Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08915.t1 | XP_013446514.1 | 96.8 | 278 | 9 | 0 | 1 | 278 | 159 | 436 | 3.30E-151 | 544.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08915.t1 | A0A2P1GIW7 | 32.6 | 267 | 144 | 7 | 1 | 258 | 165 | 404 | 5.1e-31 | 136.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08915.t1 | A0A072TTM6 | 96.8 | 278 | 9 | 0 | 1 | 278 | 159 | 436 | 2.4e-151 | 544.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05240 | MS.gene08915 | 0.805748 | 1.15E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 59 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACTCACATTCTGGGAAATT+TGG | 0.164798 | 4.4:-16553568 | None:intergenic |
| CCAATGTATGAAGCTGATTT+TGG | 0.192287 | 4.4:+16554063 | MS.gene08915:CDS |
| TCTATTACAAACTCACATTC+TGG | 0.246799 | 4.4:-16553577 | None:intergenic |
| TTCTCCTTCGCTATTCAATT+TGG | 0.251393 | 4.4:-16553467 | None:intergenic |
| TCTTTCCCTGCAAATTTCTT+TGG | 0.253467 | 4.4:-16553934 | None:intergenic |
| ATTTGTGTTTGAAGCTTCTA+AGG | 0.271551 | 4.4:+16553621 | MS.gene08915:CDS |
| AGCTTCAAACACAAATCTTT+TGG | 0.272043 | 4.4:-16553614 | None:intergenic |
| CTTTCAACTTCATGAATGAT+TGG | 0.304970 | 4.4:+16553443 | MS.gene08915:CDS |
| TTGGATGGGGAAAACCGATA+TGG | 0.308270 | 4.4:+16554082 | MS.gene08915:CDS |
| AATACCAAGTCATGTAGTTC+CGG | 0.332848 | 4.4:-16553871 | None:intergenic |
| ACAAGTTACTACACCGGTTT+CGG | 0.350311 | 4.4:+16553990 | MS.gene08915:CDS |
| GTTTATCCAAAGAAATTTGC+AGG | 0.360964 | 4.4:+16553928 | MS.gene08915:CDS |
| TAAGTCCATGATGGTAAATC+TGG | 0.361121 | 4.4:+16553654 | MS.gene08915:CDS |
| AGTGTGTAGGGAACATAGTT+TGG | 0.402810 | 4.4:+16553830 | MS.gene08915:CDS |
| TTTCAACTTCATGAATGATT+GGG | 0.416199 | 4.4:+16553444 | MS.gene08915:CDS |
| ATACCAAGTCATGTAGTTCC+GGG | 0.423875 | 4.4:-16553870 | None:intergenic |
| GTTTGATTGTTTATGCTAGT+TGG | 0.451953 | 4.4:+16554031 | MS.gene08915:CDS |
| CTATTACAAACTCACATTCT+GGG | 0.456233 | 4.4:-16553576 | None:intergenic |
| TGGATGGGGAAAACCGATAT+GGG | 0.462617 | 4.4:+16554083 | MS.gene08915:CDS |
| TCCTGCATGAAGTAAATTGT+AGG | 0.464671 | 4.4:-16553518 | None:intergenic |
| AAAACTATTGCATTCTTCAC+AGG | 0.486117 | 4.4:-16554123 | None:intergenic |
| GAAATTTGCAGGGAAAGAAA+AGG | 0.492493 | 4.4:+16553939 | MS.gene08915:CDS |
| CACTGTCTGATAAGTGTGTA+GGG | 0.492717 | 4.4:+16553818 | MS.gene08915:CDS |
| TTATAATCACTCTCCGAAAC+CGG | 0.495695 | 4.4:-16554003 | None:intergenic |
| CCAAAATCAGCTTCATACAT+TGG | 0.497579 | 4.4:-16554063 | None:intergenic |
| GTACTCGTTGTCACCCATAT+CGG | 0.503047 | 4.4:-16554096 | None:intergenic |
| TAAGTGTGTGGTTTCTGCAT+TGG | 0.503085 | 4.4:+16553732 | MS.gene08915:CDS |
| CCCTACAATTTACTTCATGC+AGG | 0.509113 | 4.4:+16553517 | MS.gene08915:CDS |
| CAACCCGGAACTACATGACT+TGG | 0.515523 | 4.4:+16553867 | MS.gene08915:CDS |
