Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09015.t1 | XP_003630495.1 | 76.3 | 236 | 55 | 1 | 1 | 236 | 1 | 235 | 5.30E-81 | 310.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09015.t1 | G7LHA8 | 76.3 | 236 | 55 | 1 | 1 | 236 | 1 | 235 | 3.8e-81 | 310.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053226 | MS.gene09015 | 0.802842 | 4.63E-49 | -1.69E-46 |
| MS.gene05689 | MS.gene09015 | 0.81498 | 1.17E-51 | -1.69E-46 |
| MS.gene059935 | MS.gene09015 | 0.820692 | 6.00E-53 | -1.69E-46 |
| MS.gene060365 | MS.gene09015 | 0.814754 | 1.31E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09015.t1 | MTR_8g097140 | 86.864 | 236 | 30 | 1 | 1 | 236 | 1 | 235 | 9.33e-140 | 391 |
| MS.gene09015.t1 | MTR_1g016270 | 49.810 | 263 | 83 | 7 | 3 | 221 | 4 | 261 | 1.75e-60 | 191 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 53 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACAAAATTGGAGAAAATTT+TGG | 0.213621 | 4.4:-15217295 | None:intergenic |
| GGAGGCTATGATGTTATTCT+TGG | 0.292173 | 4.4:+15217339 | MS.gene09015:CDS |
| TCTGCTGACAATGGTGCTTC+AGG | 0.313597 | 4.4:+15217318 | MS.gene09015:CDS |
| AAAGAGTTTCGCAATGTTGA+TGG | 0.379797 | 4.4:+15217483 | MS.gene09015:CDS |
| GATTCACAACGACCGGTAAT+CGG | 0.388566 | 4.4:-15217243 | None:intergenic |
| CGAAATTCGATGCCGATTAC+CGG | 0.390204 | 4.4:+15217231 | None:intergenic |
| TGGAGGGCCGATGGGTCCTC+GGG | 0.410669 | 4.4:+15217776 | MS.gene09015:CDS |
| AACTATATGCTGCGGAGAAA+AGG | 0.421356 | 4.4:+15217847 | MS.gene09015:CDS |
| GTTTAAGTTCCTTGTGAAGC+TGG | 0.424424 | 4.4:-15217392 | None:intergenic |
| GAGAGAATTGAAAGTAGGTC+GGG | 0.434501 | 4.4:+15217897 | MS.gene09015:CDS |
| GTCCTCGGGACATAGAAATT+AGG | 0.434748 | 4.4:+15217790 | MS.gene09015:CDS |
| AAATTCAATCAAGAAGTTAG+TGG | 0.438478 | 4.4:+15217528 | MS.gene09015:CDS |
| GTGGAGGGCCGATGGGTCCT+CGG | 0.438544 | 4.4:+15217775 | MS.gene09015:CDS |
| ATTGTCAGCAGAGACAAAAT+TGG | 0.442188 | 4.4:-15217307 | None:intergenic |
| TTAAACTATCAAAATCTCAT+AGG | 0.445350 | 4.4:+15217409 | MS.gene09015:CDS |
| CGGAGGGTTAGTTGCTCCTC+GGG | 0.449386 | 4.4:+15217608 | MS.gene09015:CDS |
| CAACACTATCTTCTGTTATG+TGG | 0.484368 | 4.4:-15217446 | None:intergenic |
| TATTCTTGGAAAGACTTTCA+AGG | 0.486432 | 4.4:+15217353 | MS.gene09015:CDS |
| GTGAAGAGAGAATTGAAAGT+AGG | 0.506842 | 4.4:+15217892 | MS.gene09015:CDS |
| TTTAGATGATTCACAACGAC+CGG | 0.509528 | 4.4:-15217250 | None:intergenic |
| AGAGAGAATTGAAAGTAGGT+CGG | 0.510153 | 4.4:+15217896 | MS.gene09015:CDS |
| AAGAGTTTCGCAATGTTGAT+GGG | 0.523006 | 4.4:+15217484 | MS.gene09015:CDS |
