AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene09538


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09538.t1 MTR_5g016540 98.901 182 2 0 1 182 1 182 1.79e-134 373
MS.gene09538.t1 MTR_5g034130 87.778 180 22 0 1 180 1 180 9.34e-121 338
MS.gene09538.t1 MTR_5g034125 87.778 180 22 0 1 180 1 180 9.34e-121 338
MS.gene09538.t1 MTR_1g086480 87.222 180 23 0 1 180 1 180 3.06e-120 337
MS.gene09538.t1 MTR_6g005820 87.222 180 23 0 1 180 1 180 5.17e-120 337
MS.gene09538.t1 MTR_7g109960 86.667 180 24 0 1 180 1 180 1.52e-119 335
MS.gene09538.t1 MTR_5g007810 59.887 177 71 0 1 177 1 177 2.56e-80 237
MS.gene09538.t1 MTR_4g077640 58.757 177 73 0 1 177 1 177 1.90e-77 229
MS.gene09538.t1 MTR_4g128910 56.983 179 77 0 1 179 1 179 6.89e-77 228
MS.gene09538.t1 MTR_5g007820 53.846 182 82 1 1 180 1 182 1.04e-72 217
MS.gene09538.t1 MTR_1g076550 50.556 180 89 0 1 180 1 180 8.51e-66 199
MS.gene09538.t1 MTR_5g007840 41.315 213 80 2 1 180 1 201 1.71e-54 172
MS.gene09538.t1 MTR_8g077540 47.590 166 84 3 18 181 17 181 4.14e-49 157
MS.gene09538.t1 MTR_8g077540 47.590 166 84 3 18 181 43 207 5.82e-49 158
MS.gene09538.t1 MTR_2g022200 34.682 173 102 2 10 173 10 180 5.06e-36 124
MS.gene09538.t1 MTR_5g078090 34.337 166 108 1 9 173 11 176 1.97e-30 110
MS.gene09538.t1 MTR_5g015370 33.735 166 109 1 9 173 11 176 1.61e-29 107
MS.gene09538.t1 MTR_8g077540 51.020 98 46 2 18 114 17 113 7.67e-29 104
MS.gene09538.t1 MTR_4g026950 33.121 157 104 1 18 173 1 157 2.81e-26 98.6
MS.gene09538.t1 MTR_2g034640 32.768 177 103 2 15 177 18 192 1.16e-25 97.8
MS.gene09538.t1 MTR_7g112090 32.203 177 104 2 15 177 18 192 2.61e-25 97.1
MS.gene09538.t1 MTR_4g121860 32.203 177 104 2 15 177 18 192 9.13e-24 92.8
MS.gene09538.t1 MTR_1g062760 35.294 85 51 1 18 98 15 99 2.10e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09538.t1 AT3G62290 87.222 180 23 0 1 180 1 180 5.98e-120 337
MS.gene09538.t1 AT3G62290 87.222 180 23 0 1 180 1 180 5.98e-120 337
MS.gene09538.t1 AT3G62290 87.222 180 23 0 1 180 1 180 5.98e-120 337
MS.gene09538.t1 AT1G10630 86.667 180 24 0 1 180 1 180 1.68e-119 335
MS.gene09538.t1 AT1G10630 86.667 180 24 0 1 180 1 180 1.68e-119 335
MS.gene09538.t1 AT1G70490 86.667 180 24 0 1 180 1 180 2.60e-119 335
MS.gene09538.t1 AT1G70490 86.667 180 24 0 1 180 1 180 2.60e-119 335
MS.gene09538.t1 AT1G70490 86.667 180 24 0 1 180 1 180 2.60e-119 335
MS.gene09538.t1 AT1G70490 86.667 180 24 0 1 180 1 180 2.60e-119 335
MS.gene09538.t1 AT1G70490 86.667 180 24 0 1 180 1 180 2.60e-119 335
MS.gene09538.t1 AT1G70490 86.667 180 24 0 1 180 1 180 2.60e-119 335
MS.gene09538.t1 AT1G23490 86.667 180 24 0 1 180 1 180 2.60e-119 335
MS.gene09538.t1 AT1G70490 86.667 180 24 0 1 180 1 180 2.60e-119 335
