AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene09541


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09541.t1 MTR_5g016540 98.901 182 2 0 1 182 1 182 1.79e-134 373
MS.gene09541.t1 MTR_5g034130 87.778 180 22 0 1 180 1 180 9.34e-121 338
MS.gene09541.t1 MTR_5g034125 87.778 180 22 0 1 180 1 180 9.34e-121 338
MS.gene09541.t1 MTR_1g086480 87.222 180 23 0 1 180 1 180 3.06e-120 337
MS.gene09541.t1 MTR_6g005820 87.222 180 23 0 1 180 1 180 5.17e-120 337
MS.gene09541.t1 MTR_7g109960 86.667 180 24 0 1 180 1 180 1.52e-119 335
MS.gene09541.t1 MTR_5g007810 59.887 177 71 0 1 177 1 177 2.56e-80 237
MS.gene09541.t1 MTR_4g077640 58.757 177 73 0 1 177 1 177 1.90e-77 229
MS.gene09541.t1 MTR_4g128910 56.983 179 77 0 1 179 1 179 6.89e-77 228
MS.gene09541.t1 MTR_5g007820 53.846 182 82 1 1 180 1 182 1.04e-72 217
MS.gene09541.t1 MTR_1g076550 50.556 180 89 0 1 180 1 180 8.51e-66 199
MS.gene09541.t1 MTR_5g007840 41.315 213 80 2 1 180 1 201 1.71e-54 172
MS.gene09541.t1 MTR_8g077540 47.590 166 84 3 18 181 17 181 4.14e-49 157
MS.gene09541.t1 MTR_8g077540 47.590 166 84 3 18 181 43 207 5.82e-49 158
MS.gene09541.t1 MTR_2g022200 34.682 173 102 2 10 173 10 180 5.06e-36 124
MS.gene09541.t1 MTR_5g078090 34.337 166 108 1 9 173 11 176 1.97e-30 110
MS.gene09541.t1 MTR_5g015370 33.735 166 109 1 9 173 11 176 1.61e-29 107
MS.gene09541.t1 MTR_8g077540 51.020 98 46 2 18 114 17 113 7.67e-29 104
MS.gene09541.t1 MTR_4g026950 33.121 157 104 1 18 173 1 157 2.81e-26 98.6
MS.gene09541.t1 MTR_2g034640 32.768 177 103 2 15 177 18 192 1.16e-25 97.8
MS.gene09541.t1 MTR_7g112090 32.203 177 104 2 15 177 18 192 2.61e-25 97.1
MS.gene09541.t1 MTR_4g121860 32.203 177 104 2 15 177 18 192 9.13e-24 92.8
MS.gene09541.t1 MTR_1g062760 35.294 85 51 1 18 98 15 99 2.10e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09541.t1 AT3G62290 87.222 180 23 0 1 180 1 180 5.98e-120 337
MS.gene09541.t1 AT3G62290 87.222 180 23 0 1 180 1 180 5.98e-120 337
MS.gene09541.t1 AT3G62290 87.222 180 23 0 1 180 1 180 5.98e-120 337
MS.gene09541.t1 AT1G10630 86.667 180 24 0 1 180 1 180 1.68e-119 335
MS.gene09541.t1 AT1G10630 86.667 180 24 0 1 180 1 180 1.68e-119 335
MS.gene09541.t1 AT1G70490 86.667 180 24 0 1 180 1 180 2.60e-119 335
MS.gene09541.t1 AT1G70490 86.667 180 24 0 1 180 1 180 2.60e-119 335
MS.gene09541.t1 AT1G70490 86.667 180 24 0 1 180 1 180 2.60e-119 335
MS.gene09541.t1 AT1G70490 86.667 180 24 0 1 180 1 180 2.60e-119 335
MS.gene09541.t1 AT1G70490 86.667 180 24 0 1 180 1 180 2.60e-119 335
MS.gene09541.t1 AT1G70490 86.667 180 24 0 1 180 1 180 2.60e-119 335
MS.gene09541.t1 AT1G23490 86.667 180 24 0 1 180 1 180 2.60e-119 335
MS.gene09541.t1 AT1G70490 86.667 180 24 0 1 180 1 180 2.60e-119 335