| ATTCTTGGAAAGACTTTCAA+GGG | 0.523914 | 4.4:+15217354 | MS.gene09015:CDS |
| GGGACAAGAACAACAGAAAG+AGG | 0.527695 | 4.4:+15217722 | MS.gene09015:CDS |
| CTCCTCGGGATGTAGAAGTC+AGG | 0.538589 | 4.4:+15217622 | MS.gene09015:CDS |
| TTTAGAAGTTGAAAATGCGA+AGG | 0.547721 | 4.4:+15217701 | MS.gene09015:CDS |
| ACAAACAAGAAACTTGAAGC+AGG | 0.557290 | 4.4:+15217813 | MS.gene09015:CDS |
| GCGGAGGGTTAGTTGCTCCT+CGG | 0.558909 | 4.4:+15217607 | MS.gene09015:CDS |
| AAGAAGAGGAAGAATCAAGA+TGG | 0.562761 | 4.4:+15217870 | MS.gene09015:CDS |
| GAATGAAGCCGTGAGTGAGA+AGG | 0.573910 | 4.4:+15217656 | MS.gene09015:CDS |
| AGCAATGATGTGAAGAGTGG+AGG | 0.578389 | 4.4:+15217759 | MS.gene09015:CDS |
| TAAGAAGAAAATGAAACACT+AGG | 0.578476 | 4.4:+15217929 | MS.gene09015:CDS |
| GCAATGATGTGAAGAGTGGA+GGG | 0.588416 | 4.4:+15217760 | MS.gene09015:CDS |
| GTGAAGAGTGGAGGGCCGAT+GGG | 0.590084 | 4.4:+15217768 | MS.gene09015:CDS |
| TGAGAAGGAGAAAATGCAAA+GGG | 0.591298 | 4.4:+15217671 | MS.gene09015:CDS |
| AAGAAGAAAATGAAACACTA+GGG | 0.592449 | 4.4:+15217930 | MS.gene09015:CDS |
| GTCCTAATTTCTATGTCCCG+AGG | 0.595550 | 4.4:-15217792 | None:intergenic |
| TGTGAAGAGTGGAGGGCCGA+TGG | 0.598004 | 4.4:+15217767 | MS.gene09015:CDS |
| TTCTAAGCAACAGAAAGAGA+TGG | 0.598515 | 4.4:+15217551 | MS.gene09015:CDS |
| CTGCGGAGAAAAGGAAGAAG+AGG | 0.599956 | 4.4:+15217856 | MS.gene09015:CDS |
| CTAAGCAATGATGTGAAGAG+TGG | 0.605046 | 4.4:+15217756 | MS.gene09015:CDS |
| CTTGCTCAACCAGCTTCACA+AGG | 0.605167 | 4.4:+15217383 | MS.gene09015:CDS |
| AAAGATAAGTAACAATGCGA+AGG | 0.605305 | 4.4:+15217578 | MS.gene09015:CDS |
| TCTATGTCCCGAGGACCCAT+CGG | 0.610923 | 4.4:-15217783 | None:intergenic |
| TTAGAAGTTGAAAATGCGAA+GGG | 0.610992 | 4.4:+15217702 | MS.gene09015:CDS |
| GCTGACAATGGTGCTTCAGG+AGG | 0.622331 | 4.4:+15217321 | MS.gene09015:CDS |
| GACCTGACTTCTACATCCCG+AGG | 0.639086 | 4.4:-15217624 | None:intergenic |
| GTGAGAAGGAGAAAATGCAA+AGG | 0.641271 | 4.4:+15217670 | MS.gene09015:CDS |
| AATGCGAAGGTTGAAAGCGG+AGG | 0.641854 | 4.4:+15217591 | MS.gene09015:CDS |
| ATGCGAAGGTTGAAAGCGGA+GGG | 0.642455 | 4.4:+15217592 | MS.gene09015:CDS |
| CAAACAAGAAACTTGAAGCA+GGG | 0.648067 | 4.4:+15217814 | MS.gene09015:CDS |
| AACAATGCGAAGGTTGAAAG+CGG | 0.662379 | 4.4:+15217588 | MS.gene09015:CDS |
| TGAGAGTAAACTATATGCTG+CGG | 0.662602 | 4.4:+15217839 | MS.gene09015:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAAACTATCAAAATCTCAT+AGG | + | chr4.4:15217409-15217428 | MS.gene09015:CDS | 20.0% |
| ! | AAATTCAATCAAGAAGTTAG+TGG | + | chr4.4:15217528-15217547 | MS.gene09015:CDS | 25.0% |
