AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene060491

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060491.t1 MTR_8g024520 94.662 281 13 2 1 280 1 280 0.0 511
MS.gene060491.t1 MTR_8g024520 93.262 282 17 2 1 280 1 282 0.0 510
MS.gene060491.t1 MTR_3g116130 56.000 200 72 9 18 209 23 214 2.34e-56 182
MS.gene060491.t1 MTR_3g116130 56.000 200 72 9 18 209 23 214 1.46e-55 183
MS.gene060491.t1 MTR_4g035310 45.865 266 109 10 9 244 1 261 1.10e-50 167
MS.gene060491.t1 MTR_4g035310 55.814 172 63 3 9 170 1 169 1.29e-50 167
MS.gene060491.t1 MTR_3g092490 52.000 175 75 6 1 170 1 171 5.54e-48 160
MS.gene060491.t1 MTR_3g092490 54.194 155 65 4 1 153 1 151 7.25e-48 157
MS.gene060491.t1 MTR_3g092490 52.273 176 74 6 1 170 1 172 1.96e-47 159
MS.gene060491.t1 MTR_5g064120 77.011 87 20 0 15 101 7 93 2.16e-45 154
MS.gene060491.t1 MTR_7g117495 63.636 99 34 1 9 105 1 99 1.25e-41 144
MS.gene060491.t1 MTR_7g117495 63.636 99 34 1 9 105 1 99 1.73e-41 144
MS.gene060491.t1 MTR_4g088010 48.810 84 43 0 20 103 72 155 5.96e-19 85.9
MS.gene060491.t1 MTR_8g463890 42.857 98 56 0 5 102 54 151 7.10e-19 86.3
MS.gene060491.t1 MTR_2g026180 36.066 122 74 2 25 143 5 125 9.13e-19 86.3
MS.gene060491.t1 MTR_3g055990 42.466 73 41 1 20 92 2 73 6.67e-17 80.5
MS.gene060491.t1 MTR_3g055990 42.466 73 41 1 20 92 2 73 6.75e-17 80.5
MS.gene060491.t1 MTR_7g088140 43.373 83 47 0 20 102 123 205 2.86e-16 79.0
MS.gene060491.t1 MTR_7g088140 43.373 83 47 0 20 102 123 205 4.58e-16 78.2
MS.gene060491.t1 MTR_5g097580 36.066 122 72 3 25 143 7 125 5.69e-16 77.8
MS.gene060491.t1 MTR_5g097580 36.066 122 72 3 25 143 7 125 6.22e-16 77.8
MS.gene060491.t1 MTR_5g088220 42.169 83 48 0 20 102 161 243 1.20e-15 77.0
MS.gene060491.t1 MTR_4g070190 43.421 76 42 1 25 99 9 84 1.23e-14 70.5
MS.gene060491.t1 MTR_4g070080 43.590 78 39 2 25 99 7 82 2.24e-14 68.9
MS.gene060491.t1 MTR_1g073690 36.250 80 50 1 20 99 2 80 3.67e-14 72.4
MS.gene060491.t1 MTR_4g070080 43.590 78 39 2 25 99 7 82 6.36e-14 68.6
MS.gene060491.t1 MTR_3g084040 40.580 69 41 0 24 92 36 104 9.94e-14 67.4
MS.gene060491.t1 MTR_4g109540 41.026 78 45 1 24 100 42 119 1.44e-13 69.3
MS.gene060491.t1 MTR_4g070140 43.421 76 42 1 25 99 9 84 1.67e-13 67.8
MS.gene060491.t1 MTR_3g084040 40.909 66 39 0 24 89 36 101 2.63e-13 65.1
MS.gene060491.t1 MTR_7g013730 38.961 77 46 1 25 100 41 117 2.91e-13 68.6
MS.gene060491.t1 MTR_4g063560 45.070 71 38 1 24 93 33 103 3.09e-13 65.1
MS.gene060491.t1 MTR_3g025880 39.024 82 41 2 24 100 44 121 7.81e-13 68.6
MS.gene060491.t1 MTR_7g105260 33.333 99 62 2 25 122 33 128 7.75e-12 62.0
MS.gene060491.t1 MTR_7g013740 41.026 78 41 2 25 99 41 116 1.75e-11 61.2
MS.gene060491.t1 MTR_3g082650 40.909 66 39 0 25 90 19 84 1.85e-11 63.9
MS.gene060491.t1 MTR_3g080130 34.783 69 41 1 24 92 36 100 2.69e-11 60.5
MS.gene060491.t1 MTR_4g109540 37.209 86 45 2 24 100 42 127 3.33e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060491.t1 AT2G46780 56.031 257 74 11 1 223 1 252 1.02e-75 234
MS.gene060491.t1 AT2G46780 56.031 257 74 11 1 223 1 252 1.02e-75 234
MS.gene060491.t1 AT1G33470 55.414 157 63 4 20 170 3 158 7.75e-53 172
MS.gene060491.t1 AT1G33470 55.063 158 63 5 20 170 3 159 4.01e-51 168
MS.gene060491.t1 AT1G33470 55.063 158 63 5 20 170 3 159 4.70e-51 168
MS.gene060491.t1 AT1G76460 41.887 265 115 10 18 266 18 259 1.74e-49 165
MS.gene060491.t1 AT1G20880 50.242 207 79 8 18 209 18 215 2.19e-49 164
MS.gene060491.t1 AT1G20880 50.242 207 79 8 18 209 18 215 2.19e-49 164
MS.gene060491.t1 AT1G20880 50.242 207 79 8 18 209 18 215 2.19e-49 164
MS.gene060491.t1 AT1G22910 51.899 158 70 3 20 171 9 166 6.19e-45 152
MS.gene060491.t1 AT1G22910 51.899 158 70 3 20 171 9 166 1.41e-44 151