MS.gene09541.t1 AT5G14670 86.188 181 25 0 1 181 1 181 2.67e-119 335
MS.gene09541.t1 AT2G47170 86.667 180 24 0 1 180 1 180 3.82e-119 335
MS.gene09541.t1 AT2G47170 86.667 180 24 0 1 180 1 180 3.82e-119 335
MS.gene09541.t1 AT2G47170 86.667 180 24 0 1 180 1 180 3.82e-119 335
MS.gene09541.t1 AT5G14670 86.667 180 24 0 1 180 1 180 4.65e-119 334
MS.gene09541.t1 AT5G14670 86.667 180 24 0 1 180 1 180 4.65e-119 334
MS.gene09541.t1 AT2G15310 70.000 180 54 0 1 180 1 180 1.91e-93 270
MS.gene09541.t1 AT5G17060 58.659 179 74 0 1 179 1 179 7.02e-80 236
MS.gene09541.t1 AT5G17060 58.659 179 74 0 1 179 1 179 7.02e-80 236
MS.gene09541.t1 AT3G03120 59.322 177 72 0 1 177 1 177 3.08e-79 234
MS.gene09541.t1 AT2G24765 57.542 179 76 0 1 179 1 179 3.26e-78 231
MS.gene09541.t1 AT3G22950 51.667 180 87 0 1 180 1 180 4.47e-69 208
MS.gene09541.t1 AT3G22950 51.667 180 87 0 1 180 1 180 4.47e-69 208
MS.gene09541.t1 AT3G03120 61.538 130 50 0 1 130 1 130 3.62e-58 179
MS.gene09541.t1 AT2G24765 60.156 128 51 0 1 128 1 128 1.54e-55 172
MS.gene09541.t1 AT1G02430 46.774 186 89 4 1 176 1 186 6.34e-54 170
MS.gene09541.t1 AT1G02440 44.624 186 93 4 1 176 1 186 2.19e-53 168
MS.gene09541.t1 AT2G18390 47.590 166 84 3 18 181 17 181 2.88e-48 155
MS.gene09541.t1 AT1G02430 44.654 159 82 3 1 153 1 159 1.96e-42 140
MS.gene09541.t1 AT5G52210 32.955 176 111 1 5 173 5 180 3.13e-34 120
MS.gene09541.t1 AT5G52210 32.955 176 111 1 5 173 5 180 3.13e-34 120
MS.gene09541.t1 AT3G49870 34.940 166 107 1 9 173 11 176 3.70e-32 114
MS.gene09541.t1 AT5G67560 34.337 166 108 1 9 173 11 176 1.04e-31 113
MS.gene09541.t1 AT3G49870 35.032 157 101 1 18 173 1 157 7.85e-30 107
MS.gene09541.t1 AT5G37680 33.133 166 110 1 9 173 11 176 8.27e-30 108
MS.gene09541.t1 AT3G49860 34.940 166 107 1 16 180 10 175 2.24e-29 107
MS.gene09541.t1 AT3G62560 32.768 177 103 2 15 177 18 192 2.01e-25 97.4
MS.gene09541.t1 AT4G02080 32.000 175 107 2 15 177 18 192 5.84e-25 96.3
MS.gene09541.t1 AT1G56330 32.584 178 104 2 15 178 18 193 7.39e-25 95.9
MS.gene09541.t1 AT1G02440 31.818 154 69 2 1 153 1 119 1.33e-24 93.2
MS.gene09541.t1 AT1G09180 32.584 178 104 2 14 177 17 192 1.59e-24 95.1
MS.gene09541.t1 AT5G37680 33.594 128 85 0 46 173 2 129 5.11e-24 92.0

Find 33 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTGGAAGGTCTTGCTTAT+TGG 0.247726 5.2:-8218507 None:intergenic
AAAATGTCAGCTTCACTGTC+TGG 0.376569 5.2:+8217961 MS.gene09541:CDS
TCTGCAATGCTCAAAGCATT+TGG 0.384037 5.2:-8218524 None:intergenic
AGCTTCACTGTCTGGGATGT+TGG 0.414580 5.2:+8217969 MS.gene09541:CDS
GACTCTATGAAGGCCTTGAT+TGG 0.421761 5.2:+8218747 MS.gene09541:CDS
CAGTCAACTTGTGCCACGTC+TGG 0.434429 5.2:+8218719 MS.gene09541:CDS
TACACAAGGTCTTATCTTTG+TGG 0.464373 5.2:+8218279 MS.gene09541:CDS
CAGACGTGGCACAAGTTGAC+TGG 0.490705 5.2:-8218718 None:intergenic
