Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050146.t1 | AFK35012.1 | 98.3 | 240 | 4 | 0 | 1 | 240 | 29 | 268 | 1.40E-126 | 462.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050146.t1 | Q05466 | 71.8 | 234 | 53 | 5 | 9 | 240 | 62 | 284 | 3.3e-79 | 296.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050146.t1 | B7FJ62 | 98.3 | 240 | 4 | 0 | 1 | 240 | 57 | 296 | 1.0e-126 | 462.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050146 | MS.gene09749 | 0.973181 | 5.38E-136 | -1.69E-46 |
| MS.gene050146 | MS.gene09751 | 0.974522 | 2.64E-138 | -1.69E-46 |
| MS.gene050146 | MS.gene09924 | 0.975865 | 9.62E-141 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 56 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGAATAATTGATTGGTCTT+TGG | 0.254387 | 5.4:-6593885 | None:intergenic |
| GGAAATGGACGTGGGTGATT+TGG | 0.317565 | 5.4:-6594658 | None:intergenic |
| ATTGGCACTTGCAAAACAAT+TGG | 0.337338 | 5.4:+6594039 | MS.gene050146:CDS |
| GCCCATGGTCCCACAGGAAA+TGG | 0.346020 | 5.4:-6594673 | None:intergenic |
| GTGCTAGACAAGTTGAAGTT+TGG | 0.390504 | 5.4:+6594068 | MS.gene050146:CDS |
| AAGAAGACGCTGAAACTTCA+AGG | 0.402562 | 5.4:+6593846 | MS.gene050146:CDS |
| TCTCTTCTAGAATAATTGAT+TGG | 0.421188 | 5.4:-6593892 | None:intergenic |
| GATGTGAATCGGTTACCTTC+TGG | 0.421492 | 5.4:+6593686 | MS.gene050146:CDS |
| TTTACATGCAGATCGGGATT+CGG | 0.433835 | 5.4:+6593619 | None:intergenic |
| ACACTTGAAACGGTGCTGTT+TGG | 0.443597 | 5.4:-6593743 | None:intergenic |
| TTGGCACTTGCAAAACAATT+GGG | 0.452760 | 5.4:+6594040 | MS.gene050146:CDS |
| GGGCACATGGTAAGAGTTGT+TGG | 0.457834 | 5.4:-6594568 | None:intergenic |
| GTCCATTTCCTGTGGGACCA+TGG | 0.458770 | 5.4:+6594671 | MS.gene050146:CDS |
| TGAAAATAGACGGTTACAAA+AGG | 0.474850 | 5.4:+6594486 | MS.gene050146:CDS |
| TTTACCACTCACACTTGAAA+CGG | 0.484021 | 5.4:-6593753 | None:intergenic |
| TCCATTTCCTGTGGGACCAT+GGG | 0.490692 | 5.4:+6594672 | MS.gene050146:CDS |
| AGAAGCGAAAGAGAAGTTAC+AGG | 0.494456 | 5.4:+6593776 | MS.gene050146:CDS |
| GCAGCATCAACGGCAGATGA+TGG | 0.502736 | 5.4:-6594613 | None:intergenic |
| ATCCGTACAAGTTAAGATCT+AGG | 0.515404 | 5.4:-6594739 | None:intergenic |
| TCATTTGCATGTAGAATTGT+GGG | 0.520585 | 5.4:-6594540 | None:intergenic |
| GTCATTTGCATGTAGAATTG+TGG | 0.521859 | 5.4:-6594541 | None:intergenic |
| TCGATTCCTCGGAGAAGTAA+AGG | 0.525636 | 5.4:-6593665 | None:intergenic |
| TCCGAGGAATCGATGTGAAT+CGG | 0.527394 | 5.4:+6593675 | MS.gene050146:CDS |
| GCTGTGGCCCATGGTCCCAC+AGG | 0.538822 | 5.4:-6594679 | None:intergenic |
| TCAAAGGTCCTATGTTGGAT+AGG | 0.542840 | 5.4:-6594706 | None:intergenic |
| ACGACGCGTGGCAGCATCAA+CGG | 0.548952 | 5.4:-6594623 | None:intergenic |
| ATGGAAAGAGATTGTTCAAG+AGG | 0.550270 | 5.4:+6593812 | MS.gene050146:CDS |
| TGCCACGCGTCGTCATCCTA+TGG | 0.554799 | 5.4:+6594633 | MS.gene050146:CDS |
| ACCGATTCACATCGATTCCT+CGG | 0.556594 | 5.4:-6593676 | None:intergenic |
