Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09450.t1 | PNX90420.1 | 63.6 | 258 | 53 | 2 | 6 | 222 | 130 | 387 | 1.50E-75 | 292.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09450.t1 | P42730 | 61.7 | 256 | 57 | 2 | 6 | 220 | 129 | 384 | 9.7e-76 | 284.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09450.t1 | A0A2K3MHZ8 | 63.6 | 258 | 53 | 2 | 6 | 222 | 130 | 387 | 1.1e-75 | 292.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054801 | MS.gene09450 | 0.808103 | 3.65E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09450.t1 | MTR_4g109450 | 63.672 | 256 | 52 | 2 | 6 | 220 | 129 | 384 | 1.88e-96 | 300 |
| MS.gene09450.t1 | MTR_4g109450 | 63.672 | 256 | 52 | 2 | 6 | 220 | 129 | 384 | 5.31e-96 | 300 |
| MS.gene09450.t1 | MTR_7g028970 | 57.031 | 256 | 69 | 2 | 6 | 220 | 129 | 384 | 2.70e-86 | 274 |
| MS.gene09450.t1 | MTR_7g012820 | 45.489 | 266 | 98 | 5 | 1 | 223 | 136 | 397 | 5.03e-64 | 214 |
| MS.gene09450.t1 | MTR_7g012820 | 45.489 | 266 | 98 | 5 | 1 | 223 | 207 | 468 | 6.13e-64 | 214 |
| MS.gene09450.t1 | MTR_6g013660 | 45.113 | 266 | 99 | 5 | 1 | 223 | 203 | 464 | 1.25e-63 | 213 |
| MS.gene09450.t1 | MTR_3g106160 | 48.472 | 229 | 75 | 3 | 38 | 223 | 248 | 476 | 1.99e-61 | 207 |
| MS.gene09450.t1 | MTR_8g100040 | 39.544 | 263 | 111 | 5 | 8 | 223 | 222 | 483 | 7.59e-56 | 191 |
| MS.gene09450.t1 | MTR_3g498725 | 39.163 | 263 | 112 | 5 | 8 | 223 | 218 | 479 | 5.29e-55 | 189 |
| MS.gene09450.t1 | MTR_3g102830 | 39.163 | 263 | 112 | 5 | 8 | 223 | 223 | 484 | 7.47e-55 | 188 |
| MS.gene09450.t1 | MTR_3g461000 | 33.459 | 266 | 126 | 6 | 8 | 223 | 131 | 395 | 3.84e-37 | 138 |
| MS.gene09450.t1 | MTR_3g098310 | 36.207 | 232 | 99 | 5 | 40 | 223 | 297 | 527 | 4.70e-36 | 135 |
| MS.gene09450.t1 | MTR_3g098310 | 36.364 | 231 | 98 | 5 | 41 | 223 | 298 | 527 | 6.35e-36 | 134 |
| MS.gene09450.t1 | MTR_6g048860 | 42.336 | 137 | 59 | 3 | 29 | 146 | 124 | 259 | 5.22e-30 | 114 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09450.t1 | AT1G74310 | 61.719 | 256 | 57 | 2 | 6 | 220 | 129 | 384 | 3.79e-94 | 293 |
| MS.gene09450.t1 | AT1G74310 | 61.719 | 256 | 57 | 2 | 6 | 220 | 129 | 384 | 1.30e-93 | 293 |
| MS.gene09450.t1 | AT4G14670 | 51.562 | 256 | 72 | 3 | 6 | 220 | 105 | 349 | 6.55e-74 | 236 |
| MS.gene09450.t1 | AT5G15450 | 45.489 | 266 | 98 | 5 | 1 | 223 | 201 | 462 | 2.94e-63 | 212 |
| MS.gene09450.t1 | AT2G25140 | 47.414 | 232 | 79 | 3 | 35 | 223 | 236 | 467 | 2.93e-61 | 207 |