MS.gene09538.t1 AT5G14670 86.188 181 25 0 1 181 1 181 2.67e-119 335
MS.gene09538.t1 AT2G47170 86.667 180 24 0 1 180 1 180 3.82e-119 335
MS.gene09538.t1 AT2G47170 86.667 180 24 0 1 180 1 180 3.82e-119 335
MS.gene09538.t1 AT2G47170 86.667 180 24 0 1 180 1 180 3.82e-119 335
MS.gene09538.t1 AT5G14670 86.667 180 24 0 1 180 1 180 4.65e-119 334
MS.gene09538.t1 AT5G14670 86.667 180 24 0 1 180 1 180 4.65e-119 334
MS.gene09538.t1 AT2G15310 70.000 180 54 0 1 180 1 180 1.91e-93 270
MS.gene09538.t1 AT5G17060 58.659 179 74 0 1 179 1 179 7.02e-80 236
MS.gene09538.t1 AT5G17060 58.659 179 74 0 1 179 1 179 7.02e-80 236
MS.gene09538.t1 AT3G03120 59.322 177 72 0 1 177 1 177 3.08e-79 234
MS.gene09538.t1 AT2G24765 57.542 179 76 0 1 179 1 179 3.26e-78 231
MS.gene09538.t1 AT3G22950 51.667 180 87 0 1 180 1 180 4.47e-69 208
MS.gene09538.t1 AT3G22950 51.667 180 87 0 1 180 1 180 4.47e-69 208
MS.gene09538.t1 AT3G03120 61.538 130 50 0 1 130 1 130 3.62e-58 179
MS.gene09538.t1 AT2G24765 60.156 128 51 0 1 128 1 128 1.54e-55 172
MS.gene09538.t1 AT1G02430 46.774 186 89 4 1 176 1 186 6.34e-54 170
MS.gene09538.t1 AT1G02440 44.624 186 93 4 1 176 1 186 2.19e-53 168
MS.gene09538.t1 AT2G18390 47.590 166 84 3 18 181 17 181 2.88e-48 155
MS.gene09538.t1 AT1G02430 44.654 159 82 3 1 153 1 159 1.96e-42 140
MS.gene09538.t1 AT5G52210 32.955 176 111 1 5 173 5 180 3.13e-34 120
MS.gene09538.t1 AT5G52210 32.955 176 111 1 5 173 5 180 3.13e-34 120
MS.gene09538.t1 AT3G49870 34.940 166 107 1 9 173 11 176 3.70e-32 114
MS.gene09538.t1 AT5G67560 34.337 166 108 1 9 173 11 176 1.04e-31 113
MS.gene09538.t1 AT3G49870 35.032 157 101 1 18 173 1 157 7.85e-30 107
MS.gene09538.t1 AT5G37680 33.133 166 110 1 9 173 11 176 8.27e-30 108
MS.gene09538.t1 AT3G49860 34.940 166 107 1 16 180 10 175 2.24e-29 107
MS.gene09538.t1 AT3G62560 32.768 177 103 2 15 177 18 192 2.01e-25 97.4
MS.gene09538.t1 AT4G02080 32.000 175 107 2 15 177 18 192 5.84e-25 96.3
MS.gene09538.t1 AT1G56330 32.584 178 104 2 15 178 18 193 7.39e-25 95.9
MS.gene09538.t1 AT1G02440 31.818 154 69 2 1 153 1 119 1.33e-24 93.2
MS.gene09538.t1 AT1G09180 32.584 178 104 2 14 177 17 192 1.59e-24 95.1
MS.gene09538.t1 AT5G37680 33.594 128 85 0 46 173 2 129 5.11e-24 92.0

Find 33 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTGGAAGGTCTTGCTTAT+TGG 0.247726 5.2:-8249036 None:intergenic
AAAATGTCAGCTTCACTGTC+TGG 0.376569 5.2:+8248490 MS.gene09538:CDS
TCTGCAATGCTCAAAGCATT+TGG 0.384037 5.2:-8249053 None:intergenic
AGCTTCACTGTCTGGGATGT+TGG 0.414580 5.2:+8248498 MS.gene09538:CDS
GACTCTATGAAGGCCTTGAT+TGG 0.421761 5.2:+8249276 MS.gene09538:CDS
CAGTCAACTTGTGCCACGTC+TGG 0.434429 5.2:+8249248 MS.gene09538:CDS
TACACAAGGTCTTATCTTTG+TGG 0.464373 5.2:+8248808 MS.gene09538:CDS
CAGACGTGGCACAAGTTGAC+TGG 0.490705 5.2:-8249247 None:intergenic