| CGTGGGTGATTTGGAGCCAT+AGG | 0.560215 | 5.4:-6594649 | None:intergenic |
| CACATGGTAAGAGTTGTTGG+TGG | 0.564954 | 5.4:-6594565 | None:intergenic |
| ATAGGAGCAGCTGTGGCCCA+TGG | 0.566808 | 5.4:-6594688 | None:intergenic |
| GGTCCCACAGGAAATGGACG+TGG | 0.582860 | 5.4:-6594667 | None:intergenic |
| GGTTTCAAAATCGTAGAGCA+AGG | 0.583437 | 5.4:+6594089 | MS.gene050146:CDS |
| ACAGCAACACGCTCACAAGA+GGG | 0.586849 | 5.4:-6594589 | None:intergenic |
| ATGTTGGATAGGAGCAGCTG+TGG | 0.588541 | 5.4:-6594695 | None:intergenic |
| CAGCTGCTCCTATCCAACAT+AGG | 0.588914 | 5.4:+6594698 | MS.gene050146:CDS |
| GTGTATCAAAGGTCCTATGT+TGG | 0.591308 | 5.4:-6594711 | None:intergenic |
| CACCCACGTCCATTTCCTGT+GGG | 0.600865 | 5.4:+6594664 | MS.gene050146:CDS |
| AACAGCAACACGCTCACAAG+AGG | 0.612400 | 5.4:-6594590 | None:intergenic |
| ATCTAACGGATGAAAATAGA+CGG | 0.614300 | 5.4:+6594476 | MS.gene050146:CDS |
| TCACCCACGTCCATTTCCTG+TGG | 0.615645 | 5.4:+6594663 | MS.gene050146:CDS |
| TCTAGGACGGAGTGTATCAA+AGG | 0.615968 | 5.4:-6594722 | None:intergenic |
| TCTTCACAATCAGCACCAGA+AGG | 0.621185 | 5.4:-6593701 | None:intergenic |
| ACGCTCACAAGAGGGGCACA+TGG | 0.624285 | 5.4:-6594581 | None:intergenic |
| GCTGATTGTGAAGAAGAAGC+AGG | 0.645646 | 5.4:+6593710 | MS.gene050146:CDS |
| GTACAAGTTAAGATCTAGGA+CGG | 0.655097 | 5.4:-6594735 | None:intergenic |
| AGCCATAGGATGACGACGCG+TGG | 0.657092 | 5.4:-6594635 | None:intergenic |
| TACAGGTGAAGATCTTGACA+TGG | 0.657130 | 5.4:+6593793 | MS.gene050146:CDS |
| CAGCAACACGCTCACAAGAG+GGG | 0.670133 | 5.4:-6594588 | None:intergenic |
| AGCACCGTTTCAAGTGTGAG+TGG | 0.674395 | 5.4:+6593749 | MS.gene050146:CDS |
| AGACATGCAGAATCGAAGAA+CGG | 0.674956 | 5.4:+6593642 | MS.gene050146:CDS |
| GAACGGCCTTTACTTCTCCG+AGG | 0.691005 | 5.4:+6593659 | MS.gene050146:CDS |
| GTCCCACAGGAAATGGACGT+GGG | 0.703771 | 5.4:-6594666 | None:intergenic |
| CATTTGCATGTAGAATTGTG+GGG | 0.706166 | 5.4:-6594539 | None:intergenic |
| AAGATGCTGTGAGAATCTAA+CGG | 0.718794 | 5.4:+6594462 | MS.gene050146:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTAAATTATATTTTTTTTT+AGG | + | chr5.4:6593974-6593993 | MS.gene050146:intron | 0.0% |
| !! | CAATATTTATTTATTTATGT+TGG | + | chr5.4:6594376-6594395 | MS.gene050146:intron | 10.0% |
| !!! | TTATCAATATTTTTTTTTGA+AGG | + | chr5.4:6594347-6594366 | MS.gene050146:intron | 10.0% |
| !! | TTACACTTAATTAATACTTA+CGG | - | chr5.4:6593945-6593964 | None:intergenic | 15.0% |
| !! | TACACTTAATTAATACTTAC+GGG | - | chr5.4:6593944-6593963 | None:intergenic | 20.0% |
| !! | TTGTTTGTAGAAACAAAAAT+TGG | + | chr5.4:6594021-6594040 | MS.gene050146:intron | 20.0% |
| ! | ACACTTAATTAATACTTACG+GGG | - | chr5.4:6593943-6593962 | None:intergenic | 25.0% |
| ! | TTAAAACTCAAAGAGATATC+CGG | + | chr5.4:6594150-6594169 | MS.gene050146:intron | 25.0% |
| !! | GTTTGTTAAATTGTGATTTC+AGG | + | chr5.4:6594398-6594417 | MS.gene050146:intron | 25.0% |
| !!! | TAGAATAATTGATTGGTCTT+TGG | - | chr5.4:6593888-6593907 | None:intergenic | 25.0% |
