Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09751.t1 | AFK35012.1 | 98.3 | 240 | 4 | 0 | 1 | 240 | 29 | 268 | 1.40E-126 | 462.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09751.t1 | Q05466 | 71.8 | 234 | 53 | 5 | 9 | 240 | 62 | 284 | 3.3e-79 | 296.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09751.t1 | B7FJ62 | 98.3 | 240 | 4 | 0 | 1 | 240 | 57 | 296 | 1.0e-126 | 462.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050146 | MS.gene09751 | 0.974522 | 2.64E-138 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 56 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGAATAATTGATTGGTCTT+TGG | 0.254387 | 5.4:+6665020 | None:intergenic |
| GGAAATGGACGTGGGTGATT+TGG | 0.317565 | 5.4:+6664247 | None:intergenic |
| ATTGGCACTTGCAAAACAAT+TGG | 0.337338 | 5.4:-6664866 | MS.gene09751:CDS |
| GCCCATGGTCCCACAGGAAA+TGG | 0.346020 | 5.4:+6664232 | None:intergenic |
| GTGCTAGACAAGTTGAAGTT+TGG | 0.390504 | 5.4:-6664837 | MS.gene09751:CDS |
| AAGAAGACGCTGAAACTTCA+AGG | 0.402562 | 5.4:-6665059 | MS.gene09751:CDS |
| TCTCTTCTAGAATAATTGAT+TGG | 0.421188 | 5.4:+6665013 | None:intergenic |
| GATGTGAATCGGTTACCTTC+TGG | 0.421492 | 5.4:-6665219 | MS.gene09751:CDS |
| TTTACATGCAGATCGGGATT+CGG | 0.433835 | 5.4:-6665286 | None:intergenic |
| ACACTTGAAACGGTGCTGTT+TGG | 0.443597 | 5.4:+6665162 | None:intergenic |
| TTGGCACTTGCAAAACAATT+GGG | 0.452760 | 5.4:-6664865 | MS.gene09751:CDS |
| GGGCACATGGTAAGAGTTGT+TGG | 0.457834 | 5.4:+6664337 | None:intergenic |
| GTCCATTTCCTGTGGGACCA+TGG | 0.458770 | 5.4:-6664234 | MS.gene09751:CDS |
| TGAAAATAGACGGTTACAAA+AGG | 0.474850 | 5.4:-6664419 | MS.gene09751:CDS |
| TTTACCACTCACACTTGAAA+CGG | 0.484021 | 5.4:+6665152 | None:intergenic |
| TCCATTTCCTGTGGGACCAT+GGG | 0.490692 | 5.4:-6664233 | MS.gene09751:CDS |
| AGAAGCGAAAGAGAAGTTAC+AGG | 0.494456 | 5.4:-6665129 | MS.gene09751:CDS |
| GCAGCATCAACGGCAGATGA+TGG | 0.502736 | 5.4:+6664292 | None:intergenic |
| ATCCGTACAAGTTAAGATCT+AGG | 0.515404 | 5.4:+6664166 | None:intergenic |
| TCATTTGCATGTAGAATTGT+GGG | 0.520585 | 5.4:+6664365 | None:intergenic |
| GTCATTTGCATGTAGAATTG+TGG | 0.521859 | 5.4:+6664364 | None:intergenic |
| TCGATTCCTCGGAGAAGTAA+AGG | 0.525636 | 5.4:+6665240 | None:intergenic |
| TCCGAGGAATCGATGTGAAT+CGG | 0.527394 | 5.4:-6665230 | MS.gene09751:CDS |
| GCTGTGGCCCATGGTCCCAC+AGG | 0.538822 | 5.4:+6664226 | None:intergenic |
| TCAAAGGTCCTATGTTGGAT+AGG | 0.542840 | 5.4:+6664199 | None:intergenic |
| ACGACGCGTGGCAGCATCAA+CGG | 0.548952 | 5.4:+6664282 | None:intergenic |
| ATGGAAAGAGATTGTTCAAG+AGG | 0.550270 | 5.4:-6665093 | MS.gene09751:CDS |
| TGCCACGCGTCGTCATCCTA+TGG | 0.554799 | 5.4:-6664272 | MS.gene09751:CDS |
| ACCGATTCACATCGATTCCT+CGG | 0.556594 | 5.4:+6665229 | None:intergenic |
| CGTGGGTGATTTGGAGCCAT+AGG | 0.560215 | 5.4:+6664256 | None:intergenic |
| CACATGGTAAGAGTTGTTGG+TGG | 0.564954 | 5.4:+6664340 | None:intergenic |