CATTCTATGAAGTTCATCTC+TGG 0.495248 5.2:-8248864 None:intergenic
TTTGGAACCTATGGTTGGTA+TGG 0.503874 5.2:-8248334 None:intergenic
TTTATGCAAAGAAAGAAATG+AGG 0.508896 5.2:+8248235 MS.gene09538:CDS
GCAGAAATTACTGATAAACT+TGG 0.510496 5.2:+8249071 MS.gene09538:CDS
ATTCACTTCGCCAGCGTCGT+TGG 0.511745 5.2:+8249099 MS.gene09538:CDS
ATACTCTACAAACTGAAGCT+TGG 0.516539 5.2:+8248297 MS.gene09538:CDS
AAATGTCAGCTTCACTGTCT+GGG 0.518950 5.2:+8248491 MS.gene09538:CDS
ATGGTAGGTCTTGATGCTGC+TGG 0.522575 5.2:+8248264 MS.gene09538:CDS
TTCACTGTCTGGGATGTTGG+AGG 0.531178 5.2:+8248501 MS.gene09538:CDS
TGTTACTTGATAACCAATCA+AGG 0.534396 5.2:-8249289 None:intergenic
ATTGGCAAATACAAGTAAAG+TGG 0.538360 5.2:-8249018 None:intergenic
GACTATTTGGAACCTATGGT+TGG 0.543143 5.2:-8248339 None:intergenic
GAAAGAAATGAGGATTCTGA+TGG 0.583316 5.2:+8248245 MS.gene09538:CDS
GAAATGAGGATTCTGATGGT+AGG 0.584694 5.2:+8248249 MS.gene09538:CDS
ACTTGTGCCACGTCTGGACA+AGG 0.593035 5.2:+8249254 MS.gene09538:CDS
TCGTGTAGATTCGACCATTG+TGG 0.593428 5.2:+8248768 MS.gene09538:intron
CAATGCTCAAAGCATTTGGA+AGG 0.595533 5.2:-8249049 None:intergenic
TCTGGACAAGGACTCTATGA+AGG 0.595755 5.2:+8249266 MS.gene09538:CDS
ACGTGACTTACCAACGACGC+TGG 0.609941 5.2:-8249109 None:intergenic
TGAAAATAGTGTCTCCACAA+TGG 0.619967 5.2:-8248782 None:intergenic
ATAGAGTCCTTGTCCAGACG+TGG 0.637305 5.2:-8249261 None:intergenic
GGATGTTGGAGGACAAGACA+AGG 0.648549 5.2:+8248512 MS.gene09538:CDS
AAATGGGGTTGACAGTGTCA+CGG 0.652051 5.2:+8248199 None:intergenic
GTCACTACCATACCAACCAT+AGG 0.654409 5.2:+8248327 MS.gene09538:CDS
ACTTCATAGAATGCTTAGTG+AGG 0.676324 5.2:+8248874 MS.gene09538:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATAGATATTATTTTTCAA+TGG - chr5.2:8248364-8248383 None:intergenic 10.0%
!! TTAATAAGCATTTACATTGT+AGG + chr5.2:8248980-8248999 MS.gene09538:intron 20.0%
!!! TATTTCACTTTTATGTTGAA+AGG - chr5.2:8248946-8248965 None:intergenic 20.0%
!!! TTATTTTTCAATGGACTATT+TGG - chr5.2:8248355-8248374 None:intergenic 20.0%
! TTAAAATGATCATAGGGAAT+AGG - chr5.2:8248394-8248413 None:intergenic 25.0%
! TTTAATGTCTCAAATGAATC+AGG + chr5.2:8248441-8248460 MS.gene09538:intron 25.0%
! TTTATGCAAAGAAAGAAATG+AGG + chr5.2:8248235-8248254 MS.gene09538:CDS 25.0%
! TTTCGCTTAAAATGATCATA+GGG - chr5.2:8248400-8248419 None:intergenic 25.0%
!! ACTTGTTAGAACATATAAGA+AGG - chr5.2:8248696-8248715 None:intergenic 25.0%
!! TTTTCGCTTAAAATGATCAT+AGG - chr5.2:8248401-8248420 None:intergenic 25.0%
AAGCTTCAGATAATGATTCA+TGG - chr5.2:8249143-8249162 None:intergenic 30.0%
ATAATCCAAAGTTGAGTTCA+GGG + chr5.2:8249200-8249219 MS.gene09538:intron 30.0%
ATTGGCAAATACAAGTAAAG+TGG - chr5.2:8249021-8249040 None:intergenic 30.0%
CATAGTTCAATCAAGTGAAA+GGG - chr5.2:8248657-8248676 None:intergenic 30.0%