| !!! | TCTCTTCTAGAATAATTGAT+TGG | - | chr5.4:6593895-6593914 | None:intergenic | 25.0% |
| !!! | TTGACTAGTCTTTATTTAGT+TGG | + | chr5.4:6594121-6594140 | MS.gene050146:intron | 25.0% |
| TCATTTGCATGTAGAATTGT+GGG | - | chr5.4:6594543-6594562 | None:intergenic | 30.0% | |
| TGAAAATAGACGGTTACAAA+AGG | + | chr5.4:6594486-6594505 | MS.gene050146:CDS | 30.0% | |
| ! | ATCTAACGGATGAAAATAGA+CGG | + | chr5.4:6594476-6594495 | MS.gene050146:CDS | 30.0% |
| AAGATGCTGTGAGAATCTAA+CGG | + | chr5.4:6594462-6594481 | MS.gene050146:CDS | 35.0% | |
| ATGGAAAGAGATTGTTCAAG+AGG | + | chr5.4:6593812-6593831 | MS.gene050146:CDS | 35.0% | |
| ATTGGCACTTGCAAAACAAT+TGG | + | chr5.4:6594039-6594058 | MS.gene050146:CDS | 35.0% | |
| CATTTGCATGTAGAATTGTG+GGG | - | chr5.4:6594542-6594561 | None:intergenic | 35.0% | |
| GTCATTTGCATGTAGAATTG+TGG | - | chr5.4:6594544-6594563 | None:intergenic | 35.0% | |
| TTGGCACTTGCAAAACAATT+GGG | + | chr5.4:6594040-6594059 | MS.gene050146:CDS | 35.0% | |
| TTTACCACTCACACTTGAAA+CGG | - | chr5.4:6593756-6593775 | None:intergenic | 35.0% | |
| TTTCATGTTTGGTATCACGT+TGG | + | chr5.4:6594272-6594291 | MS.gene050146:intron | 35.0% | |
| ! | GAGATGCATGTTTTCATGTT+TGG | + | chr5.4:6594261-6594280 | MS.gene050146:intron | 35.0% |
| AAGAAGACGCTGAAACTTCA+AGG | + | chr5.4:6593846-6593865 | MS.gene050146:CDS | 40.0% | |
| AGAAGCGAAAGAGAAGTTAC+AGG | + | chr5.4:6593776-6593795 | MS.gene050146:CDS | 40.0% | |
| AGACATGCAGAATCGAAGAA+CGG | + | chr5.4:6593642-6593661 | MS.gene050146:CDS | 40.0% | |
| GGTTTCAAAATCGTAGAGCA+AGG | + | chr5.4:6594089-6594108 | MS.gene050146:CDS | 40.0% | |
| GTGTATCAAAGGTCCTATGT+TGG | - | chr5.4:6594714-6594733 | None:intergenic | 40.0% | |
| TACAGGTGAAGATCTTGACA+TGG | + | chr5.4:6593793-6593812 | MS.gene050146:CDS | 40.0% | |
| TCAAAGGTCCTATGTTGGAT+AGG | - | chr5.4:6594709-6594728 | None:intergenic | 40.0% | |
| ! | GTGCTAGACAAGTTGAAGTT+TGG | + | chr5.4:6594068-6594087 | MS.gene050146:CDS | 40.0% |
| CACATGGTAAGAGTTGTTGG+TGG | - | chr5.4:6594568-6594587 | None:intergenic | 45.0% | |
| GATGTGAATCGGTTACCTTC+TGG | + | chr5.4:6593686-6593705 | MS.gene050146:CDS | 45.0% | |
| GCTGATTGTGAAGAAGAAGC+AGG | + | chr5.4:6593710-6593729 | MS.gene050146:CDS | 45.0% | |
| TCCGAGGAATCGATGTGAAT+CGG | + | chr5.4:6593675-6593694 | MS.gene050146:CDS | 45.0% | |
| TCGATTCCTCGGAGAAGTAA+AGG | - | chr5.4:6593668-6593687 | None:intergenic | 45.0% | |
| TCTTCACAATCAGCACCAGA+AGG | - | chr5.4:6593704-6593723 | None:intergenic | 45.0% | |
| ! | TCTAGGACGGAGTGTATCAA+AGG | - | chr5.4:6594725-6594744 | None:intergenic | 45.0% |
| !! | ACACTTGAAACGGTGCTGTT+TGG | - | chr5.4:6593746-6593765 | None:intergenic | 45.0% |
| !! | ACCGATTCACATCGATTCCT+CGG | - | chr5.4:6593679-6593698 | None:intergenic | 45.0% |
| AACAGCAACACGCTCACAAG+AGG | - | chr5.4:6594593-6594612 | None:intergenic | 50.0% | |
| ACAGCAACACGCTCACAAGA+GGG | - | chr5.4:6594592-6594611 | None:intergenic | 50.0% | |