| ATAGGAGCAGCTGTGGCCCA+TGG | 0.566808 | 5.4:+6664217 | None:intergenic |
| GGTCCCACAGGAAATGGACG+TGG | 0.582860 | 5.4:+6664238 | None:intergenic |
| GGTTTCAAAATCGTAGAGCA+AGG | 0.583437 | 5.4:-6664816 | MS.gene09751:intron |
| ACAGCAACACGCTCACAAGA+GGG | 0.586849 | 5.4:+6664316 | None:intergenic |
| ATGTTGGATAGGAGCAGCTG+TGG | 0.588541 | 5.4:+6664210 | None:intergenic |
| CAGCTGCTCCTATCCAACAT+AGG | 0.588914 | 5.4:-6664207 | MS.gene09751:CDS |
| GTGTATCAAAGGTCCTATGT+TGG | 0.591308 | 5.4:+6664194 | None:intergenic |
| CACCCACGTCCATTTCCTGT+GGG | 0.600865 | 5.4:-6664241 | MS.gene09751:CDS |
| AACAGCAACACGCTCACAAG+AGG | 0.612400 | 5.4:+6664315 | None:intergenic |
| ATCTAACGGATGAAAATAGA+CGG | 0.614300 | 5.4:-6664429 | MS.gene09751:CDS |
| TCACCCACGTCCATTTCCTG+TGG | 0.615645 | 5.4:-6664242 | MS.gene09751:CDS |
| TCTAGGACGGAGTGTATCAA+AGG | 0.615968 | 5.4:+6664183 | None:intergenic |
| TCTTCACAATCAGCACCAGA+AGG | 0.621185 | 5.4:+6665204 | None:intergenic |
| ACGCTCACAAGAGGGGCACA+TGG | 0.624285 | 5.4:+6664324 | None:intergenic |
| GCTGATTGTGAAGAAGAAGC+AGG | 0.645646 | 5.4:-6665195 | MS.gene09751:CDS |
| GTACAAGTTAAGATCTAGGA+CGG | 0.655097 | 5.4:+6664170 | None:intergenic |
| AGCCATAGGATGACGACGCG+TGG | 0.657092 | 5.4:+6664270 | None:intergenic |
| TACAGGTGAAGATCTTGACA+TGG | 0.657130 | 5.4:-6665112 | MS.gene09751:CDS |
| CAGCAACACGCTCACAAGAG+GGG | 0.670133 | 5.4:+6664317 | None:intergenic |
| AGCACCGTTTCAAGTGTGAG+TGG | 0.674395 | 5.4:-6665156 | MS.gene09751:CDS |
| AGACATGCAGAATCGAAGAA+CGG | 0.674956 | 5.4:-6665263 | MS.gene09751:CDS |
| GAACGGCCTTTACTTCTCCG+AGG | 0.691005 | 5.4:-6665246 | MS.gene09751:CDS |
| GTCCCACAGGAAATGGACGT+GGG | 0.703771 | 5.4:+6664239 | None:intergenic |
| CATTTGCATGTAGAATTGTG+GGG | 0.706166 | 5.4:+6664366 | None:intergenic |
| AAGATGCTGTGAGAATCTAA+CGG | 0.718794 | 5.4:-6664443 | MS.gene09751:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTAAATTATATTTTTTTTT+AGG | - | chr5.4:6664521-6664540 | MS.gene09751:intron | 0.0% |
| !! | CAATATTTATTTATTTATGT+TGG | - | chr5.4:6664923-6664942 | MS.gene09751:intron | 10.0% |
| !!! | TTATCAATATTTTTTTTTGA+AGG | - | chr5.4:6664894-6664913 | MS.gene09751:intron | 10.0% |
| !! | TTACACTTAATTAATACTTA+CGG | + | chr5.4:6664492-6664511 | None:intergenic | 15.0% |
| !! | TACACTTAATTAATACTTAC+GGG | + | chr5.4:6664491-6664510 | None:intergenic | 20.0% |
| !! | TTGTTTGTAGAAACAAAAAT+TGG | - | chr5.4:6664568-6664587 | MS.gene09751:intron | 20.0% |
| ! | ACACTTAATTAATACTTACG+GGG | + | chr5.4:6664490-6664509 | None:intergenic | 25.0% |
| ! | TTAAAACTCAAAGAGATATC+CGG | - | chr5.4:6664697-6664716 | MS.gene09751:intron | 25.0% |
| !! | GTTTGTTAAATTGTGATTTC+AGG | - | chr5.4:6664945-6664964 | MS.gene09751:intron | 25.0% |
| !!! | TAGAATAATTGATTGGTCTT+TGG | + | chr5.4:6664435-6664454 | None:intergenic | 25.0% |
| !!! | TCTCTTCTAGAATAATTGAT+TGG | + | chr5.4:6664442-6664461 | None:intergenic | 25.0% |