MS.gene060491.t1 AT1G22910 51.899 158 70 3 20 171 9 166 6.59e-44 152
MS.gene060491.t1 AT1G22330 71.765 85 24 0 18 102 11 95 3.15e-40 141
MS.gene060491.t1 AT1G78260 71.429 84 24 0 18 101 11 94 7.44e-40 140
MS.gene060491.t1 AT1G78260 71.429 84 24 0 18 101 11 94 1.89e-39 139
MS.gene060491.t1 AT3G54770 50.000 130 64 1 9 137 1 130 1.04e-38 136
MS.gene060491.t1 AT3G54770 50.000 130 64 1 9 137 1 130 1.06e-38 137
MS.gene060491.t1 AT5G11412 63.855 83 30 0 20 102 48 130 5.85e-35 124
MS.gene060491.t1 AT3G06970 49.138 116 52 2 20 128 8 123 1.87e-31 119
MS.gene060491.t1 AT5G53680 57.143 84 36 0 20 103 9 92 6.40e-31 114
MS.gene060491.t1 AT5G53720 57.500 80 34 0 24 103 9 88 1.44e-26 100
MS.gene060491.t1 AT3G54770 51.020 98 37 1 40 137 1 87 5.96e-26 102
MS.gene060491.t1 AT2G22090 50.602 83 41 0 20 102 100 182 8.39e-20 88.2
MS.gene060491.t1 AT2G22090 50.602 83 41 0 20 102 100 182 8.74e-20 88.2
MS.gene060491.t1 AT3G15010 40.816 98 58 0 5 102 56 153 2.10e-17 81.6
MS.gene060491.t1 AT3G15010 40.816 98 58 0 5 102 56 153 2.10e-17 81.6
MS.gene060491.t1 AT5G47620 48.529 68 34 1 25 92 7 73 2.14e-17 82.0
MS.gene060491.t1 AT5G47620 48.529 68 34 1 25 92 7 73 2.14e-17 82.0
MS.gene060491.t1 AT5G47620 48.529 68 34 1 25 92 7 73 2.14e-17 82.0
MS.gene060491.t1 AT5G47620 47.826 69 35 1 24 92 6 73 2.47e-17 82.0
MS.gene060491.t1 AT4G26650 40.278 72 42 1 21 92 12 82 1.95e-16 79.3
MS.gene060491.t1 AT5G55550 39.726 73 43 1 20 92 2 73 1.96e-16 79.3
MS.gene060491.t1 AT5G55550 39.726 73 43 1 20 92 2 73 1.96e-16 79.3
MS.gene060491.t1 AT5G55550 39.726 73 43 1 20 92 2 73 1.96e-16 79.3
MS.gene060491.t1 AT5G55550 39.726 73 43 1 20 92 2 73 1.96e-16 79.3
MS.gene060491.t1 AT5G55550 39.726 73 43 1 20 92 2 73 1.96e-16 79.3
MS.gene060491.t1 AT4G26650 40.278 72 42 1 21 92 9 79 2.02e-16 79.3
MS.gene060491.t1 AT5G55550 39.726 73 43 1 20 92 2 73 2.04e-16 79.3
MS.gene060491.t1 AT5G55550 39.726 73 43 1 20 92 2 73 2.04e-16 79.3
MS.gene060491.t1 AT5G55550 39.726 73 43 1 20 92 2 73 2.04e-16 79.3
MS.gene060491.t1 AT5G55550 39.726 73 43 1 20 92 2 73 2.04e-16 79.3
MS.gene060491.t1 AT5G55550 39.726 73 43 1 20 92 2 73 2.41e-16 79.0
MS.gene060491.t1 AT5G55550 39.726 73 43 1 20 92 2 73 2.50e-16 79.0
MS.gene060491.t1 AT3G07810 46.479 71 31 2 25 92 7 73 8.76e-16 77.4
MS.gene060491.t1 AT3G07810 46.479 71 31 2 25 92 7 73 9.11e-16 77.4
MS.gene060491.t1 AT4G39260 43.421 76 42 1 25 99 7 82 1.66e-15 72.0
MS.gene060491.t1 AT2G41060 40.964 83 49 0 20 102 124 206 1.66e-15 76.6
MS.gene060491.t1 AT2G41060 40.964 83 49 0 20 102 124 206 1.66e-15 76.6
MS.gene060491.t1 AT2G41060 40.964 83 49 0 20 102 124 206 1.66e-15 76.6
MS.gene060491.t1 AT3G23830 41.026 78 45 1 24 100 35 112 1.87e-15 72.0
MS.gene060491.t1 AT3G23830 41.026 78 45 1 24 100 35 112 1.87e-15 72.0
MS.gene060491.t1 AT4G13850 44.444 72 36 1 24 95 35 102 1.90e-15 71.6
MS.gene060491.t1 AT3G56860 42.169 83 48 0 20 102 136 218 2.12e-15 76.3
MS.gene060491.t1 AT3G56860 42.169 83 48 0 20 102 136 218 2.12e-15 76.3
MS.gene060491.t1 AT3G56860 42.169 83 48 0 20 102 136 218 2.12e-15 76.3
MS.gene060491.t1 AT3G56860 42.169 83 48 0 20 102 136 218 2.12e-15 76.3
MS.gene060491.t1 AT3G56860 42.169 83 48 0 20 102 136 218 2.12e-15 76.3
MS.gene060491.t1 AT3G56860 42.169 83 48 0 20 102 136 218 2.12e-15 76.3
MS.gene060491.t1 AT3G56860 42.169 83 48 0 20 102 136 218 2.12e-15 76.3
MS.gene060491.t1 AT3G56860 42.169 83 48 0 20 102 136 218 2.12e-15 76.3
MS.gene060491.t1 AT3G56860 42.169 83 48 0 20 102 136 218 2.12e-15 76.3
MS.gene060491.t1 AT3G56860 42.169 83 48 0 20 102 136 218 2.12e-15 76.3
MS.gene060491.t1 AT3G56860 42.169 83 48 0 20 102 136 218 2.12e-15 76.3
MS.gene060491.t1 AT4G39260 43.421 76 42 1 25 99 7 82 2.47e-15 70.5
MS.gene060491.t1 AT4G13850 44.444 72 36 1 24 95 35 102 2.76e-15 71.6