| !! | GACAAAATTGGAGAAAATTT+TGG | - | chr4.4:15217298-15217317 | None:intergenic | 25.0% |
| AAAGATAAGTAACAATGCGA+AGG | + | chr4.4:15217578-15217597 | MS.gene09015:CDS | 30.0% | |
| ATTCTTGGAAAGACTTTCAA+GGG | + | chr4.4:15217354-15217373 | MS.gene09015:CDS | 30.0% | |
| TATTCTTGGAAAGACTTTCA+AGG | + | chr4.4:15217353-15217372 | MS.gene09015:CDS | 30.0% | |
| TTAGAAGTTGAAAATGCGAA+GGG | + | chr4.4:15217702-15217721 | MS.gene09015:CDS | 30.0% | |
| TTTAGAAGTTGAAAATGCGA+AGG | + | chr4.4:15217701-15217720 | MS.gene09015:CDS | 30.0% | |
| AAAGAGTTTCGCAATGTTGA+TGG | + | chr4.4:15217483-15217502 | MS.gene09015:CDS | 35.0% | |
| AAGAAGAGGAAGAATCAAGA+TGG | + | chr4.4:15217870-15217889 | MS.gene09015:CDS | 35.0% | |
| AAGAGTTTCGCAATGTTGAT+GGG | + | chr4.4:15217484-15217503 | MS.gene09015:CDS | 35.0% | |
| ACAAACAAGAAACTTGAAGC+AGG | + | chr4.4:15217813-15217832 | MS.gene09015:CDS | 35.0% | |
| AGAGAGAATTGAAAGTAGGT+CGG | + | chr4.4:15217896-15217915 | MS.gene09015:CDS | 35.0% | |
| ATTGTCAGCAGAGACAAAAT+TGG | - | chr4.4:15217310-15217329 | None:intergenic | 35.0% | |
| CAAACAAGAAACTTGAAGCA+GGG | + | chr4.4:15217814-15217833 | MS.gene09015:CDS | 35.0% | |
| CAACACTATCTTCTGTTATG+TGG | - | chr4.4:15217449-15217468 | None:intergenic | 35.0% | |
| GTGAAGAGAGAATTGAAAGT+AGG | + | chr4.4:15217892-15217911 | MS.gene09015:CDS | 35.0% | |
| TAGAAGTCAGGTCAAAAAAG+AGG | + | chr4.4:15217634-15217653 | MS.gene09015:CDS | 35.0% | |
| TGAGAAGGAGAAAATGCAAA+GGG | + | chr4.4:15217671-15217690 | MS.gene09015:CDS | 35.0% | |
| TGAGAGTAAACTATATGCTG+CGG | + | chr4.4:15217839-15217858 | MS.gene09015:CDS | 35.0% | |
| TTCTAAGCAACAGAAAGAGA+TGG | + | chr4.4:15217551-15217570 | MS.gene09015:CDS | 35.0% | |
| TTTAGATGATTCACAACGAC+CGG | - | chr4.4:15217253-15217272 | None:intergenic | 35.0% | |
| ! | AATTTTGTCTCTGCTGACAA+TGG | + | chr4.4:15217309-15217328 | MS.gene09015:CDS | 35.0% |
| AACAATGCGAAGGTTGAAAG+CGG | + | chr4.4:15217588-15217607 | MS.gene09015:CDS | 40.0% | |
| AACTATATGCTGCGGAGAAA+AGG | + | chr4.4:15217847-15217866 | MS.gene09015:CDS | 40.0% | |
| CTAAGCAATGATGTGAAGAG+TGG | + | chr4.4:15217756-15217775 | MS.gene09015:CDS | 40.0% | |
| GAGAGAATTGAAAGTAGGTC+GGG | + | chr4.4:15217897-15217916 | MS.gene09015:CDS | 40.0% | |
| GGAGGCTATGATGTTATTCT+TGG | + | chr4.4:15217339-15217358 | MS.gene09015:CDS | 40.0% | |
| GTGAGAAGGAGAAAATGCAA+AGG | + | chr4.4:15217670-15217689 | MS.gene09015:CDS | 40.0% | |
| ! | CATTTTCTCCTTCTCACTCA+CGG | - | chr4.4:15217667-15217686 | None:intergenic | 40.0% |