CATTCTATGAAGTTCATCTC+TGG 0.495248 5.2:-8218335 None:intergenic
TTTGGAACCTATGGTTGGTA+TGG 0.503874 5.2:-8217805 None:intergenic
TTTATGCAAAGAAAGAAATG+AGG 0.508896 5.2:+8217706 MS.gene09541:CDS
GCAGAAATTACTGATAAACT+TGG 0.510496 5.2:+8218542 MS.gene09541:CDS
ATTCACTTCGCCAGCGTCGT+TGG 0.511745 5.2:+8218570 MS.gene09541:CDS
ATACTCTACAAACTGAAGCT+TGG 0.516539 5.2:+8217768 MS.gene09541:CDS
AAATGTCAGCTTCACTGTCT+GGG 0.518950 5.2:+8217962 MS.gene09541:CDS
ATGGTAGGTCTTGATGCTGC+TGG 0.522575 5.2:+8217735 MS.gene09541:CDS
TTCACTGTCTGGGATGTTGG+AGG 0.531178 5.2:+8217972 MS.gene09541:CDS
TGTTACTTGATAACCAATCA+AGG 0.534396 5.2:-8218760 None:intergenic
ATTGGCAAATACAAGTAAAG+TGG 0.538360 5.2:-8218489 None:intergenic
GACTATTTGGAACCTATGGT+TGG 0.543143 5.2:-8217810 None:intergenic
GAAAGAAATGAGGATTCTGA+TGG 0.583316 5.2:+8217716 MS.gene09541:CDS
GAAATGAGGATTCTGATGGT+AGG 0.584694 5.2:+8217720 MS.gene09541:CDS
ACTTGTGCCACGTCTGGACA+AGG 0.593035 5.2:+8218725 MS.gene09541:CDS
TCGTGTAGATTCGACCATTG+TGG 0.593428 5.2:+8218239 MS.gene09541:intron
CAATGCTCAAAGCATTTGGA+AGG 0.595533 5.2:-8218520 None:intergenic
TCTGGACAAGGACTCTATGA+AGG 0.595755 5.2:+8218737 MS.gene09541:CDS
ACGTGACTTACCAACGACGC+TGG 0.609941 5.2:-8218580 None:intergenic
TGAAAATAGTGTCTCCACAA+TGG 0.619967 5.2:-8218253 None:intergenic
ATAGAGTCCTTGTCCAGACG+TGG 0.637305 5.2:-8218732 None:intergenic
GGATGTTGGAGGACAAGACA+AGG 0.648549 5.2:+8217983 MS.gene09541:CDS
AAATGGGGTTGACAGTGTCA+CGG 0.652051 5.2:+8217670 None:intergenic
GTCACTACCATACCAACCAT+AGG 0.654409 5.2:+8217798 MS.gene09541:CDS
ACTTCATAGAATGCTTAGTG+AGG 0.676324 5.2:+8218345 MS.gene09541:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATAGATATTATTTTTCAA+TGG - chr5.2:8217835-8217854 None:intergenic 10.0%
!! TTAATAAGCATTTACATTGT+AGG + chr5.2:8218451-8218470 MS.gene09541:intron 20.0%
!!! TATTTCACTTTTATGTTGAA+AGG - chr5.2:8218417-8218436 None:intergenic 20.0%
!!! TTATTTTTCAATGGACTATT+TGG - chr5.2:8217826-8217845 None:intergenic 20.0%
! TTAAAATGATCATAGGGAAT+AGG - chr5.2:8217865-8217884 None:intergenic 25.0%
! TTTAATGTCTCAAATGAATC+AGG + chr5.2:8217912-8217931 MS.gene09541:intron 25.0%
! TTTATGCAAAGAAAGAAATG+AGG + chr5.2:8217706-8217725 MS.gene09541:CDS 25.0%
! TTTCGCTTAAAATGATCATA+GGG - chr5.2:8217871-8217890 None:intergenic 25.0%
!! ACTTGTTAGAACATATAAGA+AGG - chr5.2:8218167-8218186 None:intergenic 25.0%
!! TTTTCGCTTAAAATGATCAT+AGG - chr5.2:8217872-8217891 None:intergenic 25.0%
AAGCTTCAGATAATGATTCA+TGG - chr5.2:8218614-8218633 None:intergenic 30.0%
ATAATCCAAAGTTGAGTTCA+GGG + chr5.2:8218671-8218690 MS.gene09541:intron 30.0%
ATTGGCAAATACAAGTAAAG+TGG - chr5.2:8218492-8218511 None:intergenic 30.0%
CATAGTTCAATCAAGTGAAA+GGG - chr5.2:8218128-8218147 None:intergenic 30.0%