| MS.gene09450.t1 | AT5G50920 | 39.313 | 262 | 112 | 5 | 8 | 223 | 221 | 481 | 6.12e-54 | 186 |
| MS.gene09450.t1 | AT3G48870 | 44.053 | 227 | 83 | 4 | 40 | 223 | 246 | 471 | 1.05e-53 | 185 |
| MS.gene09450.t1 | AT3G48870 | 44.053 | 227 | 83 | 4 | 40 | 223 | 277 | 502 | 1.35e-53 | 185 |
| MS.gene09450.t1 | AT3G48870 | 44.053 | 227 | 83 | 4 | 40 | 223 | 277 | 502 | 1.35e-53 | 185 |
| MS.gene09450.t1 | AT3G48870 | 44.053 | 227 | 83 | 4 | 40 | 223 | 277 | 502 | 1.35e-53 | 185 |
| MS.gene09450.t1 | AT5G51070 | 37.931 | 232 | 95 | 4 | 40 | 223 | 270 | 500 | 8.00e-39 | 143 |
| MS.gene09450.t1 | AT3G45450 | 31.068 | 206 | 108 | 6 | 8 | 186 | 111 | 309 | 6.08e-20 | 87.4 |
Find 38 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTGATCACGTCCAATAAT+AGG | 0.203714 | 6.4:-23616879 | None:intergenic |
| TGAAGCATACTAATGGTATT+TGG | 0.257664 | 6.4:-23617397 | None:intergenic |
| AAGAAAGTTGAAAGTGCTTT+TGG | 0.276719 | 6.4:+23616789 | MS.gene09450:CDS |
| AATGAAATCCATCTTGTTCT+TGG | 0.293580 | 6.4:+23617201 | MS.gene09450:CDS |
| AAGCTGGTGTCGATGTTTCT+AGG | 0.301784 | 6.4:+23616727 | MS.gene09450:CDS |
| GGGAAAATAAACCCTATTAT+TGG | 0.311208 | 6.4:+23616867 | MS.gene09450:CDS |
| CTTGATCACGTCCAATAATA+GGG | 0.351376 | 6.4:-23616878 | None:intergenic |
| AAGAACAATCCTATTCTTGT+AGG | 0.358016 | 6.4:+23616939 | MS.gene09450:CDS |
| TATTCTATGAAGCATACTAA+TGG | 0.381436 | 6.4:-23617404 | None:intergenic |
| AGCAATTTGGTTGAGCGAGC+AGG | 0.402181 | 6.4:+23616846 | MS.gene09450:CDS |
| CCTGGCTCTCCTACAAGAAT+AGG | 0.403888 | 6.4:-23616948 | None:intergenic |
| TAACAACAATTGAAGAAAAC+AGG | 0.418285 | 6.4:+23617305 | MS.gene09450:CDS |
| ATGAAATCCATCTTGTTCTT+GGG | 0.442228 | 6.4:+23617202 | MS.gene09450:CDS |
| TAGTTGCTGGTGCTAAGAGC+AGG | 0.449394 | 6.4:+23617103 | MS.gene09450:CDS |
| AAAAGCTTTATCGTAAAGAA+AGG | 0.452039 | 6.4:+23616766 | MS.gene09450:CDS |
| CCTATTCTTGTAGGAGAGCC+AGG | 0.453300 | 6.4:+23616948 | MS.gene09450:CDS |
| GTACTCTAAGGACGGTTGTC+AGG | 0.458575 | 6.4:+23617527 | MS.gene09450:CDS |
| AAGTGGAAGAAGCTGAAGGA+AGG | 0.467536 | 6.4:+23617163 | MS.gene09450:CDS |
| GATATCGGCGCATTAGTTGC+TGG | 0.470023 | 6.4:+23617090 | MS.gene09450:intron |
| AATTCTACCCAAGAACAAGA+TGG | 0.517715 | 6.4:-23617209 | None:intergenic |
| CTAATGGTATTTGGAACACT+AGG | 0.519006 | 6.4:-23617388 | None:intergenic |
| TTGTTGAAGGGTTGGCTCAA+AGG | 0.521528 | 6.4:+23616988 | MS.gene09450:CDS |
| AATTGCTGTTGTTGAAGGGT+TGG | 0.527963 | 6.4:+23616980 | MS.gene09450:CDS |
