0 18 101 11 94 4.43e-39 138
MS.gene08528.t1 AT1G78260 70.238 84 25 0 18 101 11 94 1.24e-38 137
MS.gene08528.t1 AT3G54770 49.231 130 65 1 9 137 1 130 3.44e-38 135
MS.gene08528.t1 AT3G54770 49.231 130 65 1 9 137 1 130 4.92e-38 135
MS.gene08528.t1 AT5G11412 63.855 83 30 0 20 102 48 130 1.91e-34 123
MS.gene08528.t1 AT5G53680 57.143 84 36 0 20 103 9 92 1.36e-30 113
MS.gene08528.t1 AT3G06970 49.558 113 53 2 20 129 8 119 1.83e-30 116
MS.gene08528.t1 AT5G53720 57.500 80 34 0 24 103 9 88 5.59e-26 99.0
MS.gene08528.t1 AT3G54770 50.000 98 38 1 40 137 1 87 3.01e-25 100
MS.gene08528.t1 AT2G22090 49.398 83 42 0 20 102 100 182 4.91e-19 85.9
MS.gene08528.t1 AT2G22090 49.398 83 42 0 20 102 100 182 5.37e-19 85.9
MS.gene08528.t1 AT3G15010 40.816 98 58 0 5 102 56 153 2.10e-17 81.6
MS.gene08528.t1 AT3G15010 40.816 98 58 0 5 102 56 153 2.10e-17 81.6
MS.gene08528.t1 AT5G47620 44.737 76 41 1 25 100 7 81 2.19e-17 82.0
MS.gene08528.t1 AT5G47620 44.737 76 41 1 25 100 7 81 2.19e-17 82.0
MS.gene08528.t1 AT5G47620 44.737 76 41 1 25 100 7 81 2.19e-17 82.0
MS.gene08528.t1 AT5G47620 48.529 68 34 1 25 92 7 73 2.61e-17 81.6
MS.gene08528.t1 AT4G26650 37.500 80 49 1 21 100 9 87 2.18e-16 79.0
MS.gene08528.t1 AT4G26650 37.500 80 49 1 21 100 12 90 2.33e-16 79.0
MS.gene08528.t1 AT5G55550 39.726 73 43 1 20 92 2 73 2.51e-16 79.0
MS.gene08528.t1 AT5G55550 39.726 73 43 1 20 92 2 73 2.51e-16 79.0
MS.gene08528.t1 AT5G55550 39.726 73 43 1 20 92 2 73 2.51e-16 79.0
MS.gene08528.t1 AT5G55550 39.726 73 43 1 20 92 2 73 2.51e-16 79.0
MS.gene08528.t1 AT5G55550 39.726 73 43 1 20 92 2 73 2.51e-16 79.0
MS.gene08528.t1 AT5G55550 39.726 73 43 1 20 92 2 73 2.54e-16 79.0
MS.gene08528.t1 AT5G55550 39.726 73 43 1 20 92 2 73 2.54e-16 79.0
MS.gene08528.t1 AT5G55550 39.726 73 43 1 20 92 2 73 2.54e-16 79.0
MS.gene08528.t1 AT5G55550 39.726 73 43 1 20 92 2 73 2.54e-16 79.0
MS.gene08528.t1 AT5G55550 39.726 73 43 1 20 92 2 73 2.62e-16 79.0
MS.gene08528.t1 AT5G55550 39.726 73 43 1 20 92 2 73 2.65e-16 79.0
MS.gene08528.t1 AT3G07810 43.038 79 38 2 25 100 7 81 9.72e-16 77.4
MS.gene08528.t1 AT3G07810 43.038 79 38 2 25 100 7 81 1.00e-15 77.4
MS.gene08528.t1 AT4G13850 44.444 72 36 1 24 95 35 102 1.56e-15 72.0
MS.gene08528.t1 AT4G13850 44.444 72 36 1 24 95 35 102 2.35e-15 72.0
MS.gene08528.t1 AT4G39260 43.421 76 42 1 25 99 7 82 2.77e-15 71.2
MS.gene08528.t1 AT4G13850 44.444 72 36 1 24 95 35 102 2.88e-15 72.0
MS.gene08528.t1 AT4G13850 44.444 72 36 1 24 95 35 102 3.22e-15 72.0
MS.gene08528.t1 AT2G22100 44.048 84 43 1 20 103 159 238 3.87e-15 75.1
MS.gene08528.t1 AT3G23830 41.558 77 44 1 24 99 35 111 4.06e-15 71.2
MS.gene08528.t1 AT3G23830 41.558 77 44 1 24 99 35 111 4.06e-15 71.2
MS.gene08528.t1 AT4G14300 40.741 81 47 1 20 100 2 81 4.22e-15 75.1
MS.gene08528.t1 AT4G14300 40.741 81 47 1 20 100 2 81 4.22e-15 75.1
MS.gene08528.t1 AT4G39260 43.421 76 42 1 25 99 7 82 4.39e-15 69.7
MS.gene08528.t1 AT1G17640 38.824 85 51 1 25 109 67 150 4.65e-15 74.7
MS.gene08528.t1 AT1G17640 38.824 85 51 1 25 109 67 150 4.65e-15 74.7
MS.gene08528.t1 AT1G17640 38.824 85 51 1 25 109 67 150 4.65e-15 74.7
MS.gene08528.t1 AT2G41060 39.759 83 50 0 20 102 124 206 7.11e-15 74.7
