Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09532.t1 | PNY15295.1 | 65.9 | 138 | 43 | 3 | 1 | 135 | 422 | 558 | 2.10E-37 | 165.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09532.t1 | A0A2K3PJ56 | 65.9 | 138 | 43 | 3 | 1 | 135 | 422 | 558 | 1.5e-37 | 165.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049452 | MS.gene09532 | 0.805477 | 1.31E-49 | -1.69E-46 |
| MS.gene05415 | MS.gene09532 | 0.81976 | 9.81E-53 | -1.69E-46 |
| MS.gene056272 | MS.gene09532 | 0.804426 | 2.17E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAGTGTGCGGAAGATTATC+TGG | 0.362382 | 1.2:-73979946 | MS.gene09532:CDS |
| TTCGGTTCTGTTTGCAACAT+TGG | 0.417204 | 1.2:+73979851 | None:intergenic |
| ACTCTGAAGATGATGAATAA+AGG | 0.431741 | 1.2:-73979620 | None:intergenic |
| TGGATCTTGAATAAATTGTT+CGG | 0.449685 | 1.2:+73979833 | None:intergenic |
| AAGCTTTAAATCAAAGATCA+AGG | 0.454195 | 1.2:-73979989 | MS.gene09532:CDS |
| CCAAAACGTGCTAAAACTAA+AGG | 0.463711 | 1.2:-73979813 | MS.gene09532:CDS |
| ACTAAAGGGCGTGGTGGTGC+AGG | 0.465724 | 1.2:-73979798 | MS.gene09532:CDS |
| ATCAAGGGATGTGAATGGTT+TGG | 0.468442 | 1.2:-73979973 | MS.gene09532:CDS |
| TGTGGTAAATGTGGAATTGT+TGG | 0.474301 | 1.2:-73979738 | MS.gene09532:CDS |
| GTCATGTATCTGATGAAGAT+TGG | 0.475848 | 1.2:-73979644 | MS.gene09532:CDS |
| CAAAACGTGCTAAAACTAAA+GGG | 0.507686 | 1.2:-73979812 | MS.gene09532:CDS |
| CACATTTACCACAATGTCCA+CGG | 0.523480 | 1.2:+73979748 | None:intergenic |
| CAAAGATCAAGGGATGTGAA+TGG | 0.572234 | 1.2:-73979978 | MS.gene09532:CDS |
| AAGAGGCGCCGTGGACATTG+TGG | 0.583174 | 1.2:-73979756 | MS.gene09532:CDS |
| GAAGCATGGCATACAATCTG+AGG | 0.592856 | 1.2:-73979880 | MS.gene09532:CDS |
| CGTGCTAAAACTAAAGGGCG+TGG | 0.593037 | 1.2:-73979807 | MS.gene09532:CDS |
| AGCTTTAAATCAAAGATCAA+GGG | 0.625411 | 1.2:-73979988 | MS.gene09532:CDS |
| CGTGGACATTGTGGTAAATG+TGG | 0.643084 | 1.2:-73979747 | MS.gene09532:CDS |
| GTGGTGCAGGAACTTCTAGA+CGG | 0.688641 | 1.2:-73979785 | MS.gene09532:CDS |
| GCTAAAACTAAAGGGCGTGG+TGG | 0.708813 | 1.2:-73979804 | MS.gene09532:CDS |
| TGGTTTGGCATGCAAGTGTG+CGG | 0.709047 | 1.2:-73979958 | MS.gene09532:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAACATTGAAAAAAGTAAA+TGG | - | chr1.2:73979935-73979954 | MS.gene09532:CDS | 20.0% |
| ! | TGGATCTTGAATAAATTGTT+CGG | + | chr1.2:73979801-73979820 | None:intergenic | 25.0% |
| !!! | AAGCTTTAAATCAAAGATCA+AGG | - | chr1.2:73979642-73979661 | MS.gene09532:CDS | 25.0% |