MS.gene060491.t1 AT4G13850 44.444 72 36 1 24 95 35 102 3.39e-15 71.6
MS.gene060491.t1 AT4G14300 41.250 80 46 1 20 99 2 80 3.40e-15 75.5
MS.gene060491.t1 AT4G14300 41.250 80 46 1 20 99 2 80 3.40e-15 75.5
MS.gene060491.t1 AT4G13850 44.444 72 36 1 24 95 35 102 3.79e-15 71.6
MS.gene060491.t1 AT2G19380 44.444 81 45 0 20 100 404 484 4.02e-15 75.5
MS.gene060491.t1 AT4G39260 43.421 76 42 1 25 99 7 82 4.42e-15 72.0
MS.gene060491.t1 AT1G17640 44.444 72 39 1 25 96 67 137 6.25e-15 74.3
MS.gene060491.t1 AT1G17640 44.444 72 39 1 25 96 67 137 6.25e-15 74.3
MS.gene060491.t1 AT1G17640 44.444 72 39 1 25 96 67 137 6.25e-15 74.3
MS.gene060491.t1 AT2G22100 44.156 77 43 0 20 96 159 235 7.52e-15 74.3
MS.gene060491.t1 AT4G39260 50.943 53 26 0 25 77 7 59 1.15e-14 68.9
MS.gene060491.t1 AT2G33410 41.333 75 43 1 25 99 7 80 2.06e-14 73.2
MS.gene060491.t1 AT2G21660 42.105 76 43 1 25 99 9 84 2.63e-14 69.3
MS.gene060491.t1 AT2G21660 42.105 76 43 1 25 99 9 84 3.72e-14 69.3
MS.gene060491.t1 AT5G61030 43.478 69 35 1 24 92 40 104 1.55e-13 69.7
MS.gene060491.t1 AT5G61030 43.478 69 35 1 24 92 40 104 1.55e-13 69.7
MS.gene060491.t1 AT1G74230 41.558 77 45 0 24 100 34 110 1.85e-13 69.3
MS.gene060491.t1 AT1G18630 39.785 93 49 3 5 92 15 105 3.36e-13 66.2
MS.gene060491.t1 AT3G26420 45.614 57 31 0 25 81 8 64 5.53e-13 67.4
MS.gene060491.t1 AT5G59865 51.020 49 24 0 24 72 26 74 1.11e-11 60.8
MS.gene060491.t1 AT1G58470 39.189 74 41 1 25 94 7 80 1.27e-11 64.7
MS.gene060491.t1 AT1G58470 39.189 74 41 1 25 94 7 80 1.27e-11 64.7
MS.gene060491.t1 AT1G22910 37.037 108 62 3 70 171 8 115 3.38e-11 61.6
MS.gene060491.t1 AT3G13224 32.584 89 58 2 25 112 20 107 5.51e-11 62.8

Find 78 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCCTTCTGTTGGAGCTTC+TGG 0.055135 8.1:-75832344 MS.gene060491:CDS
CATAGTAAGGGTACATATTT+TGG 0.172442 8.1:+75832306 None:intergenic
TAACCCTCCAACAAATATTT+TGG 0.186878 8.1:+75833899 None:intergenic
GCTGAGGCAAAACTGGAATT+TGG 0.187376 8.1:+75832225 None:intergenic
AACTGGAATTTGGGTCATTT+GGG 0.205081 8.1:+75832235 None:intergenic
TATTTATCTGACAGATATTC+TGG 0.249082 8.1:-75832914 MS.gene060491:intron
AGTATCCATGGTCATGGTTT+TGG 0.271120 8.1:-75832269 MS.gene060491:CDS
AGCTTCTGGGGCAATGGGTT+TGG 0.272297 8.1:-75832330 MS.gene060491:CDS
CCAAAATGCTGAGGCAAAAC+TGG 0.286123 8.1:+75832218 None:intergenic
AAACTGGAATTTGGGTCATT+TGG 0.293455 8.1:+75832234 None:intergenic
CATGCTCTTATAGCTGCTTC+TGG 0.298266 8.1:+75833592 None:intergenic
ACATTTCCTTATTCAGCATA+TGG 0.317744 8.1:-75833035 MS.gene060491:CDS
ATCCTTCTGTTGGAGCTTCT+GGG 0.321902 8.1:-75832343 MS.gene060491:CDS
AGTTACCCATATGCTGAATA+AGG 0.334120 8.1:+75833029 None:intergenic
GTTATCACTGATAAGTATAC+TGG 0.334320 8.1:-75833807 MS.gene060491:CDS
TGTTGGAGCTTCTGGGGCAA+TGG 0.334769 8.1:-75832336 MS.gene060491:CDS
CAGAACTATTACAATGTCTA+TGG 0.345598 8.1:-75832389 MS.gene060491:CDS
GCTCTTCGTCCATCAATAAC+TGG 0.354325 8.1:+75833556 None:intergenic
AGAATTATTACCAGCACTAA+TGG 0.360267 8.1:+75832151 None:intergenic
CAAAATATGTACCCTTACTA+TGG 0.370998 8.1:-75832305 MS.gene060491:CDS
GTTGGAGCTTCTGGGGCAAT+GGG 0.372650 8.1:-75832335 MS.gene060491:CDS
AATGGTGTGCAGGAAGGTTT+AGG 0.381511 8.1:-75833136 MS.gene060491:intron
ACTGGGAGATCTAAAGGTTA+TGG 0.382688 8.1:-75833789 MS.gene060491:CDS
CTGAGGCAAAACTGGAATTT+GGG 0.394905 8.1:+75832226 None:intergenic
TAAATGTTGTGTCATTATAT+TGG 0.402817 8.1:+75833922 None:intergenic
ACATTTACCAAAATATTTGT+TGG 0.412578 8.1:-75833906 MS.gene060491:CDS
AATCCATCTCCAGTTATTGA+TGG 0.418329 8.1:-75833565 MS.gene060491:CDS
TATTTGTTGGAGGGTTAGCT+TGG 0.423354 8.1:-75833893 MS.gene060491:CDS
TATTACCAGCACTAATGGTT+GGG 0.433671 8.1:+75832156 None:intergenic
TGTGCATATTGACCATAGTA+AGG 0.433964 8.1:+75832293 None:intergenic