| ! | GTTTAAGTTCCTTGTGAAGC+TGG | - | chr4.4:15217395-15217414 | None:intergenic | 40.0% |
| AGCAATGATGTGAAGAGTGG+AGG | + | chr4.4:15217759-15217778 | MS.gene09015:CDS | 45.0% | |
| GATTCACAACGACCGGTAAT+CGG | - | chr4.4:15217246-15217265 | None:intergenic | 45.0% | |
| GCAATGATGTGAAGAGTGGA+GGG | + | chr4.4:15217760-15217779 | MS.gene09015:CDS | 45.0% | |
| GGGACAAGAACAACAGAAAG+AGG | + | chr4.4:15217722-15217741 | MS.gene09015:CDS | 45.0% | |
| GTCCTAATTTCTATGTCCCG+AGG | - | chr4.4:15217795-15217814 | None:intergenic | 45.0% | |
| GTCCTCGGGACATAGAAATT+AGG | + | chr4.4:15217790-15217809 | MS.gene09015:CDS | 45.0% | |
| !! | GGAGAAAATTTTGGCAGCTC+TGG | - | chr4.4:15217289-15217308 | None:intergenic | 45.0% |
| AATGCGAAGGTTGAAAGCGG+AGG | + | chr4.4:15217591-15217610 | MS.gene09015:CDS | 50.0% | |
| ATGCGAAGGTTGAAAGCGGA+GGG | + | chr4.4:15217592-15217611 | MS.gene09015:CDS | 50.0% | |
| CTGCGGAGAAAAGGAAGAAG+AGG | + | chr4.4:15217856-15217875 | MS.gene09015:CDS | 50.0% | |
| CTTGCTCAACCAGCTTCACA+AGG | + | chr4.4:15217383-15217402 | MS.gene09015:CDS | 50.0% | |
| GAATGAAGCCGTGAGTGAGA+AGG | + | chr4.4:15217656-15217675 | MS.gene09015:CDS | 50.0% | |
| TCTGCTGACAATGGTGCTTC+AGG | + | chr4.4:15217318-15217337 | MS.gene09015:CDS | 50.0% | |
| CTCCTCGGGATGTAGAAGTC+AGG | + | chr4.4:15217622-15217641 | MS.gene09015:CDS | 55.0% | |
| GACCTGACTTCTACATCCCG+AGG | - | chr4.4:15217627-15217646 | None:intergenic | 55.0% | |
| GCTGACAATGGTGCTTCAGG+AGG | + | chr4.4:15217321-15217340 | MS.gene09015:CDS | 55.0% | |
| TCTATGTCCCGAGGACCCAT+CGG | - | chr4.4:15217786-15217805 | None:intergenic | 55.0% | |
| GTGAAGAGTGGAGGGCCGAT+GGG | + | chr4.4:15217768-15217787 | MS.gene09015:CDS | 60.0% | |
| TGTGAAGAGTGGAGGGCCGA+TGG | + | chr4.4:15217767-15217786 | MS.gene09015:CDS | 60.0% | |
| !! | CGGAGGGTTAGTTGCTCCTC+GGG | + | chr4.4:15217608-15217627 | MS.gene09015:CDS | 60.0% |
| !! | GCGGAGGGTTAGTTGCTCCT+CGG | + | chr4.4:15217607-15217626 | MS.gene09015:CDS | 60.0% |
| GTGGAGGGCCGATGGGTCCT+CGG | + | chr4.4:15217775-15217794 | MS.gene09015:CDS | 70.0% | |
| TGGAGGGCCGATGGGTCCTC+GGG | + | chr4.4:15217776-15217795 | MS.gene09015:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 15217240 | 15217950 | 15217240 | ID=MS.gene09015 |
| chr4.4 | mRNA | 15217240 | 15217950 | 15217240 | ID=MS.gene09015.t1;Parent=MS.gene09015 |
| chr4.4 | exon | 15217240 | 15217950 | 15217240 | ID=MS.gene09015.t1.exon1;Parent=MS.gene09015.t1 |
| chr4.4 | CDS | 15217240 | 15217950 | 15217240 | ID=cds.MS.gene09015.t1;Parent=MS.gene09015.t1 |
| Gene Sequence |
| Protein sequence |