GCAGAAATTACTGATAAACT+TGG + chr5.2:8249071-8249090 MS.gene09538:CDS 30.0%
GTTAGAACATATAAGAAGGT+TGG - chr5.2:8248692-8248711 None:intergenic 30.0%
TCATAGTTCAATCAAGTGAA+AGG - chr5.2:8248658-8248677 None:intergenic 30.0%
! CACTATTTTCAGAATACACA+AGG + chr5.2:8248794-8248813 MS.gene09538:CDS 30.0%
! TCTAGTTTGCAATATGAACA+TGG - chr5.2:8248570-8248589 None:intergenic 30.0%
! TGTTACTTGATAACCAATCA+AGG - chr5.2:8249292-8249311 None:intergenic 30.0%
ACTTCATAGAATGCTTAGTG+AGG + chr5.2:8248874-8248893 MS.gene09538:CDS 35.0%
AGGTAATTCAGTTTGCACAT+TGG + chr5.2:8248624-8248643 MS.gene09538:intron 35.0%
ATACTCTACAAACTGAAGCT+TGG + chr5.2:8248297-8248316 MS.gene09538:CDS 35.0%
ATTTGGAAGGTCTTGCTTAT+TGG - chr5.2:8249039-8249058 None:intergenic 35.0%
CATAATCCAAAGTTGAGTTC+AGG + chr5.2:8249199-8249218 MS.gene09538:intron 35.0%
CATTCTATGAAGTTCATCTC+TGG - chr5.2:8248867-8248886 None:intergenic 35.0%
GAAAGAAATGAGGATTCTGA+TGG + chr5.2:8248245-8248264 MS.gene09538:CDS 35.0%
TGAAAATAGTGTCTCCACAA+TGG - chr5.2:8248785-8248804 None:intergenic 35.0%
! TACACAAGGTCTTATCTTTG+TGG + chr5.2:8248808-8248827 MS.gene09538:CDS 35.0%
!! AATGGACTATTTGGAACCTA+TGG - chr5.2:8248346-8248365 None:intergenic 35.0%
AAAATGTCAGCTTCACTGTC+TGG + chr5.2:8248490-8248509 MS.gene09538:CDS 40.0%
AAACACCCTGAACTCAACTT+TGG - chr5.2:8249208-8249227 None:intergenic 40.0%
AAATGTCAGCTTCACTGTCT+GGG + chr5.2:8248491-8248510 MS.gene09538:CDS 40.0%
GAAATGAGGATTCTGATGGT+AGG + chr5.2:8248249-8248268 MS.gene09538:CDS 40.0%
! CAATGCTCAAAGCATTTGGA+AGG - chr5.2:8249052-8249071 None:intergenic 40.0%
! GACTATTTGGAACCTATGGT+TGG - chr5.2:8248342-8248361 None:intergenic 40.0%
! TCTGCAATGCTCAAAGCATT+TGG - chr5.2:8249056-8249075 None:intergenic 40.0%
! TTTGGAACCTATGGTTGGTA+TGG - chr5.2:8248337-8248356 None:intergenic 40.0%
GACTCTATGAAGGCCTTGAT+TGG + chr5.2:8249276-8249295 MS.gene09538:CDS 45.0%
GTCACTACCATACCAACCAT+AGG + chr5.2:8248327-8248346 MS.gene09538:CDS 45.0%
GTTGACTGGATGTACCTGAA+CGG - chr5.2:8249236-8249255 None:intergenic 45.0%
TCGTGTAGATTCGACCATTG+TGG + chr5.2:8248768-8248787 MS.gene09538:intron 45.0%
TCTGGACAAGGACTCTATGA+AGG + chr5.2:8249266-8249285 MS.gene09538:CDS 45.0%
!! AGGGTGTTTTTGATCCGTTC+AGG + chr5.2:8249219-8249238 MS.gene09538:intron 45.0%
!! TAATAATAATAATAATAATG+AGG + chr5.2:8248604-8248623 MS.gene09538:intron 5.0%
ATAGAGTCCTTGTCCAGACG+TGG - chr5.2:8249264-8249283 None:intergenic 50.0%
ATGGTAGGTCTTGATGCTGC+TGG + chr5.2:8248264-8248283 MS.gene09538:CDS 50.0%
GGATGTTGGAGGACAAGACA+AGG + chr5.2:8248512-8248531 MS.gene09538:CDS 50.0%
! AGCTTCACTGTCTGGGATGT+TGG + chr5.2:8248498-8248517 MS.gene09538:CDS 50.0%
! TTCACTGTCTGGGATGTTGG+AGG + chr5.2:8248501-8248520 MS.gene09538:CDS 50.0%
ACGTGACTTACCAACGACGC+TGG - chr5.2:8249112-8249131 None:intergenic 55.0%