CATTTCCTTATTCAGCATAT+GGG 0.440818 8.1:-75833034 MS.gene060491:intron
TGTACTGCCTAGTGGGTTGA+TGG 0.442879 8.1:+75833057 None:intergenic
AATTGCAATATTGCTTCTCT+TGG 0.445540 8.1:-75833532 MS.gene060491:CDS
TTGTTACCACTCACCATATG+AGG 0.446616 8.1:+75833951 None:intergenic
ATTTGTTGGAGGGTTAGCTT+GGG 0.450733 8.1:-75833892 MS.gene060491:CDS
GTACAATCAGTTCCTTATCA+TGG 0.451060 8.1:-75833098 MS.gene060491:CDS
TCAACAAAGACAACCTCATA+TGG 0.453555 8.1:-75833964 MS.gene060491:CDS
TCACCTTACTATCCTTCTGT+TGG 0.471067 8.1:-75832353 MS.gene060491:CDS
GCTCCAACAGAAGGATAGTA+AGG 0.482209 8.1:+75832350 None:intergenic
GCTGGTCCTTCACAAGCCTC+TGG 0.489668 8.1:-75831655 MS.gene060491:CDS
AAGTATACTGGGAGATCTAA+AGG 0.490906 8.1:-75833795 MS.gene060491:CDS
TTATTACCAGCACTAATGGT+TGG 0.519138 8.1:+75832155 None:intergenic
CCAGCCCTTGTTACTGCCAC+AGG 0.522193 8.1:+75831673 None:intergenic
TTTACCAAAATATTTGTTGG+AGG 0.522261 8.1:-75833903 MS.gene060491:CDS
TCCTTCTGTTGGAGCTTCTG+GGG 0.522825 8.1:-75832342 MS.gene060491:CDS
CAGCACCTGTGGCAGTAACA+AGG 0.532377 8.1:-75831678 MS.gene060491:CDS
CGTCCATCAATAACTGGAGA+TGG 0.535151 8.1:+75833562 None:intergenic
GGTTAGCTTGGGAAACTCAA+AGG 0.538530 8.1:-75833881 MS.gene060491:CDS
AGGAAATGTGTACTGCCTAG+TGG 0.539088 8.1:+75833049 None:intergenic
TGCTGCTATTGTAGTAGTTG+TGG 0.540042 8.1:+75831696 None:intergenic
GTGGATGAAGAACCATGATA+AGG 0.546300 8.1:+75833086 None:intergenic
TTGTTCAGTATCTGTTCCAG+AGG 0.547325 8.1:+75831639 None:intergenic
TTACCAAAATATTTGTTGGA+GGG 0.548943 8.1:-75833902 MS.gene060491:CDS
GTTGGGATTGCAACTGAAGA+AGG 0.551412 8.1:+75832173 None:intergenic
CAATATGCACAAAGTATCCA+TGG 0.551625 8.1:-75832281 MS.gene060491:CDS
CCTGTGGCAGTAACAAGGGC+TGG 0.554680 8.1:-75831673 MS.gene060491:CDS
GCAATCCCAACCATTAGTGC+TGG 0.558559 8.1:-75832161 MS.gene060491:intron
CTGGAATTTGGGTCATTTGG+GGG 0.562855 8.1:+75832237 None:intergenic
GCCCCAGAAGCTCCAACAGA+AGG 0.575329 8.1:+75832341 None:intergenic
GCACAAAGTATCCATGGTCA+TGG 0.581950 8.1:-75832275 MS.gene060491:CDS
ACTGGAATTTGGGTCATTTG+GGG 0.588666 8.1:+75832236 None:intergenic
GTTAGCTTGGGAAACTCAAA+GGG 0.597037 8.1:-75833880 MS.gene060491:CDS
GTGCATATTGACCATAGTAA+GGG 0.598572 8.1:+75832294 None:intergenic
AGCACCTGTGGCAGTAACAA+GGG 0.600833 8.1:-75831677 MS.gene060491:CDS
TACTACAATAGCAGCACCTG+TGG 0.605257 8.1:-75831689 MS.gene060491:CDS
TTATCACTGATAAGTATACT+GGG 0.624818 8.1:-75833806 MS.gene060491:CDS
TCTGTTCCAGAGGCTTGTGA+AGG 0.628522 8.1:+75831649 None:intergenic
CGATCACTAACTCTTCAGCA+TGG 0.635608 8.1:-75833496 MS.gene060491:intron
GAACTCCAAAACCATGACCA+TGG 0.639031 8.1:+75832264 None:intergenic
GGAAGGTTTAGGCCTCCACA+TGG 0.645450 8.1:-75833125 MS.gene060491:CDS
GGAAATGTGTACTGCCTAGT+GGG 0.657310 8.1:+75833050 None:intergenic
AGACAACCTCATATGGTGAG+TGG 0.661054 8.1:-75833957 MS.gene060491:CDS
CCTTGTGACCCAAAATGCTG+AGG 0.666097 8.1:+75832209 None:intergenic
GTTTAGGCCTCCACATGGAG+TGG 0.666440 8.1:-75833120 MS.gene060491:CDS
GATTGTACCACTCCATGTGG+AGG 0.680340 8.1:+75833113 None:intergenic
ACTGATTGTACCACTCCATG+TGG 0.705430 8.1:+75833110 None:intergenic
ACAAGACACATTGTATCCTG+CGG 0.715658 8.1:-75832885 MS.gene060491:intron
AGTGGGTTGATGGAAAACTG+TGG 0.742505 8.1:+75833067 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTATAAAAAAAAAA+GGG - chr8.1:75832204-75832223 MS.gene060491:CDS 0.0%
!! AAATATTATGAAAAGAAAAA+TGG + chr8.1:75831837-75831856 None:intergenic 10.0%
!! TAGTTTCTTTAATAAAAAAA+AGG + chr8.1:75832315-75832334 None:intergenic 10.0%
!!! TATGTTTTATAAGATTTTAA+AGG - chr8.1:75832422-75832441 MS.gene060491:intron 10.0%
!! AATTAATTTAGTATCAAACT+AGG - chr8.1:75833546-75833565 MS.gene060491:CDS 15.0%
!! CAAATTAATCACATATATAT+TGG + chr8.1:75833004-75833023 None:intergenic 15.0%