| CAAAACGACATCGCTTCGCA+TGG | 0.522509 | 4.4:+16553765 | MS.gene08915:CDS |
| GGTTAGATTTGAACGAGAAG+TGG | 0.532468 | 4.4:+16554206 | MS.gene08915:CDS |
| ATGGATACAAGAAATGGAGA+TGG | 0.538458 | 4.4:+16554147 | MS.gene08915:CDS |
| GACATCGCTTCGCATGGCTG+TGG | 0.547958 | 4.4:+16553771 | MS.gene08915:CDS |
| CCTGCATGAAGTAAATTGTA+GGG | 0.557210 | 4.4:-16553517 | None:intergenic |
| CAAAATTATAGACGCTGCAA+CGG | 0.559345 | 4.4:+16553420 | None:intergenic |
| TGTGGATCTTCGTAGAAGAA+TGG | 0.565376 | 4.4:+16553789 | MS.gene08915:CDS |
| CCACTGTCTGATAAGTGTGT+AGG | 0.568598 | 4.4:+16553817 | MS.gene08915:CDS |
| TGAATCCAGATTTACCATCA+TGG | 0.569462 | 4.4:-16553659 | None:intergenic |
| CTTAAAACAAGTTACTACAC+CGG | 0.579115 | 4.4:+16553984 | MS.gene08915:CDS |
| AAAGTTACTTAAGTCCATGA+TGG | 0.580203 | 4.4:+16553645 | MS.gene08915:CDS |
| TTTATCCAAAGAAATTTGCA+GGG | 0.591630 | 4.4:+16553929 | MS.gene08915:CDS |
| ACATCATATCAAACAATACT+CGG | 0.595620 | 4.4:-16554255 | None:intergenic |
| TGCAATGATTTATAAGTGTG+TGG | 0.599319 | 4.4:+16553720 | MS.gene08915:CDS |
| AGTGACAACTTCTACACGTG+TGG | 0.613702 | 4.4:-16553698 | None:intergenic |
| GTTCTTGTTTGTTATCAACC+CGG | 0.616177 | 4.4:+16553852 | MS.gene08915:CDS |
| AACAATACTCGGATTAAGAG+AGG | 0.618426 | 4.4:-16554244 | None:intergenic |
| TGGGCCAAATTGAATAGCGA+AGG | 0.623888 | 4.4:+16553463 | MS.gene08915:CDS |
| TTGGTATTCAAAATTAGACA+AGG | 0.635078 | 4.4:+16553886 | MS.gene08915:CDS |
| AAATTTGGCAAACTCCCTCG+TGG | 0.644808 | 4.4:-16553553 | None:intergenic |
| AATTTGGCAAACTCCCTCGT+GGG | 0.645915 | 4.4:-16553552 | None:intergenic |
| GTGACAACTTCTACACGTGT+GGG | 0.646828 | 4.4:-16553697 | None:intergenic |
| TGGTATTCAAAATTAGACAA+GGG | 0.652202 | 4.4:+16553887 | MS.gene08915:CDS |
| GATTAAGAGAGGCATACTGA+AGG | 0.662777 | 4.4:-16554233 | None:intergenic |
| CCTACACACTTATCAGACAG+TGG | 0.701128 | 4.4:-16553817 | None:intergenic |
| GGTAAATCTGGATTCACATG+AGG | 0.704642 | 4.4:+16553666 | MS.gene08915:CDS |
| GAGATACAATATTCCCACGA+GGG | 0.719170 | 4.4:+16553539 | MS.gene08915:CDS |
| ACACACTTATCAGACAGTGG+AGG | 0.730201 | 4.4:-16553814 | None:intergenic |
| GGAGATACAATATTCCCACG+AGG | 0.760478 | 4.4:+16553538 | MS.gene08915:CDS |
| GAATATGAAAGAGAGTGACA+TGG | 0.783909 | 4.4:+16554185 | MS.gene08915:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGGTATTCAAAATTAGACAA+GGG | + | chr4.4:16553887-16553906 | MS.gene08915:CDS | 25.0% |
| ! | TTGGTATTCAAAATTAGACA+AGG | + | chr4.4:16553886-16553905 | MS.gene08915:CDS | 25.0% |
| ! | TTTATCCAAAGAAATTTGCA+GGG | + | chr4.4:16553929-16553948 | MS.gene08915:CDS | 25.0% |
| ! | TTTCAACTTCATGAATGATT+GGG | + | chr4.4:16553444-16553463 | MS.gene08915:CDS | 25.0% |
| AAAACTATTGCATTCTTCAC+AGG | - | chr4.4:16554126-16554145 | None:intergenic | 30.0% | |
| AAAGTTACTTAAGTCCATGA+TGG | + | chr4.4:16553645-16553664 | MS.gene08915:CDS | 30.0% | |