| !!! | TTGACTAGTCTTTATTTAGT+TGG | - | chr5.4:6664668-6664687 | MS.gene09751:intron | 25.0% |
| TCATTTGCATGTAGAATTGT+GGG | + | chr5.4:6665090-6665109 | None:intergenic | 30.0% | |
| TGAAAATAGACGGTTACAAA+AGG | - | chr5.4:6665033-6665052 | MS.gene09751:CDS | 30.0% | |
| ! | ATCTAACGGATGAAAATAGA+CGG | - | chr5.4:6665023-6665042 | MS.gene09751:CDS | 30.0% |
| AAGATGCTGTGAGAATCTAA+CGG | - | chr5.4:6665009-6665028 | MS.gene09751:CDS | 35.0% | |
| ATGGAAAGAGATTGTTCAAG+AGG | - | chr5.4:6664359-6664378 | MS.gene09751:CDS | 35.0% | |
| ATTGGCACTTGCAAAACAAT+TGG | - | chr5.4:6664586-6664605 | MS.gene09751:intron | 35.0% | |
| CATTTGCATGTAGAATTGTG+GGG | + | chr5.4:6665089-6665108 | None:intergenic | 35.0% | |
| GTCATTTGCATGTAGAATTG+TGG | + | chr5.4:6665091-6665110 | None:intergenic | 35.0% | |
| TTGGCACTTGCAAAACAATT+GGG | - | chr5.4:6664587-6664606 | MS.gene09751:intron | 35.0% | |
| TTTACCACTCACACTTGAAA+CGG | + | chr5.4:6664303-6664322 | None:intergenic | 35.0% | |
| TTTCATGTTTGGTATCACGT+TGG | - | chr5.4:6664819-6664838 | MS.gene09751:CDS | 35.0% | |
| ! | GAGATGCATGTTTTCATGTT+TGG | - | chr5.4:6664808-6664827 | MS.gene09751:intron | 35.0% |
| AAGAAGACGCTGAAACTTCA+AGG | - | chr5.4:6664393-6664412 | MS.gene09751:CDS | 40.0% | |
| AGAAGCGAAAGAGAAGTTAC+AGG | - | chr5.4:6664323-6664342 | MS.gene09751:CDS | 40.0% | |
| AGACATGCAGAATCGAAGAA+CGG | - | chr5.4:6664189-6664208 | MS.gene09751:CDS | 40.0% | |
| GGTTTCAAAATCGTAGAGCA+AGG | - | chr5.4:6664636-6664655 | MS.gene09751:intron | 40.0% | |
| GTGTATCAAAGGTCCTATGT+TGG | + | chr5.4:6665261-6665280 | None:intergenic | 40.0% | |
| TACAGGTGAAGATCTTGACA+TGG | - | chr5.4:6664340-6664359 | MS.gene09751:CDS | 40.0% | |
| TCAAAGGTCCTATGTTGGAT+AGG | + | chr5.4:6665256-6665275 | None:intergenic | 40.0% | |
| ! | GTGCTAGACAAGTTGAAGTT+TGG | - | chr5.4:6664615-6664634 | MS.gene09751:intron | 40.0% |
| CACATGGTAAGAGTTGTTGG+TGG | + | chr5.4:6665115-6665134 | None:intergenic | 45.0% | |
| GATGTGAATCGGTTACCTTC+TGG | - | chr5.4:6664233-6664252 | MS.gene09751:CDS | 45.0% | |
| GCTGATTGTGAAGAAGAAGC+AGG | - | chr5.4:6664257-6664276 | MS.gene09751:CDS | 45.0% | |
| TCCGAGGAATCGATGTGAAT+CGG | - | chr5.4:6664222-6664241 | MS.gene09751:CDS | 45.0% | |
| TCGATTCCTCGGAGAAGTAA+AGG | + | chr5.4:6664215-6664234 | None:intergenic | 45.0% | |
| TCTTCACAATCAGCACCAGA+AGG | + | chr5.4:6664251-6664270 | None:intergenic | 45.0% | |
| ! | TCTAGGACGGAGTGTATCAA+AGG | + | chr5.4:6665272-6665291 | None:intergenic | 45.0% |
| !! | ACACTTGAAACGGTGCTGTT+TGG | + | chr5.4:6664293-6664312 | None:intergenic | 45.0% |
| !! | ACCGATTCACATCGATTCCT+CGG | + | chr5.4:6664226-6664245 | None:intergenic | 45.0% |
| AACAGCAACACGCTCACAAG+AGG | + | chr5.4:6665140-6665159 | None:intergenic | 50.0% | |
| ACAGCAACACGCTCACAAGA+GGG | + | chr5.4:6665139-6665158 | None:intergenic | 50.0% | |