!! TTTCTTTAATAAAAAAAAGG+TGG + chr8.1:75832312-75832331 None:intergenic 15.0%
!!! TTTTAAAGGAAAAATTGAAA+TGG - chr8.1:75832436-75832455 MS.gene060491:intron 15.0%
!! AAATAATCAACCATAAAAAG+TGG + chr8.1:75832338-75832357 None:intergenic 20.0%
!! AAGAAAGAAATAAAAGAGAA+TGG + chr8.1:75832134-75832153 None:intergenic 20.0%
!! ACATTTACCAAAATATTTGT+TGG - chr8.1:75831684-75831703 MS.gene060491:CDS 20.0%
!! GTTTCAATTAATAGTGAAAT+CGG - chr8.1:75832755-75832774 MS.gene060491:intron 20.0%
!! TAAATATATATAGCCACAAA+TGG + chr8.1:75831911-75831930 None:intergenic 20.0%
!! TTAAAGAATCAACTAAATCA+CGG - chr8.1:75833066-75833085 MS.gene060491:CDS 20.0%
!! TTACTTATATATTCCATTTG+TGG - chr8.1:75831895-75831914 MS.gene060491:intron 20.0%
!! TTTAATCTCTCAAATTATAG+AGG + chr8.1:75833802-75833821 None:intergenic 20.0%
!!! AGAATGTATTTTGAACAATT+TGG - chr8.1:75831741-75831760 MS.gene060491:intron 20.0%
!!! ATTTTTATAGGTGACATTTA+AGG - chr8.1:75831973-75831992 MS.gene060491:intron 20.0%
!!! GGTATATTGTGTTATTTATT+TGG - chr8.1:75832225-75832244 MS.gene060491:CDS 20.0%
!!! TAAATGTTGTGTCATTATAT+TGG + chr8.1:75831671-75831690 None:intergenic 20.0%
!!! TAATTTTGTCAAAATCATGT+TGG - chr8.1:75832946-75832965 MS.gene060491:intron 20.0%
! AAATAAAAGAGAATGGTAGA+AGG + chr8.1:75832127-75832146 None:intergenic 25.0%
! CATAATTGACATCAAAGAAA+AGG + chr8.1:75832655-75832674 None:intergenic 25.0%
! TATTTATCTGACAGATATTC+TGG - chr8.1:75832676-75832695 MS.gene060491:intron 25.0%
! TCACTATTAATTGAAACATG+TGG + chr8.1:75832752-75832771 None:intergenic 25.0%
! TGAGCAATTCTATTTCAAAA+TGG - chr8.1:75833510-75833529 MS.gene060491:CDS 25.0%
! TTATCACTGATAAGTATACT+GGG - chr8.1:75831784-75831803 MS.gene060491:intron 25.0%
! TTCATTATGTATTCGTTGTA+AGG - chr8.1:75833629-75833648 MS.gene060491:intron 25.0%
!! TTACCAAAATATTTGTTGGA+GGG - chr8.1:75831688-75831707 MS.gene060491:CDS 25.0%
!! TTTACCAAAATATTTGTTGG+AGG - chr8.1:75831687-75831706 MS.gene060491:CDS 25.0%
!!! AAAGAAACTACCACTTTTTA+TGG - chr8.1:75832325-75832344 MS.gene060491:CDS 25.0%
!!! TTGATCATATTAGTCTTTTC+AGG + chr8.1:75833718-75833737 None:intergenic 25.0%
AATCACGATGACACAATAAT+GGG - chr8.1:75832781-75832800 MS.gene060491:intron 30.0%
AATTGCAATATTGCTTCTCT+TGG - chr8.1:75832058-75832077 MS.gene060491:intron 30.0%
ACATTTCCTTATTCAGCATA+TGG - chr8.1:75832555-75832574 MS.gene060491:intron 30.0%
AGAATTATTACCAGCACTAA+TGG + chr8.1:75833442-75833461 None:intergenic 30.0%
CAAAATATGTACCCTTACTA+TGG - chr8.1:75833285-75833304 MS.gene060491:intron 30.0%
CAAAATTATGAGTAGCACTT+TGG + chr8.1:75832934-75832953 None:intergenic 30.0%
CAGAACTATTACAATGTCTA+TGG - chr8.1:75833201-75833220 MS.gene060491:intron 30.0%
CATTTCCTTATTCAGCATAT+GGG - chr8.1:75832556-75832575 MS.gene060491:intron 30.0%
CTAGGATGAATCAAATTGAT+AGG + chr8.1:75832278-75832297 None:intergenic 30.0%
CTATCAATTTGATTCATCCT+AGG - chr8.1:75832276-75832295 MS.gene060491:CDS 30.0%
GTTATCACTGATAAGTATAC+TGG - chr8.1:75831783-75831802 MS.gene060491:intron 30.0%
! ATACTTCCATGACATAGTTT+TGG - chr8.1:75832806-75832825 MS.gene060491:intron 30.0%
! CAAATTGATAGGTTGAACAA+TGG + chr8.1:75832267-75832286 None:intergenic 30.0%
! TAACCCTCCAACAAATATTT+TGG + chr8.1:75831694-75831713 None:intergenic 30.0%
!! CATAGTAAGGGTACATATTT+TGG + chr8.1:75833287-75833306 None:intergenic 30.0%
!!! ATCATATTAGTCTTTTCAGG+TGG + chr8.1:75833715-75833734 None:intergenic 30.0%
!!! TCATATTAGTCTTTTCAGGT+GGG + chr8.1:75833714-75833733 None:intergenic 30.0%
AAACTGGAATTTGGGTCATT+TGG + chr8.1:75833359-75833378 None:intergenic 35.0%
AACTGGAATTTGGGTCATTT+GGG + chr8.1:75833358-75833377 None:intergenic 35.0%
AAGTATACTGGGAGATCTAA+AGG - chr8.1:75831795-75831814 MS.gene060491:intron 35.0%
AATCCATCTCCAGTTATTGA+TGG - chr8.1:75832025-75832044 MS.gene060491:intron 35.0%