| CTATTACAAACTCACATTCT+GGG | - | chr4.4:16553579-16553598 | None:intergenic | 30.0% | |
| CTTAAAACAAGTTACTACAC+CGG | + | chr4.4:16553984-16554003 | MS.gene08915:CDS | 30.0% | |
| CTTTCAACTTCATGAATGAT+TGG | + | chr4.4:16553443-16553462 | MS.gene08915:CDS | 30.0% | |
| GTTTATCCAAAGAAATTTGC+AGG | + | chr4.4:16553928-16553947 | MS.gene08915:CDS | 30.0% | |
| GTTTGATTGTTTATGCTAGT+TGG | + | chr4.4:16554031-16554050 | MS.gene08915:CDS | 30.0% | |
| TCTATTACAAACTCACATTC+TGG | - | chr4.4:16553580-16553599 | None:intergenic | 30.0% | |
| TGCAATGATTTATAAGTGTG+TGG | + | chr4.4:16553720-16553739 | MS.gene08915:CDS | 30.0% | |
| ! | AGCTTCAAACACAAATCTTT+TGG | - | chr4.4:16553617-16553636 | None:intergenic | 30.0% |
| ! | ATTTGTGTTTGAAGCTTCTA+AGG | + | chr4.4:16553621-16553640 | MS.gene08915:CDS | 30.0% |
| !! | GTTTTGATGGATACAAGAAA+TGG | + | chr4.4:16554141-16554160 | MS.gene08915:CDS | 30.0% |
| !!! | GAAGAATGCAATAGTTTTGA+TGG | + | chr4.4:16554128-16554147 | MS.gene08915:CDS | 30.0% |
| AACAATACTCGGATTAAGAG+AGG | - | chr4.4:16554247-16554266 | None:intergenic | 35.0% | |
| AATACCAAGTCATGTAGTTC+CGG | - | chr4.4:16553874-16553893 | None:intergenic | 35.0% | |
| ATGGATACAAGAAATGGAGA+TGG | + | chr4.4:16554147-16554166 | MS.gene08915:CDS | 35.0% | |
| CCAAAATCAGCTTCATACAT+TGG | - | chr4.4:16554066-16554085 | None:intergenic | 35.0% | |
| CCTGCATGAAGTAAATTGTA+GGG | - | chr4.4:16553520-16553539 | None:intergenic | 35.0% | |
| GAAATTTGCAGGGAAAGAAA+AGG | + | chr4.4:16553939-16553958 | MS.gene08915:CDS | 35.0% | |
| GAATATGAAAGAGAGTGACA+TGG | + | chr4.4:16554185-16554204 | MS.gene08915:CDS | 35.0% | |
| TAAGTCCATGATGGTAAATC+TGG | + | chr4.4:16553654-16553673 | MS.gene08915:CDS | 35.0% | |
| TCCTGCATGAAGTAAATTGT+AGG | - | chr4.4:16553521-16553540 | None:intergenic | 35.0% | |
| TCTTTCCCTGCAAATTTCTT+TGG | - | chr4.4:16553937-16553956 | None:intergenic | 35.0% | |
| TGAATCCAGATTTACCATCA+TGG | - | chr4.4:16553662-16553681 | None:intergenic | 35.0% | |
| TTATAATCACTCTCCGAAAC+CGG | - | chr4.4:16554006-16554025 | None:intergenic | 35.0% | |
| TTCTCCTTCGCTATTCAATT+TGG | - | chr4.4:16553470-16553489 | None:intergenic | 35.0% | |
| ! | AACTCACATTCTGGGAAATT+TGG | - | chr4.4:16553571-16553590 | None:intergenic | 35.0% |
| !! | CCAATGTATGAAGCTGATTT+TGG | + | chr4.4:16554063-16554082 | MS.gene08915:CDS | 35.0% |
| !! | GTATGAAGCTGATTTTGGAT+GGG | + | chr4.4:16554068-16554087 | MS.gene08915:CDS | 35.0% |
| !! | GTTCTTGTTTGTTATCAACC+CGG | + | chr4.4:16553852-16553871 | MS.gene08915:CDS | 35.0% |
| !! | TATGAAGCTGATTTTGGATG+GGG | + | chr4.4:16554069-16554088 | MS.gene08915:CDS | 35.0% |
| !! | TGTATGAAGCTGATTTTGGA+TGG | + | chr4.4:16554067-16554086 | MS.gene08915:CDS | 35.0% |
| AGTGTGTAGGGAACATAGTT+TGG | + | chr4.4:16553830-16553849 | MS.gene08915:CDS | 40.0% | |