| GGTGGATATCTTAGATGCAT+TGG | 0.530848 | 6.4:+23617280 | MS.gene09450:intron |
| AGTGGAAGAAGCTGAAGGAA+GGG | 0.534741 | 6.4:+23617164 | MS.gene09450:CDS |
| GACGTGATCAAGAGATAGAA+AGG | 0.537055 | 6.4:+23616889 | MS.gene09450:CDS |
| TTGTCTAGTCAATACATAGC+TGG | 0.543197 | 6.4:+23617493 | MS.gene09450:CDS |
| AAGATGCAGCATTTGAGAGG+AGG | 0.551112 | 6.4:+23617341 | MS.gene09450:CDS |
| GAAGGGTTGGCTCAAAGGAT+AGG | 0.553623 | 6.4:+23616993 | MS.gene09450:CDS |
| AGCTGGTGTCGATGTTTCTA+GGG | 0.557420 | 6.4:+23616728 | MS.gene09450:CDS |
| GCAATTTGGTTGAGCGAGCA+GGG | 0.560164 | 6.4:+23616847 | MS.gene09450:CDS |
| TGTCTTGAATTATAACGTCA+TGG | 0.566735 | 6.4:-23617447 | None:intergenic |
| AAAGAAGTGGAAGAAGCTGA+AGG | 0.582184 | 6.4:+23617159 | MS.gene09450:CDS |
| ATGTACAAAAGCTTTGAAGC+TGG | 0.591312 | 6.4:+23616711 | MS.gene09450:CDS |
| AGTTGCTGGTGCTAAGAGCA+GGG | 0.591817 | 6.4:+23617104 | MS.gene09450:CDS |
| GTTGCTGGTGCTAAGAGCAG+GGG | 0.668987 | 6.4:+23617105 | MS.gene09450:CDS |
| GAGGTTTGTACAGATTGATG+TGG | 0.706203 | 6.4:+23617360 | MS.gene09450:CDS |
| TAGAATAAAAGAGATGTACG+AGG | 0.759659 | 6.4:+23617420 | MS.gene09450:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTCAAGTTTTGAAAAAATA+TGG | + | chr6.4:23616822-23616841 | MS.gene09450:CDS | 15.0% |
| !! | GTAAAAAAAGATCTTGAAAA+TGG | + | chr6.4:23617021-23617040 | MS.gene09450:intron | 20.0% |
| ! | GAAAAAATATGGTAGCAATT+TGG | + | chr6.4:23616833-23616852 | MS.gene09450:CDS | 25.0% |
| ! | TAACAACAATTGAAGAAAAC+AGG | + | chr6.4:23617305-23617324 | MS.gene09450:CDS | 25.0% |
| ! | TATTCTATGAAGCATACTAA+TGG | - | chr6.4:23617407-23617426 | None:intergenic | 25.0% |
| !! | AAAAGCTTTATCGTAAAGAA+AGG | + | chr6.4:23616766-23616785 | MS.gene09450:CDS | 25.0% |
| !! | AGGATATTTGCTTTAGATAT+CGG | + | chr6.4:23617075-23617094 | MS.gene09450:intron | 25.0% |
| !! | TTGACGTGATTTTATCAAAA+AGG | + | chr6.4:23616913-23616932 | MS.gene09450:CDS | 25.0% |
| AAGAACAATCCTATTCTTGT+AGG | + | chr6.4:23616939-23616958 | MS.gene09450:CDS | 30.0% | |
| AATGAAATCCATCTTGTTCT+TGG | + | chr6.4:23617201-23617220 | MS.gene09450:CDS | 30.0% | |
| ATGAAATCCATCTTGTTCTT+GGG | + | chr6.4:23617202-23617221 | MS.gene09450:CDS | 30.0% | |
| GAAAATGGTAAAACAGATCT+TGG | + | chr6.4:23617036-23617055 | MS.gene09450:intron | 30.0% | |
| GGGAAAATAAACCCTATTAT+TGG | + | chr6.4:23616867-23616886 | MS.gene09450:CDS | 30.0% | |
| TAGAATAAAAGAGATGTACG+AGG | + | chr6.4:23617420-23617439 | MS.gene09450:CDS | 30.0% | |
| TGAAGCATACTAATGGTATT+TGG | - | chr6.4:23617400-23617419 | None:intergenic | 30.0% | |