AGTTACCCATATGCTGAATA+AGG + chr8.1:75832564-75832583 None:intergenic 35.0%
GAAAAATTGAAATGGTGTGC+AGG - chr8.1:75832444-75832463 MS.gene060491:intron 35.0%
GAATCACGATGACACAATAA+TGG - chr8.1:75832780-75832799 MS.gene060491:intron 35.0%
GTACAATCAGTTCCTTATCA+TGG - chr8.1:75832492-75832511 MS.gene060491:intron 35.0%
GTGCATATTGACCATAGTAA+GGG + chr8.1:75833299-75833318 None:intergenic 35.0%
TATTACCAGCACTAATGGTT+GGG + chr8.1:75833437-75833456 None:intergenic 35.0%
TCAACAAAGACAACCTCATA+TGG - chr8.1:75831626-75831645 MS.gene060491:CDS 35.0%
TGTGCATATTGACCATAGTA+AGG + chr8.1:75833300-75833319 None:intergenic 35.0%
TTATTACCAGCACTAATGGT+TGG + chr8.1:75833438-75833457 None:intergenic 35.0%
! CAATATGCACAAAGTATCCA+TGG - chr8.1:75833309-75833328 MS.gene060491:intron 35.0%
AATGACCAGGATTTCTCCAA+TGG + chr8.1:75832843-75832862 None:intergenic 40.0%
AATTGAAATGGTGTGCAGGA+AGG - chr8.1:75832448-75832467 MS.gene060491:intron 40.0%
ACAAGACACATTGTATCCTG+CGG - chr8.1:75832705-75832724 MS.gene060491:intron 40.0%
ACTGGAATTTGGGTCATTTG+GGG + chr8.1:75833357-75833376 None:intergenic 40.0%
ACTGGGAGATCTAAAGGTTA+TGG - chr8.1:75831801-75831820 MS.gene060491:intron 40.0%
ATGGCACCAAAACTATGTCA+TGG + chr8.1:75832815-75832834 None:intergenic 40.0%
ATTACATCAACACTTACCGC+AGG + chr8.1:75832724-75832743 None:intergenic 40.0%
CTGAGGCAAAACTGGAATTT+GGG + chr8.1:75833367-75833386 None:intergenic 40.0%
GTGGATGAAGAACCATGATA+AGG + chr8.1:75832507-75832526 None:intergenic 40.0%
TCACCTTACTATCCTTCTGT+TGG - chr8.1:75833237-75833256 MS.gene060491:intron 40.0%
TGCTGCTATTGTAGTAGTTG+TGG + chr8.1:75833897-75833916 None:intergenic 40.0%
TTGTTACCACTCACCATATG+AGG + chr8.1:75831642-75831661 None:intergenic 40.0%
TTGTTCAGTATCTGTTCCAG+AGG + chr8.1:75833954-75833973 None:intergenic 40.0%
! AGTATCCATGGTCATGGTTT+TGG - chr8.1:75833321-75833340 MS.gene060491:intron 40.0%
! CAGTTTTGCCTCAGCATTTT+GGG - chr8.1:75833373-75833392 MS.gene060491:intron 40.0%
! GTTAGCTTGGGAAACTCAAA+GGG - chr8.1:75831710-75831729 MS.gene060491:intron 40.0%
! TGATAGGTTGAACAATGGCA+GGG + chr8.1:75832262-75832281 None:intergenic 40.0%
! TTGATAGGTTGAACAATGGC+AGG + chr8.1:75832263-75832282 None:intergenic 40.0%
!! ATTTGTTGGAGGGTTAGCTT+GGG - chr8.1:75831698-75831717 MS.gene060491:CDS 40.0%
!! TATTTGTTGGAGGGTTAGCT+TGG - chr8.1:75831697-75831716 MS.gene060491:CDS 40.0%
!!! TGACATAGTTTTGGTGCCAT+TGG - chr8.1:75832815-75832834 MS.gene060491:intron 40.0%
AATGGTGTGCAGGAAGGTTT+AGG - chr8.1:75832454-75832473 MS.gene060491:intron 45.0%
ACTGATTGTACCACTCCATG+TGG + chr8.1:75832483-75832502 None:intergenic 45.0%
AGACAACCTCATATGGTGAG+TGG - chr8.1:75831633-75831652 MS.gene060491:CDS 45.0%
AGGAAATGTGTACTGCCTAG+TGG + chr8.1:75832544-75832563 None:intergenic 45.0%
ATCCTGGTCATTGATGTGTG+TGG - chr8.1:75832851-75832870 MS.gene060491:intron 45.0%
ATCCTTCTGTTGGAGCTTCT+GGG - chr8.1:75833247-75833266 MS.gene060491:intron 45.0%
CATGCTCTTATAGCTGCTTC+TGG + chr8.1:75832001-75832020 None:intergenic 45.0%
CCAAAATGCTGAGGCAAAAC+TGG + chr8.1:75833375-75833394 None:intergenic 45.0%
CGATCACTAACTCTTCAGCA+TGG - chr8.1:75832094-75832113 MS.gene060491:intron 45.0%
CGTCCATCAATAACTGGAGA+TGG + chr8.1:75832031-75832050 None:intergenic 45.0%
CTGGAATTTGGGTCATTTGG+GGG + chr8.1:75833356-75833375 None:intergenic 45.0%
GAACTCCAAAACCATGACCA+TGG + chr8.1:75833329-75833348 None:intergenic 45.0%
GATGCAAGTTATGGCACCAA+TGG - chr8.1:75832892-75832911 MS.gene060491:CDS 45.0%
GATTTCTCCAATGGCACCAA+TGG + chr8.1:75832834-75832853 None:intergenic 45.0%
GCTCCAACAGAAGGATAGTA+AGG + chr8.1:75833243-75833262 None:intergenic 45.0%
GCTCTTCGTCCATCAATAAC+TGG + chr8.1:75832037-75832056 None:intergenic 45.0%
GCTGAGGCAAAACTGGAATT+TGG + chr8.1:75833368-75833387 None:intergenic 45.0%