MS.gene08528.t1 AT2G41060 39.759 83 50 0 20 102 124 206 7.11e-15 74.7
MS.gene08528.t1 AT2G41060 39.759 83 50 0 20 102 124 206 7.11e-15 74.7
MS.gene08528.t1 AT4G39260 43.421 76 42 1 25 99 7 82 7.33e-15 71.2
MS.gene08528.t1 AT3G56860 40.964 83 49 0 20 102 136 218 1.08e-14 74.3
MS.gene08528.t1 AT3G56860 40.964 83 49 0 20 102 136 218 1.08e-14 74.3
MS.gene08528.t1 AT3G56860 40.964 83 49 0 20 102 136 218 1.08e-14 74.3
MS.gene08528.t1 AT3G56860 40.964 83 49 0 20 102 136 218 1.08e-14 74.3
MS.gene08528.t1 AT3G56860 40.964 83 49 0 20 102 136 218 1.08e-14 74.3
MS.gene08528.t1 AT3G56860 40.964 83 49 0 20 102 136 218 1.08e-14 74.3
MS.gene08528.t1 AT3G56860 40.964 83 49 0 20 102 136 218 1.08e-14 74.3
MS.gene08528.t1 AT3G56860 40.964 83 49 0 20 102 136 218 1.08e-14 74.3
MS.gene08528.t1 AT3G56860 40.964 83 49 0 20 102 136 218 1.08e-14 74.3
MS.gene08528.t1 AT3G56860 40.964 83 49 0 20 102 136 218 1.08e-14 74.3
MS.gene08528.t1 AT3G56860 40.964 83 49 0 20 102 136 218 1.08e-14 74.3
MS.gene08528.t1 AT2G19380 43.750 80 45 0 20 99 404 483 1.17e-14 74.3
MS.gene08528.t1 AT4G39260 47.458 59 31 0 25 83 7 65 1.97e-14 68.2
MS.gene08528.t1 AT2G33410 40.789 76 44 1 25 100 7 81 2.47e-14 72.8
MS.gene08528.t1 AT2G21660 42.105 76 43 1 25 99 9 84 2.52e-14 69.3
MS.gene08528.t1 AT2G21660 42.105 76 43 1 25 99 9 84 3.57e-14 69.3
MS.gene08528.t1 AT5G61030 43.478 69 35 1 24 92 40 104 1.91e-13 69.7
MS.gene08528.t1 AT5G61030 43.478 69 35 1 24 92 40 104 1.91e-13 69.7
MS.gene08528.t1 AT1G74230 42.105 76 44 0 24 99 34 109 2.67e-13 68.9
MS.gene08528.t1 AT1G18630 39.785 93 49 3 5 92 15 105 4.32e-13 65.9
MS.gene08528.t1 AT3G26420 45.614 57 31 0 25 81 8 64 5.07e-13 67.8
MS.gene08528.t1 AT5G59865 51.020 49 24 0 24 72 26 74 2.21e-11 60.1
MS.gene08528.t1 AT1G58470 39.189 74 41 1 25 94 7 80 2.84e-11 63.5
MS.gene08528.t1 AT1G58470 39.189 74 41 1 25 94 7 80 2.84e-11 63.5

Find 80 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCCTTCTGTTGGAGCTTC+TGG 0.055135 8.3:-67938538 MS.gene08528:CDS
GCTGAGGCAAAATTGGAATT+TGG 0.157884 8.3:+67938419 None:intergenic
CATAGTAAGGGTACATATTT+TGG 0.172442 8.3:+67938500 None:intergenic
TAACCCTCCAACAAATATTT+TGG 0.186878 8.3:+67940065 None:intergenic
CCAAAATGCTGAGGCAAAAT+TGG 0.204224 8.3:+67938412 None:intergenic
TATTTATCTGACAGATATTC+TGG 0.249082 8.3:-67939109 MS.gene08528:intron
AATTGGAATTTGGGTCATTT+GGG 0.251882 8.3:+67938429 None:intergenic
AGTATCCATGGTCATGGTTT+TGG 0.271120 8.3:-67938463 MS.gene08528:CDS
AGCTTCTGGGGCAATGGGTT+TGG 0.272297 8.3:-67938524 MS.gene08528:CDS
AAATTGGAATTTGGGTCATT+TGG 0.281074 8.3:+67938428 None:intergenic
ACATTTCCTTATTCAGCATA+TGG 0.317744 8.3:-67939232 MS.gene08528:CDS
ATCCTTCTGTTGGAGCTTCT+GGG 0.321902 8.3:-67938537 MS.gene08528:CDS
AGTTACCCATATGCTGAATA+AGG 0.334120 8.3:+67939226 None:intergenic
GTTATCACTGATAAGTATAC+TGG 0.334320 8.3:-67939973 MS.gene08528:CDS
TGTTGGAGCTTCTGGGGCAA+TGG 0.334769 8.3:-67938530 MS.gene08528:CDS
GCTCTTCTTCCATCAATAAC+TGG 0.337494 8.3:+67939747 None:intergenic
CAGAACTATTACAATGTCTA+TGG 0.345598 8.3:-67938583 MS.gene08528:CDS
AGAATTATTACCAGCACTAA+TGG 0.360267 8.3:+67938345 None:intergenic
CTGAGGCAAAATTGGAATTT+GGG 0.360464 8.3:+67938420 None:intergenic