| !!! | AGCTTTAAATCAAAGATCAA+GGG | - | chr1.2:73979643-73979662 | MS.gene09532:CDS | 25.0% |
| !!! | TCTTTTTAAGACTCTCATAA+TGG | + | chr1.2:73979733-73979752 | None:intergenic | 25.0% |
| CAAAACGTGCTAAAACTAAA+GGG | - | chr1.2:73979819-73979838 | MS.gene09532:CDS | 30.0% | |
| CCAAAACGTGCTAAAACTAA+AGG | - | chr1.2:73979818-73979837 | MS.gene09532:CDS | 35.0% | |
| CTTCTAGACGGAAAAATAAG+AGG | - | chr1.2:73979858-73979877 | MS.gene09532:CDS | 35.0% | |
| GAGAGTCTTAAAAAGAAGCA+TGG | - | chr1.2:73979737-73979756 | MS.gene09532:CDS | 35.0% | |
| GTCATGTATCTGATGAAGAT+TGG | - | chr1.2:73979987-73980006 | MS.gene09532:CDS | 35.0% | |
| TGTGGTAAATGTGGAATTGT+TGG | - | chr1.2:73979893-73979912 | MS.gene09532:CDS | 35.0% | |
| !!! | CCTTTAGTTTTAGCACGTTT+TGG | + | chr1.2:73979821-73979840 | None:intergenic | 35.0% |
| ATCAAGGGATGTGAATGGTT+TGG | - | chr1.2:73979658-73979677 | MS.gene09532:CDS | 40.0% | |
| CAAAGATCAAGGGATGTGAA+TGG | - | chr1.2:73979653-73979672 | MS.gene09532:CDS | 40.0% | |
| CACATTTACCACAATGTCCA+CGG | + | chr1.2:73979886-73979905 | None:intergenic | 40.0% | |
| TTCGGTTCTGTTTGCAACAT+TGG | + | chr1.2:73979783-73979802 | None:intergenic | 40.0% | |
| CAAGTGTGCGGAAGATTATC+TGG | - | chr1.2:73979685-73979704 | MS.gene09532:CDS | 45.0% | |
| CGTGGACATTGTGGTAAATG+TGG | - | chr1.2:73979884-73979903 | MS.gene09532:CDS | 45.0% | |
| GAAGCATGGCATACAATCTG+AGG | - | chr1.2:73979751-73979770 | MS.gene09532:CDS | 45.0% | |
| CGTGCTAAAACTAAAGGGCG+TGG | - | chr1.2:73979824-73979843 | MS.gene09532:CDS | 50.0% | |
| GTGGTGCAGGAACTTCTAGA+CGG | - | chr1.2:73979846-73979865 | MS.gene09532:CDS | 50.0% | |
| ! | GCTAAAACTAAAGGGCGTGG+TGG | - | chr1.2:73979827-73979846 | MS.gene09532:CDS | 50.0% |
| ! | TGGTTTGGCATGCAAGTGTG+CGG | - | chr1.2:73979673-73979692 | MS.gene09532:CDS | 50.0% |
| !! | CGGAAAAATAAGAGGCGCCG+TGG | - | chr1.2:73979866-73979885 | MS.gene09532:CDS | 55.0% |
| ! | ACTAAAGGGCGTGGTGGTGC+AGG | - | chr1.2:73979833-73979852 | MS.gene09532:CDS | 60.0% |
| !! | AAGAGGCGCCGTGGACATTG+TGG | - | chr1.2:73979875-73979894 | MS.gene09532:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 73979623 | 73980030 | 73979623 | ID=MS.gene09532 |
| chr1.2 | mRNA | 73979623 | 73980030 | 73979623 | ID=MS.gene09532.t1;Parent=MS.gene09532 |
| chr1.2 | exon | 73979623 | 73980030 | 73979623 | ID=MS.gene09532.t1.exon1;Parent=MS.gene09532.t1 |
| chr1.2 | CDS | 73979623 | 73980030 | 73979623 | ID=cds.MS.gene09532.t1;Parent=MS.gene09532.t1 |
| Gene Sequence |
| Protein sequence |