GCAGAAATTACTGATAAACT+TGG + chr5.2:8218542-8218561 MS.gene09541:CDS 30.0%
GTTAGAACATATAAGAAGGT+TGG - chr5.2:8218163-8218182 None:intergenic 30.0%
TCATAGTTCAATCAAGTGAA+AGG - chr5.2:8218129-8218148 None:intergenic 30.0%
! CACTATTTTCAGAATACACA+AGG + chr5.2:8218265-8218284 MS.gene09541:CDS 30.0%
! TCTAGTTTGCAATATGAACA+TGG - chr5.2:8218041-8218060 None:intergenic 30.0%
! TGTTACTTGATAACCAATCA+AGG - chr5.2:8218763-8218782 None:intergenic 30.0%
ACTTCATAGAATGCTTAGTG+AGG + chr5.2:8218345-8218364 MS.gene09541:CDS 35.0%
AGGTAATTCAGTTTGCACAT+TGG + chr5.2:8218095-8218114 MS.gene09541:intron 35.0%
ATACTCTACAAACTGAAGCT+TGG + chr5.2:8217768-8217787 MS.gene09541:CDS 35.0%
ATTTGGAAGGTCTTGCTTAT+TGG - chr5.2:8218510-8218529 None:intergenic 35.0%
CATAATCCAAAGTTGAGTTC+AGG + chr5.2:8218670-8218689 MS.gene09541:intron 35.0%
CATTCTATGAAGTTCATCTC+TGG - chr5.2:8218338-8218357 None:intergenic 35.0%
GAAAGAAATGAGGATTCTGA+TGG + chr5.2:8217716-8217735 MS.gene09541:CDS 35.0%
TGAAAATAGTGTCTCCACAA+TGG - chr5.2:8218256-8218275 None:intergenic 35.0%
! TACACAAGGTCTTATCTTTG+TGG + chr5.2:8218279-8218298 MS.gene09541:CDS 35.0%
!! AATGGACTATTTGGAACCTA+TGG - chr5.2:8217817-8217836 None:intergenic 35.0%
AAAATGTCAGCTTCACTGTC+TGG + chr5.2:8217961-8217980 MS.gene09541:CDS 40.0%
AAACACCCTGAACTCAACTT+TGG - chr5.2:8218679-8218698 None:intergenic 40.0%
AAATGTCAGCTTCACTGTCT+GGG + chr5.2:8217962-8217981 MS.gene09541:CDS 40.0%
GAAATGAGGATTCTGATGGT+AGG + chr5.2:8217720-8217739 MS.gene09541:CDS 40.0%
! CAATGCTCAAAGCATTTGGA+AGG - chr5.2:8218523-8218542 None:intergenic 40.0%
! GACTATTTGGAACCTATGGT+TGG - chr5.2:8217813-8217832 None:intergenic 40.0%
! TCTGCAATGCTCAAAGCATT+TGG - chr5.2:8218527-8218546 None:intergenic 40.0%
! TTTGGAACCTATGGTTGGTA+TGG - chr5.2:8217808-8217827 None:intergenic 40.0%
GACTCTATGAAGGCCTTGAT+TGG + chr5.2:8218747-8218766 MS.gene09541:CDS 45.0%
GTCACTACCATACCAACCAT+AGG + chr5.2:8217798-8217817 MS.gene09541:CDS 45.0%
GTTGACTGGATGTACCTGAA+CGG - chr5.2:8218707-8218726 None:intergenic 45.0%
TCGTGTAGATTCGACCATTG+TGG + chr5.2:8218239-8218258 MS.gene09541:intron 45.0%
TCTGGACAAGGACTCTATGA+AGG + chr5.2:8218737-8218756 MS.gene09541:CDS 45.0%
!! AGGGTGTTTTTGATCCGTTC+AGG + chr5.2:8218690-8218709 MS.gene09541:intron 45.0%
!! TAATAATAATAATAATAATG+AGG + chr5.2:8218075-8218094 MS.gene09541:intron 5.0%
ATAGAGTCCTTGTCCAGACG+TGG - chr5.2:8218735-8218754 None:intergenic 50.0%
ATGGTAGGTCTTGATGCTGC+TGG + chr5.2:8217735-8217754 MS.gene09541:CDS 50.0%
GGATGTTGGAGGACAAGACA+AGG + chr5.2:8217983-8218002 MS.gene09541:CDS 50.0%
! AGCTTCACTGTCTGGGATGT+TGG + chr5.2:8217969-8217988 MS.gene09541:CDS 50.0%
! TTCACTGTCTGGGATGTTGG+AGG + chr5.2:8217972-8217991 MS.gene09541:CDS 50.0%
ACGTGACTTACCAACGACGC+TGG - chr5.2:8218583-8218602 None:intergenic 55.0%