CAAAATATGTACCCTTACTA+TGG 0.370998 8.3:-67938499 MS.gene08528:CDS
GTTGGAGCTTCTGGGGCAAT+GGG 0.372650 8.3:-67938529 MS.gene08528:CDS
AATGGTGTGCAGGAAGGTTT+AGG 0.381511 8.3:-67939333 MS.gene08528:intron
ACTGGGAGATCTAAAGGTTA+TGG 0.382688 8.3:-67939955 MS.gene08528:CDS
TAAATGTTGTGTCATTATAT+TGG 0.402817 8.3:+67940088 None:intergenic
ACATTTACCAAAATATTTGT+TGG 0.412578 8.3:-67940072 MS.gene08528:CDS
AATCCATCTCCAGTTATTGA+TGG 0.412922 8.3:-67939756 MS.gene08528:CDS
TATTTGTTGGAGGGTTAGCT+TGG 0.423354 8.3:-67940059 MS.gene08528:CDS
TATTACCAGCACTAATGGTT+GGG 0.433671 8.3:+67938350 None:intergenic
TGTGCATATTGACCATAGTA+AGG 0.433964 8.3:+67938487 None:intergenic
CATTTCCTTATTCAGCATAT+GGG 0.440818 8.3:-67939231 MS.gene08528:intron
TGTACTGCCTAGTGGGTTGA+TGG 0.442879 8.3:+67939254 None:intergenic
TTGTTACCACTCACCATATG+AGG 0.446616 8.3:+67940117 None:intergenic
ATTTGTTGGAGGGTTAGCTT+GGG 0.450733 8.3:-67940058 MS.gene08528:CDS
GTACAATCAGTTCCTTATCA+TGG 0.451060 8.3:-67939295 MS.gene08528:CDS
TCAACAAAGACAACCTCATA+TGG 0.453555 8.3:-67940130 MS.gene08528:CDS
GCCGGTCCTTCACAAGCCTC+TGG 0.455977 8.3:-67937903 MS.gene08528:CDS
TCACCTTACTATCCTTCTGT+TGG 0.471067 8.3:-67938547 MS.gene08528:CDS
GCTCCAACAGAAGGATAGTA+AGG 0.482209 8.3:+67938544 None:intergenic
CATGCTCTTATAGCTGCCTC+TGG 0.486338 8.3:+67939783 None:intergenic
AAGTATACTGGGAGATCTAA+AGG 0.490906 8.3:-67939961 MS.gene08528:CDS
TCCAGAGGCTTGTGAAGGAC+CGG 0.491823 8.3:+67937902 None:intergenic
AATTGCAATATTGCTTGTCT+TGG 0.495441 8.3:-67939723 MS.gene08528:CDS
CTTCCATCAATAACTGGAGA+TGG 0.499114 8.3:+67939753 None:intergenic
TTATTACCAGCACTAATGGT+TGG 0.519138 8.3:+67938349 None:intergenic
TTTACCAAAATATTTGTTGG+AGG 0.522261 8.3:-67940069 MS.gene08528:CDS
TCCTTCTGTTGGAGCTTCTG+GGG 0.522825 8.3:-67938536 MS.gene08528:CDS
TTGTTCAGTATTTGTTCCAG+AGG 0.523327 8.3:+67937887 None:intergenic
TTGGAATTTGGGTCATTTGG+GGG 0.536181 8.3:+67938431 None:intergenic
GGTTAGCTTGGGAAACTCAA+AGG 0.538530 8.3:-67940047 MS.gene08528:CDS
AGGAAATGTGTACTGCCTAG+TGG 0.539088 8.3:+67939246 None:intergenic
TGCTGCTATTGTAGTAGTTG+TGG 0.540042 8.3:+67937944 None:intergenic
GTGGATGAAGAACCATGATA+AGG 0.546300 8.3:+67939283 None:intergenic
TTACCAAAATATTTGTTGGA+GGG 0.548943 8.3:-67940068 MS.gene08528:CDS
GTTGGGATTGCAACTGAAGA+AGG 0.551412 8.3:+67938367 None:intergenic
CAATATGCACAAAGTATCCA+TGG 0.551625 8.3:-67938475 MS.gene08528:CDS
GCAATCCCAACCATTAGTGC+TGG 0.558559 8.3:-67938355 MS.gene08528:intron
CCGGCCCTTGTTACTACCAC+AGG 0.562277 8.3:+67937921 None:intergenic
GCCCCAGAAGCTCCAACAGA+AGG 0.575329 8.3:+67938535 None:intergenic
ATTGGAATTTGGGTCATTTG+GGG 0.578017 8.3:+67938430 None:intergenic
GCACAAAGTATCCATGGTCA+TGG 0.581950 8.3:-67938469 MS.gene08528:CDS
CAGCACCTGTGGTAGTAACA+AGG 0.583493 8.3:-67937926 MS.gene08528:CDS
TTTGTTCCAGAGGCTTGTGA+AGG 0.591071 8.3:+67937897 None:intergenic
GTTAGCTTGGGAAACTCAAA+GGG 0.597037 8.3:-67940046 MS.gene08528:CDS
GTGCATATTGACCATAGTAA+GGG 0.598572 8.3:+67938488 None:intergenic
TACTACAATAGCAGCACCTG+TGG 0.606819 8.3:-67937937 MS.gene08528:CDS
TTATCACTGATAAGTATACT+GGG 0.625319 8.3:-67939972 MS.gene08528:CDS
CGATCACTAACTCTTCAGCA+TGG 0.635608 8.3:-67939687 MS.gene08528:intron