| AGAGATATCCGGTGCATGTC+TGG | + | chr5.4:6594161-6594180 | MS.gene050146:intron | 50.0% | |
| ATGTTGGATAGGAGCAGCTG+TGG | - | chr5.4:6594698-6594717 | None:intergenic | 50.0% | |
| CAGCTGCTCCTATCCAACAT+AGG | + | chr5.4:6594698-6594717 | MS.gene050146:CDS | 50.0% | |
| CTGTGATACCAGACATGCAC+CGG | - | chr5.4:6594172-6594191 | None:intergenic | 50.0% | |
| GGAAATGGACGTGGGTGATT+TGG | - | chr5.4:6594661-6594680 | None:intergenic | 50.0% | |
| GGGCACATGGTAAGAGTTGT+TGG | - | chr5.4:6594571-6594590 | None:intergenic | 50.0% | |
| TCCATTTCCTGTGGGACCAT+GGG | + | chr5.4:6594672-6594691 | MS.gene050146:CDS | 50.0% | |
| ! | AGCACCGTTTCAAGTGTGAG+TGG | + | chr5.4:6593749-6593768 | MS.gene050146:CDS | 50.0% |
| CACCCACGTCCATTTCCTGT+GGG | + | chr5.4:6594664-6594683 | MS.gene050146:CDS | 55.0% | |
| CAGCAACACGCTCACAAGAG+GGG | - | chr5.4:6594591-6594610 | None:intergenic | 55.0% | |
| GAACGGCCTTTACTTCTCCG+AGG | + | chr5.4:6593659-6593678 | MS.gene050146:CDS | 55.0% | |
| GCAGCATCAACGGCAGATGA+TGG | - | chr5.4:6594616-6594635 | None:intergenic | 55.0% | |
| GTCCATTTCCTGTGGGACCA+TGG | + | chr5.4:6594671-6594690 | MS.gene050146:CDS | 55.0% | |
| GTCCCACAGGAAATGGACGT+GGG | - | chr5.4:6594669-6594688 | None:intergenic | 55.0% | |
| TCACCCACGTCCATTTCCTG+TGG | + | chr5.4:6594663-6594682 | MS.gene050146:CDS | 55.0% | |
| ! | CGTGGGTGATTTGGAGCCAT+AGG | - | chr5.4:6594652-6594671 | None:intergenic | 55.0% |
| ACGACGCGTGGCAGCATCAA+CGG | - | chr5.4:6594626-6594645 | None:intergenic | 60.0% | |
| AGCCATAGGATGACGACGCG+TGG | - | chr5.4:6594638-6594657 | None:intergenic | 60.0% | |
| ATAGGAGCAGCTGTGGCCCA+TGG | - | chr5.4:6594691-6594710 | None:intergenic | 60.0% | |
| GCCCATGGTCCCACAGGAAA+TGG | - | chr5.4:6594676-6594695 | None:intergenic | 60.0% | |
| GGTCCCACAGGAAATGGACG+TGG | - | chr5.4:6594670-6594689 | None:intergenic | 60.0% | |
| TGCCACGCGTCGTCATCCTA+TGG | + | chr5.4:6594633-6594652 | MS.gene050146:CDS | 60.0% | |
| ! | ACGCTCACAAGAGGGGCACA+TGG | - | chr5.4:6594584-6594603 | None:intergenic | 60.0% |
| GCTGTGGCCCATGGTCCCAC+AGG | - | chr5.4:6594682-6594701 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 6593630 | 6594750 | 6593630 | ID=MS.gene050146 |
| chr5.4 | mRNA | 6593630 | 6594750 | 6593630 | ID=MS.gene050146.t1;Parent=MS.gene050146 |
| chr5.4 | exon | 6593630 | 6593943 | 6593630 | ID=MS.gene050146.t1.exon1;Parent=MS.gene050146.t1 |
| chr5.4 | CDS | 6593630 | 6593943 | 6593630 | ID=cds.MS.gene050146.t1;Parent=MS.gene050146.t1 |
| chr5.4 | exon | 6594031 | 6594110 | 6594031 | ID=MS.gene050146.t1.exon2;Parent=MS.gene050146.t1 |
| chr5.4 | CDS | 6594031 | 6594110 | 6594031 | ID=cds.MS.gene050146.t1;Parent=MS.gene050146.t1 |
| chr5.4 | exon | 6594420 | 6594750 | 6594420 | ID=MS.gene050146.t1.exon3;Parent=MS.gene050146.t1 |
| chr5.4 | CDS | 6594420 | 6594750 | 6594420 | ID=cds.MS.gene050146.t1;Parent=MS.gene050146.t1 |
| Gene Sequence |
| Protein sequence |