| ATACCAAGTCATGTAGTTCC+GGG | - | chr4.4:16553873-16553892 | None:intergenic | 40.0% | |
| CACTGTCTGATAAGTGTGTA+GGG | + | chr4.4:16553818-16553837 | MS.gene08915:CDS | 40.0% | |
| CCCTACAATTTACTTCATGC+AGG | + | chr4.4:16553517-16553536 | MS.gene08915:CDS | 40.0% | |
| GAGATACAATATTCCCACGA+GGG | + | chr4.4:16553539-16553558 | MS.gene08915:CDS | 40.0% | |
| GGTAAATCTGGATTCACATG+AGG | + | chr4.4:16553666-16553685 | MS.gene08915:CDS | 40.0% | |
| GGTTAGATTTGAACGAGAAG+TGG | + | chr4.4:16554206-16554225 | MS.gene08915:CDS | 40.0% | |
| TAAGTGTGTGGTTTCTGCAT+TGG | + | chr4.4:16553732-16553751 | MS.gene08915:CDS | 40.0% | |
| TGTGGATCTTCGTAGAAGAA+TGG | + | chr4.4:16553789-16553808 | MS.gene08915:CDS | 40.0% | |
| ! | GATTAAGAGAGGCATACTGA+AGG | - | chr4.4:16554236-16554255 | None:intergenic | 40.0% |
| !! | ACAAGTTACTACACCGGTTT+CGG | + | chr4.4:16553990-16554009 | MS.gene08915:CDS | 40.0% |
| AAATTTGGCAAACTCCCTCG+TGG | - | chr4.4:16553556-16553575 | None:intergenic | 45.0% | |
| AATTTGGCAAACTCCCTCGT+GGG | - | chr4.4:16553555-16553574 | None:intergenic | 45.0% | |
| ACACACTTATCAGACAGTGG+AGG | - | chr4.4:16553817-16553836 | None:intergenic | 45.0% | |
| AGTGACAACTTCTACACGTG+TGG | - | chr4.4:16553701-16553720 | None:intergenic | 45.0% | |
| CCACTGTCTGATAAGTGTGT+AGG | + | chr4.4:16553817-16553836 | MS.gene08915:CDS | 45.0% | |
| CCTACACACTTATCAGACAG+TGG | - | chr4.4:16553820-16553839 | None:intergenic | 45.0% | |
| GGAGATACAATATTCCCACG+AGG | + | chr4.4:16553538-16553557 | MS.gene08915:CDS | 45.0% | |
| GTACTCGTTGTCACCCATAT+CGG | - | chr4.4:16554099-16554118 | None:intergenic | 45.0% | |
| GTGACAACTTCTACACGTGT+GGG | - | chr4.4:16553700-16553719 | None:intergenic | 45.0% | |
| TGGATGGGGAAAACCGATAT+GGG | + | chr4.4:16554083-16554102 | MS.gene08915:CDS | 45.0% | |
| TGGGCCAAATTGAATAGCGA+AGG | + | chr4.4:16553463-16553482 | MS.gene08915:CDS | 45.0% | |
| TTGGATGGGGAAAACCGATA+TGG | + | chr4.4:16554082-16554101 | MS.gene08915:CDS | 45.0% | |
| !! | AGGGTTGTGTGAGTTTTGTG+AGG | + | chr4.4:16553906-16553925 | MS.gene08915:CDS | 45.0% |
| CAAAACGACATCGCTTCGCA+TGG | + | chr4.4:16553765-16553784 | MS.gene08915:CDS | 50.0% | |
| CAACCCGGAACTACATGACT+TGG | + | chr4.4:16553867-16553886 | MS.gene08915:CDS | 50.0% | |
| GACATCGCTTCGCATGGCTG+TGG | + | chr4.4:16553771-16553790 | MS.gene08915:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 16553431 | 16554269 | 16553431 | ID=MS.gene08915 |
| chr4.4 | mRNA | 16553431 | 16554269 | 16553431 | ID=MS.gene08915.t1;Parent=MS.gene08915 |
| chr4.4 | exon | 16553431 | 16554269 | 16553431 | ID=MS.gene08915.t1.exon1;Parent=MS.gene08915.t1 |
| chr4.4 | CDS | 16553431 | 16554269 | 16553431 | ID=cds.MS.gene08915.t1;Parent=MS.gene08915.t1 |
| Gene Sequence |
| Protein sequence |