GAACTCCAAAACCATGACCA+TGG 0.639031 8.3:+67938458 None:intergenic
CCTGTGGTAGTAACAAGGGC+CGG 0.642231 8.3:-67937921 MS.gene08528:CDS
GGAAGGTTTAGGCCTCCACA+TGG 0.645450 8.3:-67939322 MS.gene08528:CDS
GGAAATGTGTACTGCCTAGT+GGG 0.657310 8.3:+67939247 None:intergenic
AGACAACCTCATATGGTGAG+TGG 0.661054 8.3:-67940123 MS.gene08528:CDS
AGCACCTGTGGTAGTAACAA+GGG 0.663200 8.3:-67937925 MS.gene08528:CDS
CCTTGTGACCCAAAATGCTG+AGG 0.666097 8.3:+67938403 None:intergenic
GTTTAGGCCTCCACATGGAG+TGG 0.666440 8.3:-67939317 MS.gene08528:CDS
GGTGACATTTAAGGATCCAG+AGG 0.676171 8.3:-67939799 MS.gene08528:CDS
GATTGTACCACTCCATGTGG+AGG 0.680340 8.3:+67939310 None:intergenic
ACTGATTGTACCACTCCATG+TGG 0.705430 8.3:+67939307 None:intergenic
ACAAGACACATTGTATCCTG+CGG 0.715658 8.3:-67939080 MS.gene08528:intron
AGTGGGTTGATGGAAAACTG+TGG 0.742505 8.3:+67939264 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTAAAAAAAAAA+AGG - chr8.3:67938426-67938445 MS.gene08528:CDS 0.0%
!!! TTTTTTTTTTTAAAAAAAAA+AGG + chr8.3:67938427-67938446 None:intergenic 0.0%
!! TAGTTTCTTTAATAAAAAAA+AGG + chr8.3:67938537-67938556 None:intergenic 10.0%
!!! ATTTTTTTTTTTGTTTTTGT+AGG - chr8.3:67938184-67938203 MS.gene08528:intron 10.0%
!!! TATGTTTTATAAGATTTTAA+AGG - chr8.3:67938639-67938658 MS.gene08528:intron 10.0%
!! AAATATAAGGAAAAGAAAAA+TGG + chr8.3:67938085-67938104 None:intergenic 15.0%
!! AGCATAGTAAAATAAATATA+AGG + chr8.3:67938098-67938117 None:intergenic 15.0%
!! CAAATTAATCACATATATAT+TGG + chr8.3:67939224-67939243 None:intergenic 15.0%
!! TTTAATCTCTCAAATTATAA+AGG + chr8.3:67939968-67939987 None:intergenic 15.0%
!! TTTCTTTAATAAAAAAAAGG+TGG + chr8.3:67938534-67938553 None:intergenic 15.0%
!!! AGAATTTATTTTGAACAATT+TGG - chr8.3:67937989-67938008 MS.gene08528:intron 15.0%
!!! TTTTAAAGGAAAAATTGAAA+TGG - chr8.3:67938653-67938672 MS.gene08528:intron 15.0%
!! AAATAATCAACCATAAAAAG+TGG + chr8.3:67938560-67938579 None:intergenic 20.0%
!! AAGAAAGAAATAAAAGAGAA+TGG + chr8.3:67938357-67938376 None:intergenic 20.0%
!! ACATTTACCAAAATATTTGT+TGG - chr8.3:67937932-67937951 MS.gene08528:CDS 20.0%
!! GTTTCAATTAATAGTGAAAT+CGG - chr8.3:67938974-67938993 MS.gene08528:intron 20.0%
!! TTAAAGAATCAACTAAATCA+CGG - chr8.3:67939286-67939305 MS.gene08528:CDS 20.0%
!!! GGTATATTGTGTTATTTATT+TGG - chr8.3:67938447-67938466 MS.gene08528:CDS 20.0%
!!! TAAATGTTGTGTCATTATAT+TGG + chr8.3:67937919-67937938 None:intergenic 20.0%
!!! TAATTTTGTCAAAATCATGT+TGG - chr8.3:67939166-67939185 MS.gene08528:intron 20.0%
! AAATAAAAGAGAATGGTAGA+AGG + chr8.3:67938350-67938369 None:intergenic 25.0%
! TAGAATTGACATCAAAGAAA+AGG + chr8.3:67938872-67938891 None:intergenic 25.0%
! TATTTATCTGACAGATATTC+TGG - chr8.3:67938895-67938914 MS.gene08528:intron 25.0%
! TCACTATTAATTGAAACATG+TGG + chr8.3:67938971-67938990 None:intergenic 25.0%
! TGAGCAATTCTATTTCAAAA+TGG - chr8.3:67939730-67939749 MS.gene08528:CDS 25.0%
! TTATCACTGATAAGTATACT+GGG - chr8.3:67938032-67938051 MS.gene08528:intron 25.0%
! TTCATTATGTATTCGTTGTA+AGG - chr8.3:67939799-67939818 MS.gene08528:CDS 25.0%
!! TTACCAAAATATTTGTTGGA+GGG - chr8.3:67937936-67937955 MS.gene08528:CDS 25.0%
!! TTTACCAAAATATTTGTTGG+AGG - chr8.3:67937935-67937954 MS.gene08528:CDS 25.0%