ACTTGTGCCACGTCTGGACA+AGG + chr5.2:8249254-8249273 MS.gene09538:CDS 55.0%
ATTCACTTCGCCAGCGTCGT+TGG + chr5.2:8249099-8249118 MS.gene09538:CDS 55.0%
CAGTCAACTTGTGCCACGTC+TGG + chr5.2:8249248-8249267 MS.gene09538:CDS 55.0%
! CAGACGTGGCACAAGTTGAC+TGG - chr5.2:8249250-8249269 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 8248201 8249331 8248201 ID=MS.gene09538
chr5.2 mRNA 8248201 8249331 8248201 ID=MS.gene09538.t1;Parent=MS.gene09538
chr5.2 exon 8248201 8248348 8248201 ID=MS.gene09538.t1.exon1;Parent=MS.gene09538.t1
chr5.2 CDS 8248201 8248348 8248201 ID=cds.MS.gene09538.t1;Parent=MS.gene09538.t1
chr5.2 exon 8248463 8248533 8248463 ID=MS.gene09538.t1.exon2;Parent=MS.gene09538.t1
chr5.2 CDS 8248463 8248533 8248463 ID=cds.MS.gene09538.t1;Parent=MS.gene09538.t1
chr5.2 exon 8248776 8248895 8248776 ID=MS.gene09538.t1.exon3;Parent=MS.gene09538.t1
chr5.2 CDS 8248776 8248895 8248776 ID=cds.MS.gene09538.t1;Parent=MS.gene09538.t1
chr5.2 exon 8249002 8249120 8249002 ID=MS.gene09538.t1.exon4;Parent=MS.gene09538.t1
chr5.2 CDS 8249002 8249120 8249002 ID=cds.MS.gene09538.t1;Parent=MS.gene09538.t1
chr5.2 exon 8249241 8249331 8249241 ID=MS.gene09538.t1.exon5;Parent=MS.gene09538.t1
chr5.2 CDS 8249241 8249331 8249241 ID=cds.MS.gene09538.t1;Parent=MS.gene09538.t1
Gene Sequence

>MS.gene09538

ATGGGGTTGACAGTGTCACGGATAATGAGATTATTTTATGCAAAGAAAGAAATGAGGATTCTGATGGTAGGTCTTGATGCTGCTGGAAAAACAACAATACTCTACAAACTGAAGCTTGGAGAAATTGTCACTACCATACCAACCATAGGTTCCAAATAGTCCATTGAAAAATAATATCTATTTATTTTTTCCTATTCCCTATGATCATTTTAAGCGAAAAATGATTGAAAATTGACATAATTTAATGTCTCAAATGAATCAGGCTTCAATGTAGAGACTGTTGAGTACAAAAATGTCAGCTTCACTGTCTGGGATGTTGGAGGACAAGACAAGGTATCATTCTTGAAATGCATTCTTTTCTAGCTTCCATGTTCATATTGCAAACTAGAAATGCATGCTTGTTTAATAATAATAATAATAATGAGGTAATTCAGTTTGCACATTGGTAATACACCCTTTCACTTGATTGAACTATGAACTAATACTAACCAACCTTCTTATATGTTCTAACAAGTATTTCTTGTATGATTCACATGTTACTCTTCTGATATGAGTATATGCTAAATTTCGTGTAGATTCGACCATTGTGGAGACACTATTTTCAGAATACACAAGGTCTTATCTTTGTGGTAGATAGTAATGATAGAGAAAGAATTTTAGAAGCCAGAGATGAACTTCATAGAATGCTTAGTGAGGTTAGTAGCTTTAAAATAGTCTAGTTTTTTAAGTATTACAAAACTTTCCTTTCAACATAAAAGTGAAATATTTTTGCAATCATATTAATAAGCATTTACATTGTAGGATGAACTTCGTGATGCCACTTTACTTGTATTTGCCAATAAGCAAGACCTTCCAAATGCTTTGAGCATTGCAGAAATTACTGATAAACTTGGTTTACATTCACTTCGCCAGCGTCGTTGGTAAGTCACGTTTTACTTACCATGAATCATTATCTGAAGCTTGAAATCATAGATTGAAACTTCAAATGTGAATTACATCATAATCCAAAGTTGAGTTCAGGGTGTTTTTGATCCGTTCAGGTACATCCAGTCAACTTGTGCCACGTCTGGACAAGGACTCTATGAAGGCCTTGATTGGTTATCAAGTAACATATCTAACAAGATAAGATGA

Protein sequence

>MS.gene09538.t1

MGLTVSRIMRLFYAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNVSFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERILEARDELHRMLSEDELRDATLLVFANKQDLPNALSIAEITDKLGLHSLRQRRWYIQSTCATSGQGLYEGLDWLSSNISNKIR