Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09405.t1 | RHN81465.1 | 98.6 | 71 | 1 | 0 | 1 | 71 | 162 | 232 | 1.20E-31 | 145.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09405.t1 | F4IUW3 | 81.7 | 71 | 13 | 0 | 1 | 71 | 170 | 240 | 7.8e-29 | 127.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09405.t1 | A0A072VPC5 | 98.6 | 71 | 1 | 0 | 1 | 71 | 162 | 232 | 8.3e-32 | 145.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057127 | MS.gene09405 | 0.806486 | 8.04E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGCAGCTTGGCCAATATC+AGG | 0.277637 | 1.2:+73875575 | None:intergenic |
| GATATTGGCCAAGCTGCTTT+TGG | 0.282327 | 1.2:-73875572 | MS.gene09405:CDS |
| CCACCTTCTTTAGCAGCATA+AGG | 0.371725 | 1.2:+73875701 | None:intergenic |
| CTTGAAACATACCCTGATAT+TGG | 0.373374 | 1.2:-73875587 | MS.gene09405:CDS |
| CACCTTCTTTAGCAGCATAA+GGG | 0.381981 | 1.2:+73875702 | None:intergenic |
| ATTGCTCTTGCGTTCCTGCT+TGG | 0.419631 | 1.2:-73875624 | MS.gene09405:CDS |
| GCAGGCATAAATGTCCTTTG+TGG | 0.485224 | 1.2:-73875743 | None:intergenic |
| ACCCCTTATGCTGCTAAAGA+AGG | 0.496565 | 1.2:-73875704 | MS.gene09405:CDS |
| TAGCAGCATAAGGGGTTGAA+AGG | 0.507018 | 1.2:+73875711 | None:intergenic |
| ATAAATGTCCTTTGTGGAGT+AGG | 0.510264 | 1.2:-73875737 | MS.gene09405:CDS |
| TACTGAAATAGCAATTCGTC+CGG | 0.520360 | 1.2:+73875544 | None:intergenic |
| ATGCTGCTAAAGAAGGTGGA+TGG | 0.529172 | 1.2:-73875697 | MS.gene09405:CDS |
| TCCTGCTTGGATAGTGAACC+TGG | 0.539558 | 1.2:-73875611 | MS.gene09405:CDS |
| TCAGGGTATGTTTCAAGACC+AGG | 0.543344 | 1.2:+73875593 | None:intergenic |
| CGGCAGTACCAAAAGCAGCT+TGG | 0.551226 | 1.2:+73875564 | None:intergenic |
| ACCTTCTTTAGCAGCATAAG+GGG | 0.553489 | 1.2:+73875703 | None:intergenic |
| TGCTGCTAAAGAAGGTGGAT+GGG | 0.558871 | 1.2:-73875696 | MS.gene09405:CDS |
| GCTAAAGAAGGTGGATGGGT+AGG | 0.573926 | 1.2:-73875692 | MS.gene09405:CDS |
| CCTTATGCTGCTAAAGAAGG+TGG | 0.584559 | 1.2:-73875701 | MS.gene09405:CDS |
| ACCAGGTTCACTATCCAAGC+AGG | 0.585794 | 1.2:+73875610 | None:intergenic |
| AAGCAGCTTGGCCAATATCA+GGG | 0.606674 | 1.2:+73875576 | None:intergenic |
| AAAGGATTCCTACTCCACAA+AGG | 0.721420 | 1.2:+73875729 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTCCAAAAATGAATAAGA+TGG | + | chr1.2:73875625-73875644 | None:intergenic | 20.0% |
| !! | CTTTCCATCTTATTCATTTT+TGG | - | chr1.2:73875618-73875637 | MS.gene09405:CDS | 25.0% |
| !! | GGAATTCTTTCTTTTTATAC+TGG | - | chr1.2:73875639-73875658 | MS.gene09405:CDS | 25.0% |
| ATAAATGTCCTTTGTGGAGT+AGG | - | chr1.2:73875549-73875568 | MS.gene09405:CDS | 35.0% | |
| CTTGAAACATACCCTGATAT+TGG | - | chr1.2:73875699-73875718 | MS.gene09405:CDS | 35.0% | |
| AAAGGATTCCTACTCCACAA+AGG | + | chr1.2:73875560-73875579 | None:intergenic | 40.0% | |
| ACCTTCTTTAGCAGCATAAG+GGG | + | chr1.2:73875586-73875605 | None:intergenic | 40.0% | |
| CACCTTCTTTAGCAGCATAA+GGG | + | chr1.2:73875587-73875606 | None:intergenic | 40.0% | |
| AAAGCAGCTTGGCCAATATC+AGG | + | chr1.2:73875714-73875733 | None:intergenic | 45.0% | |
| AAGCAGCTTGGCCAATATCA+GGG | + | chr1.2:73875713-73875732 | None:intergenic | 45.0% | |
| ACCCCTTATGCTGCTAAAGA+AGG | - | chr1.2:73875582-73875601 | MS.gene09405:CDS | 45.0% | |
| ATGCTGCTAAAGAAGGTGGA+TGG | - | chr1.2:73875589-73875608 | MS.gene09405:CDS | 45.0% | |
| CCACCTTCTTTAGCAGCATA+AGG | + | chr1.2:73875588-73875607 | None:intergenic | 45.0% | |
| CCTTATGCTGCTAAAGAAGG+TGG | - | chr1.2:73875585-73875604 | MS.gene09405:CDS | 45.0% | |
| TAGCAGCATAAGGGGTTGAA+AGG | + | chr1.2:73875578-73875597 | None:intergenic | 45.0% | |
| TCAGGGTATGTTTCAAGACC+AGG | + | chr1.2:73875696-73875715 | None:intergenic | 45.0% | |
| TGCTGCTAAAGAAGGTGGAT+GGG | - | chr1.2:73875590-73875609 | MS.gene09405:CDS | 45.0% | |
| !! | GATATTGGCCAAGCTGCTTT+TGG | - | chr1.2:73875714-73875733 | MS.gene09405:CDS | 45.0% |
| ACCAGGTTCACTATCCAAGC+AGG | + | chr1.2:73875679-73875698 | None:intergenic | 50.0% | |
| ATTGCTCTTGCGTTCCTGCT+TGG | - | chr1.2:73875662-73875681 | MS.gene09405:CDS | 50.0% | |
| GCTAAAGAAGGTGGATGGGT+AGG | - | chr1.2:73875594-73875613 | MS.gene09405:CDS | 50.0% | |
| TCCTGCTTGGATAGTGAACC+TGG | - | chr1.2:73875675-73875694 | MS.gene09405:CDS | 50.0% | |
| !!! | CAAGCTGCTTTTGGTACTGC+CGG | - | chr1.2:73875723-73875742 | MS.gene09405:CDS | 50.0% |
| CGGCAGTACCAAAAGCAGCT+TGG | + | chr1.2:73875725-73875744 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 73875547 | 73875761 | 73875547 | ID=MS.gene09405 |
| chr1.2 | mRNA | 73875547 | 73875761 | 73875547 | ID=MS.gene09405.t1;Parent=MS.gene09405 |
| chr1.2 | exon | 73875547 | 73875761 | 73875547 | ID=MS.gene09405.t1.exon1;Parent=MS.gene09405.t1 |
| chr1.2 | CDS | 73875547 | 73875761 | 73875547 | ID=cds.MS.gene09405.t1;Parent=MS.gene09405.t1 |
| Gene Sequence |
| Protein sequence |