| TGTCTTGAATTATAACGTCA+TGG | - | chr6.4:23617450-23617469 | None:intergenic | 30.0% | |
| TTGGAAATCTTGTTAATGCA+AGG | + | chr6.4:23617055-23617074 | MS.gene09450:intron | 30.0% | |
| ! | AGAAAAATTGCTGTTGTTGA+AGG | + | chr6.4:23616975-23616994 | MS.gene09450:CDS | 30.0% |
| ! | GAAAAATTGCTGTTGTTGAA+GGG | + | chr6.4:23616976-23616995 | MS.gene09450:CDS | 30.0% |
| ! | GTATGTTCTTTTGTACTCTA+AGG | + | chr6.4:23617515-23617534 | MS.gene09450:CDS | 30.0% |
| !!! | AAGAAAGTTGAAAGTGCTTT+TGG | + | chr6.4:23616789-23616808 | MS.gene09450:CDS | 30.0% |
| AAAAAGATGCAGCATTTGAG+AGG | + | chr6.4:23617338-23617357 | MS.gene09450:CDS | 35.0% | |
| AATTCTACCCAAGAACAAGA+TGG | - | chr6.4:23617212-23617231 | None:intergenic | 35.0% | |
| CTTGATCACGTCCAATAATA+GGG | - | chr6.4:23616881-23616900 | None:intergenic | 35.0% | |
| TCTTGATCACGTCCAATAAT+AGG | - | chr6.4:23616882-23616901 | None:intergenic | 35.0% | |
| TTGTCTAGTCAATACATAGC+TGG | + | chr6.4:23617493-23617512 | MS.gene09450:CDS | 35.0% | |
| TTGTTCTTGGGTAGAATTGA+AGG | + | chr6.4:23617214-23617233 | MS.gene09450:intron | 35.0% | |
| ! | ACAGCAATTTTTCTAACTCC+TGG | - | chr6.4:23616969-23616988 | None:intergenic | 35.0% |
| ! | CTAATGGTATTTGGAACACT+AGG | - | chr6.4:23617391-23617410 | None:intergenic | 35.0% |
| !! | AGATTTTGAAGAGAGGTTGA+AGG | + | chr6.4:23617128-23617147 | MS.gene09450:CDS | 35.0% |
| !! | ATGTACAAAAGCTTTGAAGC+TGG | + | chr6.4:23616711-23616730 | MS.gene09450:CDS | 35.0% |
| !! | GTTCTTTTGTACTCTAAGGA+CGG | + | chr6.4:23617519-23617538 | MS.gene09450:CDS | 35.0% |
| !!! | GAAGGAAGTTTTGAAAGAAG+TGG | + | chr6.4:23617146-23617165 | MS.gene09450:CDS | 35.0% |
| AAACCAATGCTTTATCGTGG+TGG | + | chr6.4:23617262-23617281 | MS.gene09450:intron | 40.0% | |
| AAAGAAGTGGAAGAAGCTGA+AGG | + | chr6.4:23617159-23617178 | MS.gene09450:CDS | 40.0% | |
| CTCAAACCAATGCTTTATCG+TGG | + | chr6.4:23617259-23617278 | MS.gene09450:intron | 40.0% | |
| GACGTGATCAAGAGATAGAA+AGG | + | chr6.4:23616889-23616908 | MS.gene09450:CDS | 40.0% | |
| GAGGTTTGTACAGATTGATG+TGG | + | chr6.4:23617360-23617379 | MS.gene09450:CDS | 40.0% | |
| GGGTAGAATTGAAGGATCAA+TGG | + | chr6.4:23617222-23617241 | MS.gene09450:intron | 40.0% | |
| GGTGGATATCTTAGATGCAT+TGG | + | chr6.4:23617280-23617299 | MS.gene09450:intron | 40.0% | |
| TATCCACCACGATAAAGCAT+TGG | - | chr6.4:23617268-23617287 | None:intergenic | 40.0% | |
| ! | AATTGCTGTTGTTGAAGGGT+TGG | + | chr6.4:23616980-23616999 | MS.gene09450:CDS | 40.0% |