!!! AAAGAAACTACCACTTTTTA+TGG - chr8.3:67938547-67938566 MS.gene08528:CDS 25.0%
!!! TGATCATATTAGTCTTTTCA+GGG + chr8.3:67939883-67939902 None:intergenic 25.0%
!!! TTGATCATATTAGTCTTTTC+AGG + chr8.3:67939884-67939903 None:intergenic 25.0%
AAATTGGAATTTGGGTCATT+TGG + chr8.3:67939579-67939598 None:intergenic 30.0%
AATCACGATGACACAATAAT+GGG - chr8.3:67939000-67939019 MS.gene08528:intron 30.0%
AATTGCAATATTGCTTGTCT+TGG - chr8.3:67938281-67938300 MS.gene08528:intron 30.0%
AATTGGAATTTGGGTCATTT+GGG + chr8.3:67939578-67939597 None:intergenic 30.0%
ACATTTCCTTATTCAGCATA+TGG - chr8.3:67938772-67938791 MS.gene08528:intron 30.0%
AGAATTATTACCAGCACTAA+TGG + chr8.3:67939662-67939681 None:intergenic 30.0%
CAAAATATGTACCCTTACTA+TGG - chr8.3:67939505-67939524 MS.gene08528:intron 30.0%
CAAAATTATGAGTAGCACTT+TGG + chr8.3:67939154-67939173 None:intergenic 30.0%
CAGAACTATTACAATGTCTA+TGG - chr8.3:67939421-67939440 MS.gene08528:intron 30.0%
CATTTCCTTATTCAGCATAT+GGG - chr8.3:67938773-67938792 MS.gene08528:intron 30.0%
CTAGGATGAATCAAATTGAT+AGG + chr8.3:67938500-67938519 None:intergenic 30.0%
CTATCAATTTGATTCATCCT+AGG - chr8.3:67938498-67938517 MS.gene08528:CDS 30.0%
GTTATCACTGATAAGTATAC+TGG - chr8.3:67938031-67938050 MS.gene08528:intron 30.0%
! ATACTTCCATGACATAGTTT+TGG - chr8.3:67939025-67939044 MS.gene08528:intron 30.0%
! CAAATTGATAGGTTGAACAA+TGG + chr8.3:67938489-67938508 None:intergenic 30.0%
! GTTTTTGTAGGTGACATTTA+AGG - chr8.3:67938196-67938215 MS.gene08528:intron 30.0%
! TAACCCTCCAACAAATATTT+TGG + chr8.3:67937942-67937961 None:intergenic 30.0%
!! CATAGTAAGGGTACATATTT+TGG + chr8.3:67939507-67939526 None:intergenic 30.0%
!!! ATCATATTAGTCTTTTCAGG+GGG + chr8.3:67939881-67939900 None:intergenic 30.0%
!!! GATCATATTAGTCTTTTCAG+GGG + chr8.3:67939882-67939901 None:intergenic 30.0%
AAGTATACTGGGAGATCTAA+AGG - chr8.3:67938043-67938062 MS.gene08528:intron 35.0%
AATCCATCTCCAGTTATTGA+TGG - chr8.3:67938248-67938267 MS.gene08528:intron 35.0%
AGTTACCCATATGCTGAATA+AGG + chr8.3:67938781-67938800 None:intergenic 35.0%
ATGACACCAAAACTATGTCA+TGG + chr8.3:67939034-67939053 None:intergenic 35.0%
ATTGGAATTTGGGTCATTTG+GGG + chr8.3:67939577-67939596 None:intergenic 35.0%
CTGAGGCAAAATTGGAATTT+GGG + chr8.3:67939587-67939606 None:intergenic 35.0%
GAAAAATTGAAATGGTGTGC+AGG - chr8.3:67938661-67938680 MS.gene08528:intron 35.0%
GAATCACGATGACACAATAA+TGG - chr8.3:67938999-67939018 MS.gene08528:intron 35.0%
GTACAATCAGTTCCTTATCA+TGG - chr8.3:67938709-67938728 MS.gene08528:intron 35.0%
GTGCATATTGACCATAGTAA+GGG + chr8.3:67939519-67939538 None:intergenic 35.0%
TATTACCAGCACTAATGGTT+GGG + chr8.3:67939657-67939676 None:intergenic 35.0%
TCAACAAAGACAACCTCATA+TGG - chr8.3:67937874-67937893 MS.gene08528:CDS 35.0%
TGTGCATATTGACCATAGTA+AGG + chr8.3:67939520-67939539 None:intergenic 35.0%
TTATTACCAGCACTAATGGT+TGG + chr8.3:67939658-67939677 None:intergenic 35.0%
TTGTTCAGTATTTGTTCCAG+AGG + chr8.3:67940120-67940139 None:intergenic 35.0%
! AATGGCCAGAATTTTCCTAA+TGG + chr8.3:67939062-67939081 None:intergenic 35.0%
! CAATATGCACAAAGTATCCA+TGG - chr8.3:67939529-67939548 MS.gene08528:intron 35.0%
! CAATTTTCAGCAACAGTAAC+TGG - chr8.3:67940035-67940054 MS.gene08528:CDS 35.0%