GGAAATGTGTACTGCCTAGT+GGG + chr8.1:75832543-75832562 None:intergenic 45.0%
GTTGGGATTGCAACTGAAGA+AGG + chr8.1:75833420-75833439 None:intergenic 45.0%
TACTACAATAGCAGCACCTG+TGG - chr8.1:75833901-75833920 MS.gene060491:CDS 45.0%
TATCCTTCTGTTGGAGCTTC+TGG - chr8.1:75833246-75833265 MS.gene060491:intron 45.0%
TCCTGGTCATTGATGTGTGT+GGG - chr8.1:75832852-75832871 MS.gene060491:intron 45.0%
! AAGGTGGTAGCTTTTAGCCT+AGG + chr8.1:75832296-75832315 None:intergenic 45.0%
! CAGTTTTCCATCAACCCACT+AGG - chr8.1:75832526-75832545 MS.gene060491:intron 45.0%
! CCAGTTTTGCCTCAGCATTT+TGG - chr8.1:75833372-75833391 MS.gene060491:intron 45.0%
! GCACAAAGTATCCATGGTCA+TGG - chr8.1:75833315-75833334 MS.gene060491:intron 45.0%
! TGTGGTGGAGATGCAAGTTA+TGG - chr8.1:75832883-75832902 MS.gene060491:intron 45.0%
!! AGTGGGTTGATGGAAAACTG+TGG + chr8.1:75832526-75832545 None:intergenic 45.0%
!! GGTTAGCTTGGGAAACTCAA+AGG - chr8.1:75831709-75831728 MS.gene060491:intron 45.0%
!!! CTTTTTTTTATAAAAAAAAA+AGG - chr8.1:75832203-75832222 MS.gene060491:CDS 5.0%
!!! CTTTTTTTTTTATAAAAAAA+AGG + chr8.1:75832205-75832224 None:intergenic 5.0%
!!! TTTATTTACTATTTTTTTTT+TGG - chr8.1:75832355-75832374 MS.gene060491:CDS 5.0%
!!! TTTTTTTGTTTTATTTTTAT+AGG - chr8.1:75831961-75831980 MS.gene060491:intron 5.0%
AGCACCTGTGGCAGTAACAA+GGG - chr8.1:75833913-75833932 MS.gene060491:CDS 50.0%
CACCAATGGCACCAAAGCTA+TGG - chr8.1:75832906-75832925 MS.gene060491:intron 50.0%
CCTGGTCATTGATGTGTGTG+GGG - chr8.1:75832853-75832872 MS.gene060491:intron 50.0%
CCTTGTGACCCAAAATGCTG+AGG + chr8.1:75833384-75833403 None:intergenic 50.0%
GATTGTACCACTCCATGTGG+AGG + chr8.1:75832480-75832499 None:intergenic 50.0%
GCAATCCCAACCATTAGTGC+TGG - chr8.1:75833429-75833448 MS.gene060491:intron 50.0%
TCCTTCTGTTGGAGCTTCTG+GGG - chr8.1:75833248-75833267 MS.gene060491:intron 50.0%
TCTGTTCCAGAGGCTTGTGA+AGG + chr8.1:75833944-75833963 None:intergenic 50.0%
!! CCTCAGCATTTTGGGTCACA+AGG - chr8.1:75833381-75833400 MS.gene060491:intron 50.0%
!! GCACTTTGGTGCCATAGCTT+TGG + chr8.1:75832920-75832939 None:intergenic 50.0%
!! TGCCATAGCTTTGGTGCCAT+TGG + chr8.1:75832911-75832930 None:intergenic 50.0%
!! TGGTGCCATTGGAGAAATCC+TGG - chr8.1:75832835-75832854 MS.gene060491:intron 50.0%
!! TGTACTGCCTAGTGGGTTGA+TGG + chr8.1:75832536-75832555 None:intergenic 50.0%
!! TTTGGTGCCATTGGTGCCAT+TGG - chr8.1:75832824-75832843 MS.gene060491:intron 50.0%
CAGCACCTGTGGCAGTAACA+AGG - chr8.1:75833912-75833931 MS.gene060491:CDS 55.0%
CCCCACACACATCAATGACC+AGG + chr8.1:75832856-75832875 None:intergenic 55.0%
GGAAGGTTTAGGCCTCCACA+TGG - chr8.1:75832465-75832484 MS.gene060491:intron 55.0%
GTTTAGGCCTCCACATGGAG+TGG - chr8.1:75832470-75832489 MS.gene060491:intron 55.0%
TGTGTGTGGGGATGTGTGTG+TGG - chr8.1:75832865-75832884 MS.gene060491:intron 55.0%
!! AGCTTCTGGGGCAATGGGTT+TGG - chr8.1:75833260-75833279 MS.gene060491:intron 55.0%
!! GTTGGAGCTTCTGGGGCAAT+GGG - chr8.1:75833255-75833274 MS.gene060491:intron 55.0%
!! TGTTGGAGCTTCTGGGGCAA+TGG - chr8.1:75833254-75833273 MS.gene060491:intron 55.0%
CCAGCCCTTGTTACTGCCAC+AGG + chr8.1:75833920-75833939 None:intergenic 60.0%
CCTGTGGCAGTAACAAGGGC+TGG - chr8.1:75833917-75833936 MS.gene060491:CDS 60.0%
GCCCCAGAAGCTCCAACAGA+AGG + chr8.1:75833252-75833271 None:intergenic 60.0%
GCTGGTCCTTCACAAGCCTC+TGG - chr8.1:75833935-75833954 MS.gene060491:CDS 60.0%
GTGTGGGGATGTGTGTGTGG+TGG - chr8.1:75832868-75832887 MS.gene060491:intron 60.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 75831621 75833991 75831621 ID=MS.gene060491
chr8.1 mRNA 75831621 75833991 75831621 ID=MS.gene060491.t1;Parent=MS.gene060491
chr8.1 exon 75833785 75833991 75833785 ID=MS.gene060491.t1.exon1;Parent=MS.gene060491.t1