ACTTGTGCCACGTCTGGACA+AGG + chr5.2:8218725-8218744 MS.gene09541:CDS 55.0%
ATTCACTTCGCCAGCGTCGT+TGG + chr5.2:8218570-8218589 MS.gene09541:CDS 55.0%
CAGTCAACTTGTGCCACGTC+TGG + chr5.2:8218719-8218738 MS.gene09541:CDS 55.0%
! CAGACGTGGCACAAGTTGAC+TGG - chr5.2:8218721-8218740 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 8217672 8218802 8217672 ID=MS.gene09541
chr5.2 mRNA 8217672 8218802 8217672 ID=MS.gene09541.t1;Parent=MS.gene09541
chr5.2 exon 8217672 8217819 8217672 ID=MS.gene09541.t1.exon1;Parent=MS.gene09541.t1
chr5.2 CDS 8217672 8217819 8217672 ID=cds.MS.gene09541.t1;Parent=MS.gene09541.t1
chr5.2 exon 8217934 8218004 8217934 ID=MS.gene09541.t1.exon2;Parent=MS.gene09541.t1
chr5.2 CDS 8217934 8218004 8217934 ID=cds.MS.gene09541.t1;Parent=MS.gene09541.t1
chr5.2 exon 8218247 8218366 8218247 ID=MS.gene09541.t1.exon3;Parent=MS.gene09541.t1
chr5.2 CDS 8218247 8218366 8218247 ID=cds.MS.gene09541.t1;Parent=MS.gene09541.t1
chr5.2 exon 8218473 8218591 8218473 ID=MS.gene09541.t1.exon4;Parent=MS.gene09541.t1
chr5.2 CDS 8218473 8218591 8218473 ID=cds.MS.gene09541.t1;Parent=MS.gene09541.t1
chr5.2 exon 8218712 8218802 8218712 ID=MS.gene09541.t1.exon5;Parent=MS.gene09541.t1
chr5.2 CDS 8218712 8218802 8218712 ID=cds.MS.gene09541.t1;Parent=MS.gene09541.t1
Gene Sequence

>MS.gene09541

ATGGGGTTGACAGTGTCACGGATAATGAGATTATTTTATGCAAAGAAAGAAATGAGGATTCTGATGGTAGGTCTTGATGCTGCTGGAAAAACAACAATACTCTACAAACTGAAGCTTGGAGAAATTGTCACTACCATACCAACCATAGGTTCCAAATAGTCCATTGAAAAATAATATCTATTTATTTTTTCCTATTCCCTATGATCATTTTAAGCGAAAAATGATTGAAAATTGACATAATTTAATGTCTCAAATGAATCAGGCTTCAATGTAGAGACTGTTGAGTACAAAAATGTCAGCTTCACTGTCTGGGATGTTGGAGGACAAGACAAGGTATCATTCTTGAAATGCATTCTTTTCTAGCTTCCATGTTCATATTGCAAACTAGAAATGCATGCTTGTTTAATAATAATAATAATAATGAGGTAATTCAGTTTGCACATTGGTAATACACCCTTTCACTTGATTGAACTATGAACTAATACTAACCAACCTTCTTATATGTTCTAACAAGTATTTCTTGTATGATTCACATGTTACTCTTCTGATATGAGTATATGCTAAATTTCGTGTAGATTCGACCATTGTGGAGACACTATTTTCAGAATACACAAGGTCTTATCTTTGTGGTAGATAGTAATGATAGAGAAAGAATTTTAGAAGCCAGAGATGAACTTCATAGAATGCTTAGTGAGGTTAGTAGCTTTAAAATAGTCTAGTTTTTTAAGTATTACAAAACTTTCCTTTCAACATAAAAGTGAAATATTTTTGCAATCATATTAATAAGCATTTACATTGTAGGATGAACTTCGTGATGCCACTTTACTTGTATTTGCCAATAAGCAAGACCTTCCAAATGCTTTGAGCATTGCAGAAATTACTGATAAACTTGGTTTACATTCACTTCGCCAGCGTCGTTGGTAAGTCACGTTTTACTTACCATGAATCATTATCTGAAGCTTGAAATCATAGATTGAAACTTCAAATGTGAATTACATCATAATCCAAAGTTGAGTTCAGGGTGTTTTTGATCCGTTCAGGTACATCCAGTCAACTTGTGCCACGTCTGGACAAGGACTCTATGAAGGCCTTGATTGGTTATCAAGTAACATATCTAACAAGATAAGATGA

Protein sequence

>MS.gene09541.t1

MGLTVSRIMRLFYAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNVSFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERILEARDELHRMLSEDELRDATLLVFANKQDLPNALSIAEITDKLGLHSLRQRRWYIQSTCATSGQGLYEGLDWLSSNISNKIR