! CAATTTTGCCTCAGCATTTT+GGG - chr8.3:67939593-67939612 MS.gene08528:intron 35.0%
!!! TCATATTAGTCTTTTCAGGG+GGG + chr8.3:67939880-67939899 None:intergenic 35.0%
!!! TGACATAGTTTTGGTGTCAT+TGG - chr8.3:67939034-67939053 MS.gene08528:intron 35.0%
AATTGAAATGGTGTGCAGGA+AGG - chr8.3:67938665-67938684 MS.gene08528:intron 40.0%
ACAAGACACATTGTATCCTG+CGG - chr8.3:67938924-67938943 MS.gene08528:intron 40.0%
ACACATCACACACACATCAA+TGG + chr8.3:67939080-67939099 None:intergenic 40.0%
ACTGGGAGATCTAAAGGTTA+TGG - chr8.3:67938049-67938068 MS.gene08528:intron 40.0%
ATTACATCAACACTTACCGC+AGG + chr8.3:67938943-67938962 None:intergenic 40.0%
CCAAAATGCTGAGGCAAAAT+TGG + chr8.3:67939595-67939614 None:intergenic 40.0%
CTTCCATCAATAACTGGAGA+TGG + chr8.3:67938254-67938273 None:intergenic 40.0%
GCTCTTCTTCCATCAATAAC+TGG + chr8.3:67938260-67938279 None:intergenic 40.0%
GCTGAGGCAAAATTGGAATT+TGG + chr8.3:67939588-67939607 None:intergenic 40.0%
GTGGATGAAGAACCATGATA+AGG + chr8.3:67938724-67938743 None:intergenic 40.0%
TCACCTTACTATCCTTCTGT+TGG - chr8.3:67939457-67939476 MS.gene08528:intron 40.0%
TGCTGCTATTGTAGTAGTTG+TGG + chr8.3:67940063-67940082 None:intergenic 40.0%
TTGGAATTTGGGTCATTTGG+GGG + chr8.3:67939576-67939595 None:intergenic 40.0%
TTGTTACCACTCACCATATG+AGG + chr8.3:67937890-67937909 None:intergenic 40.0%
! AGTATCCATGGTCATGGTTT+TGG - chr8.3:67939541-67939560 MS.gene08528:intron 40.0%
! CCAATTTTGCCTCAGCATTT+TGG - chr8.3:67939592-67939611 MS.gene08528:intron 40.0%
! GTTAGCTTGGGAAACTCAAA+GGG - chr8.3:67937958-67937977 MS.gene08528:intron 40.0%
! TGATAGGTTGAACAATGGCA+GGG + chr8.3:67938484-67938503 None:intergenic 40.0%
! TTGATAGGTTGAACAATGGC+AGG + chr8.3:67938485-67938504 None:intergenic 40.0%
!! ATTTGTTGGAGGGTTAGCTT+GGG - chr8.3:67937946-67937965 MS.gene08528:CDS 40.0%
!! TATTTGTTGGAGGGTTAGCT+TGG - chr8.3:67937945-67937964 MS.gene08528:CDS 40.0%
!! TGGTGCCATTAGGAAAATTC+TGG - chr8.3:67939054-67939073 MS.gene08528:intron 40.0%
AATGGTGTGCAGGAAGGTTT+AGG - chr8.3:67938671-67938690 MS.gene08528:intron 45.0%
ACTGATTGTACCACTCCATG+TGG + chr8.3:67938700-67938719 None:intergenic 45.0%
AGACAACCTCATATGGTGAG+TGG - chr8.3:67937881-67937900 MS.gene08528:CDS 45.0%
AGCACCTGTGGTAGTAACAA+GGG - chr8.3:67940079-67940098 MS.gene08528:CDS 45.0%
AGGAAATGTGTACTGCCTAG+TGG + chr8.3:67938761-67938780 None:intergenic 45.0%
ATCCTTCTGTTGGAGCTTCT+GGG - chr8.3:67939467-67939486 MS.gene08528:intron 45.0%
CGATCACTAACTCTTCAGCA+TGG - chr8.3:67938317-67938336 MS.gene08528:intron 45.0%
GAACTCCAAAACCATGACCA+TGG + chr8.3:67939549-67939568 None:intergenic 45.0%
GCTCCAACAGAAGGATAGTA+AGG + chr8.3:67939463-67939482 None:intergenic 45.0%
GGAAATGTGTACTGCCTAGT+GGG + chr8.3:67938760-67938779 None:intergenic 45.0%
GGTGACATTTAAGGATCCAG+AGG - chr8.3:67938205-67938224 MS.gene08528:intron 45.0%
GTTGGGATTGCAACTGAAGA+AGG + chr8.3:67939640-67939659 None:intergenic 45.0%
TACTACAATAGCAGCACCTG+TGG - chr8.3:67940067-67940086 MS.gene08528:CDS 45.0%
TATCCTTCTGTTGGAGCTTC+TGG - chr8.3:67939466-67939485 MS.gene08528:intron 45.0%
TTTGTTCCAGAGGCTTGTGA+AGG + chr8.3:67940110-67940129 None:intergenic 45.0%
! AAGGTGGTAGCTTTTAGCCT+AGG + chr8.3:67938518-67938537 None:intergenic 45.0%