chr8.1 CDS 75833785 75833991 75833785 ID=cds.MS.gene060491.t1;Parent=MS.gene060491.t1
chr8.1 exon 75833497 75833629 75833497 ID=MS.gene060491.t1.exon2;Parent=MS.gene060491.t1
chr8.1 CDS 75833497 75833629 75833497 ID=cds.MS.gene060491.t1;Parent=MS.gene060491.t1
chr8.1 exon 75833035 75833146 75833035 ID=MS.gene060491.t1.exon3;Parent=MS.gene060491.t1
chr8.1 CDS 75833035 75833146 75833035 ID=cds.MS.gene060491.t1;Parent=MS.gene060491.t1
chr8.1 exon 75832886 75832922 75832886 ID=MS.gene060491.t1.exon4;Parent=MS.gene060491.t1
chr8.1 CDS 75832886 75832922 75832886 ID=cds.MS.gene060491.t1;Parent=MS.gene060491.t1
chr8.1 exon 75832162 75832408 75832162 ID=MS.gene060491.t1.exon5;Parent=MS.gene060491.t1
chr8.1 CDS 75832162 75832408 75832162 ID=cds.MS.gene060491.t1;Parent=MS.gene060491.t1
chr8.1 exon 75831621 75831727 75831621 ID=MS.gene060491.t1.exon6;Parent=MS.gene060491.t1
chr8.1 CDS 75831621 75831727 75831621 ID=cds.MS.gene060491.t1;Parent=MS.gene060491.t1
Gene Sequence

>MS.gene060491

ATGTCTCAACAAAGACAACCTCATATGGTGAGTGGTAACAATCAAGACCAATATAATGACACAACATTTACCAAAATATTTGTTGGAGGGTTAGCTTGGGAAACTCAAAGGGAAACAATGAGAATGTATTTTGAACAATTTGGAGAGATCTTAGAAGCTGTTGTTATCACTGATAAGTATACTGGGAGATCTAAAGGTTATGGTTTTGTAAGTCCATTTTTCTTTTCATAATATTTATTTTACTATGCTTCTCACATTTTTGCATAGTTTATATTTACTTATATATTCCATTTGTGGCTATATATATTTATTTATATGATCTTAGACAATGATTTTGCAATTTTTTTGTTTTATTTTTATAGGTGACATTTAAGGATCCAGAAGCAGCTATAAGAGCATGTCAAAATCCATCTCCAGTTATTGATGGACGAAGAGCAAATTGCAATATTGCTTCTCTTGGTGCTAACAAGAATCGATCACTAACTCTTCAGCATGGTTTTAATCCTTCTACCATTCTCTTTTATTTCTTTCTTTTTTTTTTTTTTACTTGTTATACTATGATCAAATTTATAGTAAAATATCCTTTTTTTTATAAAAAAAAAAGGGTATATTGTGTTATTTATTTGGTTTTAAATTTTCCCTGCCATTGTTCAACCTATCAATTTGATTCATCCTAGGCTAAAAGCTACCACCTTTTTTTTATTAAAGAAACTACCACTTTTTATGGTTGATTATTTATTTACTATTTTTTTTTTGGTACAAATTTACTAATTTATTTGTTGAAAAAAAAATATATGATTATATGTTTTATAAGATTTTAAAGGAAAAATTGAAATGGTGTGCAGGAAGGTTTAGGCCTCCACATGGAGTGGTACAATCAGTTCCTTATCATGGTTCTTCATCCACAGTTTTCCATCAACCCACTAGGCAGTACACATTTCCTTATTCAGCATATGGGTAACTTTTATGTTATGAATTTTTTTATGTTGAGCTAGTTATTTTTTATGAACGTTTGTATATTCTTCGTCTGTCCTTTTCTTTGATGTCAATTATGATATTTATCTGACAGATATTCTGGATATTCACAAGACACATTGTATCCTGCGGTAAGTGTTGATGTAATAAATTCCACATGTTTCAATTAATAGTGAAATCGGCAGAATCACGATGACACAATAATGGGAAATACTTCCATGACATAGTTTTGGTGCCATTGGTGCCATTGGAGAAATCCTGGTCATTGATGTGTGTGGGGATGTGTGTGTGGTGGAGATGCAAGTTATGGCACCAATGGCACCAAAGCTATGGCACCAAAGTGCTACTCATAATTTTGTCAAAATCATGTTGGTTCATCGTAATTTTGTAAAATTGACAGTCAATCCAATATATATGTGATTAATTTGAAAGAATCATTATATCATCGTTATTTTGACAAAAGTTATATTTTAAAGAATCAACTAAATCACGGTTGCATCGTCATTTCATAGTGATTCAAACATACTTAGTAGAAGTAAACTTATAAGTTATATATTTTGATGCATTATTATTTATTGTTGTTTACACTTTTGTGTAAACTATTGCAGAACTATTACAATGTCTATGGAGTTCAACAATATTCACCTTACTATCCTTCTGTTGGAGCTTCTGGGGCAATGGGTTTGGTCCAAAATATGTACCCTTACTATGGTCAATATGCACAAAGTATCCATGGTCATGGTTTTGGAGTTCAATACCCCCAAATGACCCAAATTCCAGTTTTGCCTCAGCATTTTGGGTCACAAGGAATATTGACTCTTCCTTCTTCAGTTGCAATCCCAACCATTAGTGCTGGTAATAATTCTAATACATTATGATATTATTTTTAAATTTTTATAATTTCTCTTCATTGTTGAGCAATTCTATTTCAAAATGGAGTTAGAATCACTAATTAATTTAGTATCAAACTAGGCATATAAAATTTGATCACGAAATTTCAAAATGATTCATTTAGTAAAAAACGAAAGAATAGTTCATTATGTATTCGTTGTAAGGCTGCTTATTTAAAATATATATATATATAGACACACACACACACTTAGAAACTAAATCGACCCACCTGAAAAGACTAATATGATCAAGAATTTTCAATTTTAAGAATTGTTTTGAAACTTCATTAATATCAAATATCAATTTATCTTGCCTCTATAATTTGAGAGATTAAACTAGTGATTTAATTATTTTAAATTTTGTATTGAAGACTAAATTGTAACAATTTTCAGCAACAGTAACTGCAACCACAACTACTACAATAGCAGCACCTGTGGCAGTAACAAGGGCTGGTCCTTCACAAGCCTCTGGAACAGATACTGAACAACAACATTCTACATCTTGA
Protein sequence

>MS.gene060491.t1

MSQQRQPHMVSGNNQDQYNDTTFTKIFVGGLAWETQRETMRMYFEQFGEILEAVVITDKYTGRSKGYGFVTFKDPEAAIRACQNPSPVIDGRRANCNIASLGANKNRSLTLQHGRFRPPHGVVQSVPYHGSSSTVFHQPTRQYTFPYSAYGYSGYSQDTLYPANYYNVYGVQQYSPYYPSVGASGAMGLVQNMYPYYGQYAQSIHGHGFGVQYPQMTQIPVLPQHFGSQGILTLPSSVAIPTISAVTATTTTTIAAPVAVTRAGPSQASGTDTEQQHSTS