| AGAGATATCCGGTGCATGTC+TGG | - | chr5.4:6664708-6664727 | MS.gene09751:intron | 50.0% | |
| ATGTTGGATAGGAGCAGCTG+TGG | + | chr5.4:6665245-6665264 | None:intergenic | 50.0% | |
| CAGCTGCTCCTATCCAACAT+AGG | - | chr5.4:6665245-6665264 | MS.gene09751:CDS | 50.0% | |
| CTGTGATACCAGACATGCAC+CGG | + | chr5.4:6664719-6664738 | None:intergenic | 50.0% | |
| GGAAATGGACGTGGGTGATT+TGG | + | chr5.4:6665208-6665227 | None:intergenic | 50.0% | |
| GGGCACATGGTAAGAGTTGT+TGG | + | chr5.4:6665118-6665137 | None:intergenic | 50.0% | |
| TCCATTTCCTGTGGGACCAT+GGG | - | chr5.4:6665219-6665238 | MS.gene09751:CDS | 50.0% | |
| ! | AGCACCGTTTCAAGTGTGAG+TGG | - | chr5.4:6664296-6664315 | MS.gene09751:CDS | 50.0% |
| CACCCACGTCCATTTCCTGT+GGG | - | chr5.4:6665211-6665230 | MS.gene09751:CDS | 55.0% | |
| CAGCAACACGCTCACAAGAG+GGG | + | chr5.4:6665138-6665157 | None:intergenic | 55.0% | |
| GAACGGCCTTTACTTCTCCG+AGG | - | chr5.4:6664206-6664225 | MS.gene09751:CDS | 55.0% | |
| GCAGCATCAACGGCAGATGA+TGG | + | chr5.4:6665163-6665182 | None:intergenic | 55.0% | |
| GTCCATTTCCTGTGGGACCA+TGG | - | chr5.4:6665218-6665237 | MS.gene09751:CDS | 55.0% | |
| GTCCCACAGGAAATGGACGT+GGG | + | chr5.4:6665216-6665235 | None:intergenic | 55.0% | |
| TCACCCACGTCCATTTCCTG+TGG | - | chr5.4:6665210-6665229 | MS.gene09751:CDS | 55.0% | |
| ! | CGTGGGTGATTTGGAGCCAT+AGG | + | chr5.4:6665199-6665218 | None:intergenic | 55.0% |
| ACGACGCGTGGCAGCATCAA+CGG | + | chr5.4:6665173-6665192 | None:intergenic | 60.0% | |
| AGCCATAGGATGACGACGCG+TGG | + | chr5.4:6665185-6665204 | None:intergenic | 60.0% | |
| ATAGGAGCAGCTGTGGCCCA+TGG | + | chr5.4:6665238-6665257 | None:intergenic | 60.0% | |
| GCCCATGGTCCCACAGGAAA+TGG | + | chr5.4:6665223-6665242 | None:intergenic | 60.0% | |
| GGTCCCACAGGAAATGGACG+TGG | + | chr5.4:6665217-6665236 | None:intergenic | 60.0% | |
| TGCCACGCGTCGTCATCCTA+TGG | - | chr5.4:6665180-6665199 | MS.gene09751:CDS | 60.0% | |
| ! | ACGCTCACAAGAGGGGCACA+TGG | + | chr5.4:6665131-6665150 | None:intergenic | 60.0% |
| GCTGTGGCCCATGGTCCCAC+AGG | + | chr5.4:6665229-6665248 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 6664177 | 6665297 | 6664177 | ID=MS.gene09751 |
| chr5.4 | mRNA | 6664177 | 6665297 | 6664177 | ID=MS.gene09751.t1;Parent=MS.gene09751 |
| chr5.4 | exon | 6664984 | 6665297 | 6664984 | ID=MS.gene09751.t1.exon1;Parent=MS.gene09751.t1 |
| chr5.4 | CDS | 6664984 | 6665297 | 6664984 | ID=cds.MS.gene09751.t1;Parent=MS.gene09751.t1 |
| chr5.4 | exon | 6664817 | 6664896 | 6664817 | ID=MS.gene09751.t1.exon2;Parent=MS.gene09751.t1 |
| chr5.4 | CDS | 6664817 | 6664896 | 6664817 | ID=cds.MS.gene09751.t1;Parent=MS.gene09751.t1 |
| chr5.4 | exon | 6664177 | 6664507 | 6664177 | ID=MS.gene09751.t1.exon3;Parent=MS.gene09751.t1 |
| chr5.4 | CDS | 6664177 | 6664507 | 6664177 | ID=cds.MS.gene09751.t1;Parent=MS.gene09751.t1 |
| Gene Sequence |
| Protein sequence |