| AAGATGCAGCATTTGAGAGG+AGG | + | chr6.4:23617341-23617360 | MS.gene09450:CDS | 45.0% | |
| AAGTGGAAGAAGCTGAAGGA+AGG | + | chr6.4:23617163-23617182 | MS.gene09450:CDS | 45.0% | |
| AGTGGAAGAAGCTGAAGGAA+GGG | + | chr6.4:23617164-23617183 | MS.gene09450:CDS | 45.0% | |
| TTGGCTCAAAGGATAGGTAG+AGG | + | chr6.4:23616999-23617018 | MS.gene09450:intron | 45.0% | |
| !! | AAGCTGGTGTCGATGTTTCT+AGG | + | chr6.4:23616727-23616746 | MS.gene09450:CDS | 45.0% |
| !! | AGCTGGTGTCGATGTTTCTA+GGG | + | chr6.4:23616728-23616747 | MS.gene09450:CDS | 45.0% |
| !! | TTGTTGAAGGGTTGGCTCAA+AGG | + | chr6.4:23616988-23617007 | MS.gene09450:CDS | 45.0% |
| AGCAATTTGGTTGAGCGAGC+AGG | + | chr6.4:23616846-23616865 | MS.gene09450:CDS | 50.0% | |
| CCTATTCTTGTAGGAGAGCC+AGG | + | chr6.4:23616948-23616967 | MS.gene09450:CDS | 50.0% | |
| GATATCGGCGCATTAGTTGC+TGG | + | chr6.4:23617090-23617109 | MS.gene09450:intron | 50.0% | |
| GCAATTTGGTTGAGCGAGCA+GGG | + | chr6.4:23616847-23616866 | MS.gene09450:CDS | 50.0% | |
| ! | AGTTGCTGGTGCTAAGAGCA+GGG | + | chr6.4:23617104-23617123 | MS.gene09450:CDS | 50.0% |
| ! | CCTGGCTCTCCTACAAGAAT+AGG | - | chr6.4:23616951-23616970 | None:intergenic | 50.0% |
| ! | GTACTCTAAGGACGGTTGTC+AGG | + | chr6.4:23617527-23617546 | MS.gene09450:CDS | 50.0% |
| ! | TAGTTGCTGGTGCTAAGAGC+AGG | + | chr6.4:23617103-23617122 | MS.gene09450:CDS | 50.0% |
| !! | GAAGGGTTGGCTCAAAGGAT+AGG | + | chr6.4:23616993-23617012 | MS.gene09450:CDS | 50.0% |
| !! | GCAGGGGAGATTTTGAAGAG+AGG | + | chr6.4:23617121-23617140 | MS.gene09450:CDS | 50.0% |
| ! | GTTGCTGGTGCTAAGAGCAG+GGG | + | chr6.4:23617105-23617124 | MS.gene09450:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 23616711 | 23617564 | 23616711 | ID=MS.gene09450 |
| chr6.4 | mRNA | 23616711 | 23617564 | 23616711 | ID=MS.gene09450.t1;Parent=MS.gene09450 |
| chr6.4 | exon | 23616711 | 23617014 | 23616711 | ID=MS.gene09450.t1.exon1;Parent=MS.gene09450.t1 |
| chr6.4 | CDS | 23616711 | 23617014 | 23616711 | ID=cds.MS.gene09450.t1;Parent=MS.gene09450.t1 |
| chr6.4 | exon | 23617091 | 23617223 | 23617091 | ID=MS.gene09450.t1.exon2;Parent=MS.gene09450.t1 |
| chr6.4 | CDS | 23617091 | 23617223 | 23617091 | ID=cds.MS.gene09450.t1;Parent=MS.gene09450.t1 |
| chr6.4 | exon | 23617294 | 23617564 | 23617294 | ID=MS.gene09450.t1.exon3;Parent=MS.gene09450.t1 |
| chr6.4 | CDS | 23617294 | 23617564 | 23617294 | ID=cds.MS.gene09450.t1;Parent=MS.gene09450.t1 |
| Gene Sequence |
| Protein sequence |