! CAGTTTTCCATCAACCCACT+AGG - chr8.3:67938743-67938762 MS.gene08528:intron 45.0%
! GCACAAAGTATCCATGGTCA+TGG - chr8.3:67939535-67939554 MS.gene08528:intron 45.0%
!! AGTGGGTTGATGGAAAACTG+TGG + chr8.3:67938743-67938762 None:intergenic 45.0%
!! GGTTAGCTTGGGAAACTCAA+AGG - chr8.3:67937957-67937976 MS.gene08528:intron 45.0%
!! TTGGTGTCATTGGTGCCATT+AGG - chr8.3:67939044-67939063 MS.gene08528:intron 45.0%
!!! TATTTATTTACTATTTTTTT+TGG - chr8.3:67938575-67938594 MS.gene08528:CDS 5.0%
CACCAATGGCACCAAAGCTA+TGG - chr8.3:67939126-67939145 MS.gene08528:intron 50.0%
CAGCACCTGTGGTAGTAACA+AGG - chr8.3:67940078-67940097 MS.gene08528:CDS 50.0%
CATGCTCTTATAGCTGCCTC+TGG + chr8.3:67938224-67938243 None:intergenic 50.0%
CCTTGTGACCCAAAATGCTG+AGG + chr8.3:67939604-67939623 None:intergenic 50.0%
GATGCAAGCTATGGCACCAA+TGG - chr8.3:67939112-67939131 MS.gene08528:intron 50.0%
GATTGTACCACTCCATGTGG+AGG + chr8.3:67938697-67938716 None:intergenic 50.0%
GCAATCCCAACCATTAGTGC+TGG - chr8.3:67939649-67939668 MS.gene08528:intron 50.0%
GTGTGTGTGATGTGTGTGTG+TGG - chr8.3:67939085-67939104 MS.gene08528:CDS 50.0%
TCCTTCTGTTGGAGCTTCTG+GGG - chr8.3:67939468-67939487 MS.gene08528:intron 50.0%
TGTGTGATGTGTGTGTGTGG+TGG - chr8.3:67939088-67939107 MS.gene08528:CDS 50.0%
! TGTGGTGGAGATGCAAGCTA+TGG - chr8.3:67939103-67939122 MS.gene08528:intron 50.0%
!! CCTCAGCATTTTGGGTCACA+AGG - chr8.3:67939601-67939620 MS.gene08528:intron 50.0%
!! GCACTTTGGTGCCATAGCTT+TGG + chr8.3:67939140-67939159 None:intergenic 50.0%
!! TGCCATAGCTTTGGTGCCAT+TGG + chr8.3:67939131-67939150 None:intergenic 50.0%
!! TGTACTGCCTAGTGGGTTGA+TGG + chr8.3:67938753-67938772 None:intergenic 50.0%
CCTGTGGTAGTAACAAGGGC+CGG - chr8.3:67940083-67940102 MS.gene08528:CDS 55.0%
GGAAGGTTTAGGCCTCCACA+TGG - chr8.3:67938682-67938701 MS.gene08528:intron 55.0%
GTTTAGGCCTCCACATGGAG+TGG - chr8.3:67938687-67938706 MS.gene08528:intron 55.0%
TCCAGAGGCTTGTGAAGGAC+CGG + chr8.3:67940105-67940124 None:intergenic 55.0%
!! AGCTTCTGGGGCAATGGGTT+TGG - chr8.3:67939480-67939499 MS.gene08528:intron 55.0%
!! GTTGGAGCTTCTGGGGCAAT+GGG - chr8.3:67939475-67939494 MS.gene08528:intron 55.0%
!! TGTTGGAGCTTCTGGGGCAA+TGG - chr8.3:67939474-67939493 MS.gene08528:intron 55.0%
CCGGCCCTTGTTACTACCAC+AGG + chr8.3:67940086-67940105 None:intergenic 60.0%
GCCCCAGAAGCTCCAACAGA+AGG + chr8.3:67939472-67939491 None:intergenic 60.0%
GCCGGTCCTTCACAAGCCTC+TGG - chr8.3:67940101-67940120 MS.gene08528:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 67937869 67940157 67937869 ID=MS.gene08528
chr8.3 mRNA 67937869 67940157 67937869 ID=MS.gene08528.t1;Parent=MS.gene08528
chr8.3 exon 67939951 67940157 67939951 ID=MS.gene08528.t1.exon1;Parent=MS.gene08528.t1
chr8.3 CDS 67939951 67940157 67939951 ID=cds.MS.gene08528.t1;Parent=MS.gene08528.t1
chr8.3 exon 67939688 67939820 67939688 ID=MS.gene08528.t1.exon2;Parent=MS.gene08528.t1
chr8.3 CDS 67939688 67939820 67939688 ID=cds.MS.gene08528.t1;Parent=MS.gene08528.t1
chr8.3 exon 67939232 67939343 67939232 ID=MS.gene08528.t1.exon3;Parent=MS.gene08528.t1
chr8.3 CDS 67939232 67939343 67939232 ID=cds.MS.gene08528.t1;Parent=MS.gene08528.t1
chr8.3 exon 67939081 67939117 67939081 ID=MS.gene08528.t1.exon4;Parent=MS.gene08528.t1
chr8.3 CDS 67939081 67939117 67939081 ID=cds.MS.gene08528.t1;Parent=MS.gene08528.t1
chr8.3 exon 67938356 67938602 67938356 ID=MS.gene08528.t1.exon5;Parent=MS.gene08528.t1
chr8.3 CDS 67938356 67938602 67938356 ID=cds.MS.gene08528.t1;Parent=MS.gene08528.t1
chr8.3 exon 67937869 67937975 67937869 ID=MS.gene08528.t1.exon6;Parent=MS.gene08528.t1
chr8.3 CDS 67937869 67937975 67937869 ID=cds.MS.gene08528.t1;Parent=MS.gene08528.t1
Gene Sequence

>MS.gene08528

ATGTCTCAACAAAGACAACCTCATATGGTGAGTGGTAACAATCAAGACCAATATAATGACACAACATTTACCAAAATATTTGTTGGAGGGTTAGCTTGGGAAACTCAAAGGGAAACAATGAGAATTTATTTTGAACAATTTGGAGAGATCTTAGAAGCTGTAGTTATCACTGATAAGTATACTGGGAGATCTAAAGGTTATGGTTTTGTAAGTCCATTTTTCTTTTCCTTATATTTATTTTACTATGCTTCTCACATTTGTGCATAGTTTATATTTTACTTATATATTTATATGATCTTAGACAATGATTTTGCAATTTTTTTTTTTGTTTTTGTAGGTGACATTTAAGGATCCAGAGGCAGCTATAAGAGCATGTCAAAATCCATCTCCAGTTATTGATGGAAGAAGAGCAAATTGCAATATTGCTTGTCTTGGTGCTAACAAGAATCGATCACTAACTCTTCAGCATGGTTTTAATCCTTCTACCATTCTCTTTTATTTCTTTCTTTTTTTTTTTCTACTTGTTATACTATGATCAAATTTATAGTAAAATATCCTTTTTTTTTTAAAAAAAAAAAGGTATATTGTGTTATTTATTTGGTTTTTAATTTTCCCTGCCATTGTTCAACCTATCAATTTGATTCATCCTAGGCTAAAAGCTACCACCTTTTTTTTATTAAAGAAACTACCACTTTTTATGGTTGATTATTTATTTACTATTTTTTTTGGTACAAATTTACTAATTTATTTGTTGAAAAAAAATATGATTATATGTTTTATAAGATTTTAAAGGAAAAATTGAAATGGTGTGCAGGAAGGTTTAGGCCTCCACATGGAGTGGTACAATCAGTTCCTTATCATGGTTCTTCATCCACAGTTTTCCATCAACCCACTAGGCAGTACACATTTCCTTATTCAGCATATGGGTAACTTTTATGTTATGAATTTTTTTATGTTGAGCTAGTTATTTTTTATGAACGTTTGTATATTCTTCGTCTGTCCTTTTCTTTGATGTCAATTCTATGATATTTATCTGACAGATATTCTGGATATTCACAAGACACATTGTATCCTGCGGTAAGTGTTGATGTAATAAATTCCACATGTTTCAATTAATAGTGAAATCGGCAGAATCACGATGACACAATAATGGGAAATACTTCCATGACATAGTTTTGGTGTCATTGGTGCCATTAGGAAAATTCTGGCCATTGATGTGTGTGTGATGTGTGTGTGTGGTGGAGATGCAAGCTATGGCACCAATGGCACCAAAGCTATGGCACCAAAGTGCTACTCATAATTTTGTCAAAATCATGTTGGTTCATCGTAATTTTGTAAAATTGACTGTCAATCCAATATATATGTGATTAATTTGAAAGAATCATTATATCATCGTTATTTTGACAAAAGTTATATTTTAAAGAATCAACTAAATCACGGTTGCATCGTCATTTCATAGTGATTCAAACATACTTAGTAGAAGTAAACTTATAAGTTATATATTTTGATGCATTATTATTTATTGTTGTTTACACTTTTGTGTAAACTATTGCAGAACTATTACAATGTCTATGGAGTTCAACAATATTCACCTTACTATCCTTCTGTTGGAGCTTCTGGGGCAATGGGTTTGGTCCAAAATATGTACCCTTACTATGGTCAATATGCACAAAGTATCCATGGTCATGGTTTTGGAGTTCAATACCCCCAAATGACCCAAATTCCAATTTTGCCTCAGCATTTTGGGTCACAAGGAATATTGACTCTTCCTTCTTCAGTTGCAATCCCAACCATTAGTGCTGGTAATAATTCTAATACATTATGATATTATTTTTAAATTTTTATAATTTCTCTTCATTGTTGAGCAATTCTATTTCAAAATGGAGTTAGAATCACTAATTAATTTATTAGTAAAAAAACGAAAGAATAGTTCATTATGTATTCGTTGTAAGGCTGCTTATTTAAAATATATATATATATAGACACACACACTTAGAAACTAAATCGACCCCCCTGAAAAGACTAATATGATCAAGAATTTTCAATTTTAAGAATTGTTTTGAAACTTCATTAATATCAAATATCAATTTATCTTACCTTTATAATTTGAGAGATTAAACTAGTGATTTAATTATTTTAAATTTTGTATTGAAGACTAAATTGTAACAATTTTCAGCAACAGTAACTGGAACCACAACTACTACAATAGCAGCACCTGTGGTAGTAACAAGGGCCGGTCCTTCACAAGCCTCTGGAACAAATACTGAACAACAACATTCTACATCTTGA
Protein sequence

>MS.gene08528.t1

MSQQRQPHMVSGNNQDQYNDTTFTKIFVGGLAWETQRETMRIYFEQFGEILEAVVITDKYTGRSKGYGFVTFKDPEAAIRACQNPSPVIDGRRANCNIACLGANKNRSLTLQHGRFRPPHGVVQSVPYHGSSSTVFHQPTRQYTFPYSAYGYSGYSQDTLYPANYYNVYGVQQYSPYYPSVGASGAMGLVQNMYPYYGQYAQSIHGHGFGVQYPQMTQIPILPQHFGSQGILTLPSSVAIPTISAVTGTTTTTIAAPVVVTRAGPSQASGTNTEQQHSTS